JPH01195357A - Electrophoretic device - Google Patents
Electrophoretic deviceInfo
- Publication number
- JPH01195357A JPH01195357A JP63021042A JP2104288A JPH01195357A JP H01195357 A JPH01195357 A JP H01195357A JP 63021042 A JP63021042 A JP 63021042A JP 2104288 A JP2104288 A JP 2104288A JP H01195357 A JPH01195357 A JP H01195357A
- Authority
- JP
- Japan
- Prior art keywords
- temp
- voltage
- temperature
- upper limit
- migration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001962 electrophoresis Methods 0.000 claims description 16
- 238000001514 detection method Methods 0.000 claims description 4
- 238000001816 cooling Methods 0.000 abstract description 9
- 238000013508 migration Methods 0.000 abstract description 7
- 230000005012 migration Effects 0.000 abstract description 7
- 239000012530 fluid Substances 0.000 description 3
- 238000001155 isoelectric focusing Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000000919 ceramic Substances 0.000 description 2
- 239000000498 cooling water Substances 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- 239000008151 electrolyte solution Substances 0.000 description 2
- LDHMAVIPBRSVRG-UHFFFAOYSA-O 1-methylnicotinamide Chemical compound C[N+]1=CC=CC(C(N)=O)=C1 LDHMAVIPBRSVRG-UHFFFAOYSA-O 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000009501 film coating Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
Landscapes
- Investigating Or Analyzing Materials Using Thermal Means (AREA)
Abstract
Description
【発明の詳細な説明】
(イ)産業上の利用分野
本発明は9等電点電気泳動装置の、特に温度調整に関す
る。DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application The present invention relates to a nine isoelectric focusing device, particularly temperature control.
(ロ)従来技術
等電点電気泳動装置は、 ゲル媒体に試
料を置き両端に電圧を印加して、試料中の各物質を等電
点に対応するpHの位置に泳動させるものである。(b) A prior art isoelectric focusing device is one in which a sample is placed in a gel medium and a voltage is applied to both ends of the gel medium to cause each substance in the sample to migrate to a pH position corresponding to its isoelectric point.
ゲI′v媒体の温度は、試料の泳動度に影響し、グ〃の
温度が変化すれば試料の泳動度も変化する。The temperature of the medium influences the electrophoretic mobility of the sample, and as the temperature of the medium changes, the electrophoretic mobility of the sample also changes.
また1等電点電気泳動の場合、pHは温度の影響を受け
るので再現性を考慮すると泳動中恒温状態が保たれるこ
とが望ましい。In addition, in the case of isoelectric focusing, pH is affected by temperature, so in consideration of reproducibility, it is desirable to maintain a constant temperature state during electrophoresis.
そこで従来は、ゲ/L’媒体に温調装置を備え、該温調
装置に流す循環液の温度を制御してゲル媒体を恒温状態
に保っていた。ここで使用する循環液としては例えば1
0″Cの冷却水を使用していた。Conventionally, the gel/L' medium was equipped with a temperature control device, and the temperature of the circulating fluid flowing through the temperature control device was controlled to maintain the gel medium at a constant temperature. For example, the circulating fluid used here is 1
0″C cooling water was used.
また、これ以外の方法として温調用循環液の制御に加え
泳動の際に供給する電力によシ温度制御する方法がある
。これは、電力を所定のワット数に設定し、設定ワット
数になる様に電圧を制御するものである。一般にゲ/L
/媒体は時間の経過につれて抵抗が大きくなるので、電
圧を階段的に上げて電力を一定に保っている。Further, as a method other than this, there is a method of controlling the temperature by using electric power supplied during electrophoresis in addition to controlling the circulating liquid for temperature adjustment. This sets the power to a predetermined wattage and controls the voltage to reach the set wattage. Generally gay/L
/Since the resistance of the medium increases over time, the voltage is increased stepwise to keep the power constant.
(ハ)発′明が解決しようとする課題
温調用循環液の温度を制御する・方式では、電気泳動用
分離媒体であるポリアクリルアミドは熱伝導が悪いので
正確に泳動中のゲルの温度を制御しているとは言えなか
った。(c) Problems to be Solved by the Invention In the method of controlling the temperature of the circulating fluid for temperature regulation, polyacrylamide, which is a separation medium for electrophoresis, has poor heat conduction, so the temperature of the gel during electrophoresis can be accurately controlled. I couldn't say that I was doing it.
また、電力を使った電圧制御では与えるエネルギーは制
御できるが実際のゲル自体の温度は制御しきれていなか
った。これは、ゲルが置かれている熱的環境については
考慮していなかったからである。従って1例えばゲルの
温度を10″Cに保つ様に制御しても+5〜10℃の誤
差があった。In addition, although voltage control using electricity can control the energy applied, the actual temperature of the gel itself cannot be fully controlled. This is because the thermal environment in which the gel is placed was not considered. Therefore, for example, even if the temperature of the gel was controlled to be maintained at 10"C, there was an error of +5 to 10C.
本発明は、ゲルの実際の温度を検知してこの値に基づい
て電力制御を行うことを目的とする。The object of the present invention is to detect the actual temperature of the gel and perform power control based on this value.
に)課題を解決するための手段
本発明は、泳動用ゲル板に温調装置を付設させた電気泳
動装置において、ゲル媒体自体の温度を検知する温度検
知手段と、該温度検知手段の信号に基づき電圧制御を行
う制御手段を備えたことを特徴とする。B) Means for Solving the Problems The present invention provides an electrophoresis apparatus in which a temperature control device is attached to a gel plate for electrophoresis. The present invention is characterized in that it includes a control means that performs voltage control based on the voltage control.
ここで温度検知手段としては、熱電対が使用され、熱電
対は泳動中のゲ/l’を直接計測するのでフッ素樹脂や
セラミックの薄膜コーチインク(100〜200μm)
で厳密に絶縁する。Here, a thermocouple is used as the temperature detection means, and since the thermocouple directly measures Ge/l' during migration, it is necessary to
be strictly insulated.
(ホ)作用
本発明は、温度検知手段でゲfi/媒体自体の温度、を
検知し、温調装置で制御している温度範囲に入っている
か否か監視する。(E) Function In the present invention, the temperature of the medium itself is detected by the temperature detection means, and it is monitored whether the temperature is within the temperature range controlled by the temperature control device.
制御温度範囲を逸脱していれば電圧制御によりその逸脱
分をキャンセルする。If it deviates from the control temperature range, the deviation is canceled by voltage control.
(へ)実施例 本発明の実施例を第1図に示す。(f) Example An embodiment of the invention is shown in FIG.
1が泳動用ゲルで9例えば、 SiCやアルミナなどの
セラミックスで形成されたケースに収容されている。1 is a gel for electrophoresis, and 9 is housed in a case made of ceramics such as SiC or alumina.
泳動用ゲA/1には冷却ブロック2が設置されておシ、
冷却ブロックには冷却水が循環される。冷却ブロック2
の温度はサーモモジ、−/L/3で調整される。A cooling block 2 is installed in the electrophoresis gear A/1.
Cooling water is circulated through the cooling block. cooling block 2
The temperature is adjusted with a thermomoji, -/L/3.
4がゲル自体の温度を検知する熱電対で、熱電対4はフ
ッ素樹脂あるいはセラミックの薄膜コーチイン゛グで絶
縁されている。熱電対4の信号はCPU8で監視される
っ
またCPU8は、冷却ブロック2の温度も監視しておシ
、熱電対4からの泳動温度と相撲って高圧電源7を制御
する。Thermocouple 4 detects the temperature of the gel itself, and thermocouple 4 is insulated with a thin film coating of fluororesin or ceramic. The signal from the thermocouple 4 is monitored by the CPU 8, and the CPU 8 also monitors the temperature of the cooling block 2, and controls the high voltage power supply 7 based on the migration temperature from the thermocouple 4.
なお、5..6は電解液用の溝で、5には電解液として
0.05 Mアスパラギン酸が、6には0.1 MNa
OHが入れられている。In addition, 5. .. 6 is a groove for the electrolyte, 5 contains 0.05 M aspartic acid as the electrolyte, and 6 contains 0.1 MNa.
OH is included.
電解液5には陽樹、6には陰極が接続される。A positive electrode is connected to the electrolytic solution 5, and a cathode is connected to the electrolytic solution 6.
以、上の構成において温度制御は次の様に行う。In the above configuration, temperature control is performed as follows.
まず、泳動温度の許容範囲を設定し、 CPU8で熱電
対4を介して泳動温度をモニタし高圧電源7と冷却ブロ
ック2の双方を監視する。First, an allowable range of electrophoresis temperature is set, and the CPU 8 monitors the electrophoresis temperature via the thermocouple 4 to monitor both the high voltage power supply 7 and the cooling block 2.
泳動温度が許容範囲なら、その上限に達するまで電圧を
上げる。一般に分離能は電位勾配に比例し電圧が高い根
分#能が向上するからである。If the running temperature is within an acceptable range, increase the voltage until the upper limit is reached. This is because, in general, the separation power is proportional to the potential gradient, and the higher the voltage, the better the separation power becomes.
許容範囲の上限に達したら熱が拡散し冷却温度(許容範
囲の下限)と同一温度になるまで一定電までくり返した
後定電圧に保つ。When the upper limit of the allowable range is reached, the heat is diffused and the voltage is kept at a constant voltage until the temperature reaches the same as the cooling temperature (lower limit of the allowable range).
但し、定電圧状態で上限温度を超える場合は電圧を一度
下げ、状態が安定してから再度同様にして電圧を印加上
限まで上げる。However, if the upper limit temperature is exceeded in a constant voltage state, the voltage is lowered once, and after the condition becomes stable, the voltage is raised again to the upper limit.
なお、これまでの説明では熱電対4は一箇所しか設けら
れていなかったが、ゲ/I/1に等間隔に複数本設置し
これらの平均値を取る様にしても良い。In addition, in the explanation so far, the thermocouple 4 is provided at only one location, but a plurality of thermocouples may be provided at equal intervals on Ge/I/1 and the average value of these thermocouples may be taken.
また、ゲ/L/1の抵抗の一番大きい箇所、つまシ温度
の一番上昇する箇所を検出しそこに設置しても良い。Alternatively, the location where the resistance of Ge/L/1 is the highest or the location where the tab temperature increases the most may be detected and installed there.
(ト)効果
本発明は、ゲルの温度を直接モニタし電圧を制御するの
で常に恒温状態で泳動ができる。(G) Effect The present invention directly monitors the temperature of the gel and controls the voltage, so that electrophoresis can always be performed in a constant temperature state.
また、熱的環境が変化しても常に再現性あるデータが得
られる。In addition, reproducible data can always be obtained even if the thermal environment changes.
第1図は1本発明に係る電気泳動装置の概略図を示す。 1−泳動用ゲ/I/2−・冷却ブロック4−・熱電対 FIG. 1 shows a schematic diagram of an electrophoresis apparatus according to the present invention. 1-Electrophoresis gauge/I/2-・Cooling block 4-・Thermocouple
Claims (1)
において、 ゲル媒体自体の温度を検知する温度検知手段と、該温度
検知手段の信号に基づき電圧制御を行う制御手段を備え
たことを特徴とする電気泳動装置。[Scope of Claims] 1. In an electrophoresis apparatus in which a temperature control device is attached to a gel plate for electrophoresis, there is provided a temperature detection means for detecting the temperature of the gel medium itself, and voltage control is performed based on a signal from the temperature detection means. An electrophoresis device characterized by comprising a control means.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP63021042A JPH01195357A (en) | 1988-01-29 | 1988-01-29 | Electrophoretic device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP63021042A JPH01195357A (en) | 1988-01-29 | 1988-01-29 | Electrophoretic device |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH01195357A true JPH01195357A (en) | 1989-08-07 |
Family
ID=12043883
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP63021042A Pending JPH01195357A (en) | 1988-01-29 | 1988-01-29 | Electrophoretic device |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH01195357A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005086586A3 (en) * | 2004-03-15 | 2006-07-06 | D N R Imaging Systems Ltd | Method and system for monitoring material separation process such as electrophoresis process in a sample |
WO2013094735A1 (en) | 2011-12-22 | 2013-06-27 | シャープ株式会社 | Control method, control device, control system, and control program |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS49106893A (en) * | 1973-01-24 | 1974-10-09 |
-
1988
- 1988-01-29 JP JP63021042A patent/JPH01195357A/en active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS49106893A (en) * | 1973-01-24 | 1974-10-09 |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005086586A3 (en) * | 2004-03-15 | 2006-07-06 | D N R Imaging Systems Ltd | Method and system for monitoring material separation process such as electrophoresis process in a sample |
US7967968B2 (en) | 2004-03-15 | 2011-06-28 | D.N.R.-Imaging Systems Ltd. | Method and system for monitoring material separation process such as electrophoresis process in a sample |
WO2013094735A1 (en) | 2011-12-22 | 2013-06-27 | シャープ株式会社 | Control method, control device, control system, and control program |
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