JP6723409B2 - 分析チップ及び試料分析装置 - Google Patents
分析チップ及び試料分析装置 Download PDFInfo
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Description
本実施形態では、ELISA法による抗原抗体反応の化学発光を利用して、対象液体としての検体のアレルギー測定を行う生化学分析装置50及びこの生化学分析装置50に用いられる分析チップ10を本発明の試料分析装置及び分析チップの一例として説明する。
本実施形態の分析チップ10は、略円板状に形成される基板20と、基板20の中央に形成され、測定対象である対象液体が注入される注入口22と、注入口22から基板20の外縁まで放射状に形成され、毛細管現象を利用して対象液体を導入させることが可能な複数のマイクロ流路23と、を備え、それぞれのマイクロ流路23には、対象液体の成分に対し、選択的に反応する複数種類の抗原30が間隔を以て複数固定化される。これにより、1つのマイクロ流路23に複数種類の抗原30が固定化されているので、必要な対象液体の量を抑制しつつ、複数項目の測定を一度に行うことができる。また、マイクロ流路23が放射状に形成されているので、遠心力を利用してマイクロ流路23に残留する対象液体をマイクロ流路23から排出することができる。本実施形態のように、測定処理の過程で複数の種類の液体を注入、排液する処理を複数回繰り返すような試料分析において、特に有効である。
生化学分析装置50は、分析チップ10を設置可能なチップホルダ53と、チップホルダ53を回転させるチップホルダ回転ユニット54と、分析チップ10の注入口22に対象液体を注入する分注ユニット90と、対象液体と複数種類の抗原30のそれぞれの反応を一括して測定可能な測定ユニット80と、を備え、チップホルダ53をチップホルダ回転ユニット54によって回転させて、マイクロ流路23に導入した対象液体を排出する。これにより、強い表面張力を有する対象液体を、マイクロ流路23を用いて分析する分析装置にあって、迅速かつ確実に対象液体の排出を行うことができる。
12 下側ハウジング(ハウジング)
13 上側ハウジング(ハウジング)
16 液体捕捉空間
17 エア連通口(連通口)
18 開口部
20 基板
22 注入口
23 マイクロ流路(流路)
30 抗原(反応物質)
50 生化学分析装置(試料分析装置)
53 チップホルダ
54 チップホルダ回転ユニット(チップホルダ回転機構)
80 測定ユニット(測定装置)
90 分注ユニット(分注機構)
92 分注ノズル
95 ピペットチップ(先端部)
100 エアノズルユニット(エア注入機構)
110 制御ユニット(制御部)
210 分析チップ
216 液体捕捉空間
220 第1基板(基板)
222 注入口
230 第2基板(基板)
255 流路
Claims (4)
- 中央に形成される台部及び端面の全周にわたって形成される壁部を有する第1基板と、
中央に対象液体が注入される注入口が形成され、前記第1基板の上部に接合される第2基板と、
前記第1基板の前記台部の上面又は前記台部の上面に対向する前記第2基板の下面に固定化され、前記対象液体の成分に対して選択的に反応可能な複数種類の反応物質と、
を備え、
前記第2基板は、透光性の材料により形成されるとともに、前記注入口が平面視において前記台部よりも内側に収まっており、
前記台部の上面と前記第2基板の下面の間には、前記注入口に連通し、毛細管現象を利用して前記対象液体を前記反応物質に導く隙間が当該注入口の外周の全域に形成され、前記反応物質は前記隙間に位置する分析チップ。 - 前記分析チップの内部空間において、前記台部の外周面の周囲に形成される液体捕捉空間と、
前記液体捕捉空間に配置され、前記対象液体を吸収する吸収体と、を更に備える請求項1に記載の分析チップ。 - 前記吸収体は、前記台部の外周面を囲うリング状に形成される請求項2に記載の分析チップ。
- 請求項1から3までの何れかに記載の分析チップを設置可能なチップホルダと、
前記チップホルダを回転させるチップホルダ回転機構と、
前記分析チップの注入口に前記対象液体を注入する分注機構と、
前記対象液体と複数種類の前記反応物質のそれぞれの反応を測定可能な測定装置と、
を備え、
前記チップホルダを前記チップホルダ回転機構によって回転させて、前記隙間に導入した前記対象液体を排出する試料分析装置。
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US (1) | US10286394B2 (ja) |
EP (1) | EP3144682B1 (ja) |
JP (2) | JP6557656B2 (ja) |
DK (1) | DK3144682T3 (ja) |
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WO2017183298A1 (ja) * | 2016-04-20 | 2017-10-26 | シスメックス株式会社 | 核酸分析装置および核酸分析方法 |
CN108884430B (zh) * | 2016-04-20 | 2019-11-19 | 希森美康株式会社 | 核酸分析装置及核酸分析方法 |
JPWO2020195991A1 (ja) * | 2019-03-25 | 2020-10-01 | ||
JPWO2021015145A1 (ja) * | 2019-07-25 | 2021-01-28 | ||
WO2024082187A1 (zh) * | 2022-10-19 | 2024-04-25 | 深圳华大生命科学研究院 | 流体芯片移液设备及流体芯片的移液方法 |
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