JP6877163B2 - Cultivation method in contaminated soil - Google Patents
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- JP6877163B2 JP6877163B2 JP2017018232A JP2017018232A JP6877163B2 JP 6877163 B2 JP6877163 B2 JP 6877163B2 JP 2017018232 A JP2017018232 A JP 2017018232A JP 2017018232 A JP2017018232 A JP 2017018232A JP 6877163 B2 JP6877163 B2 JP 6877163B2
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- 239000002689 soil Substances 0.000 title claims description 64
- 238000012364 cultivation method Methods 0.000 title claims description 9
- 239000007864 aqueous solution Substances 0.000 claims description 28
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 claims description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 238000011109 contamination Methods 0.000 claims description 11
- 238000005507 spraying Methods 0.000 claims description 9
- 238000009335 monocropping Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 239000000243 solution Substances 0.000 claims description 4
- 238000009331 sowing Methods 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 19
- 201000010099 disease Diseases 0.000 description 16
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 description 12
- 238000000034 method Methods 0.000 description 9
- 239000004155 Chlorine dioxide Substances 0.000 description 6
- 230000000844 anti-bacterial effect Effects 0.000 description 6
- 235000019398 chlorine dioxide Nutrition 0.000 description 6
- UKLNMMHNWFDKNT-UHFFFAOYSA-M sodium chlorite Chemical compound [Na+].[O-]Cl=O UKLNMMHNWFDKNT-UHFFFAOYSA-M 0.000 description 5
- 229960002218 sodium chlorite Drugs 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 244000052769 pathogen Species 0.000 description 4
- 239000005708 Sodium hypochlorite Substances 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 3
- KZBUYRJDOAKODT-UHFFFAOYSA-N Chlorine Chemical compound ClCl KZBUYRJDOAKODT-UHFFFAOYSA-N 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000000645 desinfectant Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004880 explosion Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- XTEGARKTQYYJKE-UHFFFAOYSA-N chloric acid Chemical compound OCl(=O)=O XTEGARKTQYYJKE-UHFFFAOYSA-N 0.000 description 1
- 229940005991 chloric acid Drugs 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000007886 mutagenicity Effects 0.000 description 1
- 231100000299 mutagenicity Toxicity 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Description
本発明は、連作障害となる発病土を、植物を栽培可能な状態に殺菌した後播種して栽培する方法に関する。 The present invention relates to a method of sterilizing a diseased soil that hinders continuous cropping so that a plant can be cultivated and then sowing and cultivating the soil.
病害は、主に土壌中に病害菌が繁殖することによって惹き起こされ、農作物の連作障害の一因ともなる。ひとたび病害が発生すると地力を回復させることは困難であるし、除菌剤を用いると、当該除菌剤の土壌への残留及び農作物への蓄積，並びに使用者の健康に対する悪影響が懸念される。 Diseases are mainly caused by the growth of disease-causing bacteria in soil and contribute to the continuous cropping disorder of crops. Once a disease occurs, it is difficult to restore the soil fertility, and if a disinfectant is used, there is concern that the disinfectant may remain in the soil, accumulate in crops, and adversely affect the health of the user.
そこで、発病土を除菌する方法として、濃度約１００，０００ｐｐｍの亜塩素酸ナトリウム水溶液を水により希釈し、濃度約１，０００ｐｐｍの亜塩素酸ナトリウムの水溶液を作り、この希釈液を土壌１０アール当り約２，０００〜２，５００Ｌの割合で撒布した後 、二酸化塩素ガスがほぼ消失するまで約５日乃至２週間放置し、土壌を乾燥させた後施肥し植物を栽培することを特徴とする植栽用土壌の改善方法が開示されている（例えば、特許文献１参照）。 Therefore, as a method for eradicating diseased soil, an aqueous solution of sodium chlorite having a concentration of about 100,000 ppm is diluted with water to prepare an aqueous solution of sodium chlorite having a concentration of about 1,000 ppm, and this diluted solution is used for 10 ares of soil. After sprinkling at a rate of about 2,000 to 2,500 L per unit, it is left for about 5 days to 2 weeks until the chlorine dioxide gas almost disappears, and after the soil is dried, fertilized plants are cultivated. A method for improving soil for planting is disclosed (see, for example, Patent Document 1).
しかしながら、上記方法は、亜塩素酸ナトリウムが土壌中で起こす化学反応を利用するものであり、それによって生じる二酸化塩素で土壌を除菌する工程を含む。二酸化塩素は反応性の高いラジカルであって、有毒であり、爆発の危険性を有する。しかも、二酸化塩素は、空気より重いから地上に滞留し易く、作業者の健康に甚大な悪影響を及ぼす虞がある。また、土壌に残留する未反応の亜塩素酸ナトリウムが、長期に亘って二酸化塩素を発生し続け、又は、土壌中で意図しない化学反応を起こす虞もある。亜塩素酸ナトリウムは変異原性を有するから、それを吸収した農作物及び当該農作物を摂食したヒトの遺伝子に 変異を起こすことも危惧される。 However, the above method utilizes a chemical reaction caused by sodium chlorite in the soil, and includes a step of sterilizing the soil with chlorine dioxide generated thereby. Chlorine dioxide is a highly reactive radical, toxic and has an explosion risk. Moreover, since chlorine dioxide is heavier than air, it tends to stay on the ground, which may have a great adverse effect on the health of workers. In addition, unreacted sodium chlorite remaining in the soil may continue to generate chlorine dioxide for a long period of time, or may cause an unintended chemical reaction in the soil. Since sodium chlorite has mutagenicity, there is a concern that it may cause mutations in the crops that have absorbed it and the genes of humans who have eaten the crops.
他方、そこで、強い殺菌力を持ち速やかに分解する次亜塩素酸に着目し、５０ｐｐｍ以上１００ｐｐｍ以下の次亜塩素酸ソーダ水溶液を土壌１０a当り５００〜１０００リットル散布した後、１乃至７日放置した後、施肥する土壌改良法が提案されている（例えば、 特許文献２）。 On the other hand, focusing on hypochlorous acid, which has strong bactericidal activity and rapidly decomposes, 500 to 1000 liters of sodium hypochlorite aqueous solution of 50 ppm or more and 100 ppm or less was sprayed per 10a of soil, and then left for 1 to 7 days. Later, a soil improvement method for fertilizing has been proposed (for example, Patent Document 2).
しかしながら、５０ｐｐｍ以上１００ｐｐｍ以下の次亜塩素酸ソーダ水溶液を土壌１０a当り５００〜１０００リットル散布する方法では、発病土の菌種によっては除菌の有効性は少なく、健全苗率が向上しない。例えば、コマツナ立ち枯れ病汚染土壌では５０ｐｐｍ以上１００ｐｐｍ以下の次亜塩素酸ソーダ水溶液を散布する方法では健全苗率が改善されないという結果を招来した。そこで、栽培方法としてはさらに検討する必要がある。これらの問題点に鑑み、本発明は、有効な栽培方法を提供することを課題とする。 However, in the method of spraying 500 to 1000 liters of sodium hypochlorite aqueous solution of 50 ppm or more and 100 ppm or less per 10a of soil, the effectiveness of sterilization is small and the healthy seedling rate is not improved depending on the bacterial species of the diseased soil. For example, in the soil contaminated with Komatsuna wilt disease, the method of spraying an aqueous solution of sodium hypochlorite of 50 ppm or more and 100 ppm or less did not improve the healthy seedling rate. Therefore, it is necessary to further study the cultivation method. In view of these problems, it is an object of the present invention to provide an effective cultivation method.
本発明者は、鋭意研究の結果、汚染度を調整して用いる混和発病土においては、直接高濃度の次亜塩素酸水溶液を土壌と接触させるのではなく、まず、土壌を一定の濃度に加水し、湿潤させ、混和発病土の汚染度を勘案してｐH５〜６．５の１０００以上６０００ｐｐｍ以下、好ましくは１５００〜４５００ｐｐｍの次亜塩素酸水溶液を散布すると、残留が問題とされやすい高濃度次亜塩素酸水溶液で直接処理するより健全苗率が向上することを見出し、本発明を完成した。 As a result of diligent research, the present inventor did not directly contact the soil with a high-concentration hypochlorous acid aqueous solution in the mixed diseased soil used by adjusting the degree of contamination, but first added water to a certain concentration. Then, when moistened and sprayed with an aqueous solution of hypochlorite having a pH of 5 to 6.5 of 1000 or more and 6000 ppm or less, preferably 1500 to 4500 ppm in consideration of the degree of contamination of the mixed soil, a high concentration order in which residue is likely to be a problem. The present invention has been completed by finding that the rate of healthy seedlings is improved as compared with direct treatment with an aqueous chloric acid solution.
すなわち、本発明は連作障害の汚染発病土での栽培方法であって、 予め発病土壌の湿潤度を考慮して加水し、湿潤させる工程と、その後、混和発病土にｐH５〜６．５の１０００以上６０００ｐｐｍ以下の次亜塩素酸水溶液を発病土の汚染度を考慮して土壌容量の２分の１から２倍量散布し、浸透させる工程と、１〜2週間遮光フィルムで覆い殺菌する工程と、その後播種する ことを特徴とする栽培方法にある。 That is, the present invention is a cultivation method in a contaminated soil of continuous cropping disorder , in which a step of adding water and moistening the soil in advance in consideration of the wetness of the soil, and then 1000 of pH 5 to 6.5 on the mixed soil. A step of spraying a hypochlorous acid aqueous solution of 6000 ppm or less in an amount of 1/2 to 2 times the soil volume in consideration of the degree of contamination of the diseased soil and infiltrating it, and a step of covering with a light-shielding film for 1 to 2 weeks to sterilize the soil. The cultivation method is characterized by sowing afterwards.
本発明においては、病原菌による連作障害を回避することができる。その作用は土中への弱酸性次亜塩素酸水溶液の病原菌の棲む土中への湿潤による浸透殺菌作用と、遮光フィ ルムで遮蔽による塩素ガス発生による殺菌作用であり、次亜塩素酸の残留もなく、安全で有効な連作障害を防止できる。発病土に対する加水度は病原菌の棲む土中への浸透率を考慮して予め発病土壌の湿潤度を考慮して加水し、湿潤させるのがよい。単なる水であってもよいが湿潤水としては５０〜１００ｐｐｍ以下の希薄次亜塩素酸水溶液を使用してもよい。次いで、散布する次亜塩素酸濃度はｐＨ５〜６．５の弱酸性領域で１０００〜６０００、好ましくは１５００〜４５００ｐｐｍの範囲で使用され、発病土の汚染度を考慮して決定し、土壌容量の２分の１から２倍容量を散布して必要領域を十分に湿潤させるのがよい。次亜塩素酸水溶液の病原菌との接触により殺菌するのが好ましい。散布後は遮光フィルムで散布箇所を覆い、遮蔽するのがよい。直射日光による次亜塩素酸水溶液の分解を避け、発生する塩素ガスを閉じ込め、高い殺菌効果を達成するためである。 因みに、本発明方法で育苗すると、健全苗率が向上する。コマツナ立ち枯れ病汚染土壌ではｐH６の３０００ｐｐｍ次亜塩素酸水溶液は散布前に加水しておくと、５０〜１００ｐｐｍの希釈液処理の場合より３倍ほど健全苗率が向上することが見出された。散布前の加水の及ぼすその後の次亜塩素酸水溶液の殺菌メカニズムについては定かでないが、高濃度 次亜塩素酸水溶液を直接散布するより大きな健全苗率が向上することはその後施肥して育苗する必要のある植物の育成方法としては利用価値が高い。 In the present invention, continuous cropping disorder due to pathogens can be avoided. Its actions are the permeation bactericidal action by wetting the soil with the pathogens of the weakly acidic hypochlorous acid aqueous solution into the soil, and the bactericidal action by generating chlorine gas by shielding with a light-shielding film, and the residual hypochlorous acid remains. It is possible to prevent safe and effective continuous cropping failure. As for the degree of water addition to the diseased soil, it is preferable to add water in advance in consideration of the wetness of the diseased soil in consideration of the penetration rate into the soil in which the pathogen inhabits. Although it may be simple water, a dilute hypochlorous acid aqueous solution of 50 to 100 ppm or less may be used as the moist water. Next, the concentration of hypochlorous acid to be sprayed is used in the weakly acidic region of pH 5 to 6.5 in the range of 1000 to 6000, preferably 1500 to 4500 ppm, and is determined in consideration of the degree of contamination of the diseased soil, and the soil volume is determined. It is advisable to spray 1/2 to 2 times the volume to sufficiently moisten the required area. It is preferable to sterilize by contacting the hypochlorous acid aqueous solution with pathogens. After spraying, it is advisable to cover the sprayed area with a light-shielding film to shield it. This is to avoid decomposition of the hypochlorous acid aqueous solution by direct sunlight, trap the generated chlorine gas, and achieve a high bactericidal effect. Incidentally, when seedlings are raised by the method of the present invention, the healthy seedling rate is improved. It was found that in soil contaminated with Komatsuna wilt disease, if a 3000 ppm hypochlorous acid aqueous solution having a pH of 6 was added before spraying, the healthy seedling rate was improved by about 3 times as compared with the case of a diluted solution treatment of 50 to 100 ppm. The sterilization mechanism of the subsequent hypochlorous acid aqueous solution affected by water before spraying is not clear, but it is necessary to fertilize and raise seedlings afterwards to improve the higher healthy seedling rate than direct spraying of high-concentration hypochlorous acid aqueous solution. It has high utility value as a method of growing plants with
以下、本発明を実施例に基づいて説明する。 Hereinafter, the present invention will be described based on examples.
コマツナ立ち枯れ病汚染土壌を用いて汚染度５％の発病土を用意する。実施例１ではこの汚染発病土１００ｇに対し１００ｇの蒸留水を散水して加水した後、混和発病土１００ｇ当りｐH６の３０００ｐｐｍの次亜塩素酸水溶液を２５ｍｌ散布し、１０日間遮光フィルムで覆い、その後１区１６粒播種して４日後の健全発病率を判定した。この試験を５回反復してその結果、平均７０．１％の健全苗率を得た（図１参照）。 Prepare soil with a degree of contamination of 5% using soil contaminated with Komatsuna wilt disease. In Example 1, 100 g of distilled water was sprinkled on 100 g of the contaminated diseased soil to add water, and then 25 ml of a 3000 ppm hypochlorous acid aqueous solution having a pH of 6 per 100 g of the mixed diseased soil was sprayed and covered with a light-shielding film for 10 days. The healthy incidence rate was determined 4 days after sowing 16 seeds in 1 group. This test was repeated 5 times, and as a result, an average healthy seedling rate of 70.1% was obtained (see FIG. 1).
ｐH６の３０００ｐｐｍの次亜塩素酸水溶液を５０ｍｌ散布する以外は実施例１と同様にして平均５０．６％の健全苗率を得た（図２参照）。 An average healthy seedling rate of 50.6% was obtained in the same manner as in Example 1 except that 50 ml of a 3000 ppm hypochlorous acid aqueous solution having a pH of 6 was sprayed (see FIG. 2).
ｐH６の３０００ｐｐｍの次亜塩素酸水溶液を６０ｍｌ散布する以外は実施例１と同様にして平均４５％の健全苗率を得た（図３参照）。 An average of 45% healthy seedling rate was obtained in the same manner as in Example 1 except that 60 ml of a 3000 ppm hypochlorous acid aqueous solution having a pH of 6 was sprayed (see FIG. 3).
コマツナ立ち枯れ病汚染土壌を用いて汚染度５％の発病土を用意する。この汚染発病土１００ｇに対し２５ｍｌ蒸留水を散水し、混和発病土１００ｇ当りｐH６の３０００ｐｐｍの次亜塩素酸水溶液を１００ｍｌ散布し、１０日間遮光フィルムで覆い、その後１区１６粒播種して４日後の健全発病率を判定した。この試験を５回反復してその結果、平均４ ８．７％の健全苗率を得た（図４参照）。 Prepare soil with a degree of contamination of 5% using soil contaminated with Komatsuna wilt disease. 25 ml of distilled water was sprinkled on 100 g of the contaminated diseased soil, 100 ml of a 3000 ppm hypochlorous acid aqueous solution of pH 6 was sprayed per 100 g of the mixed diseased soil, covered with a light-shielding film for 10 days, and then 16 seeds in 1 group were sown 4 days later. The healthy incidence of the disease was determined. This test was repeated 5 times, resulting in an average healthy seedling rate of 48.7% (see FIG. 4).
ｐH６の３０００ｐｐｍの次亜塩素酸水溶液を１２０ｍｌ散布する以外は実施例４と同様にして平均５２．１％の健全苗率を得た（図５参照）。
［比較例１］
An average healthy seedling rate of 52.1% was obtained in the same manner as in Example 4 except that 120 ml of a 3000 ppm hypochlorous acid aqueous solution having a pH of 6 was sprayed (see FIG. 5).
[Comparative Example 1]
コマツナ立ち枯れ病汚染土壌を用いて汚染度５％の発病土を用意する。この汚染発病土１００ｇに対し２５ｍｌの蒸留水を散水して加水した後、５０〜１００ｐｐｍの次亜塩素 酸水溶液を２５ｍｌ散布し、１０日間遮光フィルムで覆い、その後１区１６粒播種して４日後の健全発病率を判定した。この試験を５回反復してその結果、平均２１．８％の健全苗率を得た。 Prepare soil with a degree of contamination of 5% using soil contaminated with Komatsuna wilt disease. After watering 100 g of this contaminated soil with 25 ml of distilled water, 25 ml of a 50 to 100 ppm hypochlorous acid aqueous solution was sprayed, covered with a light-shielding film for 10 days, and then 16 seeds in 1 group were sown 4 days later. The healthy incidence of the disease was determined. This test was repeated 5 times, resulting in an average healthy seedling rate of 21.8%.
ｐH６、３０００ｐｐｍの次亜塩素酸を含む水溶液を土壌に散布すると、その強い殺菌力によって土壌中の真菌及び細菌の大部分が死んで菌数が減少し、生き残った一部も増殖する能力に乏しい状態となる。ｐH５〜６．５の１０００〜６０００ｐｐｍ、好ましくは １５００〜４５００ｐｐｍの次亜塩素酸水溶液は、遊離酸としては不安定であって速やかに分解されるから、土壌中に長期間残留せず、７日程度で消失する。ｐH５〜６．５の１０００〜６０００ｐｐｍ、１５００〜４５００ｐｐｍの次亜塩素酸水溶液は、強い殺菌力 を有するが二酸化塩素のような高い反応性も爆発の危険性も有しないから、作業者の健康 を損なう虞もない。また、使用量は混和発病土１００ｇに対し、２５〜５０ｍｌの少量でも１００ｍｌ以上の使用量に匹敵する効果を有するので、残留塩素の問題も生じない。 When an aqueous solution containing hypochlorous acid with a pH of 6 and 3000 ppm is sprayed on the soil, most of the fungi and bacteria in the soil die due to its strong bactericidal activity, the number of bacteria decreases, and the surviving part also lacks the ability to grow. It becomes a state. An aqueous solution of hypochlorous acid having a pH of 5 to 6.5 of 1000 to 6000 ppm, preferably 1500 to 4500 ppm, is unstable as a free acid and is rapidly decomposed, so that it does not remain in the soil for a long period of time for 7 days. It disappears in a degree. Hypochlorous acid aqueous solutions having a pH of 5 to 6.5 of 1000 to 6000 ppm and 1500 to 4500 ppm have strong bactericidal activity, but do not have the high reactivity and the risk of explosion like chlorine dioxide. There is no risk of damage. Further, since the amount used is equivalent to the amount used of 100 ml or more even with a small amount of 25 to 50 ml with respect to 100 g of mixed diseased soil, the problem of residual chlorine does not occur.
１０００〜６０００ｐｐｍ、好ましくは１５００〜４５００ｐｐｍの範囲でいずれの濃度の次亜塩素酸水溶液を用いるかは、汚染度に応じて選択される。汚染度５％の混和発病土では、ｐH６の３０００ｐｐｍの次亜塩素酸水溶液が適切であったが、汚染度によって 、１０００〜６０００ｐｐｍ、好ましくは１５００〜４５００ｐｐｍの範囲で有効濃度が 選択される。また、土壌への加水度によって１０００〜６０００ｐｐｍ、好ましくは１５ ００〜４５００ｐｐｍの範囲で有効濃度が選択される。本発明の育種後は、これによって 、有用な土壌微生物が優勢となり病害菌の増殖が更に抑制されるから、土壌中の微生物の バランスは、植物の栽培に適した状態となる。次亜塩素酸は、この時点では既に分解し消 失しているから、肥料に含まれていた土壌微生物の増殖を妨げない。肥料に含まれる肥効 成分も、植物の栽培に適した環境を生み出すのに資する。 Which concentration of the hypochlorous acid aqueous solution to be used in the range of 1000 to 6000 ppm, preferably 1500 to 4500 ppm is selected according to the degree of contamination. For mixed soil with a degree of contamination of 5%, an aqueous solution of hypochlorous acid having a pH of 3000 ppm was suitable, but an effective concentration is selected in the range of 1000 to 6000 ppm, preferably 1500 to 4500 ppm, depending on the degree of contamination. Further, the effective concentration is selected in the range of 1000 to 6000 ppm, preferably 1500 to 4500 ppm depending on the degree of water addition to the soil. After breeding of the present invention, useful soil microorganisms predominate and the growth of disease-causing bacteria is further suppressed, so that the balance of microorganisms in the soil becomes a state suitable for plant cultivation. Hypochlorous acid has already decomposed and disappeared at this point, so it does not interfere with the growth of soil microorganisms contained in the fertilizer. The fertilizer components contained in fertilizer also contribute to creating an environment suitable for plant cultivation.
上記した実施例ではコマツナ立ち枯れ病汚染土壌への次亜塩素酸水溶液処理の効果を示したが、その他の、例えば、紋羽病，菌核病，うどんこ病，さび病等の病害に対して有効である。
In the above-mentioned examples, the effect of the hypochlorous acid aqueous solution treatment on the soil contaminated with Komatsuna wilt disease was shown, but for other diseases such as crest feather disease, sclerotium disease, powdery mildew, and rust disease. It is valid.
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