JP6582050B2 - 分析チップ - Google Patents
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- JP6582050B2 JP6582050B2 JP2017539313A JP2017539313A JP6582050B2 JP 6582050 B2 JP6582050 B2 JP 6582050B2 JP 2017539313 A JP2017539313 A JP 2017539313A JP 2017539313 A JP2017539313 A JP 2017539313A JP 6582050 B2 JP6582050 B2 JP 6582050B2
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Description
感染症および他の内科的疾患は、継続的に人の生命に影響を及ぼす。例えば、患者の病気を診断するために、血液中または他の流体(体液)中の抗原の存在を検出するための開発が行われた。場合によっては、マイクロ流体デバイスを用いて、被検物質(検体)を分析する。
上述したように、マイクロ流体診断チップ(MDC)のようなマイクロ流体デバイスは、被検物質を含む流体を受け取り、流体内の病原体または他の異常を検出する。MDCを実現することに対する代案は、被検物質を含む流体のサンプル(試料)を採取して、分析のための研究室にそのサンプルを移送することである。この分析プロセスは、サンプルを適切に処理するために、デリケートで高価な試薬を用いて一連の正確な工程を行う技師を必要とする。試験を実行する人は通常、当該分析を行うために特別に訓練されている。
Claims (15)
- マイクロ流体分析チップであって、
主マイクロ流体ポンプを含む主マイクロ流体チャネルと、
前記主マイクロ流体チャネルから分岐し、その分岐した場所から下流側で前記主マイクロ流体チャネルに再び結合し、前記主マイクロ流体チャネルよりも小さい直径からなる二次マイクロ流体チャネルと、
前記二次マイクロ流体チャネル内の二次マイクロ流体ポンプとを含み、
前記二次マイクロ流体ポンプが、前記主マイクロ流体チャネルを通過する流体から第1のサイズの被検物質の粒子を引き寄せることができ、前記流体が、前記第1のサイズの被検物質の粒子、及び前記第1のサイズより大きいサイズの多数の粒子を含む、マイクロ流体分析チップ。 - 前記主マイクロ流体ポンプ及び前記二次マイクロ流体ポンプの活性化は交互に起こり、一方のマイクロ流体ポンプが活性化されている間に、他方のマイクロ流体ポンプは非活性化される、請求項1に記載のマイクロ流体分析チップ。
- 前記二次マイクロ流体チャネルが、そこを通過する前記第1のサイズの多数の粒子を計数するための第1のセンサを含む、請求項1又は2に記載のマイクロ流体分析チップ。
- 前記二次マイクロ流体チャネルの直径が、前記第1のサイズより大きいサイズの多数の粒子を除外する、請求項1〜3の何れか1項に記載のマイクロ流体分析チップ。
- 前記主マイクロ流体チャネルが、そこを通過する前記第1のサイズ及び前記第1のサイズより大きいサイズの被検物質の粒子を計数するための第2のセンサを含む、請求項1〜4の何れか1項に記載のマイクロ流体分析チップ。
- 前記第1のサイズの粒子の検出された数、及び前記第1のサイズより大きなサイズの粒子の検出された数を受け取り、前記第1のサイズの粒子の数を前記流体内の前記第1のサイズより大きなサイズの粒子と比較するための粒子差分モジュールを更に含む、請求項5に記載のマイクロ流体分析チップ。
- 前記主マイクロ流体ポンプ及び前記二次マイクロ流体ポンプがそれぞれ、前記第1のサイズの粒子を前記二次マイクロ流体チャネルへと押す及び引き寄せるように協働することができる、請求項1〜6の何れか1項に記載のマイクロ流体分析チップ。
- 分析チップであって、
粒子差分モジュールに通信可能に結合され得る第1のセンサを含む第1のマイクロ流体チャネルと、
前記第1のマイクロ流体チャネルから分岐し、その分岐した場所から下流側で前記第1のマイクロ流体チャネルに再び結合し、前記第1のマイクロ流体チャネルよりも小さい直径を有し、前記粒子差分モジュールに通信可能に結合され得る第2のセンサを含む第2のマイクロ流体チャネルとを含み、
前記第1のセンサは、前記第1のマイクロ流体チャネルを通過する流体内の被検物質の多数の粒子を計数し、
前記第2のセンサは、前記第2のマイクロ流体チャネルを通過する流体内の被検物質の多数の粒子を計数し、
前記粒子差分モジュールが、前記第1のマイクロ流体チャネルを通過した流体内の被検物質の粒子の数を、前記第2のマイクロ流体チャネル内の第1のマイクロ流体ポンプを用いて前記第2のマイクロ流体チャネルの中を通って引き寄せられた流体の被検物質の粒子の数と比較することができる、分析チップ。 - 前記第2のマイクロ流体チャネルは、前記第2のマイクロ流体チャネルの直径よりも大きい前記第1のマイクロ流体チャネルを通過した前記流体の被検物質の粒子の通過を妨げる、請求項8に記載の分析チップ。
- 前記第1のマイクロ流体チャネルが、被検物質の粒子を前記第1のマイクロ流体チャネルに送り出すための第2のマイクロ流体ポンプを含む、請求項8又は9に記載の分析チップ。
- 前記第1及び第2のマイクロ流体ポンプは、前記流体の粒子が前記第2のマイクロ流体チャネルの開口において蓄積しないように、前記開口により流体の直交流を生成するように協働する、請求項10に記載の分析チップ。
- 前記第1のマイクロ流体ポンプ及び前記第2のマイクロ流体ポンプの活性化は交互に起こり、一方のマイクロ流体ポンプが活性化されている間に、他方のマイクロ流体ポンプは非活性化される、請求項10又は11に記載の分析チップ。
- 前記第1及び第2のセンサがインピーダンスセンサである、請求項8〜12の何れか1項に記載の分析チップ。
- マイクロ流体チップ上の被検物質を分析する方法であって、
主マイクロ流体ポンプを用いて、被検物質の粒子を含む流体を、流体スロットに流体連結された主マイクロ流体チャネルに送り出し、
前記主マイクロ流体チャネルから分岐し、その分岐した場所から下流側で前記主マイクロ流体チャネルに再び結合し、前記主マイクロ流体チャネルよりも小さい直径からなる二次マイクロ流体チャネルへ、二次マイクロ流体ポンプを用いて前記被検物質の粒子を引き寄せることにより、前記二次マイクロ流体チャネルを通じて流体内の被検物質の粒子を選別することを含む、方法。 - 前記二次マイクロ流体チャネルを通過した被検物質の粒子の数の計数は、前記主マイクロ流体チャネル内のセンサを用いて主マイクロ流体チャネルを通過した流体内の全粒子の数の計数と比較される、請求項14に記載の方法。
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EP3582894A4 (en) | 2017-04-21 | 2020-03-04 | Hewlett-Packard Development Company, L.P. | MICROFLUIDIC CHIP |
WO2019156687A1 (en) * | 2018-02-12 | 2019-08-15 | Hewlett-Packard Development Company, L.P. | Microfluidic flow sensor |
WO2020032970A1 (en) * | 2018-08-10 | 2020-02-13 | Hewlett-Packard Development Company, L.P. | Serial cellular analytics |
US20220146400A1 (en) * | 2019-02-27 | 2022-05-12 | Kyocera Corporation | Particle separating and measuring device and particle separating and measuring apparatus |
KR20230115629A (ko) * | 2022-01-27 | 2023-08-03 | 스페클립스 주식회사 | 액체 정제 장치 및 이를 포함하는 진단 시스템 |
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AU2003224817B2 (en) * | 2002-04-01 | 2008-11-06 | Fluidigm Corporation | Microfluidic particle-analysis systems |
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