JP6388365B2 - 魚類の防疫方法 - Google Patents
魚類の防疫方法 Download PDFInfo
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- JP6388365B2 JP6388365B2 JP2014063014A JP2014063014A JP6388365B2 JP 6388365 B2 JP6388365 B2 JP 6388365B2 JP 2014063014 A JP2014063014 A JP 2014063014A JP 2014063014 A JP2014063014 A JP 2014063014A JP 6388365 B2 JP6388365 B2 JP 6388365B2
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/60—Fish, e.g. seahorses; Fish eggs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Animal Behavior & Ethology (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Biotechnology (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
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- Alternative & Traditional Medicine (AREA)
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- Tropical Medicine & Parasitology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Insects & Arthropods (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Description
10〜30重量%であり、好ましくは15〜26重量%である。割合が10%未満であると効果が低下し、逆に50%を超えると成長不良や摂餌量低下となることがある。
〔実施例1及び2〕
1.飼料の調製
対照区として、魚粉(製品名フィッシュミール、日本水産株式会社製)を主体とする対照飼料を表1に示す組成で作製した。対照飼料の魚粉の20%及び30%を高蛋白菜種粕及び大豆粕で置換した飼料をそれぞれ実施例1及び2として作製した。高蛋白菜種粕及び魚粉の合計に対する高蛋白菜種粕の割合を、実施例1で17.2%とし、実施例2で25.0%とした。魚粉を高蛋白菜種粕で置換したことで減じた蛋白分を、大豆粕(製品名:大豆油粕、株式会社J-オイルミルズ製)の添加で補った。大豆粕及び魚粉の合計に対する大豆粕の割合は、実施例1で15.8%、そして実施例2で25%であった。
ビタミンMIX:βカロテン、ビタミンD3、ビタミンK3、ビタミンE、ビタミンB1、ビタミンB6、ビタミンB12、アスコルビン酸、ビオチン、イノシトール、ニコチン酸、パントテン酸、葉酸、塩化コリン、及びpアミノ安息香酸の混合物(コーキン化学株式会社製)
ミネラルMIX:NaCl,MgSO4・2H2O,NaHPO4・2H2O,KH2PO4,Fe Citrate,Ca lactate,Al(OH)3,ZnSO4・2H2O,CuSO4,MnSO4・5H2O,Ca(IO3)2,及びCoSO4・7H2Oの混合物(コーキン化学株式会社製)
Stay−C:アスコルビルモノフォスフェイト(DSM社製)
ヒラメの稚魚を表4に示す飼育環境で飼養した。
対照、比較例及び実施例の区において、それぞれ、飼料投与から2週間及び4週間後に、魚体重を測定した。さらに、摂餌量及び増重率を、以下の式により求めた。
飼料の投与前(Initial)、投与2週間(2W)に1尾、4週間(4W)では全生残個体サンプリングした。魚を解剖して、比肝重量、肝臓粗脂質、筋肉の粗蛋白質(CP)、粗脂質(CL)、灰分及び水分をケルダール法、ソックスレー法、直接灰化法及び常圧加熱乾燥法に従って求めた。
(1) 白血球の貪食率
飼料投与2週間(2W)及び4週間(4W)後に魚を捕獲し、白血球の貪食率を
白血球単離法(Percoll不連続密度勾配遠心法)に基づいて、下記式により算出した。
体表粘液の分泌が増加すると、主成分の多糖類が増加し、その結果、蛋白質量が低下する。多糖類の増加は、魚の防疫に寄与する。したがって、体表粘液中の蛋白質量は、魚の防疫能を示す指標となる。そこで、飼料投与2週(2W)及び4週(4W)後に魚を捕獲し、蛋白質量をLowry法に基づいて測定した。具体的には、以下の手順で活性を求めた。
血清の殺菌活性は、魚の防疫能を示す指標となる。そこで、飼料投与2週(2W)及び4週(4W)後に魚を捕獲し、血清の殺菌活性(E.coli生存率)をYamamotoらの方法(Yamamoto,A.et.al.,Fish Pathology,vol.30,1995,p123−124)に基づいて測定した。具体的には、以下の手順で活性を求めた。
Claims (11)
- 蛋白質含量が40〜50%の高蛋白菜種粕及び魚粉を含む飼料であって、前記高蛋白菜種粕及び前記魚粉の合計に対する前記高蛋白菜種粕の割合が10〜50重量%である前記飼料を魚類に投与して魚類の防疫能を強化することを含む、魚類の防疫方法。
- 前記防疫能が血清の殺菌活性である、請求項1に記載の魚類の防疫方法。
- 前記飼料が、さらに大豆粕を配合することを含む、請求項1又は2に記載の魚類の防疫方法。
- 前記大豆粕及び前記魚粉の合計に対する前記大豆粕の割合が、15〜30重量%である、請求項3に記載の魚類の防疫方法。
- 蛋白質含量が40〜50%であり、かつ粗繊維含量が1〜7%の高蛋白菜種粕及び魚粉を含む飼料であって、前記高蛋白菜種粕及び前記魚粉の合計に対する前記高蛋白菜種粕の割合が10〜50重量%である前記飼料を魚類に投与することを含む、魚の筋肉脂質を増大させる方法。
- 蛋白質含量が40〜50%であり、かつ粗繊維含量が1〜7%の高蛋白菜種粕及び魚粉を含み、前記高蛋白菜種粕及び前記魚粉の合計に対する前記高蛋白菜種粕の割合が10〜50重量%である養魚用飼料。
- さらに、大豆粕を含む、請求項6に記載の養魚用飼料。
- 前記大豆粕及び前記魚粉の合計に対する前記大豆粕の割合が、15〜30重量%である、請求項7に記載の養魚用飼料。
- 蛋白質含量が40〜50%であり、かつ粗繊維含量が1〜7%の高蛋白菜種粕及び魚粉を、前記高蛋白菜種粕及び前記魚粉の合計に対する前記高蛋白菜種粕の割合が10〜50重量%となるように配合することを含む、養魚用飼料の製造方法。
- さらに、大豆粕を配合することを含む、請求項9に記載の養魚用飼料の製造方法。
- 前記大豆粕及び前記魚粉の合計に対する前記大豆粕の割合が、10〜30重量%である、請求項10に記載の養魚用飼料の製造方法。
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JP2014063014A JP6388365B2 (ja) | 2014-03-26 | 2014-03-26 | 魚類の防疫方法 |
PCT/JP2015/057087 WO2015146581A1 (ja) | 2014-03-26 | 2015-03-11 | 魚類の防疫方法 |
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JP2014063014A JP6388365B2 (ja) | 2014-03-26 | 2014-03-26 | 魚類の防疫方法 |
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JP6388365B2 true JP6388365B2 (ja) | 2018-09-12 |
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KR102113974B1 (ko) * | 2018-11-12 | 2020-05-20 | 경기도 | 아쿠아포닉스 전용 사료 및 그 제조방법 |
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Publication number | Priority date | Publication date | Assignee | Title |
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JPS63301757A (ja) * | 1987-05-30 | 1988-12-08 | Meika Boeki Kk | 醗酵大豆粕を主原料とする養殖魚類用餌料 |
JPH03187349A (ja) * | 1989-12-15 | 1991-08-15 | Hayashikane Sangyo Kk | 抗菌性養魚飼料 |
JP3036920B2 (ja) * | 1991-09-26 | 2000-04-24 | 日清製粉株式会社 | 養魚用固形飼料 |
JP3919866B2 (ja) * | 1997-02-17 | 2007-05-30 | 昭和産業株式会社 | 菜種タンパク濃縮物及びそれを用いた海洋性養殖魚用 飼料 |
JP3970917B1 (ja) * | 2007-01-24 | 2007-09-05 | 株式会社J−オイルミルズ | 菜種ミールの製造方法 |
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WO2015146581A1 (ja) | 2015-10-01 |
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