JP7425468B2 - Animal surgery vinyl isolator - Google Patents
Animal surgery vinyl isolator Download PDFInfo
- Publication number
- JP7425468B2 JP7425468B2 JP2020000742A JP2020000742A JP7425468B2 JP 7425468 B2 JP7425468 B2 JP 7425468B2 JP 2020000742 A JP2020000742 A JP 2020000742A JP 2020000742 A JP2020000742 A JP 2020000742A JP 7425468 B2 JP7425468 B2 JP 7425468B2
- Authority
- JP
- Japan
- Prior art keywords
- animal
- vinyl
- isolator
- vinyl isolator
- window
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241001465754 Metazoa Species 0.000 title claims description 140
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 title claims description 119
- 229920002554 vinyl polymer Polymers 0.000 title claims description 119
- 238000001356 surgical procedure Methods 0.000 title claims description 50
- 210000001015 abdomen Anatomy 0.000 claims description 24
- 239000002313 adhesive film Substances 0.000 claims description 23
- 210000003754 fetus Anatomy 0.000 claims description 23
- 239000000463 material Substances 0.000 claims description 20
- 238000004519 manufacturing process Methods 0.000 claims description 17
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 claims description 10
- 230000003187 abdominal effect Effects 0.000 claims description 5
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 description 15
- 210000004291 uterus Anatomy 0.000 description 14
- 241001515942 marmosets Species 0.000 description 13
- 238000009802 hysterectomy Methods 0.000 description 10
- 241000282898 Sus scrofa Species 0.000 description 7
- 238000010171 animal model Methods 0.000 description 7
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- KFSLWBXXFJQRDL-UHFFFAOYSA-N Peracetic acid Chemical compound CC(=O)OO KFSLWBXXFJQRDL-UHFFFAOYSA-N 0.000 description 4
- 241000282887 Suidae Species 0.000 description 4
- 239000000853 adhesive Substances 0.000 description 4
- 230000001070 adhesive effect Effects 0.000 description 4
- 239000000645 desinfectant Substances 0.000 description 3
- 210000000936 intestine Anatomy 0.000 description 3
- 210000003101 oviduct Anatomy 0.000 description 3
- 241000700198 Cavia Species 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 239000007779 soft material Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 206010015548 Euthanasia Diseases 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000282575 Gorilla Species 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 241000282567 Macaca fascicularis Species 0.000 description 1
- 241000282560 Macaca mulatta Species 0.000 description 1
- 208000034702 Multiple pregnancies Diseases 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241000282339 Mustela Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241000282579 Pan Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 210000003489 abdominal muscle Anatomy 0.000 description 1
- 210000001691 amnion Anatomy 0.000 description 1
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 244000309715 mini pig Species 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 238000011580 nude mouse model Methods 0.000 description 1
- 238000009400 out breeding Methods 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000010626 work up procedure Methods 0.000 description 1
- 210000002417 xiphoid bone Anatomy 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Materials For Medical Uses (AREA)
Description
本発明は、動物手術用ビニールアイソレータに関する。 The present invention relates to vinyl isolators for veterinary surgery.
実験動物が病源性微生物等特定の微生物に汚染されると繁殖成績や実験結果に大きな影響が出る。汚染された動物から病源微生物を除く方法として、従来、帝王切開法と子宮切断法が知られている。 If laboratory animals become contaminated with specific microorganisms such as pathogenic microorganisms, their reproductive performance and experimental results will be greatly affected. Conventional methods for removing pathogenic microorganisms from contaminated animals include caesarean section and uterine cutting.
小動物の人工出産は、一般的に子宮切除術により行われてきた。一般的な子宮切除術を挙げると、
(i)母体を頸椎脱臼等により安楽死する工程、
(ii)動物の頸椎部分を鉗子で止め、ヨードチンキ等の消毒液の槽に浸す工程、
(iii)消毒後、ヨードチンキ槽から動物を引き上げ、動物表面の消毒液を拭き取る工程、
(iv)動物を解剖台の上にのせて仰向けにし、手足を固定する工程、
(v)ピンセットで腹部中央の腹筋をつまみ、ハサミで正中線に沿って表皮と真皮を同時に切り剣状突起まで切開する工程、
(vi)子宮を取り出す工程、
(vii)鉗子で左右の卵管と膣側を止める工程、
(viii)輪卵管と膣側を止めた鉗子部分の外側をハサミで切断する工程、
(ix)子宮を鉗子で止めた状態で、子宮を摘出する工程、
(x)摘出子宮を消毒液に浸して消毒する工程、を伴う。その後、一般的には、摘出子宮をクリンベンチ内又はビニールアイソレータ内に写し、
(xi)子宮の卵管と膣側の鉗子をはずし、子宮及び羊膜を同時に切開する工程、
(xii)子宮を過酢酸等で洗浄し、薬液槽を通した後、胎仔を摘出する工程、
(xiii)胎仔（新生仔）の体と口腔、鼻孔の羊水を拭き蘇生させる工程、が行われる。
Artificial birth of small animals has generally been performed by hysterectomy. Common hysterectomies include:
(i) The step of euthanizing the mother by cervical dislocation, etc.
(ii) The process of fixing the cervical vertebrae of the animal with forceps and immersing it in a tank of disinfectant such as iodine tincture;
(iii) After disinfection, the step of removing the animal from the iodine tincture tank and wiping off the disinfectant on the surface of the animal;
(iv) placing the animal on its back on a dissection table and fixing the limbs;
(v) Pinch the abdominal muscle in the center of the abdomen with forceps and cut the epidermis and dermis simultaneously along the midline with scissors to make an incision up to the xiphoid process;
(vi) removing the uterus;
(vii) The process of securing the left and right fallopian tubes and the vaginal side with forceps,
(viii) the step of cutting the outside of the forceps part that stopped the ring oviduct and the vagina side with scissors;
(ix) the process of removing the uterus while holding it with forceps;
(x) It involves a step of sterilizing the removed uterus by soaking it in a disinfectant solution. After that, the removed uterus is generally placed in a clean bench or a vinyl isolator, and
(xi) the step of removing the forceps from the oviduct and vaginal side of the uterus and incising the uterus and amniotic membrane at the same time;
(xii) The process of cleaning the uterus with peracetic acid etc. and removing the fetus after passing it through a chemical bath;
(xiii) A process of wiping amniotic fluid from the body, oral cavity, and nostrils of the fetus (newborn) is performed.
このように子宮切除術では、子宮摘出までの工程数が多く、全ての器具をビニールアイソレータ内に配置し、微細な作業を行うのが困難であった。また、子宮内部は基本的には無菌環境であることが知られている。そこで、子宮摘出までの作業をクリンベンチ内で行い、摘出子宮をビニールアイソレータ内又はクリンベンチ内に移し、蘇生を行う方法が採られてきた。 As described above, hysterectomy involves a large number of steps leading up to hysterectomy, and all instruments are placed inside the vinyl isolator, making it difficult to perform delicate work. Furthermore, it is known that the inside of the uterus is basically a sterile environment. Therefore, a method has been adopted in which the work up to hysterectomy is performed in a clean bench, the removed uterus is transferred to a vinyl isolator or a clean bench, and resuscitation is performed.
しかしながら子宮切除術では、母胎は安楽死させるか、又は麻酔する場合も、再び出産することができないため、実験動物倫理上、必ずしも望ましくない。例えば動物実験では、「Replacement（代替）」、「Reduction（削減）」、「Refinement（改善）」の3Rという理念が提唱されている。Reduction（削減）とは、使用する動物の数を削減することや、科学的に必要な最少の動物数を使用する、という理念である。また、Refinement（改善）は、苦痛を軽減すること、安楽死といった措置をとること、飼育環境を改善することを含む理念である。 However, in hysterectomy, even if the mother is euthanized or anesthetized, she cannot give birth again, which is not necessarily desirable in terms of experimental animal ethics. For example, in animal experiments, the 3R philosophy of ``Replacement,'' ``Reduction,'' and ``Refinement'' is advocated. Reduction is the idea of reducing the number of animals used or using the minimum number of animals scientifically necessary. Refinement is a philosophy that includes alleviating suffering, taking measures such as euthanasia, and improving the breeding environment.
一方で、帝王切開であれば母胎を安楽死させる必要もなく、再び出産することが可能である。これは上記の3Rの理念に合致するものである。しかしながら帝王切開術では、母胎が無菌動物でない場合、無菌ではない母胎をビニールアイソレータ内にそのまま持ち込んで作業を行っても汚染が生じてしまう可能性が高い。また無菌ではない母胎を完全に殺菌してビニールアイソレータ内に入れることは容易ではない。さらに、殺菌が成功した場合にも、帝王切開術の全行程をビニールアイソレータ内で行うには、装置が大がかりとなり、作業負担が大きくコスト面でも望ましくない。 On the other hand, with a caesarean section, there is no need to euthanize the mother's fetus, and it is possible to give birth again. This is in line with the 3R philosophy mentioned above. However, in Caesarean sections, if the mother's fetus is not a sterile animal, there is a high possibility that contamination will occur even if the mother's fetus, which is not sterile, is brought into the vinyl isolator and the operation is performed. Furthermore, it is not easy to completely sterilize a mother's fetus, which is not sterile, and place it inside a vinyl isolator. Furthermore, even if sterilization is successful, performing the entire process of caesarean section inside a vinyl isolator requires a large-scale device, which is undesirable from a cost perspective due to the heavy workload.
動物、例えば実験動物又は家畜動物を無菌的に出産する簡便な方法及び装置が求められていた。 There is a need for a simple method and apparatus for aseptically giving birth to animals, such as laboratory or domestic animals.
本発明は、上記の問題を少なくとも部分的に解決することを課題とする。すなわち、本発明は、簡便な動物手術方法及びそのための装置を提供することを課題とする。 The present invention aims to at least partially solve the above problem. That is, an object of the present invention is to provide a simple animal surgery method and an apparatus therefor.
本発明者らは上記の問題について鋭意検討した。子宮切除術ではなく帝王切開法を採用するに当たり、一案として実験動物をビニールアイソレータごと切る方法が考えられる。しかしながら、この方法では、ビニールアイソレータが出産毎に使い捨てとなり、高コストとなる上に資源の浪費となり得る。そこで本発明者らはさらに検討した結果、一例として、手術野用窓を有するビニールアイソレータを用いることにより簡便に動物に帝王切開法を行うことができることを見出し、これを一実施形態として包含する本発明を完成させた。 The present inventors have intensively studied the above problem. When adopting a caesarean section method rather than a hysterectomy, one idea is to cut the experimental animal along with the vinyl isolator. However, with this method, the vinyl isolator is disposable after each birth, which can be costly and a waste of resources. As a result of further investigation, the present inventors found that it is possible to easily perform a caesarean section on an animal by using a vinyl isolator having a window for the surgical field, and the present invention includes this as an embodiment. completed the invention.
さらに、本発明者らは、手術野用窓を有するビニールアイソレータを用いて動物に帝王切開法を行った際に、ビニールアイソレータが波打って光が乱反射し、細かい作業がしにくい、という新規の課題に遭遇した。そこで、本発明者らはさらに検討した結果、一例として、ビニールアイソレータに手術野用窓とは別の観察窓を設けることにより、光の乱反射といった支障なく動物を観察でき手術を素早くできることを見出し、これを一実施形態として包含する本発明を完成させた。 Furthermore, the present inventors discovered a new problem in which when performing a caesarean section on an animal using a vinyl isolator with a surgical field window, the vinyl isolator is wavy and reflects light diffusely, making it difficult to perform detailed work. I encountered a challenge. As a result of further investigation, the inventors of the present invention discovered that, for example, by providing an observation window separate from the window for the surgical field on the vinyl isolator, animals can be observed without problems such as diffused reflection of light, and surgery can be performed quickly. The present invention including this as one embodiment has been completed.
本開示は、以下の実施形態を包含する。
[１] 手術野用窓１１０を有する、動物に帝王切開手術を行うためのビニールアイソレータ。
[２] 手術野用窓１１０が、手術野用窓枠１２０及び両面粘着フィルム１１５を有する、実施形態１に記載のビニールアイソレータ。
[３] 両面粘着フィルム１１５が、透明軟質塩化ビニールである、実施形態２に記載のビニールアイソレータ。
[４] さらに、観察窓１３０を有する、実施形態１～３のいずれかに記載のビニールアイソレータ。
[５] さらに、連結部２１０を介して蘇生用ビニールアイソレータ２００に連結されている、実施形態１～４のいずれかに記載のビニールアイソレータ。
[６] 蘇生用ビニールアイソレータ２００が観察窓１３０を有する、実施形態５に記載のビニールアイソレータ。
[７] 観察窓１３０が、観察窓枠１３５及び硬質窓材料１４０を有する、実施形態４～６のいずれかに記載のビニールアイソレータ。
[８] 観察窓１３０の硬質窓材料１４０が透明硬質塩化ビニールである、実施形態７に記載のビニールアイソレータ。
[９] 実施形態１～８のいずれかに記載のビニールアイソレータを用いて、非ヒト動物３２０に帝王切開を行うことを含む、無菌動物の作出方法。
[１０] 帝王切開が、
(i)動物３２０の腹部を、動物手術用ビニールアイソレータ１００の手術野用窓１１０に密着させる工程、
(ii)動物手術用ビニールアイソレータ１００の内部から、メスを使用し、手術野用窓１１０に張られた両面粘着フィルム１１５ごと動物３２０の腹部を切開する工程、
(iii) 動物手術用ビニールアイソレータ１００の内側から、切開した両面粘着フィルム１１５及び動物３２０の部分をピンセットで挟んでクリップ止めにし、ビニールアイソレータ内の無菌状態が維持されるようにする工程、
(iv)切開した腹部から胎仔を動物手術用ビニールアイソレータ１００内へと摘出する工程、
を含む、実施形態９に記載の作出方法。
[１１] 前記工程(i)の前に、
(i-1)動物３２０の腹部の毛を予め剃っておく工程、
(i-2)毛を剃った動物の腹部３３０を消毒しておく工程、
をさらに含む、実施形態１０に記載の作出方法。
[１２] 前記動物３２０に帝王切開手術を行うためのビニールアイソレータ１００が、連結部２１０を介して、蘇生用ビニールアイソレータ２００に連結されており、さらに、
(v)摘出した胎仔を蘇生用ビニールアイソレータ２００に移し、蘇生用ビニールアイソレータ２００内で胎仔の表面を洗浄し、蘇生を行う工程、
を含む、実施形態１０又は１１に記載の作出方法。
The present disclosure includes the following embodiments.
[1] A vinyl isolator for performing caesarean section surgery on animals, having a surgical field window 110.
[2] The vinyl isolator according to Embodiment 1, wherein the surgical field window 110 has a surgical field window frame 120 and a double-sided adhesive film 115.
[3] The vinyl isolator according to Embodiment 2, wherein the double-sided adhesive film 115 is transparent soft vinyl chloride.
[4] The vinyl isolator according to any one of Embodiments 1 to 3, further comprising an observation window 130.
[5] The vinyl isolator according to any one of Embodiments 1 to 4, further connected to the resuscitation vinyl isolator 200 via a connecting portion 210.
[6] The vinyl isolator according to embodiment 5, wherein the vinyl isolator for resuscitation 200 has an observation window 130.
[7] The vinyl isolator according to any one of embodiments 4 to 6, wherein the observation window 130 has an observation window frame 135 and a hard window material 140.
[8] The vinyl isolator according to embodiment 7, wherein the hard window material 140 of the observation window 130 is transparent hard vinyl chloride.
[9] A method for producing a germ-free animal, comprising performing a cesarean section on a non-human animal 320 using the vinyl isolator according to any one of Embodiments 1 to 8.
[10] Caesarean section
(i) A step of bringing the abdomen of the animal 320 into close contact with the surgical field window 110 of the vinyl isolator 100 for animal surgery;
(ii) using a scalpel to incise the abdomen of the animal 320 along with the double-sided adhesive film 115 stretched over the surgical field window 110 from inside the animal surgical vinyl isolator 100;
(iii) From the inside of the vinyl isolator for animal surgery 100, the incised double-sided adhesive film 115 and the animal 320 are held together with tweezers and clipped to maintain the sterile state inside the vinyl isolator;
(iv) extracting the fetus from the incised abdomen into the vinyl isolator 100 for animal surgery;
The production method according to Embodiment 9, comprising:
[11] Before the step (i),
(i-1) a step of shaving the abdominal hair of the animal 320 in advance;
(i-2) a step of disinfecting the shaved animal's abdomen 330;
The production method according to Embodiment 10, further comprising:
[12] The vinyl isolator 100 for performing cesarean section surgery on the animal 320 is connected to the resuscitation vinyl isolator 200 via the connecting part 210, and further,
(v) a step of transferring the removed fetus to a resuscitation vinyl isolator 200, washing the surface of the fetus within the resuscitation vinyl isolator 200, and resuscitating it;
The production method according to Embodiment 10 or 11, comprising:
本発明の効果として、簡便に帝王切開法を動物に施すことができる。 As an effect of the present invention, a caesarean section can be easily performed on an animal.
本発明のビニールアイソレータ
ある実施形態において、本発明は、動物手術用ビニールアイソレータ１００を提供する。動物手術用ビニールアイソレータ１００は手術野用窓１１０を有する。本明細書において、手術野用窓１１０は、手術用の窓、手術用の枠、手術用窓又は単に手術窓ともいうことができるが、これらの用語は同義とする。手術野用窓１１０は、手術野用窓枠１２０を有し、手術野用窓枠１２０には両面粘着フィルム１１５を接着させる（張る）ことができる。すなわち、ある実施形態において、手術野用窓１１０は、手術野用窓枠１２０及び両面粘着フィルム１１５を有する。ある実施形態において、手術野用窓１１０は動物手術用ビニールアイソレータ１００の底面又は側面に設けることができる。これにより動物を、例えば動物手術用ビニールアイソレータ１００の底面（外界側の面）に密着させることができる。両面粘着フィルム１１５としては、例えば両面に粘着材料又は接着材料を有する透明軟質塩化ビニール（例えば0.02mm厚、日本ウエーブブロック株式会社製）が挙げられるが、これに限らない。両面粘着フィルム１１５、例えば透明軟質塩化ビニールは、0.01mm～1mm、0.02mm～0.5mm、例えば0.03mm、例えば0.02mm、例えば0.01mmの厚みを有するものであり得る。
Vinyl Isolators of the Invention In certain embodiments, the invention provides a vinyl isolator 100 for veterinary surgery. The vinyl isolator 100 for animal surgery has a surgical field window 110. In this specification, the surgical field window 110 can also be referred to as a surgical window, a surgical frame, a surgical window, or simply a surgical window, but these terms are synonymous. The surgical field window 110 has a surgical field window frame 120, and a double-sided adhesive film 115 can be adhered (pasted) to the surgical field window frame 120. That is, in one embodiment, the surgical field window 110 includes a surgical field window frame 120 and a double-sided adhesive film 115. In some embodiments, the surgical field window 110 can be provided on the bottom or side of the veterinary surgical vinyl isolator 100. This allows the animal to be brought into close contact with, for example, the bottom surface (the surface facing the outside world) of the vinyl isolator 100 for animal surgery. Examples of the double-sided adhesive film 115 include, but are not limited to, transparent soft vinyl chloride (for example, 0.02 mm thick, manufactured by Nippon Wave Block Co., Ltd.) having adhesive or adhesive materials on both sides. The double-sided adhesive film 115, such as transparent flexible vinyl chloride, may have a thickness of 0.01 mm to 1 mm, 0.02 mm to 0.5 mm, such as 0.03 mm, such as 0.02 mm, such as 0.01 mm.
動物手術用ビニールアイソレータ１００の寸法は、対象となる動物（実験動物又は家畜動物等）の大きさに応じて適宜、設計することができる。ある実施形態において、動物手術用ビニールアイソレータ１００は、例えば実験動物がマーモセット又はこれに類似する動物であれば、例えば500～5000cm、500～4000cm、500～3000cm、600～2500cm、700～2000cm、800～1500cm、例えば900～1200cmの長さ、例えば100～3000cm、150～2500cm、200～2000cm、250～1500cm、例えば300～1200cmの幅、例えば200～1000cm、300～900cm、400～800cm、500～700cm、例えば550～600cmの高さを有し得るが、寸法はこれに限らない。 The dimensions of the vinyl isolator 100 for animal surgery can be appropriately designed depending on the size of the target animal (laboratory animal, livestock animal, etc.). In some embodiments, the vinyl isolator 100 for animal surgery can be used, for example, when the experimental animal is a marmoset or a similar animal, for example, 500 to 5000 cm, 500 to 4000 cm, 500 to 3000 cm, 600 to 2500 cm, 700 to 2000 cm, or 800 cm. ~1500cm, e.g. 900-1200cm length, e.g. 100-3000cm, 150-2500cm, 200-2000cm, 250-1500cm, e.g. 300-1200cm width, e.g. 200-1000cm, 300-900cm, 400-800cm, 500- It may have a height of 700 cm, for example 550-600 cm, but the dimensions are not limited thereto.
別の実施形態において、本発明の動物手術用ビニールアイソレータ１００は、観察窓１３０を有することができる。本明細書において、特に断らない限り、観察窓１３０は手術野用窓１１０とは別の窓である。観察窓１３０は、観察窓枠１３５及び硬質窓材料１４０を有する。ある実施形態において、観察窓１３０は、硬質窓材料１４０として透明硬質塩化ビニール（0.1mm厚の薄い板）を、接着剤で、観察窓枠１３５に接着して構成する。硬質窓材料１４０は光の乱反射（拡散反射）の少ない材料であり得る。ある実施形態において、硬質窓材料１４０は透明塩化ビニール板であり得るがこれに限らない。本明細書において、乱反射が少ない、とは、ビニールアイソレータのような波打つことのできる軟質材料と比較して乱反射が少ないことをいい、例えば透明硬質塩化ビニールが示す程度に乱反射が少ないことをいう。また、本明細書において硬質窓材料１４０は光の乱反射が少ないことが好ましいが、このとき、硬質窓材料１４０が鏡面反射することは許容される。観察窓枠１３５に硬質窓材料１４０を取り付ける又は張ることにより観察窓１３０を構成することができる。ビニールアイソレータのような軟質材料と異なり、硬質窓材料１４０は波打たないため光の乱反射が少なく、手術のような細かい作業において、光の反射といった支障なく動物を観察でき、手術を短時間で遂行することができる。 In another embodiment, the veterinary surgical vinyl isolator 100 of the present invention can have a viewing window 130. In this specification, unless otherwise specified, the observation window 130 is a window different from the surgical field window 110. Viewing window 130 has a viewing window frame 135 and a rigid window material 140. In one embodiment, the observation window 130 is constructed by adhering a transparent hard vinyl chloride (thin plate with a thickness of 0.1 mm) as a hard window material 140 to the observation window frame 135 with an adhesive. The hard window material 140 may be a material that causes little diffused reflection of light. In some embodiments, the rigid window material 140 can be, but is not limited to, transparent PVC board. In this specification, "less diffused reflection" refers to less diffused reflection than that of a soft material that can be undulated, such as a vinyl isolator, and for example, means that the diffused reflection is as low as that exhibited by transparent hard vinyl chloride. Further, in this specification, it is preferable that the hard window material 140 has little diffused reflection of light, but at this time, specular reflection of the hard window material 140 is allowed. The observation window 130 can be constructed by attaching or stretching a hard window material 140 to the observation window frame 135. Unlike soft materials such as vinyl isolators, the hard window material 140 does not ripple and causes less diffuse reflection of light.In delicate operations such as surgeries, animals can be observed without problems such as light reflections, and operations can be completed in a shorter time. can be carried out.
ある実施形態において観察窓１３０及び観察窓枠１３５は円形、例えば略円形又は正円形とすることができる。なお、観察窓１３０及び観察窓枠１３５は必ずしも正円形である必要はなく、動物の形状や装置の使用態様に応じて、楕円形、四角形、長方形、正方形等とすることもできる。ある実施形態において、硬質窓材料１４０は硬質フィルム、例えばビニール板であり得る。観察窓１３０及び観察窓枠１３５は、ビニールアイソレータ越しに動物を観察し、帝王切開術を行うことができる程度の寸法であれば、どのような寸法でもよい。ある実施形態において観察窓枠１３５は、例えば5～50cm、10～40cm、例えば20～30cmの直径（内径）を有する円形であり得るがこれに限らない。ある実施形態において観察窓枠１３５は、例えば50cm2～8000cm2、300cm2～5000cm2、例えば1200cm2～3000cm2程度の面積（枠の内側の面積）を有する楕円形、四角形、長方形、又は正方形の枠であり得るがこれに限らない。本明細書において、円形の観察窓枠１３５のことを観察窓リングということがある。ある実施形態において、硬質窓材料１４０、例えばビニール板、例えば透明硬質塩化ビニール板は、例えば0.01mm～10mm、0.05mm～5mm、0.1mm～3mm、例えば約0.3mm、例えば約0.2mm、例えば約0.1mmの厚みを有するものであり得る。 In some embodiments, the viewing window 130 and the viewing window frame 135 can be circular, for example approximately circular or perfectly circular. Note that the observation window 130 and the observation window frame 135 do not necessarily have to be perfectly circular, but can also be oval, quadrilateral, rectangular, square, etc. depending on the shape of the animal and how the device is used. In some embodiments, rigid window material 140 can be a rigid film, such as a vinyl board. The viewing window 130 and the viewing window frame 135 may have any size as long as they allow the animal to be viewed through the vinyl isolator and a caesarean section to be performed. In some embodiments, the observation window frame 135 may be circular, with a diameter (inner diameter) of, for example, 5 to 50 cm, 10 to 40 cm, eg, 20 to 30 cm, but is not limited thereto. In one embodiment, the observation window frame 135 is an oval, square, rectangle, or square having an area (inside area of the frame) of , for example, 50 cm 2 to 8000 cm 2 , 300 cm 2 to 5000 cm 2 , for example 1200 cm 2 to 3000 cm 2 . However, it is not limited to this frame. In this specification, the circular observation window frame 135 is sometimes referred to as an observation window ring. In certain embodiments, the rigid window material 140, such as a vinyl board, such as a transparent rigid vinyl chloride board, has a diameter of, for example, 0.01 mm to 10 mm, 0.05 mm to 5 mm, 0.1 mm to 3 mm, such as about 0.3 mm, such as about 0.2 mm, such as about It may have a thickness of 0.1 mm.
ある実施形態において、動物手術用ビニールアイソレータ１００は、観察窓１３０を１以上、例えば２つ、３つ、又は４つ有し得る。ある実施形態において、観察窓１３０は動物手術用ビニールアイソレータ１００の上部（上面）及び／又は側部（側面）に設けることができる。別の実施形態では、動物手術用ビニールアイソレータ１００の上部及び側部の一部を傾斜させ、該傾斜面（傾斜部ともいう）に観察窓１３０を設けることができる。別の実施形態では、観察窓１３０は、動物手術用ビニールアイソレータ１００の上部、側部、及び／又は傾斜部に設けることができる。それぞれの観察窓１３０の寸法、形状、及び材料は、同一でも異なってもよい。 In some embodiments, the veterinary surgical vinyl isolator 100 may have one or more viewing windows 130, such as two, three, or four. In some embodiments, the viewing window 130 can be provided on the top and/or side of the veterinary surgical vinyl isolator 100. In another embodiment, a portion of the top and side portions of the vinyl isolator 100 for veterinary surgery may be sloped, and the observation window 130 may be provided on the sloped surface (also referred to as the sloped portion). In another embodiment, the viewing window 130 can be provided on the top, side, and/or slope of the veterinary surgical vinyl isolator 100. The size, shape, and material of each viewing window 130 may be the same or different.
ある実施形態において、本発明の動物手術用ビニールアイソレータ１００は、さらに蘇生用ビニールアイソレータ２００をその構成の一部として有しうる。別の実施形態において、本発明の動物手術用ビニールアイソレータ１００は、蘇生用ビニールアイソレータ２００に連結するための、連結部２１０を有しうる。連結部２１０を介して、動物手術用ビニールアイソレータ１００を蘇生用ビニールアイソレータ２００に連結し得る。ある実施形態において、蘇生用ビニールアイソレータ２００は、観察窓１３０を有する。観察窓１３０は、観察窓枠１３５及び硬質窓材料１４０を有する。観察窓枠１３５及び硬質窓材料１４０は、上述のものとすることができる。 In some embodiments, the veterinary surgical vinyl isolator 100 of the present invention may further include a resuscitation vinyl isolator 200 as part of its configuration. In another embodiment, the veterinary surgical vinyl isolator 100 of the present invention can have a coupling portion 210 for coupling to the resuscitation vinyl isolator 200. The veterinary surgical vinyl isolator 100 may be coupled to the resuscitation vinyl isolator 200 via the connection 210 . In some embodiments, the resuscitation vinyl isolator 200 has a viewing window 130. Viewing window 130 has a viewing window frame 135 and a rigid window material 140. Viewing window frame 135 and rigid window material 140 may be as described above.
ある実施形態において、動物を動物手術用ビニールアイソレータ１００の底面（外界側の面）に密着させるために、動物を載せる台を使用することができる。本明細書において、このような動物を載せる台を手術台３００、或いは帝王切開用保定台ということがある。手術台３００は高さを調節することのできる機構、例えばジャッキ３１０を有し得る。ジャッキ３１０は動物の大きさに応じて、ネジ式又は油圧式とすることができる。ジャッキ３１０としてはパンタグラフジャッキ、フロアジャッキ、折りたたみ式ジャッキが挙げられるがこれに限らない。 In some embodiments, a table on which the animal is placed can be used to bring the animal into close contact with the bottom surface (the outside surface) of the animal surgical vinyl isolator 100. In this specification, such a table on which an animal is placed is sometimes referred to as an operating table 300 or a caesarean section holding table. The operating table 300 may have a height adjustable mechanism, such as a jack 310. Jack 310 can be screw-type or hydraulic-type, depending on the size of the animal. Examples of the jack 310 include, but are not limited to, a pantograph jack, a floor jack, and a folding jack.
ある実施形態において、本発明の動物手術用ビニールアイソレータ１００は、アイソレータ内で帝王切開術を行うための、手袋１５０を取り付けうる。手袋１５０は２以上、例えば２つ、３つ、４つ、５つ、６つ、７つ、例えば８つ取り付けることができるがこれに限らない。本発明の動物手術用ビニールアイソレータ１００は、ビニールアイソレータが有する通常の構成、例えば搬入及び搬出を行う開閉部、無菌空気を送り込む吸気部及び空気を送り出す排気部を有しうる。搬入及び搬出を行う開閉部は、蘇生用ビニールアイソレータとの連結部を兼ねることができる。開閉部は１以上あってもよい。 In certain embodiments, the veterinary surgical vinyl isolator 100 of the present invention can be fitted with a glove 150 for performing a cesarean section within the isolator. Two or more gloves 150 can be attached, for example, two, three, four, five, six, seven, for example eight, but are not limited thereto. The vinyl isolator 100 for veterinary surgery of the present invention may have the usual configuration of a vinyl isolator, such as an opening/closing section for loading and unloading, an intake section for supplying sterile air, and an exhaust section for discharging air. The opening/closing part for loading and unloading can also serve as a connecting part with the resuscitation vinyl isolator. There may be one or more opening/closing parts.
帝王切開による無菌動物の作出方法
ある実施形態において、本発明の動物手術用ビニールアイソレータ１００を用いて、非ヒト動物３２０に帝王切開を行うことを含む、無菌動物の作出方法が提供される。この作出方法では、動物手術用ビニールアイソレータ１００の内部に、滅菌済みの手術器具を一式、配置しておくことができる。また、例えば動物３２０が体毛を有する場合などは、動物の腹部（腹面）は予め毛を剃っておくことができる（工程(i-1)）。また、毛を剃った動物の腹部３３０は消毒しておくことができる（工程(i-2)）。また、動物の体表は予め消毒しておいてもよい。動物が体毛を有しない場合はこれらの工程は省略し得る。次に、動物３２０を手術台３００に載せ、高さを調節する等して、動物３２０の腹部を、動物手術用ビニールアイソレータ１００の手術野用窓１１０に密着させることができる（工程(i)）。また、動物３２０には予め麻酔を施すことができる。次に、動物手術用ビニールアイソレータ１００の内部から、メスを使用し、手術野用窓１１０に張られた両面粘着フィルム１１５ごと動物３２０の腹部を切開する（工程(ii)）。本明細書において、両面粘着フィルム１１５ごと動物３２０の腹部を切開する、とは、両面粘着フィルム１１５と動物腹部を一緒に切開することをいう。切開するとき、動物腹部を両面粘着フィルム１１５に密着させたままにし、動物手術用ビニールアイソレータ１００の外部の空気が動物手術用ビニールアイソレータ１００内に入らないように操作する。次に、動物手術用ビニールアイソレータ１００の内側から、切った両面粘着フィルム１１５及び動物３２０の部分をピンセットで挟んでクリップ止めにし、ビニールアイソレータ内の無菌状態が維持されるようにする（工程(iii)）。次に切開した腹部から胎仔を動物手術用ビニールアイソレータ１００内へと摘出する（工程(iv)）。場合により、摘出した胎仔（新生仔）を蘇生用ビニールアイソレータ２００に移し、蘇生用ビニールアイソレータ２００内で胎仔（新生仔）の表面を洗浄するとともに蘇生を行うことができる（工程v）。蘇生後の胎仔（新生仔）は、例えば無菌環境等、適切な期間、飼育することができる。飼育には、例えば人工哺乳を使用し得る。
Method for Producing Germ-Free Animals by Cesarean Section In one embodiment, a method for producing germ-free animals is provided that includes performing a cesarean section on a non-human animal 320 using the animal surgical vinyl isolator 100 of the present invention. In this production method, a set of sterilized surgical instruments can be placed inside the vinyl isolator 100 for animal surgery. Further, for example, if the animal 320 has body hair, the animal's abdomen (ventral surface) can be shaved in advance (step (i-1)). Further, the shaved animal's abdomen 330 can be disinfected (step (i-2)). Further, the animal's body surface may be disinfected in advance. These steps can be omitted if the animal has no body hair. Next, the animal 320 is placed on the operating table 300 and the height is adjusted to bring the abdomen of the animal 320 into close contact with the surgical field window 110 of the animal surgical vinyl isolator 100 (step (i) ). Additionally, the animal 320 can be anesthetized in advance. Next, from inside the animal surgical vinyl isolator 100, the abdomen of the animal 320 is incised using a scalpel, including the double-sided adhesive film 115 stretched over the surgical field window 110 (step (ii)). In this specification, incising the abdomen of the animal 320 along with the double-sided adhesive film 115 means incising the double-sided adhesive film 115 and the animal's abdomen together. When making the incision, the animal's abdomen is kept in close contact with the double-sided adhesive film 115, and the operation is performed so that air outside the animal surgical vinyl isolator 100 does not enter into the animal surgical vinyl isolator 100. Next, from inside the vinyl isolator for animal surgery 100, the cut double-sided adhesive film 115 and the part of the animal 320 are held with tweezers and clipped to maintain the sterile state inside the vinyl isolator (step (iii) )). Next, the fetus is removed from the incised abdomen into the vinyl isolator 100 for animal surgery (step (iv)). In some cases, the removed fetus (newborn) can be transferred to the resuscitation vinyl isolator 200, and the surface of the fetus (newborn) can be washed and resuscitated within the resuscitation vinyl isolator 200 (step v). The resuscitated fetus (newborn) can be kept for an appropriate period of time, such as in a sterile environment. For example, artificial feeding can be used for breeding.
動物の子宮内は、基本的に無菌的である。しかしながら腸には通常、微生物が存在する。切開時に腸を傷つけると汚染が生じやすいため、好ましくは腸は傷つけないように切開操作を行う。また、腹部切開及び胎仔摘出の後、切開部を縫合するなどして母胎が早期に回復する処置を取り得る。回復した母胎は、子宮が摘出されていないため、再度の出産が可能であり、同じ母胎からの複数回の出産が可能となる。 The inside of an animal's uterus is basically sterile. However, microorganisms are normally present in the intestines. If the intestines are damaged during incision, contamination is likely to occur, so the incision is preferably performed without damaging the intestines. Furthermore, after abdominal incision and fetus removal, measures can be taken to quickly recover the mother's fetus, such as by suturing the incision. Since the uterus has not been removed from the recovered mother, it is possible to give birth again, making it possible to give birth multiple times from the same mother.
本発明の帝王切開方法、及び本発明の無菌動物の作出方法は、種々の動物に行うことができる。帝王切開を行う動物３２０は、例えば実験動物、家畜動物、野生動物、遺伝子操作動物、遺伝子組換え動物、クローン動物、ゲノム編集動物等であり得るがこれに限らない。また、ここでいう遺伝子操作動物、遺伝子組換え動物、クローン動物やゲノム編集動物は、第1世代のみならず、その子孫や系統を含み得るものとする。ある実施形態において、帝王切開を行う動物３２０は、哺乳動物であり得る。哺乳動物としては、マーモセット、マウス、ヌードマウス、ラット、ハムスター、モルモット、テンジクネズミ、フェレット、ウサギ、イヌ、ネコ、ブタ、ミニブタ、マイクロブタ、アカゲザル、カニクイザル、チンパンジー、ゴリラ、ウシ、ヒツジ、ヤギ、ウマ等が挙げられるがこれに限らない。本明細書において動物３２０という場合、ヒトは除かれるものとする。すなわち本明細書では非ヒト動物を、単に動物という。 The caesarean section method of the present invention and the method of producing germ-free animals of the present invention can be performed on various animals. The animal 320 on which the cesarean section is performed may be, for example, a laboratory animal, a domestic animal, a wild animal, a genetically modified animal, a genetically modified animal, a cloned animal, a genome edited animal, etc., but is not limited thereto. Furthermore, the genetically engineered animals, genetically modified animals, cloned animals, and genome edited animals referred to herein include not only the first generation but also their descendants and lines. In certain embodiments, the animal 320 performing the cesarean section can be a mammal. Mammals include marmosets, mice, nude mice, rats, hamsters, guinea pigs, guinea pigs, ferrets, rabbits, dogs, cats, pigs, mini pigs, micro pigs, rhesus monkeys, cynomolgus monkeys, chimpanzees, gorillas, cows, sheep, goats, Examples include, but are not limited to, horses. In this specification, when referring to animals 320, humans are excluded. That is, in this specification, non-human animals are simply referred to as animals.
本発明の動物手術用ビニールアイソレータにより、必ずしも無菌とは限らない動物から、無菌的に帝王切開術を行うことにより、無菌動物を作出することができる。また、作出される動物は、必ずしも完全に無菌状態である必要はない。例えば、所望により動物としてブタを使用し、本発明の動物手術用ビニールアイソレータにより、SPF（specific pathogen free）ブタを作出することもできる。本発明の動物手術用ビニールアイソレータにより、動物の子宮を切除することなく、胎仔を摘出することができる。また、本発明の動物手術用ビニールアイソレータにより、母胎を安楽死させることなく胎仔を摘出することができる。これは、従来の方法では帝王切開が難しかった動物については特に有利な効果である。すなわち本発明により動物の苦痛を緩和することができ、また、安楽死させる実験動物の数を削減することができる。これらは動物実験の3R理念に合致する、有利な効果をもたらす。 By using the vinyl isolator for animal surgery of the present invention, germ-free animals can be created from animals that are not necessarily sterile by performing a cesarean section aseptically. Furthermore, the animals produced do not necessarily need to be completely sterile. For example, if desired, a pig can be used as the animal, and SPF (specific pathogen free) pigs can be produced using the vinyl isolator for animal surgery of the present invention. With the vinyl isolator for animal surgery of the present invention, a fetus can be removed without removing the animal's uterus. Furthermore, the vinyl isolator for animal surgery of the present invention allows fetuses to be removed without euthanizing the mother's fetus. This is a particularly advantageous effect for animals for which caesarean sections are difficult to perform using conventional methods. That is, the present invention can alleviate animal suffering and reduce the number of experimental animals to be euthanized. These have beneficial effects that are consistent with the 3R philosophy of animal testing.
以下の実施例により本発明をさらに詳述する。下記の実施例は例証のみを目的としたものであり、何ら本発明を制限するものと解釈されてはならない。 The invention is further illustrated by the following examples. The following examples are for illustrative purposes only and are not to be construed as limiting the invention in any way.
[実施例１]
帝王切開による無菌マーモセットの作出
手術野用窓１１０を有する動物手術用ビニールアイソレータ１００を用いて、マーモセットに帝王切開術を行った。動物手術用ビニールアイソレータ１００の内部に、滅菌済みの手術器具を一式、配置した。マーモセットを麻酔し、その腹部（腹面）の毛を剃り、腹部を消毒した後、手術台３００に載せ、手術台３００の高さを調節し、腹部を、動物手術用ビニールアイソレータ１００の手術野用窓１１０に密着させた（図９）。次に、動物手術用ビニールアイソレータ１００の内部から、メスを使用し、手術野用窓１１０に張られた両面粘着フィルム１１５（0.02mm厚、日本ウエーブブロック株式会社製）ごとマーモセットの腹部を切開した。切開するとき、マーモセットの腹部を両面粘着フィルム１１５に密着させたままにし、動物手術用ビニールアイソレータ１００の外部の空気が動物手術用ビニールアイソレータ１００内に入らないように操作を行った。次に、動物手術用ビニールアイソレータ１００の内側から、切った両面粘着フィルム１１５及び動物３２０の部分をピンセットで挟んでクリップ止めにし、ビニールアイソレータ内の無菌状態が維持されるようにした。次に切開した腹部からマーモセットの仔を動物手術用ビニールアイソレータ１００内へと摘出した。その後、摘出したマーモセットの仔を蘇生用ビニールアイソレータ２００に移し、蘇生用ビニールアイソレータ２００内でマーモセットの仔の表面を洗浄するとともに蘇生を行った。蘇生後のマーモセットの仔は、ビニールアイソレータ内で飼育し、無菌であった。
[Example 1]
Creation of a sterile marmoset by caesarean section A caesarean section was performed on a marmoset using a vinyl isolator 100 for animal surgery having a window 110 for the surgical field. A set of sterilized surgical instruments was placed inside the vinyl isolator 100 for animal surgery. After anesthetizing the marmoset, shaving its abdomen (ventral surface), and disinfecting its abdomen, it is placed on the operating table 300, the height of the operating table 300 is adjusted, and its abdomen is placed in the surgical field of the vinyl isolator 100 for animal surgery. It was brought into close contact with the window 110 (FIG. 9). Next, the abdomen of the marmoset was incised from inside the vinyl isolator 100 for animal surgery using a scalpel, including the double-sided adhesive film 115 (0.02 mm thick, manufactured by Nippon Wave Block Co., Ltd.) stretched over the surgical field window 110. . When making the incision, the abdomen of the marmoset was kept in close contact with the double-sided adhesive film 115, and the operation was performed so that air outside the vinyl isolator for animal surgery 100 would not enter into the vinyl isolator for animal surgery 100. Next, from inside the vinyl isolator for animal surgery 100, the cut double-sided adhesive film 115 and the part of the animal 320 were held together with tweezers and secured with clips to maintain the sterile state inside the vinyl isolator. Next, the marmoset pup was removed from the abdominal incision into the vinyl isolator 100 for animal surgery. Thereafter, the extracted marmoset cub was transferred to a resuscitation vinyl isolator 200, and the surface of the marmoset cub was washed inside the resuscitation vinyl isolator 200 and resuscitated. After resuscitation, marmoset pups were kept in a vinyl isolator and kept sterile.
[実施例２]
手術野用窓１１０を有し、さらに観察窓１３０を有する動物手術用ビニールアイソレータ１００を使用し、上記と同じ手順でマーモセットに帝王切開術を行った。観察窓１３０は透明硬質塩化ビニール（0.1mm厚の薄い板）を接着剤で観察窓枠１３５に接着したものとした。観察窓１３０越しに、動物手術用ビニールアイソレータ１００内部を観察しながら、実施例１と同じ手順で帝王切開術を行った。摘出したマーモセットの仔を蘇生し、ビニールアイソレータ内で飼育したところ、無菌であった。
[Example 2]
Using a vinyl isolator 100 for animal surgery having a surgical field window 110 and an observation window 130, a cesarean section was performed on a marmoset in the same manner as described above. The observation window 130 was made of transparent hard vinyl chloride (a thin plate with a thickness of 0.1 mm) adhered to the observation window frame 135 with adhesive. A caesarean section was performed in the same manner as in Example 1 while observing the inside of the vinyl isolator 100 for animal surgery through the observation window 130. When the extracted marmoset pup was resuscitated and kept in a vinyl isolator, it was found to be sterile.
[比較例]
従来の無菌ブタ作出アイソレータ及び４週齢飼育アイソレータの写真を図１３及び１４に示す。また、従来の無菌ブタ作出アイソレータを用いた、子宮摘出術の写真を図１５に示す。まず、１．アイソレータを滅菌し、２．手術を開始する。次いで３．子宮を摘出し、４．これを過酢酸で洗浄する。その後、５．薬液槽を通し、６．蘇生を開始する。７．蘇生を行い、８．蘇生終了後、ブタ新生仔の９．飼育を行う。飼育は無菌ブタの場合、人工哺乳で４週齢まで行うのが一般的である。従来の子宮摘出では、母胎を安楽死せざるを得なかった。或いは、母胎は再度の妊娠及び出産が不可能であった。
[Comparative example]
Photos of a conventional sterile pig production isolator and a 4-week-old breeding isolator are shown in FIGS. 13 and 14. Furthermore, a photograph of a hysterectomy performed using a conventional sterile swine production isolator is shown in FIG. First, 1. Sterilize the isolator;2. Start the surgery. Then 3. Remove the uterus, 4. This is washed with peracetic acid. After that, 5. 6. Pass through the chemical bath. Begin resuscitation. 7. Perform resuscitation; 8. After resuscitation, 9. Carry out breeding. In the case of germ-free pigs, it is common to raise them by artificial feeding until they are 4 weeks old. With traditional hysterectomies, the mother's fetus had to be euthanized. Alternatively, the mother's fetus was unable to conceive and give birth again.
本発明により、動物の子宮を切除することなく、また、動物を安楽死させることなく、無菌動物や、特定の病原体を有しない動物（SPF動物）を作出することができる。 According to the present invention, germ-free animals and animals free of specific pathogens (SPF animals) can be produced without removing the animal's uterus or euthanizing the animal.
本明細書においては、特許出願、特許明細書、製造業者のマニュアルを含む文献が引用されることがある。これらの文書の開示は、本発明の特許性に関連するとはみなされないが、その全体を参照により本明細書に組み入れることとする。より詳細には、全ての参照文書を、個別の各文書が参照により組み入れられると具体的かつ個別に示されている場合と同様に、参照により本明細書に組み入れるものとする。 Documents, including patent applications, patent specifications, and manufacturer's manuals, may be cited herein. The disclosures of these documents are not considered relevant to the patentability of this invention, but are incorporated herein by reference in their entirety. More particularly, all referenced documents are herein incorporated by reference to the same extent as if each individual document was specifically and individually indicated to be incorporated by reference.
１００ 動物手術用ビニールアイソレータ
１１０ 手術野用窓
１１５ 両面粘着フィルム
１２０ 手術野用窓枠
１３０ 観察窓
１３５ 観察窓枠
１４０ 硬質窓材料
１５０ 手袋
２００ 蘇生用ビニールアイソレータ
２１０ 連結部
３００ 手術台
３１０ 手術台のジャッキ
３２０ 動物
３３０ 動物の毛を剃った腹部
４００ ４週齢飼育アイソレータ
５００ 無菌ブタ作出アイソレータ
100 Vinyl isolator for animal surgery 110 Window for surgical field 115 Double-sided adhesive film 120 Window frame for surgical field 130 Observation window 135 Observation window frame 140 Hard window material 150 Gloves 200 Vinyl isolator for resuscitation 210 Connection part 300 Operating table 310 Operating table jack 320 Animal 330 Shaved animal abdomen 400 4-week-old breeding isolator 500 Sterile pig production isolator
Claims (4)
手術野用窓１１０が、手術野用窓枠１２０及び両面粘着フィルム１１５を有し、
両面粘着フィルム１１５が、透明軟質塩化ビニールであり、
さらに、観察窓１３０を有する、
動物に帝王切開手術を行うためのビニールアイソレータであって、
観察窓１３０が、観察窓枠１３５及び硬質窓材料１４０を有し、
観察窓１３０の硬質窓材料１４０が透明硬質塩化ビニールである、
前記動物に帝王切開手術を行うためのビニールアイソレータ
を用いて、非ヒト動物３２０に帝王切開を行うことを含む、無菌動物の作出方法。 It has a surgical field window 110,
The surgical field window 110 has a surgical field window frame 120 and a double-sided adhesive film 115,
The double-sided adhesive film 115 is transparent soft vinyl chloride,
Furthermore, it has an observation window 130,
A vinyl isolator for performing cesarean section surgery on animals, the vinyl isolator comprising:
The viewing window 130 has a viewing window frame 135 and a rigid window material 140;
The hard window material 140 of the observation window 130 is transparent hard vinyl chloride.
A method for producing a germ-free animal comprising performing a cesarean section on a non-human animal 320 using a vinyl isolator for performing a cesarean section on the animal.
(i)非ヒト動物３２０の腹部を、動物手術用ビニールアイソレータ１００の手術野用窓１１０に密着させる工程、
(ii)動物手術用ビニールアイソレータ１００の内部から、メスを使用し、手術野用窓１１０に張られた両面粘着フィルム１１５ごと非ヒト動物３２０の腹部を切開する工程、
(iii)動物手術用ビニールアイソレータ１００の内側から、切開した両面粘着フィルム１１５及び非ヒト動物３２０の部分をピンセットで挟んでクリップ止めにし、ビニールアイソレータ内の無菌状態が維持されるようにする工程、及び、
(iv)切開した腹部から胎仔を動物手術用ビニールアイソレータ１００内へと摘出する工程、
を含む、請求項１に記載の作出方法。 A caesarean section is
(i) A step of bringing the abdomen of the non-human animal 320 into close contact with the surgical field window 110 of the vinyl isolator 100 for animal surgery;
(ii) using a scalpel to incise the abdomen of the non-human animal 320 along with the double-sided adhesive film 115 stretched over the surgical field window 110 from inside the animal surgical vinyl isolator 100;
(iii) A step of clipping the incised double-sided adhesive film 115 and the non-human animal 320 with tweezers from inside the animal surgical vinyl isolator 100 to maintain the sterile state inside the vinyl isolator; as well as,
(iv) extracting the fetus from the incised abdomen into the vinyl isolator 100 for animal surgery;
The production method according to claim 1 , comprising:
(i-1)非ヒト動物３２０の腹部の毛を予め剃っておく工程、
(i-2)毛を剃った動物の腹部３３０を消毒しておく工程、
をさらに含む、請求項２に記載の作出方法。 Before the step (i),
(i-1) a step of shaving the abdominal hair of the non-human animal 320 in advance;
(i-2) a step of disinfecting the shaved animal's abdomen 330;
The production method according to claim 2 , further comprising:
(v)摘出した胎仔を蘇生用ビニールアイソレータ２００に移し、蘇生用ビニールアイソレータ２００内で胎仔の表面を洗浄し、蘇生を行う工程、
を含む、請求項２又は３に記載の作出方法。 The vinyl isolator 100 for performing caesarean section surgery on the non-human animal 320 is connected to the resuscitation vinyl isolator 200 via the connecting part 210, and further,
(v) a step of transferring the removed fetus to a resuscitation vinyl isolator 200, washing the surface of the fetus within the resuscitation vinyl isolator 200, and resuscitating it;
The production method according to claim 2 or 3 , comprising:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020000742A JP7425468B2 (en) | 2020-01-07 | 2020-01-07 | Animal surgery vinyl isolator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020000742A JP7425468B2 (en) | 2020-01-07 | 2020-01-07 | Animal surgery vinyl isolator |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2021108772A JP2021108772A (en) | 2021-08-02 |
| JP7425468B2 true JP7425468B2 (en) | 2024-01-31 |
Family
ID=77058336
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020000742A Active JP7425468B2 (en) | 2020-01-07 | 2020-01-07 | Animal surgery vinyl isolator |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP7425468B2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114190285B (en) * | 2021-11-29 | 2023-03-31 | 华中农业大学 | Aseptic mouse preparation and integrated isolating device breeds |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020045796A1 (en) | 2000-10-17 | 2002-04-18 | O'connor Michael | Controlled environment device |
| WO2005092229A1 (en) | 2004-03-24 | 2005-10-06 | Davies, Christopher, Robert | Sterile enclosures |
| WO2018014003A1 (en) | 2016-07-15 | 2018-01-18 | Surgibox Inc. | Ultraportable system for intraoperative isolative and regulation of surgical site environments |
| JP2019115324A (en) | 2017-12-27 | 2019-07-18 | 澁谷工業株式会社 | Asepsis work system |
-
2020
- 2020-01-07 JP JP2020000742A patent/JP7425468B2/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020045796A1 (en) | 2000-10-17 | 2002-04-18 | O'connor Michael | Controlled environment device |
| WO2005092229A1 (en) | 2004-03-24 | 2005-10-06 | Davies, Christopher, Robert | Sterile enclosures |
| WO2018014003A1 (en) | 2016-07-15 | 2018-01-18 | Surgibox Inc. | Ultraportable system for intraoperative isolative and regulation of surgical site environments |
| JP2019115324A (en) | 2017-12-27 | 2019-07-18 | 澁谷工業株式会社 | Asepsis work system |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2021108772A (en) | 2021-08-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP7425468B2 (en) | Animal surgery vinyl isolator | |
| JP2009527293A (en) | Surgical isolation device | |
| Inzunza et al. | Germfree status of mice obtained by embryo transfer in an isolator environment | |
| US20090165723A1 (en) | Methods of producing avian eggs and birds of specified germ-free status | |
| CN214550377U (en) | Degassing unit is used in obstetrical nursing | |
| Püschel et al. | Rabbit mating and embryo isolation | |
| Jensen | Necropsy: a handbook and atlas | |
| CN211132261U (en) | Heater for operation | |
| Guerin et al. | A secure health status associated with the production and trade of in vitro derived cattle embryos | |
| CN213430583U (en) | Oral, maxillofacial and head-neck department concurrent hemorrhage exploration hemostasis operation bag | |
| CN215080153U (en) | Poultry slaughtering quarantine inspection operation panel | |
| Zubeidat et al. | Protocol for parotidectomy and saliva analysis in mice | |
| Vajta et al. | Production of Cloned Pigs by Handmade Cloning | |
| Lyons et al. | General considerations for mouse survival surgery | |
| CN208677583U (en) | Disposable uterine cavity spectroscopy combination packet | |
| CN218652125U (en) | Epididymis tail opening tool for mouse semen collection | |
| CN201631250U (en) | Soft biopsy forceps | |
| CN217310382U (en) | Quick sampling device for tissues of rat tail and rat tail | |
| CN218870867U (en) | Instrument disinfection device | |
| CN219763551U (en) | Storage box capable of sterilizing surgical knife | |
| CN210871953U (en) | Special midwifery device of gynaecology and obstetrics | |
| WO2024009796A1 (en) | Swine egg collection method and fertilized swine egg production method | |
| CN202537547U (en) | Biliary tract protection soft electrocoagulation biopsy forceps | |
| Yilmaz et al. | The treatment of congenitally developed fused vulva labia in a Brown-Swiss heifer | |
| Cai et al. | Caesarean Sectioning and Cross-Fostering of the Mouse |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20211119 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20220901 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20230524 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20230530 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20230721 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20230804 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20231031 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20231130 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20231201 |
|
| A911 | Transfer to examiner for re-examination before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20231220 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20240109 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20240112 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 7425468 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |