JP6856618B2 - 接着性ペプチド - Google Patents
接着性ペプチド Download PDFInfo
- Publication number
- JP6856618B2 JP6856618B2 JP2018501837A JP2018501837A JP6856618B2 JP 6856618 B2 JP6856618 B2 JP 6856618B2 JP 2018501837 A JP2018501837 A JP 2018501837A JP 2018501837 A JP2018501837 A JP 2018501837A JP 6856618 B2 JP6856618 B2 JP 6856618B2
- Authority
- JP
- Japan
- Prior art keywords
- present application
- peptide
- seq
- cells
- adhesion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/18—Growth factors; Growth regulators
- A61K38/1875—Bone morphogenic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/10—Polypeptides; Proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/08—Peptides having 5 to 11 amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/10—Peptides having 12 to 20 amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
- A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
- C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
- C09J189/00—Adhesives based on proteins; Adhesives based on derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0619—Neurons
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/06—Flowable or injectable implant compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2539/00—Supports and/or coatings for cell culture characterised by properties
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Surgery (AREA)
- Neurology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
Description
[X1−X2−X3−X4−X5]n
前記X1は任意のアミノ酸で、
X2、X3及びX4は、L、V、I、E及びAのうちいずれか一つのアミノ酸であり、各々同じまたは異なり、X5は、KまたはRのアミノ酸である。
[X6−X7−X8−X9−X10−X11]n
X6は、F、Y及びWのうちいずれか一つのアミノ酸で、
X7は、KまたはRのアミノ酸で、
X8は、A、M及びIのうちいずれか一つのアミノ酸で、
X9は、L、M及びGのうちいずれか一つのアミノ酸で、
X10は、任意のアミノ酸で、
X11は、C、S及びTのうちいずれか一つのアミノ酸である。
前記化学式Iにおいて、前記X2、X3またはX4のうち一つ以上は欠如されてもよい。
X12 1−15
[X1−X2−X3−X4−X5]n
X1は、極性の非荷電された任意のアミノ酸であり、
X2、X3及びX4は、L、V、I、E及びAのうちいずれか一つのアミノ酸であり、各々同じまたは異なり、
X5は、KまたはRのアミノ酸である。
[X6−X7−X8−X9−X10−X11]n
X6は、F、Y及びWのうちいずれか一つのアミノ酸で、
X7は、KまたはRのアミノ酸で、
X8は、A、M及びIのうちいずれか一つのアミノ酸で、
X9は、L、M及びGのうちいずれか一つのアミノ酸で、
X10は、任意のアミノ酸で、
X11は、C、S及びTのうちいずれか一つのアミノ酸である。
前記nは1または2である。
<実施例1.接着性ペプチドの製造>
細胞接着には、ペプチドを予めコートする方法とコートせず細胞培養液に直接添加して培養する二種類の方法で観察し、一致する結果を示した。コート方法を取る場合、PBSまたは、ウシ胎児血清を含まない細胞培養培地に一定濃度のペプチドを添加して、常温、30分間コートした後、溶液を除去した後、細胞を添加して多様な時間にかけて接着を測定した。また、培養培地にペプチドを添加する場合、細胞を懸濁する時、一定水準のペプチドを共に添加してよく懸濁した後培養皿に移した。細胞接着測定は次の通りに行われた。細胞接着は、ペプチドを添加しなかった場合を陰性対照群に、PBSそしてpoly−L−lysinをコートした場合を陽性対照群にして光学顕微鏡を介して通常の観察、アクチンフィラメント構造及び粗い細胞膜境界形態観察のために、F−actin染色及び共焦点顕微鏡分析、そして接着に対する定量化のために細胞の消費量またはDNA量を測定した。消費量は、CCK−8(Dojindo)を利用して、吸光度を測定して、DNA量は、ピコグリーン定量キット(Picogreenassay kit(Life Technologies))を製造者の方法のとおり利用して蛍光値を測定した。接着された細胞だけを定量化するために、接着されなかった細胞は捨ててPBSでさらに2回洗浄して表面に付いている細胞だけを消費量またはDNAを定量に使用した。
まず、タンパク質合成を阻害すると知られているEDTA、シクロヘキサミド(CHX)(10μM、37℃1時間)またはGAGで細胞を処理した後上述したような接着能を実験した。
次は、非タンパク質物質の関与の有無を調べて、その対象に細胞表面の細胞外メトリックス(Extracellualrmatrix,ECM)に多量含まれているプロテオグリカンとの関連性を分析した。
プロテオグリカンを構成する主な成分であるヘパラン硫酸(heparan sulfate)、ヘパリン(heparin)、コンドロイチン硫酸(chondroitin sulfate)、デルマタン硫酸(dermatansulfate)、ケラタン硫酸(keratan sulafate)等のグリコアミノグリカン(glycoaminoglycan)(GAG)は、ECMを構成する主な成分でもあるが、ECM構造の保存性(structuralintegrity of ECM)を維持して細胞の形態を維持すると共に、細胞の接着、細胞の極性(cellpolarity)を調節する。ECMが有するこのような機能は、環境に対する細胞の適応を誘導すると共に複雑な一連の代謝過程と直接的に関連していて、様々な生理学的役割を調節する複合的機能を有する。プロテオグリカンに対する本願ペプチドの分子親和力は、(i)アフィニティークロマトグラフィー、(ii)精製されたGAG成分を利用した競争的結合を介した細胞接着調査、(iii)GAG成分を含むECMを特異的に分解する酵素を処理してペプチドによる細胞接着促進の抑制調査、以上の三つの方法を介して検証した。酵素処理の場合、ヒアルロニダーゼ(hyaluronidase(Sigma))、コラゲナーゼ(collagenase(Sigma))を処理した。精製されたGAGは、hyaluronan(Sigma)、コンドロイチン硫酸(chondroitinsulfate(Sigma))、ヘパリン(heparin(Sigma))、ヘパラン硫酸(heparan sulfate(Sigma))を各々使用した。
(3)RGDと比較
Claims (10)
- 配列番号16〜22、24〜27、29〜37、42〜64、67及び68からなる群から選択されるアミノ酸配列からなる単離されたポリペプチド。
- 配列番号16〜20、22、24〜27、30〜37、42〜64、67及び68の第1アミノ酸はリシン残基に置換され、配列番号21の第1アミノ酸はアルギニンに置換され、配列番号29の第1アミノ酸はアスパラギン酸に置換されている請求項1に記載のポリペプチド。
- 配列番号16〜22、24〜27、30〜37、42〜64、67及び68の第1アミノ酸はアスパラギン酸又はグルタミン酸残基に置換され、配列番号29の第1アミノ酸はリシン又はアルギニン残基に置換されている請求項1に記載のポリペプチド。
- 配列番号16〜20、22、24〜27、30〜37、42〜64、67又は68のアミノ酸配列はN末端に14個以下のアルギニン残基をさらに含み、配列番号21のアミノ酸配列はN末端に14個以下のリシン残基をさらに含み、配列番号29のアミノ酸配列は14個以下のグルタミン酸残基をさらに含む請求項1に記載のポリペプチド。
- 前記ポリペプチドのN又はC−末端は、非反応性基で置き換えられたものである、請求項1乃至4のいずれか一項に記載のポリペプチド。
- 請求項1乃至4のいずれか一項に記載のポリペプチドをコードする、核酸分子。
- 請求項6に記載の核酸分子を含み、請求項1乃至4のいずれか一項に記載のポリペプチドを発現する、ベクター。
- イン・ビトロにおける、物質の接着性の向上のための請求項1乃至4のいずれか一項に記載のポリペプチドの使用。
- 請求項1乃至4のいずれか一項に記載のポリペプチドを含む接着用組成物。
- イン・ビトロにおいて、第1の生物学的若しくは非生物学的物質、及び/又は第2の生物学的若しくは非生物学的物質に請求項1に記載のポリペプチドを処理することにより、前記第1の生物学的若しくは非生物学的物質を第2の生物学的若しくは非生物学的物質に付着させる方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020150042167A KR101794401B1 (ko) | 2015-03-26 | 2015-03-26 | 접착성 펩타이드 및 그 용도 |
KR10-2015-0042167 | 2015-03-26 | ||
PCT/KR2016/002864 WO2016153256A1 (ko) | 2015-03-26 | 2016-03-22 | 접착성 펩타이드 및 그 용도 |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2018511344A JP2018511344A (ja) | 2018-04-26 |
JP2018511344A5 JP2018511344A5 (ja) | 2019-04-25 |
JP6856618B2 true JP6856618B2 (ja) | 2021-04-07 |
Family
ID=56977649
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2018501837A Active JP6856618B2 (ja) | 2015-03-26 | 2016-03-22 | 接着性ペプチド |
Country Status (6)
Country | Link |
---|---|
US (1) | US10857258B2 (ja) |
EP (1) | EP3279209A4 (ja) |
JP (1) | JP6856618B2 (ja) |
KR (1) | KR101794401B1 (ja) |
CN (1) | CN107922459B (ja) |
WO (1) | WO2016153256A1 (ja) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9801972B2 (en) | 2014-10-02 | 2017-10-31 | Postech Academy-Industry Foundation | Mussel-inspired bioactive surface coating composition generating silica nanoparticles |
KR20160040063A (ko) * | 2014-10-02 | 2016-04-12 | 포항공과대학교 산학협력단 | 생활성 융합단백질 및 이를 포함하는 실리카 나노입자를 포함하는 표면 코팅제 |
KR101865451B1 (ko) * | 2015-08-26 | 2018-07-13 | 윤원준 | 무기물 표면 접착 또는 코팅용 단백질 접착제 |
US20230181677A1 (en) * | 2016-08-26 | 2023-06-15 | Sewon Biotechnology Inc. | Polypeptide for promoting bone regeneration or bone formation and its use |
WO2018048290A1 (ko) * | 2016-09-12 | 2018-03-15 | 윤원준 | 피더세포 없이 줄기세포 배양이 가능한 배양 조성물 및 방법 |
WO2018052245A1 (ko) * | 2016-09-13 | 2018-03-22 | 윤원준 | 세포접착용 조성물 및 방법 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0625290A (ja) * | 1992-07-10 | 1994-02-01 | Sumitomo Pharmaceut Co Ltd | 細胞接着活性ペプチド |
WO1999066964A1 (en) | 1998-06-23 | 1999-12-29 | Surgical Sealants, Incorporated | Carbodiimide cross-linked albumin for bioadhesives, surgical sealants and implantable devices |
US20020022588A1 (en) | 1998-06-23 | 2002-02-21 | James Wilkie | Methods and compositions for sealing tissue leaks |
KR100885071B1 (ko) * | 2002-05-14 | 2009-02-25 | 재단법인서울대학교산학협력재단 | 세포에 부착하는 올리고펩타이드 |
JP3906924B2 (ja) * | 2003-02-28 | 2007-04-18 | 独立行政法人農業生物資源研究所 | 絹タンパクから細胞生育ペプチドの抽出と利用 |
JPWO2005068495A1 (ja) * | 2004-01-13 | 2008-01-10 | 東レ株式会社 | クモ糸タンパク質を含む絹糸および該絹糸を産生するカイコ |
AU2006216635B2 (en) * | 2005-02-25 | 2011-11-17 | University Of Utah Research Foundation | Tropoelastin for promoting endothelial cell adhesion or migration |
JP2007252327A (ja) * | 2006-03-24 | 2007-10-04 | Toray Ind Inc | 細胞接着性絹糸及びその製造方法 |
KR101094445B1 (ko) * | 2009-05-13 | 2011-12-15 | 한국과학기술연구원 | 줄기세포에 대한 접착활성을 갖는 섬유아세포 성장인자의 재조합 단백질 및 이를 이용한 줄기세포의 배양방법 |
KR101209233B1 (ko) * | 2011-02-21 | 2012-12-06 | 서울대학교산학협력단 | 피브린 결합능을 가진 생리활성 펩타이드가 함유된 피브린 수화젤 |
KR101865451B1 (ko) * | 2015-08-26 | 2018-07-13 | 윤원준 | 무기물 표면 접착 또는 코팅용 단백질 접착제 |
-
2015
- 2015-03-26 KR KR1020150042167A patent/KR101794401B1/ko active IP Right Grant
-
2016
- 2016-03-22 WO PCT/KR2016/002864 patent/WO2016153256A1/ko active Application Filing
- 2016-03-22 JP JP2018501837A patent/JP6856618B2/ja active Active
- 2016-03-22 CN CN201680028082.8A patent/CN107922459B/zh active Active
- 2016-03-22 EP EP16769074.2A patent/EP3279209A4/en not_active Withdrawn
-
2017
- 2017-09-25 US US15/714,357 patent/US10857258B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
JP2018511344A (ja) | 2018-04-26 |
US10857258B2 (en) | 2020-12-08 |
US20180085488A1 (en) | 2018-03-29 |
CN107922459A (zh) | 2018-04-17 |
KR101794401B1 (ko) | 2017-11-06 |
EP3279209A1 (en) | 2018-02-07 |
CN107922459B (zh) | 2021-06-25 |
EP3279209A4 (en) | 2019-01-02 |
KR20160115115A (ko) | 2016-10-06 |
WO2016153256A1 (ko) | 2016-09-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP6856618B2 (ja) | 接着性ペプチド | |
JP6430595B2 (ja) | 修飾自己組織化ペプチド | |
EP1636250B1 (en) | Self-assembling peptides incorporating modifications and uses thereof | |
US7713923B2 (en) | Self-assembling peptides incorporating modifications and methods of use thereof | |
Alshehri et al. | Scaffolds from self-assembling tetrapeptides support 3D spreading, osteogenic differentiation, and angiogenesis of mesenchymal stem cells | |
Xu et al. | A carboxymethyl chitosan and peptide-decorated polyetheretherketone ternary biocomposite with enhanced antibacterial activity and osseointegration as orthopedic/dental implants | |
Yaylaci et al. | Chondrogenic differentiation of mesenchymal stem cells on glycosaminoglycan-mimetic peptide nanofibers | |
Zhang et al. | Compatibility of neural stem cells with functionalized self-assembling peptide scaffold in vitro | |
US10851132B2 (en) | Protein based adhesive composition and method | |
Revkova et al. | Spidroin silk fibers with bioactive motifs of extracellular proteins for neural tissue engineering | |
Scott et al. | Three-dimensional cell entrapment as a function of the weight percent of peptide-amphiphile hydrogels | |
Wang et al. | Spatial micro-variation of 3D hydrogel stiffness regulates the biomechanical properties of hMSCs | |
Zhang et al. | Biomimetic cell-adhesive ligand-functionalized peptide composite hydrogels maintain stemness of human amniotic mesenchymal stem cells | |
JP2021514676A (ja) | 幹細胞の拡大および分化 | |
KR101794482B1 (ko) | 생체 또는 비생체 접착성 펩타이드 및 그 용도 | |
KR102010871B1 (ko) | 세포접착용 조성물 및 방법 | |
JP6797389B1 (ja) | 細胞外マトリックス含有組成物及びその製造方法、並びに三次元組織体及びその製造方法 | |
Bai et al. | A Novel Method to Improve the Physical Property and Biocompatibility of Decellularized Heart Valve Scaffold with Sericin and Polydopamine | |
WO2023187065A1 (en) | Coated article for culturing primary cells and stem cells and method for preparing the same | |
JPWO2019163948A1 (ja) | 細胞培養用基材及び培養方法 | |
Perugini | Poly-ɛ-lysine Dendron Aptamers as Regulators of Angiogenesis in Tissue Regeneration | |
Ceylan | Design, Synthesis and Characterization of Bioinspired Nanomaterials for Engineering and Biomedicine | |
Ahmed et al. | cell so |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A711 | Notification of change in applicant |
Free format text: JAPANESE INTERMEDIATE CODE: A711 Effective date: 20180725 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20180725 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20180820 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20190315 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20190315 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20200324 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20200623 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20201117 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20210210 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20210302 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20210318 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 6856618 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |