JP6129382B1 - Method for measuring cuticle peeling amount, and method for evaluating the degree of hair damage using the method - Google Patents
Method for measuring cuticle peeling amount, and method for evaluating the degree of hair damage using the method Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 38
- 230000003700 hair damage Effects 0.000 title claims abstract description 8
- 210000004209 hair Anatomy 0.000 claims abstract description 42
- 239000000126 substance Substances 0.000 claims abstract description 21
- 238000012800 visualization Methods 0.000 claims abstract description 13
- 239000010419 fine particle Substances 0.000 claims abstract description 9
- 238000012545 processing Methods 0.000 claims description 9
- 238000003384 imaging method Methods 0.000 claims description 7
- 239000002245 particle Substances 0.000 claims description 5
- 238000011156 evaluation Methods 0.000 claims description 2
- 230000032798 delamination Effects 0.000 claims 1
- 230000001680 brushing effect Effects 0.000 abstract description 5
- 239000002657 fibrous material Substances 0.000 abstract description 2
- 238000005259 measurement Methods 0.000 description 10
- 230000006378 damage Effects 0.000 description 6
- 238000004140 cleaning Methods 0.000 description 2
- 230000003749 cleanliness Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002203 pretreatment Methods 0.000 description 2
- 229940057950 sodium laureth sulfate Drugs 0.000 description 2
- SXHLENDCVBIJFO-UHFFFAOYSA-M sodium;2-[2-(2-dodecoxyethoxy)ethoxy]ethyl sulfate Chemical compound [Na+].CCCCCCCCCCCCOCCOCCOCCOS([O-])(=O)=O SXHLENDCVBIJFO-UHFFFAOYSA-M 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 102000011782 Keratins Human genes 0.000 description 1
- 108010076876 Keratins Proteins 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 210000001520 comb Anatomy 0.000 description 1
- 238000010410 dusting Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012883 sequential measurement Methods 0.000 description 1
- 239000013077 target material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
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Abstract
【課題】毛髪等のキューティクルを表面に有する繊維状物質から、ブラッシング等の外的刺激によって、その都度剥離するキューティクルの量を測定する方法、及び該方法を用いて毛髪の損傷度を評価する方法を提供する。【解決手段】クリーンルーム内において、被測定物質に外的刺激を与える工程と、外的刺激により前記被測定物質より剥離するキューティクルを微粒子可視化システムによって光学的に捕捉し、前記キューティクルの剥離量を計測する工程と、を含むことを特徴とするキューティクルの剥離量の測定方法。【選択図】図１A method for measuring the amount of a cuticle that peels off each time from an external stimulus such as brushing from a fibrous material having a cuticle such as hair on the surface, and a method for evaluating the degree of hair damage using the method I will provide a. In a clean room, an external stimulus is applied to a substance to be measured, and a cuticle peeled off from the substance to be measured by the external stimulus is optically captured by a fine particle visualization system, and the amount of the cuticle peeled is measured. And a step of measuring the amount of peeling of the cuticle. [Selection] Figure 1
Description
本発明はキューティクル剥離量の測定方法、及び該方法を用いた毛髪の損傷度の評価方法に関する。 The present invention relates to a method for measuring the amount of cuticle peeling, and a method for evaluating the degree of hair damage using the method.
従来、キューティクルの剥離量に関する測定方法として、外的刺激を与える前後の毛髪の外径を測定することによってキューティクルの剥離量を推定する方法、水中で毛髪をブラッシングし、回収した残液から遠心分離によって剥離したキューティクルを回収する方法等が知られている（特許文献１、２参照）。 Conventionally, as a method of measuring the amount of peeling of the cuticle, a method of estimating the amount of peeling of the cuticle by measuring the outer diameter of the hair before and after applying an external stimulus, brushing the hair in water and centrifuging from the collected residual liquid A method of recovering the cuticle peeled by the method is known (see Patent Documents 1 and 2).
しかしながら、外的刺激によって剥離するキューティクルそのものの量を逐次測定する方法はなかった。特に毛髪に関して、日常的な外的刺激であって比較的強い外的刺激のブラッシングから、穏やかな外的刺激である風による毛髪同士の擦れ等の幅広い外的刺激によって剥離するキューティクルそのものの量を逐次測定する方法はなかった。また、レーザー光による微粒子の可視化方法および装置については知られているが、キューティクル剥離量の測定に応用する方法は提案されていない。 However, there has been no method for sequentially measuring the amount of the cuticle itself peeled off by an external stimulus. Especially for hair, the amount of the cuticle itself that is peeled by a wide range of external stimuli, such as daily external stimuli and brushing of relatively strong external stimuli, and the gentle external stimulus such as rubbing between the hairs. There was no method of sequential measurement. Further, although a method and an apparatus for visualizing fine particles using a laser beam are known, a method applied to measurement of the amount of cuticle peeling has not been proposed.
本発明の目的は、毛髪等のキューティクルを表面に有する繊維状物質から、ブラッシング等の外的刺激によって、その都度剥離するキューティクルの量を測定する方法、及び該方法を用いて毛髪の損傷度を評価する方法を見出そうとするものである。 An object of the present invention is to measure the amount of cuticle that peels off from a fibrous material having a cuticle such as hair on the surface by an external stimulus such as brushing, and to measure the degree of damage to hair using the method. It seeks to find a way to evaluate.
本発明者は、上記課題を解決するために鋭意検討した結果、クリーンルーム内において、剥離したキューティクルを微粒子可視化システムによって光学的に捕捉することにより、上記目的を達成できることを見出し、本発明を完成するに至った。 As a result of intensive studies to solve the above problems, the present inventor has found that the above object can be achieved by optically capturing the peeled cuticle by a fine particle visualization system in a clean room, and completes the present invention. It came to.
すなわち、本発明の第１の態様であるキューティクルの剥離量の測定方法は、クリーンルーム内において、外的刺激により前記被測定物質より剥離するキューティクルを微粒子可視化システムによって光学的に捕捉し、前記キューティクルの剥離量を計測する工程と、を含むことを特徴とするものである。 That is, the cuticle peeling amount measuring method according to the first aspect of the present invention is a method of optically capturing a cuticle peeled off from the substance to be measured by an external stimulus in a clean room using a particle visualization system. And a step of measuring a peeling amount.
更に、前記態様において、前記微粒子可視化システムは、レーザーシートを生成する光源システム、前記キューティクルがレーザーシートを通過するときに発生する散乱光を捉える撮像システム、及び前記キューティクルの画像の表示又は処理を行う画像処理システムからなることが好ましい。 Further, in the above aspect, the fine particle visualization system performs display or processing of a light source system that generates a laser sheet, an imaging system that captures scattered light generated when the cuticle passes through the laser sheet, and an image of the cuticle. It preferably comprises an image processing system.
本発明の第２の態様である外的刺激による毛髪の損傷度の評価方法は、前記態様のキューティクルの剥離量の測定方法を用いて毛髪からのキューティクルの剥離量を測定し、毛
髪の損傷度を評価することを特徴とするものである。
The method for evaluating the degree of hair damage due to external stimulation according to the second aspect of the present invention comprises measuring the amount of cuticle peeling from the hair using the method for measuring the amount of peeling of the cuticle according to the aspect described above, and determining the degree of hair damage. It is characterized by evaluating.
本発明によれば、外的刺激によって剥離するキューティクルそのものの量を逐次測定することができる。 According to the present invention, it is possible to sequentially measure the amount of the cuticle itself that is peeled off by an external stimulus.
特に毛髪に関して、日常的な外的刺激であって比較的強い外的刺激のブラッシングから穏やかな外的刺激である風による毛髪同士の擦れ等の幅広い外的刺激によって剥離するキューティクルそのものの量を逐次測定することが可能になる。また、外的刺激によって剥離するキューティクルの量を測定することにより、毛髪の損傷度を評価することが可能になる。 For hair in particular, the amount of cuticles that are peeled off by a wide range of external stimuli, such as daily external stimuli and brushing of relatively strong external stimuli, and gentle external stimuli such as rubbing between the hairs, is sequentially determined. It becomes possible to measure. Moreover, it becomes possible to evaluate the damage degree of hair by measuring the amount of the cuticle peeled off by an external stimulus.
以下、本発明を詳細に説明する。 Hereinafter, the present invention will be described in detail.
本発明のキューティクル剥離量の測定方法は、外的刺激により被測定物質から剥離するキューティクルを光学的に捕捉し、キューティクルの剥離量を測定する方法である。 The method for measuring the amount of peeling of a cuticle according to the present invention is a method for optically capturing a cuticle that is peeled off from a substance to be measured by an external stimulus and measuring the amount of peeling of the cuticle.
本発明のキューティクル剥離量の測定方法は、被測定物質に外的刺激を与える工程と、外的刺激により被測定物質から剥離するキューティクルを微粒子可視化システムによって光学的に捕捉し、キューティクルの剥離量を計測する工程を含む。 The method for measuring the amount of peeling of a cuticle according to the present invention includes a step of applying an external stimulus to a substance to be measured, and optically capturing the cuticle peeled off from the substance to be measured by the external stimulus by a fine particle visualization system. Including the step of measuring.
本発明に用いられる微粒子可視化システムは、シート状のレーザー光であるレーザーシートを生成する光源部、前記キューティクルがレーザーシートを通過するときに発生する散乱光を光学的に捉える撮像部、及び前記キューティクルの画像の表示又は処理を行う画像処理部からなることが好ましい。 The particle visualization system used in the present invention includes a light source unit that generates a laser sheet that is a sheet-like laser beam, an imaging unit that optically captures scattered light generated when the cuticle passes through the laser sheet, and the cuticle. Preferably, the image processing unit is configured to display or process the image.
レーザーシートを生成する光源部は、レーザーシートを照射する光源ヘッドとレーザー光の出力を調整する光源コントローラを備える。撮像部は、被測定物質から剥離したキューティクルがレーザーシートを通過する際に発生する散乱光を捉えるための高感度カメラを備える。画像処理部は、撮像部から送られてきた画像の表示、録画及び背景減算等の画像処理を行うもので、画像中の任意の領域に映し出されたキューティクルの量を計測する機能を有していることが好ましい。このような微粒子可視化システムとしては、新日本空調株式会社が提供する微粒子可視化システム等が挙げられる。 The light source unit that generates the laser sheet includes a light source head that irradiates the laser sheet and a light source controller that adjusts the output of the laser light. The imaging unit includes a high-sensitivity camera for capturing scattered light generated when the cuticle peeled from the substance to be measured passes through the laser sheet. The image processing unit performs image processing such as display, recording, and background subtraction of the image sent from the imaging unit, and has a function of measuring the amount of the cuticle projected in an arbitrary area in the image. Preferably it is. Examples of such a particle visualization system include a particle visualization system provided by Shin Nippon Air Conditioning Co., Ltd.
本発明に用いられるレーザーシートの生成状態は特に限定されるものではないが、水平ないし略水平状であることが好ましい。水平ないし略水平状のレーザーシートとすることで、被測定物質から剥離したキューティクルの鉛直方向への自由落下を利用して、レーザーシートを通過するときに発生する散乱光を捉えることができ、測定に使用する装置を複雑な構成にする必要がない。 The production state of the laser sheet used in the present invention is not particularly limited, but is preferably horizontal or substantially horizontal. By using a horizontal or substantially horizontal laser sheet, it is possible to capture the scattered light generated when passing through the laser sheet using free fall in the vertical direction of the cuticle peeled from the substance to be measured. It is not necessary to make the apparatus used for the complicated configuration.
本発明に用いられる被測定物質は、キューティクルを表面に有する繊維状物質であり、具体的にはケラチン繊維である毛髪、羊毛等が挙げられる。測定には被測定物質を毛束状にすることが望ましい。毛束の量としては発生する散乱光の量を勘案し適宜決めることができるが、１〜２０ｇであることが望ましい。１ｇ未満ではキューティクル剥離量が少なく測定に十分な散乱光が得られないか、得ようとしてレーザー光の出力を上げることによ
る経済的な負荷が高まる。また、クリーンルーム清浄度を高めることによる経済的な負荷が高まる。２０ｇを超えると被測定物質に対して一度に均一な外的刺激を与えにくく、キューティクル剥離量が多くクリーンルームの内の清浄度に悪影響を与える可能性が増すことから好ましくなく、被測定物質の調製も困難になる。被測定物質の設置の位置と方法は特に限定されないが、レーザーシートの上部に吊り下げて設置することが好ましい。
The substance to be measured used in the present invention is a fibrous substance having a cuticle on its surface, and specifically includes keratin fibers such as hair and wool. For the measurement, it is desirable to make the substance to be measured into a hair bundle. The amount of hair bundle can be appropriately determined in consideration of the amount of scattered light generated, but is preferably 1 to 20 g. If it is less than 1 g, the amount of cuticle peeling is small, and sufficient scattered light for measurement cannot be obtained, or the economic load by increasing the output of laser light to increase it is increased. Moreover, the economic load by raising the clean room cleanliness increases. If it exceeds 20 g, it is difficult to apply a uniform external stimulus to the substance to be measured at a time, and the amount of cuticle peeling is large, which increases the possibility of adversely affecting the cleanliness of the clean room. Also becomes difficult. The position and method of installing the substance to be measured are not particularly limited, but it is preferable to suspend the substance to be measured from the upper part of the laser sheet.
本発明に用いられる被測定物質については、測定前に被測定物質表面の付着物を洗浄剤組成物等で除去しておくことが望ましい。剥離キューティクル以外の物質による散乱光を発生させないために望ましい前処理方法となる。 For the substance to be measured used in the present invention, it is desirable to remove the deposits on the surface of the substance to be measured with a cleaning composition before measurement. This is a desirable pretreatment method in order to prevent the generation of scattered light caused by substances other than the peeling cuticle.
本発明の外的刺激としては、櫛通し、手櫛通し、振動等があるが、毛髪の手入れを想定すれば櫛通しによる外的刺激が再現性の点から望ましい。手櫛通しでは手からの発塵による妨害があり好ましくない場合がある。測定前に櫛表面の付着物を洗浄剤組成物等で除去しておくことが望ましい。剥離キューティクル以外の物質による散乱光を発生させないために望ましい前処理方法となる。また、振動の例示としては、風による毛髪同士の擦れが日常的に起こり得る外的刺激として好ましい。更に、毛束の毛流れ方向に対し並行、直行または一定の角度をもって一定の振幅、速度で束ねた部位を振動させる外的刺激も日常的に起こり得るものであり好ましい例である。 Examples of the external stimulus of the present invention include combing, hand combing, vibration, and the like, and external stimulation by combing is desirable from the viewpoint of reproducibility, assuming that the hair is cared for. Hand combing may be unfavorable due to interference from hand dusting. It is desirable to remove deposits on the comb surface with a cleaning composition before measurement. This is a desirable pretreatment method in order to prevent the generation of scattered light caused by substances other than the peeling cuticle. Further, as an example of vibration, it is preferable as an external stimulus in which rubbing between hairs caused by wind can occur on a daily basis. Furthermore, an external stimulus that vibrates a part bundled at a constant amplitude and speed at a constant angle, parallel or at a constant angle with respect to the hair flow direction of the hair bundle is also a preferable example.
（キューティクルの剥離量の測定方法）
以下、本発明の一実施形態であるキューティクルの剥離量の測定方法を説明する。図１は、本方法を説明するための模式図である。本実施形態では、上記したように必要に応じて前処理を行った被測定物質である毛束９と、微粒子可視化システム１とをクリーンルーム内に設置し、光源部２の光源ヘッド３よりレーザーシート５を水平状に生成させる。次に、毛束９を水平状のレーザーシート５の上部に吊り下げて設置し、被測定物質に櫛１０を用いて櫛通しによる外的刺激を与え、毛束９から剥離したキューティクル８がレーザーシート５を通過するときに発生する散乱光を撮像部６で捉え、画像処理部７においてキューティクルの画像の表示又は処理を行い、キューティクルの剥離量を測定する。
(Method for measuring the amount of cuticle peeling)
Hereinafter, a method for measuring the amount of peeling of a cuticle according to an embodiment of the present invention will be described. FIG. 1 is a schematic diagram for explaining this method. In the present embodiment, as described above, the hair bundle 9 that is the measurement target material that has been pretreated as necessary and the fine particle visualization system 1 are installed in a clean room, and the laser sheet is formed from the light source head 3 of the light source unit 2. 5 is generated horizontally. Next, the hair bundle 9 is suspended from the upper part of the horizontal laser sheet 5, and the cuticle 8 peeled off from the hair bundle 9 is laser-exposed by applying an external stimulus to the substance to be measured using the comb 10. Scattered light generated when passing through the sheet 5 is captured by the imaging unit 6, and the image of the cuticle is displayed or processed in the image processing unit 7 to measure the amount of peeling of the cuticle.
（毛髪の損傷度の評価方法）
本発明の別の実施形態である毛髪の損傷度の評価方法では、毛髪について前記のキューティクルの剥離量の測定方法による測定を行い、測定されたキューティクルの剥離量に基づいて、毛髪の損傷度を評価する。このキューティクルの剥離量の数値が大きいものほど、より損傷度が高いと判断することができる。
(Evaluation method of hair damage)
In the method for evaluating the degree of damage to hair according to another embodiment of the present invention, the hair is measured by the method for measuring the amount of peeling of the cuticle, and the degree of damage to the hair is determined based on the measured amount of peeling of the cuticle. evaluate. It can be determined that the larger the value of the cuticle peeling amount, the higher the degree of damage.
以下に、実施例により本発明をより詳細に説明するが、本発明はこれによってなんら限定されるものではない。 Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited thereto.
健康な毛髪の毛束（２０ｃｍ、５ｇ）を８質量％ラウレス硫酸ナトリウム水溶液で洗浄後、市販のブリーチ剤で３回処理した後に自然乾燥させたダメージ毛髪と、健康な毛髪の毛束（２０ｃｍ、５ｇ）を８質量％ラウレス硫酸ナトリウム水溶液で洗浄後に自然乾燥させた健康毛髪を調製し、新日本空調株式会社が提供する微粒子可視化システムによる測定に供した。 A healthy hair bundle (20 cm, 5 g) was washed with an 8% by weight aqueous sodium laureth sulfate solution, treated with a commercially available bleaching agent three times, and then naturally dried, and a healthy hair bundle (20 cm, 5 g). Was washed with an 8% by mass aqueous sodium laureth sulfate solution and then naturally dried hair was prepared and subjected to measurement using a fine particle visualization system provided by Shin Nippon Air Conditioning Co., Ltd.
クラス１００のクリーンルーム内に水平状のレーザーシートを発生させ、その上部に前記ダメージ毛髪と健康毛髪を吊り下げ、前記ダメージ毛髪及び健康毛髪にそれぞれ市販の櫛（アルコール洗浄済み）を通して外的刺激を与えた。櫛通しは２回行い、剥離するキューティクルが落下し前記水平状のレーザーシートを通過するときに発生する散乱光を光学的に捕捉し、その画像処理によって散乱光の発生回数をカウントしキューティクル剥離量
とした。結果を表１に示す。
A horizontal laser sheet is generated in a Class 100 clean room, the damaged hair and healthy hair are suspended on the top, and external damage is applied to the damaged hair and healthy hair through commercially available combs (alcohol-washed). It was. Perform combing twice, optically capture the scattered light generated when the cuticle to be peeled falls and pass through the horizontal laser sheet, and count the number of times the scattered light is generated by the image processing, and the amount of cuticle peeling It was. The results are shown in Table 1.
表１から、健康毛髪とダメージ毛髪の各々において櫛通しによって剥離したキューティクル量の違いを評価することが可能なキューティクル剥離量の測定方法であることが明らかとなった。 From Table 1, it became clear that this is a method for measuring the amount of cuticle peel that can evaluate the difference in the amount of cuticle peeled by combing between healthy hair and damaged hair.
また、ブリーチ毛髪の毛束（２０ｃｍ、５ｇ）を吊り下げ櫛通しを行わない状態でのカウント数（計測時間は上記櫛通し２回に要した時間）は６であった。櫛通しを行わない状態であっても上から下への気流が存在するため被測定物質間の擦れが起こり、キューティクルの剥離が発生し、測定することが可能であった。ブリーチ毛髪の毛束（２０ｃｍ、２ｇ）で同様に行った測定ではカウント数（計測時間は上記櫛通し２回に要した時間）は１となり、毛髪量の違いによるキューティクル剥離量の差も明らかにできた。 Moreover, the count number (measurement time is the time required for the above two times of combing) in a state where the hair bundle (20 cm, 5 g) of bleached hair was suspended and no combing was performed was 6. Even in the state where no combing is performed, since there is an air flow from the top to the bottom, rubbing between the materials to be measured occurs, and the cuticle is peeled off. In the same measurement with a hair bundle of bleached hair (20 cm, 2 g), the count number (measurement time is the time required for the two times of combing) is 1, and the difference in the amount of cuticle peeling due to the difference in the amount of hair can also be clarified. It was.
ブリーチ毛髪の毛束（２０ｃｍ、５ｇ）を吊り下げ、その下部に表面に両面テープを貼ったプレパラートを配置し、上記と同様に櫛通しを２回行い、落下してくる物質を前記プレパラートに捕捉し、レーザー顕微鏡にて観察した結果、主に短径、長径が各々１〜３μｍ、２〜５μｍの剥離キューティクルが観察された。 A hair bundle (20 cm, 5 g) of bleached hair is suspended, and a preparation with a double-sided tape attached to the surface is placed underneath it. As a result of observation with a laser microscope, peeled cuticles having a minor axis and a major axis of 1 to 3 μm and 2 to 5 μm, respectively, were observed.
１ 微粒子可視化システム
２ 光源部
３ 光源ヘッド
４ 光源コントローラ
５ レーザーシート
６ 撮像部
７ 画像処理部
８ キューティクル
９ 毛束
１０ 櫛
DESCRIPTION OF SYMBOLS 1 Fine particle visualization system 2 Light source part 3 Light source head 4 Light source controller 5 Laser sheet 6 Imaging part 7 Image processing part 8 Cuticle 9 Hair bundle 10 Comb
Claims (3)
被測定物質に外的刺激を与える工程と、
外的刺激により前記被測定物質より剥離するキューティクルを微粒子可視化システムによって光学的に捕捉し、前記キューティクルの剥離量を計測する工程と、
を含むことを特徴とするキューティクルの剥離量の測定方法。 In a clean room,
Applying an external stimulus to the substance to be measured;
A step of optically capturing a cuticle peeled off from the substance to be measured by an external stimulus by a fine particle visualization system, and measuring a peeling amount of the cuticle;
A method for measuring the amount of delamination of a cuticle, comprising:
The degree of hair damage due to external stimuli, characterized by measuring the amount of cuticle peel from hair using the method for measuring the amount of peel of a cuticle according to claim 1 or 2, and evaluating the degree of hair damage Evaluation method.
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