JP7002915B2 - External skin preparation containing Saccharomyces yeast fermented liquid of collagen - Google Patents
External skin preparation containing Saccharomyces yeast fermented liquid of collagen Download PDFInfo
- Publication number
- JP7002915B2 JP7002915B2 JP2017211582A JP2017211582A JP7002915B2 JP 7002915 B2 JP7002915 B2 JP 7002915B2 JP 2017211582 A JP2017211582 A JP 2017211582A JP 2017211582 A JP2017211582 A JP 2017211582A JP 7002915 B2 JP7002915 B2 JP 7002915B2
- Authority
- JP
- Japan
- Prior art keywords
- collagen
- skin
- fermented liquid
- saccharomyces
- agents
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 102000008186 Collagen Human genes 0.000 title claims description 45
- 108010035532 Collagen Proteins 0.000 title claims description 45
- 229920001436 collagen Polymers 0.000 title claims description 45
- 239000007788 liquid Substances 0.000 title claims description 27
- 238000002360 preparation method Methods 0.000 title claims description 26
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims description 12
- 229930006000 Sucrose Natural products 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 238000000855 fermentation Methods 0.000 claims description 4
- 230000004151 fermentation Effects 0.000 claims description 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 2
- 239000002537 cosmetic Substances 0.000 description 18
- 239000000203 mixture Substances 0.000 description 15
- 239000003795 chemical substances by application Substances 0.000 description 14
- 239000000047 product Substances 0.000 description 12
- 238000000034 method Methods 0.000 description 10
- 230000003020 moisturizing effect Effects 0.000 description 8
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 7
- 238000007796 conventional method Methods 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 238000000605 extraction Methods 0.000 description 5
- 238000009472 formulation Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 241000235070 Saccharomyces Species 0.000 description 4
- 230000003110 anti-inflammatory effect Effects 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 239000004615 ingredient Substances 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 229930091371 Fructose Natural products 0.000 description 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 3
- 239000005715 Fructose Substances 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000002260 anti-inflammatory agent Substances 0.000 description 3
- 229940121363 anti-inflammatory agent Drugs 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 235000019688 fish Nutrition 0.000 description 3
- 239000006210 lotion Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 235000016068 Berberis vulgaris Nutrition 0.000 description 2
- 241000335053 Beta vulgaris Species 0.000 description 2
- 244000113306 Monascus purpureus Species 0.000 description 2
- 235000002322 Monascus purpureus Nutrition 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 241000235648 Pichia Species 0.000 description 2
- 241000198071 Saccharomyces cariocanus Species 0.000 description 2
- 241001123227 Saccharomyces pastorianus Species 0.000 description 2
- 241000235343 Saccharomycetales Species 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 150000002016 disaccharides Chemical class 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- -1 glucose or fructose Chemical class 0.000 description 2
- 235000021552 granulated sugar Nutrition 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000003779 hair growth Effects 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229940057059 monascus purpureus Drugs 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000013112 stability test Methods 0.000 description 2
- 150000003431 steroids Chemical class 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 1
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 description 1
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000238366 Cephalopoda Species 0.000 description 1
- 241000251730 Chondrichthyes Species 0.000 description 1
- 241000759833 Cornus officinalis Species 0.000 description 1
- 206010011985 Decubitus ulcer Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241001159781 Kazachstania spencerorum Species 0.000 description 1
- 241000039979 Kazachstania turicensis Species 0.000 description 1
- 241001123232 Kazachstania unispora Species 0.000 description 1
- 241000965982 Kazachstania zonata Species 0.000 description 1
- 241000235087 Lachancea kluyveri Species 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- 241001123224 Naumovozyma dairenensis Species 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000235072 Saccharomyces bayanus Species 0.000 description 1
- 241000877399 Saccharomyces chevalieri Species 0.000 description 1
- 241000877401 Saccharomyces ellipsoideus Species 0.000 description 1
- 241001407717 Saccharomyces norbensis Species 0.000 description 1
- 241001123228 Saccharomyces paradoxus Species 0.000 description 1
- 241000582914 Saccharomyces uvarum Species 0.000 description 1
- 241000242583 Scyphozoa Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 239000000730 antalgic agent Substances 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000000767 anti-ulcer Effects 0.000 description 1
- 230000001153 anti-wrinkle effect Effects 0.000 description 1
- 239000003429 antifungal agent Substances 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 239000003908 antipruritic agent Substances 0.000 description 1
- 239000003443 antiviral agent Substances 0.000 description 1
- 239000003212 astringent agent Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 239000002781 deodorant agent Substances 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003974 emollient agent Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 235000021107 fermented food Nutrition 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000013022 formulation composition Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000003349 gelling agent Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000036074 healthy skin Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 229960003444 immunosuppressant agent Drugs 0.000 description 1
- 239000003018 immunosuppressive agent Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000003410 keratolytic agent Substances 0.000 description 1
- 239000003589 local anesthetic agent Substances 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 229960002715 nicotine Drugs 0.000 description 1
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 238000006864 oxidative decomposition reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 229960005323 phenoxyethanol Drugs 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 229960002181 saccharomyces boulardii Drugs 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 210000004761 scalp Anatomy 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000003637 steroidlike Effects 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 150000004043 trisaccharides Chemical class 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 229920003169 water-soluble polymer Polymers 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Images
Landscapes
- Cosmetics (AREA)
Description
本発明は、皮膚外用剤、特に皮膚化粧料に有用な成分であるコラーゲンについて、従来知られている保湿効果、抗炎症効果などを損なうことなく、におい、呈色などの製剤上の問題を解決し、多量に配合することを可能にしたコラーゲン発酵液及びそれを配合した皮膚外用剤に関する。 INDUSTRIAL APPLICABILITY The present invention solves pharmaceutical problems such as odor and coloration of collagen, which is a useful ingredient for external skin preparations, especially skin cosmetics, without impairing the conventionally known moisturizing effect and anti-inflammatory effect. The present invention relates to a collagen fermented liquid that can be blended in a large amount and a skin external preparation containing the collagen fermented liquid.
コラーゲンは、三重らせん構造を有する分子量３０万程度のタンパク質であり、体内において、骨・軟骨・皮膚等の各細胞同士を結合する細胞外基質として広く知られている。特に、コラーゲンのもつ弾力性に注目した皮膚美容・化粧料の分野においては、加齢・紫外線等による外部刺激による減少・変質や体内への吸収、生成促進などを課題とした研究・開発が進められている。 Collagen is a protein having a triple helix structure and a molecular weight of about 300,000, and is widely known as an extracellular matrix that binds cells such as bone, cartilage, and skin to each other in the body. In particular, in the field of skin cosmetology and cosmetics, which focuses on the elasticity of collagen, research and development are proceeding with issues such as aging, reduction due to external stimuli such as ultraviolet rays, deterioration, absorption into the body, and promotion of production. Has been done.
一方で、コラーゲンは、その利用に際して、素材特性に依拠した課題を有している。すなわち、その巨大な分子量から皮膚・経口問わず吸収し難い点、溶解性に難があり製剤上不安定である点、特に動物系原料において顕著な独特の臭気や色を有する点などがよく知られている。 On the other hand, collagen has a problem depending on the material characteristics when it is used. In other words, it is well known that due to its huge molecular weight, it is difficult to absorb regardless of whether it is skin or oral, it is difficult to dissolve and it is unstable in the formulation, and it has a distinctive unique odor and color, especially in animal-based raw materials. Has been done.
これらの課題に対し、利用目的に応じて、物理・化学・生物的な手法をもって分解して分子量を小さくする方法、分子の一部を変性（化学修飾）して水性または／及び油性溶媒に対する溶解度を上げる方法、原料素材に対して前処理を施し脱色や臭気を軽減する方法などを用途等に応じて適宜採用している。 To solve these problems, depending on the purpose of use, a method of decomposing by physical, chemical, or biological methods to reduce the molecular weight, or a method of modifying (chemically modifying) a part of the molecule to make it soluble in an aqueous or / or oily solvent. A method of raising the temperature, a method of pretreating the raw material to reduce decolorization and odor, etc. are appropriately adopted according to the application.
しかしながら、非特許文献１の処方例でも明らかなように、皮膚外用剤、特に皮膚化粧料分野において利用する際の配合量には限度があり、１／３、１／２といった割合に至るような大量の配合を実現することはできていなかった。 However, as is clear from the prescription example of Non-Patent Document 1, there is a limit to the blending amount when used in the field of external skin preparations, especially in the field of skin cosmetics, and the ratio may reach 1/3 or 1/2. It was not possible to achieve a large amount of compounding.
ところで、皮膚外用剤、特に皮膚化粧料分野において、近年「発酵物」が特に注目されている。これは、微生物の働きかけにより素材に変化を生じせしめるもので、素材を分解させる方法、例えば、ヒアルロン酸など特定の成分物質や植物抽出物のような成分構成が一定ではない混合物を素材として、乳酸菌などの菌類や酵母などの広義の微生物の働きかけにより分解したものが知られている。 By the way, in recent years, "fermented products" have attracted particular attention in the field of external skin preparations, especially skin cosmetics. This is a method of decomposing the material by the action of microorganisms, for example, lactic acid bacteria using a specific component substance such as hyaluronic acid or a mixture such as a plant extract whose component composition is not constant. It is known that it is decomposed by the action of fungi such as yeast and microorganisms in a broad sense such as yeast.
このとき、微生物の生育に必要な炭素源、窒素源などの栄養源、その他の必要成分を添加した培養プロセスにより分解などの化学変化を促す方法が一般的であり、必要に応じて予備培養等の前処理もあわせて実施することもある。 At this time, a method of promoting chemical changes such as decomposition by a culture process in which nutrient sources such as carbon sources and nitrogen sources necessary for the growth of microorganisms and other necessary components are added is common, and pre-culture or the like is necessary. Pretreatment may also be carried out.
ところが、同じ素材であっても、微生物の種類、補充する栄養源その他の添加物や、温度、培養期間などの各要素によって、得られる生成物が異なること、そして、生成物は目的物と異なる夾雑物を含有すること、さらに培養の各要素を管理してさえ、その生成物の構成は変動しがちであり、一定とすることは難しいことから、抽出、分画その他の手法を併用し目的とする成分、または組成物を得ることが一般的である。 However, even if the same material is used, the obtained product differs depending on the type of microorganism, the nutrient source to be supplemented and other additives, the temperature, the culture period, and the like, and the product is different from the target product. Since the composition of the product tends to fluctuate and is difficult to keep constant even if it contains impurities and even if each element of the culture is managed, the purpose is to use extraction, fractionation and other methods together. It is common to obtain the ingredients or compositions to be used.
「発酵物」は、一般的には、このように微生物により素材を発酵させた生成物そのもの、または、ゴミや沈殿物をろ過する程度の軽度の後処理を経た複数成分からなる混合物または組成物を指しており、本発明においてもこの定義を援用する。
従って、「発酵物」は、素材、前処理を含む培養プロセス、後処理によって、おおよその成分構成は定まるものの、その本質は、不定成分が不定割合で存在する混合物であり、具体的に構成を特定することが難しい上に非現実的である。
A "fermented product" is generally the product itself, which is obtained by fermenting the material with microorganisms in this way, or a mixture or composition consisting of a plurality of components which has undergone a light post-treatment such as filtering dust or precipitate. This definition is also used in the present invention.
Therefore, although the "fermented product" has an approximate composition of components determined by the material, the culture process including pretreatment, and the post-treatment, the essence of the "fermented product" is a mixture in which indefinite components are present in an indefinite proportion. It is difficult and unrealistic to identify.
非特許文献１には、海藻の繊維分解酵素による分解液または酸化分解液に栄養源をあたえ、酵母または乳酸菌で発酵させた抽出液またはそれを乾燥させたものを配合した化粧料が記載されている。 Non-Patent Document 1 describes a cosmetic containing a nutrient source added to a decomposition solution or an oxidative decomposition solution of seaweed using a fibrous degrading enzyme, fermented with yeast or lactic acid bacteria, or a dried product thereof. There is.
非特許文献２には、ベニバナの花弁を熱水で抽出して固形分を除去して得られるベニバナ抽出エキスをそのままサッカロミケス目（Ｓａｃｃｈａｒｏｍｙｃｅｔａｌｅｓ）ピキア属（Ｐｉｃｈｉａ）ピキア・クドリアヴゼヴィイ（Ｐｉｃｈｉａ ｋｕｄｒｉａｖｚｅｖｉｉ）の酵母で発酵して得られる、線維芽細胞増殖用ベニバナ発酵液が記載されている。 In Non-Patent Document 2, the petals of Benibana are extracted with hot water to remove solids, and the extracted Benibana extract is used as it is in the order Saccharomycetales, Pichia, and Pichia kudriavzevi. ) Fermented Benibana fermented liquor for proliferation of fibroblasts, which is obtained by fermentation with yeast.
非特許文献３には、サンシュユ果実、コメヌカとベニコウジ菌を混合して発酵させた発酵液をプロテアーゼ処理して得られるコラーゲン産生作用を呈する組成物が記載されている。 Non-Patent Document 3 describes a composition exhibiting a collagen-producing action obtained by performing a protease treatment on a fermented liquid obtained by mixing and fermenting Cornus officinalis fruit, Monascus purpureus and Monascus purpureus.
非特許文献１には、当業者によって、様々な原料の配合に基づいた様々な剤型の皮膚外用剤の一種であるスキンケア・メイク化粧料組成物が提案されている。 In Non-Patent Document 1, a person skilled in the art proposes a skin care / make-up cosmetic composition which is a kind of skin external preparation of various dosage forms based on the combination of various raw materials.
しかしながら、皮膚外用剤、特に皮膚化粧料に有用な成分であるコラーゲンについて、従来知られている保湿効果、抗炎症効果などを損なうことなく、におい、呈色などの製剤上の問題を解決し、多量に配合することを可能にしたコラーゲン発酵液及びそれを配合した皮膚外用剤（本発明の効果・特徴）への応用可能性について記載または示唆されたものは存在しなかった。 However, collagen, which is a useful ingredient for external skin preparations, especially skin cosmetics, solves pharmaceutical problems such as odor and coloration without impairing the conventionally known moisturizing effect and anti-inflammatory effect. There was no description or suggestion of applicability to a collagen fermented liquid that could be blended in a large amount and a skin external preparation (effect / feature of the present invention) containing the collagen fermented liquid.
このような背景から、安定性及び安全性が良好で、長期間連続して使用することができ、従来知られている保湿効果、抗炎症効果などを損なうことなく、におい、呈色などの製剤上の問題を解決し、多量に配合可能なコラーゲン発酵液及びそれを配合した皮膚外用剤が求められていた。 Against this background, it has good stability and safety, can be used continuously for a long period of time, and is a formulation such as odor and coloration without impairing the conventionally known moisturizing effect and anti-inflammatory effect. There has been a demand for a collagen fermented liquid that can be blended in a large amount and a skin external preparation containing the collagen fermented liquid that solves the above problems.
発明者らは、コラーゲン類そのものではなく、これをサッカロミセス属酵母によって発酵させた培養液、好ましくは、培養培地に添加する糖類がショ糖及び／又は果糖を使用することで、安定性及び安全性が良好で、長期間連続して使用することができ、従来知られている保湿効果、抗炎症効果などを損なうことなく、におい、呈色などの製剤上の問題を解決し、さらに多量に配合可能なコラーゲン発酵液を配合した皮膚外用剤を得ることが出来ることに気づき発明を完成した。 The inventors have found that the sucrose and / or fructose is used as the saccharide added to the culture medium, preferably the culture medium obtained by fermenting the collagens with the yeast of the genus Saccharomyces, rather than the collagens themselves, for stability and safety. It is good and can be used continuously for a long period of time, solves formulation problems such as odor and coloration without impairing the conventionally known moisturizing effect, anti-inflammatory effect, etc. He realized that it was possible to obtain a skin external preparation containing a possible collagen fermented liquid, and completed the invention.
すなわち、本発明は以下の＜１＞から＜４＞に関する。
＜１＞サッカロミセス属酵母をコラーゲン類及び糖類を含有する培地で培養して得られる発酵液を含有することを特徴とする皮膚外用剤。
＜２＞糖類が、ショ糖及び／又は果糖であることを特徴とする請求項１に記載の皮膚外用剤。
＜３＞コラーゲン類が、コラーゲン、コラーゲンペプチド、加水分解コラーゲンからなる群の１つ以上からなることを特徴とする請求項１乃至請求項２に記載の皮膚外用剤。
＜４＞発酵液が、全体の５重量％以上８０重量％以下であることを特徴とする請求項１乃至請求項３に記載の皮膚外用剤。
なお、以下、これら本発明に含有される発酵液を「コラーゲン発酵液」と総称する。
That is, the present invention relates to the following <1> to <4>.
<1> An external skin preparation containing a fermented liquid obtained by culturing Saccharomyces yeast in a medium containing collagens and sugars.
<2> The external skin preparation according to claim 1, wherein the saccharide is sucrose and / or fructose.
<3> The external skin preparation according to claim 1 to 2, wherein the collagens are composed of one or more of the group consisting of collagen, collagen peptide, and hydrolyzed collagen.
<4> The external skin preparation according to claim 1 to 3, wherein the fermented liquid is 5% by weight or more and 80% by weight or less of the whole.
Hereinafter, the fermented liquids contained in the present invention are collectively referred to as "collagen fermented liquid".
本発明により、従来から知られているコラーゲン類の効果を減ずることなく、におい、呈色などの製剤上の問題点が解決され、多量に配合可能なコラーゲン発酵液、さらに、これを多量に配合した皮膚外用剤を提供することができる。 INDUSTRIAL APPLICABILITY According to the present invention, problems in formulation such as odor and color development are solved without reducing the effects of conventionally known collagens, and a collagen fermented liquid that can be blended in a large amount and further blended in a large amount. It is possible to provide an external preparation for skin.
本発明で発酵に用いるコラーゲンの起源、抽出方法又は加工・修飾手段等には特に制限はなく、起源としては、例えば、サメやサケ等の魚類、クラゲ類、イカなどの海洋生物由来のもの、豚、鶏などの動物由来のもの等が採用できる。抽出方法、加工・修飾手段についても、酸抽出によって得られる酸可溶化コラーゲン、ペプシン等の酵素処理によってテロペプチドを除去した酵素可溶化（アテロ化）コラーゲン、さらにはアシル化コラーゲンなど常法に従って抽出・加工・修飾を施して得られるコラーゲンのいずれをも採用することができる。 The origin, extraction method, processing / modifying means, etc. of collagen used for fermentation in the present invention are not particularly limited, and the origin includes, for example, fish such as sharks and salmon, and marine organisms such as jellyfish and squid. Animals derived from animals such as pigs and chickens can be used. Regarding the extraction method and processing / modification means, extraction is performed according to a conventional method such as acid-solubilized collagen obtained by acid extraction, enzyme-solubilized (atherotic) collagen obtained by removing telopeptides by enzymatic treatment such as pepsin, and acylated collagen. -Any of the collagens obtained by processing and modification can be adopted.
本発明で用いるサッカロミセス属酵母は代表的な出芽酵母であり、ビールやパンの発酵など、様々な発酵食品に利用されるのみならず、酵母の菌体自体も様々な栄養成分を含有する健康食品等として利用されている。知られている種としては、Saccharomyces bayanus、Saccharomyces boulardii、Saccharomyces bulderi、Saccharomyces cariocanus、Saccharomyces cariocus、Saccharomyces cerevisiae、Saccharomyces chevalieri、Saccharomyces dairenensis、Saccharomyces ellipsoideus、Saccharomyces florentinus、Saccharomyces kluyveri、Saccharomyces martiniae、Saccharomyces monacensis、Saccharomyces norbensis、Saccharomyces paradoxus、Saccharomyces pastorianus、Saccharomyces spencerorum、Saccharomyces turicensis、Saccharomyces unisporus、Saccharomyces uvarum、Saccharomyces zonatusなどがあり、本発明の実施にあたっては、これらのいずれか、または複数の組み合わせであってもよいが、特に「Saccharomyces cerevisiae」であることが望ましい。 The Saccharomyces yeast used in the present invention is a typical budding yeast, and is not only used in various fermented foods such as beer and bread fermentation, but also the yeast cells themselves are health foods containing various nutritional components. It is used as such. The species known, Saccharomyces bayanus, Saccharomyces boulardii, Saccharomyces bulderi, Saccharomyces cariocanus, Saccharomyces cariocus, Saccharomyces cerevisiae, Saccharomyces chevalieri, Saccharomyces dairenensis, Saccharomyces ellipsoideus, Saccharomyces florentinus, Saccharomyces kluyveri, Saccharomyces martiniae, Saccharomyces monacensis, Saccharomyces norbensis, There are Saccharomyces paradoxus, Saccharomyces pastorianus, Saccharomyces spencerorum, Saccharomyces turicensis, Saccharomyces unisporus, Saccharomyces uvarum, Saccharomyces zonatus, etc. It is desirable to be "cerevisiae".
本発明で用いる糖類は、発酵培養する酵母が栄養源として利用できるものであれば、ブドウ糖、果糖などの単糖類、ショ糖などの二糖類、であることが望ましい。また、さらに望ましくは、二糖類、最も望ましいのはショ糖である。但し、併用も差し支えなく、それは三糖類以上の多糖類であっても同様である。 The saccharide used in the present invention is preferably a monosaccharide such as glucose or fructose, or a disaccharide such as sucrose, as long as the yeast to be fermented and cultured can be used as a nutrient source. Further, more preferably, disaccharides, and most desirablely, sucrose. However, it does not matter if it is used in combination, and the same applies to polysaccharides of trisaccharides or higher.
本発明で用いるコラーゲン発酵液の調製は、前処理、後処理を含めて以下の通りである。なお、特に記載のない場合、百分率は重量百分率を意味しており、以降も同様である。
［コラーゲン発酵液の調製］
＜寒天培養＞
１．寒天培地を分注したプレートにサッカロミセス属酵母を播種する。
２．２８℃で３日間静置培養する。
＜前培養＞
１．ビートグラニュ糖、フィッシュコラーゲンペプチド、精製水からなる液体培地Ａを調製する。
２．プレートから菌液を回収し５３０ｎｍにおける濁度を測定し、当該濁度が０．５になるようフラスコ内で菌液を液体培地で希釈する。
３．２８℃、２００ｒｐｍで２４時間撹拌培養する。
＜本培養＞
１．ビートグラニュ糖、フィッシュコラーゲンペプチド、精製水からなる液体培地Ｂを調製する。
２．培養の菌液を液体培地Ｂに添加する。
３．２８℃、２００ｒｐｍで２４時間撹拌培養する。
＜滅菌＞
１．１２１℃、１５分間オートクレーブで滅菌する。
＜ろ過＞
１．ろ紙でろ過して固形物を取り除く。
２．０．４５μｍ、０．２２μｍのフィルターでろ過して固形物を取り除く。
（注）：１、３ブチレングリコール、フェノキシエタノールを添加して保存する。
The preparation of the collagen fermented liquid used in the present invention is as follows, including pretreatment and posttreatment. Unless otherwise specified, the percentage means the weight percentage, and the same applies hereinafter.
[Preparation of collagen fermented liquid]
<Agar culture>
1. 1. Saccharomyces yeast is inoculated on a plate dispensed with agar medium.
Incubate at 2.28 ° C. for 3 days.
<Preculture>
1. 1. A liquid medium A consisting of beet granulated sugar, fish collagen peptide, and purified water is prepared.
2. 2. The bacterial solution is collected from the plate, the turbidity at 530 nm is measured, and the bacterial solution is diluted with a liquid medium in a flask so that the turbidity becomes 0.5.
Incubate with stirring at 3.28 ° C. and 200 rpm for 24 hours.
<Main culture>
1. 1. A liquid medium B consisting of beet granulated sugar, fish collagen peptide, and purified water is prepared.
2. 2. The bacterial solution of the culture is added to the liquid medium B.
Incubate with stirring at 3.28 ° C. and 200 rpm for 24 hours.
<Sterilization>
1. Sterilize in an autoclave at 121 ° C for 15 minutes.
<Filtration>
1. 1. Filter with filter paper to remove solids.
Filter with a 2.0.45 μm, 0.22 μm filter to remove solids.
(Note): Add 1 to 3 butylene glycol and phenoxyethanol and store.
［外用剤の調製］
外用剤、例えば化粧料として調製する場合、パウダー、パウダーファンデーション等の粉体、石けん、リップスティック等の固体、ゲル（ジェル）、クリーム、乳液、リキッドファンデーション等の乳化物、化粧水、美容液等の液体など、種々の形態に調製することが出来る。また、シャンプー、トリートメント、頭皮用皮膚外用剤であってもよい。
[Preparation of external preparation]
When preparing as an external preparation, for example, powder, powder such as powder foundation, solid such as soap and lipstick, emulsion such as gel, cream, milky lotion, liquid foundation, cosmetic water, beauty essence, etc. It can be prepared in various forms such as the liquid of. It may also be a shampoo, a treatment, or an external skin preparation for the scalp.
この場合に配合し得る他の成分としては、通常、化粧品、医薬品等の外用剤の基剤成分あるいは添加剤成分または機能性を賦与する目的で用いられる成分、例えば、液状油分、固形油分、各種界面活性剤、金属セッケン、保湿剤、ゲル化剤、水溶性高分子、低級アルコール、多価アルコール、糖類、紫外線吸収剤、皮膜形成剤、樹脂、包接化合物、消臭剤、アミノ酸類、ビタミン類、薬剤、植物・動物・微生物由来の抽出物、有機酸、有機アミン、金属イオン封鎖剤、酸化防止剤、抗菌剤、防腐剤、ｐＨ調整剤、清涼剤、香料、エモリエント剤、色素、美白剤、抗しわ剤、抗老化剤、抗炎症剤、発毛剤、育毛促進剤、活性酸素除去剤、血行促進剤、収斂剤、角質溶解剤、タンパク質分解酵素などの成分、および外用薬の薬効成分としてのステロイド剤、非ステロイド剤を含む抗炎症剤、免疫抑制剤、鎮痛消炎剤、抗菌剤、抗真菌剤、抗ウイルス剤、抗腫瘍剤、抗潰瘍・褥瘡剤、創傷被覆剤、循環改善剤、止痒剤、局所麻酔剤、酔い止め剤、ニコチン剤、女性ホルモン剤等が挙げられる。調製にあたっては、本発明の実施に則した常法（例えば非特許文献１の調剤例）を用いることが出来る。 Other components that can be blended in this case are usually base components or additive components of external preparations such as cosmetics and pharmaceuticals, or components used for the purpose of imparting functionality, such as liquid oils, solid oils, and various types. Surface active agents, metal steroids, moisturizers, gelling agents, water-soluble polymers, lower alcohols, polyhydric alcohols, sugars, UV absorbers, film-forming agents, resins, inclusion compounds, deodorants, amino acids, vitamins Kinds, drugs, extracts from plants / animals / microorganisms, organic acids, organic amines, metal ion blockers, antioxidants, antibacterial agents, preservatives, pH adjusters, refreshing agents, fragrances, emollients, pigments, whitening Medicinal effects of agents, anti-wrinkle agents, anti-aging agents, anti-inflammatory agents, hair growth agents, hair growth promoters, active oxygen removers, blood circulation promoters, astringents, keratolytic agents, proteolytic enzymes, etc. Steroids as ingredients, anti-inflammatory agents including non-steroidal agents, immunosuppressants, analgesic and anti-inflammatory agents, antibacterial agents, antifungal agents, antiviral agents, antitumor agents, anti-ulcer / decubitus agents, wound covering agents, circulation improvement Examples thereof include agents, antipruritic agents, local anesthetic agents, anti-sickness agents, nicotine agents, female hormone agents and the like. In the preparation, a conventional method according to the practice of the present invention (for example, a dispensing example of Non-Patent Document 1) can be used.
上述の手順で調製したコラーゲン発酵液は、無色透明であり、わずかに溶媒のアルコール臭を有する他、特段の臭気を有しなかった。 The collagen fermented liquor prepared by the above procedure was colorless and transparent, had a slight alcoholic odor of the solvent, and had no particular odor.
［化粧水］
コラーゲン発酵液を使用して以下の構成成分を常法で調製した。
The following components were prepared by a conventional method using a collagen fermented liquor.
［ローション］
コラーゲン発酵液を使用して以下の構成成分を常法で調製した。
The following components were prepared by a conventional method using a collagen fermented liquor.
［クリーム剤］
コラーゲン発酵液を使用して以下の構成成分を常法で調製した。
The following components were prepared by a conventional method using a collagen fermented liquor.
［パック剤］
コラーゲン発酵液を使用して以下の構成成分を常法で調製した。
The following components were prepared by a conventional method using a collagen fermented liquor.
次に、コラーゲン発酵液の効果検証結果を示す。
検証にあたっては、モニター官能評価によるものとした。
具体的には、従業員から選抜した健常な皮膚を有する男女各３名のモニターを選出し、実施例２の化粧水処方構成をベースとするコラーゲン発酵液の配合量が５重量パーセントと８０重量パーセントのものの２種のサンプルを複数回使用させ、化粧料、特にスキンケア化粧料において訴求されコラーゲンの大量配合が求められる「保湿感」と大量配合によって低下する「使用感」について評価した。
このとき、「保湿感」は、使用前を含めて「肌のうるおい」「肌のなめらかさ」「肌のやわらかさ」を評価し、「使用感」は、「べたつき」「におい」について評価した。
評価は、「肌のうるおい」「肌のなめらかさ」「肌のやわらかさ」については「１．感じない、２．あまり感じない、３．どちらともいえない、４．やや感じる、５．感じる」の５段階評価、「べたつき」「におい」については「１．気にならない ２．あまり気にならない ３．どちらともいえない ４．やや気になる ５．気になる」の５段階で評価し、その平均値を採用した。
Next, the effect verification result of the collagen fermented liquid is shown.
The verification was based on monitor sensory evaluation.
Specifically, three monitors for each of men and women with healthy skin selected from employees were selected, and the amount of collagen fermented liquid based on the lotion formulation composition of Example 2 was 5% by weight and 80% by weight. Two kinds of samples of% were used multiple times, and the "moisturizing feeling" that was appealed in cosmetics, especially skin care cosmetics and required a large amount of collagen to be mixed, and the "feeling of use" that was reduced by the large amount of collagen were evaluated.
At this time, "moisturizing feeling" was evaluated for "skin moisture", "skin smoothness" and "skin softness" including before use, and "usability" was evaluated for "stickiness" and "smell". ..
The evaluation was "1. I don't feel, 2. I don't feel much, 3. I can't say either, 4. I feel a little, 5. I feel" about "moisturizing skin", "smoothness of skin", and "softness of skin". For "stickiness" and "smell", evaluate on a 5-point scale of "1. I don't care 2. I don't care much 3. I can't say either 4. I'm a little worried 5. I'm worried" The average value was adopted.
さらに、実施例２乃至４の調剤サンプル及びモニター評価に用いたサンプル２種について、４０°Ｃ・３ヶ月の安定性試験を実施したところ、
モニター評価サンプルの８０％配合品について、極微呈色が生じることが観察された。その後の進行が見られなかったこと、及び製品としての支障はないレベルであることから、ほぼ配合上の上限であることが示唆されるものの、充分な安定性を維持していることがわかる。
Furthermore, when the dispensing sample of Examples 2 to 4 and the two samples used for the monitor evaluation were subjected to a stability test at 40 ° C. for 3 months, the stability test was carried out.
It was observed that a slight coloration occurred in the 80% compounded product of the monitor evaluation sample. Since no further progress was observed and the level did not hinder the product, it is suggested that the upper limit of the formulation is almost the same, but it can be seen that sufficient stability is maintained.
本発明の皮膚外用剤は、外用化粧品としてメーキャップ化粧品やスキンケア化粧品（機能性化粧品）としてだけでなく、保湿効果をもつ機能性化粧品としても利用できる。また、薬事上の各種基準・要件を備えることによって、医薬部外品及び医薬品にも利用することができる。 The external skin preparation of the present invention can be used not only as makeup cosmetics and skin care cosmetics (functional cosmetics) as external cosmetics, but also as functional cosmetics having a moisturizing effect. In addition, it can be used for quasi-drugs and pharmaceuticals by providing various standards and requirements for pharmaceutical affairs.
Claims (1)
発酵液が、全体の５１重量％以上８０重量％以下である皮膚外用剤。 An external skin preparation containing a fermentation broth obtained by culturing Saccharomyces yeast in a medium containing collagen peptide and sucrose .
An external skin preparation containing 51% by weight or more and 80% by weight or less of the fermented liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2017211582A JP7002915B2 (en) | 2017-11-01 | 2017-11-01 | External skin preparation containing Saccharomyces yeast fermented liquid of collagen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2017211582A JP7002915B2 (en) | 2017-11-01 | 2017-11-01 | External skin preparation containing Saccharomyces yeast fermented liquid of collagen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2019085331A JP2019085331A (en) | 2019-06-06 |
| JP7002915B2 true JP7002915B2 (en) | 2022-01-20 |
Family
ID=66762278
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017211582A Active JP7002915B2 (en) | 2017-11-01 | 2017-11-01 | External skin preparation containing Saccharomyces yeast fermented liquid of collagen |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP7002915B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114748679A (en) * | 2022-03-27 | 2022-07-15 | 卢玉华 | Preparation method of collagen sponge |
| CN115074393B (en) * | 2022-08-04 | 2023-11-21 | 江苏创健医疗科技股份有限公司 | Pichia pastoris fermentation lysate filtrate, preparation method and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012087091A (en) | 2010-10-19 | 2012-05-10 | Kyoei Kagaku Kogyo Kk | Cosmetic |
| JP2015221756A (en) | 2014-05-22 | 2015-12-10 | 株式会社コーセー | Yeast culture and use thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0710734A (en) * | 1993-06-21 | 1995-01-13 | Dowa Mining Co Ltd | Cosmetic |
| KR20130114808A (en) * | 2012-04-10 | 2013-10-21 | 김정기 | Cosmetics composition including collagen natural fermented broth |
-
2017
- 2017-11-01 JP JP2017211582A patent/JP7002915B2/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012087091A (en) | 2010-10-19 | 2012-05-10 | Kyoei Kagaku Kogyo Kk | Cosmetic |
| JP2015221756A (en) | 2014-05-22 | 2015-12-10 | 株式会社コーセー | Yeast culture and use thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2019085331A (en) | 2019-06-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR20100135871A (en) | Active ingredient that stimulates the proliferation and/or activity of fibroblasts | |
| JP2004250372A (en) | Skin aging inhibitor/improver and/or rough skin inhibitor/improver kit | |
| JP2022049690A (en) | Novel aureobasidium pullulans strain and use thereof | |
| JPWO2017195873A1 (en) | Yeast Yarrowia lipolytica cells containing L-hydroxyproline or bacterial cell cultures or extracts thereof, uses thereof, and method for producing L-hydroxyproline | |
| JP7002915B2 (en) | External skin preparation containing Saccharomyces yeast fermented liquid of collagen | |
| KR20130114808A (en) | Cosmetics composition including collagen natural fermented broth | |
| EP2763652B1 (en) | Use of glucans obtained from prunus persica as an anti-aging cosmetic agent | |
| CN108451806B (en) | Natural anti-allergy moisturizing composition and preparation method and application thereof | |
| KR101292274B1 (en) | Cosmetic composition for anti-wrinkle | |
| KR101648148B1 (en) | Cosmetic composition containing fermented graviola for improving anti-wrinkle and method of preparing the same | |
| KR101985381B1 (en) | Cosmetic composition for exfoliating skin keratin comprising fruit fermentation complex made by fermenting grape, orange, apple, lemon and lime | |
| JP7222503B2 (en) | Skin topical agent | |
| KR20170128726A (en) | Cosmetics composition for anti-aging comprising agar-derived neoagarotetraose | |
| CN111956707A (en) | Method for improving growth speed of saccharomyces cerevisiae and content of effective components in plants by using red light | |
| KR101587077B1 (en) | Composition containing fermentated opuntia humifusa showing biological activity of skin | |
| US20240207169A1 (en) | Composition for improving skin wrinkle, moisturizing, strengthening skin barrier, and promoting skin regeneration, comprising mixed lactic acid bacteria as active ingredient | |
| US20160008252A1 (en) | Cosmetic use of queuine | |
| JPH05306213A (en) | Cosmetic | |
| JP5366358B2 (en) | Agent for acting on skin aging mechanism, anti-aging skin external preparation, and anti-aging method | |
| JP2021187787A (en) | Exosome secretagogue, exosome uptake promoter, exosome secretion-promoting beauty composition, and exosome secretion promoting method | |
| JP2021127323A (en) | HAS3 mRNA expression promoter | |
| JP7333669B2 (en) | Skin topical agent | |
| KR20090026426A (en) | Composition for moisturizing and improving wrinkle on skin | |
| KR20160122379A (en) | Manufacture method of fermented complex using dongchimi materials and cosmetic composition using by the method and cosmetic comprising the cosmetic composition | |
| CN111184822A (en) | Application of cogongrass rhizome fermentation product in improving gene expression quantity, promoting proliferation of collagen and elastin and improving oxidation resistance |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20201009 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20210630 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20210825 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20211217 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20211228 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 7002915 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313531 |
|
| S533 | Written request for registration of change of name |
Free format text: JAPANESE INTERMEDIATE CODE: R313533 |