JP7209079B2 - 細胞培養システム及び細胞培養方法 - Google Patents
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Description
次に、学習済みモデル59を得るための学習フェーズについて説明する。図4を参照して、本実施形態に係る情報処理装置12の学習フェーズにおける機能的な構成について説明する。図4に示すように、情報処理装置12は、取得部60、導出部62、及び生成部64を含む。CPU50が学習プログラム57を実行することにより、取得部60、導出部62、及び生成部64として機能する。
次に、学習済みモデル59を用いた運用フェーズについて説明する。運用フェーズは、各細胞培養チャンネル16にて細胞を培養するフェーズである。図8を参照して、本実施形態に係る情報処理装置12の運用フェーズにおける機能的な構成について説明する。図8に示すように、情報処理装置12は、取得部70、導出部72、及び統合制御部74を含む。CPU50が細胞培養プログラム58を実行することにより、取得部70、導出部72、及び統合制御部74として機能する。
複数の処理部を1つのプロセッサで構成する例としては、第1に、クライアント及びサーバ等のコンピュータに代表されるように、1つ以上のCPUとソフトウェアの組み合わせで1つのプロセッサを構成し、このプロセッサが複数の処理部として機能する形態がある。第2に、システムオンチップ(System on Chip:SoC)等に代表されるように、複数の処理部を含むシステム全体の機能を1つのIC(Integrated Circuit)チップで実現するプロセッサを使用する形態がある。このように、各種の処理部は、ハードウェア的な構造として、上記各種のプロセッサの1つ以上を用いて構成される。
12 情報処理装置
14 インキュベータ
16 細胞培養チャンネル
20 貯留部
22 ポンプ
24 流体デバイス
26 廃液部
28 流量測定部
30 微差圧測定部
32 pH測定部
34 抵抗測定部
36 撮影部
38、40 制御部
42 環境測定部
44 環境調整部
50 CPU
51 メモリ
52 記憶部
53 表示部
54 入力部
55 外部I/F
56 バス
57 学習プログラム
58 細胞培養プログラム
59 学習済みモデル
60、70 取得部
62、72 導出部
64 生成部
74 統合制御部
Claims (6)
- 細胞が培養される流体デバイス、液体を前記流体デバイスに流入させる送液部、前記流体デバイスで培養される細胞の状態を検出する第1センサ、及び前記送液部と前記第1センサとを制御する第1プロセッサをそれぞれ備えた複数の細胞培養チャンネルと、
前記複数の細胞培養チャンネルで培養されている細胞の培養環境を測定する第2センサと、
前記第1センサにより検出された細胞の状態及び前記第2センサにより測定された細胞の培養環境に基づいて、前記複数の前記細胞培養チャンネルそれぞれで培養される前記細胞の状態の評価値の差の絶対値が閾値以下になるように前記培養環境を調整可能な第2プロセッサと、
を備えた細胞培養システム。 - 前記第2プロセッサは、前記複数の細胞培養チャンネルで培養されている細胞の培養環境を細胞培養チャンネル毎に個別に調整する
請求項1に記載の細胞培養システム。 - 前記第2プロセッサは、前記細胞培養チャンネルの配置位置を表す配置位置情報及び前記細胞の状態から定まる前記細胞の状態の評価値を入力とし、前記培養環境を出力とした学習済みモデルを用いて前記培養環境を調整する
請求項1又は請求項2に記載の細胞培養システム。 - 前記細胞の状態は、前記細胞を撮影して得られた画像から導出される情報及び前記細胞の経上皮電気抵抗を含む
請求項1から請求項3の何れか1項に記載の細胞培養システム。 - 前記培養環境は、前記液体の流量、前記液体の温度、前記流体デバイスの周囲の環境温度、前記流体デバイスの周囲の環境湿度、前記流体デバイスの周囲の二酸化炭素濃度、前記流体デバイスの周囲の窒素濃度、及び前記流体デバイスの周囲の酸素濃度を含む
請求項1から請求項4の何れか1項に記載の細胞培養システム。 - 細胞が培養される流体デバイス、液体を前記流体デバイスに流入させる送液部、前記流体デバイスで培養される細胞の状態を検出する第1センサ、及び前記送液部と前記第1センサとを制御するプロセッサを備えた複数の細胞培養チャンネルを備えた細胞培養システムによる細胞培養方法であって、
前記複数の細胞培養チャンネルで培養されている細胞の培養環境を測定し、
前記第1センサにより検出された細胞の状態及び測定した細胞の培養環境に基づいて、前記複数の前記細胞培養チャンネルそれぞれで培養される前記細胞の状態の評価値の差の絶対値が閾値以下になるように前記培養環境を調整可能である
細胞培養方法。
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CN113388583A (zh) * | 2021-06-25 | 2021-09-14 | 北京理工大学 | 一种用于空间环境的片上原位细胞复苏、悬浮培养的方法及其微流控芯片和装置 |
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CN116407562A (zh) * | 2023-04-13 | 2023-07-11 | 安徽科门生物科技有限公司 | 脐带或胎盘或脐血来源间充质干细胞治疗慢阻肺应用 |
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JP2016054724A (ja) | 2014-09-12 | 2016-04-21 | 富士フイルム株式会社 | 細胞培養評価システムおよび方法 |
JP2018512853A (ja) | 2015-04-22 | 2018-05-24 | バークレー ライツ,インコーポレイテッド | マイクロ流体デバイス用の培養ステーション |
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US20210403847A1 (en) | 2021-12-30 |
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