JP5990089B2 - Composition comprising salted fermented ginseng extract - Google Patents
Composition comprising salted fermented ginseng extract Download PDFInfo
- Publication number
- JP5990089B2 JP5990089B2 JP2012247050A JP2012247050A JP5990089B2 JP 5990089 B2 JP5990089 B2 JP 5990089B2 JP 2012247050 A JP2012247050 A JP 2012247050A JP 2012247050 A JP2012247050 A JP 2012247050A JP 5990089 B2 JP5990089 B2 JP 5990089B2
- Authority
- JP
- Japan
- Prior art keywords
- salt
- composition
- salted
- ginseng extract
- present
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000203 mixture Substances 0.000 title claims description 42
- 235000020710 ginseng extract Nutrition 0.000 title claims description 25
- 235000002639 sodium chloride Nutrition 0.000 claims description 49
- 150000003839 salts Chemical class 0.000 claims description 44
- 238000000034 method Methods 0.000 claims description 17
- 238000004519 manufacturing process Methods 0.000 claims description 16
- 235000008434 ginseng Nutrition 0.000 claims description 15
- 240000004371 Panax ginseng Species 0.000 claims description 12
- 206010052428 Wound Diseases 0.000 claims description 12
- 208000027418 Wounds and injury Diseases 0.000 claims description 12
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 11
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 11
- 230000036560 skin regeneration Effects 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000004480 active ingredient Substances 0.000 claims description 10
- 238000000855 fermentation Methods 0.000 claims description 10
- 230000004151 fermentation Effects 0.000 claims description 10
- 229940026314 red yeast rice Drugs 0.000 claims description 10
- 239000002537 cosmetic Substances 0.000 claims description 9
- 235000002789 Panax ginseng Nutrition 0.000 claims description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 230000032683 aging Effects 0.000 claims description 3
- 230000035876 healing Effects 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 238000010025 steaming Methods 0.000 claims description 2
- 241000208340 Araliaceae Species 0.000 claims 3
- 230000000694 effects Effects 0.000 description 20
- 210000004027 cell Anatomy 0.000 description 13
- 239000000284 extract Substances 0.000 description 11
- 210000003491 skin Anatomy 0.000 description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 239000002552 dosage form Substances 0.000 description 8
- 230000008929 regeneration Effects 0.000 description 8
- 238000011069 regeneration method Methods 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 8
- 231100000241 scar Toxicity 0.000 description 6
- 230000037307 sensitive skin Effects 0.000 description 6
- 230000029663 wound healing Effects 0.000 description 6
- 230000003013 cytotoxicity Effects 0.000 description 5
- 231100000135 cytotoxicity Toxicity 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 239000012071 phase Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 208000032544 Cicatrix Diseases 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 239000008187 granular material Substances 0.000 description 4
- 239000003960 organic solvent Substances 0.000 description 4
- 239000008194 pharmaceutical composition Substances 0.000 description 4
- 230000037387 scars Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 244000273928 Zingiber officinale Species 0.000 description 3
- 235000006886 Zingiber officinale Nutrition 0.000 description 3
- 239000006071 cream Substances 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 235000008397 ginger Nutrition 0.000 description 3
- 230000002757 inflammatory effect Effects 0.000 description 3
- 239000006210 lotion Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 235000019198 oils Nutrition 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000001172 regenerating effect Effects 0.000 description 3
- 239000013535 sea water Substances 0.000 description 3
- 239000007901 soft capsule Substances 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 description 2
- 235000021109 kimchi Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
- 231100001083 no cytotoxicity Toxicity 0.000 description 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- -1 salt salt Chemical class 0.000 description 2
- 238000009938 salting Methods 0.000 description 2
- 235000020712 soy bean extract Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 1
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241000416162 Astragalus gummifer Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 102000016359 Fibronectins Human genes 0.000 description 1
- 108010067306 Fibronectins Proteins 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 208000033830 Hot Flashes Diseases 0.000 description 1
- 206010060800 Hot flush Diseases 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 244000294411 Mirabilis expansa Species 0.000 description 1
- 235000015429 Mirabilis expansa Nutrition 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 1
- 235000019482 Palm oil Nutrition 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 229920001615 Tragacanth Polymers 0.000 description 1
- 240000001417 Vigna umbellata Species 0.000 description 1
- 235000011453 Vigna umbellata Nutrition 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- SNAAJJQQZSMGQD-UHFFFAOYSA-N aluminum magnesium Chemical compound [Mg].[Al] SNAAJJQQZSMGQD-UHFFFAOYSA-N 0.000 description 1
- 230000001166 anti-perspirative effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 239000003213 antiperspirant Substances 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 229940069765 bean extract Drugs 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 239000002826 coolant Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000006196 drop Substances 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000003974 emollient agent Substances 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 210000005081 epithelial layer Anatomy 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 239000007902 hard capsule Substances 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 230000002390 hyperplastic effect Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 210000004969 inflammatory cell Anatomy 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000001023 inorganic pigment Substances 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 235000019359 magnesium stearate Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 235000010981 methylcellulose Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 235000013536 miso Nutrition 0.000 description 1
- 239000012046 mixed solvent Substances 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 239000012860 organic pigment Substances 0.000 description 1
- 239000003002 pH adjusting agent Substances 0.000 description 1
- 239000002540 palm oil Substances 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 238000005554 pickling Methods 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920006316 polyvinylpyrrolidine Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000035752 proliferative phase Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 1
- 230000008521 reorganization Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 235000019600 saltiness Nutrition 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 210000004761 scalp Anatomy 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 230000036555 skin type Effects 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- JUJBNYBVVQSIOU-UHFFFAOYSA-M sodium;4-[2-(4-iodophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate Chemical compound [Na+].C1=CC([N+](=O)[O-])=CC=C1N1[N+](C=2C=CC(I)=CC=2)=NC(C=2C(=CC(=CC=2)S([O-])(=O)=O)S([O-])(=O)=O)=N1 JUJBNYBVVQSIOU-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 235000021404 traditional food Nutrition 0.000 description 1
- 239000000196 tragacanth Substances 0.000 description 1
- 235000010487 tragacanth Nutrition 0.000 description 1
- 229940116362 tragacanth Drugs 0.000 description 1
- 239000006097 ultraviolet radiation absorber Substances 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 230000036642 wellbeing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Mycology (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Biotechnology (AREA)
- Public Health (AREA)
- Microbiology (AREA)
- Birds (AREA)
- Chemical & Material Sciences (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Description
本発明は、塩蔵発酵高麗人蔘抽出物を含む皮膚再生用組成物に関する。 The present invention relates to a skin regeneration composition containing a salted and fermented ginseng extract.
皮膚は、外部から身体を保護する防御膜の役割をする。このような皮膚に傷ができると、生体の自然治癒作用によりその治癒が行われる。 The skin acts as a protective film that protects the body from the outside. When such a skin is damaged, it is healed by the natural healing action of the living body.
傷の治癒過程は、大きく、炎症期と再上皮化期、増殖期、成熟期の４段階に区分される。炎症期には、血管内を移動した免疫細胞が傷の部位に出現する。続いて、顆粒組織の形成を誘導する成長因子や信号伝達物質が分泌される。重大な感染がない場合、一般的に炎症期は短く進行される。 The wound healing process is roughly divided into four stages: an inflammatory stage, a re-epithelialization stage, a proliferative stage, and a mature stage. During the inflammatory phase, immune cells that have migrated through the blood vessels appear at the site of the wound. Subsequently, growth factors and signaling substances that induce the formation of granular tissue are secreted. In the absence of a serious infection, the inflammatory phase generally proceeds short.
増殖期は、再上皮化期と類似して起こるようになるが、傷の部位において顆粒組織が形成される特性を示す。顆粒組織は、線維芽細胞（ｆｉｂｒｏｂｌａｓｔｓ）、炎症性細胞（ｉｎｆｌａｍｍａｔｏｒｙ ｃｅｌｌｓ）とともに、未成熟コラーゲン（ｉｍｍａｔｕｒｉｔｙ ｃｏｌｌａｇｅｎ）、フィブロネクチン（ｆｉｂｒｏｎｅｃｔｉｎ）又はヒアルロン酸（ｈｙａｌｕｒｏｎｉｃ ａｃｉｄ）等の細胞外基質構成要素からなっており、この顆粒組織が傷の部位を急速に埋め、組織的な構造を備えることが傷の治癒の重要な鍵となる。その後、剥がれた傷の表面が角質形成細胞（ｋｅｒａｔｉｎｏｃｙｔｅｓ）層によって覆われながら、新しい表皮が生成され、上皮層が再建される。 The proliferative phase becomes similar to the re-epithelializing phase, but exhibits the property that granular tissue is formed at the site of the wound. The granule tissue is composed of extracellular matrix components such as immature collagen, fibronectin or hyaluronic acid together with fibroblasts and inflammatory cells. It is an important key for wound healing that this granular tissue rapidly fills the wound site and has a structured structure. Thereafter, a new epidermis is generated and the epithelial layer is reconstructed while the surface of the detached wound is covered with a keratinocyte layer.
傷の再上皮化が完了すると、結合組織の増加と再編成を通じて傷の面積が減少する一連の過程が進行される。成熟期の間、回復期組織の固まった細胞と毛細血管が少しずつ消えるようになるが、このような組織が過形成され、又は正常に分解されない場合、傷痕ができることがある。 When wound re-epithelialization is complete, a series of processes is progressed in which the wound area decreases through the increase and reorganization of connective tissue. During maturation, the cells and capillaries of the convalescent tissue gradually disappear, but if such tissue is hyperplastic or does not degrade normally, scars may form.
傷は、速やかに治癒されることのみならず、副作用や傷痕なしに治癒されることが重要であるため、傷の治癒のためには、炎症を抑制し、成長因子が調節され、迅速な細胞の再生が行われることが必要である。 It is important that wounds are healed not only quickly, but without side effects and scars, so for wound healing, inflammation is suppressed, growth factors are regulated, and rapid cellular Needs to be played.
最近、ウェルビーイングを追求する傾向につれ、人工物を添加せずにそのまま発酵させて発酵物を得た後、これを活用した製品に対する要求がある。しかし、こうした天然発酵方法は、人体に有害な大腸菌、嫌気性細菌等による腐敗又は汚染の危険があるため、実際の製品生産に活用しにくい場合が多い。これを解決するために、発酵前に煮沸する等、殺菌をする方法を利用したりもするが、この場合、熱によって効能成分等が破壊され得るため、殺菌過程を含まず、かつ安全な発酵物の開発が必要である。 Recently, with the trend of pursuing well-being, there is a demand for a product utilizing the fermented product obtained by directly fermenting without adding an artificial product. However, such natural fermentation methods are often difficult to use in actual product production because of the risk of spoilage or contamination by Escherichia coli, anaerobic bacteria, and the like that are harmful to the human body. In order to solve this, a method of sterilization, such as boiling before fermentation, may be used, but in this case, since the active ingredient can be destroyed by heat, it does not include a sterilization process and is a safe fermentation Things need to be developed.
本発明は、皮膚の再生及び傷の治癒効果に優れた組成物を提供しようとするものである。また、本発明は、前記組成物を製造する方法を提供しようとするものである。 The present invention is intended to provide a composition excellent in skin regeneration and wound healing effects. The present invention is also intended to provide a method for producing the composition.
本発明の一側面は、塩蔵発酵高麗人蔘抽出物を有効成分として含む皮膚再生用組成物を提供する。
本発明の他の一側面は、高麗人蔘に塩を加え、発酵させるステップを含む皮膚再生用組成物の製造方法を提供する。
One aspect of the present invention provides a composition for skin regeneration comprising a salted and fermented ginseng extract as an active ingredient.
Another aspect of the present invention provides a method for producing a skin regenerating composition, which includes adding salt to a Korean ginger and fermenting it.
本発明に係る組成物は、塩蔵発酵高麗人蔘抽出物を有効成分として含むことにより、優れた皮膚の再生、傷の治癒及び傷痕の減少効果を有する。また、前記組成物は、細胞毒性をほとんど示さないため、敏感性皮膚を持った者も、憚ることなく使用することができる。 The composition according to the present invention has an excellent skin regeneration, wound healing, and scar reduction effect by including a salted and fermented ginseng extract as an active ingredient. Moreover, since the said composition shows little cytotoxicity, the person who has sensitive skin can use it without hesitation.
本明細書において、「皮膚」とは、動物の体表を覆う組織を意味するものであって、顔又はボディ等の体表を覆う組織だけでなく、頭皮や毛髪を含む最広義の概念である。また、本明細書において、「敏感性皮膚」は、外部刺激に対する抵抗性が低く、外部刺激によってひりつき、ほてり、灼熱感、掻痒感、紅潮、乾燥、炎症等といった異常反応が発生しやすい皮膚と定義することができ、かかる敏感性皮膚の原因としては、先天的な皮膚の特性、家族歴、病歴、化粧品等の誤用・乱用、生活環境又はストレス等を挙げることができる。本明細書において、「抽出物」は、抽出方法、抽出溶媒、抽出された成分又は抽出物の形態を問わず、天然物の成分を抜き出すことにより得られた物質をすべて含む広範囲な概念である。 In the present specification, “skin” means a tissue covering the body surface of an animal, and is the broadest concept including not only the tissue covering the body surface such as the face or body but also the scalp and hair. is there. In the present specification, “sensitive skin” refers to skin that has low resistance to external stimuli and is prone to abnormal reactions such as irritation, hot flashes, burning, itching, flushing, dryness, inflammation, etc. due to external stimuli. Examples of the cause of such sensitive skin include innate skin characteristics, family history, medical history, misuse / abuse of cosmetics, living environment or stress. In the present specification, the “extract” is a broad concept including all substances obtained by extracting natural components regardless of the extraction method, the extraction solvent, the extracted components or the form of the extract. .
以下、本発明を詳細に説明する。 Hereinafter, the present invention will be described in detail.
本発明の一側面は、塩蔵発酵高麗人蔘抽出物を有効成分として含む皮膚再生用組成物を提供する。本発明の他の一側面は、高麗人蔘に塩を加え、発酵させるステップを含む皮膚再生用組成物の製造方法を提供する。 One aspect of the present invention provides a composition for skin regeneration comprising a salted and fermented ginseng extract as an active ingredient. Another aspect of the present invention provides a method for producing a skin regenerating composition, which includes adding salt to a Korean ginger and fermenting it.
塩蔵は、塩漬とも表現される伝統的な食品貯蔵法の一つであり、動物性食品及び植物性食品ともに対して応用可能である。具体的に、塩蔵は、食品に塩を加えることにより腐敗及び汚染を防止し、正常的な発酵を助ける方法であり、主にキムチ、味噌及び塩辛類等の製造に活用されている。本発明の一側面において、塩蔵発酵は、酒漬け、キムチ、醤油及び各種塩辛の製造に利用される韓国固有の発酵方法である蔵醸を含む。 Shiozo is one of the traditional food storage methods expressed as salting, and can be applied to both animal foods and vegetable foods. Specifically, salt storage is a method of preventing normal decay and contamination by adding salt to foods and assisting normal fermentation, and is mainly used for the production of kimchi, miso, salted vegetables and the like. In one aspect of the present invention, the salted fermentation includes sake brewed rice, kimchi, soy sauce, and brewed brewed rice, which is a fermentation method unique to Korea, used for the production of various salted vegetables.
本発明の一側面において、高麗人蔘を塩で漬け、発酵させた塩蔵発酵高麗人蔘抽出物は、優れた細胞再生効能を有する。したがって、これを有効成分として含む組成物は、優れた皮膚再生効果、皮膚回復効果、さらに創傷治癒及び傷痕減少効果を有し得る。また、塩蔵発酵高麗人蔘抽出物は、細胞毒性がほとんどないため、これを含む組成物は、敏感な皮膚を持った者も自由に使用可能である。 In one aspect of the present invention, a salted and fermented ginseng extract obtained by pickling and fermenting ginseng with salt has an excellent cell regeneration effect. Therefore, the composition containing this as an active ingredient can have an excellent skin regeneration effect, skin recovery effect, and wound healing and scar reduction effect. In addition, since the salted and fermented ginseng extract has almost no cytotoxicity, the composition containing it can be used freely by those with sensitive skin.
本発明の一側面において、高麗人蔘（Ｐａｎａｘ ｇｉｎｓｅｎｇ ＣＡ Ｍａｙｅｒ）は、自然において自生する高麗人蔘である山蔘、加工していない高麗人蔘である水蔘、水蔘を洗浄し表皮を剥いで乾燥させた白蔘、又は水蔘を蒸気で蒸して加工した紅蔘をすべて含み、根、葉、実、花又は茎等、高麗人蔘のすべての部位を活用してよい。
本発明の一側面において、前記塩蔵に使用する塩は、塩化ナトリウムを主成分とする塩辛い味の結晶体を意味し、干潟で濃度を濃くした海水を煮て得る塩、具体的に、天日に乾かした干潟の砂に海水を通過させて塩度を高めた後、釜で煮沸して作る韓国伝統在来塩である煮塩、海水を塩田に引き込んだ後、風と日光で水分を蒸発させて作った天日塩、塩分を多く含んでいた湖から水が蒸発し残った塩が堆積して地層や岩石をなす塩であって採掘により生産された岩塩、原塩を溶解してＭｇ2+、Ｃａ2+といった不純物を取り除いた後、再結晶して得る精製塩（味塩）、及び天日塩を水に溶解させた後に不純物を取り除き、再び加熱して結晶化した再製塩（花塩）からなる群で選択された一つ以上を含むが、これらに限定されるものではない。本発明の他の一側面において、塩蔵に使用する塩は、０．１〜１０％（ｗ／ｖ）、具体的に１〜５％（ｗ／ｖ）濃度の塩水を含む。
In one aspect of the present invention, Panax ginseng CA Mayer is a Korean cocoon that grows naturally in nature, a water jar that is an unprocessed ginseng, and the skin is peeled off. All the parts of Korean ginseng such as roots, leaves, berries, flowers or stems may be used, including all the white pods dried in the above or steamed steamed syrup.
In one aspect of the present invention, the salt used in the salt storage means a salty-tasting crystal body mainly composed of sodium chloride, and is a salt obtained by boiling seawater concentrated in a tidal flat, specifically, sun After passing seawater through dried tidal flat sand to increase the saltiness, boiled in a kettle and boiled in the traditional Korean salt, seawater is drawn into the salt field, and then moisture is evaporated by wind and sunlight You are allowed to make the solar salt, rock salt water from the lake that contained a lot of salt produced by mining a vaporized remaining salt salt forming a strata or rock is deposited is, by dissolving a raw salt Mg 2+ From purified salt (flavored salt) obtained by recrystallization after removing impurities such as Ca 2+ , and regenerated salt (flower salt) obtained by dissolving impurities after dissolving sun salt in water and heating again to crystallize One or more selected from the group consisting of, but not limited to. In another aspect of the present invention, the salt used for salting contains 0.1-10% (w / v), specifically 1-5% (w / v) salt water.
本発明の一側面において、発酵は、食用可能な微生物、具体的にカビ、より具体的にコウジを利用して行ってよい。本発明の他の一側面において、発酵は、１８℃〜２８℃、具体的に２２℃〜２４℃で、３日〜６ヶ月、具体的に４日〜１ヶ月、より具体的に５日〜１０日間行われてよい。 In one aspect of the present invention, the fermentation may be performed using edible microorganisms, specifically mold, and more specifically koji. In another aspect of the present invention, the fermentation is performed at 18 to 28 ° C., specifically 22 to 24 ° C., 3 days to 6 months, specifically 4 days to 1 month, more specifically 5 days to It may be done for 10 days.
本発明の一側面において、塩蔵発酵高麗人蔘抽出物は、塩蔵発酵高麗人蔘を、水、有機溶媒、又は水と有機溶媒の混合溶媒で抽出して得てよい。前記において、水は、精製水を含み、有機溶媒は、アルコール、グリセリン、酢酸エチル、ブチレングリコール、プロピレングリコール、ジクロロメタン及びヘキサンからなる群から選択された一つ以上を含む。前記において、アルコールは、Ｃ1〜Ｃ5の低級アルコールを含み、Ｃ1〜Ｃ5の低級アルコールは、メタノール、エタノール、イソプロピルアルコール、ｎ‐プロピルアルコール、ｎ‐ブタノール及びイソブタノールからなる群から選択される一つ以上を含むが、これらに限定されるものではない。本発明の一側面において、抽出は、２０〜７０℃、具体的に３０〜５０℃で、４〜１２時間、具体的に５〜１０時間行われてよい。 In one aspect of the present invention, the salted and fermented ginseng extract may be obtained by extracting the salted and fermented ginseng with water, an organic solvent, or a mixed solvent of water and an organic solvent. In the above, water includes purified water, and the organic solvent includes one or more selected from the group consisting of alcohol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, and hexane. Selection In the above, the alcohol includes lower alcohols C 1 -C 5, lower alcohols C 1 -C 5 include methanol, ethanol, isopropyl alcohol, n- propyl alcohol, from the group consisting of n- butanol and isobutanol Including one or more of, but not limited to. In one aspect of the invention, the extraction may be performed at 20-70 ° C., specifically 30-50 ° C., for 4-12 hours, specifically 5-10 hours.
本発明の一側面による組成物は、組成物全体の重量を基礎に、０．０１重量％〜５０重量％、具体的に０．１重量％〜３０重量％、より具体的に１重量％〜１０重量％の塩蔵発酵高麗人蔘抽出物を含んでよい。前記範囲で含む場合、本発明の意図した効果を発現するのに適切であるだけでなく、組成物の安定性及び安全性をともに満足することができ、費用対効果の面においても、前記範囲で使用することが適切であり得る。 The composition according to one aspect of the present invention is based on the total weight of the composition, 0.01 wt% to 50 wt%, specifically 0.1 wt% to 30 wt%, more specifically 1 wt% to 10% by weight of salted and fermented ginseng extract may be included. When included in the above range, not only is it suitable for expressing the intended effect of the present invention, but also the stability and safety of the composition can be satisfied together, and in terms of cost effectiveness, the above range is also included. May be appropriate to use.
本発明の一側面による塩蔵発酵高麗人蔘抽出物を含む皮膚再生用組成物の製造方法は、高麗人蔘に塩を加え、発酵させるステップ以前に、高麗人蔘を塩で熟成させるステップ；及び、熟成された高麗人蔘を蒸熱して紅蔘を製造するステップをさらに含んでよい。前記熟成は、２２〜２４℃で、１日〜１５日間、具体的に５日〜１０日間行われてよい。 A method for producing a composition for skin regeneration comprising a salted and fermented ginseng extract according to one aspect of the present invention, the step of aging the ginseng with salt before adding the salt to the ginseng and fermenting; The method may further include the step of steaming the aged Korean ginseng to produce red koji. The aging may be performed at 22 to 24 ° C. for 1 to 15 days, specifically 5 to 10 days.
本発明の他の一側面による塩蔵発酵高麗人蔘抽出物を含む皮膚再生用組成物の製造方法は、高麗人蔘に塩を加え、発酵させるステップ以後に、発酵させた高麗人蔘を溶媒で抽出するステップ；溶媒で抽出した後、常温で冷浸、加熱及び濾過して液状物を得るステップ；及び、必要な場合、液状物の溶媒を蒸発させ、又は噴霧乾燥若しくは凍結乾燥して粉末に製造するステップをさらに含んでよい。 According to another aspect of the present invention, there is provided a method for producing a composition for skin regeneration comprising a salted and fermented ginseng extract. After the step of adding salt to the ginseng and fermenting the fermented ginseng with a solvent, An extraction step; after extraction with a solvent, a step of obtaining a liquid substance by immersion at room temperature, heating and filtration; and if necessary, evaporating the solvent of the liquid substance, or spray drying or freeze drying to form a powder. A manufacturing step may further be included.
本発明の一側面は、塩蔵発酵高麗人蔘抽出物を有効成分として含む化粧料組成物を提供する。前記化粧料組成物は、皮膚再生効果を有してよく、具体的に、傷を治癒し、傷痕を減少させてよい。特に、前記化粧料組成物は、皮膚細胞に対する毒性が低いため、使用者の皮膚のタイプを問わず使用可能である。前記皮膚のタイプは、敏感性皮膚又は乾燥皮膚を含む。 One aspect of the present invention provides a cosmetic composition comprising a salted and fermented ginseng extract as an active ingredient. The cosmetic composition may have a skin regeneration effect. Specifically, the cosmetic composition may heal wounds and reduce scars. In particular, since the cosmetic composition has low toxicity to skin cells, it can be used regardless of the type of skin of the user. The skin type includes sensitive skin or dry skin.
本発明に係る化粧料組成物は、局所適用に適したあらゆる剤形で提供されてよい。たとえば、溶液、水相に油相を分散させて得たエマルジョン、油相に水相を分散させて得たエマルジョン、懸濁液、固体、ゲル、粉末、ペースト、泡沫（ｆｏａｍ）又はエアロゾル組成物の剤形で提供されてよい。こうした剤形の組成物は、当該分野の通常的な方法に従って製造されてよい。 The cosmetic composition according to the present invention may be provided in any dosage form suitable for topical application. For example, a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an aqueous phase in an oil phase, a suspension, a solid, a gel, a powder, a paste, a foam or an aerosol composition May be provided in various dosage forms. Such dosage form compositions may be prepared according to routine methods in the art.
本発明に係る化粧料組成物は、前記した物質以外に、主効果を損なわない範囲内で、好ましくは主効果に相乗効果を与え得る他の成分を含んでよい。また、本発明に係る化粧料組成物は、保湿剤、エモリエント剤、界面活性剤、紫外線吸収剤、防腐剤、殺菌剤、酸化防止剤、ｐＨ調整剤、有機及び無機顔料、香料、冷感剤又は制汗剤をさらに含んでよい。前記成分の配合量は、本発明の目的及び効果を損なわない範囲内で当業者が容易に選定可能であり、その配合量は、組成物全体の重量を基準に０．０１〜５重量％、具体的に０．０１〜３重量％であってよい。 The cosmetic composition according to the present invention may contain, in addition to the substances described above, other components that can give a synergistic effect to the main effect, as long as the main effect is not impaired. Further, the cosmetic composition according to the present invention comprises a moisturizer, emollient, surfactant, ultraviolet absorber, preservative, bactericidal agent, antioxidant, pH adjuster, organic and inorganic pigments, fragrance, and cooling agent. Or it may further contain an antiperspirant. The blending amount of the components can be easily selected by those skilled in the art within a range not impairing the object and effect of the present invention, and the blending amount is 0.01 to 5% by weight based on the weight of the entire composition, Specifically, it may be 0.01 to 3% by weight.
本発明の一側面は、塩蔵発酵高麗人蔘抽出物を有効成分として含む薬学組成物を提供する。前記薬学組成物は、皮膚再生効果を有してよく、具体的に、傷を治癒し、傷痕を減少させてよい。 One aspect of the present invention provides a pharmaceutical composition comprising a salted and fermented ginseng extract as an active ingredient. The pharmaceutical composition may have a skin regeneration effect, specifically heal wounds and reduce scars.
本発明の一側面による薬学組成物は、経口、直腸、局所、経皮、静脈内、筋肉内、腹腔内、皮下等に投与されてよい。 The pharmaceutical composition according to one aspect of the present invention may be administered orally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously and the like.
経口投与のための剤形は、錠剤、丸剤、軟質及び硬質カプセル剤、顆粒剤、散剤、細粒剤、液剤、乳濁剤又はペレット剤であってよいが、これらに限定されるものではない。これら製剤は、有効成分以外に、希釈剤（例：ラクトース、デキストロース、スクロース、マンニトール、ソルビトール、セルロース又はグリシン）、滑沢剤（例：シリカ、タルク、ステアリン酸又はポリエチレングリコール）、又は結合剤（例：マグネシウムアルミニウムシリケート、澱粉ペースト、ゼラチン、トラガカンス、メチルセルロース、ナトリウムカルボキシメチルセルロース又はポリビニルピロリジン）を含有してよい。場合に応じて、崩解剤、吸収剤、着色剤、香味剤又は甘味剤等の薬剤学的添加剤を含有してよい。前記錠剤は、通常的な混合、顆粒化又はコーティング方法により製造されてよい。 Dosage forms for oral administration may be, but are not limited to, tablets, pills, soft and hard capsules, granules, powders, fine granules, solutions, emulsions or pellets. Absent. In addition to the active ingredient, these preparations include diluents (eg lactose, dextrose, sucrose, mannitol, sorbitol, cellulose or glycine), lubricants (eg silica, talc, stearic acid or polyethylene glycol), or binders ( Examples: magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose or polyvinylpyrrolidine). Depending on the case, you may contain pharmaceutical additives, such as a disintegrating agent, an absorber, a coloring agent, a flavoring agent, or a sweetening agent. The tablets may be manufactured by conventional mixing, granulating or coating methods.
非経口投与のための製剤は、注射剤、点滴剤、ローション、軟膏、ゲル、クリーム、懸濁剤、乳剤、坐剤、パッチ又は噴霧剤であってよいが、これらに限定されるものではない。 Formulations for parenteral administration may be, but are not limited to, injections, drops, lotions, ointments, gels, creams, suspensions, emulsions, suppositories, patches or sprays. .
本発明の一側面による薬学組成物の有効成分は、投与を受ける対象の年齢、性別、体重、病理状態及びその深刻度、投与経路又は処方者の判断に応じて変わるであろう。こうした因子に基づく適用量の決定は当業者の水準内にあり、その１日投与用量は、たとえば、０．１ｍｇ／ｋｇ／日〜１００ｍｇ／ｋｇ／日、より具体的には５ｍｇ／ｋｇ／日〜５０ｍｇ／ｋｇ／日とされてよいが、これに限定されるものではない。 The active ingredient of the pharmaceutical composition according to one aspect of the present invention will vary depending on the age, sex, weight, pathological condition and severity of the subject to be administered, the route of administration or the judgment of the prescriber. The determination of the dosage based on these factors is within the level of those skilled in the art, and the daily dosage is, for example, 0.1 mg / kg / day to 100 mg / kg / day, more specifically 5 mg / kg / day. Although it may be set to -50 mg / kg / day, it is not limited to this.
以下、実施例及び実験例を挙げつつ、本発明の構成及び効果について具体的に説明する。しかし、これらの実施例及び実験例は、本発明に対する理解を助けるために例示の目的としてのみ提供されたものであるだけで、本発明の範疇及び範囲がそれによって制限されるものではない。 Hereinafter, the configuration and effects of the present invention will be specifically described with reference to examples and experimental examples. However, these examples and experimental examples are provided for illustrative purposes only to help understanding of the present invention, and the scope and scope of the present invention are not limited thereby.
［実施例１〜４］塩蔵発酵紅蔘抽出物の製造
煮塩が高麗人蔘を適当に覆うように高麗人蔘１ｋｇ及び適当量の煮塩を陶器に入れ、７日間熟成させる。その後、簡単に洗浄した後、９８℃の高圧蒸気滅菌器で３〜２４時間蒸熱して紅蔘として製造する。さらに、６０℃の温風乾燥機で２４時間乾燥させる。その後、乾燥した紅蔘重量に対して１０重量％の麹、及び紅蔘が覆われる程度の２％塩（煮塩）水を加え、４〜５日間発酵を進行する。その後、簡単に洗浄した後、水又は有機溶媒を利用して４５ｇの塩蔵紅蔘発酵抽出物を収得し、これを実施例１とする。
[Examples 1-4] Manufacture of salted fermented red yeast rice extract 1 kg of Korean ginger and an appropriate amount of boiled salt are placed in a pottery so that the boiled salt covers the ginseng appropriately, and aged for 7 days. Then, after simple washing, steamed in a high-pressure steam sterilizer at 98 ° C. for 3 to 24 hours to produce red yeast rice. Furthermore, it is made to dry for 24 hours with a 60 degreeC warm air dryer. Thereafter, 10% by weight of strawberry and 2% salt (boiled salt) water to the extent that the red potato is covered are added to the dried red potato weight, and the fermentation proceeds for 4 to 5 days. Thereafter, after simple washing, 45 g of salted red yeast rice fermented extract was obtained using water or an organic solvent.
実施例１の製造方法と実質的に同一の方法を使用するが、煮塩の代わりに天日塩を使用して５５ｇの塩蔵紅蔘発酵抽出物を収得し、これを実施例２とする。 A method substantially the same as the production method of Example 1 is used, but 55 g of salted red yeast rice fermented extract is obtained using sun salt instead of boiled salt, which is referred to as Example 2.
実施例１の製造方法と実質的に同一の方法を使用するが、煮塩の代わりに再製塩を使用して４０ｇの塩蔵紅蔘発酵抽出物を収得し、これを実施例３とする。 Although substantially the same method as the production method of Example 1 is used, 40 g of salted red yeast rice fermented extract is obtained by using regenerated salt instead of boiled salt, and this is referred to as Example 3.
実施例１の製造方法と実質的に同一の方法を使用するが、煮塩の代わりに精製塩を使用して４０ｇの塩蔵紅蔘発酵抽出物を収得し、これを実施例４とする。 Although substantially the same method as the production method of Example 1 is used, 40 g of salted red yeast rice fermented extract is obtained using purified salt instead of boiled salt, which is referred to as Example 4.
［実施例５］細胞再生評価
塩蔵発酵高麗人蔘抽出物の細胞再生効果を評価するために、次のような実験を実施した。まず、９６ウェルプレートにＨａＣａＴ １×１０4細胞／ウェルを接種した。ＦＢＳが添加されていない培地に交換し、１時間培養した。その後、培地を除去し、ＦＢＳが１％含有された培地に、１、５、１０、５０及び１００ｐｐｍの実施例１と、各々１０、５０及び１００ｐｐｍの実施例２〜４の塩蔵発酵高麗人蔘抽出物を溶かした後、処理した。このとき、１％及び１０％のＦＢＳが含有された培地を陽性対照群とした。４８時間培養した後、ＷＳＴ‐１を１００μＬ／ウェルで処理した。その後、３７℃、５％ＣＯ2培養器で３時間培養した。培養後、マイクロプレートリーダー（ｍｉｃｒｏｐｌａｔｅ ｒｅａｄｅｒ）を利用して４５０ｎｍにおける吸光度を測定することにより、細胞再生効果を確認した。その結果を図１に示した。
[Example 5] Cell regeneration evaluation In order to evaluate the cell regeneration effect of the salted and fermented ginseng extract, the following experiment was conducted. First, a 96-well plate was inoculated with HaCaT 1 × 10 4 cells / well. The medium was replaced with a medium not added with FBS and cultured for 1 hour. Thereafter, the medium was removed, and the medium containing 1% FBS was subjected to 1, 5, 10, 50, and 100 ppm of Example 1, and 10, 50, and 100 ppm of Examples 2 to 4, respectively. The extract was dissolved and then processed. At this time, a medium containing 1% and 10% FBS was used as a positive control group. After culturing for 48 hours, WST-1 was treated with 100 μL / well. Thereafter, the cells were cultured at 37 ° C. in a 5% CO 2 incubator for 3 hours. After culturing, the cell regeneration effect was confirmed by measuring the absorbance at 450 nm using a microplate reader. The results are shown in FIG.
図１から見られるように、実施例１〜４の塩蔵発酵高麗人蔘抽出物は、いずれも細胞再生効能を示した。その中でも、煮塩を利用した塩蔵発酵高麗人蔘抽出物である実施例１が、１％ＦＢＳに対して有意かつ最も優れた細胞再生効能を示した。これに通じて、塩蔵発酵高麗人蔘抽出物、その中でも煮塩を利用した塩蔵発酵高麗人蔘抽出物は、細胞再生を促進して、皮膚の再生及び傷の治癒効果があることを知ることができる。 As can be seen from FIG. 1, the salted and fermented ginseng extracts of Examples 1 to 4 all exhibited cell regeneration effects. Among them, Example 1 which is a salted and fermented ginseng extract using boiled salt showed a significant and most excellent cell regeneration effect with respect to 1% FBS. Through this, the salted and fermented ginseng extract, especially the salted and fermented ginseng extract using boiled salt, promotes cell regeneration and has the effect of regenerating skin and healing wounds. Can do.
［実施例６］細胞毒性評価（ＭＴＴ分析）
塩蔵発酵高麗人蔘抽出物の細胞毒性を評価するために、次のような実験を実施した。まず、９６ウェルプレートにＨａＣａＴ １×１０4細胞／ウェルを接種した。２４時間培養後、ＦＢＳが入っていない培地でさらに２４時間培養した。その後、培地を除去し、１００μｌのＰＢＳを入れた後、４０ｍＪのＵＶＢを照射した。実施例１の塩蔵発酵紅蔘抽出物を、１、１０及び１００ｐｐｍで処理して４８時間培養した後、ＭＴＴ溶液（０．５ｍｇ／ｍＬ）を１００μｌずつ入れ、さらに３時間、３７℃の培養器で培養した。このとき、ＵＶＢを照射しなかった対照群もともに実験した。溶液を削除すると、形成されるホルマザンを１００μｌのＤＭＳＯに溶かした後、５７０ｎｍにおける吸光度を測定して細胞毒性を確認した。その結果を図２に示した。
[Example 6] Cytotoxicity evaluation (MTT analysis)
In order to evaluate the cytotoxicity of the salted fermented ginseng extract, the following experiment was performed. First, a 96-well plate was inoculated with HaCaT 1 × 10 4 cells / well. After culturing for 24 hours, the cells were further cultured for 24 hours in a medium not containing FBS. Thereafter, the medium was removed, 100 μl of PBS was added, and 40 mJ UVB was irradiated. After the salted and fermented red yeast rice extract of Example 1 was treated with 1, 10 and 100 ppm and cultured for 48 hours, 100 μl each of MTT solution (0.5 mg / mL) was added, and further incubated for 3 hours at 37 ° C. In culture. At this time, a control group that was not irradiated with UVB was also tested. When the solution was deleted, the formazan formed was dissolved in 100 μl of DMSO, and then the absorbance at 570 nm was measured to confirm cytotoxicity. The results are shown in FIG.
図２から見られるように、塩蔵発酵紅蔘抽出物は、細胞毒性がほとんどないため、敏感性皮膚を持つ者にも使用可能である。 As can be seen from FIG. 2, the salted and fermented red yeast rice extract has almost no cytotoxicity, so that it can also be used by persons with sensitive skin.
本発明の一側面による組成物の剤形例を以下に説明するが、他の様々な剤形にも応用可能であり、これは、本発明を限定しようとするものでなく、ただ具体的に説明しようとするためのものである。 Examples of dosage forms of compositions according to one aspect of the present invention are described below, but can be applied to various other dosage forms, which are not intended to limit the present invention. It is for trying to explain.
［剤形例１］ローション
下表に記載された組成により、通常の方法に従ってローションを製造する。
[Dosage Form Example 1] Lotion A lotion is produced according to the usual method according to the composition described in the table below.
［剤形例２］クリーム
下表に記載された組成により、通常の方法に従ってクリームを製造する。
[Dosage Form Example 2] Cream According to the usual method, a cream is produced according to the composition described in the table below.
［剤形例３］軟質カプセル剤
塩蔵発酵高麗人蔘抽出物１００ｍｇ、大豆抽出物５０ｍｇ、大豆油１８０ｍｇ、紅蔘抽出物５０ｍｇ、パーム油２ｍｇ、パーム硬化油８ｍｇ、黄蝋４ｍｇ及びレシチン６ｍｇを混合し、通常の方法に従って軟質カプセルを製造する。
[Formulation Example 3] Soft capsule 100 mg salted fermented ginseng extract, 50 mg soybean extract, 180 mg soybean oil, 50 mg red bean extract, 2 mg palm oil, 8 mg palm hardened oil, 4 mg yellow wax and 6 mg lecithin mixed Then, a soft capsule is produced according to a usual method.
［剤形例４］錠剤
塩蔵発酵高麗人蔘抽出物１００ｍｇ、大豆抽出物５０ｍｇ、ブドウ糖１００ｍｇ、紅蔘抽出物５０ｍｇ、澱粉９６ｍｇ及びマグネシウムステアレート４ｍｇを混合し、エタノールを添加して顆粒を形成した後乾燥し、錠剤に打錠する。
[Formulation Example 4] Tablets Salted fermented ginseng extract 100 mg, soybean extract 50 mg, glucose 100 mg, red yeast rice extract 50 mg, starch 96 mg and magnesium stearate 4 mg were mixed, and ethanol was added to form granules. After drying, it is compressed into tablets.
Claims (10)
高麗人蔘を塩で熟成させるステップ；及び
熟成された高麗人蔘を蒸熱して紅蔘を製造するステップをさらに含む、請求項７に記載の製造方法。 Before the fermenting step,
The method according to claim 7, further comprising: aging the ginseng with salt; and steaming the aged ginseng to produce red koji.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020110117590A KR101583558B1 (en) | 2011-11-11 | 2011-11-11 | Composition comprising salted and fermented ginseng extract |
| KR10-2011-0117590 | 2011-11-11 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2013103936A JP2013103936A (en) | 2013-05-30 |
| JP5990089B2 true JP5990089B2 (en) | 2016-09-07 |
Family
ID=48308187
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012247050A Active JP5990089B2 (en) | 2011-11-11 | 2012-11-09 | Composition comprising salted fermented ginseng extract |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JP5990089B2 (en) |
| KR (1) | KR101583558B1 (en) |
| CN (1) | CN103099773B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101525873B1 (en) * | 2015-03-18 | 2015-06-04 | (주)에스디생명공학 | Cosmetic composition containing Manufacturing method and Cultured wild Ginseng Roots, Hedysarum ussuriensis, Sophora flavescens and Adenophora triphylla by two-stage fermentation for improving skin |
| KR101809182B1 (en) | 2016-08-09 | 2017-12-14 | (주)화인메디 | Composition for wound dressing with natural extract |
| KR102577680B1 (en) * | 2020-12-21 | 2023-09-11 | 이종원 | Preparation of chlorine dioxide water with improved long-term stability, eco-friendly sterilization, disinfection and deodorant using the same, and method for manufacturing the same |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030009454A (en) * | 2000-05-31 | 2003-01-29 | 카가쿠키쥬쯔 신코지교단 | SKIN TISSUE REGENERATION PROMOTERS COMPRISING GINSENOSIDE Rb1 |
| WO2009057836A1 (en) * | 2007-10-31 | 2009-05-07 | Amorepacific Corporation | Use of melanin biosynthesis inhibitors from korean ginseng and the cosmetic composition containing thereof for skin whitening |
| KR101199209B1 (en) | 2008-08-01 | 2012-11-07 | (주)아모레퍼시픽 | Cosmetic composition containing fermented extracts with salt of natural materials |
| WO2010013885A1 (en) * | 2008-08-01 | 2010-02-04 | Amorepacific Corporation | Cosmetic composition containing salt-fermented extract of natural materials |
| KR20110087763A (en) * | 2010-01-27 | 2011-08-03 | 최향자 | SKIN REFRESHING AND MAKE-UP WATER PROCESS OF MANUFACTURE FOR WRINKLES IMPROVEMENT THAT USE GINSENG SAPONIN Rg1 |
| KR20110098123A (en) * | 2010-02-26 | 2011-09-01 | (주)아모레퍼시픽 | Composition containing ginseng berry fermented extracts with salt |
-
2011
- 2011-11-11 KR KR1020110117590A patent/KR101583558B1/en active IP Right Grant
-
2012
- 2012-11-08 CN CN201210443044.2A patent/CN103099773B/en active Active
- 2012-11-09 JP JP2012247050A patent/JP5990089B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103099773B (en) | 2017-09-22 |
| CN103099773A (en) | 2013-05-15 |
| JP2013103936A (en) | 2013-05-30 |
| KR20130052242A (en) | 2013-05-22 |
| KR101583558B1 (en) | 2016-01-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101194059B1 (en) | A cleansing composition for preventing loss of hair and promoting growth of hair comprising herbal extracts and the process of preparation thereof | |
| KR101393007B1 (en) | Cosmetic Composition for Skin-aging Protection and Wrinkle Improvement Comprising the Extract of Nephelium lappaceum and Litchi chinensis sonn as Active Ingredient | |
| KR101744103B1 (en) | Composition for preventing hair loss and promoting hair growth and mathod for preparing the same | |
| KR20180110939A (en) | Composition for preventing hair loss and promoting hair growth comprising plant extract | |
| CN110072516B (en) | Cosmetic composition comprising extracts of Chinese medicinal materials as effective ingredients | |
| KR102004322B1 (en) | Cosmetic composition containing botanical extract complex | |
| JP5990089B2 (en) | Composition comprising salted fermented ginseng extract | |
| KR101981016B1 (en) | Functional moisturizing, antioxidant and wrinkle-improving functional cosmetic composition and preparing method thereof | |
| WO2017052269A1 (en) | Hair growth promoting and anti-inflammatory composition comprising composite extract of natural products | |
| KR20210058772A (en) | Composition for treating hair loss and promoting hair growth | |
| KR102054100B1 (en) | Cosmetic composition containing fermented extract of roots | |
| KR20150105029A (en) | Morus Alba Leaf, Ramulus Mori, Mori radicis Cortex, Mulberry and Phellinus linteus mixed extract and manufacturing method thereof | |
| KR102017132B1 (en) | Cosmetic composition comprising a mixed fermentation extract of cinnamomum verum and angelica dahurica | |
| KR100981344B1 (en) | The cosmetic composition for preventing the skin aging | |
| JP4247091B2 (en) | Skin anti-aging agent | |
| KR102612017B1 (en) | Composition for skin improvement comprising multiple extract | |
| KR101888133B1 (en) | Composition For Promoting Synthesis of Collagen and Cosmetic Composition Using Extracts of Roots of Chrysanthemum indicum | |
| JP6581570B2 (en) | Composition containing smoked extract | |
| KR101581285B1 (en) | Skin anti-wrinkle cosmetic composition containing mixed medicinal herb extract | |
| KR101731794B1 (en) | Skin whitening or antiinflamatory composition containing plant extract of distylium racemosum, morus cathayana hemsl and eupatorium fortunei, and method for producing the same | |
| KR102673345B1 (en) | Composition for improving skin conditions comprising Centella Asiatica extract, Symphytum Officinale extract and Hydrangea Macrophylla extract | |
| KR102411893B1 (en) | Composition for inhibiting sebum secretion comprising Chestnut bur extract as an active ingredient | |
| KR20150052907A (en) | Cosmetic composition for Improving Skin Moisturization Comprising the mixed extract of Peach blossom, Sophora japonica flower, Rhubarb and Clove buds as active ingredient | |
| KR102541810B1 (en) | Compositions for improving skin comprising angelica and cranberry extracts and uses thereof | |
| KR20130012423A (en) | Cosmetic composition comprising extract of thin film outer cover of luffa roemer seed for skin whitening |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20151028 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20160712 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20160812 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 5990089 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |