JP6832848B2 - 組織試料処理システム及びそれに関連する方法 - Google Patents
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Description
本出願は、2014年10月20日に出願された米国仮出願第62/066,232号の利益を主張するものであり、これは、参照により本明細書に組み込まれる。
本発明の記載及び特許請求において、以下の用語を使用する。
図1は、本開示の1つの例による組織試料処理システム100を、模式的に図示している。組織試料処理システム100は、組織試料102と、必要に応じてキャリア流体104とを受容する、微小流体分離システム110を含み得る。以下に詳しく説明するように、微小流体分離システム110内での、組織試料102とキャリア流体104との流れは、組織試料102内の材料を、そのサイズに基づいて、複数のサイズ画分に分離するのを容易にする。したがって、微小流体分離システム110は、組織試料内の(サイズに基づく)ある具体的なタイプの細胞を、他の細胞から分離するように構成され得る。1つの例では、組織試料102は、***細胞(例えば、運動している***細胞及び/又は運動していない***細胞)を含む、***又は***試料を含み得る。一部のケースでは、組織試料又は試料流体は、追加された別のキャリア流体を有しない、(すなわち、キャリア流体は、自然の***である)流体の***試料であり得る。あるいは、所望の流体の流れを容易にするために、追加的なキャリア流体が添加され得る。微小流体分離システム110は、***細胞を、組織試料内の他の細胞から分離するように、例えば、運動していない***細胞を、赤血球(RBC)から分離するように構成され得る。1つの態様では、微小流体分離システム110は、例えば、***を生成するために潜在的に使用され得る精原幹細胞又は他の***前駆体のような、数種類の細胞型を仕分けし得る。組織試料処理システム100は、***細胞を、体細胞組織細胞よりも濃縮するために用いられ得る。微小流体分離システム110からの出力は、その出力中の所望の細胞タイプの解像度を向上させるために、再処理106され得る。例えば、以下に説明するような、任意の好適なタイプの微小流体分離システム110が使用可能である。
Claims (16)
- ***細胞を分離する方法であって、
取り入れ口ゾーンに設けられた少なくとも1つの取り入れ口と、前記取り入れ口ゾーンとは反対側の排出口ゾーンに設けられた複数の排出口とを備えた流体チャネルを有し、前記流体チャネルがらせん状形状を備える、微小流体分離システムを入手すること、及び
***試料を前記流体チャネル内に配置すること、を含み、前記流体チャネル内での前記***試料の流れは、前記***試料内の材料を、サイズと形状に基づいて、複数のサイズ形状画分に分離するのを容易にし、前記複数の排出口のそれぞれが、前記***試料内の前記材料の異なるサイズ形状画分を受容するようになっている、方法。 - 前記取り入れ口ゾーンが、キャリア流体を受容するためのキャリア流体取り入れ口と、前記組織試料を受容するための組織試料取り入れ口とを有し、
前記微小流体分離システムが更に、前記取り入れ口ゾーンと前記排出口ゾーンとの間にあって前記キャリア流体及び前記組織試料に対して開かれている移送領域を備え、
前記移送領域内の交差流が、前記組織試料内の前記材料を、サイズと形状に基づいて分離するのを容易にする、請求項1に記載の方法。 - 前記複数の排出口のうちの少なくとも1つに関連付けられる、前記***試料内の前記材料は、第1のアリコートを成す運動していない***細胞を含む、請求項1に記載の方法。
- 仕分けシステムを、前記複数の排出口のうちの少なくとも1つと関連付けることを更に含み、前記複数の排出口のうちの前記少なくとも1つと関連付けられる前記***試料内の前記材料は、運動していない***細胞を含み、かつ、
前記運動していない***細胞を、複数のアリコートに仕分けることを更に含む、請求項1に記載の方法。 - 前記仕分けシステムが、
前記複数の排出口のうちの前記少なくとも1つに関連付けられる前記組織試料内の前記材料を受容するための取り入れ口と、
複数のアリコート保管チャネルと、
前記複数のアリコート保管チャネルと関連付けられ、前記複数のアリコートを前記アリコート保管チャネルに分離するのを容易にする、複数のバルブと、を備える、請求項4に記載の方法。 - 前記複数の排出口のうちの第2の排出口に関連付けられる、前記***試料内の前記材料は、第2のアリコートを成す運動している***細胞を含む、請求項3に記載の方法。
- 前記複数の排出口のうちの第3の排出口に関連付けられる、前記***試料内の前記材料は、第3のアリコートを成す赤血球を含む、請求項3に記載の方法。
- 前記第1のアリコートに含まれる赤血球を低減するために、赤血球抗体を前記流体チャネルに導入することを更に含む、請求項7記載の方法。
- 前記複数の排出口のうちの第4の排出口に関連付けられる、前記***試料内の前記材料は、精原幹細胞及び***前駆体細胞のうちの少なくとも1つを含む、請求項1に記載の方法。
- 前記複数の排出口は、異常な倍数体の***細胞及び正常な倍数体の***細胞のうちの少なくとも1つの分離された材料を含む、請求項1に記載の方法。
- 試薬が添加されることなく前記方法が実施される、請求項1に記載の方法。
- 前記複数の排出口からの少なくとも1つのアリコートを冷凍保存して当該アリコートに含まれる試料を冷凍することを更に含む、請求項1に記載の方法。
- 前記流体チャネル内の交差流が内側の流体流れ層内の運動していない***細胞を分離するのを容易にし、前記複数の排出口のうちの1つが運動していない***細胞を含む、請求項2に記載の方法。
- 前記運動していない***細胞を、複数のアリコートに仕分けることを更に含み、前記複数のアリコートのそれぞれは、約1〜約20個の運動していない***細胞を含む、請求項13に記載の方法。
- 前記複数のアリコートのそれぞれは単一の運動していない***細胞を含む、請求項14に記載の方法。
- 前記らせん状形状は内側の流れ層及び外側の流れ層を含み、前記内側の流れ層内において運動していない***細胞が集められて前記複数の排出口のうちの1つを介して排出される、請求項1記載の方法。
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2015
- 2015-10-20 WO PCT/US2015/056494 patent/WO2016064896A1/en active Application Filing
- 2015-10-20 EP EP15852164.1A patent/EP3209766A4/en not_active Withdrawn
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EP3209766A1 (en) | 2017-08-30 |
CA2965138A1 (en) | 2016-04-28 |
EP3209766A4 (en) | 2018-08-15 |
US11708556B2 (en) | 2023-07-25 |
WO2016064896A1 (en) | 2016-04-28 |
JP2017531440A (ja) | 2017-10-26 |
US20170306288A1 (en) | 2017-10-26 |
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