JP6487190B2 - 分子検出システム - Google Patents
分子検出システム Download PDFInfo
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- JP6487190B2 JP6487190B2 JP2014236193A JP2014236193A JP6487190B2 JP 6487190 B2 JP6487190 B2 JP 6487190B2 JP 2014236193 A JP2014236193 A JP 2014236193A JP 2014236193 A JP2014236193 A JP 2014236193A JP 6487190 B2 JP6487190 B2 JP 6487190B2
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L9/00—Supporting devices; Holding devices
- B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
- B01L9/527—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for microfluidic devices, e.g. used for lab-on-a-chip
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N11/00—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N11/00—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties
- G01N11/10—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties by moving a body within the material
- G01N11/16—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties by moving a body within the material by measuring damping effect upon oscillatory body
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N19/00—Investigating materials by mechanical methods
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0663—Stretching or orienting elongated molecules or particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/14—Means for pressure control
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- Chemical & Material Sciences (AREA)
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- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
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- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Nanotechnology (AREA)
- Fluid Mechanics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Description
M.Kumemura, D.Collard, S.Yoshizawa, D.Fourmy, N.Lafitte, S.Takeuchi, T.Fujii, L.Jalabert, and H.Fujita, "Direct bio-mechanical sensing of enzymatic reaction on DNA by silicon nanotweezers," Proc. IEEE MEMS‘10, pp. 915-918, 2010 N.Lafitte, M.Kumemura, L.Jalabert, D.Collard, and H.Fujita, "Real-time sensing of molecule binding on DNA with silicon nanotweezers," Proc. MicroTAS 2011, 389-391
16 先端部
30 微小液体装置
33 圧力制御微小液体ポンプ
36 微小液体流路
36a 入口部
36b 出口部
36c 開口部
37 液体
42 可動保持台
43 制御装置
Claims (9)
- 電極間に捕獲した分子の液体中の試薬との反応を検出する分子検出システムであって、
(a)一対の電極を備え、該電極の共振周波数及び振幅を計測可能な検出装置と、
(b)前記電極が通過可能な気液境界面が形成される開口部を側面に含む微小液体流路であって、該微小液体流路の入口部から出口部まで試薬の溶液を流すための微小液体流路を備える微小液体装置と、
(c)前記出口部に接続された圧力制御微小液体ポンプと、
(d)前記検出装置又は微小液体装置を移動可能に保持する可動保持台と、
(e)前記検出装置、圧力制御微小液体ポンプ及び可動保持台を制御する制御装置とを具備することを特徴とする分子検出システム。 - 前記検出装置は、前記電極間の間隔を所定の周波数で変化させることができる請求項1に記載の分子検出システム。
- 前記可動保持台が前記検出装置又は微小液体装置を移動させることによって、前記電極を、前記開口部に対して相対的に移動させ、前記気液境界面を通って、前記微小液体流路内の液体中に挿入すること及び該液体中から抜出することができる請求項1又は2に記載の分子検出システム。
- 前記制御装置は、事前に行われる位置決め操作によって、前記電極が前記液体中に位置する液中位置及び前記電極が前記液体外に位置する気中位置を決定して記憶する請求項3に記載の分子検出システム。
- 前記制御装置は、前記電極の振幅の変化に基づいて、前記液中位置及び気中位置を決定する請求項4に記載の分子検出システム。
- 前記圧力制御微小液体ポンプが前記微小液体流路内の液体を前記出口部から吸引排出することによって、他の液体が前記入口部から前記微小液体流路内に導入される請求項1〜5のいずれか1項に記載の分子検出システム。
- 前記制御装置は、前記微小液体流路内の液体を順次他の液体に交換させる請求項6に記載の分子検出システム。
- 前記制御装置は、分子を捕獲した電極を前記液体中に位置させた状態で、前記共振周波数及び振幅の計測を継続させ、計測結果を記憶する請求項7に記載の分子検出システム。
- 前記制御装置は、前記計測結果に基づいて前記分子の剛性又は減衰特性を算出する請求項8に記載の分子検出システム。
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2014236193A JP6487190B2 (ja) | 2014-11-21 | 2014-11-21 | 分子検出システム |
CA2968204A CA2968204A1 (en) | 2014-11-21 | 2015-11-20 | Molecule detecting system |
PCT/JP2015/082789 WO2016080543A1 (ja) | 2014-11-21 | 2015-11-20 | 分子検出システム |
EP15861543.5A EP3222993B8 (en) | 2014-11-21 | 2015-11-20 | Molecule detection system |
US15/527,866 US10843191B2 (en) | 2014-11-21 | 2015-11-20 | Molecule detecting system |
IL252363A IL252363A0 (en) | 2014-11-21 | 2017-05-18 | A system for detecting molecules |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2014236193A JP6487190B2 (ja) | 2014-11-21 | 2014-11-21 | 分子検出システム |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2016099219A JP2016099219A (ja) | 2016-05-30 |
JP6487190B2 true JP6487190B2 (ja) | 2019-03-20 |
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Application Number | Title | Priority Date | Filing Date |
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JP2014236193A Expired - Fee Related JP6487190B2 (ja) | 2014-11-21 | 2014-11-21 | 分子検出システム |
Country Status (6)
Country | Link |
---|---|
US (1) | US10843191B2 (ja) |
EP (1) | EP3222993B8 (ja) |
JP (1) | JP6487190B2 (ja) |
CA (1) | CA2968204A1 (ja) |
IL (1) | IL252363A0 (ja) |
WO (1) | WO2016080543A1 (ja) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3333566A1 (en) | 2016-12-12 | 2018-06-13 | Centre National de la Recherche Scientifique (CNRS) | Measuring system and manufacturing process of such a measuring system |
US11994484B2 (en) * | 2021-03-25 | 2024-05-28 | The Regents Of The University Of California | Apparatus and method for single cell discrimination |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2271179A1 (en) * | 1999-05-05 | 2000-11-05 | Sensorchem International Corporation | Process for monitoring and detecting small molecule - biomolecule interactions |
JP4834830B2 (ja) | 2005-05-09 | 2011-12-14 | 国立大学法人 東京大学 | レール分子固定方法及びナノ搬送デバイス |
WO2007016113A2 (en) * | 2005-07-27 | 2007-02-08 | Wisconsin Alumni Research Foundation | Nanoelectromechanical and microelectromechanical sensors and analyzers |
FR2894596B1 (fr) | 2005-12-13 | 2012-08-10 | Centre Nat Rech Scient | Procede d'autocalibration de biopuces |
US9134305B2 (en) * | 2009-02-04 | 2015-09-15 | The Trustees Of Boston College | Molecular imprinted nanosensors |
JP2012080791A (ja) | 2010-10-07 | 2012-04-26 | Univ Of Tokyo | 小児固形腫瘍または食道癌素因の評価方法及び評価キット、並びに、その薬剤及びその選択方法 |
WO2014018558A1 (en) * | 2012-07-23 | 2014-01-30 | Tasso, Inc. | Methods, systems, and devices relating to open microfluidic channels |
JP6090330B2 (ja) * | 2012-10-31 | 2017-03-08 | 日立化成株式会社 | センサチップ及び測定システム |
GB201220124D0 (en) | 2012-11-08 | 2012-12-26 | Provost Fellows Foundation Scholars And The Other Members Of Board Of | A microfluid device |
US9517464B2 (en) | 2012-12-05 | 2016-12-13 | Ian K. Glasgow | Dispensed liquid measurement device |
-
2014
- 2014-11-21 JP JP2014236193A patent/JP6487190B2/ja not_active Expired - Fee Related
-
2015
- 2015-11-20 US US15/527,866 patent/US10843191B2/en active Active
- 2015-11-20 EP EP15861543.5A patent/EP3222993B8/en active Active
- 2015-11-20 WO PCT/JP2015/082789 patent/WO2016080543A1/ja active Application Filing
- 2015-11-20 CA CA2968204A patent/CA2968204A1/en not_active Abandoned
-
2017
- 2017-05-18 IL IL252363A patent/IL252363A0/en unknown
Also Published As
Publication number | Publication date |
---|---|
WO2016080543A1 (ja) | 2016-05-26 |
EP3222993A1 (en) | 2017-09-27 |
EP3222993B8 (en) | 2020-02-26 |
EP3222993A4 (en) | 2018-08-01 |
CA2968204A1 (en) | 2016-05-26 |
IL252363A0 (en) | 2017-07-31 |
EP3222993A8 (en) | 2017-11-01 |
US10843191B2 (en) | 2020-11-24 |
EP3222993B1 (en) | 2019-12-11 |
JP2016099219A (ja) | 2016-05-30 |
US20190046981A1 (en) | 2019-02-14 |
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