JP5890783B2 - 光学フローセル検出器 - Google Patents
光学フローセル検出器 Download PDFInfo
- Publication number
- JP5890783B2 JP5890783B2 JP2012551126A JP2012551126A JP5890783B2 JP 5890783 B2 JP5890783 B2 JP 5890783B2 JP 2012551126 A JP2012551126 A JP 2012551126A JP 2012551126 A JP2012551126 A JP 2012551126A JP 5890783 B2 JP5890783 B2 JP 5890783B2
- Authority
- JP
- Japan
- Prior art keywords
- flow cell
- optical waveguide
- light
- detector
- optical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000003287 optical effect Effects 0.000 title claims description 124
- 239000012530 fluid Substances 0.000 claims description 26
- 238000012544 monitoring process Methods 0.000 claims description 18
- 238000010521 absorption reaction Methods 0.000 claims description 12
- 239000013307 optical fiber Substances 0.000 claims description 10
- 230000031700 light absorption Effects 0.000 claims description 6
- 238000004891 communication Methods 0.000 claims description 2
- 238000001514 detection method Methods 0.000 description 9
- 239000000835 fiber Substances 0.000 description 9
- 238000000034 method Methods 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 230000035945 sensitivity Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000004448 titration Methods 0.000 description 2
- 238000010977 unit operation Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011026 diafiltration Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000011143 downstream manufacturing Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000013020 final formulation Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/05—Flow-through cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N2021/0346—Capillary cells; Microcells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/08—Optical fibres; light guides
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Description
・ピーク形状の特徴付けに基づくクロマトグラムのレビュープロセス及びプロセスの問題解決は、ピーク形状の正確な表示、したがってピーク先端までの直線的応答が必要となる。
・場合によって中間の試料の調整を伴う、2つ以上の単位操作の直接の結合への現在のトレンドがある。監督機関は、2つの単位操作の間の試料の正確なオンラインクロマトグラムの形での真の「プロセスフィンガープリント」を要求する場合がある。このようなプロセス整合性のフィンガープリントは、最も高いピーク濃度まで線形な検出器を必要とする。
・連続クロマトグラフィーでは、いくつかの同一のカラムが、カラムを直列及び/又は並列に動作させることを可能にする構成に接続され、個々のカラムは、従来技術によれば溶出のために時間をベースとするバルブ切り換えを用いて接続又は切断される。最近では同じ出願人によってSE0950494−5において溶出液信号をベースとする切り換え手法が提案されており、それによればカラムからの溶出液の組成を表す溶出液信号、たとえばUV吸光度信号が検出され、検出した信号に基づいて弁の切り換えが制御される。本発明による小ギャップ検出器は、最新の高滴定濃度供給装置に対する破過の検出を可能にする。
・最終定式化段階での限外濾過/透析濾過(ultra−filtration/Dia−filtration(UF/DF))時の高いタンパク質濃度の測定。
図7は、本発明の一実施形態による光学フローセル検出器の直線性を示す、実験に基づくグラフである。実験では、0.12mmの経路長を有するプロトタイプの光学フローセル検出器が、続いてポリクローナルhIgG(165mg/mlで開始)の希釈系列を用いて試験され、84mg/ml以上までの直線性を示す。
103 フローセル
105 光ファイバ
107 モノクロメータ
109 光スプリッタユニット
111 基準ファイバ
113 フローセルファイバ
115 光検出ユニット
117 基準入力
119 フローセル入力
121 光ファイバ
200 光学フローセル検出器
205 検出器本体
210 試料入口
220 試料出口
230 フローセル流路
240 入力光導波路
245 光学断面積
250 光出口面
260 光入口面
270 出力光導波路
280 フェルール
290 フェルール
295 シール
300 光学フローセル検出器
305 検出器本体
310 第1の内腔
315 試料入口
320 第2の内腔
325 試料出口
330 流体流路
335 第3の内腔
400 フローセル光吸収監視システム
410 光源
420 プリズム
430 基準光センサ
440 試料光センサ
460 ハウジング
Claims (6)
- 断面積Aのフローセル流路を通して流体連絡する試料入口及び出口と、出力光導波路の光入口面に隣接しそれと光学的に整列して配置された光出口面を有する入力光導波路とを備える光学フローセル検出器であって、前記入力光導波路及び前記出力光導波路が前記フローセル流路内に突き出しており、前記光出口面と前記光入口面の間の距離が0.02mm以上1.0mm未満であり、前記入力光導波路及び前記出力光導波路の突出部分の流れ方向と直交する方向の断面積がA/2未満であり、前記入力光導波路及び前記出力光導波路が、少なくとも停滞区域を超えて前記フローセル流路の内壁から突き出していて、前記入力光導波路の前記光出口面が前記出力光導波路の前記光入口面よりも小さな断面積を有しており、流れ断面積が前記フローセル全体にわたって流れ方向において事実上一様であり、前記入力光導波路及び前記出力光導波路が光ファイバから構成される、光学フローセル検出器。
- 前記光出口面と前記光入口面の間の距離が調整可能である、請求項1記載の光学フローセル検出器。
- 前記入力及び出力光導波路の少なくとも一方が、前記流体流路内に延在する支持フェルール内に配置される、請求項1又は請求項2記載の光学フローセル検出器。
- 第2の光出口面を有する第2の入力光導波路及び第2の光入口面を有する第2の出力光導波路をさらに備えていて、第2の光出口面と第2の光入口面との間の距離が、前記入力光導波路の光出口面と前記出力光導波路の光入口面との間の距離を超える、請求項1乃至請求項3のいずれか1項記載の光学フローセル検出器。
- 検出器本体であって、前記試料入口を形成する前記検出器本体の外面から延在する第1の内腔と、第1の内腔と同心でありそれより太く、第1の内腔から前記試料出口を形成する前記検出器本体の外面まで延在する第2の内腔と、前記検出器本体を貫通して延在し、前記第2の内腔と交差する第3の内腔とを有する検出器本体を備えており、前記入力光導波路及び前記出力光導波路は、それらが前記第2の内腔内に突き出るように、前記第3の内腔の対向する区間内に配置される、請求項1乃至請求項4のいずれか1項記載の光学フローセル検出器。
- 光源と、光検出器と、請求項1乃至請求項5のいずれか1項記載のフローセルとを備えるフローセル光吸収監視システムであって、UVから可視光までの波長領域における吸収を示すように構成されている、フローセル光吸収監視システム。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE1050090-8 | 2010-01-28 | ||
SE1050090 | 2010-01-28 | ||
PCT/SE2011/050078 WO2011093775A1 (en) | 2010-01-28 | 2011-01-26 | Optical flow cell detector |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2013518278A JP2013518278A (ja) | 2013-05-20 |
JP2013518278A5 JP2013518278A5 (ja) | 2014-03-13 |
JP5890783B2 true JP5890783B2 (ja) | 2016-03-22 |
Family
ID=44319579
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012551126A Active JP5890783B2 (ja) | 2010-01-28 | 2011-01-26 | 光学フローセル検出器 |
Country Status (5)
Country | Link |
---|---|
US (1) | US8649005B2 (ja) |
EP (1) | EP2529198B1 (ja) |
JP (1) | JP5890783B2 (ja) |
CN (1) | CN102713564B (ja) |
WO (1) | WO2011093775A1 (ja) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2512527B (en) * | 2011-12-02 | 2017-03-01 | Biochrom Ltd | Device for receiving small volume liquid samples |
GB201120769D0 (en) * | 2011-12-02 | 2012-01-11 | Biochrom Ltd | Improvements in and relating to devices for recieving liquid samples |
US9146189B2 (en) | 2014-02-28 | 2015-09-29 | Asl Analytical, Inc. | Optical cell with disposable fluid cartridge |
US9702813B2 (en) * | 2014-07-23 | 2017-07-11 | Infineon Technologies Ag | Sensing systems and methods using a coupling structure |
WO2016079797A1 (ja) * | 2014-11-18 | 2016-05-26 | 日本メクトロン株式会社 | インライン式濃度測定プローブ及び濃度測定システム |
US10185088B2 (en) * | 2014-12-18 | 2019-01-22 | Ge Healthcare Bio-Sciences Ab | Optical fiber arrangement for a system for measuring the light absorption or determining the concentration of a substance |
GB201506095D0 (en) * | 2015-04-10 | 2015-05-27 | Ge Healthcare Bio Sciences Ab | Optical flow cell for an optical measuring device |
GB201506132D0 (en) | 2015-04-10 | 2015-05-27 | Ge Healthcare Bio Sciences Ab | Device for holding a light guide, and method for manufacturing such a device |
KR101684407B1 (ko) * | 2015-05-22 | 2016-12-08 | 전남대학교산학협력단 | 광학 센서를 이용한 수질 오염 측정 시스템 및 수질 오염 측정 장치 |
EP3325944B1 (en) * | 2015-07-24 | 2019-12-11 | Hewlett-Packard Development Company, L.P. | Light guide for fluid testing cells |
CN105424603B (zh) * | 2015-12-14 | 2019-05-21 | 重庆川仪分析仪器有限公司 | 基于分光光度法的检测池总成 |
GB201603380D0 (en) | 2016-02-26 | 2016-04-13 | Ge Healthcare Bio Sciences Ab | Apparatus and method for measuring the light absorbance of a substance in a solution |
US10197494B2 (en) | 2016-06-23 | 2019-02-05 | Biocomp Instruments Inc. | Flow cell and system for simultaneous measurement of absorbance and emission in a sample |
GB201612010D0 (en) * | 2016-07-11 | 2016-08-24 | Ge Healthcare | A method and a measuring device for measuring the absorbance of a substance in a solution |
GB201620266D0 (en) * | 2016-11-30 | 2017-01-11 | Ge Healthcare Bio Sciences Ab | Optical flow cell |
EP3586123B1 (en) | 2017-02-21 | 2023-03-08 | Cytiva Sweden AB | Methods and systems for adapting pathlength and/or wavelength of a uv-absorbance cell in a chromatography system |
US10711237B2 (en) | 2017-08-22 | 2020-07-14 | Idex Health & Science Llc | Apparatus and methods for bioprocesses and other processes |
US10960393B2 (en) | 2017-08-22 | 2021-03-30 | Idex Health And Science Llc | Apparatus and methods for bioprocesses and other processes |
JP7113604B2 (ja) * | 2017-09-22 | 2022-08-05 | 株式会社Screenホールディングス | 吸光度測定装置および吸光度測定方法 |
GB201808748D0 (en) * | 2018-05-29 | 2018-07-11 | Ge Healthcare Bio Sciences Ab | Optical flow cell |
JP7360100B2 (ja) | 2018-09-25 | 2023-10-12 | 株式会社フジキン | 濃度測定装置 |
US20220276157A1 (en) * | 2019-08-06 | 2022-09-01 | Amgen Inc | Systems and methods for determining protein concentrations of unknown protein samples based on automated multi-wavelength calibration |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6134461U (ja) * | 1984-07-31 | 1986-03-03 | 修一 村田 | 体外循環回路内レ−ザ−気泡検出装置用セル |
JPS61182841U (ja) * | 1985-05-07 | 1986-11-14 | ||
ATE60133T1 (de) * | 1987-07-22 | 1991-02-15 | Ciba Geigy Ag | Prozesskuevette. |
EP0326511B1 (de) * | 1988-01-14 | 1992-02-26 | Ciba-Geigy Ag | Mikrodurchflusszelle |
US5140169A (en) * | 1991-04-25 | 1992-08-18 | Conoco Inc. | Long path flow cell resistant to corrosive environments for fiber optic spectroscopy |
JPH0593688A (ja) * | 1991-09-30 | 1993-04-16 | Shimadzu Corp | 分光分析装置 |
EP0597152A1 (en) * | 1992-11-13 | 1994-05-18 | Jean-Pierre Chervet | Optimizing capillary flow cells |
US5442437A (en) * | 1993-09-13 | 1995-08-15 | Atlantic Richfield Company | Sample cell and probe for spectrophotometer |
IL116972A0 (en) * | 1996-01-31 | 1996-05-14 | Israel Atomic Energy Comm | Optical flow cell for use in spectral analysis |
DE29607239U1 (de) * | 1996-04-23 | 1996-06-05 | J & M Analytische Mess- und Regeltechnik GmbH, 73431 Aalen | Kapillarhalter |
US6188813B1 (en) * | 1999-02-10 | 2001-02-13 | Waters Investments Limited | Flow cell, analyte measurement apparatus and methods related thereto |
MXPA02010596A (es) | 2000-04-26 | 2003-03-10 | Glaxo Group Ltd | Metodo y aparato para el analisis espectroscopico in -situ. |
US6542231B1 (en) * | 2000-08-22 | 2003-04-01 | Thermo Finnegan Llc | Fiber-coupled liquid sample analyzer with liquid flow cell |
US6600558B2 (en) * | 2000-08-22 | 2003-07-29 | Nippon Telegraph And Telephone Corporation | Micro-fluidic cell for optical detection of gases and method for producing same |
JP2002181694A (ja) * | 2000-12-13 | 2002-06-26 | Sekisui Chem Co Ltd | 分光光度計 |
JP2007113979A (ja) * | 2005-10-19 | 2007-05-10 | Ebara Corp | マルチ分光分析装置 |
JP4712745B2 (ja) * | 2007-03-06 | 2011-06-29 | 倉敷紡績株式会社 | 透過光測定用フローセル |
GB2460981B (en) * | 2007-04-13 | 2011-11-09 | Technologies Inc C | Interactive variable pathlength device |
-
2011
- 2011-01-26 JP JP2012551126A patent/JP5890783B2/ja active Active
- 2011-01-26 CN CN201180007580.1A patent/CN102713564B/zh active Active
- 2011-01-26 US US13/522,880 patent/US8649005B2/en active Active
- 2011-01-26 EP EP11737364.7A patent/EP2529198B1/en active Active
- 2011-01-26 WO PCT/SE2011/050078 patent/WO2011093775A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
US8649005B2 (en) | 2014-02-11 |
EP2529198B1 (en) | 2017-12-27 |
EP2529198A4 (en) | 2014-02-19 |
EP2529198A1 (en) | 2012-12-05 |
CN102713564B (zh) | 2016-07-20 |
CN102713564A (zh) | 2012-10-03 |
JP2013518278A (ja) | 2013-05-20 |
WO2011093775A1 (en) | 2011-08-04 |
US20120327397A1 (en) | 2012-12-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5890783B2 (ja) | 光学フローセル検出器 | |
US6108083A (en) | Spectroscopic systems for the analysis of small and very small quantities of substance | |
JP3657900B2 (ja) | 液体フローセルを伴ったファイバー結合液体サンプル分析器 | |
US5917606A (en) | Photometric flow apparatus for small sample volumes and method of making same | |
US20170292909A1 (en) | Flow cell optical detection system | |
US6385380B1 (en) | Hollow optical waveguide for trace analysis in aqueous solutions | |
US6963062B2 (en) | Method for multiplexed optical detection including a multimode optical fiber in which propagation modes are coupled | |
CN110325830B (zh) | 用于液相色谱的集成照射检测流动池 | |
US20140373606A1 (en) | Micromachined flow cell with freestanding fluidic tube | |
EP1564541A2 (en) | Dual pathlength system for light absorbance detection | |
US9146192B2 (en) | Integrated light scattering and ultraviolet absorption measurement system | |
JP2003518255A (ja) | 小型化された分光計 | |
US4475813A (en) | Divergent light optical systems for liquid chromatography | |
US20120069340A1 (en) | Flow cell exploiting radiation within cell wall | |
JP4712745B2 (ja) | 透過光測定用フローセル | |
US6930778B2 (en) | Microchemical system | |
JP2009250825A (ja) | 吸光度測定用プローブ及び吸光光度計 | |
Šesták et al. | Nanolitre-scale cell based on L-shaped silica capillary and optical fibre for absorption photometric detection in capillary liquid chromatography | |
US7420665B2 (en) | Optical detection device with reduced light throughput oscillations | |
CN104316629A (zh) | 一种液相多通道检测器装置 | |
KR20140103304A (ko) | 소 체적 액체 샘플을 수용하기 위한 장치 | |
JP2014032098A (ja) | 液体クロマトグラフ分析装置 | |
JPH02276927A (ja) | 一体型分光器とオンライン用分光計測装置 | |
JP7101991B2 (ja) | 少なくとも1つの溶液中の物質の吸光度を測定する方法及び測定装置 | |
JP7147952B2 (ja) | クロマトグラフ用検出器 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20140122 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20140122 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20140724 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20140729 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20141027 |
|
A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20141104 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20141211 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20150526 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20150825 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20160126 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20160219 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5890783 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
S533 | Written request for registration of change of name |
Free format text: JAPANESE INTERMEDIATE CODE: R313533 |
|
R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |