JP7366247B2 - 核酸検査装置および核酸検査方法 - Google Patents
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Description
[項目1]
標的核酸の標識物質および標的核酸の増幅試薬を含有する溶液が添加された組織切片(14)の温度を調節して組織切片(14)における核酸増幅反応を進行させ、
組織切片(14)の標識強度を経時的に検出し、
検出した情報を記憶することを含む核酸検査方法。
Claims (7)
- 標的核酸の標識物質および標的核酸の増幅試薬を含有する溶液が添加された組織切片が載置されるステージと、
前記ステージ上の前記組織切片の温度を調節する温度調節部と、
前記組織切片における核酸増幅反応を進行させるよう前記温度調節部を制御する温度制御部と、
前記組織切片の標識強度を経時的に検出する強度検出部と、
前記強度検出部が生成する検出情報を記憶する記憶部と、を備え、
さらに、前記組織切片の形状および標識強度の少なくとも一方に基づいて、前記検出情報上で前記組織切片中に検査領域を定める領域設定部、および使用者による前記検出情報上での前記組織切片中の検査領域の指定を受け付ける受付部の少なくとも一方と、
前記検査領域における前記標的核酸に関する情報を生成する情報処理部と、
を備え、
前記情報処理部は、前記検査領域の標識強度の経時変化を導出し、前記経時変化から前記検査領域における標的核酸の量を算出する核酸検査装置。 - 前記情報処理部は、前記検査領域における、標識強度が所定のしきい値以上である陽性領域と、標識強度が前記しきい値未満である陰性領域との比率を算出する請求項1に記載の核酸検査装置。
- 前記標的核酸は、所定の異常状態にある細胞に含まれ、
前記標識物質は、異常細胞を標識する第1標識物質と、正常細胞を標識する第2標識物質と、を含み、
前記情報処理部は、前記第1標識物質に起因する標識強度に基づいて前記陽性領域を特定し、前記第2標識物質に起因する標識強度に基づいて前記陰性領域を特定する請求項2に記載の核酸検査装置。 - 前記標的核酸は、所定の疾病に関連する核酸であり、
前記比率に基づいて前記疾病の重篤度を判定する判定部を備える請求項2または3に記載の核酸検査装置。 - 前記核酸増幅反応の進行中に前記標識強度が所定のしきい値を超える所定面積の領域が発生したことを検知する陽性検知部を備える請求項1乃至4のいずれか1項に記載の核酸検査装置。
- 前記情報処理部が生成する情報を表示する表示部を備える請求項1乃至4のいずれか1項に記載の核酸検査装置。
- 標的核酸の標識物質および標的核酸の増幅試薬を含有する溶液が添加された組織切片の温度を調節して前記組織切片における核酸増幅反応を進行させ、
前記組織切片の標識強度を経時的に検出し、
検出した情報を記憶することを含み、
さらに、前記組織切片の形状および標識強度の少なくとも一方に基づいて、前記検出した情報上で前記組織切片中に検査領域を定めること、および使用者による前記検出した情報上での前記組織切片中の検査領域の指定を受け付けることの少なくとも一方と、
前記検査領域における前記標的核酸に関する情報を生成することと、
を含み、
前記情報の生成は、前記検査領域の標識強度の経時変化を導出し、前記経時変化から前記検査領域における標的核酸の量を算出することを含む核酸検査方法。
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997032044A1 (en) | 1996-03-01 | 1997-09-04 | E.I. Du Pont De Nemours And Company | A method for the amplification and detection of a nucleic acid fragment of interest |
JP2009207392A (ja) | 2008-03-03 | 2009-09-17 | Olympus Corp | 増幅核酸の解析方法及び解析装置 |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997032044A1 (en) | 1996-03-01 | 1997-09-04 | E.I. Du Pont De Nemours And Company | A method for the amplification and detection of a nucleic acid fragment of interest |
JP2009207392A (ja) | 2008-03-03 | 2009-09-17 | Olympus Corp | 増幅核酸の解析方法及び解析装置 |
Non-Patent Citations (2)
Title |
---|
COMAR, M., et al.,"Direct in situ PCR allows rapid and sensitive detection of high risk human papillomavirus in cytologic specimens and formalin-fixed paraffin tissues by fluorescent labelling.",INTERNATIONAL JOURNAL OF ONCOLOGY,2001年,Vol.18, No.1,pp.181-185 |
青柳和子,「In situ PCR法の遺伝子への応用」,遺伝子医学,1998年04月01日,Vol.2, No.2,pp.282-286 |
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