JP5952783B2 - 抗菌剤 - Google Patents
抗菌剤 Download PDFInfo
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- JP5952783B2 JP5952783B2 JP2013134151A JP2013134151A JP5952783B2 JP 5952783 B2 JP5952783 B2 JP 5952783B2 JP 2013134151 A JP2013134151 A JP 2013134151A JP 2013134151 A JP2013134151 A JP 2013134151A JP 5952783 B2 JP5952783 B2 JP 5952783B2
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- antibacterial agent
- antibacterial
- rosemary
- clove
- magnesium oxide
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- 239000003242 anti bacterial agent Substances 0.000 title claims description 35
- 244000178231 Rosmarinus officinalis Species 0.000 claims description 23
- 235000016639 Syzygium aromaticum Nutrition 0.000 claims description 22
- 239000000395 magnesium oxide Substances 0.000 claims description 20
- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Inorganic materials [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 claims description 20
- AXZKOIWUVFPNLO-UHFFFAOYSA-N magnesium;oxygen(2-) Chemical compound [O-2].[Mg+2] AXZKOIWUVFPNLO-UHFFFAOYSA-N 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 11
- 239000000284 extract Substances 0.000 claims description 8
- 244000061408 Eugenia caryophyllata Species 0.000 claims 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 22
- 230000000844 anti-bacterial effect Effects 0.000 description 21
- 244000223014 Syzygium aromaticum Species 0.000 description 20
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 18
- 230000000052 comparative effect Effects 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 9
- 239000001569 carbon dioxide Substances 0.000 description 9
- 229910002092 carbon dioxide Inorganic materials 0.000 description 9
- DOUMFZQKYFQNTF-WUTVXBCWSA-N (R)-rosmarinic acid Chemical compound C([C@H](C(=O)O)OC(=O)\C=C\C=1C=C(O)C(O)=CC=1)C1=CC=C(O)C(O)=C1 DOUMFZQKYFQNTF-WUTVXBCWSA-N 0.000 description 8
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 description 8
- 230000007226 seed germination Effects 0.000 description 7
- 241000246358 Thymus Species 0.000 description 6
- 235000007303 Thymus vulgaris Nutrition 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 235000002020 sage Nutrition 0.000 description 6
- 239000001585 thymus vulgaris Substances 0.000 description 6
- 235000013824 polyphenols Nutrition 0.000 description 5
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 4
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 239000005770 Eugenol Substances 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 4
- 241000209094 Oryza Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 description 4
- ZZAFFYPNLYCDEP-HNNXBMFYSA-N Rosmarinsaeure Natural products OC(=O)[C@H](Cc1cccc(O)c1O)OC(=O)C=Cc2ccc(O)c(O)c2 ZZAFFYPNLYCDEP-HNNXBMFYSA-N 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
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- 229960002217 eugenol Drugs 0.000 description 4
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 4
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- 235000009566 rice Nutrition 0.000 description 4
- DOUMFZQKYFQNTF-MRXNPFEDSA-N rosemarinic acid Natural products C([C@H](C(=O)O)OC(=O)C=CC=1C=C(O)C(O)=CC=1)C1=CC=C(O)C(O)=C1 DOUMFZQKYFQNTF-MRXNPFEDSA-N 0.000 description 4
- TVHVQJFBWRLYOD-UHFFFAOYSA-N rosmarinic acid Natural products OC(=O)C(Cc1ccc(O)c(O)c1)OC(=Cc2ccc(O)c(O)c2)C=O TVHVQJFBWRLYOD-UHFFFAOYSA-N 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000004599 antimicrobial Substances 0.000 description 3
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- 230000001988 toxicity Effects 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- WMBWREPUVVBILR-WIYYLYMNSA-N (-)-Epigallocatechin-3-o-gallate Chemical compound O([C@@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=C(O)C=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-WIYYLYMNSA-N 0.000 description 2
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 description 2
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 description 2
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- WMBWREPUVVBILR-UHFFFAOYSA-N GCG Natural products C=1C(O)=C(O)C(O)=CC=1C1OC2=CC(O)=CC(O)=C2CC1OC(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-UHFFFAOYSA-N 0.000 description 2
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 2
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 description 2
- 229940074393 chlorogenic acid Drugs 0.000 description 2
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 description 2
- 235000001368 chlorogenic acid Nutrition 0.000 description 2
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229940030275 epigallocatechin gallate Drugs 0.000 description 2
- 229940074391 gallic acid Drugs 0.000 description 2
- 235000004515 gallic acid Nutrition 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000007794 irritation Effects 0.000 description 2
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- 238000004659 sterilization and disinfection Methods 0.000 description 2
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- 229910001428 transition metal ion Inorganic materials 0.000 description 2
- YLZOPXRUQYQQID-UHFFFAOYSA-N 3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]propan-1-one Chemical compound N1N=NC=2CN(CCC=21)CCC(=O)N1CCN(CC1)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F YLZOPXRUQYQQID-UHFFFAOYSA-N 0.000 description 1
- 241000590002 Helicobacter pylori Species 0.000 description 1
- 241000207923 Lamiaceae Species 0.000 description 1
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000010634 clove oil Substances 0.000 description 1
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- 210000000805 cytoplasm Anatomy 0.000 description 1
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- 229940037467 helicobacter pylori Drugs 0.000 description 1
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
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- 229910021645 metal ion Inorganic materials 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
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- 229960003085 meticillin Drugs 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Description
まず、抗菌剤として、実施例1−1では、クローブの花蕾を乾燥させて粉砕した粉末200mgと、酸化マグネシウム50mgとを混合したものを用意し、実施例1−2では、ローズマリーの葉及び茎を乾燥させて粉砕した粉末200mgと、酸化マグネシウム50mgとを混合したものを用意した。次いで、大気中の細菌、微生物等を内容量約6リットルのデシケータ内に閉じ込め、シャーレにポリペプトン(タンパク質)1%溶液100cm3を栄養源として繁殖させた。続いて、シャーレに、用意した実施例1−1又は実施例1−2の抗菌剤をそれぞれ個別に添加した。そののち、デシケータ内において、細菌、微生物等がポリペクトンを酵素等の働きにより分解する際に発生する二酸化炭素の量をガステックを用いて測定した。二酸化炭素の増加は細菌、微生物の増加を意味している。
抗菌剤を添加しなかったことを除き、他は実施例1−1,1−2と同様にしてデシケータ内の二酸化炭素の量を測定した。
抗菌剤として、タイムの葉、茎及び花を乾燥させて粉砕した粉末200mgと、酸化マグネシウム50mgとを混合したものを添加したことを除き、他は実施例1−1,1−2と同様にしてデシケータ内の二酸化炭素の量を測定した。
抗菌剤として、セージの葉及び茎を乾燥させて粉砕した粉末200mgと、酸化マグネシウム50mgとを混合したものを添加したことを除き、他は実施例1−1,1−2と同様にしてデシケータ内の二酸化炭素の量を測定した。
実施例1−1,1−2及び比較例1−2,1−3の結果を図1に示す。図1に示したように、実施例1−1,1−2によれば、70時間を過ぎても二酸化炭素は増加しなかった。これに対して、図1には示していないが、抗菌剤を添加しなかった比較例1−1では、20時間を経過するまでは二酸化炭素の増加量は徐々であったが、20時間を経過したあたりから急激な増加が見られた。また、タイムを添加した比較例1−2、及び、セージを添加した比較例1−3では、50時間あたりから二酸化炭素の急激な増加が見られた。すなわち、クローブ及びローズマリーのうちの少なくとも1種と、酸化マグネシウムとを混合して抗菌剤として用いるようにすれば、高い抗菌効果を得られることが分かった。
実施例1−1,1−2の抗菌剤について、イネいもち病に対する種子消毒効果を調べた。供試植物はイネの栽培品種「はえぬき」を使用した。用いたイネの種子は、前年度いもち病菌を噴霧接種して作成したいもち病菌汚染種子である。実施例1−1,1−2の抗菌剤50mgと、脱塩水100mlとをそれぞれ混合し、個別にシャーレに入れて、そこにいもち病菌汚染種子を加え、25℃暗黒条件下で48時間浸種処理を行った。なお、抗菌剤は、実施例2−1では、クローブの粉末400mgと、酸化マグネシウム50mgとを混合したものを用い、実施例2−2では、ローズマリーの粉末400mgと、酸化マグネシウム50mgとを混合したものを用いた。
抗菌剤を添加しなかったことを除き、すなわち、脱塩水にいもち病菌汚染種子を浸種処理したことを除き、他は実施例2−1,2−2と同様にして種子発芽率と苗いもち発病率を調べた。
Claims (2)
- 枝、葉、花蕾を使用したクローブ粉末やオイル、それらからの抽出物の何れかと、酸化マグネシウムとを含むようにするか、または、枝、葉、花蕾を使用したクローブ粉末やオイル、それらからの抽出物の何れかに、葉、茎を使用したローズマリー粉末やオイル、それらからの抽出物の何れかを組み合せたものと、酸化マグネシウムとを含むようにするかしたことを特徴とする抗菌剤。
- 農業に用いることを特徴とする請求項1記載の抗菌剤。
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JP2013134151A JP5952783B2 (ja) | 2013-06-26 | 2013-06-26 | 抗菌剤 |
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JP5952783B2 true JP5952783B2 (ja) | 2016-07-13 |
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Family Cites Families (3)
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TR199801320T2 (xx) * | 1996-01-12 | 1998-10-21 | The Procter & Gamble Company | Dezenfekte edici bile�imler ve y�zeyleri dezenfekte etmek i�in i�lemler. |
FR2867948B1 (fr) * | 2004-03-29 | 2008-02-22 | Hightech Bio Activities Holdin | Composition decontaminante presentant a la fois des proprietes bactericides, fongicides et virucides, procedes d'obtention et utilisations d'une telle composition |
JP5796006B2 (ja) * | 2011-12-14 | 2015-10-21 | 有限会社 ワーコム農業研究所 | 抗菌剤 |
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