JP6796932B2 - 分離装置 - Google Patents
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Description
本実施形態の説明の前に、誘電泳動の概要について説明する。細菌や細胞等の誘電体粒子を含む試料液に対して電極を配置し、電極に周波数ωの交流電圧を供給した場合、試料液中の誘電体粒子に誘電泳動力が作用する。この誘電泳動力FDEPは、次式で表される。
FDEP=2πr3εmRe[K(ω)]∇E2 …(1)
K(ω)=(εp *−εm *)/(εp *+2εm *) …(2)
以下、実施形態1に係る分離装置を図1〜5を用いて説明する。
1−1.分離装置
図1は、実施形態1に係る分離装置の構成を示す図である。図1に示す分離装置1は、目的細胞及びそれ以外の細胞を含む懸濁液において、誘電泳動を利用して目的細胞を分離する。分離装置1は、液導入部10と、流路20と、電極部30と、電源部40と、制御部50と、回収部60とを備える。
図2は、実施形態1に係る分離装置1の電極部30の構成を示す斜視図であり、図3は、実施形態1に係る分離装置1の電極部30の構成を示す平面図である。電極部30は、複数のシグナル電極31と、複数のグランド電極32と、シグナル配線35と、グランド配線36とを備える。
以上のように構成される分離装置1について、その動作を図4及び図5を参照して以下に説明する。
以上説明したように、本実施形態によれば、電極部30の電極31、32が、細胞(誘電体粒子)が流れる流路20の高さ方向に延びる立体形状(3次元形状)をなすので、流路20の高さを高くしても、流路20の下方を通る細胞から流路20の上方を通る細胞まで均一な誘電泳動力を作用させることができる。そのため、細胞の分離処理有効体積を大きくすることができ、分離処理能力を向上することができる。
本実施形態1では、電極31、32を通過する目的細胞Aに正の誘電泳動力(引力)を作用させ、目的細胞Aを電極31、32に吸着させて捕捉した。しかし、本開示の思想はこれに限らず、電極31、32を通過する目的細胞Aに負の誘電泳動力(斥力)を作用させ、電極31、32に反発させて目的細胞Aを電極31、32間に捕捉してもよい。
実施形態1では、目的細胞に強い正の誘電泳動力(引力)を作用させて目的細胞を電極31、32に捕捉し、目的細胞以外の細胞を破棄し、その後、電極31、32間への交流電圧の印加を停止して、電極31、32に捕捉された目的細胞を回収して分離した。実施形態2では、目的細胞に比較的に弱い正の誘電泳動力(引力)を作用させて、目的細胞とそれ以外の細胞との誘電泳動力の強さが異なることにより、目的細胞とそれ以外の細胞とが電極31、32の間を通過する時間が異なることを利用して、目的細胞を分離する(誘電泳動クロマトグラフィー)。
本実施形態では、細胞に作用する誘電泳動力の強さを調節するために、電極31、32間に印加する交流電圧の大きさを調整した。本開示はこれに限定されず、例えば電極31、32への交流電圧の供給及び供給停止を繰り返すことにより、電極31、32への交流電圧の印加時間を調整して、細胞に作用する誘電泳動力の強さを調節してもよい。
実施形態1では、電極部30のシグナル電極31及びグランド電極32を、流路20の液流方向及び幅方向にマトリクス状に配列した。実施形態3では、電極部30のシグナル電極31及びグランド電極32を、流路20の液流方向に対して所定の角度をなす斜め方向に略直線状に配列する。
本実施形態では、シグナル電極31及びグランド電極32を、流路20の幅方向の中央部から両側部に向けて略V字状に配列した。本開示はこれに限定されず、流路20の幅方向の両側部から中央部に向けて略逆V字状に配列してもよい。この場合、目的細胞を含む懸濁液を、流路20の幅方向の両側部に導入すればよい。
実施形態3では、電極部30のシグナル電極31及びグランド電極32を、略等間隔に配列した。実施形態4では、電極部30のシグナル電極31及びグランド電極32を、流路20の中央部から側部に近くなるにつれて電極間隔が次第に広くなるように配列する。
本実施形態でも、シグナル電極31及びグランド電極32を、流路20の幅方向の両側部から中央部に向けて略逆V字状に配列してもよい。この場合、シグナル電極31及びグランド電極32は、電極間隔が側部から中央部に向けて次第に広くなるように配列されればよい。
上記した実施形態1〜4において、本装置の分離対象として細菌や細胞を例示した。本装置の分離対象は、細菌や細胞に限らず、誘電体粒子であればよく、例えば微生物や、真菌、芽胞、ウイルス、DNA、RNA、カーボンナノチューブ、エマルション、マイクロカプセルであってもよい。
10 液導入部
20 流路
30 電極部
31 シグナル電極
32 グランド電極
35 シグナル配線
36 グランド配線
40 電源部
50 制御部
60 回収部
Claims (5)
- 誘電体粒子の分離を行う分離装置であって、
前記誘電体粒子を含む懸濁液を送液する流路と、
前記流路に配置され、前記流路の高さ方向に延びる立体形状の複数の電極と、
前記誘電体粒子が誘電泳動を起こすように、所定周波数の交流電圧を前記複数の電極に印加する電源部と、
前記電源部を制御する制御部と、
前記流路の幅方向の中央部に、前記誘電体粒子を含む懸濁液を導入し、前記流路の幅方向の中央部以外に誘電泳動液を導入する液導入部とを備え、
前記複数の電極は、液流方向に対して所定の角度をなす斜め方向を有し、前記液流方向の上流側に凸となるV字状に配列される、
分離装置。 - 前記複数の電極の電極間隔は、配列方向において次第に広くなり、
前記配列方向における前記電極間隔の変化量は一定である
請求項1に記載の分離装置。 - 前記複数の電極は、前記交流電圧が印加されるシグナル電極と、グランドに接続されるグランド電極とを含み、
前記シグナル電極と前記グランド電極とは、配列方向に隣接する電極が互いに異なるように、交互に配置される、
請求項1又は2に記載の分離装置。 - 前記制御部は、前記複数の電極に捕捉された誘電体粒子を前記流路から送出するための溶出液を前記流路に送液するときに、前記電源部に前記複数の電極への交流電圧の印加を停止させる、
請求項1〜3のいずれか1項に記載の分離装置。 - 前記制御部は、前記誘電体粒子に作用する誘電泳動力の大きさを調整するように、前記複数の電極に印加する交流電圧の大きさ又は印加時間を調整する、
請求項1〜3のいずれか1項に記載の分離装置。
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JP2016026673A JP6796932B2 (ja) | 2016-02-16 | 2016-02-16 | 分離装置 |
US15/999,452 US20210205822A1 (en) | 2016-02-16 | 2017-01-31 | Separation device |
CN201780011565.1A CN108699506A (zh) | 2016-02-16 | 2017-01-31 | 分离装置 |
PCT/JP2017/003301 WO2017141685A1 (ja) | 2016-02-16 | 2017-01-31 | 分離装置 |
EP17752948.4A EP3418373B1 (en) | 2016-02-16 | 2017-01-31 | Separation device |
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JP2016026673A JP6796932B2 (ja) | 2016-02-16 | 2016-02-16 | 分離装置 |
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JP2017143763A5 JP2017143763A5 (ja) | 2019-03-22 |
JP6796932B2 true JP6796932B2 (ja) | 2020-12-09 |
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US (1) | US20210205822A1 (ja) |
EP (1) | EP3418373B1 (ja) |
JP (1) | JP6796932B2 (ja) |
CN (1) | CN108699506A (ja) |
WO (1) | WO2017141685A1 (ja) |
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CN110628568B (zh) * | 2019-09-30 | 2021-10-01 | 北京化工大学 | 用于高通量连续流细胞分离的滑轨式介电泳电极结构 |
US11454583B2 (en) * | 2019-12-27 | 2022-09-27 | Imec Vzw | Field-array free flow fractionation |
CN113717846B (zh) * | 2020-05-26 | 2023-08-29 | 中国科学院青岛生物能源与过程研究所 | 一种基于介电确定性位移的细胞分选芯片、装置及方法 |
EP3919171A1 (en) * | 2020-06-05 | 2021-12-08 | Ecole Polytechnique Fédérale de Lausanne (EPFL) | Dielectrophoresis detection device |
EP4201526A1 (en) * | 2021-12-22 | 2023-06-28 | Imec VZW | Microfluidic device for sorting particles |
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US6071394A (en) * | 1996-09-06 | 2000-06-06 | Nanogen, Inc. | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
WO2000000292A1 (de) * | 1998-06-26 | 2000-01-06 | Evotec Biosystems Ag | Elektrodenanordnung zur dielektrophoretischen partikelablenkung |
GB9916850D0 (en) * | 1999-07-20 | 1999-09-22 | Univ Wales Bangor | Dielectrophoretic apparatus & method |
KR100745754B1 (ko) * | 2005-12-29 | 2007-08-02 | 삼성전자주식회사 | 금속 기둥 전극 구조를 포함하는 유전 영동을 이용하여입자를 조작하기 위한 장치 및 그를 이용하여 빠른유속으로 유전 영동에 의하여 입자를 조작할 수 있는 방법 |
US8702945B2 (en) * | 2007-05-18 | 2014-04-22 | University Of Washington | Time-varying flows for microfluidic particle separation |
US10024819B2 (en) * | 2010-10-21 | 2018-07-17 | The Regents Of The University Of California | Microfluidics with wirelessly powered electronic circuits |
JP5617532B2 (ja) * | 2010-10-29 | 2014-11-05 | ソニー株式会社 | 誘電サイトメトリ装置及び誘電サイトメトリによる細胞分取方法 |
JP5921829B2 (ja) | 2011-07-29 | 2016-05-24 | シャープ株式会社 | 捕集装置、分離方法、および表示方法 |
JP6114270B2 (ja) * | 2011-08-02 | 2017-04-12 | 東京エレクトロン株式会社 | 電界によりパターン及び構造形成を制御する方法及びデバイス |
EP2980558B1 (en) * | 2013-03-26 | 2019-11-06 | Sony Corporation | Measurement device and measurement method |
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US20210205822A1 (en) | 2021-07-08 |
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