JPH01269430A - Method and propagating shoot of plant belonging to genus rosa - Google Patents
Method and propagating shoot of plant belonging to genus rosaInfo
- Publication number
- JPH01269430A JPH01269430A JP63098358A JP9835888A JPH01269430A JP H01269430 A JPH01269430 A JP H01269430A JP 63098358 A JP63098358 A JP 63098358A JP 9835888 A JP9835888 A JP 9835888A JP H01269430 A JPH01269430 A JP H01269430A
- Authority
- JP
- Japan
- Prior art keywords
- medium
- genus rosa
- plants
- lateral buds
- seedlings
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000011449 Rosa Nutrition 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims description 13
- 230000001902 propagating effect Effects 0.000 title claims description 7
- 238000005273 aeration Methods 0.000 claims abstract description 12
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000004062 cytokinin Substances 0.000 claims abstract description 10
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims abstract 2
- 238000012258 culturing Methods 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 11
- 241000196324 Embryophyta Species 0.000 abstract description 25
- 239000001963 growth medium Substances 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 241000123589 Dipsacus Species 0.000 abstract 1
- 238000009630 liquid culture Methods 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 32
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 12
- 229910052760 oxygen Inorganic materials 0.000 description 12
- 239000001301 oxygen Substances 0.000 description 12
- 239000007789 gas Substances 0.000 description 7
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 6
- 229940088594 vitamin Drugs 0.000 description 6
- 229930003231 vitamin Natural products 0.000 description 6
- 235000013343 vitamin Nutrition 0.000 description 6
- 239000011782 vitamin Substances 0.000 description 6
- 229930024421 Adenine Natural products 0.000 description 5
- 229960000643 adenine Drugs 0.000 description 5
- 239000003375 plant hormone Substances 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 241000220317 Rosa Species 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 235000010295 Rosa x kordesii Nutrition 0.000 description 3
- 235000004789 Rosa xanthina Nutrition 0.000 description 3
- 241000109329 Rosa xanthina Species 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- -1 pentylaminopurine 2-naphthylmethylaminopurine Chemical compound 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
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- 241000967859 Rosa setigera Species 0.000 description 2
- 235000003609 Rosa setigera var setigera Nutrition 0.000 description 2
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- 235000011725 climbing rose Nutrition 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
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- JKQOBWVOAYFWKG-UHFFFAOYSA-N molybdenum trioxide Chemical compound O=[Mo](=O)=O JKQOBWVOAYFWKG-UHFFFAOYSA-N 0.000 description 2
- 238000004161 plant tissue culture Methods 0.000 description 2
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- 235000008160 pyridoxine Nutrition 0.000 description 2
- 239000011677 pyridoxine Substances 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 229940011671 vitamin b6 Drugs 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
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- 229930191978 Gibberellin Natural products 0.000 description 1
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- 239000004471 Glycine Substances 0.000 description 1
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- 240000005979 Hordeum vulgare Species 0.000 description 1
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- 206010021033 Hypomenorrhoea Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
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- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
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- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
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- 229930003268 Vitamin C Natural products 0.000 description 1
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- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 description 1
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- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
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- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 description 1
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- 239000010941 cobalt Substances 0.000 description 1
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- 150000001875 compounds Chemical class 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
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- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
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- 229960001763 zinc sulfate Drugs 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
本発明はバラ属植物の特定の部位を特定の培養条件のも
とに組織培養してバラ属植物の種苗を増殖する方法に関
する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a method for propagating seeds and seedlings of plants of the genus Rosa by tissue culturing a specific part of the plant under specific culture conditions.
バラ属植物は園芸植物として鑑賞用に愛好されている。 Plants of the genus Rose are loved as ornamental garden plants.
該植物の種菌の生産と増殖は従来挿し木によって行なわ
れてきた。しかし、このような種苗の生産と増殖は多く
の人手と土地を必要とするばかりでなく、近年ではウィ
ルス病の蔓延により種苗の生育速度の低下や花の品質低
下が問題となっている。近年植物組織培養技術を利用し
て種々の植物を培養することが行われているが、バラ属
植物についてはその応用例はあまり報告されていない。Production and propagation of inoculum of the plant have conventionally been carried out by cuttings. However, the production and propagation of such seedlings not only requires a lot of manpower and land, but in recent years, the spread of viral diseases has caused problems such as a slowdown in the growth rate of seedlings and a decline in the quality of flowers. In recent years, various plants have been cultivated using plant tissue culture technology, but there are not many reported examples of its application to plants of the genus Rosa.
〔発明が解決しよ・うとする課題］
本発明者等は、従来行われているバラ属植物の種苗生産
方法では生産効率が悪く、しかも高品質のものが得られ
ないことを認めたので、組織培養技術を応用して従来法
にかわるべく生産効率が高くしかも高品質のバラ属植物
種苗の増殖方法について検制した。[Problems to be Solved by the Invention] The present inventors have recognized that the conventional methods of producing seeds and seedlings of plants of the genus Rosa have poor production efficiency and cannot produce high-quality products. By applying tissue culture technology, we tested a method for propagating rose seedlings with high production efficiency and high quality in place of conventional methods.
その結果、本発明者等は下記方法を用いれば前記目的を
達成できることを見出し本発明を完成するに到った。す
なわち本発明によれば、バラ属植物の分離された側芽な
いし側芽を含む組織片をサイトカイニンを含む、液体培
地中で通気培養することにより側芽を増殖することを特
徴とするバラ属植物の種苗の増殖方法が提供される。As a result, the present inventors discovered that the above object could be achieved by using the method described below, and completed the present invention. That is, according to the present invention, there is provided a seedling of a plant of the genus Rosa, which is characterized in that the lateral buds are multiplied by aeration culturing a separated lateral bud or a tissue piece containing the lateral bud of the plant in a liquid medium containing cytokinin. A propagation method is provided.
本発明のバラ属植物の種苗の増殖方法が適用できる植物
は、バラ属に含まれる植物であって、具体的には、ハイ
ブリッドティーローズ、グランシフローラ、フロリブン
ダなどの大、中輪バラやクライミングハイブリットティ
ーローズ、ランブラ−などのツルバラおよびマリーアン
トワ゛ネント、ハイジー、シンブレラなどのミニバラに
属する植物を例示できる。Plants to which the method of propagating seeds and seedlings of plants belonging to the genus Rosa of the present invention can be applied are plants included in the genus Rosa, and specifically include large and medium-sized roses such as hybrid tea roses, grandiflora, and floribunda, and climbing roses. Examples include climbing roses such as hybrid tea roses and ramblers, and plants belonging to mini roses such as Marie Antoine, Hygiene, and Simbrella.
本発明ではバラ属植物の種苗の増殖を行うに当たっては
、バラ属植物の器官の中でも特定の組織が用いられる。In the present invention, when propagating seeds and seedlings of plants of the genus Rosa, specific tissues among the organs of plants of the genus Rosa are used.
すなわち本発明ではバラ属植物の分離された側芽ないし
側芽を含む組織片が′Ｍｉ織培前培養料に用いられる。That is, in the present invention, isolated lateral buds or tissue pieces containing lateral buds of plants of the genus Rosa are used as a pre-culture material for 'Mi tissue culture.
前記側芽ないし側芽を含む組織片以外の組織片を培養の
材料に用いても苗条を育成することは困難である。本発
明ではこの側芽ないし側芽を含む組織片を特に液体培地
を用いて培養する。これは寒天のような固体培地を用い
て側芽ないし側芽を含む組織片を培養しても、苗条の成
育速度が遅く、又培養の途中で壊死する率も高いからで
ある。前記液体培地としては植物ホルモンのサイＩ・カ
イニンを含む液体培地が用いられる。す′イＩ・カイニ
ンとしてカイネチン８１、セニルアミノプリン９２、フ
ェニルアミノプリン、ヘンシルアデニン（Ｂ八）１１、
ペンチルアミノプリン２−ナフチルメチルアミノプリン
、シクロへキシルアミノプリン、０−クロロヘンシルア
ミノプリン、０−メチルヘンシルアミノプリンなどの合
成サイトカイニン、６−イツペンテニルアミノプリン、
トランスーセアヂン、シスーゼアヂン、２−メチルチオ
−６−イツペンテニルアミノプリン、プリン−６−カー
バモイルスレオニン、Ｏ−ヒドロシキー、フシルアミノ
−９−β−リボフラノシルプリンなどの天然サイトカイ
ニンを例示できる。Even if tissue pieces other than the lateral buds or tissue pieces containing lateral buds are used as culture materials, it is difficult to grow shoots. In the present invention, this lateral bud or a tissue piece containing a lateral bud is particularly cultured using a liquid medium. This is because even if lateral buds or tissue pieces containing lateral buds are cultured using a solid medium such as agar, the growth rate of the shoots is slow and the rate of necrosis during cultivation is high. As the liquid medium, a liquid medium containing the plant hormone Cy-I/Kainin is used. Kinetin 81, senylaminopurine 92, phenylaminopurine, hensyl adenine (B8) 11,
Synthetic cytokinins such as pentylaminopurine 2-naphthylmethylaminopurine, cyclohexylaminopurine, 0-chlorohensylaminopurine, 0-methylhensylaminopurine, 6-itpentenylaminopurine,
Examples include natural cytokinins such as trans-theadin, cis-seadin, 2-methylthio-6-itpentenylaminopurine, purine-6-carbamoylthreonine, O-hydroxyky, and fusylamino-9-β-ribofuranosylpurine.
（来夏以下余白）
■
本発明においては、前記サイ１〜カイニンの中、特にヘ
ンシルアデニンが好適に使用される。サイトカイニンの
使用割合としては通常は１０− ’Ｍ／ ｆｆｉ〜１０
−！′Ｍ＃２、好ましくは１０−７Ｍ＃！〜１０ー’Ｍ
／Ｉ！．の範囲にある。サイトカイニンを含まない培地
を用いて側芽を培養した場合には、後述の実施例と比較
例からも明らかなように、この側芽から新たな側芽が形
成されないので種苗の増殖を行うことができないことか
ら上記のように本発明ではサイトカイニンを含有する培
地が用いられるのである。(Blank below next summer) (2) In the present invention, among the above-mentioned Sai1 to Kainin, hensyl adenine is particularly preferably used. The usage rate of cytokinin is usually 10-'M/ffi~10
-! 'M#2, preferably 10-7M#! ~10-'M
/I! ．． within the range of When lateral buds are cultured using a medium that does not contain cytokinin, as is clear from the Examples and Comparative Examples described later, new lateral buds are not formed from these lateral buds, making it impossible to propagate seedlings. As mentioned above, a medium containing cytokinin is used in the present invention.
本発明におけるバラ属植物の側芽ないし側芽を含む組織
片の組織培養では、好ましくは、液体培地に酸素含有ガ
スを通気して培養が行われる。通気しないで培養した場
合には側芽の形成数が少ないので好ましくない。この場
合の酸素含有気体としては例えば酸素や空気を単独に用
いたり、酸素、空気、チッ素、二酸化炭素などのうちの
２種類以上の気体を混合した気体を用いることができる
。In the tissue culture of a lateral bud or a tissue piece containing a lateral bud of a plant of the genus Rosa in the present invention, the culture is preferably carried out by aerating an oxygen-containing gas into the liquid medium. It is not preferable to culture without aeration because the number of side buds formed is small. As the oxygen-containing gas in this case, for example, oxygen or air can be used alone, or a mixture of two or more types of gases such as oxygen, air, nitrogen, and carbon dioxide can be used.
本発明ではこれらの気体中の酸素含有量は通常５〜１０
０Ｖｏ１％、より好ましくは２０〜１００νｏ１％であ
る。In the present invention, the oxygen content in these gases is usually 5 to 10
It is 0Vo1%, more preferably 20 to 100Vo1%.
前記気体の液体培地中への通気速度としては、培養器の
形状によって多少異なるが、一般に酸素移動容量係数（
ＫＬａ）で表示して通常は０． １〜３０程度、特に好
ましくは１〜３程度になるように調節して組織培養が行
われる。The aeration rate of the gas into the liquid medium varies somewhat depending on the shape of the culture vessel, but is generally determined by the oxygen transfer capacity coefficient (
KLa) and is normally 0. Tissue culture is performed with the concentration adjusted to about 1 to 30, particularly preferably about 1 to 3.
本発明では培養物に加えられる外力をできるだけ小さく
するために、ＫＬａの値を大きくして培養を行う場合に
は酸素含有ガス中の酸素濃度を高めて、該ガスの単位時
間当たりの通気量を大きくしないようにすることが好ま
しい。本発明では酸素含有カスの通気量としては液体培
地の単位容積（り、単位時間当たり通常１００ないし３
００００ ｍｌ／ρ・ｈｒ、好ましくは１０００〜５０
００ｍｌ／ｐ、・ｈｒの範囲にあるようにする。通気量
が３００００以上の場合乙こは培養物が損傷し易くこの
ときには高品質の種苗が得にくいこと、又１００以下の
場合にはｌａの値を前記０．１〜３０程度にすることが
困難であることから前述のような通気量を選ぶことが好
ましい。In the present invention, in order to minimize the external force applied to the culture, when culturing is performed with a large value of KLa, the oxygen concentration in the oxygen-containing gas is increased and the amount of aeration of the gas per unit time is reduced. It is preferable not to make it large. In the present invention, the aeration rate of oxygen-containing waste is usually 10 to 3
0000 ml/ρ・hr, preferably 1000-50
00ml/p, hr. If the aeration rate is 30,000 or more, the culture is likely to be damaged and it is difficult to obtain high quality seedlings, and if the aeration rate is less than 100, it is difficult to maintain the value of la to about 0.1 to 30. Therefore, it is preferable to select the amount of ventilation as described above.
本発明におけるバラ属植物の側芽ないし側芽を含む組織
片の培養は光照射下で行われることが好ましい。この場
合の光強度としては通常１００〜１５００ルツクスであ
り、好ましくは２００〜８００ルツクスの範囲である。In the present invention, culturing of a lateral bud or a tissue piece containing a lateral bud of a plant of the genus Rosa is preferably carried out under light irradiation. The light intensity in this case is usually 100 to 1500 lux, preferably 200 to 800 lux.
本発明では光照射下に培養すると側芽の形成数が増大す
るので好ましい。また本発明では光照射下に培養を行う
場合には、１００〜５００ルツクスの明期を通常１２〜
１６時間、０〜１００ルツクスの暗照が通常８〜１２時
間となるようにして培養すると側芽形成数が増大し、安
定な苗条が得られるので好ましい。In the present invention, culturing under light irradiation is preferred because the number of lateral buds formed increases. In addition, in the present invention, when culturing is carried out under light irradiation, the light period of 100 to 500 lux is usually
It is preferable to culture with 16 hours of dark light of 0 to 100 lux, usually 8 to 12 hours, because the number of lateral buds formed increases and stable shoots are obtained.
本発明では前記方法によって得られる側芽をたくさんつ
けた苗条は、その適宜量を切断して側芽ないし側芽を含
む組織片として再度循環使用して前記培養方法と同様の
培養を繰り返すことによってバラ属植物の側芽を含む苗
条を短期間に効率良く大量に増殖することができる。又
このようにして増殖させた側芽を含む苗条は、液体培地
で適宜大きさに育成させた後、液体培地から取り出して
バーライ１〜、バーミキュライト、ロックウールなどの
各種の支持体にごの苗条を挿入して、従来から知られて
いる発根、馴化雰囲気中で適宜期間育成させた後、通常
の栽培を行な・うと、性質が一定で健全な植物体に成長
させることができる。In the present invention, the shoots with many lateral buds obtained by the above method are cut off in an appropriate amount and reused as lateral buds or tissue pieces containing lateral buds, and the same culture as in the above culture method is repeated to produce plants of the genus Rosa. It is possible to efficiently propagate a large amount of shoots including lateral buds in a short period of time. The shoots containing lateral buds propagated in this manner are grown to an appropriate size in a liquid medium, and then removed from the liquid medium and placed on various supports such as Barley 1, vermiculite, and rock wool. After inserting the seedlings, cultivating them for an appropriate period in a conventionally known rooting and acclimatization atmosphere, and then carrying out normal cultivation, it is possible to grow a healthy plant with constant characteristics.
次に本発明で使用される液体培地について以下具体的に
示す。Next, the liquid medium used in the present invention will be specifically described below.
本発明で使用される培地は、無機成分および炭素源を必
須成分とし、これに植物ホルモン類、ビタミン類を添加
し、更に必要に応してアミノ酸類を添加した培地である
。該培地の無機成分とじては、窒素、リン、カリウム、
ナトリウム、カルシウム、マグネシウム、イオウ、鉄、
マンガン、亜鉛、ホウ素、モリブデン、塩素、ヨウ素、
コバルト等の元素を含む無機塩を挙げることができ、具
体的には硝酸カリウム、硝酸ナトリウム、硝酸アンモニ
ウム、塩化アンモニウム、塩化カリウム、塩化カルシウ
ム、リン酸１水素カリウム、リン酸２水素ナトリウム、
硫酸マグネシウム、塩化マグネシウム、硫酸ナトリウム
、硫酸第１鉄、硫酸第２鉄、硫酸マンガン、硫酸銅、モ
リブデン酸ナトリウム、三酸化モリブデン、ヨウ化カリ
ウム、硫酸亜鉛、ホウ酸、塩化コバルト等の化合物を例
示できる。The medium used in the present invention is a medium containing inorganic components and a carbon source as essential components, to which are added plant hormones and vitamins, and further added with amino acids as necessary. Inorganic components of the medium include nitrogen, phosphorus, potassium,
Sodium, calcium, magnesium, sulfur, iron,
Manganese, zinc, boron, molybdenum, chlorine, iodine,
Examples include inorganic salts containing elements such as cobalt, specifically potassium nitrate, sodium nitrate, ammonium nitrate, ammonium chloride, potassium chloride, calcium chloride, potassium monohydrogen phosphate, sodium dihydrogen phosphate,
Examples of compounds include magnesium sulfate, magnesium chloride, sodium sulfate, ferrous sulfate, ferric sulfate, manganese sulfate, copper sulfate, sodium molybdate, molybdenum trioxide, potassium iodide, zinc sulfate, boric acid, and cobalt chloride. can.
該培地の炭素源としては、ショ糖等の炭水化物とその誘
導体、脂肪酸等の有機酸およびエタノール等の１級アル
コールなどを例示できる。Examples of carbon sources for the medium include carbohydrates such as sucrose and derivatives thereof, organic acids such as fatty acids, and primary alcohols such as ethanol.
該培地の植物ホルモン類としては、本発明では前述の如
くサイトカイニンが用いられる。又必要に応じてジベレ
リンを含有させても良い。In the present invention, cytokinin is used as the plant hormone in the medium, as described above. Furthermore, gibberellin may be included if necessary.
該培地のビタミン類としては、ビオチン、チアミン（ビ
タミンＢ、）、ピリドキシン（ビタミン８６）、ピリ［
′キ→）−−ル、ピリドキサミン、パントテン酸カルシ
ウム、アスコルビン酸（ビタミンＣ）、イノシト−ル、
ニコチン酸、ニコチン酸アミドおよびリボフラビン（ビ
タミンＢ２）などを例示できる。The vitamins in the medium include biotin, thiamine (vitamin B), pyridoxine (vitamin 86), and pyridoxine (vitamin 86).
'ki→)--ol, pyridoxamine, calcium pantothenate, ascorbic acid (vitamin C), inositol,
Examples include nicotinic acid, nicotinamide, and riboflavin (vitamin B2).
該培地のアミノ酸類としては、例えばグリシン、アラニ
ン、グルタミン酸、システィン、フェニルアラニンおよ
びリジンなどを例示できる。Examples of amino acids in the medium include glycine, alanine, glutamic acid, cysteine, phenylalanine, and lysine.
本発明の前記培地は、通常は、前記無機成分を約０．１
μ門ないし約１００ｍＭ 、前記炭素源を約１ｇ／ｌな
いし約１００ｇ／４２、前記植物ホルモン類を約０．０
１ｍｇ／４２ないし約１０ｍｇ／ｆｆｉ、前記ビタミン
類を約０．１ ｍｇ／ ｌないし約１５０ｍｇ／、ｆ！
および前記アミノ酸類をＯないし約１００ｍｇ／ρ含ま
せて使用されることが望ましい。The medium of the present invention usually contains about 0.1 of the inorganic components.
μ to about 100mM, about 1g/l to about 100g/42 of the carbon source, about 0.0g/l to about 0.0g of the plant hormones.
1 mg/42 to about 10 mg/ffi, about 0.1 mg/l to about 150 mg/f of the above vitamins, f!
It is desirable that the amino acids are used in an amount of 0 to about 100 mg/ρ.
本発明に用いられる前記培地として具体的には、従来か
ら知られている植物の組織培養に用いられている培地、
例えば、ムラシゲ・スクーグ（’ ６２）（Ｍｕｒａｓ
ｈｉｇｅ ＆ Ｓｋｏｏｇ）の培地、リンスマイヤー・
スクーグ（ＲＭ−１９６５） ［Ｌｉｎｓｍａｉｅｒ
＆ Ｓｋｏｏｇ］の培地、ホワイト （”６３） （Ｗ
ｈ ｉ ｔｅ 〕の培地、ガンホルグ［Ｇａｍｂｏｒｇ
］のＢ−５培地、三片のト９培地、−−ｙチ・エッチ
の培地（Ｎ１ｔｃｌ＋ ＆ Ｎ１ｔｃｈ ］等乙こ前
記した炭素源および植物ホルモンを添加し、更に必要に
応して前記したビタミン類、アミノ酸類を添加して調製
される培地を例示できるが、本発明ではこの中でも特乙
こニッチ・ニッチ、リンスマ・イヤー・スクーグ又はム
ラシゲ・スクーグの培地を用いて調製される培地が好ま
しい。なお、干記した従来公知の培地の組成に関しては
、例えば、作因、中島、古谷著の「新植物Ｓ、■織培養
、 ｐ３８６〜ｐ３９１、朝倉書店、１９７９年に記載
されている。Specifically, the medium used in the present invention includes a conventionally known culture medium used for plant tissue culture;
For example, Murashige Skoog ('62)
hige & Skoog), Linsmeyer
Skoog (RM-1965) [Linsmaier
& Skoog] medium, white (”63) (W
h ite] medium, Gamborg [Gamborg]
] B-5 medium, Mikata's To9 medium, --ychi-etch medium (N1tcl+ & N1tch), etc. Add the above-mentioned carbon sources and plant hormones, and further add the above-mentioned vitamins as necessary. Among these, preferred in the present invention are media prepared using Tokuotoko Niche Niche, Linsma Ear Skoog, or Murashige Skoog medium. The composition of the conventionally known culture medium mentioned above is described, for example, in "New Plant S," by Nakajima and Furuya, p.386-p.391, Asakura Shoten, 1979.
以下、本発明の方法を実施例によって具体的に説明する
。Hereinafter, the method of the present invention will be specifically explained using examples.
実施例１〜４
ミニバラのマリーアントワネントの茎ヲ滅菌シて側芽を
採取し、これをショ１ｌｊｉ ３％を含む１、ラシゲ
スクーグの培地に表１に示す温度のヘンシルアデニン（
ＢＡ）を添加したｐｌ−１５’、 ７の無菌の液体培地
を調製し７この培地をガラス製の容器に入れ、これに先
のバラの側芽を加えて、２５°Ｃで１６時間明朋（５０
０１ＬＩＸ） ８１１’４間暗）Ｕｌの光条件下で酸素
を通気して４週間培養した。培養した１個の側芽から再
生した植物体の側芽数を測定したところ表１に示す結果
を得た。Examples 1 to 4 The stems of miniature roses Marie Antoinent were sterilized and the side buds were collected, and this was then treated with 1.
Hensyl adenine (
Prepare a sterile liquid medium of pl-15', 7 supplemented with BA), place this medium in a glass container, add the lateral buds of the rose, and incubate at 25°C for 16 hours. 50
The cells were cultured for 4 weeks under the light conditions of 01LIX)811'4dark)U1 and aeration of oxygen. The number of lateral buds of plants regenerated from one cultured lateral bud was measured, and the results shown in Table 1 were obtained.
実施例５
賄里−ド（１ｆ１養１〜だ以外Ｄａ実施例１〜４と同様
乙こして行った。Example 5 The same procedure as in Examples 1 to 4 was carried out except that the temperature was 1f1.
比較例１〜２
培地にヘンシルアデニンを冷力ｌしないこと、暗黒下で
培養すること、あるいＬ；１酸素を通気しなし・こと以
外は該実施例と同様にして培養した結果を表１に示し２
だ。Comparative Examples 1 to 2 The results of culturing hensyl adenine in the culture medium in the same manner as in the examples except that the culture medium was not subjected to cold treatment, cultured in the dark, or without aeration of oxygen are shown below. Shown in 1 and 2
is.
実施例（ｊ〜９
４、＋ ｉ′：ｌとし７て中輪バラの−１−ヤンディア
の側芽を用いること以外は該実施例１〜４よ同様にして
培養した結果を表Ｈこ示した。Table H shows the results of culturing in the same manner as in Examples 1 to 4, except that lateral buds of -1-Yandia, a medium-sized rose, were used as Example (j~9 4, + i':l). .
比較１列３
実施例５−８’こおいて−・〕／ジルアデニンを／ｋｉ
ない培地を用い、酸素を通気せずに暗黒下で培養するこ
と以外は該実施例と同様にして培養した結果を表１に示
した。Comparison 1 row 3 Example 5-8'Koto-・]/dyladenine/ki
Table 1 shows the results of culturing in the same manner as in Example 1, except that the cells were cultured in a dark medium without oxygen aeration.
（来夏以Ｔ余白）
表 １
実施例］Ｏ
培地０．３μ門のヘンシルアデニンと１μ門のシヘレリ
ン酸を含む以夕＋ば該実施例２と同様にしてミニバラの
マリーアントワ不ノトの側芽を培檜した結果を表２に示
した。(T margin from next summer) Table 1 Example] O Medium containing 0.3 μm of hensyl adenine and 1 μm of schiherelic acid. Table 2 shows the results of culturing in hinoki.
実施例１１
培地にジヘレリン酸を添加しない以り口Ａ該実施例１０
と同様にして培養した結果を表２に示し７た。Example 11 Example 10 without adding diherelic acid to the medium
The results of culturing in the same manner as above are shown in Table 2.
表 ２
〔発明の効果」
本発明の方法によれば、バラ属植物の側芽を短期間で効
率良く大量に増殖することができ、したがってこの方法
Ｑこよりバラ属植物の種苗を大量に生産効率を高くして
増殖することができる。Table 2 [Effects of the Invention] According to the method of the present invention, the lateral buds of plants of the genus Rosa can be efficiently multiplied in large quantities in a short period of time. It can grow taller and grow.
Claims (1)
片をサイトカイニンを含む液体培地中で通気培養するこ
とにより側芽を増殖することを特徴とするバラ属植物の
種苗の増殖方法。 ２、サイトカイニンがベンジルアデニンであることを特
徴とする請求項１記載のバラ属植物の種苗の増殖方法。 ３、ベルジルアデニンの濃度が１０＾−＾８Ｍ／ｌ〜１
０＾−＾６Ｍ／ｌであることを特徴とする請求項２記載
のバラ属植物の種苗の増殖方法。[Scope of Claims] 1. Seedlings of plants of the genus Rosa, characterized in that the lateral buds are multiplied by aeration culturing isolated lateral buds or tissue pieces containing lateral buds of plants of the genus Rosa in a liquid medium containing cytokinin. Propagation method. 2. The method for propagating seeds and seedlings of plants of the genus Rosa according to claim 1, wherein the cytokinin is benzyladenine. 3. The concentration of verzyladenine is 10^-^8M/l ~ 1
The method for propagating seeds and seedlings of plants of the genus Rosa according to claim 2, characterized in that the concentration is 0^-^6M/l.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63098358A JPH01269430A (en) | 1988-04-22 | 1988-04-22 | Method and propagating shoot of plant belonging to genus rosa |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63098358A JPH01269430A (en) | 1988-04-22 | 1988-04-22 | Method and propagating shoot of plant belonging to genus rosa |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH01269430A true JPH01269430A (en) | 1989-10-26 |
Family
ID=14217663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63098358A Pending JPH01269430A (en) | 1988-04-22 | 1988-04-22 | Method and propagating shoot of plant belonging to genus rosa |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01269430A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104350934A (en) * | 2014-11-27 | 2015-02-18 | 遵义市石锐花卉种植园 | Method for culturing seedlings of rose |
| CN104429521A (en) * | 2014-11-27 | 2015-03-25 | 遵义市石锐花卉种植园 | Rose cultivation method |
| CN104429494A (en) * | 2014-11-14 | 2015-03-25 | 何勇 | Dipsacus asperoids rapid seedling method |
-
1988
- 1988-04-22 JP JP63098358A patent/JPH01269430A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104429494A (en) * | 2014-11-14 | 2015-03-25 | 何勇 | Dipsacus asperoids rapid seedling method |
| CN104429494B (en) * | 2014-11-14 | 2016-05-04 | 何勇 | A kind of teasel root fast seedling-cultivating method |
| CN104350934A (en) * | 2014-11-27 | 2015-02-18 | 遵义市石锐花卉种植园 | Method for culturing seedlings of rose |
| CN104429521A (en) * | 2014-11-27 | 2015-03-25 | 遵义市石锐花卉种植园 | Rose cultivation method |
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