JP6165876B2 - レスベラトロール生合成イネ及びその用途 - Google Patents
レスベラトロール生合成イネ及びその用途 Download PDFInfo
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- JP6165876B2 JP6165876B2 JP2015547868A JP2015547868A JP6165876B2 JP 6165876 B2 JP6165876 B2 JP 6165876B2 JP 2015547868 A JP2015547868 A JP 2015547868A JP 2015547868 A JP2015547868 A JP 2015547868A JP 6165876 B2 JP6165876 B2 JP 6165876B2
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- rice
- resveratrol
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- synthase gene
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Description
本発明のレスベラトロール合成酵素遺伝子を利用して、レスベラトロールを高濃度で生合成するイネを開発するために、レスベラトロールを高濃度で生合成するラッカセイのレスベラトロール合成酵素遺伝子を分離した。
正方向プライマー
5'-ATGGTGTCTGTGAGTGGAATTC-3 '(配列番号2)
逆方向プライマー
5'-CGTTATATGGCCACACTGC-3 '(配列番号3)
実施例1で得られたレスベラトロール合成酵素遺伝子をイネに挿入するために形質転換用ベクターを作製した。
実施例2で製造したイネ形質転換用ベクターのpSB2220をイネに挿入するために、凍結融解(freezing-thawing)法(非特許文献4)を用いて、 アグロバクテリウム・ツメファシエンス(A. tumefaciens)(LBA 4404)に挿入した。
4-1.遺伝子の単純挿入有無の確認
実施例3で製造された形質転換イネのレスベラトロール合成酵素遺伝子が挿入されているか否かをPCRをによって確認した。
実施例3で製造された本発明のレスベラトロール生合成形質転換イネにおけるレスベラトロール合成酵素遺伝子のイネゲノム内の位置を確認するために制限酵素処理した後、アダプタを取り付けてPCRを行い、その産物の塩基配列を解析して確認した。
実施例3で製造された本発明のレスベラトロール生合成形質転換イネに対する挿入遺伝子のゲノムDNA上の挿入位置及び挿入周辺の塩基配列を確認するために隣接したDNAの配列解析を行った。本発明の形質転換イネに二つのT-DNAが挿入された事実とその周辺の塩基配列及び挿入されたT-DNAの構造を分析した。結論として、二つのT-DNAがイネの染色体の12番目の同一位置に互いに向かい合う方向に挿入されていることを確認した。
実施例3で製造された形質転換イネでにおいて、レスベラトロールが実際に生成されるかについてHPLC分析によって確認した。
実施例6-1.露地栽培条件での田植え時期に応じたイネの生育特性
本発明のレスベラトロール生合成イネの田植えを、露地栽培条件で6月1日、6月15日、6月30日に行った結果、田植え後の葉の発育終了までは、6月1日の田植えでは田植え後75.1日、6月15日の田植えでは65.1日、6月30日の田植えでは55.1日の期間を要し、この時期の積算温度はそれぞれ1,886℃、1,688℃、1,474℃であった。この際、全開した葉の数は、それぞれ13.0葉、11.6葉、11.2葉で、6月1日に田植えを行った場合、出穂期が一般の中晩生種の品種よりも4〜5日遅れるという傾向を示した(図11)。
高温条件での本発明のレスベラトロール生合成イネの出穂期は、露地条件の6月1日の田植えに比べ(8月22日出穂)、温度が上昇するにつれて出穂が早くなり、3.5℃の温度上昇条件で出穂が5日早くなるという結果を示した。
本発明のレスベラトロール生合成イネの田植え時期及び高温条件に応じた米の量の変化を測定した。
本発明のレスベラトロール生合成イネの田植え時期に応じたレスベラトロール含有量の変化を測定した。
本発明のレスベラトロール生合成イネの田植え時期、栽培温度、収穫時期に応じたレスベラトロール及びピセイドの種子又は植物体内の含有量の変化を測定した。
本発明のレスベラトロール生合成イネの栽培環境に応じた種子内(玄米)のレスベラトロールの含有量を測定した。
本発明のレスベラトロール生合成イネの農業的特性を解明するために、本発明のレスベラトロール生合成イネと形質転換母品種であるドンジンイネを比較品種として、農業的特性を調査した。
本発明のレスベラトロールを高濃度に含む米が代謝性疾患に及ぼす影響を確認するために、長期間の高脂肪食餌方法により、代謝性疾患が誘導された動物モデル(マウス)を用いて確認した。具体的には、C57BL/6 近交系マウス(inbred mouse)各15匹に12週間高脂肪の食事を与え、糖尿病、肥満、高脂血症及び高コレステロール症などの代謝性疾患モデルを確立した。その後、形質転換イネ又はドンジンイネの種子を用いて作製した飼料を実験動物に12週間給与した。12週間給与した後、3週間に一回ずつ尾静脈から採血して血糖、コレステロールなどを分析し、代謝性疾患の動物モデルにおけるレスベラトロール生合成米、形質転換イネの効能を確認した。
前述のとおり、実験動物の飼料において炭水化物の部分をドンジンイネとレスベラトロール含有形質転換イネで代替した飼料を、代謝性疾患が誘導されたマウスに与えた後、血糖を測定した(図2)。
前述のとおり、実験動物の飼料の炭水化物の部分をドンジンイネとレスベラトロール含有形質転換イネで代替した飼料を、代謝性疾患が誘導されたマウスに与えた後、血中の総コレステロールの量、血中の総中性脂肪の量、血中LDLコレステロールの量及び血中のHDLコレステロールの量を測定した(図3)。
前述のとおり、実験動物の飼料において、炭水化物の部分をドンジンイネとレスベラトロール含有形質転換イネで代替した飼料を、代謝性疾患が誘導されたマウスに与えた後、体重の変化及び総脂肪量を測定した(図4)。
このようにして製造されたさらに別のレスベラトロール生合成イネの効能を、実施例7と同様の方法により代謝性疾患誘導動物で確認した。
前述のとおり、実験動物の飼料において、炭水化物の部分をドンジンイネとレスベラトロール含有イネ比較群形質転換イネで代替した飼料を、代謝性疾患が誘導されたマウスに与えた後、血糖を測定した(図5)。
前述のとおり、実験動物の飼料において、炭水化物の部分をドンジンイネとレスベラトロール含有イネの比較群の形質転換イネで代替した飼料を、代謝性疾患が誘導されたマウスに与えた後、血中の総コレステロール量、血中の総中性脂肪量、血中LDLコレステロール量と血中のHDLコレステロール量を測定した(図6〜図9)。
前述のとおり、実験動物の飼料において、炭水化物の部分をドンジンイネとレスベラトロール含有イネ比較群形質転換イネで代替した飼料を、代謝性疾患が誘導されたマウスに与えた後、体重の変化を測定した(図10)。
Claims (14)
- 天然型イネの12番目の染色体に存在する配列番号7の塩基配列の572番塩基の後に2コピーのレスベラトロール合成酵素(resveratrol synthase)遺伝子が連続的に発現可能に、互いに向かい合う方向に挿入されて、レスベラトロールを生合成するイネ。
- 前記レスベラトロール合成酵素遺伝子が、ラッカセイ(Arachis hypogaea)由来である、請求項1に記載のイネ。
- 前記レスベラトロール合成酵素遺伝子が、配列番号1の塩基配列で構成されたものである、請求項1に記載のイネ。
- 前記イネは、受託番号KCTC12529BPである、請求項1に記載のイネ。
- 前記イネは、種子完熟時に玄米の場合2〜5.3μg/g、白米の場合1.8〜4.1μg/gのレスベラトロールを含有するものである、請求項1に記載のイネ。
- ピセイド(piceid)をさらに生合成する、請求項1に記載のイネ。
- 前記イネは、種子完熟時に、白米の場合1.0〜6.5μg/gのピセイドを含有するものである、請求項6に記載のイネ。
- 請求項1〜7のいずれかに記載のイネから得られる米。
- 天然型イネの12番目の染色体に存在する配列番号7の塩基配列の572番塩基の後に2コピーのレスベラトロール合成酵素遺伝子が発現可能に、互いに向かい合う方向に挿入されて、レスベラトロールを生合成するイネから得られる米を含む、代謝性疾患の予防及び改善のための健康機能食品組成物。
- 前記天然型イネが、天然型ドンジン米(Dongin rice)である、請求項9に記載の健康機能食品組成物。
- 前記レスベラトロールを生合成するイネが、ピセイドをさらに生合成するものである、請求項9に記載の健康機能食品組成物。
- 前記代謝性疾患が、肥満、糖尿病、高脂血症及び高コレステロール症からなる群から選択される、請求項9に記載の健康機能食品組成物。
- 天然型イネの12番目の染色体に存在する配列番号7の塩基配列の572番塩基の後に2コピーのレスベラトロール合成酵素遺伝子が発現可能に、互いに向かい合う方向に挿入されて、レスベラトロールを生合成するイネから得られる米を含む、代謝性疾患の予防や改善のための動物飼料組成物。
- 天然型イネの12番目の染色体に存在する配列番号7の塩基配列の572番塩基の後に2コピーのレスベラトロール合成酵素遺伝子が発現可能に、互いに向かい合う方向に挿入されて、レスベラトロールを生合成するイネから得られる米を含む、代謝性疾患の予防及び改善のための薬学的組成物。
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