JP6544026B2 - Gas supply device of microorganism multiplication equipment - Google Patents

Gas supply device of microorganism multiplication equipment Download PDF

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JP6544026B2
JP6544026B2 JP2015092609A JP2015092609A JP6544026B2 JP 6544026 B2 JP6544026 B2 JP 6544026B2 JP 2015092609 A JP2015092609 A JP 2015092609A JP 2015092609 A JP2015092609 A JP 2015092609A JP 6544026 B2 JP6544026 B2 JP 6544026B2
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潤 武藤
潤 武藤
典充 金子
典充 金子
克明 松澤
克明 松澤
研吾 松尾
研吾 松尾
田中　浩
浩 田中
吉田　有子
有子 吉田
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IHI Corp
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Description

本発明は、微生物増殖設備のガス供給装置に関するものである。
TECHNICAL FIELD The present invention relates to a gas supply device of a microorganism growth facility.

従来、微生物増殖設備としては、例えば、培養槽に貯留された微生物としての微細藻類の培養液に対し、二酸化炭素（ＣＯ２）が含まれるガスを供給して気液接触させるものを挙げることができる。前記培養槽中にガスを通気させる方式としては、曝気管からガスを吹き出すものが一般的である（例えば、特許文献１参照）。 Conventionally, as a microorganism growth facility, for example, a device that supplies gas containing carbon dioxide (CO 2 ) to a culture solution of microalgae as microorganisms stored in a culture tank and makes it come into gas-liquid contact can be mentioned. it can. Generally as a system which aerates gas into the said culture tank, what blows off gas from an aeration pipe (for example, refer patent document 1).

前記微生物としての微細藻類は、微生物の粒子の大きさが数μｍ〜数ｍｍの単細胞性又は群体形成性の藻類であって、例えば、ユーグレナ、クロレラ、スピルリナ、ドナリエラ、ボツリオコッカス、シュードコリシスチス等である。   The microalga as the microorganism is a unicellular or colony forming algae whose particle size of the microorganism is several μm to several mm, and, for example, Euglena, Chlorella, Spirulina, Donaliella, Botryococcus, pseudocollis It is Chiss.

又、前記微生物としては、微細藻類以外にも、活性汚泥に含まれる好気性微生物、酵母、遺伝子組み換えした大腸菌等の有用物質を生産する細菌、抗生物質等の有用物質を生産する黴等の菌類、魚類の飼料として使用するワムシ等の原生動物を挙げることができる。これらの微生物を増殖させる場合、培養槽に貯留された微生物の培養液に対し、酸素（Ｏ２）が含まれるガス（空気）を曝気管から噴出させて気液接触させることが行われる。 Besides the microalgae, the microbes include aerobic microbes contained in activated sludge, bacteria producing useful substances such as yeast, genetically modified E. coli etc., fungi such as fungi producing useful substances such as antibiotics, etc. And protozoa such as rotifers used as feed for fish. In the case of growing these microorganisms, a gas (air) containing oxygen (O 2 ) is ejected from an aeration tube to bring the culture solution of the microorganisms stored in the culture tank into gas-liquid contact.

尚、前記微生物増殖設備と関連する一般的技術水準を示すものとしては、前記特許文献１以外にも、例えば、特許文献２がある。   In addition to Patent Document 1, for example, Patent Document 2 is an example showing a general technical level associated with the above-mentioned microorganism growth facility.

特開２０１３−８５４８８号公報JP, 2013-85488, A 特開２００５−２４６１８６号公報JP, 2005-246186, A

ところで、通常の微生物は、比重が水と等しいわけではないため、培養槽の培養液中における特に撹拌が弱い部分で沈降或いは浮上しがちである。   By the way, since ordinary microorganisms have a specific gravity which is not equal to that of water, they tend to settle or rise in a portion of the culture solution of the culture tank where the stirring is particularly weak.

前記微生物が培養槽の培養液中で沈降或いは浮上して偏在すると、増殖の反応に必要な溶存ガスや溶存固形物が微生物に対して充分且つ均一に供給されなくなる。このような不具合を防ぐためには、培養槽の内部全域において常にある一定以上の曝気量を維持しつつ撹拌強度を保つ必要があり、曝気量の削減には限界がある。   When the microorganism settles or floats in the culture solution of the culture tank and becomes unevenly distributed, the dissolved gas or dissolved solid necessary for the growth reaction can not be sufficiently and uniformly supplied to the microorganism. In order to prevent such a problem, it is necessary to maintain the stirring intensity while always maintaining a certain amount or more of aeration in the entire inside of the culture tank, and there is a limit to the reduction of the aeration amount.

しかしながら、曝気は培養槽の底部から気泡を噴出する必要があり、水頭差分を加圧した高圧ガスを曝気管へ供給しなければならないことから、前述の如く培養槽の内部全域において常にある一定以上の曝気量を維持しつつ撹拌強度を保つためには、大量の高圧ガスが必要となって電力消費量が増大し運転コストが嵩むという問題を有していた。   However, since aeration needs to eject air bubbles from the bottom of the culture tank, and high-pressure gas with a pressurized head difference must be supplied to the aeration tube, as described above, it is always more than a certain level throughout the inside of the culture tank. In order to maintain the agitation strength while maintaining the aeration amount, a large amount of high-pressure gas is required, which causes a problem that the power consumption increases and the operation cost increases.

本発明は、上記従来の問題点に鑑みてなしたもので、曝気量を減少させ、電力消費量を抑えて運転コスト削減を図り得る微生物増殖設備のガス供給装置を提供しようとするものである。
The present invention has been made in view of the above-mentioned conventional problems, and an object of the present invention is to provide a gas supply device of a microorganism breeding facility which can reduce the aeration amount and reduce the power consumption to reduce the operation cost. .

本発明は、培養槽に貯留された微生物の培養液に対し、ガスを供給して気液接触させつつ撹拌する曝気を行うことにより、微生物を増殖させる微生物増殖設備のガス供給装置において、
前記培養槽の底部に配設される一本の曝気管と、
該曝気管を前記培養槽の底部全域に亘って走査させる駆動機構と
を備え、
前記曝気管は、駆動機構が搭載された自走式の曝気管であることを特徴とする微生物増殖設備のガス供給装置にかかるものである。
 The present invention relates to a gas supply device of a microorganism growth facility that propagates microorganisms by performing aeration in which gas is supplied to a culture solution of microorganisms stored in a culture tank, and gas is supplied and agitated while being in gas-liquid contact.
A single aeration tube disposed at the bottom of the culture vessel;
A drive mechanism for scanning the aeration tube across the entire bottom of the culture vessel ;
The aeration tube is a self-propelled aeration tube on which a drive mechanism is mounted .

又、本発明は、培養槽に貯留された微生物の培養液に対し、ガスを供給して気液接触させつつ撹拌する曝気を行うことにより、微生物を増殖させる微生物増殖設備のガス供給装置において、
前記培養槽の底部を複数分割した各領域に配設される曝気管と、
該曝気管をそれぞれ前記培養槽の各領域全域に亘って走査させる駆動機構と
を備え、
前記曝気管は、駆動機構が搭載された自走式の曝気管であることを特徴とする微生物増殖設備のガス供給装置にかかるものである。
 Further , according to the present invention, there is provided a gas supply device of a microorganism growth facility for proliferating microorganisms by performing aeration in which gas is supplied to the culture solution of microorganisms stored in the culture tank to supply gas, and contact with gas and liquid.
An aeration tube disposed in each of a plurality of divided regions of the bottom portion of the culture vessel;
And a drive mechanism for scanning the aeration trachea over the entire region of the culture tank .
The aeration tube is a self-propelled aeration tube on which a drive mechanism is mounted .

前記微生物増殖設備のガス供給装置においては、前記曝気管から噴出されるガスの気泡を直径が１０μｍ〜１００μｍであるマイクロバブルとすることが好ましい。   In the gas supply device of the microorganism growth facility, it is preferable that the bubbles of the gas ejected from the aeration tube be microbubbles having a diameter of 10 μm to 100 μm.

前記微生物増殖設備のガス供給装置において、前記培養液に溶解させるガスは二酸化炭素であり、二酸化炭素濃度が空気より高いガスを使用することが好ましい。   In the gas supply device of the microorganism growth facility, the gas to be dissolved in the culture solution is carbon dioxide, and it is preferable to use a gas having a carbon dioxide concentration higher than that of air.

前記微生物増殖設備のガス供給装置において、前記培養液に溶解させるガスは酸素であり、酸素濃度が空気より高いガスを使用することが好ましい。   In the gas supply device of the microorganism growth facility, the gas to be dissolved in the culture solution is oxygen, and it is preferable to use a gas having an oxygen concentration higher than that of air.

本発明の微生物増殖設備のガス供給装置によれば、曝気量を減少させ、電力消費量を抑えて運転コスト削減を図り得るという優れた効果を奏し得る。
ADVANTAGE OF THE INVENTION According to the gas supply apparatus of the microorganism breeding installation of this invention, the aeration amount can be reduced, and the outstanding effect that operation | movement cost reduction can be achieved by suppressing electric power consumption can be show | played.

本発明の微生物増殖設備のガス供給装置の第一参考例を示す全体概要斜視図である。It is a whole general | schematic perspective view which shows the 1st reference example of the gas supply apparatus of the microorganism breeding installation of this invention. 本発明の微生物増殖設備のガス供給装置の第一参考例における曝気管からのガス噴出作動を示す側断面図であって、（ａ）は各組の第一番目の曝気管からガスが噴出した状態を示す図、（ｂ）は各組の第二番目の曝気管からガスが噴出した状態を示す図、（ｃ）は各組の第三番目の曝気管からガスが噴出した状態を示す図、（ｄ）は各組の第四番目の曝気管からガスが噴出した状態を示す図、（ｅ）は各組の第五番目の曝気管からガスが噴出した状態を示す図である。It is a sectional side view which shows the gas ejection action from the aeration pipe in the 1st reference example of the gas supply apparatus of the microorganism growth installation of this invention, Comprising: (a) is the gas which erupted from the first aeration pipe of each set Figure showing the state, (b) shows the state in which the gas is blown out from the second aeration tube of each set, (c) shows the state in which the gas is blown out from the third aeration tube in each set (D) is a figure which shows the state which gas squirted from the 4th aeration tube of each group, (e) is a figure which shows the state which gas squirted from the 5th aeration pipe of each group. 本発明の微生物増殖設備のガス供給装置の第二参考例を示す全体概要斜視図である。It is a whole schematic perspective view which shows the 2nd reference example of the gas supply apparatus of the microbial multiplication installation of this invention. 本発明の微生物増殖設備のガス供給装置の実施例を示す全体概要斜視図である。BRIEF DESCRIPTION OF THE DRAWINGS It is a whole outline schematic perspective view which shows the Example of the gas supply apparatus of the microbial multiplication installation of this invention. 本発明の微生物増殖設備のガス供給装置の実施例における自走式の曝気管を示す平断面図である。It is a plane sectional view showing the self-propelled aeration pipe in the example of the gas supply device of the microorganism breeding equipment of the present invention. 本発明の微生物増殖設備のガス供給装置の実施例における分岐給気管及び分岐給電ケーブルを示す断面図である。It is sectional drawing which shows the branch air supply pipe in the Example of the gas supply apparatus of the microbial multiplication installation of this invention, and a branch electric power feeding cable.

以下、本発明の実施の形態を添付図面を参照して説明する。   Hereinafter, embodiments of the present invention will be described with reference to the attached drawings.

図１及び図２は本発明の微生物増殖設備のガス供給装置の第一参考例である。前記微生物増殖設備は、培養槽１に貯留された微生物の培養液Ｃに対し、前記培養槽１の底部全域に亘って敷き詰められた曝気管２からガスを供給して気液接触させつつ撹拌することにより、微生物を増殖させるようになっている。
FIGS. 1 and 2 show a first reference example of a gas supply apparatus for a microorganism growth system of the present invention. The microorganism growth facility supplies gas to the culture solution C of the microorganism stored in the culture tank 1 from the aeration pipe 2 spread over the entire bottom of the culture tank 1, and stirs the gas while making liquid-liquid contact In some cases, they are designed to grow microorganisms.

第一参考例の場合、本数Ｎ（図の例ではＮ＝５）の曝気管２を一組の曝気ユニットＵとして、複数組（図の例では五組）の曝気ユニットＵが前記培養槽１の底部全域に亘って敷き詰められるようにしてある。但し、前記曝気管２の本数ＮはＮ＝５に限らず、又、前記曝気ユニットＵも五組に限定されるものではなく、培養槽１の規模に応じてそれぞれの数を増減し得ることは言うまでもない。
 In the case of the first reference example , a number N (N = 5 in the example of the drawing) aeration pipes 2 is taken as one aeration unit U, and aeration units U of plural sets (five sets in the example of the drawing) It is possible to spread all over the bottom of the However, the number N of the aeration tubes 2 is not limited to N = 5, and the number of aeration units U is not limited to five, and the number can be increased or decreased according to the scale of the culture tank 1 Needless to say.

前記曝気管２には、ブロワ３によって圧送されるガスが給気ヘッダ４から分岐給気管５を介して導入されるようになっている。尚、前記微生物が微細藻類である場合、前記ガスとしては、例えば、発電プラントから排出される二酸化炭素が含まれる排ガスを利用することができる。又、前記微生物が、微細藻類以外の、活性汚泥に含まれる好気性微生物、酵母、遺伝子組み換えした大腸菌等の有用物質を生産する細菌、抗生物質等の有用物質を生産する黴等の菌類、魚類の飼料として使用するワムシ等の原生動物である場合、前記ガスとしては、酸素が含まれる空気を用いれば良い。   The gas pumped by the blower 3 is introduced into the aeration tube 2 from the air supply header 4 via the branch air supply pipe 5. In addition, when the said microorganisms are micro algae, the waste gas in which the carbon dioxide discharged | emitted from a power plant is contained as said gas, for example can be utilized. Also, bacteria other than microalgae, bacteria that produce useful substances such as aerobic microorganisms contained in activated sludge, yeast, genetically modified E. coli etc., fungi such as fungi that produce useful substances such as antibiotics, etc. In the case of protozoa such as rotifers used as feed for the above, air containing oxygen may be used as the gas.

前記各分岐給気管５には、開閉弁６が設けられ、該開閉弁６は、制御器７から出力される制御信号７ａによって開閉されるようになっている。   Each of the branch air supply pipes 5 is provided with an on-off valve 6, which is opened and closed by a control signal 7a output from the controller 7.

前記制御器７は、曝気による撹拌がなされないときに微生物の偏在化が発生する時間をＴとした場合に、Ｔ／Ｎで表される時間だけ各曝気ユニットＵにおける一本の曝気管２の開閉弁６に対し制御信号７ａを出力し該曝気管２からガスを噴出させて停止するようになっている。この後、前記制御器７は、次の一本の曝気管２の開閉弁６に対し制御信号７ａを出力し該曝気管２からＴ／Ｎで表される時間だけガスを噴出させて停止する操作を行い、該操作を配設される曝気管２について繰り返し行わせるようになっている。尚、前記曝気管２の噴出孔（図示せず）は、曝気管２の長手方向へ等間隔に設けることが好ましいが、微生物の偏在化を抑制するために、前記噴出孔の配置、個数、孔径を適宜選定し得ることは言うまでもない。   The controller 7 sets a single aeration tube 2 of each aeration unit U for a time represented by T / N, where T is a time at which uneven distribution of microorganisms occurs when agitation by aeration is not performed. A control signal 7a is outputted to the on-off valve 6, and gas is ejected from the aeration tube 2 to stop. After that, the controller 7 outputs a control signal 7a to the on-off valve 6 of the next single aeration pipe 2 and spouts gas from the aeration pipe 2 for a time represented by T / N and stops it. An operation is performed, and the operation is repeatedly performed on the aeration tube 2 to be disposed. The ejection holes (not shown) of the aeration tube 2 are preferably provided at equal intervals in the longitudinal direction of the aeration tube 2, but in order to suppress uneven distribution of microorganisms, arrangement, number, and the like of the ejection holes. It goes without saying that the hole diameter can be selected appropriately.

次に、上記第一参考例の作用を説明する。
Next, the operation of the first reference example will be described.

前記制御器７からは各曝気ユニットＵにおける一本の曝気管２の開閉弁６に対し制御信号７ａが出力され、該開閉弁６の開閉が行われ、以下、全ての開閉弁６について開閉が順次行われていく。   A control signal 7a is outputted from the controller 7 to the on-off valve 6 of one aeration tube 2 in each aeration unit U, and the on-off valve 6 is opened or closed. It will be done sequentially.

これにより、先ず、図２（ａ）に示す如く、各曝気ユニットＵにおける第一番目の曝気管２からガスが噴出され、Ｔ／Ｎで表される時間経過後、第一番目の曝気管２からのガスの噴出が停止され、図２（ｂ）に示す如く、各曝気ユニットＵにおける第二番目の曝気管２からガスが噴出される。   As a result, first, as shown in FIG. 2 (a), gas is ejected from the first aeration pipe 2 in each aeration unit U, and after a time represented by T / N has elapsed, the first aeration pipe 2 The ejection of the gas from the nozzle is stopped, and the gas is ejected from the second aeration tube 2 in each aeration unit U, as shown in FIG. 2 (b).

続いて、Ｔ／Ｎで表される時間経過後、第二番目の曝気管２からのガスの噴出が停止され、図２（ｃ）に示す如く、各曝気ユニットＵにおける第三番目の曝気管２からガスが噴出され、Ｔ／Ｎで表される時間経過後、第三番目の曝気管２からのガスの噴出が停止され、図２（ｄ）に示す如く、各曝気ユニットＵにおける第四番目の曝気管２からガスが噴出される。   Subsequently, after a lapse of time represented by T / N, the blowout of gas from the second aeration pipe 2 is stopped, and as shown in FIG. 2C, the third aeration pipe in each aeration unit U is stopped. The gas is ejected from 2 and after a lapse of time represented by T / N, the ejection of the gas from the third aeration tube 2 is stopped, and as shown in FIG. 2 (d), the fourth in each aeration unit U The gas is emitted from the second aeration tube 2.

更に、Ｔ／Ｎで表される時間経過後、第四番目の曝気管２からのガスの噴出が停止され、図２（ｅ）に示す如く、各曝気ユニットＵにおける第五番目の曝気管２からガスが噴出され、Ｔ／Ｎで表される時間経過後、第五番目の曝気管２からのガスの噴出が停止され、図２（ａ）に示す如く、再び各曝気ユニットＵにおける第一番目の曝気管２からガスが噴出される。   Furthermore, after the lapse of time represented by T / N, the gas ejection from the fourth aeration pipe 2 is stopped, and as shown in FIG. 2 (e), the fifth aeration pipe 2 in each aeration unit U After the time when the gas is expelled and the time T / N elapses, the ejection of the gas from the fifth aeration tube 2 is stopped, and as shown in FIG. The gas is emitted from the second aeration tube 2.

以下、前述と同様に、図２（ａ）〜図２（ｅ）に示す操作が繰り返し行われる。   Hereinafter, in the same manner as described above, the operations shown in FIG. 2 (a) to FIG. 2 (e) are repeatedly performed.

ここで、撹拌強度がある一定の時間だけ弱まったとしても、即座に微生物の沈降或いは浮上が発生するわけではなく、撹拌強度が弱まった後、例えば５分〜２０分程度、撹拌がなされない場合にのみ、微生物の偏在化が発生する。   Here, even if the stirring strength is weakened for a certain period of time, sedimentation or floating of the microorganism does not occur immediately, and after stirring strength is weakened, for example, when stirring is not performed for about 5 minutes to 20 minutes. In only one, the uneven distribution of microorganisms occurs.

このため、曝気による撹拌がなされないときに微生物の偏在化が発生する時間Ｔが仮に５分である場合、Ｔ／Ｎ＝５／５＝１分だけ各曝気ユニットＵにおける一本の曝気管２の開閉弁６に対し制御器７より制御信号７ａが出力され該曝気管２からガスが噴出して停止する。この後、前記制御器７より次の一本の曝気管２の開閉弁６に対し制御信号７ａが出力され該曝気管２からＴ／Ｎ＝５／５＝１分だけガスが噴出して停止し、この操作が全ての曝気管２について繰り返し行われる。このようにすると、培養槽１の全域は、５分中、１分は曝気され撹拌されているので、微生物の偏在化は発生せず、増殖の反応に必要な溶存ガスや溶存固形物が微生物に対して充分且つ均一に供給され、供給不良になることはない。しかも、大量の高圧ガスを必要とせずに電力消費量が抑えられ運転コストが削減される。   For this reason, when the time T in which the uneven distribution of microorganisms occurs when the agitation by aeration is not performed is temporarily 5 minutes, one aeration tube 2 in each aeration unit U by T / N = 5/5 = 1 minute. A control signal 7a is outputted from the controller 7 to the on-off valve 6 and the gas is spouted from the aeration pipe 2 and stopped. Thereafter, a control signal 7a is outputted from the controller 7 to the on-off valve 6 of the next single aeration pipe 2, and gas is ejected from the aeration pipe 2 for T / N = 5/5 = 1 minute and stopped. This operation is repeated for all the aeration tubes 2. In this way, the whole area of the culture tank 1 is aerated and stirred for 1 minute in 5 minutes, so that uneven distribution of the microorganisms does not occur, and the dissolved gas and dissolved solids necessary for the reaction of growth are microorganisms. To be supplied sufficiently and uniformly, and there is no possibility of supply failure. In addition, power consumption can be reduced and operation costs can be reduced without requiring a large amount of high pressure gas.

因みに、従来のいわゆる間欠曝気では、前記曝気ユニットＵの本数Ｎ＝５の曝気管２から同時にガスを１分噴出させて４分停止する形となる。即ち、前記曝気ユニットＵが五組あると、曝気のためのブロワ３は、曝気管２を各組で五本ずつ、合計２５本分の曝気量を賄えるだけの容量のものを用意する必要があり、設備コストの増加は避けられない。これに対し第一参考例では、前記曝気ユニットＵが五組あっても、曝気のためのブロワ３は、曝気管２を各組で一本ずつ、合計五本分の曝気量を賄えるだけの容量のものを用意すれば済むため、設備コストの削減にもつながる。
Incidentally, in the conventional so-called intermittent aeration, gas is simultaneously ejected for one minute from the aeration tube 2 of the number N = 5 of the aeration unit U and stopped for four minutes. That is, when there are five sets of the aeration units U, blowers 3 for aeration need to have a capacity sufficient to cover the aeration amount of a total of 25 of the aeration pipes 2 in five sets of five each. There is an inevitable increase in equipment costs. On the other hand, in the first reference example , even if there are five sets of the aeration unit U, the blower 3 for aeration can only cover the aeration amount of a total of five, one for each set of the aeration tube 2. It will lead to the reduction of equipment cost because it is sufficient to prepare capacity.

こうして、曝気量を減少させ、電力消費量を抑えて運転コスト削減を図り得る。   Thus, the aeration amount can be reduced, the power consumption can be suppressed, and the operating cost can be reduced.

そして、前記本数Ｎの曝気管２を一組の曝気ユニットＵとして、複数組の曝気ユニットＵが前記培養槽１の底部全域に亘って敷き詰められるようにすることは、培養槽１の規模に応じて曝気を適切に行う上で有効となる。   The number N of aeration pipes 2 is regarded as a set of aeration units U so that a plurality of sets of aeration units U can be spread over the entire bottom of the culture tank 1 according to the scale of the culture tank 1. It is effective in properly performing aeration.

図３は本発明の微生物増殖設備のガス供給方法及び装置の第二参考例であって、図中、図１及び図２と同一の符号を付した部分は同一物を表わしている。
FIG. 3 is a second reference example of the gas supply method and apparatus of the microorganism growth facility of the present invention, and in the figure, the parts given the same reference numerals as in FIG. 1 and FIG. 2 represent the same things.

第二参考例の場合、前記培養槽１の底部を複数分割（図の例では五分割）した各領域に曝気管２を配設し、該曝気管２（図の例では五本）をそれぞれ前記培養槽１の各領域全域に亘って走査させる駆動機構８を設けた点を特徴としている。但し、前記培養槽１の底部の分割数は五分割に限定されるものではなく、培養槽１の規模に応じてその数を増減し得ることは言うまでもない。因みに、前記培養槽１の規模が小さい場合、該培養槽１の底部を複数の領域に分割せずに、前記曝気管２を一本のみ培養槽１の底部全域に亘って走査させるようにすることも可能である。
 In the case of the second reference example , the aeration trachea 2 is disposed in each area obtained by dividing the bottom of the culture tank 1 into a plurality of parts (five parts in the example of the drawing), and the aeration pipes 2 (five in the example of the drawing) It is characterized in that a drive mechanism 8 is provided which scans over the entire region of the culture tank 1. However, the number of divisions at the bottom of the culture tank 1 is not limited to five, and it goes without saying that the number can be increased or decreased according to the scale of the culture tank 1. Incidentally, when the scale of the culture vessel 1 is small, only one aeration tube 2 is scanned across the entire bottom of the culture vessel 1 without dividing the bottom of the culture vessel 1 into a plurality of regions. It is also possible.

前記培養槽１の底部には、その長手方向へ延びるガイドレール９を敷設し、該ガイドレール９上に前記曝気管２をスライドブロック１０を介して移動自在に配設してある。   A guide rail 9 extending in the longitudinal direction is laid at the bottom of the culture tank 1, and the aeration tube 2 is movably disposed on the guide rail 9 via a slide block 10.

前記駆動機構８は、前記培養槽１の長手方向両端における上縁部にウインチ１１，１２を設置すると共に、前記培養槽１の長手方向両端における底部にプーリ１３，１４を設置し、一方のウインチ１１から繰り出されるワイヤ１５を一方のプーリ１３に掛け回して前記培養槽１の底面に沿うように延ばし、該培養槽１の底面に沿うように延ばしたワイヤ１５を他方のプーリ１４に掛け回して他方のウインチ１２に巻き取るようにしてある。前記ワイヤ１５の中途部は、前記曝気管２に対しワイヤクランプ１６を介して接続するようにしてある。これにより、前記一方のウインチ１１からワイヤ１５を繰り出しつつ前記他方のウインチ１２でワイヤ１５を巻き取ると、前記曝気管２を培養槽１の底部における長手方向の一方向へスライドさせることができるようになっている。又、前記他方のウインチ１２からワイヤ１５を繰り出しつつ前記一方のウインチ１１でワイヤ１５を巻き取ると、前記曝気管２を培養槽１の底部における長手方向の反対方向へスライドさせることができるようになっている。   The drive mechanism 8 installs winches 11 and 12 at the upper edges at both ends in the longitudinal direction of the culture tank 1, and pulleys 13 and 14 at the bottoms at both ends in the longitudinal direction of the culture tank 1, and one winch A wire 15 fed out from 11 is wound around one pulley 13 and extended along the bottom surface of the culture tank 1, and a wire 15 extended along the bottom surface of the culture tank 1 is wound around the other pulley 14 It is wound on the other winch 12. The middle portion of the wire 15 is connected to the aeration tube 2 via a wire clamp 16. Thus, when the wire 15 is wound by the other winch 12 while the wire 15 is drawn out from the one winch 11, the aeration tube 2 can be slid in one longitudinal direction at the bottom of the culture tank 1 It has become. In addition, when the wire 15 is wound by the one winch 11 while drawing out the wire 15 from the other winch 12, the aeration tube 2 can be slid in the direction opposite to the longitudinal direction at the bottom of the culture tank 1. It has become.

尚、前記曝気管２には、ブロワ３によって圧送されるガスが給気ヘッダ４から分岐給気管５を介して導入されるようになっているが、前記曝気管２は移動するため、前記分岐給気管５はシリコンチューブ等のフレキシブルな配管としてある。   Although the gas pumped by the blower 3 is introduced into the aeration tube 2 from the air supply header 4 via the branch air supply pipe 5, the aeration tube 2 is moved, so the branch The air supply pipe 5 is a flexible pipe such as a silicon tube.

次に、上記第二参考例の作用を説明する。
Next, the operation of the second reference example will be described.

前記駆動機構８の一方のウインチ１１からワイヤ１５を繰り出しつつ他方のウインチ１２でワイヤ１５を巻き取ると、前記曝気管２は培養槽１の底部における長手方向の一方向（図３では右方向）へスライドする。又、前記他方のウインチ１２からワイヤ１５を繰り出しつつ前記一方のウインチ１１でワイヤ１５を巻き取ると、前記曝気管２は培養槽１の底部における長手方向の反対方向（図３では左方向）へスライドする。これにより、五本の曝気管２がそれぞれ前記培養槽１の各領域全域に亘って往復動する形となる。   When the wire 15 is wound from one winch 11 of the drive mechanism 8 while the wire 15 is wound by the other winch 12, the aeration tube 2 is one direction in the longitudinal direction at the bottom of the culture tank 1 (right direction in FIG. 3) Slide to Further, when the wire 15 is wound by the one winch 11 while drawing out the wire 15 from the other winch 12, the aeration tube 2 is in the direction opposite to the longitudinal direction at the bottom of the culture tank 1 (left direction in FIG. 3) Slide. As a result, the five aeration pipes 2 reciprocate over the entire region of the culture tank 1 respectively.

この間、前記ブロワ３によって圧送されるガスが給気ヘッダ４から分岐給気管５を介して曝気管２に導入され、該曝気管２からはガスが噴出している。これにより、培養槽１の全域は、往復動する曝気管２から噴出されるガスによって曝気され撹拌されているので、微生物の偏在化は発生せず、増殖の反応に必要な溶存ガスや溶存固形物が微生物に対して充分且つ均一に供給され、供給不良になることはない。しかも、大量の高圧ガスを必要とせずに電力消費量が抑えられ運転コストが削減される。又、曝気管２の本数も曝気ユニットＵ毎に一本ずつで済み、大幅な削減が可能となる。   During this time, the gas pumped by the blower 3 is introduced from the air supply header 4 to the aeration pipe 2 via the branch air supply pipe 5, and the gas is spouted from the aeration pipe 2. As a result, since the entire area of the culture tank 1 is aerated and stirred by the gas ejected from the reciprocating aeration pipe 2, uneven distribution of the microorganism does not occur, and the dissolved gas or dissolved solid necessary for the reaction of proliferation is generated. The material is sufficiently and uniformly supplied to the microbe, and there is no possibility of supply failure. In addition, power consumption can be reduced and operation costs can be reduced without requiring a large amount of high pressure gas. Further, the number of aeration pipes 2 may be one for each aeration unit U, and a significant reduction can be achieved.

更に、従来のいわゆる間欠曝気と比べた場合、第二参考例においても、曝気のためのブロワ３は、合計五本分の曝気管２からの曝気量を賄えるだけの容量のものを用意すれば済むため、設備コストの削減につながる。
Furthermore, in comparison with the conventional so-called intermittent aeration, also in the second reference example , if the blower 3 for aeration has a capacity sufficient to cover the aeration amount from the aeration tube 2 for a total of five tubes. This will lead to a reduction in equipment costs.

こうして、第二参考例においても、第一参考例と同様に、曝気量を減少させ、電力消費量を抑えて運転コスト削減を図り得る。
Thus, also in the second reference example , as in the first reference example , the aeration amount can be reduced, the power consumption can be suppressed, and the operating cost can be reduced.

そして、前記培養槽１の底部に配設される一本の曝気管２と、該曝気管２を前記培養槽１の底部全域に亘って走査させる駆動機構８とを備えることは、規模の小さい培養槽１に対して曝気を適切に行う上で有効となる。   And, a single aeration tube 2 disposed at the bottom of the culture vessel 1 and a drive mechanism 8 for scanning the aeration tube 2 across the entire bottom of the culture vessel 1 are small in scale. This is effective in appropriately aerating the culture vessel 1.

又、前記培養槽１の底部を複数分割した各領域に配設される曝気管２と、該曝気管２をそれぞれ前記培養槽１の各領域全域に亘って走査させる駆動機構８とを備えることは、大規模な培養槽１に対して曝気を適切に行う上で有効となる。   Also, an aeration tube 2 disposed in each region obtained by dividing the bottom portion of the culture vessel 1 into a plurality of parts, and a drive mechanism 8 for scanning the aeration tube 2 across the entire region of the culture vessel 1 are provided. Is effective in appropriately performing aeration on the large-scale culture tank 1.

図４〜図６は本発明の微生物増殖設備のガス供給方法及び装置の実施例であって、図中、図１〜図３と同一の符号を付した部分は同一物を表わしている。
4 to 6 is an embodiment of a gas supply method and apparatus for microbial growth apparatus of the present invention, in the figure, portions denoted by the same reference numerals as FIGS. 1-3 represent the same parts.

本実施例の場合、前記曝気管２を、駆動機構８Ｘが搭載された自走式の曝気管２とした点を特徴としている。
 In the case of the present embodiment, it is characterized in that the aeration trachea 2 is a self-propelled aeration trachea 2 on which a drive mechanism 8X is mounted.

前記曝気管２は、底部に走行車輪１７を備えた箱型の防水ケーシング１８の両側壁を貫通して両側方へ張り出すように延びている。   The aeration tube 2 extends through both side walls of a box-shaped waterproof casing 18 provided with a traveling wheel 17 at the bottom and protrudes to both sides.

本実施例における駆動機構８Ｘは、図５に示す如く、前記防水ケーシング１８の内部に、モータ１９と、減速機２０とを備え、前記モータ１９により減速機２０を介して走行車輪１７を回転駆動することにより、前記防水ケーシング１８から張り出す曝気管２を培養槽１の底面に沿って走行させるようになっている。
 As shown in FIG. 5, the drive mechanism 8X in the present embodiment includes a motor 19 and a reduction gear 20 inside the waterproof casing 18, and the traveling wheel 17 is rotationally driven by the motor 19 via the reduction gear 20. By doing this, the aeration tube 2 protruding from the waterproof casing 18 is made to travel along the bottom surface of the culture tank 1.

又、本実施例では、図４に示す如く、ベース配管２１から分岐するフレキシブルな分岐保護管２２が前記防水ケーシング１８に接続されている。前記ベース配管２１の内部には、ブロワ３から延びる給気ヘッダ４と、電源２３に接続された給電ケーブル２４とが挿入されている。前記分岐保護管２２の内部には、図４及び図６に示す如く、前記給気ヘッダ４から分岐して曝気管２に接続される分岐給気管５と、前記給電ケーブル２４から分岐してモータ１９に接続される分岐給電ケーブル２５とが挿入されている。これにより、前記曝気管２には、前記ブロワ３によって圧送されるガスが給気ヘッダ４から分岐給気管５を介して導入されると共に、前記モータ１９には、前記電源２３からの電気が給電ケーブル２４より分岐給電ケーブル２５を介して供給されるようになっている。
Further, in the present embodiment , as shown in FIG. 4, a flexible branch protection pipe 22 branched from the base pipe 21 is connected to the waterproof casing 18. An air supply header 4 extending from the blower 3 and a feeding cable 24 connected to a power supply 23 are inserted into the base pipe 21. As shown in FIGS. 4 and 6, a branch air supply pipe 5 branched from the air supply header 4 and connected to the aeration pipe 2 inside the branch protection pipe 22 and a motor branched from the power feeding cable 24 are provided. A branch feed cable 25 connected to 19 is inserted. Thereby, the gas pressure-fed by the blower 3 is introduced into the aeration tube 2 from the air supply header 4 via the branch air supply pipe 5, and the electric power from the power source 23 is supplied to the motor 19. It is supplied from the cable 24 via the branch feed cable 25.

尚、図４に示す実施例では、自走式の曝気管２を備えた曝気ユニットＵを五組配設しているが、培養槽１の規模に応じて前記曝気ユニットＵの数を増減し得ることは言うまでもない。因みに、前記培養槽１の規模が小さい場合、前記曝気ユニットＵを一組のみ設けて培養槽１の底部全域に亘って走査させるようにすることも可能である。
In the embodiment shown in FIG. 4, although five sets of aeration units U provided with a self-propelled aeration tube 2 are disposed, the number of the aeration units U may be increased or decreased according to the scale of the culture tank 1 It goes without saying that you get. Incidentally, when the scale of the culture tank 1 is small, it is also possible to provide only one set of the aeration unit U and scan the entire bottom of the culture tank 1.

次に、上記実施例の作用を説明する。
Next, the operation of the above embodiment will be described.

前記駆動機構８Ｘのモータ１９により減速機２０を介して走行車輪１７を正転させると、前記曝気管２が張り出す防水ケーシング１８は、培養槽１の底部における長手方向の一方向（図４では右方向）へ走行する。又、前記駆動機構８Ｘのモータ１９により減速機２０を介して走行車輪１７を逆転させると、前記曝気管２が張り出す防水ケーシング１８は、培養槽１の底部における長手方向の反対方向（図４では左方向）へスライドする。これにより、五本の曝気管２がそれぞれ前記培養槽１の各領域全域に亘って往復動する形となる。   When the traveling wheel 17 is rotated forward by the motor 19 of the drive mechanism 8X via the reduction gear 20, the waterproof casing 18 from which the aeration pipe 2 projects is one direction in the longitudinal direction at the bottom of the culture tank 1 (FIG. 4) Drive to the right) Further, when the traveling wheel 17 is reversed by the motor 19 of the drive mechanism 8X via the reduction gear 20, the waterproof casing 18 from which the aeration tube 2 overhangs has a direction opposite to the longitudinal direction at the bottom of the culture tank 1 (FIG. 4). Then slide to the left). As a result, the five aeration pipes 2 reciprocate over the entire region of the culture tank 1 respectively.

この間、前記ブロワ３によって圧送されるガスが給気ヘッダ４から分岐給気管５を介して曝気管２に導入され、該曝気管２からはガスが噴出している。これにより、培養槽１の全域は、往復動する曝気管２から噴出されるガスによって曝気され撹拌されているので、微生物の偏在化は発生せず、増殖の反応に必要な溶存ガスや溶存固形物が微生物に対して充分且つ均一に供給され、供給不良になることはない。しかも、大量の高圧ガスを必要とせずに電力消費量が抑えられ運転コストが削減される。又、曝気管２の本数も曝気ユニットＵ毎に一本ずつで済み、大幅な削減が可能となる。加えて、自走式の曝気管２とすることで、図３に示すガイドレール９、ウインチ１１，１２、プーリ１３，１４等の大規模な設備を不要とすることができる。   During this time, the gas pumped by the blower 3 is introduced from the air supply header 4 to the aeration pipe 2 via the branch air supply pipe 5, and the gas is spouted from the aeration pipe 2. As a result, since the entire area of the culture tank 1 is aerated and stirred by the gas ejected from the reciprocating aeration pipe 2, uneven distribution of the microorganism does not occur, and the dissolved gas or dissolved solid necessary for the reaction of proliferation is generated. The material is sufficiently and uniformly supplied to the microbe, and there is no possibility of supply failure. In addition, power consumption can be reduced and operation costs can be reduced without requiring a large amount of high pressure gas. Further, the number of aeration pipes 2 may be one for each aeration unit U, and a significant reduction can be achieved. In addition, by using the self-propelled aeration tube 2, large-scale equipment such as the guide rails 9, winches 11 and 12, and pulleys 13 and 14 shown in FIG. 3 can be eliminated.

更に、従来のいわゆる間欠曝気と比べた場合、本実施例においても、曝気のためのブロワ３は、合計五本分の曝気管２からの曝気量を賄えるだけの容量のものを用意すれば済むため、設備コストの削減につながる。
Furthermore, as compared with the conventional so-called intermittent aeration, in the present embodiment , it is sufficient to prepare a blower 3 for aeration having a capacity sufficient to cover the aeration amount from the aeration tube 2 for a total of five tubes. This leads to a reduction in equipment costs.

尚、前記防水ケーシング１８の内部に、小型のブロワとバッテリを搭載することも可能である。このようにすれば、図４に示すブロワ３、給気ヘッダ４、分岐給気管５、ベース配管２１、分岐保護管２２、電源２３、給電ケーブル２４、分岐給電ケーブル２５を不要とすることもできる。   It is also possible to mount a small blower and battery inside the waterproof casing 18. In this way, the blower 3, the air supply header 4, the branch air supply pipe 5, the base pipe 21, the branch protection pipe 22, the power supply 23, the power feeding cable 24, and the branch feeding cable 25 shown in FIG. .

こうして、本実施例においても、第一参考例及び第二参考例と同様に、曝気量を減少させ、電力消費量を抑えて運転コスト削減を図り得る。
Thus, also in the present embodiment , similarly to the first reference example and the second reference example , the aeration amount can be reduced, the power consumption can be suppressed, and the operating cost can be reduced.

一方、前記第一参考例、第二参考例、及び実施例において、曝気量そのものが低下することで、溶存ガスの供給速度も低下する可能性がある。これを補うためには、前記曝気管２から噴出されるガスの気泡を直径が１０μｍ〜１００μｍであるマイクロバブルとすることが有効となる。因みに、通常の気泡は、急激に培養液Ｃ中を上昇し最終的に液面で破裂する。しかし、マイクロバブルは気泡体積が微細であるため、上昇速度が遅く長時間、培養液Ｃ中に滞在し続ける。これにより、曝気量の低下に伴う溶存ガスの供給速度の低下を補うことが可能となる。
On the other hand, in the first reference example , the second reference example , and the example , when the aeration amount itself decreases, the supply rate of the dissolved gas may also decrease. In order to compensate for this, it is effective to use gas bubbles jetted from the aeration tube 2 as microbubbles having a diameter of 10 μm to 100 μm. By the way, normal air bubbles rapidly rise in the culture solution C and finally burst at the liquid surface. However, since the microbubbles have a fine bubble volume, the rising speed is slow and continues to stay in the culture solution C for a long time. This makes it possible to compensate for the decrease in the supply rate of the dissolved gas due to the decrease in the aeration amount.

又、前記微生物が微細藻類である場合、前記培養液Ｃに溶解させるガスは二酸化炭素となるが、二酸化炭素濃度が空気より高いガスを使用することが、曝気量の低下に伴う溶存ガスの供給速度の低下を補う上で有効となる。前記ガスとしては、例えば、発電プラントから排出される二酸化炭素が含まれる排ガスを利用することができる。   In addition, when the microorganism is a microalga, the gas to be dissolved in the culture solution C is carbon dioxide, but using a gas having a carbon dioxide concentration higher than that of the air leads to the supply of dissolved gas along with the decrease in aeration amount. It is effective in compensating for the decrease in speed. As the gas, for example, an exhaust gas containing carbon dioxide discharged from a power plant can be used.

更に又、前記微生物が、微細藻類以外の、活性汚泥に含まれる好気性微生物、酵母、遺伝子組み換えした大腸菌等の有用物質を生産する細菌、抗生物質等の有用物質を生産する黴等の菌類、魚類の飼料として使用するワムシ等の原生動物である場合、前記培養液Ｃに溶解させるガスは酸素となるが、酸素濃度が空気より高いガスを使用することが、曝気量の低下に伴う溶存ガスの供給速度の低下を補う上で有効となる。前記ガスとしては、例えば、酸素富化膜を通過させて酸素濃度を高めた空気を用いることができる。   Furthermore, the microbes may be aerobic microbes contained in activated sludge other than microalgae, bacteria producing useful substances such as yeast, genetically modified E. coli etc., fungi such as fungi producing useful substances such as antibiotics. In the case of protozoa such as rotifers used as feed for fish, the gas to be dissolved in the culture solution C is oxygen, but using a gas having an oxygen concentration higher than that of air causes the dissolved gas to be reduced along with the decrease in aeration amount. It is effective in compensating for the decrease in the supply rate of As the gas, for example, air in which the oxygen concentration is increased by passing through an oxygen enrichment film can be used.

尚、本発明の微生物増殖設備のガス供給装置は、上述の実施例にのみ限定されるものではなく、本発明の要旨を逸脱しない範囲内において種々変更を加え得ることは勿論である。
In addition, the gas supply apparatus of the microorganism growth installation of this invention is not limited only to the above-mentioned Example, Of course in the range which does not deviate from the summary of this invention, a various change can be added.

１ 培養槽
２ 曝気管
６ 開閉弁
７ 制御器
８ 駆動機構
８Ｘ 駆動機構
Ｃ 培養液
Ｕ 曝気ユニット DESCRIPTION OF SYMBOLS 1 culture tank 2 aeration pipe 6 on-off valve 7 controller 8 drive mechanism 8X drive mechanism C culture medium U aeration unit

Claims (5)

培養槽に貯留された微生物の培養液に対し、ガスを供給して気液接触させつつ撹拌する曝気を行うことにより、微生物を増殖させる微生物増殖設備のガス供給装置において、
前記培養槽の底部に配設される一本の曝気管と、
該曝気管を前記培養槽の底部全域に亘って走査させる駆動機構と
を備え、
前記曝気管は、駆動機構が搭載された自走式の曝気管であることを特徴とする微生物増殖設備のガス供給装置。 A gas supply device of a microorganism growth facility for growing microorganisms by performing aeration in which gas is supplied to the culture solution of the microorganism stored in the culture tank to supply gas, and the mixture is stirred while being brought into gas-liquid contact.
A single aeration tube disposed at the bottom of the culture vessel;
A drive mechanism for scanning the aeration tube across the entire bottom of the culture vessel ;
The gas supply device for a microorganism growth facility according to claim 1, wherein the aeration trachea is a self-propelled aeration trachea equipped with a drive mechanism . 培養槽に貯留された微生物の培養液に対し、ガスを供給して気液接触させつつ撹拌する曝気を行うことにより、微生物を増殖させる微生物増殖設備のガス供給装置において、
前記培養槽の底部を複数分割した各領域に配設される曝気管と、
該曝気管をそれぞれ前記培養槽の各領域全域に亘って走査させる駆動機構と
を備え、
前記曝気管は、駆動機構が搭載された自走式の曝気管であることを特徴とする微生物増殖設備のガス供給装置。 A gas supply device of a microorganism growth facility for growing microorganisms by performing aeration in which gas is supplied to the culture solution of the microorganism stored in the culture tank to supply gas, and the mixture is stirred while being brought into gas-liquid contact.
An aeration tube disposed in each of a plurality of divided regions of the bottom portion of the culture vessel;
And a drive mechanism for scanning the aeration trachea over the entire region of the culture tank .
The gas supply device for a microorganism growth facility according to claim 1, wherein the aeration trachea is a self-propelled aeration trachea equipped with a drive mechanism . 前記曝気管から噴出されるガスの気泡を直径が１０μｍ〜１００μｍであるマイクロバブルとした請求項１又は２記載の微生物増殖設備のガス供給装置。 The gas supply device of the microorganism growth facility according to claim 1 or 2, wherein the gas bubbles ejected from the aeration tube are microbubbles having a diameter of 10 μm to 100 μm. 前記培養液に溶解させるガスは二酸化炭素であり、二酸化炭素濃度が空気より高いガスを使用する請求項１〜３の何れか一項に記載の微生物増殖設備のガス供給装置。 The gas supply device of the microorganism growth facility according to any one of claims 1 to 3 , wherein the gas to be dissolved in the culture solution is carbon dioxide, and a gas having a carbon dioxide concentration higher than that of air is used. 前記培養液に溶解させるガスは酸素であり、酸素濃度が空気より高いガスを使用する請求項１〜３の何れか一項に記載の微生物増殖設備のガス供給装置。
The gas to be dissolved in the culture solution is oxygen, and a gas having an oxygen concentration higher than that of air is used. The gas supply device of the microorganism growth facility according to any one of claims 1 to 3 .
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