JPH02119790A - Production of xylobiose - Google Patents
Production of xylobioseInfo
- Publication number
- JPH02119790A JPH02119790A JP63274785A JP27478588A JPH02119790A JP H02119790 A JPH02119790 A JP H02119790A JP 63274785 A JP63274785 A JP 63274785A JP 27478588 A JP27478588 A JP 27478588A JP H02119790 A JPH02119790 A JP H02119790A
- Authority
- JP
- Japan
- Prior art keywords
- xylobiose
- xylan
- xylanase
- enzyme
- chaetomium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- LGQKSQQRKHFMLI-SJYYZXOBSA-N (2s,3r,4s,5r)-2-[(3r,4r,5r,6r)-4,5,6-trihydroxyoxan-3-yl]oxyoxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)OC1 LGQKSQQRKHFMLI-SJYYZXOBSA-N 0.000 title claims abstract description 27
- LGQKSQQRKHFMLI-UHFFFAOYSA-N 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(O)OC1 LGQKSQQRKHFMLI-UHFFFAOYSA-N 0.000 title claims abstract description 27
- SQNRKWHRVIAKLP-UHFFFAOYSA-N D-xylobiose Natural products O=CC(O)C(O)C(CO)OC1OCC(O)C(O)C1O SQNRKWHRVIAKLP-UHFFFAOYSA-N 0.000 title claims abstract description 27
- 238000004519 manufacturing process Methods 0.000 title claims description 14
- 229920001221 xylan Polymers 0.000 claims abstract description 29
- 150000004823 xylans Chemical class 0.000 claims abstract description 27
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims abstract description 24
- 241000221955 Chaetomium Species 0.000 claims abstract description 8
- 244000005700 microbiome Species 0.000 claims abstract description 8
- 108090000790 Enzymes Proteins 0.000 claims description 27
- 102000004190 Enzymes Human genes 0.000 claims description 27
- 238000002360 preparation method Methods 0.000 claims description 6
- 229920001542 oligosaccharide Polymers 0.000 claims description 5
- 238000012258 culturing Methods 0.000 abstract description 4
- 235000013305 food Nutrition 0.000 abstract description 4
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- 230000001629 suppression Effects 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 26
- 239000003795 chemical substances by application Substances 0.000 description 16
- 230000000694 effects Effects 0.000 description 16
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 15
- 235000000346 sugar Nutrition 0.000 description 14
- 238000000354 decomposition reaction Methods 0.000 description 10
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 9
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 9
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 239000000758 substrate Substances 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000002255 enzymatic effect Effects 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 239000002994 raw material Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 5
- 235000018185 Betula X alpestris Nutrition 0.000 description 4
- 235000018212 Betula X uliginosa Nutrition 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- PCLIMKBDDGJMGD-UHFFFAOYSA-N N-bromosuccinimide Chemical compound BrN1C(=O)CCC1=O PCLIMKBDDGJMGD-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
- 235000012054 meals Nutrition 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 229940079919 digestives enzyme preparation Drugs 0.000 description 3
- 238000006911 enzymatic reaction Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 150000008163 sugars Chemical class 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 241000635201 Pumilus Species 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000010903 husk Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- XMKLTEGSALONPH-UHFFFAOYSA-N 1,2,4,5-tetrazinane-3,6-dione Chemical compound O=C1NNC(=O)NN1 XMKLTEGSALONPH-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- JCSJTDYCNQHPRJ-UHFFFAOYSA-N 20-hydroxyecdysone 2,3-acetonide Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(OC2C(C(O)C(O)OC2)O)OC1 JCSJTDYCNQHPRJ-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 241000609240 Ambelania acida Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 101710132690 Endo-1,4-beta-xylanase A Proteins 0.000 description 1
- 240000008620 Fagopyrum esculentum Species 0.000 description 1
- 235000009419 Fagopyrum esculentum Nutrition 0.000 description 1
- 241000218652 Larix Species 0.000 description 1
- 235000005590 Larix decidua Nutrition 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 244000197975 Solidago virgaurea Species 0.000 description 1
- 235000000914 Solidago virgaurea Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000010905 bagasse Substances 0.000 description 1
- JCSJTDYCNQHPRJ-FDVJSPBESA-N beta-D-Xylp-(1->4)-beta-D-Xylp-(1->4)-D-Xylp Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)C(O)OC2)O)OC1 JCSJTDYCNQHPRJ-FDVJSPBESA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- -1 cottonseed husk Chemical class 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 208000002925 dental caries Diseases 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 150000002009 diols Chemical class 0.000 description 1
- 108010038658 exo-1,4-beta-D-xylosidase Proteins 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000005417 food ingredient Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 229910000358 iron sulfate Inorganic materials 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- YFZOUMNUDGGHIW-UHFFFAOYSA-M p-chloromercuribenzoic acid Chemical compound OC(=O)C1=CC=C([Hg]Cl)C=C1 YFZOUMNUDGGHIW-UHFFFAOYSA-M 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000009495 sugar coating Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 125000000969 xylosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)CO1)* 0.000 description 1
- ABKNGTPZXRUSOI-UHFFFAOYSA-N xylotriose Natural products OCC(OC1OCC(OC2OCC(O)C(O)C2O)C(O)C1O)C(O)C(O)C=O ABKNGTPZXRUSOI-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は酵素分留法１．二よるえシロビオ−人糖衣の製
造法に関する。DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention relates to an enzymatic fractionation method 1. Niyorue Silobio - Concerning the manufacturing method of human sugar coating.
（従来の技術）
食品の柔軟性、嗜好性、抗う触性、および感触その池の
、１＾質を改良するｖＡ″ｉ！Ｉとして、可溶性の糖質
で什味か少ないキシロオリゴ糖がフル１オリコ糖と同様
にＪυｊ持されており、特に食品のカロリー抑制、水分
活性調整という点ではキシロビオースかすぐれている。(Prior art) Xylo-oligosaccharides, which are soluble carbohydrates and have little flavor, are used to improve the softness, palatability, caries resistance, and texture of foods. Like olicosaccharide, it has a high Jυj content, and is superior to xylobiose in terms of reducing calories in foods and regulating water activity.
しかし、キシロビオ−・スについては原料キシランの酸
分解に上る方法は勿論、酵素による分解法であってもこ
れを効率よく工業的に分取することは従米極め′ζ困難
であった。従って原料のキシランが自然界に多量に存在
している、こ拘わらずキシロビオースの製造フス）か著
しく高く、食品素イイとして期待される諸効果を検詞す
るための試験材料さえも充分に使用出来ないのか現状で
ある。However, it has been extremely difficult to efficiently extract xylobiobioses industrially, not only by methods that involve acid decomposition of raw material xylan, but also by enzymatic decomposition methods. Therefore, although the raw material xylan exists in large quantities in nature, the production rate of xylobiose is extremely high, making it impossible to use enough test materials to determine the various effects expected as a good food ingredient. This is the current situation.
（発明が解決しようとする問題点・本発明の目的）本発
明の特定発明１土ケｌミウム属に属し、キシラナーゼ生
産能を有する微生物の酵素を各種のキシランに作用させ
ることにより、キシロビオースな・主体とするキシロオ
リゴ糖を生成させ、その生成物からキシロビオースを採
取することを目的とするキシロビオースの製造法に係る
ものである。(Problems to be Solved by the Invention/Objects of the Invention) Specific Inventions of the Invention 1. Xylobiose and The present invention relates to a method for producing xylobiose, which aims to produce xylo-oligosaccharide as a main ingredient and collect xylobiose from the product.
また本発明はケ１ミウム・グラシルＩＦ０６５６８又は
ぞの変異菌株の産生するキシラナーゼを採取し、これを
キシラン原ＩＩに作用させることによりキシロースやグ
ルコースなどの単Ｍ類の生成を極力抑え、キシロビオー
スを主体とするキシロオリゴ糖を効率よく製造１−るこ
とを目的とするものであって、実質的には前記の特定発
明の実施態様発明を構成するものである。In addition, the present invention collects the xylanase produced by C. gracil IF06568 or its mutant strain and makes it act on xylan origin II to suppress the production of single M species such as xylose and glucose as much as possible, and to produce mainly xylobiose. The object of the present invention is to efficiently produce xylo-oligosaccharides, and substantially constitutes an embodiment of the above-mentioned specific invention.
（問題点を解決するための手段）
本発明は前記した問題点の解決のｌ］的にａ致するもの
で、ゲトミウム（ＣＩ＋ ａ ｅ Ｌ ｏ ＩｆｌＬｌ
ｔ□）属に属し、キシラナーゼ生産能を有する微生物
を培養して酵素剤を調製し、これを各種のキンランに作
用させることによりキシロビオースを主体とするキシロ
オリゴ糖を生成させ、その生成物からキシロビオースを
採取することを特徴とするキシロビオースの製造（こあ
る。また、ケ）ミ・ンム・グラシル ＩＦ０６５６８又
はその変異イネを培養してなる酵素剤を、キシラン含有
植物体より分離抽出したキシランに作用させることによ
り、キシロースの生成を抑えで、高濃度のキシロビオー
スを含有する糖液を製造することにある。(Means for Solving the Problems) The present invention is intended to solve the above-mentioned problems.
A microorganism that belongs to the genus T□) and has the ability to produce xylanase is cultured to prepare an enzyme preparation, and this is applied to various types of goldenrod to produce xylo-oligosaccharides mainly composed of xylobiose. Production of xylobiose, which is characterized in that it is collected. The objective is to suppress the production of xylose and produce a sugar solution containing a high concentration of xylobiose.
本発明者らは、各種キシランを基質として、キシロオリ
ゴ糖を効率よく生成させる酵素剤の検索を行ってぎたと
ころ、ケトミウム・グラシルＩＦ０６５６８ （本菌株
はデキスｌラナーゼ産生菌として工業的に使用されてい
る。又ＡＴＣＣ１６１５３は本菌株と同一菌株である。The present inventors searched for an enzyme agent that efficiently produces xylo-oligosaccharides using various xylans as substrates, and found that Chaetomium glacil IF06568 (this strain is used industrially as a dexllanase-producing bacterium) Also, ATCC16153 is the same strain as this strain.
）が特異的にキシロビオースを多く生成する一力、Ａ−
シロース、グルコース及びキシロトリオースの生成が僅
がであるキシラナーゼを産生することを見い出した。) is the one that specifically produces a large amount of xylobiose, A-
It has been found that the production of sylose, glucose and xylotriose produces only a small amount of xylanase.
本発明に用いる菌株ケｌミウム・グラシルＩＦＯ６５６
８は例えば財団法人醗酵研究所から制限なく入手するこ
とができる８
本発明はこの菌株のみに限らず変異株も含まれる。即ち
本発明者らは、上記のようにケ）ミウム・グラシルＩＦ
０６５６８が特異なキシラナーゼを産生することを見い
出したので、ケ１−ミウム属に属し、キシラナーゼ生産
能を有する微生物を培養した酵素剤を８種のキシランに
作用させることを特定発明にＹると共に、前記の菌株′
ｆｌ ミウム・グラシルを原体として変異処Ｆｌ！を施
し、より高力価の液化型キシラナーゼを産生する人工変
異株の造成も行い、キシロビオース生成能の者しくすぐ
れた異変株、例えば旧０−１６２、Ｒ１５−６２１、Ｒ
１５−１１，６１などを得ることができだ。Bacterial strain Kelmium gracil IFO656 used in the present invention
8 can be obtained without restriction from the Fermentation Research Institute, for example. The present invention is not limited to this strain, but also includes mutant strains. That is, the present inventors, as described above,
06568 was found to produce a specific xylanase, and therefore, we have determined that it is a specific invention to make an enzyme preparation obtained by culturing a microorganism belonging to the genus Chemium and having the ability to produce xylanase to act on eight types of xylan. Said strain'
fl Mutated using Mium Gracil as the original substance Fl! We also created artificial mutant strains that produce higher titers of liquefied xylanase, and created mutant strains with excellent xylobiose production ability, such as old 0-162, R15-621, and R.
You can get 15-11, 61, etc.
ケ）ミ゛ンム・グラシル ＩＦＯ６５６８およびその人
工式変株Ｐ、１５−１１．６１のキシラナーゼについて
Ｐｌ＋依存性、温度依存性、ｐＨ安定性、耐熱性等の測
定を行い第１〜４図の測定値を得た。第１図は、バ−チ
ウ／ドキシラン（ＤＷＸ）を基質にして４０’Ｃ−３０
＋ｆｌｉｎの条件て”酵素反応を行す・、生成した還元
糖をネ／シソン・ソモギ法で測定した門Ｉ依存性のグラ
フ。第２図は前記基質液をＩ’ Ｈ５、Ｏ及び門１５．
５で分解した温度依存性のグラフ。第３図は各Ｐｌ＋の
緩衝液に酵素を溶解し、３０°Ｃ２０１目・処理後、残
存活性を測定したＩ’１１安定性のグラフ。第４図はＭ
、’ ２０酎酸緩衝液（Ｐｔ１５．ｏ）ｔこ前記酵素
を溶解し、３０ ’−” ５０°Ｃ４ｈｒで処理して測
定した耐熱性のグラフである。iii) Pl+ dependence, temperature dependence, pH stability, heat resistance, etc. of the xylanase of Minimum Glycil IFO6568 and its artificial variant P, 15-11.61 were measured, and the measurements shown in Figures 1 to 4 were carried out. Got the value. Figure 1 shows 40'C-30 using birch/doxylan (DWX) as a substrate.
A graph of the gate I dependence of the produced reducing sugars measured by the Ne/Sison-Somogi method when the enzymatic reaction is carried out under the conditions of +flin.
Graph of temperature dependence decomposed in step 5. FIG. 3 is a graph of I'11 stability obtained by dissolving the enzyme in each Pl+ buffer and measuring the residual activity after treatment at 30°C. Figure 4 is M
, 20 is a graph of heat resistance measured by dissolving the enzyme in oxic acid buffer (Pt15.o) and treating it at 50°C for 4 hours.
なお、本発明による菌株のキシラナーゼについては、さ
らに次のようにして ＪＪ、下（■へ■）の諸特性も明
らかにした。ＵＪｌち
■ 分子量 Ｔ Ｓ Ｋ −ｇｅｌ Ｇ ３０００
Ｓ Ｗを用いた分子ふるいクロマトグラフィー
により約８，６００と判定。In addition, regarding the xylanase of the strain according to the present invention, various characteristics of JJ, below (■ to ■) were further clarified as follows. Molecular weight TSK-gel G 3000
Determined to be approximately 8,600 by molecular sieve chromatography using SW.
・■ 等電点 アガロースＩＥＦデルを用り）た等電
点電気泳動法によＱ８．３５と判定。・■ Isoelectric point It was determined to be Q8.35 by isoelectric focusing method (using agarose IEF del).
■ 金属イオ ｌ−１８２＋及びＣｄ’＋により１■害
矛れンの影響 る。■Metal ions l-182+ and Cd'+ cause 1■ harmful effects.
・■ 活性■害 ５ＤＳ（ドデシル硫酸す１リウム）剤
のｉｔｚ’Ｊ ＮＢＳ（Ｎ−ブロモこはく酸イミド）
Ｐ−（ｌｉＢ（Ｐ−クロロマーキュリ−ベンゾイックア
シノド）により活
性が阻害される。・■Activity■Harm 5DS (sodium dodecyl sulfate) agent itz'J NBS (N-bromosuccinimide)
The activity is inhibited by P-(liB (P-chloromercury-benzoic acid).
Ｅ Ｄ Ｔ Ａ （エチレンノアミン四耐酸）は活性を
阻害しない。EDT A (ethylenenoaminetetraacid) does not inhibit activity.
本発明は以上に述べた酵素化学的性質のほか、これらの
菌株について、その菌学的諸性質、培養条件、産生する
キシラナーゼの各種キシランに月する効果的な分解反応
条件などを詳細に研究した結果、完成したものである。In addition to the enzymatic chemical properties described above, the present invention has been carried out by detailed research on the mycological properties, culture conditions, and effective decomposition reaction conditions for the xylanase produced by various types of xylan. As a result, it is completed.
各種微生物が産生するキシラナーゼＩこ関して１よ数多
くの報告があり、既に工業生産され実用（こ供されてい
るものがあるが、ケ）ミウム属のキシラナーゼに関して
は報告も極めて少なし・。即ち福井＄（木、）ノケトミ
ウム・グロホ゛サム（ＣＩ’＋ ａ ｅ ｔ ｏ Ｉｎ
＋ ｕ ＩＩｌン）ｌｏｌ＋ｏｓｕｍ約）はエヘーソ
７ｉ、＋１キシラナーゼを産生してβ−１，３−キシラ
ンを特異的に分解する。又、用南等（＊；：）のケトミ
ウム・１リラテラ−レ（ＣＬａｓしｏ＋ａｉｕｍ Ｌ
ｒｉ １ａＬｃｒａｌｃ ５ｔｒａｉｎ Ｎｏ、２２Ｃ
ｉ４）は、キシロースを主たる最終生成物とするエンド
型キシラナーゼ（本体はβ−キシロシダーゼ）であって
、分子−量は２４０，０００又は１１８，０００ 等
電点け４．８６であり（木、）本発明者らのケ）ミツム
・グラシルによるβ−１，４−キシランを分解してキシ
ロビオースを主たる最終生成物とするエンド型キシラナ
ーゼとは明らかに異なる。There have been many reports on xylanase I produced by various microorganisms, and some have already been industrially produced and put into practical use, but there are very few reports on xylanase from the genus Chemium. Namely, Fukui $ (Thursday) Nochaetomium glophosum (CI'+a et to In
+uIIln)lol+osum) produces eheso7i, +1 xylanase to specifically degrade β-1,3-xylan. In addition, Chaetomium 1 lilatella (CLas + aium L) by Yonan et al. (*;:)
ri 1aLcralc 5train No.22C
i4) is an endo-type xylanase (the main body is β-xylosidase) whose main end product is xylose, has a molecular weight of 240,000 or 118,000, an isoelectric point of 4.86, and has a molecular weight of 240,000 or 118,000. It is clearly different from the endo-type xylanase, which decomposes β-1,4-xylan and produces xylobiose as the main final product, which was developed by the inventors.
４８、１６３ 、１ ６９ （１，９７０）＊ ３
Ｎ、Ｕｚｉｉｅ、 Ｈ，１４ａＬｕｏ、 Ｔ、Ｙ
ａｓｕｉ ；八Ｈｒｉｃ。48, 163, 1 69 (1,970) * 3
N, Uziie, H, 14aLuo, T, Y
asui; eight Hric.
Ｄｉｏｌ １Ｃｌ＋ｃ＋ｎ、 ４ ９ 、 １ １
５ ９ （１９８５）基質とする原料キシランは可
溶性、不溶性どちらでもよく、例えは綿実殻、１−ウモ
ロコシの穂軸、麦芽粕、ソバ穀、バガス、シラカバ、カ
ラマツ等の各種のキシランを原料とし、好適な温度、ｐ
Ｈ域で本酵素剤を作用させることにより、キシロビオス
を特異的に生成することがでとる。Diol 1Cl+c+n, 4 9, 1 1
5 9 (1985) The raw material xylan used as a substrate may be either soluble or insoluble. For example, various types of xylan such as cottonseed husk, corn cob, malt cake, buckwheat grain, bagasse, birch, and larch can be used as raw materials. , suitable temperature, p
By acting this enzyme agent in the H region, xylobios can be specifically produced.
本発明の菌株の培養は液体培地又は固体培地の何れによ
ってもよい。The strain of the present invention may be cultured in either a liquid medium or a solid medium.
液体培地の場合には炭素源としてグルコース、７ラタト
ース、キシロース、すグルコース、マルトース、可溶性
デンプン、糖蜜、マンニトール等の・船釣に使用さｔし
ているものか使用で゛ぎるか、これに限られるものでは
ない。In the case of a liquid medium, carbon sources such as glucose, 7-ratatose, xylose, sugar glucose, maltose, soluble starch, molasses, mannitol, etc., are used in boat fishing, are used too much, or are limited to these. It's not something you can do.
窒素源としではベグ１ン、酵母エキス、麦芽エキス、肉
エキス、コーンステイープリカー等の天然窒素源の観：
二尿素等の有（幾窒索；原、ならびに硝酸す１リウム、
硝酸アンモニウム、硫酸アンモニウム等の無代窒素源を
用いることもでざる。Regarding nitrogen sources, natural nitrogen sources such as veg1, yeast extract, malt extract, meat extract, and corn staple liquor:
Presence of diurea, etc.
It is also not possible to use a free nitrogen source such as ammonium nitrate or ammonium sulfate.
この池必要に応し、リン酸塩、硫酸マグネシウム、硫酸
鉄、硫酸銅等の無磯塩及びビタミン等も微量栄養源とし
て使用できる。これらの培地成分は微生物の生育を害し
ない濃度であれば待に制限はない。Depending on the needs of the pond, mineral-free salts such as phosphate, magnesium sulfate, iron sulfate, copper sulfate, and vitamins can also be used as trace nutrients. There is no limit to the concentration of these medium components as long as they do not impair the growth of microorganisms.
実用上一般に炭素源は１へ２０重量％、窒素源はｏ４へ
ｚ重量％）濃度とする。培養温度は２０〜４０°Ｃとし
、培地のＰｌ＋は４〜１０として、通気攪拌、振盪又は
静置培養を行う。培養は通常２〜１０口問行う。In practice, the concentration of the carbon source is generally 1 to 20% by weight, and the nitrogen source is 0 to z% by weight. The culture temperature is 20 to 40°C, the Pl+ of the medium is 4 to 10, and aeration stirring, shaking, or static culture is performed. Culture is usually carried out for 2 to 10 times.
固体培地で培養する場合には６０−９０重量％の水を加
えたふすま、もみがら、本ぬが、麦芽粕等を用し・加熱
滅菌後２５〜４０°Ｃの温度において３−８０間培養を
行う。When culturing on a solid medium, use bran, rice husks, nuts, malt lees, etc. with 60-90% water added. After heat sterilization, culture at a temperature of 25-40°C for 3-80 days. I do.
このように培養１れば培地中にキシラナーゼが蓄積され
る。〕す後は培地り弓、酵素を抽出し、エタノール成る
いはアセ）ンなどによる溶媒沈澱法、硫安塩析法又は肝
膜処理法及びカラムクロマトグラフィーなどの常法に従
い、より精製された酵素剤を調製することができる。In this manner, xylanase is accumulated in the medium during culture 1. After that, the enzyme is extracted from the culture medium, and the enzyme is purified using conventional methods such as solvent precipitation with ethanol or acetone, ammonium sulfate salting out, liver membrane treatment, and column chromatography. An agent can be prepared.
犬に実施例によりこの発明をさらに具体的に説明するか
、これらに限定されるものではなす）。The present invention will be further illustrated by, but not limited to, examples for dogs.
なお、キシラナーゼ活性の測定法は、Ｏ，［３２５％の
ラーチウンドキシラン懸濁液２【Ｃ１ｇに酵素液０．５
＋ｉＲを加え、ＩＯｏＣ−３０＋ｎｉｎで反応後、生成
した還元糖をネルソン・ソモキ法で測定し、キシロース
として１分間に１μ＋１１Ｏｌ ｅの還元糖を生成する
場合を１単位とした。The method for measuring xylanase activity is as follows: O, [325% Larchound xylan suspension 2
After adding +iR and reacting with IOoC-30+nin, the produced reducing sugar was measured by the Nelson-Somoki method, and one unit was defined as the production of 1 μ+11 Ole of reducing sugar as xylose per minute.
本発明の好適な実施例を次項に説明する。A preferred embodiment of the invention is described in the following section.
（実施例１）
酵素剤の調製は以ｒのように行った。即ちケトミウム・
グラシル ｒ Ｆｏ ６５６８又はその変異株の胞子−
をボテ１デキストロース寒天斜面よりふすま＋３地（）
−Ｆ 主１０ Ｂ／ ５００ｍＲ’Ｊｌ角７う入ｓ、１
２０°Ｃ３０＋ｎｉｎ加熱滅菌）に接種し、３０’Ｃ、
トロ日間静置培養し、；）５°Ｃ温水で酵素を抽出して
常法の通りに工タノールによる分画沈澱法を以て酵素剤
を調製した。得られた酵素標品はそれぞれ（第１表）ｌ
こ示゛１通りであった。(Example 1) An enzyme agent was prepared as follows. That is, ketomium
Spores of Gracil r Fo 6568 or its mutant strain
Combine 1 dextrose agar slope with bran + 3 soil ()
-F Main 10 B/ 500mR'Jl angle 7 increments, 1
Inoculated at 20°C (heat sterilized for 30+min), 30'C,
After statically culturing for several days, the enzyme was extracted with 5°C warm water, and an enzyme preparation was prepared by fractional precipitation using ethanol in a conventional manner. Each of the enzyme preparations obtained (Table 1)
This was exactly the case.
（第１表） （注）変異株は原体の人工変異株（既述）である。(Table 1) (Note) The mutant strain is an artificial mutant strain of the original product (as described above).
（実施例２）
各種植物体の脱リグニン後、アルカリ抽出によって得ら
れた不溶性キシランを原料（第２表）として分解した場
合について実施しｒこ。(Example 2) This experiment was carried out in the case where insoluble xylan obtained by alkaline extraction after delignification of various plants was decomposed as a raw material (Table 2).
即ち５重量％のキシラン１刈濁液（ｒ’ｌ１４．５）
４ ＋ｎｅを基質とし、これに比較のために起源を異に
した市販のキシラナーゼ剤（第３表）をそれぞれ１０単
位ず一〕添加してＬ字型試験管でＳ５°Ｃ１４時間の振
盪反応を行い、高速液体クロマトグラフィーで生成糖を
測定した。i.e. 5% by weight xylan 1 suspension (r'l14.5)
4 +ne as a substrate, 10 units each of commercially available xylanase agents (Table 3) of different origins were added for comparison, and a shaking reaction was carried out at S5°C for 14 hours in an L-shaped test tube. The sugar produced was measured using high-performance liquid chromatography.
（第２表）
基質とした各種原料ヘシランの糖組成（％）（第３表）
供試酵素剤
］５
結果は第５図のように本発明の酵素剤ＣＧは他のキシラ
ナーゼ剤 ＴＸ、ＡＣＩ−１と比へて明らかにキシロビ
オースを多く生成した。(Table 2) Sugar composition (%) of various raw material hesylan used as a substrate (Table 3) Test enzyme agent] 5 The results are shown in Figure 5. The enzyme agent CG of the present invention was different from other xylanase agents TX, ACI. -1 clearly produced more xylobiose.
（実施例３）
本発明１こよる酵素剤は麦芽粕キシランの場合、１与１
こ効率よくキシロビオースを生成する１Ｓ徴を有してい
るか、この点をキシロビオース生成能かすぐれているこ
とがすでに報告されているバチルス６プミルス（］Ｔ３
ａｃｉｌｌｕｓ ｐｕｍｉｌｕｓ）由来お上び７ミコー
ラ・ラヌギノサ（ＩＩｕ＋ｆｌｉｃすｌａ １ａｎＢｉ
ｎｏｓａ）由来の酵素剤（第を表）とも比較してみた。(Example 3) In the case of malt cake xylan, the enzyme preparation according to the present invention 1 is 1 to 1
Bacillus 6 pumilus (]T3, which has been reported to have a 1S characteristic that efficiently produces xylobiose, or has an excellent ability to produce xylobiose in this respect.
acillus pumilus) origin 7 Mycola lanuginosa (IIu+flicsla 1anBi
A comparison was also made with enzyme preparations (see Table 1) derived from Nosa.
即ち本実施例はアルカリ抽出法によって調製した不溶性
の麦芽粕キシラン（ｔｉｆＪ２表）を基質とし、実施例
２と同様にして４時間の酵素反応を行った。That is, in this example, an enzyme reaction was carried out for 4 hours in the same manner as in Example 2 using insoluble malt meal xylan (Table tifJ2) prepared by the alkaline extraction method as a substrate.
（第４表）
皆集Ｐ、７１３ （特開昭６１−２４２５９２号公報
）木、 ＼’、ＫＩＴＰＩＩＥＥＣＨＡＶＡｔ４１Ｃ
Ｈ，Ｍ、トＩＡＹΔＳＨ１，Ｓ 、ＮΔＧＡＩ ；
Ｊ 、Ｆｅｒ＋ｎｅｎＬ、Ｔｅｃ −１＋ｎｏ１．＋６
２．６３．４１５（１，９８４）その結果は第６図に示
すように、本発明の酵素剤は彰貨および旧し由来の酵素
剤（こ比べても、不溶性麦芽粕キシランから若しく効率
よでキシロビオースを生成するキシラナーゼであること
が確認された。(Table 4) Collection P, 713 (Unexamined Japanese Patent Publication No. 61-242592) Tree, \', KITPIIEECHAVAt41C
H, M, IAYΔSH1,S, NΔGAI;
J, Fer+nenL, Tec-1+no1. +6
2.63.415 (1,984) As shown in FIG. 6, the results show that the enzyme preparation of the present invention is made from insoluble malt cake xylan and is less efficient than the enzyme preparations derived from Shoko and old ones. It was confirmed that this is a xylanase that produces xylobiose.
（実施例４）
本実施例ｔは、麦芽粕の高圧蒸煮（１０１、ｇ 、’
ｃ ＋＋＋ ：′０−１０＋ｏｉｎ保↑″ｔ）熱水（１
８４℃）抽出法によって調製した可溶性キシラン（糖組
成、キシロース３．１％、アラビノース１．１％、グル
コース１．２％）を分解する場合の酵素濃度の影響１こ
ついて調べたもので′ある。(Example 4) In this example t, high-pressure steaming of malt lees (101, g,'
c +++ :'0-10+oin ↑''t) Hot water (1
The effect of enzyme concentration on the decomposition of soluble xylan (sugar composition: 3.1% xylose, 1.1% arabinose, 1.2% glucose) prepared by the extraction method (84℃) was investigated. .
即ちＬ字型試ｇ、管中に可溶性キシラン４Ｉｒｌｅと酵
素剤の各呈を加え、実施例２と同様に酵素反応を行った
。That is, soluble xylan 4Irle and each enzyme agent were added to an L-shaped test tube, and an enzyme reaction was carried out in the same manner as in Example 2.
その結果は第７図に示したように、酵素剤添加量の増加
に件ってキシロビオース生成量が次第に上昇するが、キ
シロース、グルコースなとの単糖の生成量の伸びは者し
く少なく、本発明のキシラナーゼが麦芽粕の可溶性キシ
ランの分解によるキシロビオースの製造にも極めて有利
な＾Ｙ素剤であることが１′りっだ。As shown in Figure 7, the amount of xylobiose produced gradually increases as the amount of added enzyme agent increases, but the increase in the amount of monosaccharides such as xylose and glucose is clearly small; It is clear that the xylanase of the invention is an extremely advantageous base agent for the production of xylobiose by decomposing soluble xylan in malt meal.
（実施例５）
本実施例５は、ハーチウンドキシランを基質にしで、酵
素剤 ＣＧとＩ−Ｉ Ｌのキシラン分解限度を還元糖の
生成能を調べることによって比較したものて゛ある。即
も■−字型試験管にバーチウ、ｌ−゛へ−シラン０．０
２−１ ）ｉ、 Ｍ７’２０酢酸緩衝液（Ｐ Ｉ−１５
，５）３．８＋ｏｆｆ、 ｌＳｙ果液０．２＋ｎＲ（４
００単位）を入れ、モノド式振盪槻５５°Ｃで反応を開
始した。反応途中で酵素液０．］Ｊずつを数回添加し、
経時的に生成糖を測定した。(Example 5) In this Example 5, the xylan decomposition limits of the enzyme agents CG and I-IL were compared by examining the ability to produce reducing sugars using heartland xylan as a substrate. Immediately put birch in a --shaped test tube and add silane 0.0 to l-゛.
2-1)i, M7'20 acetate buffer (PI-15
,5) 3.8+off, lSy fruit juice 0.2+nR(4
00 units), and the reaction was started at 55°C using a Monod shaker. Enzyme solution 0.0 during the reaction. ] J several times,
The sugar produced was measured over time.
結果は第８図に示すように、本発明のキシラナーゼＣＧ
での分解（糖化）限度は土星でのそれよりも明らかに低
（、しかもその時点における糖組成は（ｆ５５表）の通
りであり、本発明のキシラナーゼ剤は分解（糖化）限度
に達−Ｙる迄の状態で使用しても、濃厚なキシロビオー
ス糖液を効率よく安定して製造し得ることが判った。The results are shown in FIG. 8, where xylanase CG of the present invention
The decomposition (saccharification) limit at Saturn is clearly lower than that at Saturn (and the sugar composition at that time is as shown in the f55 table), and the xylanase agent of the present invention reaches the decomposition (saccharification) limit -Y It was found that a concentrated xylobiose sugar solution could be efficiently and stably produced even when used in conditions up to
（第５表）
２〇−
（効果）
以上に詳しく説明したように、本発明によれば各種キシ
ランの酵素分解によってキシロオリゴ糖を製造する場合
、キシロースの生成が抑制される反面、特にキシロビオ
ースを効率よく生成させることができるため、その分取
精製が着しく容易になる利点がある。(Table 5) 20- (Effects) As explained in detail above, according to the present invention, when xylooligosaccharides are produced by enzymatic decomposition of various xylans, the production of xylose is suppressed, but especially xylobiose is efficiently Since it can be easily produced, it has the advantage that its preparative purification is simple and easy.
第１図は本発明酵素剤ＣＧの活性に及ぼす反応Ｐ Ｈの
影響を示すグラフ、第２図は活性に及ぼす反応温度の影
響を示すグラフ、第３図は酵素液の保存安定性に及ぼす
ＰＨの影響を示すグラフ、第４図は酵素液の加熱処理が
活性に及ぼす影響を示すグラフ、第５図は種々の酵素剤
で各種キシランを分解したときの、生成糖を比較したグ
ラフ、第６図は不溶性麦芽粕キシランの酵素分解におけ
る各種酵素剤の効果を比較したグラフ、第７図は本発明
酵素剤ＣＧによる可溶性麦芽粕キシランの酵素分解にお
ける生成糖の経緯を示したグラフ、第８図は匹及びＴ−
１１−にょるバーチランドキシランの糖化限度を比較し
たグラフである。
出願人 食品産業バイオリアクタ
■ ズ 紫 型 Ｉ
兵 ２ 紫 ぜ メ
堤
千Ｉ
卸
阜
口Ｄ
ヤ々
ヤ
や
ぜ
（’／、）↓軍玉
雰ｔ、口（＋ｆ−ａｉ妬
（ｏｌｏ） ＊穿壬
鱒、、Ｃ（１ｇＯ／；〜
第８図
バーチウ・・汁゛Ｎシラン（ＢＷＸ）の分角！（騎イ０
限度反応吟関
（ｈｒ）
改良時間
（ｈｒ）Figure 1 is a graph showing the effect of reaction PH on the activity of the enzyme agent CG of the present invention, Figure 2 is a graph showing the effect of reaction temperature on activity, and Figure 3 is a graph showing the effect of PH on the storage stability of the enzyme solution. Figure 4 is a graph showing the influence of heat treatment of enzyme solution on activity. Figure 5 is a graph comparing sugars produced when various xylans are decomposed with various enzymes. The figure is a graph comparing the effects of various enzymes on the enzymatic decomposition of insoluble malt meal xylan. Figure 7 is a graph showing the history of sugar produced in the enzymatic decomposition of soluble malt meal xylan using the enzyme agent CG of the present invention. Figure 8 and T-
11 is a graph comparing the saccharification limits of birchland xylan. Applicant: Food industry bioreactor■ Zu Murasaki Type I Soldier 2 Murasaki Ze Me Tsutsumi Sen I Wholesale Fuguchi D Yaya ya yaze ('/,)↓Guntama atmosphere t, mouth (+f-ai envy (olo) *pierced Trout, C (1gO/; ~ Figure 8 Birch... N Silane (BWX) angle of fraction! (Kii 0
Limit reaction test (hr) Improvement time (hr)
Claims (1)
シラナーゼ生産能を有する微生物を培養して酵素剤を調
製し、これを各種のキシランに作用させることによりキ
シロビオースを主体とするキシロオリゴ糖を生成させ、
その生成物からキシロビオースを採取することを特徴と
するキシロビオースの製造法。 ２）ケトミウム属の微生物がケトミウム・グラシル（Ｃ
ｈａｅｔｏｍｉｕｍｇｒａｃｉｌｅ）ＩＦＯ６５６８及
びその変異株であることを特徴とする特許請求の範囲第
１項記載のキシロビオースの製造法。[Scope of Claims] 1) A microorganism belonging to the genus Chaetomium and capable of producing xylanase is cultivated to prepare an enzyme preparation, and this is allowed to act on various types of xylan to produce xylo-oligosaccharides mainly consisting of xylobiose. generate,
A method for producing xylobiose, which comprises collecting xylobiose from the product. 2) Microorganisms of the genus Chaetomium are Chaetomium gracil (C
2. The method for producing xylobiose according to claim 1, characterized in that the xylobiose is produced using M. haetomium gracile) IFO6568 and its mutant strains.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63274785A JPH02119790A (en) | 1988-10-31 | 1988-10-31 | Production of xylobiose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63274785A JPH02119790A (en) | 1988-10-31 | 1988-10-31 | Production of xylobiose |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH02119790A true JPH02119790A (en) | 1990-05-07 |
Family
ID=17546528
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63274785A Pending JPH02119790A (en) | 1988-10-31 | 1988-10-31 | Production of xylobiose |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH02119790A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5660838A (en) * | 1990-05-31 | 1997-08-26 | Suntory Limited | Skin preparations for external use |
| US5922579A (en) * | 1995-12-18 | 1999-07-13 | Rohm Enzyme Finland Oy | Xylanases and uses thereof |
| US6228629B1 (en) | 1995-12-18 | 2001-05-08 | Röhn Enzyme Finland OY | Xylanases, genes encoding them, and uses thereof |
| US6635464B1 (en) | 1995-12-18 | 2003-10-21 | Rohm Enzyme Finland Oy | Xylanases, genes encoding them, and uses thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62155095A (en) * | 1985-12-27 | 1987-07-10 | Shokuhin Sangyo Baioriakutaa Syst Gijutsu Kenkyu Kumiai | Production of xylobiose |
-
1988
- 1988-10-31 JP JP63274785A patent/JPH02119790A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62155095A (en) * | 1985-12-27 | 1987-07-10 | Shokuhin Sangyo Baioriakutaa Syst Gijutsu Kenkyu Kumiai | Production of xylobiose |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5660838A (en) * | 1990-05-31 | 1997-08-26 | Suntory Limited | Skin preparations for external use |
| US5922579A (en) * | 1995-12-18 | 1999-07-13 | Rohm Enzyme Finland Oy | Xylanases and uses thereof |
| US6228629B1 (en) | 1995-12-18 | 2001-05-08 | Röhn Enzyme Finland OY | Xylanases, genes encoding them, and uses thereof |
| US6635464B1 (en) | 1995-12-18 | 2003-10-21 | Rohm Enzyme Finland Oy | Xylanases, genes encoding them, and uses thereof |
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