JP6862349B2 - 発酵経路を経由するフラックスの増大を示す組み換え微生物体 - Google Patents
発酵経路を経由するフラックスの増大を示す組み換え微生物体 Download PDFInfo
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- JP6862349B2 JP6862349B2 JP2017549170A JP2017549170A JP6862349B2 JP 6862349 B2 JP6862349 B2 JP 6862349B2 JP 2017549170 A JP2017549170 A JP 2017549170A JP 2017549170 A JP2017549170 A JP 2017549170A JP 6862349 B2 JP6862349 B2 JP 6862349B2
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- acetolactic acid
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- butanediol
- enzyme
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Description
本出願は、2014年12月8日に出願された米国特許出願第62/089,053号明細書、及び2015年6月1日に出願された米国特許出願第62/168,969号明細書の利益を主張するものであり、それら全体が、参照により本明細書に援用される。
以下の実施例により本発明がさらに解説されるが、それらはもちろん、決して本発明の範囲を限定するものとはみなされるべきではない。
本発明の様々な実施形態を以下に示す。
1.ピルビン酸:フェレドキシン酸化還元酵素(EC 1.2.7.1)、アセト乳酸合成酵素 (EC 2.2.1.6)、及びアセト乳酸脱炭酸酵素 (EC 4.1.1.5)からなる群から選択される1つ以上の酵素を備えた、カルボキシドトロフィックな組み換えクロストリジウム属細菌であって、各酵素は、過剰発現された内因性酵素、変異した内因性酵素、又は外因性酵素である、前記細菌。
2.ピルビン酸:フェレドキシン酸化還元酵素、及びアセト乳酸合成酵素を備える、上記1に記載の細菌。
3.ピルビン酸:フェレドキシン酸化還元酵素、及びアセト乳酸脱炭酸酵素を備える上記1に記載の細菌。
4.アセト乳酸合成酵素、及びアセト乳酸脱炭酸酵素を備える上記1に記載の細菌。
5.ピルビン酸:フェレドキシン酸化還元酵素、アセト乳酸合成酵素、及びアセト乳酸脱炭酸酵素を備える上記1に記載の細菌。
6.前記酵素が、過剰発現された内因性ピルビン酸:フェレドキシン酸化還元酵素である、上記1に記載の細菌。
7.前記酵素が、外因性デスルホビブリオ・アフリカヌスのピルビン酸:フェレドキシン酸化還元酵素である、上記1に記載の細菌。
8.前記酵素が、過剰発現された内因性のIlvB ORF2059アセト乳酸合成酵素、過剰発現された内因性のIlvB ORF2336アセト乳酸合成酵素、過剰発現された内因性のIlvNアセト乳酸合成酵素、又は過剰発現された内因性のAlsSアセト乳酸合成酵素である、上記1に記載の細菌。
9.前記酵素が、変異した内因性IlvNアセト乳酸合成酵素である、上記1に記載の細菌。
10.前記酵素が、外因性の枯草菌アセト乳酸合成酵素である、上記1に記載の細菌。
11.前記酵素が、過剰発現された内因性AlsDアセト乳酸脱炭酸酵素、又は過剰発現された内因性BudAアセト乳酸脱炭酸酵素である、上記1に記載の細菌。
12.前記酵素が、外因性アエロモナス ハイドロフィラのアセト乳酸脱炭酸酵素である、上記1に記載の細菌。
13.前記酵素が、外因性ロイコノスティック ラクティスのアセト乳酸脱炭酸酵素である、上記1に記載の細菌。
14.前記細菌が、クロストリジウム オートエタノゲナム、クロストリジウム リュングダリィ、又はクロストリジウム ラグスダレイから誘導される、上記1に記載の細菌。
15.前記細菌が、クロストリジウム オートエタノゲナムから誘導される、上記1に記載の細菌。
16.前記細菌が、DSMZ アクセッション番号DSM23693で寄託されているクロストリジウム オートエタノゲナムから誘導される、上記1に記載の細菌。
17.発酵産物を作製するためにCOを含有するガス状基質の存在下で、上記1に記載の細菌を発酵させることを含む、発酵産物を作製する方法。
18.前記発酵産物が、エタノール、ブタノール、イソプロパノール、イソブタノール、高級アルコール、ブタンジオール、2,3−ブタンジオール、コハク酸塩、イソプレノイド、脂肪酸、バイオポリマー、及びそれらの混合物からなる群から選択される、上記17に記載の方法。
19.前記発酵産物が、2,3−ブタンジオールである、上記18に記載の方法。
Claims (16)
- ピルビン酸:フェレドキシン酸化還元酵素(EC 1.2.7.1)、アセト乳酸合成酵素 (EC 2.2.1.6)、及びアセト乳酸脱炭酸酵素 (EC 4.1.1.5)を備える組み換え細菌であって、各酵素は、過剰発現された内因性酵素又は外因性酵素であり、前記細菌はクロストリジウム オートエタノゲナム、クロストリジウム リュングダリィ、又はクロストリジウム ラグスダレイから誘導され、かつ2,3−ブタンジオール及び酢酸を産生する、前記細菌。
- 前記ピルビン酸:フェレドキシン酸化還元酵素が、過剰発現された内因性ピルビン酸:フェレドキシン酸化還元酵素である、請求項1に記載の細菌。
- 前記ピルビン酸:フェレドキシン酸化還元酵素が、デスルホビブリオ・アフリカヌスのピルビン酸:フェレドキシン酸化還元酵素である、請求項1に記載の細菌。
- 前記アセト乳酸合成酵素が、過剰発現された内因性のIlvB アセト乳酸合成酵素、過剰発現された内因性のIlvNアセト乳酸合成酵素、又は過剰発現された内因性のAlsSアセト乳酸合成酵素である、請求項1に記載の細菌。
- 前記アセト乳酸合成酵素が、フィードバック不感受性のIlvNアセト乳酸合成酵素である、請求項1に記載の細菌。
- 前記アセト乳酸合成酵素が、外因性の枯草菌アセト乳酸合成酵素である、請求項1に記載の細菌。
- 前記アセト乳酸脱炭酸酵素が、過剰発現された内因性AlsDアセト乳酸脱炭酸酵素、又は過剰発現された内因性BudAアセト乳酸脱炭酸酵素である、請求項1に記載の細菌。
- 前記アセト乳酸脱炭酸酵素が、外因性アエロモナス ハイドロフィラのアセト乳酸脱炭酸酵素である、請求項1に記載の細菌。
- 前記アセト乳酸脱炭酸酵素が、外因性ロイコノスティック ラクティスのアセト乳酸脱炭酸酵素である、請求項1に記載の細菌。
- 前記細菌が、クロストリジウム オートエタノゲナムから誘導される、請求項1に記載の細菌。
- 前記細菌が、DSMZ アクセッション番号DSM23693で寄託されているクロストリジウム オートエタノゲナムから誘導される、請求項1に記載の細菌。
- 過剰発現された内因性又は外因性2,3−ブタンジオール脱水素酵素(EC 1.1.1.4)を含まない、請求項1に記載の細菌。
- 過剰発現された内因性又は外因性ピルビン酸:フェレドキシン酸化還元酵素、アセト乳酸合成酵素、及びアセト乳酸脱炭酸酵素を備えない細菌よりも、高い量の2,3−ブタンジオールを産生する、請求項1に記載の細菌。
- 57mmol/L/日よりも速い速度で2,3−ブタンジオールを産生する、請求項1に記載の細菌。
- 122mmol/L/dayよりも速い速度で2,3−ブタンジオールを産生する、請求項1に記載の細菌。
- 2,3−ブタンジオールを作製するためにCOを含有するガス状基質の存在下で、請求項1に記載の細菌を発酵させることを含む、2,3−ブタンジオールを作製する方法。
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AU2015360786B2 (en) | 2021-10-21 |
PL3230459T3 (pl) | 2021-03-08 |
KR102493197B1 (ko) | 2023-01-30 |
WO2016094334A1 (en) | 2016-06-16 |
EP3230459B1 (en) | 2020-09-16 |
CA2969233A1 (en) | 2016-06-16 |
DK3230459T3 (da) | 2020-12-07 |
JP7139478B2 (ja) | 2022-09-20 |
CN107002028A (zh) | 2017-08-01 |
CA2969233C (en) | 2020-06-23 |
JP2021104040A (ja) | 2021-07-26 |
US20160160223A1 (en) | 2016-06-09 |
EP3230459A4 (en) | 2018-06-20 |
KR20170121147A (ko) | 2017-11-01 |
PT3230459T (pt) | 2020-12-18 |
EP3230459A1 (en) | 2017-10-18 |
EA201791197A1 (ru) | 2017-11-30 |
HUE052810T2 (hu) | 2021-05-28 |
ES2836800T3 (es) | 2021-06-28 |
JP2017536854A (ja) | 2017-12-14 |
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