JP6169423B2 - Algae culture lighting device - Google Patents
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Description
本発明は、藻類を培養する際に用いる藻類培養照明装置に関するものである。 The present invention relates to an algae culture lighting apparatus used when algae are cultured.
藻類とは、酸素発生型光合成を行う生物のうち、主に地上に生息する植物を除いた、単細胞生物から多細胞生物に至るまで進化的に全く異なるグループを含む便宜的な総称であるが、近年、炭化水素（原油相当物質）を生成する藻類等が発見されるなど藻類の有用性が再認識されるに至り、これらの藻類を効率よく培養することが様々な角度から研究されている。 Algae is a convenient generic name that includes evolutionary completely different groups from single-celled organisms to multicellular organisms, excluding plants that mainly inhabit the ground among organisms that perform oxygen-generating photosynthesis, In recent years, the usefulness of algae has been recognized again, such as the discovery of algae that produce hydrocarbons (crude oil equivalents), and the efficient cultivation of these algae has been studied from various angles.
しかし、藻類培養はいずれも研究段階のものがほとんどであり、確立された藻類培養装置も存在せず、各研究者は、フラスコ等の培養器、培養器の滅菌、培養液濃度、培養液温度、照射光量、光照射ＯＮ／ＯＦＦ時間（照射時間）、培養液攪拌速度、培養液通気量等の培養条件を、研究内容によって取捨選択し、条件に見合う装置を寄せ集めて使用しているのが現状である。
そのため、利便性が悪いうえ、培養のスピードが遅く、光合成に必要な光の照射量が不均一であるなど、藻類培養の再現性が保てず実験ごとの誤差が大きくなり、研究のスピードアップを図れないなどの問題があった。
However, almost all of the algae cultures are in the research stage, and there is no established algae culture device. Each researcher is responsible for incubators such as flasks, sterilization of incubators, culture medium concentrations, and culture medium temperatures. The culture conditions such as irradiation light quantity, light irradiation ON / OFF time (irradiation time), culture solution agitation speed, culture solution aeration amount, etc. are selected according to the research contents, and devices that meet the conditions are collected and used. Is the current situation.
For this reason, the reproducibility of algae culture cannot be maintained, such as the inconvenience, the culture speed is slow, and the amount of light required for photosynthesis is not uniform. There were problems such as being unable to plan.
従来の藻類培養装置としては、例えば、クリーンベンチ内で滅菌された５００ｍｌフラスコ（培養器）に、調製し濃度管理された培養液を入れ、フラスコの上部開放部に栓をし、このフラスコをシェイカーなどの振盪器上に設置して、振盪器の振幅により攪拌状態を調整するものが知られている。
また、培養液の温度管理は、これらの装置をインキュベータに入れ、インキュベータ内の空気の温度を制御することで、空気からの熱伝達で二次的に培養液の温度を一定にする方法が取られることが多かった。
As a conventional algae culture device, for example, a prepared and controlled culture solution is put into a 500 ml flask (incubator) sterilized in a clean bench, and the upper open part of the flask is stoppered. It is known that the agitation state is adjusted by the amplitude of the shaker.
In addition, the temperature control of the culture broth is performed by placing these devices in the incubator and controlling the temperature of the air in the incubator to make the temperature of the culture broth constant by heat transfer from the air. It was often done.
そして、このような藻類培養装置に用いられる照明装置としては、蛍光灯やＬＥＤ等の発光手段を、藻類を培養するフラスコの上方又は側方に配置して、光量調整を行いつつ、タイマコントロールにより任意時間にＯＮ／ＯＦＦ制御を行うものが一般的である。 And as an illuminating device used for such an algae culture device, light emitting means such as a fluorescent lamp and an LED are arranged above or to the side of a flask for cultivating algae, and the amount of light is adjusted by timer control. In general, an ON / OFF control is performed at an arbitrary time.
しかし、各培養器への光照射において側方から光を当てた場合は、光源側の培養器と遠方側の培養器とでは、受光量にかなりの差ができ、培養スピードにムラができるという問題点があり、上方から光を当てた場合は、光源面より幾ら均一な光を照射しても、培養器の口（上部開口）の蓋（栓）により光が遮蔽されるとともに、培養器の口部分周辺の容器の傾斜面により、光が反射・屈折してしまい減光されてしまうという問題点がある。
また、前記問題点を補うために、発光光量を大きくすると、光源の回路の発熱や輻射熱により培養器に影響が及び培養液に温度ムラができて好ましくないという問題もある。
However, when light is irradiated from the side in the light irradiation to each incubator, there is a considerable difference in the amount of light received between the incubator on the light source side and the incubator on the far side, and the culture speed can be uneven. There is a problem, and when light is applied from above, the light is shielded by the lid (plug) of the mouth (upper opening) of the incubator, even if the light is evenly irradiated from the light source surface. There is a problem that light is reflected and refracted and dimmed by the inclined surface of the container around the mouth portion.
In addition, if the amount of emitted light is increased in order to compensate for the above problem, there is also a problem that the incubator is affected by the heat generated by the circuit of the light source or radiant heat, and the culture medium is uneven in temperature, which is not preferable.
このような問題を解決するために、特許文献１には、光合成生物の培養装置において、シェイカーなどの振盪器の振動する基台２に人工光源９ａ〜９ｄを取り付けて、培養容器５の底面から光を照射する装置が開示されている（特許文献１の明細書の段落０００９，００１０、図面の図１，図２等参照）。 In order to solve such a problem, Patent Document 1 discloses that in a culture apparatus for photosynthetic organisms, artificial light sources 9a to 9d are attached to a base 2 that a shaker such as a shaker vibrates, and the culture vessel 5 is exposed from the bottom surface. An apparatus for irradiating light is disclosed (see paragraphs 0009 and 0010 of the specification of Patent Document 1 and FIGS. 1 and 2 of the drawings).
しかし、振動するものに人工光源等を取り付けた場合、その配線や回路等が振動により繰り返し疲労を起こして損傷してしまう可能性が高いうえ、培養液を温度制御するインキュベータ等についても同様に振動により耐久性に悪影響が生じる。また、通気用のチューブ等も振動してしまうため、安易で確実な固定もできないことから、些細な事故や破損等も懸念されるという問題もある。 However, when an artificial light source or the like is attached to a vibrating object, there is a high possibility that the wiring or circuit will be repeatedly fatigued and damaged by vibration, and the incubator that controls the temperature of the culture solution will also vibrate similarly. This adversely affects durability. In addition, since a ventilation tube or the like vibrates, it cannot be easily and surely fixed. Therefore, there is a problem that a minor accident or damage may occur.
そこでこの発明は、前記従来の問題を解決し、培養する藻類に影響する熱など、装置を稼働することによる培養環境の変動を極力小さくするとともに、培養の確実性、利便性、均一性を向上させることにより、実験や研究・開発等の再現性を向上させてスピードアップを図ることができ、耐久性、安全性、省エネルギー性にも優れた藻類培養照明装置を提供することを目的とする。 Therefore, the present invention solves the above-described conventional problems, minimizes fluctuations in the culture environment caused by operating the apparatus, such as heat that affects the algae to be cultured, and improves the reliability, convenience, and uniformity of the culture. It is an object of the present invention to provide an algae culture lighting device that can improve the reproducibility of experiments, research and development, and speed up, and is excellent in durability, safety, and energy saving.
前記課題を解決するために、請求項１に記載の発明は、最上部に配置され、藻類を培養する培養器に向け光を照射する光照射部と、この光照射部の下方に配置され、前記培養器内に挿置された攪拌子を回転させる駆動部と、を備えた藻類培養照明装置であって、前記光照射部は、上面に設置され、前記培養器を載置可能な拡散板と、この拡散板から下方に所定間隔離間した下面に設置された反射板と、これら拡散板と反射板との間の四周の側面に設置された発光手段と、を有する導光板タイプとなっており、前記駆動部で攪拌子を回転させて、前記拡散板の上に載置された前記培養器内の培養液を攪拌しつつ、前記発光手段から発せられる光を前記培養器の底面から該培養器内に直接照射することを特徴とする。
In order to solve the above-mentioned problem, the invention according to claim 1 is disposed at the uppermost part, and is disposed below the light irradiation unit, a light irradiation unit configured to irradiate light toward an incubator for culturing algae, An algae culture illumination device comprising: a drive unit that rotates a stirrer inserted in the incubator, wherein the light irradiation unit is installed on an upper surface, and a diffusion plate on which the incubator can be placed A light guide plate type comprising: a reflection plate installed on a lower surface spaced apart from the diffusion plate by a predetermined distance; and a light emitting means installed on a side surface of the four circumferences between the diffusion plate and the reflection plate. And rotating the stirrer in the drive unit to stir the culture medium in the incubator placed on the diffusion plate, and to emit light emitted from the light emitting means from the bottom surface of the incubator. Irradiation directly into the incubator .
請求項２に記載の発明は、請求項１に記載の藻類培養照明装置において、前記発光手段は、３原色の光を発する発光ダイオードがそれぞれ設けられた発光素子であり、発光する光の光量調整及び演色調整が可能となっている。 According to a second aspect of the present invention, in the algae culture lighting device according to the first aspect, the light emitting means is a light emitting element provided with light emitting diodes that emit light of three primary colors, and the light amount adjustment of the emitted light And color rendering adjustment is possible.
請求項３に記載の発明は、請求項１又は２に記載の藻類培養照明装置において、前記発光手段のＯＮ／ＯＦＦを切り換え自在なタイマを備える。 According to a third aspect of the present invention, in the algae culture lighting device according to the first or second aspect, a timer capable of switching on and off the light emitting means is provided.
請求項４に記載の発明は、請求項１ないし３のいずれかに記載の藻類培養照明装置において、前記駆動部は、複数のモータを有し、複数の攪拌子を回転可能となっている。 According to a fourth aspect of the present invention, in the algae culture lighting device according to any one of the first to third aspects, the drive unit has a plurality of motors and is capable of rotating a plurality of stirring bars.
請求項５に記載の発明は、請求項１ないし４のいずれかに記載の藻類培養照明装置において、前記駆動部は、攪拌子の回転数を設定可能となっている。 According to a fifth aspect of the present invention, in the algae culture lighting device according to any one of the first to fourth aspects, the drive unit can set the number of rotations of the stirrer.
この発明は、前記のようであって、請求項１に記載の発明によれば、最上部に配置され、藻類を培養する培養器に向け光を照射する光照射部と、この光照射部の下方に配置され、前記培養器内に挿置された攪拌子を回転させる駆動部と、を備えた藻類培養照明装置であって、前記光照射部は、上面に設置され、前記培養器を載置可能な拡散板と、この拡散板から下方に所定間隔離間した下面に設置された反射板と、これら拡散板と反射板との間の四周の側面に設置された発光手段と、を有する導光板タイプとなっており、前記駆動部で攪拌子を回転させて、前記拡散板の上に載置された前記培養器内の培養液を攪拌しつつ、前記発光手段から発せられる光を前記培養器の底面から該培養器内に直接照射するので、駆動部で培養液を一定の状態に攪拌しつつ、拡散板や反射板及び発光手段などの機能により均一な光を、培養器の底面に当接した状態で照射することができ、必要最小限の光を効率よく照射して藻類を効率よく培養することができ、省エネルギー性にも優れている。また、最短・最小距離で発光しつつも、発光により熱を発する発光手段が培養器から離れているため、直接培養器に熱伝達されることもないうえ、駆動部のモータなどで発生する熱も拡散板と反射板の間に存在する気体により断熱することができ、装置を稼働することによる培養環境の変動を極力小さくすることができる。このため、培養の確実性、利便性、均一性を向上させることができ、実験や研究・開発等の再現性を向上させて実験・研究・開発等のスピードアップを図ることができる。それに加え、振盪器を使用しなくても培養液を攪拌可能なため、培養液に連通するチューブ等の固定も容易で、耐久性、安全性にも優れている。
The present invention is as described above, and according to the invention described in claim 1, the light irradiation unit disposed at the top and irradiating light toward the incubator for culturing algae, and the light irradiation unit An algae culture illumination device that is disposed below and has a drive unit that rotates a stirrer inserted in the incubator, wherein the light irradiation unit is installed on an upper surface and mounts the incubator A diffusing plate that can be placed, a reflecting plate installed on the lower surface spaced apart from the diffusing plate by a predetermined distance, and a light emitting means installed on the four side surfaces between the diffusing plate and the reflecting plate. It is a light plate type, and the light emitted from the light emitting means is stirred while rotating the stir bar in the drive unit to stir the culture solution in the incubator placed on the diffusion plate. since the bottom surface of the vessel is irradiated directly into the incubator, a constant state of culture in the drive unit While stirring, uniform light can be emitted in contact with the bottom of the incubator by the functions of the diffusion plate, reflector, and light emitting means, and the algae can be efficiently irradiated with the minimum necessary light. It can be cultured efficiently and has excellent energy savings. In addition, since the light emitting means that emits light at the shortest and minimum distance but emits heat is separated from the incubator, heat is not directly transferred to the incubator, and heat generated by the motor of the drive unit, etc. Can also be insulated by the gas present between the diffuser and the reflector, and fluctuations in the culture environment due to operation of the apparatus can be minimized. Therefore, the certainty, convenience, and uniformity of culture can be improved, and the reproducibility of experiments, research, development, etc. can be improved to speed up experiments, research, development, etc. In addition, since the culture solution can be stirred without using a shaker, it is easy to fix a tube or the like communicating with the culture solution, and it is excellent in durability and safety.
請求項２に記載の発明によれば、請求項１に記載の藻類培養照明装置において、前記発光手段は、３原色の光を発する発光ダイオードがそれぞれ設けられた発光素子であり、発光する光の光量調整及び演色調整が可能となっているので、実験、研究等の再現性が非常に高いうえ、藻類の増殖と光量や波長などの関係が判断し易く、更に、実験、研究、開発などのスピードアップを図ることができる。 According to the invention described in claim 2, in the algae culture lighting device according to claim 1, the light emitting means is a light emitting element provided with light emitting diodes that emit light of three primary colors, respectively. Light quantity adjustment and color rendering adjustment are possible, so the reproducibility of experiments, research, etc. is very high, and it is easy to judge the relationship between the growth of algae and the light quantity, wavelength, etc. In addition, experiments, research, development etc. You can speed up.
請求項３に記載の発明によれば、請求項１又は２に記載の藻類培養照明装置において、前記発光手段のＯＮ／ＯＦＦを切り換え自在なタイマを備えるので、実験、研究等の再現性が非常に高いうえ、藻類の増殖と日照時間などの関係が判断し易く、更に、実験、研究、開発などのスピードアップを図ることができる。 According to the invention described in claim 3, since the algal culture lighting device according to claim 1 or 2 is provided with a timer capable of switching ON / OFF of the light emitting means, the reproducibility of experiments, research, etc. is extremely high. In addition, it is easy to determine the relationship between the growth of algae and sunshine duration, and it is possible to speed up experiments, research and development.
請求項４に記載の発明によれば、請求項１ないし３のいずれかに記載の藻類培養照明装置において、前記駆動部は、複数のモータを有し、複数の攪拌子を回転可能となっているので、同時に複数の培養器において藻類の増殖の実験、研究、開発等することができ、更に、実験、研究、開発などのスピードアップを図ることができる。 According to invention of Claim 4, in the algae culture lighting apparatus in any one of Claim 1 thru | or 3, the said drive part has a some motor, and can rotate a some stirring element. Therefore, it is possible to conduct experiments, research and development of algae in a plurality of incubators at the same time, and to speed up experiments, research and development.
請求項５に記載の発明によれば、請求項１ないし４のいずれかに記載の藻類培養照明装置において、前記駆動部は、攪拌子の回転数を設定可能となっているので、強く回転させることにより藻類が回転の衝撃で壊れてしまうようなことを防ぐことができるうえ、培養効率がよい回転数（攪拌状態）を判断することができ、実験、研究等の再現性を高め、実験、研究、開発などのスピードアップを図ることができる。 According to the invention described in claim 5, in the algae culture lighting device according to any one of claims 1 to 4, since the drive unit can set the number of rotations of the stirrer, it is rotated strongly. In addition to preventing the algae from being broken by the impact of rotation, it is possible to determine the number of rotations (stirring state) with good culture efficiency, improve the reproducibility of experiments, research, etc. Speed up research and development.
図１〜図７を用いて、本発明の一実施の形態である実施例に係る藻類培養照明装置について説明する。 The algae culture lighting apparatus according to an example which is an embodiment of the present invention will be described with reference to FIGS.
図１及び図２に示す、符号１が実施例に係る藻類培養照明装置１であり、この藻類培養照明装置１は、最上部に設けられ、フラスコなどの培養器Ｆ内の微細藻類（図７参照）に光を照射する光照射部２と、この光照射部２の直下に設けられ、後述の攪拌子３０を駆動する駆動部３と、下部に設けられ、これらを制御する制御部４などが、本装置の筺体としてメラミン樹脂が焼き付け塗装された鋼板からなる装置本体１０内に収容された装置であり、光合成で増殖する光合成微細藻類（いわゆる光合成微生物）の培養に用いられる照明装置としての機能に加え、マグネットスターラーの機能を兼ね備えた装置である。 1 and 2, reference numeral 1 denotes an algae culture illumination device 1 according to the embodiment. The algae culture illumination device 1 is provided at the top, and the microalgae in the incubator F such as a flask (FIG. 7). A light irradiating unit 2 that irradiates light, a drive unit 3 that is provided directly below the light irradiating unit 2 and drives a stirrer 30 described later, and a control unit 4 that is provided below and controls them. Is a device housed in a device body 10 made of a steel plate baked and coated with melamine resin as a casing of the device, and as a lighting device used for culturing photosynthetic microalgae (so-called photosynthetic microorganisms) that grow by photosynthesis In addition to the function, it is a device that combines the functions of a magnetic stirrer.
光照射部２は、図１ないし図４に示すように、上面に設けられた透明なアクリル板からなる幅３３８ｍｍ×奥行き３６２ｍｍ程度の大きさの矩形の拡散板２１と、下面に設けられた拡散板２１と略同形の内面に反射面が形成されたアクリル板からなる反射板２２と、これら拡散板２１と反射板２２との間の四周の側面に設けられた発光手段であるＬＥＤ発光素子２０などからなり、このＬＥＤ発光素子２０が一辺１８個計７２個配列された導光板タイプのＬＥＤ照明であり、培養器Ｆの底面から培養器Ｆに収容された培養液Ｂ中の光合成微細藻類（図１、図７参照）に向け最大で光量子束密度９０μｍｏｌ／ｍ２／ｓの光を照射する機能を有している。
なお、拡散板２１の上に培養器Ｆを載置する関係上、所望の曲げ強度を確保するため拡散板２１の下（拡散板２１と反射板２２との間）に透明なアクリル板を挿置しても構わない。
As shown in FIGS. 1 to 4, the light irradiation unit 2 includes a rectangular diffusion plate 21 having a width of about 338 mm and a depth of about 362 mm made of a transparent acrylic plate provided on the upper surface, and a diffusion provided on the lower surface. A reflective plate 22 made of an acrylic plate having a reflective surface formed on the inner surface substantially the same shape as the plate 21, and an LED light emitting element 20, which is a light emitting means provided on the four side surfaces between the diffuser plate 21 and the reflective plate 22. This is a light guide plate type LED illumination in which a total of 72 LED light emitting elements 20 are arranged in a total of 18 sides. The photosynthetic microalgae in the culture solution B contained in the incubator F from the bottom of the incubator F ( It has a function of irradiating light with a photon flux density of 90 μmol / m 2 / s at the maximum (see FIGS. 1 and 7).
In order to secure the desired bending strength, a transparent acrylic plate is inserted under the diffusion plate 21 (between the diffusion plate 21 and the reflection plate 22) in order to place the incubator F on the diffusion plate 21. It does not matter.
このＬＥＤ発光素子２０は、３ｍｍ角のパッケージ内に光の三原色であるＲ（赤）、Ｇ（緑）、Ｂ（青）の発光ダイオード（ＬＥＤ）がそれぞれ封じられた発光ユニットであり、三原色の発光ダイオードは、それぞれ波長のピークがＲ（赤）＝６６０ｎｍ、Ｇ（緑）＝５２０ｎｍ、Ｂ（青）＝４４５ｎｍで図５に示す発光スペクトルの出力範囲で発光するダイオードとなっている。このため、各ＬＥＤ発光素子２０は、後述の制御部４により光量調整して任意の色彩の光に演色可能となっている。 This LED light emitting element 20 is a light emitting unit in which light emitting diodes (LEDs) of R (red), G (green), and B (blue) which are three primary colors of light are sealed in a 3 mm square package. The light emitting diodes emit light in the output spectrum output range shown in FIG. 5 with wavelength peaks of R (red) = 660 nm, G (green) = 520 nm, and B (blue) = 445 nm. For this reason, each LED light emitting element 20 can perform color rendering on light of an arbitrary color by adjusting the amount of light by the control unit 4 described later.
このように、光照射部２は、照射する光の波長や照射光量を任意に設定できるうえ、上面の拡散板２１と下面の反射板２２の機能により、発光面である上面から均一な光を照射することができる。
また、培養器Ｆの直下（即ち、対象物との距離が０）から光を照射するので、光の反射や拡散による無駄が殆どなく、微細藻類の培養に必要な最低限の光を発光するだけで済み、発光効率、省エネルギー性が良いだけでなく、遮るものや支障となるものが存在しないため、実験、研究等の再現性が非常に高く、実験、研究、開発などのスピードアップを図ることができる。
As described above, the light irradiation unit 2 can arbitrarily set the wavelength of light to be irradiated and the amount of light to be irradiated, and can also emit uniform light from the upper surface, which is a light emitting surface, by the functions of the diffusion plate 21 on the upper surface and the reflection plate 22 on the lower surface. Can be irradiated.
Moreover, since light is irradiated from directly under the incubator F (that is, the distance to the object is 0), there is almost no waste due to reflection and diffusion of light, and the minimum light necessary for culturing microalgae is emitted. This is not only good for light emission efficiency and energy saving, but also has no obstacles or obstacles, so the reproducibility of experiments and research is very high, and the speed of experiments, research and development is increased. be able to.
そのうえ、もともと発光量が必要最小限であるうえ、発光による発熱の少ない発光ダイオードを光源としているため、発光による発熱の影響が少ないうえ、導光板タイプのＬＥＤ照明であるため、熱源となる発光ダイオードのチップが上面の四周（周囲）にしか存在せず、光照射の対象物である培養器Ｆから遠く、更に、光照射部２から培養液Ｂへの伝熱の影響が少ない。 In addition, since the light source is originally a light emitting diode that emits less light and generates less heat due to light emission, it is less affected by light emission, and because it is a light guide plate type LED illumination, it is a light emitting diode that serves as a heat source. This chip is present only on the four circumferences (periphery) of the upper surface, is far from the incubator F that is the object of light irradiation, and is less affected by heat transfer from the light irradiation unit 2 to the culture medium B.
駆動部３は、図４に示す（１）〜（４）の位置に、ブラシレスモータなどの回転速度が可変式のモータ（図示せず）が４つ設けられており、磁石からなる攪拌子３０を回転駆動して同時に４つの培養器Ｆを攪拌可能となっている。また、このモータは、単相１００Ｖ５０／６０Ｈｚの電源で駆動し、後述の制御部４を介して、ＰＷＭ制御等で制御され、回転数が１００〜１０００ｒｐｍの範囲で任意に設定可能となっている。
なお、攪拌子３０は、棒磁石をフッ素樹脂などの樹脂で覆ったものであり、モータの数に応じて４個装備されている（図１、図４参照）。
The drive unit 3 is provided with four motors (not shown) having a variable rotational speed such as a brushless motor at the positions (1) to (4) shown in FIG. The four incubators F can be stirred at the same time. Further, this motor is driven by a single-phase 100V 50/60 Hz power source, and is controlled by PWM control or the like via the control unit 4 described later, and can be arbitrarily set within a range of rotation speed of 100 to 1000 rpm. .
The stirrer 30 is a bar magnet covered with a resin such as a fluororesin, and is provided with four pieces according to the number of motors (see FIGS. 1 and 4).
このため、駆動部３によれば、強く回転させることにより藻類が回転の衝撃で壊れてしまうようなことを防ぐことができるうえ、培養効率がよい回転数（攪拌状態）を判断することができ、実験、研究等の再現性を高め、実験、研究、開発などのスピードアップを図ることができる。
また、培養器Ｆ内の攪拌子３０のみが回ることにより培養液Ｂを攪拌することができるので、いかようにも安易で確実なチューブ等の固定ができ、チューブによるバブリング通気も可能となる（図１参照）。
さらに、従来の振盪器のように、振動によるチューブ外れもなく、些細な事故や破損のおそれもない。
For this reason, according to the drive part 3, it can prevent that algae breaks with the impact of rotation by rotating strongly, and can determine the rotation speed (stirring state) with sufficient culture efficiency. , Increase the reproducibility of experiments and research, and speed up experiments, research and development.
In addition, since only the stirring bar 30 in the incubator F can rotate, the culture solution B can be stirred, so that the tube can be fixed easily and surely, and bubbling aeration by the tube is also possible ( (See FIG. 1).
Furthermore, unlike the conventional shaker, the tube does not come off due to vibration, and there is no risk of minor accidents or breakage.
制御部４は、前述の光照射部２と駆動部３を制御する機能を有し、装置本体１０の正面パネルがこの制御部４の操作パネル４０となっている。この操作パネル４０は、図６に示すように、上部左に、各撹拌子３０の回転数を表示する回転数表示計、その右横に前述の４つのモータの回転数を調節する調節つまみが設けられている。 The control unit 4 has a function of controlling the light irradiation unit 2 and the drive unit 3 described above, and the front panel of the apparatus main body 10 is an operation panel 40 of the control unit 4. As shown in FIG. 6, the operation panel 40 has, on the upper left, a rotation speed indicator that displays the rotation speed of each stirring bar 30, and an adjustment knob that adjusts the rotation speeds of the four motors on the right side. Is provided.
また、操作パネル４０の下部には、一番左に全体の電源スイッチが、その右隣に照明をＯＮ／ＯＦＦするスイッチが設けられており、中央に、発光ダイオードの３原色の発光比率を０〜１００％で調整するとともに発光光量を調整できるＬＥＤ調整器が設けられ、右に光照射部２の発光時間を１５分毎に２４時間設定可能な照明タイマが設けられている。 Further, at the lower part of the operation panel 40, an entire power switch is provided on the leftmost side, and a switch for turning on / off illumination is provided on the right side, and the light emission ratio of the three primary colors of the light emitting diodes is set to 0 in the center. An LED adjuster that can adjust the amount of emitted light while adjusting at ˜100% is provided, and an illumination timer that can set the light emission time of the light irradiation unit 2 every 15 minutes for 24 hours is provided on the right.
このため、制御部４によれば、容易に照射する光の光量や波長及び色彩を任意に設定することができるとともに、攪拌の状況も任意に設定することができる。
よって、藻類増殖の環境設定が容易で実験や研究対象のパラメータの差異が明確となるうえ、実験、研究等の再現性を高め、実験、研究、開発などのスピードアップを図ることができる。
For this reason, according to the control part 4, while being able to set arbitrarily the light quantity, wavelength, and color of the light to irradiate easily, the condition of stirring can also be set arbitrarily.
Therefore, the environment setting for algae growth is easy and the difference in the parameters of the experiment and research object becomes clear, the reproducibility of the experiment and research can be improved, and the speed of the experiment, research and development can be increased.
以上のように、本実施例に係る藻類培養照明装置１によれば、光照射部２として導光板タイプの光源を採用することにより、光源である発光面自体の照射ムラを極限まで抑えることができるだけでなく、培養器Ｆ直下の底面より直接照射する構成により、各培養器Ｆへの照射ムラがなくなり、均一性・再現性が高められる。 As described above, according to the algae culture lighting apparatus 1 according to the present embodiment, by adopting a light guide plate type light source as the light irradiation unit 2, it is possible to suppress the irradiation unevenness of the light emitting surface itself as a light source to the limit. Not only can the irradiation be performed directly from the bottom surface immediately below the incubator F, but uneven irradiation to each incubator F can be eliminated, and uniformity and reproducibility can be improved.
また、培養器Ｆ直下の底面より直接照射するので、照射対象物である培養器Ｆに接触した状態、即ち、ゼロ距離照射により照射するため、距離が離れることによる光の拡散の無駄もなく効率よく照射し、光合成の利用効率も高くすることができる。そのため、コストパフォーマンスも向上する。 In addition, since the direct irradiation is performed from the bottom surface directly under the incubator F, the irradiation is performed in a state of contact with the incubator F that is an irradiation target, that is, irradiation by zero distance irradiation. Irradiation can be performed well, and the utilization efficiency of photosynthesis can be increased. Therefore, cost performance is also improved.
それに加え、光照射部２には、拡散板２１と反射板２２との間に、対流でしか熱の移動が起こらないため熱伝達率が極めて小さい空気などの気体が存在するため（図３参照）、駆動部３が発する熱も確実に遮ることができ、装置からの伝熱の影響を極力排除して培養液Ｂの温度の安定化を図ることができる。
よって、培養器Ｆ中の培養液Ｂの温度管理を極めて容易に、且つ厳密にすることができ、実験、研究、開発などのスピードアップを更に図ることができる。
In addition, in the light irradiation unit 2, heat transfer occurs only by convection between the diffusion plate 21 and the reflection plate 22, and therefore there is a gas such as air having a very low heat transfer coefficient (see FIG. 3). ), The heat generated by the drive unit 3 can also be reliably blocked, and the temperature of the culture medium B can be stabilized by eliminating the influence of heat transfer from the apparatus as much as possible.
Therefore, the temperature control of the culture solution B in the incubator F can be made extremely easily and strictly, and the speed of experiments, research, development, etc. can be further increased.
また、インキュベータ等を利用する場合でも、装置本体１０及び培養器Ｆの全てが静止状態に保つことができるため、インキュベータに伝達される振動がなく、固定方法が複雑になることもなく、インキュベータ等の耐久性、寿命には全く影響を与えることがない。
そのうえ、４連、８連等、一括培養をしても各培養器Ｆにおいて同条件の培養が可能であり、再現性・均一性も飛躍的に向上し、結果、スクリーニング等の実験も可能となる。
Further, even when using an incubator or the like, since all of the apparatus main body 10 and the incubator F can be kept stationary, there is no vibration transmitted to the incubator, and the fixing method is not complicated. It has no effect on the durability and life of the product.
In addition, even in batch cultures such as 4 or 8 cells, the same conditions can be cultured in each incubator F. The reproducibility and uniformity are dramatically improved, and as a result, experiments such as screening are possible. Become.
以上のように、本実施例に係る藻類培養照明装置を説明したが、図面で示した各構成部材の形状や構造、材質等も、あくまでも好ましい一例を示すものであり、特許請求の範囲内で適宜設計変更が可能であることは云うまでもない。
特に、発光時間を１５分毎に設定できるタイマを例示して説明したが、秒単位に設定可能としても構わないし、別途の制御プログラム等によりパソコンなどの電気計算機等から発光時間を任意に設定でき、且つ、発光手段のパルス時間やデューティー比を設定できるようにしても良い。また、駆動部のモータの数には制限はない。
As described above, the algae culture lighting device according to the present embodiment has been described. However, the shape, structure, material, and the like of each component shown in the drawings are only preferable examples, and are within the scope of the claims. Needless to say, the design can be changed as appropriate.
In particular, a timer that can set the light emission time every 15 minutes has been described as an example, but it may be set in units of seconds, and the light emission time can be arbitrarily set from an electric computer such as a personal computer by a separate control program or the like. In addition, the pulse time and duty ratio of the light emitting means may be set. Moreover, there is no restriction | limiting in the number of the motors of a drive part.
１ 藻類培養照明装置
２ 光照射部
２０ ＬＥＤ発光素子（発光手段）
２１ 拡散板
２２ 反射板
３ 駆動部
３０ 攪拌子
４ 制御部
４０ 操作パネル
DESCRIPTION OF SYMBOLS 1 Algae culture lighting apparatus 2 Light irradiation part 20 LED light emitting element (light emission means)
21 Diffuser 22 Reflector 3 Drive unit 30 Stirrer 4 Control unit 40 Operation panel
Claims (5)
前記光照射部は、上面に設置され、前記培養器を載置可能な拡散板と、この拡散板から下方に所定間隔離間した下面に設置された反射板と、これら拡散板と反射板との間の四周の側面に設置された発光手段と、を有する導光板タイプとなっており、
前記駆動部で攪拌子を回転させて、前記拡散板の上に載置された前記培養器内の培養液を攪拌しつつ、前記発光手段から発せられる光を前記培養器の底面から該培養器内に直接照射することを特徴とする藻類培養照明装置。 A light irradiating unit disposed at the top and irradiating light toward an incubator for culturing algae; and a driving unit disposed below the light irradiating unit and rotating a stirrer inserted in the incubator. An algal culture lighting device comprising:
The light irradiation unit is installed on the upper surface, a diffusion plate on which the incubator can be placed , a reflection plate installed on the lower surface spaced apart from the diffusion plate by a predetermined distance, and the diffusion plate and the reflection plate It is a light guide plate type having light emitting means installed on the sides of the four circumferences between,
The stirrer is rotated by the driving unit to stir the culture medium in the incubator placed on the diffusion plate, and to emit light emitted from the light emitting means from the bottom of the incubator. An algae culture lighting device characterized by direct irradiation inside .
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