JPH01297175A - Water-repellent treatment of pipe nearby its tip end - Google Patents
Water-repellent treatment of pipe nearby its tip endInfo
- Publication number
- JPH01297175A JPH01297175A JP12654888A JP12654888A JPH01297175A JP H01297175 A JPH01297175 A JP H01297175A JP 12654888 A JP12654888 A JP 12654888A JP 12654888 A JP12654888 A JP 12654888A JP H01297175 A JPH01297175 A JP H01297175A
- Authority
- JP
- Japan
- Prior art keywords
- liquid
- tube
- members
- tip
- pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000005871 repellent Substances 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 56
- 238000000034 method Methods 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000004458 analytical method Methods 0.000 abstract description 19
- 229920002545 silicone oil Polymers 0.000 abstract description 7
- 238000004445 quantitative analysis Methods 0.000 abstract description 2
- 230000005660 hydrophilic surface Effects 0.000 abstract 1
- 229920001296 polysiloxane Polymers 0.000 abstract 1
- 239000000126 substance Substances 0.000 description 13
- 239000000523 sample Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- -1 felt Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000002940 repellent Effects 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 235000010724 Wisteria floribunda Nutrition 0.000 description 1
- PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 239000010775 animal oil Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000000994 depressogenic effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000002984 plastic foam Substances 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
Landscapes
- Application Of Or Painting With Fluid Materials (AREA)
- Coating Apparatus (AREA)
- Surface Treatment Of Glass (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
本発明は、管の先端等の表面を撥水性にするための方法
およびその方法の実施に用いる装置に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a method for making a surface such as a tip of a tube water repellent, and an apparatus used to carry out the method.
特に化学分析において一定量の試着液を供給するための
定量ピペットのノズル（吐出管）の先端付近に撥水性表
面を形成するのに有用な方法および装置に関する。In particular, the present invention relates to a method and apparatus useful for forming a water-repellent surface near the tip of a nozzle (discharge tube) of a metering pipette for supplying a fixed amount of sample liquid in chemical analysis.
［従来の技術］
最近、乾式法による臨床化学検査が、分析作業の簡易性
、測定の迅速性等において優れているため多く用いられ
るようになってきた。この乾式法による臨床化学検査は
、検体である血液等の液体試料中のグルコース、尿素窒
素（ＢＵＮ）等の特定成分と反応する試薬を含有させた
化学分析スライドに液体試料を点着し、試薬と特定成分
の反応により生じた発色、変色等を比色分析することに
より特定成分を定量している。[Prior Art] Recently, dry method clinical chemistry tests have come into widespread use because they are superior in terms of simplicity of analysis work, rapidity of measurement, and the like. In clinical chemistry testing using this dry method, a liquid sample is spotted on a chemical analysis slide containing a reagent that reacts with specific components such as glucose and urea nitrogen (BUN) in a liquid sample such as blood. Specific components are quantified by colorimetric analysis of color development, discoloration, etc. caused by the reaction of specific components.
従来、化学分析スライドに検体（試料液）を点着するに
は、ビペ・ソトで検体を所定量吸入しまた後、ピペット
の先端に検体の丸い液滴を形成し、この液滴を化学分析
スライドの中央部にそっと触れるようにして点着してい
た。Conventionally, in order to apply a specimen (sample solution) onto a chemical analysis slide, a predetermined amount of the specimen is inhaled using a pipette, and then a round droplet of the specimen is formed at the tip of the pipette, and this droplet is then applied to the chemical analysis slide. It was applied by gently touching the center of the analysis slide.
検体のこのような点着方法においては点着精度の個人差
が大きく、満足なｔ９１査精度を得ることが困難であっ
た。この個人差の主な原因は、ピペットの先端（ノズル
の〉と化学分析スライドの点着面との相対的な位置関係
を一定に保つことが困難なことと、液吸引後にノズルの
先端外側に付着している液を拭き取らないとしばしば液
滴が形成されないことにあった。In this method of spotting a specimen, there are large individual differences in spotting accuracy, making it difficult to obtain satisfactory t91 test accuracy. The main causes of this individual difference are that it is difficult to maintain a constant relative position between the tip of the pipette (nozzle) and the spotting surface of the chemical analysis slide, and that Droplets often did not form unless the adhering liquid was wiped off.
点着の個人差をなくすには、ピペットを架台に装着し、
手動でまたは０動的に液をピペットから吐出させるのが
、一つのよい方法である。しかしこの場合にはビベツ１
−の先端と化学分析スライドの点着面との距離が一定で
あるから、ピペットのノズルの先端に液滴を形成させて
から、化学分析スライドに接触させて点着することがで
きない。To eliminate individual differences in spotting, attach the pipette to a stand,
One good method is to force the liquid out of the pipette manually or dynamically. However, in this case, Bibetsu 1
Since the distance between the tip of the pipette and the spotting surface of the chemical analysis slide is constant, it is not possible to form a droplet at the tip of the pipette nozzle and then bring it into contact with the chemical analysis slide and deposit it.
化学分析スライドで検査される試料液には、全血、血漿
、血清、それらの希釈液、尿、唾液等種々あり、粘度等
が異なるので、化学分析スライドの液受各面（通常は展
開層）に吸収される速度が異なる。また、被検出成分に
よりそれぞれ異なる種類の分析スライドが用いられ、同
種の試料液でも化学分析スライドの種類によって液受各
面（通常は展開層）に吸収される速度が買なる。化学分
析スライドに吸収される速度に対し、とベットからの吐
出速度が速すぎると、液滴の一部がノズルの先端の周囲
に付着してそのまま残留するため、点着液量の誤差を生
ずる。すなわち、付着を起こした点着では供給液ｌが不
足となり、その後の点着では前に付着した液が合併する
ために、液量に正（＋）の誤差を生ずる。There are a variety of sample liquids tested on chemical analysis slides, such as whole blood, plasma, serum, their diluted solutions, urine, and saliva, which have different viscosities. ) are absorbed at different rates. Furthermore, different types of analysis slides are used depending on the component to be detected, and even for the same type of sample liquid, the speed at which it is absorbed into each surface of the liquid receiver (usually the developing layer) depends on the type of chemical analysis slide. If the speed at which the droplet is ejected from the bed is too fast compared to the speed at which it is absorbed into the chemical analysis slide, some of the droplets will adhere to the area around the nozzle tip and remain there, causing an error in the amount of liquid deposited. . That is, the supply liquid 1 is insufficient in the spotting that caused the adhesion, and in the subsequent spotting, the previously deposited liquid is combined, resulting in a positive (+) error in the liquid amount.
ノズル先端付近の管の外（ｌＩ！Ｉ表面に液滴が付着し
ないようにするために、ノズルの外側のみを撥水性の材
料で構成することも考えられた。し、かしノズルを二重
構造にすることは、ノズルの製造工程を複雑にし、コス
トを大きくする。In order to prevent droplets from adhering to the surface of the tube near the tip of the nozzle, it was also considered to construct only the outside of the nozzle with a water-repellent material. This structure complicates the nozzle manufacturing process and increases cost.
また液吸引の都度ノズルの先端外側に付着している液を
拭き取ることは手間がかかることであり、充分注意しな
いとつい忘れることがあり、液量の誤差を生ずる。Moreover, it is time-consuming to wipe off the liquid adhering to the outside of the tip of the nozzle every time liquid is sucked, and if you are not careful enough, you may forget to wipe it off, resulting in an error in the amount of liquid.
［解決しようとする技術的課題］
本発明は、分析において用いるピペット等のノズル（吐
出管）等の開放された管の先端から一定量の液を、−回
または反復して排出する際に、管の先端付近の外側に液
の一部が付着することを防ぐために、管の先端付近の外
側表面に撥水性表面を形成する方法、およびその方法の
実施に用いる装置を提供することである。[Technical Problems to be Solved] The present invention provides a method for discharging a certain amount of liquid from the tip of an open tube such as a nozzle (discharge tube) of a pipette used in analysis - times or repeatedly. An object of the present invention is to provide a method for forming a water-repellent surface on an outer surface near the tip of a tube in order to prevent a portion of liquid from adhering to the outside near the tip of the tube, and an apparatus for carrying out the method.
本発明はまた、管の先端付近の外側に液の一部が付着す
ることなく、試料液を高い液量幇間で一回または反復し
て滴下できるように、定量ピペットのノズルの先端付近
の外側表面に撥水性表面を形成する方法を提供すること
を、技術的課題とする
［技術的課題の解決手段］
本発明の上記課題の一つは、液保持性の面を有する２つ
の部材の、液保持性の面に撥水性を付与する性質をもつ
液を保持させ、前記２つの部材の液保持性の面に管の先
端付近を接触させて、管の先端付近の外面に撥水性表面
を形成する方法によって解決された。The present invention also provides a solution to the outside of the nozzle of the metering pipette so that a sample solution can be dropped once or repeatedly in a high volume interval without some of the liquid adhering to the outside of the tube near the tip. A technical problem is to provide a method for forming a water-repellent surface on a surface [Means for solving the technical problem] One of the above-mentioned problems of the present invention is to provide a method for forming a water-repellent surface on a surface of two members having liquid-retaining surfaces. A liquid having a property of imparting water repellency is retained on the liquid-retaining surface, and the vicinity of the tip of the tube is brought into contact with the liquid-retaining surface of the two members to form a water-repellent surface on the outer surface near the tip of the tube. Solved by the method of forming.
本発明の上記課題の他の一つは、液保持性である面を有
し、この液保持性の面に撥水性を付４する性質をもつ液
を保持している２つの部材と、管の先端付近を前記２つ
の部材の液保持性の面に同時に接触させつつ管がこれら
２つの面の間で回転するように管を保持する手段と、前
記２つの部材の液保持性の面が面に沿って互いに反対方
向に移動するように前記２つの部材を運動させる手段と
を備えることを特徴とする、管の先端付近の外面に撥水
性表面を形成する装置により、解決された。Another object of the present invention is to provide two members having a liquid-retaining surface and retaining a liquid having a property of imparting water repellency to the liquid-retaining surface, and a pipe. means for holding the tube such that the tube rotates between these two surfaces while simultaneously bringing the vicinity of the tip of the tube into contact with the liquid-retaining surfaces of the two members; and means for moving said two members so as to move in opposite directions along a surface.
本発明で虎水性処理さ７する管は、断面が長円、多角形
等の断面を有してもよいが、円であることが望ましい、
処理される管（例えばピペットのノズル）の外径は長さ
方向に対し一定でもよく、また変化してもよいが、管は
少なくとも先端付近で直線の回転体であることが好まし
い、管の外径が一定である場合には、先端部を除き円柱
の外周部に液保持性の面を設けることが好ましい、管の
先端付近が円錐台の形状をもつ場合は、先端を除き円錐
台の外周部に液保持性の面を設けることが好ましい。The pipe to be subjected to water-based treatment in the present invention may have an oval, polygonal, etc. cross section, but is preferably circular.
The outer diameter of the tube to be treated (e.g. the nozzle of a pipette) may be constant or variable along its length, but it is preferable that the tube is a linear rotating body at least near the tip. If the diameter is constant, it is preferable to provide a liquid-retentive surface on the outer periphery of the cylinder, excluding the tip. If the tube has a truncated conical shape near the tip, the outer periphery of the truncated cone, excluding the tip, is preferable. It is preferable to provide a liquid-retaining surface on the part.
２つの部材に液保持性の面を設けるには、部材の外面に
布類、フェルト、紙、不織布、紐類、糸等を巻き付ける
か、貼り付ければよい、液保持部材は柔らかい方がよい
。なぜなら管の先端付近を接したとき、少しへこむので
、管の外周の比較的広い面積に接するからである９部材
の全体あるいは中心付近以外の部分を液保持性材料で構
成してもよい。To provide a liquid-retaining surface on two members, cloth, felt, paper, nonwoven fabric, string, thread, etc. may be wrapped or attached to the outer surfaces of the members. It is preferable that the liquid-retaining member is soft. This is because when the tip of the tube comes into contact with the tube, it is slightly depressed and comes into contact with a relatively wide area of the outer periphery of the tube.The entirety of the nine members or the portions other than the center may be made of a liquid-retaining material.
本発明の方法の実施に際し、管の先端付近を前記２つの
部材の液保持性の面に同時に接触させ、該２つの面の間
で管を回転させる方法が好ましい９２つの部材の液保持
性の面はともに平面であってもよく、ともに円柱面また
は円柱面の一部であってもよい、前記２つの部材の一つ
が有する液保持性の面は平面で、他の】っが有する液保
持性の而は円柱面または円柱面の一部であってもよい。When carrying out the method of the present invention, it is preferable to bring the vicinity of the tip of the tube into contact with the liquid-retentive surfaces of the two members at the same time, and rotate the tube between the two surfaces. The surfaces may both be flat, or both may be cylindrical surfaces or parts of cylindrical surfaces.The liquid-retaining surface of one of the two members is flat, and the liquid-retaining surface of the other member is flat. The object may be a cylindrical surface or a part of a cylindrical surface.
前記２つの部材が互いに反対方向に平行に移動し得る液
保持性の平面を有するものであると、それらの面の間で
管の先端付近を回転させるに好都合である０例えば２つ
の部材が、対向する面に液保持性の平面を有する板状部
材であると、それらを互いに反対方向に平行に移動させ
ることにより、２つの平面の間に管の先端付近をはさん
で回転させることができ、好都合である。If the two members have liquid-retaining planes that can move parallel to each other in opposite directions, it is convenient to rotate the vicinity of the tip of the tube between those planes.For example, the two members: If the plate member has liquid-retaining flat surfaces on opposing surfaces, by moving them parallel to each other in opposite directions, it is possible to rotate the tube with the tip of the tube sandwiched between the two flat surfaces. , which is convenient.
前記２つの部材を円柱決たは円筒とすると、それらを互
いに同じ方向に回転させることによって、それらの外周
面の間に管の先端付近をはさんで、回転させるのに好都
合である。When the two members are cylindrical or cylindrical, by rotating them in the same direction, it is convenient to sandwich the tip of the tube between their outer peripheral surfaces and rotate the tube.
管の先端付近に撥水性表面を形成するために用いる撥水
性液体としては、抗水処理剤として広く用いられている
シリコン油が、入手のし易さ、価格等の点で好適である
。しかし、植物油、動物油、鉱油、合成エステル類、高
級アルコール等の中から管の先端付近の外周面に付着し
得るものを選んで用いてもよい、１！ｉ当な揮発性を持
つ溶媒を含んでもよい。As the water-repellent liquid used to form a water-repellent surface near the tip of the tube, silicone oil, which is widely used as an anti-water treatment agent, is suitable in terms of availability, price, etc. However, it is also possible to select from among vegetable oils, animal oils, mineral oils, synthetic esters, higher alcohols, etc., those that can adhere to the outer peripheral surface near the tip of the tube.1! It may also contain a solvent with appropriate volatility.
本発明は臨床化学検査において、試料液、試薬等の計量
に用いられるピペットの注液ノズルの先端外面の抗水化
処理に有用である。INDUSTRIAL APPLICABILITY The present invention is useful for anti-hydration treatment of the outer surface of the tip of a liquid injection nozzle of a pipette used for measuring sample liquids, reagents, etc. in clinical chemistry tests.
臨床化学検査における化学分析スライドへの点着等に用
いられるピペットは、シリンダーとピストンを有するも
のが多い６ビベツトは、ノズルが固定されているもので
もよいし、着脱自在な、またさらに使い捨てのノズルチ
ップを用いるものでもよい、ノズルチップは例えば米国
特許 ４，０７２゜３３０、同４，２３７，０９５、同
４，３４７，８７５に記載されたものを用いることがで
きる。市販のノズルチップは、加工精度の点からポリプ
ロピレン製のものが多い。Pipettes used for dotting on chemical analysis slides in clinical chemistry tests often have a cylinder and a piston.The 6-bib pipette may have a fixed nozzle, or may be a removable or disposable nozzle. A nozzle tip may be used, for example, those described in US Pat. No. 4,072.330, US Pat. No. 4,237,095, and US Pat. Many commercially available nozzle tips are made of polypropylene from the viewpoint of processing accuracy.
ピペットのノズルの先端の外径は、通常０．３ｍｍから
１．５１程度のものである。好ましくは０．５ｍｍから
１，２１である。The outer diameter of the tip of the pipette nozzle is usually about 0.3 mm to 1.51 mm. Preferably it is from 0.5 mm to 1.21 mm.
本発明は管の外径により適用が制限されるものではない
が、液滴が管の外面に形成されるような場合に有用であ
り、５Ｍｌ１１をこえる外径をもつ管では実際的な効果
は得にくいであろう。Although the present invention is not limited in its application by the outer diameter of the tube, it is useful in cases where droplets are formed on the outer surface of the tube, and has no practical effect on tubes with an outer diameter exceeding 5 Ml11. It will be difficult to obtain.
着脱自在のノズルを有するピペット等に適用する場合、
ノズルの長さは特（１こ制限はないが、本発明を実施す
るに際してノズルは保持することができるだけの長さを
もつ必要がある。使い捨てのノズルチップ等の場合は、
収容すべき所望の液量によってノズルの長さは自ずから
きまる。本発明によって先端付近の外面を撥水性にする
範囲は、外径が０．５ないし３１程度のノズルの場合、
先端から１０ｍｍ程度までで充分で、３ないし１．０＋
ｎ＋＋＋程度が最適である。先端からこれ以上遠いとこ
ろまで（発水性にすると、外面に沿う液の流れ落ちを防
止する効果がむしろ低下する。When applied to pipettes etc. with removable nozzles,
The length of the nozzle is 1 (there is no particular limit, but the nozzle must be long enough to be held when carrying out the present invention. In the case of a disposable nozzle tip, etc.
The length of the nozzle is determined by the desired amount of liquid to be accommodated. In the case of a nozzle with an outer diameter of about 0.5 to 31, the range in which the outer surface near the tip is made water repellent by the present invention is:
Approximately 10mm from the tip is sufficient, 3 to 1.0+
The optimum value is about n+++. If it is made water-repellent further away from the tip, the effectiveness of preventing the liquid from flowing down along the outer surface will actually decrease.
［発明の効果］
本発明を用いると、分析において用いるピペット等のノ
ズル（吐出管）等の開放された管の先端から一定量の液
を、−回または反復して排出する際に、管の先端付近の
外側に液の一部が付着することがないので、管から滴下
される液量の正または負の誤差が生じない、その結果、
例えば液中の成分に関する定量化学分析の精度を向上す
ることができる７
また本発明を用いると、液を吸入するためにピペットの
ノズル部を比較的深く液中に浸けたときに、ノズル部の
比較的上方の外面に付着した液が徐々に流れ落ちてきて
、排出される液に加わること（滴下液量の正誤差となる
）も防止できる。[Effect of the invention] When the present invention is used, when a certain amount of liquid is discharged from the tip of an open tube such as a nozzle (discharge tube) of a pipette used in analysis - times or repeatedly, Since no part of the liquid adheres to the outside near the tip, there is no positive or negative error in the amount of liquid dripped from the tube.As a result,
For example, the accuracy of quantitative chemical analysis regarding components in a liquid can be improved. It is also possible to prevent the liquid adhering to the relatively upper outer surface from gradually flowing down and being added to the liquid being discharged (causing an error in the amount of dropped liquid).
本発明は、ピペットに限らず、定量分析に用いるビュー
レット等にも有用である。さらに本発明は、化学分析に
用いる液体計量容器のみならず、比較的−・定した大き
さの液滴を常に形成することが重要な場合に有用である
（例えば点滴の際のように滴数により流量を管理する場
合）。The present invention is useful not only for pipettes but also for burettes and the like used for quantitative analysis. Furthermore, the present invention is useful not only in liquid measuring containers used in chemical analysis, but also in cases where it is important to consistently form droplets of a relatively constant size (e.g., during infusion, the number of droplets (when controlling the flow rate).
［実施例１］
第１図に示すような装置を製作した。第１図において、
１は支持枠、２および２″は樋状部材、３および３′は
樋状部材２および２′にそれぞれ固定された液保持部材
、４および４′は樋状部材の−・方の縁に設けられたラ
ック、５はラック４，４゛に噛み合うビニオ〉・、６お
よび６″は板状部材２゜２°を互いに反対の方向に付勢
するバネ、７は処理される管、８は管保持部材、９はバ
ネ６の付勢に抗して板状部材２を水平方向に運動させる
ノブを示す、液保持部材３は樋状部材２の縁にはさまれ
た面に、液保持部材３゛は樋状部材２“の縁にはさまれ
た面に、それぞれ固定されている。液保持部材３と３′
は、管保持部材に保持された管７に接することができる
。管保持部材８は中心に、管７を通して回転自在に保持
するための穴を有する。[Example 1] A device as shown in FIG. 1 was manufactured. In Figure 1,
1 is a support frame, 2 and 2'' are gutter-like members, 3 and 3' are liquid retaining members fixed to gutter-like members 2 and 2', respectively, and 4 and 4' are on the - edge of the gutter-like members. 5 is a rack that engages with the racks 4 and 4'', 6 and 6'' are springs that bias the plate members 2° and 2° in opposite directions, 7 is a pipe to be processed, and 8 is a The tube holding member 9 indicates a knob that moves the plate member 2 in the horizontal direction against the bias of the spring 6. The members 3'' are fixed to the surfaces sandwiched between the edges of the gutter-like member 2''.The liquid retaining members 3 and 3'
can be in contact with the tube 7 held by the tube holding member. The tube holding member 8 has a hole in the center for rotatably holding the tube 7 therethrough.
液保持部材３および３°はフェルトである。The liquid retaining members 3 and 3° are felt.
液保持部材３および３°の全面に適量の市販シリコン油
を浸み込ませ、管保持部材８の穴に処理すべき管７、例
えばマイクロピペットのノズルデツプを下向きに差し込
む、ノブ９を指で押すと、樋状部材２がバネ６の付勢に
抗して図中右向きに動かされ、同時にラック４が図中右
向きに動き、これと噛み合ったビニオン５を介してラッ
ク４′が図中左向きに動かされる。ラック４゛と一体で
ある樋状部材２°は従って樋状部材２と反対方向に運動
する。これにより樋状部材２および２′にはさまれた管
７は時計方向に回転する。Fill the entire surface of the liquid holding member 3 and 3° with an appropriate amount of commercially available silicone oil, insert the tube 7 to be processed, for example, the nozzle depth of a micropipette, downward into the hole of the tube holding member 8, and press the knob 9 with your finger. Then, the gutter-like member 2 is moved to the right in the figure against the bias of the spring 6, and at the same time, the rack 4 is moved to the right in the figure, and the rack 4' is moved to the left in the figure via the pinion 5 that meshes with the rack 4. Moved. The trough 2°, which is integral with the rack 4', therefore moves in the opposite direction to the trough 2. This causes the tube 7 sandwiched between the trough-like members 2 and 2' to rotate clockwise.
管７の先端は液保持部材３および３゛に接して回転する
ので、管５の先端部が液保持部材３および３′に接する
面は、上記回転の間に管７の全周に及ぶ、すなわち管７
の先端付近の全周にシリコン油が付着し、撓水性の表面
が形成される。このようにして、管７の先端付近の外面
に撥水性の表面が形成された。Since the tip of the tube 7 rotates in contact with the liquid holding members 3 and 3', the surface where the tip of the tube 5 contacts the liquid holding members 3 and 3' extends over the entire circumference of the tube 7 during the rotation. i.e. tube 7
Silicone oil adheres to the entire circumference near the tip, forming a water-repellent surface. In this way, a water-repellent surface was formed on the outer surface near the tip of the tube 7.
［実施例２］
第２図に示すような装置を製作した。第２図において、
１は基板を兼ねる外箱、２および２′は円柱状部材、３
および３′は円柱状部材を囲んで設けられた液保持部材
、４および４′は円柱状部材２および２゛にそれぞれ固
定された歯車、５は軸５ａの回りに回転する回動部材、
６は回動部材５を回転さぜるための歯車群、７は処理さ
れる管、８は管保持部材、８ａは管保持部材８の一端に
設けられ歯車４および４゛と噛み合う固定歯車、９はモ
ータを示す。円柱状部材２および２′は回動部材５に固
定された軸２ａおよび２ａ’の回りに回転できる。円柱
状部材２，２′の面に設けられた液保持部材３，３°は
、管保持部材８に保持された管７に接することができる
。管保持部材８は中心に管７を通して菌持するだめの穴
を有し、外箱１に固定されている。管７は保持部材８に
より回転自在に保持される。液保持部材３．３°はスポ
ンジ（発泡プラスチック）である６モータ９は、図示し
ないスイッチを介し、図示しない電源に接続されている
。[Example 2] A device as shown in FIG. 2 was manufactured. In Figure 2,
1 is an outer box that also serves as a substrate, 2 and 2' are cylindrical members, and 3
and 3' is a liquid holding member provided surrounding the cylindrical member; 4 and 4' are gears fixed to the cylindrical members 2 and 2', respectively; 5 is a rotating member that rotates around the shaft 5a;
6 is a group of gears for rotating the rotating member 5; 7 is a tube to be processed; 8 is a tube holding member; 8a is a fixed gear provided at one end of the tube holding member 8 and meshing with gears 4 and 4'; 9 indicates a motor. The cylindrical members 2 and 2' can rotate around axes 2a and 2a' fixed to the pivot member 5. The liquid retaining members 3 and 3° provided on the surfaces of the cylindrical members 2 and 2' can be in contact with the tube 7 held by the tube retaining member 8. The tube holding member 8 has a hole in the center for passing the tube 7 through and retaining bacteria, and is fixed to the outer box 1. The tube 7 is rotatably held by a holding member 8. The liquid holding member 3.3° is made of sponge (plastic foam) 6. The motor 9 is connected to a power source (not shown) via a switch (not shown).
モータ９が停止した状態で、液保持部材３および３′に
市販シリコン油を供給して浸み込ませ、管保持部材８の
穴に処理すべき管７、例えばマイクロとベットのノズル
チップを下向きに差し込む。With the motor 9 stopped, commercially available silicone oil is supplied to the liquid holding members 3 and 3', and the nozzle tip of the tube 7 to be processed, for example, a micro and bed, is inserted into the hole of the tube holding member 8, facing downward. Insert it into the
モータ９を回転させると、歯車群６を介して回動部材５
が軸５ａの回りに回転し、歯車４，４′が歯車８ａの回
りに公転しながら軸２ａの回りに自転する。ｔＪｉ車４
．４゛が固定された円柱状部材２゜２゛、従って液保持
部材３，３“も同じような回転運動をする。管７の先端
は液保持部材３と３°の間にはさまれて回転するので、
管７の先端部が液保持部材３および３′に接する面は、
上記回転の間に管７の全周に及ぶ、すなわち管７の先端
付近の全周にシリコン油が付着し、撥水性の表面が形成
される。このようにして、管７の先端付近の外面に撥水
性の表面が形成された。When the motor 9 is rotated, the rotating member 5 is rotated through the gear group 6.
rotates around the shaft 5a, and the gears 4 and 4' rotate around the shaft 2a while revolving around the gear 8a. tJi car 4
．． The cylindrical member 2゜2゛ to which 4゛ is fixed, and therefore the liquid holding members 3 and 3'' also undergo a similar rotational movement.The tip of the tube 7 is sandwiched between the liquid holding member 3 and 3°. Because it rotates,
The surface where the tip of the tube 7 contacts the liquid holding members 3 and 3' is
During the above rotation, silicone oil adheres to the entire circumference of the tube 7, that is, to the entire circumference near the tip of the tube 7, forming a water-repellent surface. In this way, a water-repellent surface was formed on the outer surface near the tip of the tube 7.
第１図は本発明の実施例１に用いた装置の使用状態を示
す３方向の断面図、第２図は本発明の実施例２に用いた
装置の使用状官を示す断面図、第３図は本発明の方法で
処理されたノズルチップに形成される液滴の状態を示す
略図、第４図は外周に撥水性表面を形成していないノズ
ルチップ（従来技術）に形成される液滴の状態を示す略
図である。
出願人 富士写真フィルム株式会社第２図
第３図
平糸ん打１］「トイｌＴ（自発）
＼Ｖ成１年４月３［１
１、１１件の表示
昭和６３年特許願第１２６５４８号
２ 発明の名称
管の先端付近のｆｈｘ性処理
３、補正をする者
’ＪＴ件との関係 特許出願人
住 所 神奈川県南足柄市中沼２１０番地富士写工１
フィルム株式会社 東京本社電話（４０６）２５３７
４、補正の対象 明細書の「発明の詳細な説明Ｊの欄
５、補正の内容
明細書の発明の詳細な説明の欄の記載を以下の通り補正
する。
明細書第１０ページ１行目の「でもよい。」の後に［前
記シリコン油は、分子量５０００以−Ｌのものを用いる
のが好ましい。」を挿入する。
以上FIG. 1 is a cross-sectional view in three directions showing how the device used in Example 1 of the present invention is used, FIG. 2 is a cross-sectional view showing the state of use of the device used in Example 2 of the present invention, and FIG. The figure is a schematic diagram showing the state of droplets formed on a nozzle chip treated by the method of the present invention, and Figure 4 shows droplets formed on a nozzle chip (prior art) that does not have a water-repellent surface formed on its outer periphery. It is a schematic diagram showing the state of. Applicant: Fuji Photo Film Co., Ltd. Figure 2 Figure 3 Hiraitonuchi 1] "Toy IT (Spontaneous) \V April 3, 1989 [1 1, 11 Displays 1988 Patent Application No. 126548 2 Invention Name of fhx processing near the tip of the tube 3, person making the correction Relationship with the JT case Patent applicant address Fujishako 1, 210 Nakanuma, Minamiashigara City, Kanagawa Prefecture
Film Co., Ltd. Tokyo Head Office Tel: (406) 2537 4. Subject of amendment The description in the "Detailed Description of the Invention J" column 5 of the description and the "Detailed Description of the Invention" column of the description of the amendment will be amended as follows. After "may be." in the first line of page 10 of the specification, it is preferable that the silicone oil has a molecular weight of 5000-L or more. ” is inserted. that's all
Claims (1)
の面に撥水性を付与する性質をもつ液を保持させ、前記
２つの部材の液保持性の面に管の先端付近を接触させて
、管の先端付近の外面に撥水性表面を形成する方法。 ２）前記液保持性の面が柔軟である、特許請求の範囲１
）の方法。 ３）管の先端付近を前記２つの部材の液保持性の面に同
時に接触させ、該２つの面の間で管を回転させる、特許
請求の範囲１）の方法。 ４）前記２つの部材の液保持性の面がともに平面である
、特許請求の範囲１）の方法。 ５）前記２つの部材の一つが有する液保持性の面は平面
であり、他の一つが有する液保持性の面は円柱面または
円柱面の一部である、特許請求の範囲１）の方法。 ６）前記２つの部材の液保持性の面が円柱面または円柱
面の一部である、特許請求の範囲１）の方法。 ７）前記２つの部材が円柱または円筒である、特許請求
の範囲１）の方法。 ８）液保持性である面を有し該液保持性の面に撥水性を
付与する性質をもつ液を保持している２つの部材と、管
の先端付近を前記２つの部材の液保持性の面に同時に接
触させつつ管が該２つの面の間で回転するように保持す
る手段と、前記２つの部材の液保持性の面が面に沿って
互いに反対方向に移動するように前記２つの部材を運動
させる手段とを備えることを特徴とする、管の先端付近
の外面に撥水性表面を形成する装置。 ９）前記２つの部材が互いに反対方向に平行に移動し得
る液保持性の平面を有する、特許請求の範囲８）の装置
。 １０）前記２つの部材が回転し得る円柱または円筒であ
る、特許請求の範囲８）の装置。[Scope of Claims] 1) The liquid-retaining surfaces of two members having liquid-retaining surfaces retain a liquid having a property of imparting water repellency, and the liquid-retaining surfaces of the two members A method of forming a water-repellent surface on the outer surface near the tip of the tube by bringing the tip of the tube into contact with the tube. 2) Claim 1, wherein the liquid-retaining surface is flexible.
)the method of. 3) The method according to claim 1, wherein the vicinity of the tip of the tube is brought into contact with liquid-retaining surfaces of the two members at the same time, and the tube is rotated between the two surfaces. 4) The method according to claim 1), wherein both liquid-retaining surfaces of the two members are flat. 5) The method according to claim 1), wherein the liquid-retaining surface of one of the two members is a flat surface, and the liquid-retaining surface of the other member is a cylindrical surface or a part of a cylindrical surface. . 6) The method according to claim 1, wherein the liquid-retentive surfaces of the two members are cylindrical surfaces or portions of cylindrical surfaces. 7) The method of claim 1), wherein the two members are cylinders or cylinders. 8) Two members that hold a liquid that has a liquid-retaining surface and have a property of imparting water repellency to the liquid-retaining surface, and the liquid-retaining surface of the two members near the tip of the tube. means for holding the tube so that it rotates between the two surfaces while being simultaneously in contact with the surfaces of the two members; A device for forming a water-repellent surface on the outer surface of a tube near its tip, characterized in that it comprises means for moving two members. 9) The device according to claim 8, wherein the two members have liquid-retentive planes that are movable parallel to each other in opposite directions. 10) The device of claim 8), wherein the two members are rotatable cylinders or cylinders.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12654888A JPH0829292B2 (en) | 1988-05-24 | 1988-05-24 | Water treatment near the tip of the pipe |
| DE68920067T DE68920067T2 (en) | 1988-04-28 | 1989-04-26 | Method and device for imparting water-repellent properties to a tubular element. |
| EP89107584A EP0339622B1 (en) | 1988-04-28 | 1989-04-26 | Method and apparatus for water-repellent processing of tube |
| US07/343,314 US5051282A (en) | 1988-04-28 | 1989-04-26 | Method and apparatus for water-repellent processing of tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12654888A JPH0829292B2 (en) | 1988-05-24 | 1988-05-24 | Water treatment near the tip of the pipe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01297175A true JPH01297175A (en) | 1989-11-30 |
| JPH0829292B2 JPH0829292B2 (en) | 1996-03-27 |
Family
ID=14937903
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12654888A Expired - Fee Related JPH0829292B2 (en) | 1988-04-28 | 1988-05-24 | Water treatment near the tip of the pipe |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0829292B2 (en) |
-
1988
- 1988-05-24 JP JP12654888A patent/JPH0829292B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0829292B2 (en) | 1996-03-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US3368872A (en) | Automatic chemical analyzer | |
| US3260413A (en) | Automatic chemical analyzer | |
| US3952599A (en) | Fractional-fill capillary pipette and method | |
| US3768978A (en) | Disposable pipette | |
| US4340390A (en) | Method and apparatus for metering biological fluids | |
| US4452899A (en) | Method for metering biological fluids | |
| US3216804A (en) | Automatic chemical analyzer and sample dispenser | |
| CN101990631A (en) | Sample acquisition device | |
| JPH1137922A (en) | Measurement chip for optical analyzer | |
| EP1637881B1 (en) | Sample arraying/assembling device, its method, and apparatus using sample assembly | |
| CA2040920C (en) | Capillary inoculator and assembly for inoculating multiple test sites and method of inoculation test sites therewith | |
| RU2290259C2 (en) | Method and the device for the biopolymer fields production | |
| JPH01274866A (en) | Treatment for imparting water repellency to pipe tip | |
| JP2000088863A (en) | Dispensing needle body for microdispenser | |
| JP4786087B2 (en) | Dispensing device for liquid to be dispensed | |
| JPH01297175A (en) | Water-repellent treatment of pipe nearby its tip end | |
| US5051282A (en) | Method and apparatus for water-repellent processing of tube | |
| JPH01317548A (en) | Water repellent treatment near pipe end | |
| JPH01274847A (en) | Water-repellent treatment of tube | |
| JPH11337557A (en) | Micro dispenser device | |
| JP4449977B2 (en) | Liquid dispensing and liquid printing equipment | |
| AU770194B2 (en) | Methods for providing liquid and solid components of a sample for use in assay methods | |
| CN108717009B (en) | Smear device and application and use method thereof in immunohistochemical test | |
| JP3069211B2 (en) | Biochemical analysis method | |
| JPH0431739B2 (en) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313111 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20080327 Year of fee payment: 12 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| LAPS | Cancellation because of no payment of annual fees |