JP6896004B2 - 検体識別分取装置および検体識別分取方法 - Google Patents
検体識別分取装置および検体識別分取方法 Download PDFInfo
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Description
(1)液体に分散させた被測定対象である検体を識別し、識別結果に基づいて目的検体を分取する検体識別分取装置であって、
液体に分散させた検体を収容する検体収容部と、前記液体を前記検体収容部の底部に備えられた出口から下方に延設される流路に送液するための圧力制御部と、前記検体に光を照射するための光照射部と、前記検体の光情報を測定する光情報測定部と、前記光情報に基づいて前記検体が目的検体か非目的検体であるか否かを判定する判定部と有する識別手段と、
前記識別手段の流路と連通する流路を有し、前記目的検体を含む分取溶液を容器へ分取する分取ノズルと、前記分取ノズルの先端から排出された排液、または非目的検体や分取不可能と判定された検体を含む排液を吸引回収する吸引ノズルを有する排液回収部と、目的検体を含む分取溶液を回収する回収容器とを有する分取手段と、
前記分取ノズル及び前記回収容器の少なくとも一方を移動する移動手段と、
前記光情報測定部で測定された光情報に基づいて、前記分取ノズル及び/又は前記回収容器を相対的に移動させる制御手段と、を備え、
前記検体収容部の内部空間内に配された撹拌部材を有する撹拌手段をさらに備えることを特徴とする検体識別分取装置。
液体に分散させた検体を、前記液体の出口を底部に備える検体収容部内に収容する収容ステップと、
前記検体収容部の内部空間に配された撹拌部材を有する撹拌手段によって撹拌させた状態で、前記液体を流路に送液する送液ステップと、
検体に光を照射する照射ステップと、
前記検体の光情報を測定する測定ステップと、
前記光情報に基づいて、前記検体が目的検体か非目的検体であるか否かを判定する判定ステップと、
前記流路に連通する分取ノズルの先端から排出された排液、または非目的検体や分取不可能と判定された検体を含む排液を、吸引ノズルにて吸引回収する回収ステップと、
前記光情報に基づいて、前記分取ノズルの先端を回収容器内に挿入するように、前記分取ノズル及び/又は前記回収容器を相対的に移動させる制御ステップと、
前記分取ノズルの先端から排出された目的検体を含む分取溶液を、前記回収容器へ分取する分取ステップと、
を有することを特徴とする検体識別分取方法。
また、図1および図2において、白丸で示す検体Sは分取対象となる目的検体であり、黒丸で示す検体SRは廃棄対象となる非目的検体である。
例えば、三方弁である開放弁20における各弁の働きは、図2に示すように、圧縮機21へ繋がる弁は「N.C.」の常時閉の状態で、制御時に開とし、大気へ繋がる弁は「N.O.」の常時開の状態で、制御時に閉とし、またパイプ17に繋がる弁は「COM」の常時開とすることができる。しかし、その制御の方法は各種の態様を取り得るものであり、図2に示すおよび前述したような態様に、特に限定されるわけではない。また、前記したような三方弁制御によるシース液の逆流は、逆流方法の一例であって、これ以外にも、例えば、圧縮機21の制御により検体収容部11内の圧力を下げたり、またシース液の圧力を上げることでも逆流させることはでき、その方法は特に限定されるわけではなく、種々の態様を取り得る。
撹拌部材としては、前述した図1および2に示す実施形態におけるように、撹拌翼を有するものに限られるものではなく、図3(a)の上下方向に沿った断面図に示すような、外径が下方に向かって漸次拡大すると共に上下方向に沿って螺旋状に延設されるもの、図3(f)の斜視図に示すようなロッド状の先端側である下方側が分岐しているもの、図3(g)の斜視図に示すようなロッド状の先端部に中実の円錐部材を備えるもの、棒状、円盤状などが挙げられる。また、撹拌部は、支持軸13cを備えずに、磁力によって回転するものであってもよい。この場合、撹拌部材13aの内部や側面には磁石が設けられ、撹拌駆動部13bは磁場発生器である。
すなわち、図6および図7に示す実施形態の検体識別分取装置においては、検体収容部11の上部位置に、撹拌部材13aに接続される回転軸部13dを下側から支持するとともに、検体収容部11の内部空間の圧力調整のためのパイプ17を介して外部の圧力制御部65(図示せず)に連通する把持ブロック16aが設けられている。把持ブロック16aには、上下方向に延びる貫通孔が形成され、把持ブロック16aの貫通孔の下側開口部と検体収容部11の上側開口部とが連結される。検体収容部11はその上部端面がフランジ状の接合面を有している。そして、前記把持ブロック16aの下面に検体収容部11のフランジ状の接合面を、例えばOリング等のシール材28を介して気密に接合するものとされており、検体収容部11を例えばディスポーサブルな部材として着脱交換可能としている。なお、これらの接合面には、さらに必要に応じて、螺子嵌合構造、嵌合い溝構造などや、粘着剤、接着剤等を使用してその密着性を高める態様を採ることは可能である。
11 検体収容部
13 撹拌手段
13a 撹拌部材
14 分取ノズル
50 排液回収部
61 識別手段
62 分取手段
64 制御手段
65 圧力制御部
66 光照射部
67 光情報測定部
68 判定部
69 回収容器
Claims (10)
- 液体に分散させた被測定対象である検体を識別し、識別結果に基づいて目的検体を分取する検体識別分取装置であって、
検体を分散している液体を導入する検体収容部と、前記液体を前記検体収容部の底部に備えられた出口から下方に延設される流路に送液するための圧力制御部と、前記検体に光を照射するための光照射部と、前記検体の光情報を測定する光情報測定部と、前記光情報に基づいて前記検体が目的検体か非目的検体であるか否かを判定する判定部と有する識別手段と、
前記識別手段の流路と連通する流路を有し、前記目的検体を含む分取溶液を容器へ分取する分取ノズルと、前記分取ノズルの先端から排出された排液、または非目的検体や分取不可能と判定された検体を含む排液を吸引回収する吸引ノズルを有する排液回収部と、目的検体を含む分取溶液を回収する回収容器とを有する分取手段と、
前記分取ノズル及び前記回収容器の少なくとも一方を移動する移動手段と、
前記光情報測定部で測定された光情報に基づいて、前記分取ノズル及び/又は前記回収容器を相対的に移動させる制御手段と、
支持軸と撹拌部材とを有する撹拌手段と
を備え、
前記支持軸は、回転可能に、前記検体収容部の蓋と前記撹拌部材の上端部とを連結し、
前記撹拌部材は、前記検体収容部の内部空間内に上下方向に亘って配され、前記検体収容部内の前記液体に対して上下方向に亘って且つ部分的に浸漬し、前記液体を撹拌して前記検体の分散状態を維持し、上下方向に延びる回転軸から水平方向に延設される1つ以上の撹拌翼を有することを特徴とする検体識別分取装置。 - 前記撹拌部材は、上下方向に延びる回転軸を回転中心として回転することを特徴とする請求項1に記載の検体識別分取装置。
- 前記撹拌部材が回転した際の前記撹拌部材の底部の回転面積が前記検体収容部の出口面積より大きいことを特徴とする請求項1または2に記載の検体識別分取装置。
- 前記検体収容部の底部が、前記出口に向かって傾斜していることを特徴としている請求項1〜3のいずれか1項に記載の検体識別分取装置。
- 前記撹拌部材は前記検体収容部の内面と離間配置されることを特徴とする請求項1〜4のいずれか1項に記載の検体識別分取装置。
- 前記撹拌翼は、前記検体収容部の上部側から底部側に向かって、水平方向に沿った翼幅が漸次拡張される請求項1〜5のいずれか1項に記載の検体識別分取装置。
- 前記検体収容部の内部空間が密閉されている請求項1〜6のいずれか1項に記載の検体識別分取装置。
- 前記識別手段の流路と連通する流路を、前記検体収容部から導出される検体を含む液体を囲繞している検体を含まない液体により形成するシース流形成部をさらに有しており、前記検体を含まない液体を前記検体収容部の出口より逆流させて、前記検体収容部内の撹拌を行う構成をさらに有するものである請求項1〜7のいずれか記載の検体識別分取装置。
- 液体に分散させた被測定対象である検体を識別し、識別結果に基づいて目的検体を分取する検体識別分取方法であって、
前記液体の出口を底部に備える検体収容部内に、検体を分散している液体を導入する収容ステップと、
支持軸と撹拌部材とを有する撹拌手段によって撹拌させた前記液体を流路に送液する送液ステップと、
検体に光を照射する照射ステップと、
前記検体の光情報を測定する測定ステップと、
前記光情報に基づいて、前記検体が目的検体か非目的検体であるか否かを判定する判定ステップと、
前記流路に連通する分取ノズルの先端から排出された排液、または非目的検体や分取不可能と判定された検体を含む排液を、吸引ノズルにて吸引回収する回収ステップと、
前記光情報に基づいて、前記分取ノズルの先端を回収容器内に挿入するように、前記分取ノズル及び/又は前記回収容器を相対的に移動させる制御ステップと、
前記分取ノズルの先端から排出された目的検体を含む分取溶液を、前記回収容器へ分取する分取ステップと、
を有し、
前記支持軸は、回転可能に、前記検体収容部の蓋と前記撹拌部材の上端部とを連結し、
前記撹拌部材は、前記検体収容部の内部空間内に上下方向に亘って配され、前記検体収容部内の前記液体に対して上下方向に亘って且つ部分的に浸漬し、前記液体を撹拌して前記検体の分散状態を維持し、上下方向に延びる回転軸から水平方向に延設される1つ以上の撹拌翼を有することを特徴とする検体識別分取方法。 - 前記検体収容部の内部空間が密閉されている請求項9に記載の検体識別分取方法。
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