JP6026432B2 - 検体濃縮容器とそれを用いた検体濃縮方法 - Google Patents
検体濃縮容器とそれを用いた検体濃縮方法 Download PDFInfo
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
- B01L3/50215—Test tubes specially adapted for centrifugation purposes using a float to separate phases
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
- B01L2300/0851—Bottom walls
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
- B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0605—Valves, specific forms thereof check valves
- B01L2400/0616—Ball valves
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4083—Concentrating samples by other techniques involving separation of suspended solids sedimentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
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Description
図1に示すごとく、本実施形態の検体濃縮容器1は、例えば、生体試料などの検体と抽出液とからなる混合液を収容し、その内部でこの混合液の濃縮が行われる容器である。検体濃縮容器1は、上面開口部(第1開口部)3を有した容器本体2と、この容器本体2の上面開口部3に開閉自在に設けた蓋4とを備えている。図1は本発明の実施形態に係る検体濃縮容器1の要部構成を示す断面図である。
なお、本実施の形態に係る検体濃縮容器1の容器本体2は、上述したようにテーパー形状部分に続いて、有底筒状の検体濃縮部6が形成される構成であった。しかしながら、検体蓋8による検体濃縮部6の上面開口部7の密閉度を高めるために、上面開口部7近傍のテーパー形状部分が、図6に示すように検体蓋8外形に沿うように弧形状に凹んだ形状となっていてもよい。図6は、本発明の実施形態の変形例に係る検体濃縮容器1の断面図である。図6では、検体濃縮部6の上面開口部7近傍における本体容器2の断面形状を示している。
2 容器本体
3 上面開口部
4 蓋
5 内部底面部
6 検体濃縮部
7 上面開口部
8 検体蓋
9 混合液
10 遠心回転装置
11 突出部
12 検体蓋本体
Claims (12)
- 減圧下において遠心機によって回転させることで、その内部で検体を含む混合液の濃縮が行われる検体濃縮容器であって、
上面に第1開口部を有する管形状の容器本体と、
前記容器本体内と連通するための第2開口部を有し、該容器本体の底部側に形成された、濃縮後の前記混合液を収容する検体濃縮部と、
前記容器本体内に配置された前記第2開口部を覆うための検体蓋と、
を備え、
前記第2開口部の内径は、前記容器本体の内径よりも小さくなっており、
前記検体濃縮部は、上部に前記第2開口部が形成された有底筒状であって、
前記検体蓋は、前記第2開口部の直径よりも大きな直径を有する球状であるとともに、前記混合液上に浮く物質によって構成されている、
検体濃縮容器。 - 前記検体蓋は、前記混合液の濃縮時において、該混合液の液面が前記検体濃縮部の前記第2開口部よりも低下したとき、該第2開口部を覆う、
請求項1に記載の検体濃縮容器。 - 減圧下において遠心機によって回転させることで、その内部で検体を含む混合液の濃縮が行われる検体濃縮容器であって、
上面が開口した容器本体と、
この容器本体の上面開口部に開閉自在に設けた蓋と、
を備え、
前記容器本体は、その内部底面部に、上面が開口し、その内径が、前記容器本体の内径よりも小さなコップ状の検体濃縮部を設けるとともに、
この検体濃縮部の上面開口部上に、この検体濃縮部の上面開口部の直径よりも大きな直径を有する球状の検体蓋を可動自在に配置した、
検体濃縮容器。 - 前記検体蓋が、合成樹脂により構成される、
請求項1〜3のいずれか一つに記載の検体濃縮容器。 - 前記検体蓋が、ポリプロピレン樹脂により構成される、
請求項1〜4のいずれか一つに記載の検体濃縮容器。 - 前記検体蓋が、ポリエチレン樹脂により構成される、
請求項1〜5のいずれか一つに記載の検体濃縮容器。 - 前記検体蓋は、前記混合液の濃縮時において、該混合液の液面が前記検体濃縮部の前記第2開口部よりも低下したとき、該第2開口部近傍で前記容器本体と面接触して、この第2開口部を覆う、
請求項2に記載の検体濃縮容器。 - 前記検体蓋は、その内部に中空部分を含む、
請求項7に記載の検体濃縮容器。 - 前記検体蓋は、球状の検体蓋本体から一方向に突出した棒形状の突出部を有しており、前記検体蓋本体の球面が下向きに、前記突出部が上向きになるように配置される、
請求項7に記載の検体濃縮容器。 - 前記突出部を含めた検体蓋の全長が、容器本体の内径よりも長くなる、
請求項9に記載の検体濃縮容器。 - 前記検体蓋は、前記第2開口部を塞ぐ面を含むとともに、前記突出部が形成されている検体蓋本体を有し、
少なくとも前記突出部の内部が中空となる、
請求項9に記載の検体濃縮容器。 - 請求項1〜11のいずれか一つに記載の検体濃縮容器を用いた検体濃縮方法であって、
前記検体濃縮容器内に、抽出液と検体との混合液を配置し、
減圧下において、前記検体濃縮容器を遠心機により回転させ、遠心力を発生させて混合液の濃縮を行う、
検体濃縮方法。
Applications Claiming Priority (3)
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JP2012003722 | 2012-01-12 | ||
JP2012003722 | 2012-01-12 | ||
PCT/JP2012/008391 WO2013105204A1 (ja) | 2012-01-12 | 2012-12-27 | 検体濃縮容器とそれを用いた検体濃縮方法 |
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JPWO2013105204A1 JPWO2013105204A1 (ja) | 2015-05-11 |
JP6026432B2 true JP6026432B2 (ja) | 2016-11-16 |
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US (1) | US9700885B2 (ja) |
EP (1) | EP2803396B1 (ja) |
JP (1) | JP6026432B2 (ja) |
CN (1) | CN104039407B (ja) |
WO (1) | WO2013105204A1 (ja) |
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US10639628B2 (en) | 2013-12-20 | 2020-05-05 | 3M Innovative Properties Company | Systems and methods for sample concentration and detection |
WO2017180749A1 (en) | 2016-04-15 | 2017-10-19 | Moorhead Mari Kilroy | Well plate |
US20210190803A1 (en) * | 2017-11-17 | 2021-06-24 | Hombrechtikon Systems Engineering Ag | Device and method for reversibly immobilising biomolecules |
CN110057899B (zh) * | 2019-04-14 | 2022-01-28 | 北京工业大学 | 厌氧氨氧化污泥胞外聚合物分子结构表征的***及方法 |
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US4214993A (en) | 1978-04-03 | 1980-07-29 | E. I. Du Pont De Nemours And Company | Apparatus for separating fluids |
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US5269927A (en) * | 1991-05-29 | 1993-12-14 | Sherwood Medical Company | Separation device for use in blood collection tubes |
US5484734A (en) | 1993-03-09 | 1996-01-16 | Torc Seimitsu Industries, Ltd. | Reaction vessel for preventing evaporation and a method thereof |
US5389265A (en) * | 1993-06-02 | 1995-02-14 | E. I. Du Pont De Nemours And Company | Phase-separation tube |
US5785925A (en) | 1996-08-29 | 1998-07-28 | Saigene Corporation | Centrifuge tube phase separation plug |
US6824738B1 (en) | 2000-04-14 | 2004-11-30 | Discovery Partners International, Inc. | System and method for treatment of samples on solid supports |
US6933109B2 (en) | 2000-12-22 | 2005-08-23 | Large Scale Proteomics Corporation | Rapid particle detection |
JP3718459B2 (ja) * | 2001-07-09 | 2005-11-24 | 株式会社荏原製作所 | 分析試料液の濃縮法及び装置 |
JP4077649B2 (ja) * | 2002-04-12 | 2008-04-16 | ユースエンジニアリング株式会社 | 溶液濃縮装置 |
US20030205538A1 (en) * | 2002-05-03 | 2003-11-06 | Randel Dorian | Methods and apparatus for isolating platelets from blood |
JP2004028819A (ja) * | 2002-06-26 | 2004-01-29 | Hiyoshi:Kk | 微量成分濃縮装置及び濃縮方法 |
US7074577B2 (en) * | 2002-10-03 | 2006-07-11 | Battelle Memorial Institute | Buffy coat tube and float system and method |
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BRPI0918358A2 (pt) | 2008-12-19 | 2015-12-15 | 3M Innovative Properties Co | sistema e método para a concentração de amostras |
EP2553114A1 (en) * | 2010-03-30 | 2013-02-06 | Battelle Memorial Institute | Buffy coat separator float systems and methods |
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- 2012-12-27 WO PCT/JP2012/008391 patent/WO2013105204A1/ja active Application Filing
- 2012-12-27 US US14/371,714 patent/US9700885B2/en active Active
- 2012-12-27 EP EP12865157.7A patent/EP2803396B1/en active Active
- 2012-12-27 CN CN201280066857.2A patent/CN104039407B/zh active Active
- 2012-12-27 JP JP2013553113A patent/JP6026432B2/ja active Active
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US9700885B2 (en) | 2017-07-11 |
JPWO2013105204A1 (ja) | 2015-05-11 |
US20150011012A1 (en) | 2015-01-08 |
EP2803396B1 (en) | 2018-02-28 |
CN104039407A (zh) | 2014-09-10 |
EP2803396A1 (en) | 2014-11-19 |
WO2013105204A1 (ja) | 2013-07-18 |
CN104039407B (zh) | 2016-07-06 |
EP2803396A4 (en) | 2015-06-17 |
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