JP5884112B2 - Muscle enhancer - Google Patents
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- JP5884112B2 JP5884112B2 JP2014010506A JP2014010506A JP5884112B2 JP 5884112 B2 JP5884112 B2 JP 5884112B2 JP 2014010506 A JP2014010506 A JP 2014010506A JP 2014010506 A JP2014010506 A JP 2014010506A JP 5884112 B2 JP5884112 B2 JP 5884112B2
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Description
本発明は、安全に筋肉量を増大させることができる筋肉増強剤に関するものである。 The present invention relates to a muscle enhancer that can safely increase muscle mass.
加齢や運動不足あるいは運動の不能などの理由で歩行困難になる例は多い。また、筋肉量の減少によって発生する皺なども美容上の問題事項で、その解決策は知られていない。 There are many cases where walking is difficult due to aging, lack of exercise, or inability to exercise. In addition, wrinkles that occur due to a decrease in muscle mass are cosmetic problems, and no solution is known.
戦後、生活環境や食事内容の変化により、日本は世界の長寿国に仲間入りができた。しかし高齢者の心身の活動の低下に由来する社会問題として、介護や医療のあり方が注目を浴びている。高齢に達するまで元気で、生活習慣病などにも罹らず最終臥床期間の短い生き方ができることは、皆の望むところである。 After the war, Japan was able to join the world's longevity countries due to changes in the living environment and meal content. However, care and medical care are attracting attention as a social problem that stems from a decline in mental and physical activities of the elderly. Everyone wants to be healthy until they reach the age of life, and to have a life with a short final bed period without suffering from lifestyle-related diseases.
多細胞生物であるヒトの老化の最大の特徴として、まず細胞数の減少と細胞機能の低下が挙げられる。脳や心臓などの循環器系疾患で歩行が困難になると忽ち筋肉の委縮が誘発され、さらにQOL(Quality of Life)の悪化がみられる。 The greatest characteristic of aging in humans, which are multicellular organisms, is first a decrease in the number of cells and a decrease in cell function. When walking becomes difficult due to circulatory diseases such as the brain and heart, atrophy of the fallen muscle is induced, and QOL (Quality of Life) is further deteriorated.
そこまでいかなくても、老化と共に腕や首筋、顔などの筋肉の委縮による皺やタルミなどは身近な問題である。しかしながら、筋肉の委縮を抑制したり筋肉の増加を具体的に裏付ける実用的な合成医薬品はみられない。 Even if it does not go so far, wrinkles and talmi due to atrophy of arms, neck, face and other muscles with aging are familiar problems. However, there is no practical synthetic drug that suppresses muscle atrophy or specifically supports the increase in muscle.
筋肉を増強するための化合物としては、従来、ステロイドホルモンがよく知られている。しかし、筋肉増強目的でステロイドホルモンを服用したスポーツ選手に循環器疾患など重篤な副作用が生じることも知られている。 Conventionally, steroid hormones are well known as compounds for strengthening muscles. However, it is also known that serious side effects such as cardiovascular diseases occur in athletes who have taken steroid hormones for the purpose of muscle strengthening.
一方、安全な筋肉増強剤も模索されている。例えば特許文献1には、リンゴ由来のポリフェノールを有効成分とするものであり、廃用性筋萎縮による筋線維タイプの遅筋型TypeIから速筋型TypeIIへの移行を阻止したり、廃用性筋萎縮を抑制すると同時に萎縮した筋肉の回復時における筋肉量の回復を促進する製剤が開示されている。 On the other hand, a safe muscle enhancer is also being sought. For example, Patent Document 1 includes an apple-derived polyphenol as an active ingredient, which prevents the transition from a slow-muscle type I to a fast-muscle type II due to disuse muscle atrophy, A preparation that suppresses muscle atrophy and at the same time promotes the recovery of muscle mass at the time of recovery of a contracted muscle is disclosed.
上述したように、ステロイドホルモンなどとは異なる安全な筋肉増強剤が模索されており、リンゴ由来のポリフェノールを筋肉量の回復のために用いる特許文献1の技術も知られている。 As described above, a safe muscle enhancer different from steroid hormones has been sought, and the technique of Patent Document 1 using an apple-derived polyphenol for recovery of muscle mass is also known.
しかし特許文献1の技術は、あくまで廃用性筋萎縮により萎縮した筋肉のタイプ移行を抑制したり筋肉量を増やすための技術であり、例えば、健常人における筋肉量を増やしたり、加齢による皺などに効果を示すかは不明である。実際、特許文献1に記載されている実験データは、ラットの後肢を懸垂することにより筋萎縮を生じさせたモデル動物によるもののみである。 However, the technique of Patent Document 1 is a technique for suppressing the type transition of a muscle that has been atrophied due to disuse muscle atrophy or increasing the amount of muscle, for example, increasing the amount of muscle in a healthy person, It is unclear whether it will be effective. Actually, the experimental data described in Patent Document 1 is only from a model animal in which muscle atrophy is caused by hanging the hind limb of a rat.
以上の状況下、本発明は、安全であり、萎縮などによりいったん減少した筋肉量を回復するのみならず、一般的なヒトやその他の動物の筋肉量を増大させることができる筋肉増強剤を提供することを目的とする。 Under the circumstances described above, the present invention provides a muscle strengthening agent that is safe and can not only recover muscle mass once reduced by atrophy, but also increase muscle mass in general humans and other animals. The purpose is to do.
本発明者は、上記課題を解決するために鋭意研究を重ねた。例えば、サンシュユの果実、ナツメの果実、ネズミモチの果実、女貞子や市販の乾燥果実など、漢方薬などの中からいわゆる強壮生薬や老化予防に有用として知られている天然物の粉末を通常の飼育用飼料に加えて動物実験を行ったが、芳しい結果は得られなかった。特に、リンゴ果実圧搾残滓のみでは健常な実験動物の筋肉量を増大させるには不十分であった。しかし、リンゴ果実圧搾残滓に加えてローズマリー抽出物を併用すれば、健常者や健常な実験動物の筋肉量を有意に増大できることを見出して、本発明を完成した。 This inventor repeated earnest research in order to solve the said subject. For example, we use natural powders known to be useful for preventing aging and so-called tonic medicines, such as sanshuyu fruit, jujube fruit, rat peach fruit, Sadako and commercial dried fruit. Animal experiments were conducted in addition to the feed, but no good results were obtained. In particular, apple fruit press residue alone was insufficient to increase the muscle mass of healthy laboratory animals. However, the present inventors have found that the muscle mass of healthy individuals and healthy laboratory animals can be significantly increased if rosemary extract is used in combination with apple fruit press residue, and the present invention has been completed.
本発明に係る筋肉増強剤は、リンゴ果実圧搾残滓およびローズマリー抽出物を含有することを特徴とする。 The muscle strengthening agent which concerns on this invention contains an apple fruit pressing residue and a rosemary extract, It is characterized by the above-mentioned.
本発明の筋肉増強剤において、ローズマリー抽出物に対するリンゴ果実圧搾残滓の質量比は、5倍以上、15倍以下が好ましい。リンゴ果実圧搾残滓とローズマリー抽出物とをかかる割合で含む筋肉増強剤の効果は、本発明者の実験的知見により確認されている。 In the muscle strengthening agent of the present invention, the mass ratio of apple fruit press residue to rosemary extract is preferably 5 times or more and 15 times or less. The effect of the muscle strengthening agent containing apple fruit press residue and rosemary extract in such a ratio has been confirmed by the experimental knowledge of the present inventors.
本発明に係る筋肉増強剤の有効成分の一つであるローズマリー抽出物としては、ローズマリーの葉に由来するもの、および、抽出溶媒としてC1-4アルコールを用いて抽出されたものが好ましい。かかるローズマリー抽出物を含む本発明の筋肉増強剤の効果は、本発明者の実験的知見により確認されている。 As the rosemary extract which is one of the active ingredients of the muscle enhancing agent according to the present invention, those derived from rosemary leaves and those extracted using C 1-4 alcohol as an extraction solvent are preferable. . The effect of the muscle-enhancing agent of the present invention containing such a rosemary extract has been confirmed by the inventor's experimental findings.
本発明に係る筋肉増強剤の投与量としては、1日当たり50mg/kg体重以上、200mg/kg体重以下が好ましい。健常なヒトに対する実験によれば、この範囲の投与量で筋肉量の明らかな増加が認められた。 The dosage of the muscle enhancing agent according to the present invention is preferably 50 mg / kg body weight or more and 200 mg / kg body weight or less per day. According to experiments on healthy humans, a clear increase in muscle mass was observed at doses in this range.
本発明に係る筋肉増強剤は、食用にもされているリンゴと、ハーブティーやスパイスなどとして同じく食用にもされているローズマリー抽出物を有効成分とするので、毎日など恒常的な服用にも適する極めて安全なものである。また、本発明に係る筋肉増強剤は、例えば疾病や加齢などによる寝たきりの患者など筋肉の萎縮がみられる患者のみならず、健常なヒトやその他の動物の筋肉量を増大させることができる。さらには、筋肉量の減少を原因とする皺やタルミなどの改善も可能である。よって本発明に係る筋肉増強剤は、非常に実用的なものとして、産業上極めて有用である。 The muscle-strengthening agent according to the present invention contains apples that are also edible and rosemary extract that is also edible as herbal teas and spices as an active ingredient. Suitable and extremely safe. The muscle enhancer according to the present invention can increase the muscle mass of not only patients with muscle atrophy such as bedridden patients due to illness or aging, but also healthy humans and other animals. Furthermore, it is possible to improve wrinkles and tarmi caused by a decrease in muscle mass. Therefore, the muscle strengthening agent according to the present invention is extremely useful industrially as a very practical one.
本発明に係る筋肉増強剤は、有効成分として、リンゴ果実圧搾残滓およびローズマリー抽出物を含有することを特徴とする。 The muscle strengthening agent according to the present invention is characterized by containing apple fruit press residue and rosemary extract as active ingredients.
本発明に係る筋肉増強剤の有効成分の一つであるリンゴ果実圧搾残滓の原料であるリンゴは、特に制限されないが、例えば、より一般的に食用に付されている西洋リンゴ(Malus pumila)の果実のみならず、和リンゴ(Malus asiatica)の果実も用いることができる。また、使用するリンゴ果実の形態も特に制限されず、例えばカワ付きのまま用いることができるし、或いはカワを除去したもの、さらにカワと芯を除去したものなども用いることができる。 Although the apple which is a raw material of the apple fruit press residue which is one of the active ingredients of the muscle strengthening agent which concerns on this invention is not restrict | limited, For example, the western apple (Malus pumilla) more generally attached | subjected to edible is used. Not only fruit but also Japanese apple (Malus asiatica) fruit can be used. Moreover, the form of the apple fruit to be used is not particularly limited. For example, the apple fruit can be used as it is with the river, or the river from which the river has been removed, or the river and the core have been removed.
本発明において「リンゴ果実圧搾残滓」とは、リンゴを圧搾して果汁を除去した残り滓をいい、例えば、リンゴジュースの製造で得られる残り滓を利用することができる。その主要成分は、不溶性の食物繊維である。「食物繊維」とは、食物に含まれているものであり、ヒトの消化酵素で分解されない不消化性または難消化性の成分をいい、主に、植物食物や菌類植物の細胞壁を構成する多糖類からなるものいう。なお、リンゴ果実圧搾残滓は、リンゴファイバーやアップルファイバーの製品名で、乾燥物などとして市販されていることがある。 In the present invention, the “apple fruit pressing residue” refers to a remaining residue obtained by squeezing an apple to remove fruit juice. For example, a remaining residue obtained by manufacturing apple juice can be used. Its main component is insoluble dietary fiber. “Dietary fiber” is an indigestible or indigestible component that is contained in food and is not degraded by human digestive enzymes. It mainly consists of plant food and the cell walls of fungal plants. It consists of sugars. In addition, apple fruit pressing residue is a product name of apple fiber or apple fiber, and is sometimes marketed as a dried product.
本発明におけるリンゴ果実圧搾残滓は、リンゴを圧搾して果汁を除去した残り滓であればよく、例えば、リンゴ由来の食物繊維の他に水分、タンパク質、脂質、ミネラル、糖類を含むものであってもよい。また、果汁を完全に除去することは難しいので、本発明で使用するリンゴ果実圧搾残滓に果汁の一部やその乾燥物が残留していてもよいものとする。本発明のリンゴ果実圧搾残滓は、筋肉量の増大作用が発揮される範囲で食物繊維成分を含んでいればよく、具体的には、20質量%以上の食物繊維を含んでいることが好ましい。当該割合としては、25質量%以上がより好ましく、30質量%以上がさらに好ましい。一方、当該割合の上限は特に制限されず、100質量%でもよいが、原料であるリンゴ果実から食物繊維のみを分離するのが技術的に難しく製造コストが過剰にかかるおそれもあり得、また、食物繊維以外の成分が筋肉増強作用の発揮に寄与している可能性もあるので、当該割合としては、90質量%以下が好ましく、80質量%以下がより好ましく、70質量%以下がさらに好ましく、60質量%以下がさらに好ましく、50質量%以下がさらに好ましい。 The apple fruit pressing residue in the present invention may be any remaining residue obtained by pressing the apple and removing the fruit juice, and includes, for example, moisture, protein, lipid, mineral, and sugar in addition to dietary fiber derived from apple. Also good. Moreover, since it is difficult to remove fruit juice completely, a part of fruit juice and its dried product may remain in the apple fruit pressing residue used by this invention. The apple fruit press residue of this invention should just contain the dietary fiber component in the range by which the increase effect of muscle mass is exhibited, Specifically, it is preferable that the dietary fiber contains 20 mass% or more. As the said ratio, 25 mass% or more is more preferable, and 30 mass% or more is further more preferable. On the other hand, the upper limit of the ratio is not particularly limited, and may be 100% by mass, but it may be technically difficult to separate only dietary fiber from the raw material apple fruit, and the production cost may be excessive, Since components other than dietary fiber may contribute to the exertion of muscle strengthening action, the proportion is preferably 90% by mass or less, more preferably 80% by mass or less, further preferably 70% by mass or less, 60 mass% or less is more preferable, and 50 mass% or less is further more preferable.
リンゴ果実圧搾残滓の製造方法は特に制限されないが、例えば、リンゴジュースの製造などのためにリンゴ果実を圧搾した残滓を用いることができる。さらに、当該残滓を乾燥し、水分量を4質量%以上、10質量%以下にしたものを用いてもよい。当該水分量は、例えば、常圧加熱乾燥法により測定することができる。 Although the manufacturing method in particular of apple fruit pressing residue is not restrict | limited, For example, the residue which pressed apple fruit for manufacture of apple juice etc. can be used. Furthermore, you may use the said residue dried and water content made 4 mass% or more and 10 mass% or less. The water content can be measured by, for example, a normal pressure heating drying method.
ローズマリー(Rosmarinus officinalis,シソ科)は小型の常緑灌木で、厚い芳香性のある葉を持つ高さ約2m程度のスパイス原料としてよく知られた植物である。地中海地方が原産とされ、アメリカ、イギリス、フランス、スペインなど世界中で栽培されている。ローズマリーに含まれる成分としては、油脂やフラボノイド類の他、フェノール性化合物、トリテルペノイド化合物など多くの成分の報告が知られている。また、その抽出物の胆汁管や小腸への作用や、冠動脈の血流増加作用なども報告されている。ヨーロッパの植物療法では、ローズマリーの葉は消化不良やリウマチ、循環器系疾患に外用もされている。浴用剤としても用いられている。食品では、アルコール飲料、焼き菓子、冷凍乳製品、肉や肉製品調味料、薬味、加工野菜、スナック菓子、グレービーソースなど、多方面で使用されている。また、ハーブティーとして健胃効果を期待して用いられている薬草でもある。中国では葉や枝の抽出物が頭痛にも用いられている。 Rosemary (Rosmarinus officinalis, Lamiaceae) is a small evergreen shrub that is well known as a spice raw material with a thick aromatic leaf and a height of about 2 m. Originated in the Mediterranean region, it is cultivated all over the world, including the United States, England, France and Spain. As components contained in rosemary, many components such as phenolic compounds and triterpenoid compounds are known in addition to fats and oils and flavonoids. In addition, the action of the extract on the bile duct and small intestine and the blood flow increasing action of the coronary arteries have been reported. In European phytotherapy, rosemary leaves are also used for indigestion, rheumatism, and cardiovascular diseases. It is also used as a bath agent. In food, it is used in many fields, including alcoholic beverages, baked confectionery, frozen dairy products, meat and meat product seasonings, condiments, processed vegetables, snacks, and gravy. It is also a medicinal herb that is used as a herbal tea in anticipation of a healthy stomach effect. In China, leaf and branch extracts are also used for headaches.
ローズマリー抽出物の原料部位としては、葉および/または枝が好ましく、葉がより好ましい。また、ローズマリーは香りの強い油脂成分を含むので、原料としてローズマリーの葉などを直接用いると、得られる抽出物の香りが強く服用し難いものになる場合があり得る。よって、ローズマリー油を製造した後の残滓など、油脂成分を水蒸気蒸留などで除去した後の残滓を原料として用いてもよい。 As a raw material part of the rosemary extract, leaves and / or branches are preferable, and leaves are more preferable. In addition, since rosemary contains a fragrant oil and fat component, directly using rosemary leaves or the like as a raw material may result in a strong fragrance of the resulting extract that is difficult to take. Therefore, you may use the residue after removing fats and oils components, such as the residue after manufacturing rosemary oil, by steam distillation.
抽出溶媒としては、水溶性有機溶媒および水と水溶性有機溶媒との混合溶媒が好ましい。水溶性有機溶媒は特に制限されず、例えば、リンゴ圧搾残滓との組合せで優れた筋肉増強作用を発揮できるローズマリー抽出物が得られるものを適宜選択すればよいが、例えば、メタノール、エタノール、1−プロパノール、2−プロパノール、n−ブタノール、イソブタノール、s−ブタノール、t−ブタノールのC1-4アルコール;ジエチルエーテルやテトラヒドロフランなどの水溶性エーテル系溶媒;ジメチルホルムアミドやジメチルアセトアミドなどの水溶性アミド系溶媒;ジメチルスルホキシドなどの水溶性スルホキシド溶媒を挙げることができる。これらの中でも、安全性などの面からC1-4アルコールが好ましく、C1-2アルコールがより好ましく、エタノールがさらに好ましい。 As the extraction solvent, a water-soluble organic solvent and a mixed solvent of water and a water-soluble organic solvent are preferable. The water-soluble organic solvent is not particularly limited, and may be appropriately selected from those that can provide a rosemary extract capable of exerting an excellent muscle-enhancing effect in combination with apple crush residue, for example, methanol, ethanol, 1 -C 1-4 alcohols of propanol, 2-propanol, n-butanol, isobutanol, s-butanol, t-butanol; water-soluble ether solvents such as diethyl ether and tetrahydrofuran; water-soluble amides such as dimethylformamide and dimethylacetamide Examples of the solvent include water-soluble sulfoxide solvents such as dimethyl sulfoxide. Among these, C 1-4 alcohol is preferable from the viewpoint of safety and the like, C 1-2 alcohol is more preferable, and ethanol is more preferable.
水と水溶性有機溶媒との混合溶媒を用いる場合、全体に対する水溶性有機溶媒の濃度は適宜調整すればよいが、例えば、50v/v%以上とすることができ、60v/v%以上が好ましく、70v/v%以上がより好ましく、80v/v%以上がさらに好ましく、90v/v%以上が特に好ましい。上限は特に制限されないが、例えば、99v/v%以下とすることができる。 When a mixed solvent of water and a water-soluble organic solvent is used, the concentration of the water-soluble organic solvent with respect to the whole may be adjusted as appropriate. For example, it can be 50 v / v% or more, preferably 60 v / v% or more. 70 v / v% or more is more preferable, 80 v / v% or more is further preferable, and 90 v / v% or more is particularly preferable. The upper limit is not particularly limited, but can be, for example, 99 v / v% or less.
抽出溶媒の使用量は特に制限されず、適宜調整すればよい。例えば、ローズマリーに対して、2質量倍以上、20質量倍以下程度とすることができる。当該割合としては、5質量倍以上が好ましく、7質量倍以上がより好ましく、また、15質量倍以下が好ましく、12質量倍以下がより好ましい。 The amount of the extraction solvent used is not particularly limited and may be adjusted as appropriate. For example, it can be about 2 mass times or more and 20 mass times or less with respect to rosemary. The ratio is preferably 5 times or more, more preferably 7 times or more, more preferably 15 times or less, and more preferably 12 times or less.
抽出温度も適宜調整することができ、例えば、10℃以上、80℃以下とすることができる。なお、溶媒の沸点以上で抽出する場合には、抽出容器を密閉するか、加圧することが好ましい。また、抽出溶媒を加熱還流してもよい。さらに、抽出温度は段階的に変更してもよい。 The extraction temperature can also be adjusted as appropriate, and can be, for example, 10 ° C. or higher and 80 ° C. or lower. In addition, when extracting above the boiling point of a solvent, it is preferable to seal or pressurize an extraction container. Further, the extraction solvent may be heated to reflux. Further, the extraction temperature may be changed in stages.
抽出時間も適宜調整することができ、例えば、合計で1時間以上、50時間以下程度とすることができる。 The extraction time can also be adjusted as appropriate. For example, the extraction time can be about 1 hour or more and 50 hours or less in total.
抽出後の混合液は、そのまま用いることができるが、通常の後処理を行ってもよい。例えば、抽出残渣と抽出溶媒を濾過や遠心分離などにより分離したり、また、分離した抽出液を、加熱乾燥、減圧乾燥、凍結乾燥などを適宜組み合わせて乾燥してもよい。なお、加熱により乾燥する場合には、有効成分が分解しないよう60℃以下、好ましくは50℃以下で、減圧しつつ濃縮することが好ましい。 The extracted liquid mixture can be used as it is, but normal post-treatment may be performed. For example, the extraction residue and the extraction solvent may be separated by filtration or centrifugation, or the separated extract may be dried by appropriately combining heating drying, reduced pressure drying, freeze drying, and the like. In addition, when drying by heating, it is preferable to concentrate at 60 ° C. or less, preferably 50 ° C. or less, while reducing the pressure, so that the active ingredient does not decompose.
得られたローズマリー抽出物は、特に油脂成分を除去しないまま抽出を行った場合には、主に油脂成分を除去するために部分精製してもよい。例えば、抽出液を濃縮したり、抽出液に貧溶媒を加えるなどして析出する不溶成分を濾別して洗浄すれば、主な油脂成分は常温で液体であるので除去することができる。 The obtained rosemary extract may be partially purified mainly to remove the fat and oil component, particularly when extraction is performed without removing the fat and oil component. For example, if the extract is concentrated or an insoluble component that precipitates by adding a poor solvent to the extract is filtered and washed, the main oil component can be removed because it is liquid at room temperature.
本発明におけるリンゴ果実圧搾残滓とローズマリー抽出物との割合は、筋肉量の増大作用が発揮される範囲で適宜調整すればよいが、例えば、ローズマリー抽出物に対するリンゴ果実圧搾残滓の質量比を5倍以上、15倍以下とすることができる。当該質量比としては、6倍以上が好ましく、7倍以上がより好ましく、8倍以上がさらに好ましく、また、14倍以下が好ましく、13倍以下がより好ましく、12倍以下がさらに好ましい。なお、上記質量比は、リンゴ果実圧搾残滓またはローズマリー抽出物の乾燥度合いにより変化するので、リンゴ果実圧搾残滓およびローズマリー抽出物の水分含量を5質量%以上、6質量%以下に調整した上で算出するものとする。 The ratio of apple fruit press residue and rosemary extract in the present invention may be appropriately adjusted within the range in which the effect of increasing muscle mass is exerted, for example, the mass ratio of apple fruit press residue to rosemary extract It can be 5 times or more and 15 times or less. The mass ratio is preferably 6 times or more, more preferably 7 times or more, further preferably 8 times or more, further preferably 14 times or less, more preferably 13 times or less, and further preferably 12 times or less. In addition, since the said mass ratio changes with the drying degree of an apple fruit press residue or a rosemary extract, after adjusting the water content of an apple fruit press residue and a rosemary extract to 5 mass% or more and 6 mass% or less It shall be calculated by
本発明に係る筋肉増強剤の剤形は特に問わない。例えば、リンゴ果実圧搾残滓とローズマリー抽出物の乾燥体を単に混合したものであってもよいし、リンゴ果実圧搾残滓とローズマリー抽出物が水に分散または溶解しているものであってもよい。また、ローズマリーの葉が入浴剤として用いられたり、ローズマリー由来の精油や抽出物が外用剤や、石鹸、化粧品などの香料として用いられることもあるが、後記の実施例のとおり本発明に係る筋肉増強剤は経口投与で高い効果を示し、また、摂取や服用のし易さからも、経口剤とすることが好ましい。 The dosage form of the muscle strengthening agent according to the present invention is not particularly limited. For example, the apple fruit press residue and the dried rosemary extract may be simply mixed, or the apple fruit press residue and rosemary extract may be dispersed or dissolved in water. . In addition, rosemary leaves may be used as bathing agents, and rosemary-derived essential oils and extracts may be used as perfumes for external preparations, soaps, cosmetics, etc. Such a muscle strengthening agent is highly effective when orally administered, and is preferably an oral agent from the viewpoint of ease of ingestion and administration.
経口製剤としては、特に制限されないが、例えば、錠剤、散剤、カプセル剤、糖衣錠、顆粒剤などを挙げることができる。本発明に係る筋肉増強剤には、剤形に合わせ、薬学上許容される添加剤を用いてもよい。かかる添加剤としては、例えば、賦形剤、基剤、防腐剤、助剤、安定化剤、湿潤剤、pH調整剤、酸化防止剤、着色剤、甘味料などを挙げることができる。但し、本発明に係る筋肉増強剤の有効成分であるリンゴ果実圧搾残滓とローズマリー抽出物の乾燥体は、賦形剤などを添加しなくても造粒可能である。また、懸濁液を飲料にしてもよいし、一般的な食餌に添加してもよい。 Although it does not restrict | limit especially as an oral formulation, For example, a tablet, a powder, a capsule, a sugar-coated tablet, a granule etc. can be mentioned. For the muscle strengthening agent according to the present invention, a pharmaceutically acceptable additive may be used in accordance with the dosage form. Examples of such additives include excipients, bases, preservatives, auxiliaries, stabilizers, wetting agents, pH adjusting agents, antioxidants, colorants, sweeteners and the like. However, the dried apple fruit press residue and rosemary extract, which are the active ingredients of the muscle enhancer according to the present invention, can be granulated without adding excipients or the like. In addition, the suspension may be a beverage or may be added to a general diet.
本発明に係る筋肉増強剤は、ヒトに加えて、ヒト以外の動物にも投与可能である。例えば、ヒトと同様に加齢により運動が難しくなった愛玩動物の筋肉量の増加のためにも用いることができる。但し、好適にはヒトに対して用いる。 The muscle strengthening agent according to the present invention can be administered to animals other than humans in addition to humans. For example, it can also be used to increase the muscle mass of pets that have become difficult to exercise due to aging as in humans. However, it is preferably used for humans.
本発明に係る筋肉増強剤の投与頻度や投与量は、改善対象が深刻な筋肉量の低下であるか或いは単なる皺やタルミであるかや、患者の年齢、性別、状態などに応じて適宜調整すればよい。後記の実験データによれば、ヒトの体重(kg)当たり、1日約130mgの乾燥体投与で有意な筋肉量増大効果が確認された。かかる結果より、例えばヒトに対する投与量は、乾燥状態のリンゴ果実圧搾残滓−ローズマリー抽出物混合物で、1日当たり50mg/kg体重以上、200mg/kg体重以下程度とすることが好ましい。また、一日当たりの投与回数としては、1回以上、5回以下が好ましく、1回以上、3回以下がより好ましい。 The frequency and amount of administration of the muscle-enhancing agent according to the present invention are appropriately adjusted according to whether the improvement target is severe muscle loss or mere sputum or talmi, the patient's age, sex, condition, etc. do it. According to the experimental data described later, a significant muscle mass increasing effect was confirmed by administration of about 130 mg / day of dry body per body weight (kg) of human. From this result, for example, the dosage for humans is preferably about 50 mg / kg body weight or more and 200 mg / kg body weight or less per day in a dried apple fruit press residue-rosemary extract mixture. The number of administrations per day is preferably 1 or more and 5 or less, more preferably 1 or more and 3 or less.
以下、実施例を挙げて本発明をより具体的に説明するが、本発明はもとより下記実施例によって制限を受けるものではなく、前・後記の趣旨に適合し得る範囲で適当に変更を加えて実施することも勿論可能であり、それらはいずれも本発明の技術的範囲に包含される。 EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited by the following examples, but may be appropriately modified within a range that can meet the purpose described above and below. Of course, it is possible to implement them, and they are all included in the technical scope of the present invention.
実施例1:本発明に係る筋肉増強剤の調製
和リンゴ(Malus asiatica)および西洋リンゴ(Malus pumila)の果実を圧搾してリンゴジュースを製造する際に生じる圧搾残滓(株式会社秋田ニューバイオファームより恵与)を常圧下、90℃で3日間温風乾燥し、水分量を5〜6質量%の範囲に調整することによりリンゴ果実圧搾残滓を得た。
Example 1: Preparation of a muscle strengthening agent according to the present invention Pressed residue produced from squeezing fruit of Japanese apple (Malus asiatica) and Western apple (Malus pumila) to produce apple juice (from Akita New Bio Farm Co., Ltd.) Eiyo) was dried with warm air at 90 ° C. for 3 days under normal pressure, and the moisture content was adjusted to a range of 5 to 6% by mass to obtain apple fruit pressed residue.
得られたリンゴ果実圧搾残滓の組成分析を、一般財団法人日本食品分析センターに依頼した。結果を表1に示す。 The composition analysis of the obtained apple fruit pressing residue was requested to the Japan Food Analysis Center. The results are shown in Table 1.
上記結果のとおり、得られたリンゴ果実圧搾残滓には食物繊維が約30質量%含まれていた。また、ペクチンが約10〜20質量%含まれていた。 As the above result, the obtained apple fruit press residue contained about 30% by mass of dietary fiber. Moreover, about 10-20 mass% of pectin was contained.
また、健康食品素材として市販されている粉末状ローズマリー抽出物(サビンサジャパン・コーポレーション製,製品名「Rosemary extract」)を購入して用いた。当該抽出物は、溶媒としてエタノールを用いてローズマリー葉から加温抽出されたものであり、油脂成分が除去されたものである。 In addition, a commercially available powdered rosemary extract (manufactured by Sabinsa Japan Corporation, product name “Rosemary extract”) as a health food material was purchased and used. The said extract is what was heated and extracted from the rosemary leaf using ethanol as a solvent, and the fat and oil component was removed.
マウスを使った予備実験の結果、食餌内容や運動量などを特に指定や制限することなく飼育した場合には、リンゴ果実圧搾残滓:ローズマリー抽出物の混合割合が質量比で10:1の混合物が最も筋肉増量効果が優れていることが認められた。よって、上記リンゴ果実圧搾残滓とローズマリー抽出物を10:1(質量比)で混合した混合物を、以下の実験で用いた。 As a result of preliminary experiments using mice, when the food content and the amount of exercise were reared without any particular designation or restriction, a mixture of apple fruit compressed residue: rosemary extract mixing ratio of 10: 1 by mass ratio was obtained. It was recognized that the effect of increasing muscle mass was the most excellent. Therefore, the mixture which mixed the said apple fruit pressing residue and rosemary extract by 10: 1 (mass ratio) was used in the following experiment.
実施例2:実験動物を用いた筋肉増強作用試験
通常飼料(オリエンタル酵母社製,製品名「MF粉末」)に、上記リンゴ果実圧搾残滓−ローズマリー抽出物混合物を2質量%または10質量%添加し、混合した。また、比較のために、上記通常飼料に上記リンゴ果実圧搾残滓を10質量%添加混合した比較飼料も調製した。
Example 2: Muscle enhancement effect test using experimental animals 2% by mass or 10% by mass of the above-mentioned apple fruit pressed residue-rosemary extract mixture is added to normal feed (product name “MF powder” manufactured by Oriental Yeast Co., Ltd.) And mixed. For comparison, a comparative feed was prepared by adding 10% by mass of the apple fruit press residue to the normal feed.
体重20〜22gの雄性dd−Y系マウス(株式会社紀和実験動物研究所より入手)20匹を任意に5匹ずつ、通常飼料群(対照群)、検体2%添加群、検体10%添加群、比較飼料群に分け、それぞれ通常飼料、検体を2質量%添加した同通常飼料、検体を10質量%添加した同通常飼料、またはリンゴ果実圧搾残滓のみを10質量%添加した同通常飼料と水とを自由摂取させた。各マウスは、明暗12時間サイクル、温度22±1℃、湿度55〜65%の実験室で1匹ずつ個別に飼育した。実験開始から5週間目にマウスを屠殺し、各臓器の質量、睾丸周囲の脂肪量および後肢筋肉量を測定した。飼料と水の総摂取量および体重の変化を図1に、各臓器の質量を図2と図3に、睾丸周囲の脂肪量を図4に、後肢筋肉量を図5に示す。 20 male dd-Y mice weighing 20 to 22 g (obtained from Kiwa Laboratory Animal Research Co., Ltd.), 5 each arbitrarily, normal feed group (control group), sample 2% addition group, sample 10% addition group Divided into comparative feed groups, the normal feed, the same normal feed added with 2% by weight of the specimen, the same normal feed added with 10% by weight of the specimen, or the same normal feed and water added with only 10% by weight of apple fruit press residue And were allowed to eat freely. Each mouse was individually raised in a laboratory with a 12 hour light / dark cycle, a temperature of 22 ± 1 ° C., and a humidity of 55 to 65%. Five weeks after the start of the experiment, the mice were sacrificed, and the mass of each organ, the fat mass around the testicles, and the hindlimb muscle mass were measured. Changes in the total intake and body weight of feed and water are shown in FIG. 1, the mass of each organ is shown in FIGS. 2 and 3, the fat mass around the testicles is shown in FIG. 4, and the hindlimb muscle mass is shown in FIG.
図1のとおり、飼料の総摂取量、水の総摂取量、体重の変化において特に異常な個体はみられず、対照群、検体投与群、比較飼料投与群の間で有意な差は認められなかった。 As shown in FIG. 1, no abnormal individuals were observed in the total intake of feed, total intake of water, and body weight, and significant differences were observed among the control group, the sample administration group, and the comparative feed administration group. There wasn't.
各臓器の質量についても、図2と図3のとおり、特に異常な個体はみられず、対照群、検体投与群、比較飼料投与群の間で有意な差は認められなかった。なお、マウスの寿命を3年、ヒトの寿命を90年と仮定し、5週間にわたる連続投与をヒトに適用させた場合、本発明に係る筋肉増強剤は、少なくとも150週間(約2.8年間)摂取しても異常を生じない安全なものであると推定される。 As for the mass of each organ, as shown in FIGS. 2 and 3, no particularly abnormal individuals were observed, and no significant difference was observed among the control group, the sample administration group, and the comparative feed administration group. In addition, assuming that the lifespan of mice is 3 years and the lifespan of humans is 90 years and the continuous administration over 5 weeks is applied to humans, the muscle potentiator according to the present invention is at least 150 weeks (about 2.8 years). ) It is presumed that it is safe without causing any abnormality even if ingested.
睾丸周囲の脂肪量については、図4のとおり、本発明に係る筋肉増強剤を投与された群では、対照群に対して明らかに減少していた。特に本発明に係る筋肉増強剤を10質量%添加した飼料を摂取させた群では、tテストによる有意差検定でp<0.05で対照群に対する有意差が認められ、その平均脂肪量は対照群に比べて42%も減少した。 As shown in FIG. 4, the amount of fat around the testicle was clearly reduced in the group administered with the muscle-enhancing agent according to the present invention compared to the control group. In particular, in the group fed with 10% by weight of the muscle-enhancing agent according to the present invention, a significant difference from the control group was found at p <0.05 by the significant difference test by the t test, and the average fat amount was the control. There was a 42% decrease compared to the group.
後肢筋肉量については、図5のとおり、本発明に係る筋肉増強剤を10質量%添加した飼料を摂取させた群では、tテストによる有意差検定でp<0.05で対照群に対する有意な筋肉増強作用が認められた。一方、リンゴ果実圧搾残滓のみを添加した飼料を摂取させた群では、その添加割合が10質量%であっても、筋肉増強作用は認められなかった。 As for the hind limb muscle mass, as shown in FIG. 5, in the group fed with 10% by mass of the muscle-enhancing agent according to the present invention, p <0.05 in the significant difference test by the t test and significant for the control group. Muscle strengthening effect was observed. On the other hand, in the group which ingested the feed which added only the apple fruit pressing residue, the muscle reinforcement | strengthening effect was not recognized even if the addition ratio was 10 mass%.
以上の結果のとおり、本発明に係る筋肉増強剤を投与した場合には、通常飼料のみを摂取した対照群とリンゴ果実圧搾残滓のみが配合された飼料を摂取した比較飼料投与群に比べ、体重には相違が認められない一方で、脂肪量が低減され且つ筋肉量が増加した。かかる結果から、本発明に係る筋肉増強剤によれば、蓄積されている脂肪の代謝経路を変化させて筋肉量を増加させる遺伝子の発現が促進されることが考えられる。 As described above, when the muscle-enhancing agent according to the present invention was administered, the body weight compared to the control group ingested only the normal feed and the comparative feed administration group ingested the feed containing only the apple fruit compressed residue While no difference was observed, fat mass was reduced and muscle mass increased. From these results, it is considered that the muscle enhancer according to the present invention promotes the expression of genes that increase the muscle mass by changing the metabolic pathway of accumulated fat.
実施例3:ヒトに対する筋肉増強作用試験
次に、ヒトでの実験で筋肉増強作用の有無を検討した。56〜78歳の被験者5名(男性2名,女性3名)に、上記実施例1と同様のリンゴ果実圧搾残滓:ローズマリー抽出物=10:1(質量比)の混合物2gを、1日3回食前に服用させた。また、対照として、別の48〜72歳の被検者5名(男性1名,女性4名)に、代表的な水溶性食物繊維であるイヌリンを同様に服用させた。試験期間中、被験者には特別な運動などをさせることはなく、また、上記の服用以外は食事内容を変化させることなく、試験開始以前と同様の生活をしてもらった。試験開始時と2ヶ月後、デュアル周波数体組成計(タニタ社製,DC−320)を用い、脚部筋肉量点数を測定した。対照群と本発明に係る筋肉増強剤の服用群における試験開始時と2ヶ月後の脚部筋肉量点数の変化を図6に示す。
Example 3 Muscle Strengthening Action Test on Human Next, the presence or absence of muscle strengthening action was examined in human experiments. 5 subjects (2 men and 3 women) aged 56 to 78 years were given 2 g of a mixture of apple fruit pressing residue: rosemary extract = 10: 1 (mass ratio) as in Example 1 above. 3 times before meals. Further, as a control, another 48-72 year-old subject (1 male, 4 female) was similarly given inulin, which is a typical water-soluble dietary fiber. During the test period, the subject did not have any special exercise, and had the same life as before the start of the test without changing the food contents except for the above-mentioned use. At the start of the test and 2 months later, the leg muscle mass score was measured using a dual frequency body composition meter (Tanita, DC-320). FIG. 6 shows changes in leg muscle mass scores at the start of the test and after 2 months in the control group and the group taking the muscle strengthening agent according to the present invention.
図6の結果のとおり、特別な食事や運動などによらなくても、本発明に係る筋肉増強剤により、ヒトにおいても筋肉量を増加させる傾向のあることが実験的に証明された。 As shown in the results of FIG. 6, it was experimentally proved that there is a tendency to increase muscle mass even in humans with the muscle-enhancing agent according to the present invention without depending on a special meal or exercise.
Claims (6)
ローズマリー抽出物とリンゴ果実圧搾残滓とを混合する工程を含むことを特徴とする筋肉増強剤の製造方法。The manufacturing method of the muscle strengthening agent characterized by including the process of mixing a rosemary extract and apple fruit pressing residue.
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