JP6744542B2 - ラクトバチルス属乳酸菌の培養方法 - Google Patents
ラクトバチルス属乳酸菌の培養方法 Download PDFInfo
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- JP6744542B2 JP6744542B2 JP2016029147A JP2016029147A JP6744542B2 JP 6744542 B2 JP6744542 B2 JP 6744542B2 JP 2016029147 A JP2016029147 A JP 2016029147A JP 2016029147 A JP2016029147 A JP 2016029147A JP 6744542 B2 JP6744542 B2 JP 6744542B2
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Description
1)グラム陽性乳酸桿菌
2)ホモ型乳酸発酵
3)カタラーゼ陰性
4)芽胞形成能無し
5)好気条件下でも培養可
6)菌体外多糖類を産生
1)グラム陽性乳酸球菌
2)ホモ型乳酸発酵
3)カタラーゼ陰性
4)芽胞形成能 無し
5)好気条件下でも培養可
6)菌体外多糖類を産生
1)グラム陽性乳酸球菌
2)ホモ型乳酸発酵
3)カタラーゼ陰性
4)芽胞形成能 無し
5)好気条件下でも培養可
6)菌体外多糖類を産生
米タンパク「製品名プロファイン、ヤヱガキ醗酵技研(株)製」を2〜10%含み、必要に応じて炭素源としてグルコースまたはアラビノースを0.2〜5%付加したもの(残部は水)を加熱殺菌した培地にラクトバチルス・ラムノーサス(Lactobacillus rhamnosus)KO3株(NITE BP−771)を接種し、30〜45℃で24〜240時間培養後、デキストリン等の賦形剤を液中に加え乾燥、粉砕したものを乳酸菌発酵粉末として得た。
大豆タンパク「製品名ハイニュート、不二製油(株)製」を2〜10%含み、必要に応じて炭素源としてグルコースまたはアラビノースを0.2〜5%付加したもの(残部は水)を加熱殺菌した培地にラクトバチルス・ラムノーサス(Lactobacillus rhamnosus)KO3株(NITE BP−771)を接種し、30〜45℃で24〜240時間培養後、デキストリン等の賦形剤を液中に加え乾燥、粉砕したものを乳酸菌発酵粉末として得た。
培地として、10%脱脂粉乳、0.5%酵母エキスを加熱殺菌した培地、または、MRS培地にラクトバチルス・ラムノーサス(Lactobacillus rhamnosus)KO3株(NITE BP−771)を接種し、30〜45℃で24〜240時間培養後、デキストリン等の賦形剤を液中に加え乾燥、粉砕したものを乳酸菌発酵粉末として得た。
抗菌活性の指標として、ATP量を用い、以下の方法で評価した。各粉末の25%溶液100μlに対し,900μlのATP抽出試薬を入れ,室温で30分静置してATPを抽出した。各試料のATP量をATPアナライザー(TOA Electronics Ltd,Tokyo,Japan)を用いて計測した。なお,各条件につき同様の試料を各々8つずつ作製し,平均値±SDを算出した。
Claims (2)
- ラクトバチルス・ラムノーサス(Lactobacillus rhamnosus) KO3株を、米または大豆に由来するペプチドを0.1〜10重量%含有する培地で培養する工程を有するラクトバチルス属乳酸菌の培養方法。
- 培地が、さらに糖類を含む請求項1に記載のラクトバチルス属乳酸菌の培養方法。
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