JP5836127B2 - Fat-soluble vitamin-containing composition - Google Patents
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Description
本発明は、安定性が向上した脂溶性ビタミン含有組成物に関する。 The present invention relates to a fat-soluble vitamin-containing composition with improved stability.
従来、脂溶性ビタミンは、老化防止、美容効果、健康保持などに有効な成分として古くから注目され、健康食品、機能性食品、機能性飲料、サプリメント、医薬品、化粧品などに用いられている。しかし脂溶性ビタミンは、酸素、光、熱、ｐＨ、金属イオンなどに対して非常に敏感であり不安定であるため、保管中に脂溶性ビタミンが容易に分解して脂溶性ビタミン含量が低下するという問題があり、安定性の高い脂溶性ビタミン含有組成物が求められていた。 Conventionally, fat-soluble vitamins have been attracting attention as an effective ingredient for anti-aging, beauty effects, health maintenance and the like, and have been used for health foods, functional foods, functional beverages, supplements, pharmaceuticals, cosmetics and the like. However, fat-soluble vitamins are very sensitive and unstable to oxygen, light, heat, pH, metal ions, etc., so that fat-soluble vitamins are easily degraded during storage, resulting in a decrease in fat-soluble vitamin content. Therefore, a highly stable fat-soluble vitamin-containing composition has been demanded.
脂溶性ビタミンの安定性向上に関する従来技術としては、油中水中油型エマルジョンを配合してなる脂溶性ビタミン類含有飲食品であって、油中水中油型エマルジョンが内部油相部と水相部と外部油相部とからなり、内部油相部が脂溶性ビタミン類を含有する飲食品（特許文献１参照）、カロテノイドを含有する、安定化された油溶性ビタミン含有組成物（特許文献２参照）、光に不安定な脂溶性薬物に黄色及び赤色の着色剤から選ばれる１種以上の物質を配合してなる光安定性の向上した組成物（特許文献３参照）、コハク酸トコフェロールまたはその塩、ビタミンＢ１類またはその塩および比容が約３ｍＬ／ｇ以上である塩基性無機化合物を配合してなるビタミン製剤（特許文献４参照）などが開示されているが、いずれも脂溶性ビタミンの安定性向上の効果は十分ではなく更に好ましい方法が求められていた。 A conventional technique for improving the stability of fat-soluble vitamins is a fat-soluble vitamin-containing food or drink comprising an oil-in-water emulsion, wherein the oil-in-water emulsion contains an internal oil phase part and an aqueous phase part. And the external oil phase part, and the internal oil phase part contains fat-soluble vitamins (see Patent Document 1), stabilized oil-soluble vitamin-containing composition containing carotenoids (see Patent Document 2) ), A composition having improved photostability obtained by blending one or more substances selected from yellow and red colorants into a photolabile fat-soluble drug (see Patent Document 3), tocopherol succinate or the like Vitamin preparations (see Patent Document 4) comprising a salt, vitamin B1 or a salt thereof and a basic inorganic compound having a specific volume of about 3 mL / g or more have been disclosed. The effect of improving the stability further preferred method not enough has been demanded.
本発明の目的は、安定性が向上した脂溶性ビタミン含有組成物を提供することである。 The object of the present invention is to provide a fat-soluble vitamin-containing composition with improved stability.
本発明者は、上記課題を解決するために鋭意研究を重ねた結果、脂溶性ビタミンに炭酸カリウムを添加することにより上記課題を解決することを見出した。本発明者は、これらの知見に基づきさらに研究を重ね、本発明を完成するに至った。
すなわち、本発明は、
１．脂溶性ビタミンと炭酸カリウムとを含有することを特徴とする脂溶性ビタミン含有組成物、
２．上記１に記載の脂溶性ビタミン含有組成物を含むことを特徴とする飲食品、
３．炭酸カリウムを脂溶性ビタミンに添加することを特徴とする、脂溶性ビタミン含有組成物の安定化方法、
からなっている。As a result of intensive studies to solve the above problems, the present inventors have found that the above problems can be solved by adding potassium carbonate to fat-soluble vitamins. The present inventor has further studied based on these findings and has completed the present invention.
That is, the present invention
1. A fat-soluble vitamin-containing composition characterized by containing a fat-soluble vitamin and potassium carbonate;
2. A food or drink comprising the fat-soluble vitamin-containing composition according to 1 above,
3. A method for stabilizing a fat-soluble vitamin-containing composition, comprising adding potassium carbonate to a fat-soluble vitamin,
It is made up of.
より具体的には、脂溶性ビタミン１００質量部に対して炭酸カリウムを０．００５〜３０質量部含有する脂溶性ビタミン含有組成物、並びに当該脂溶性ビタミン含有組成物を含む飲食品であり、また、脂溶性ビタミン１００質量部に対して炭酸カリウムを０．００５〜３０質量部添加することからなる脂溶性ビタミン含有組成物の安定化方法である。 More specifically, it is a fat-soluble vitamin-containing composition containing 0.005 to 30 parts by mass of potassium carbonate with respect to 100 parts by mass of the fat-soluble vitamin, and a food or drink containing the fat-soluble vitamin-containing composition. This is a method for stabilizing a fat-soluble vitamin-containing composition comprising adding 0.005 to 30 parts by mass of potassium carbonate to 100 parts by mass of a fat-soluble vitamin.
本発明の脂溶性ビタミン含有組成物は安定であり、脂溶性ビタミンの含量低下が抑制され、品質上の経時変化が少なく、さらに脂溶性ビタミン含有組成物を飲食品に配合しても脂溶性ビタミンの含量低下が抑制される。 The fat-soluble vitamin-containing composition of the present invention is stable, suppresses a decrease in the content of the fat-soluble vitamin, has little change over time in quality, and even if the fat-soluble vitamin-containing composition is added to food and drink, Reduction in the content of is suppressed.
本発明で用いられる脂溶性ビタミンとしては、例えば、ビタミンＡ、ビタミンＥ、ビタミンＤ、ビタミンＫ、ユビキノンなどやこれらの誘導体が挙げられる。脂溶性ビタミンの具体例としては、例えば、レチノール、３−デヒドロレチノール、レチナール、３−デヒドロレチナール、レチノイン酸、３−デヒドロレチノイン酸およびこれらの酢酸エステルまたはパルミチン酸エステルなどの誘導体などのビタミンＡ類；エルゴカルシフェノール、コレカルシフェノールおよびこれらの硫酸エステルなどの誘導体などのビタミンＤ類；α−トコフェロール、β−トコフェロール、γ−トコフェロール、δ−トコフェロール、α−トコトリエノール、β−トコトリエノール、γ−トコトリエノール、δ−トコトリエノールおよびこれらの酢酸エステルまたはニコチン酸エステルもしくはリン酸エステルなどの誘導体などのビタミンＥ類；フィロキノン、メナキノン、メナジオンなどのビタミンＫ類；ユビキノンの有するイソプレン残基数が１〜１２のユビキノン；などをそれぞれ挙げることができる。これらの脂溶性ビタミンは、一種または二種以上を組み合わせることができる。 Examples of the fat-soluble vitamin used in the present invention include vitamin A, vitamin E, vitamin D, vitamin K, ubiquinone, and derivatives thereof. Specific examples of the fat-soluble vitamin include, for example, vitamins A such as retinol, 3-dehydroretinol, retinal, 3-dehydroretinal, retinoic acid, 3-dehydroretinoic acid and derivatives thereof such as acetate or palmitate thereof. Vitamin D such as ergocalciphenol, cholecalciphenol and derivatives thereof such as sulfate; α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, α-tocotrienol, β-tocotrienol, γ-tocotrienol, Vitamin E such as δ-tocotrienol and derivatives thereof such as acetate, nicotinate or phosphate; vitamin K such as phylloquinone, menaquinone, menadione; ubiquinone And ubiquinone having 1 to 12 isoprene residues. These fat-soluble vitamins can be used alone or in combination of two or more.
本発明で用いられる脂溶性ビタミンは、天然物より公知の方法で抽出精製して得たもの、また、公知の方法で化学合成して得たもの、さらには、微生物などを用いる発酵法によって得た何れのものであってもよい。 The fat-soluble vitamin used in the present invention is obtained by extraction and purification from a natural product by a known method, obtained by chemical synthesis by a known method, and further obtained by fermentation using a microorganism or the like. Any of these may be used.
本発明で用いられる脂溶性ビタミンは、脂溶性ビタミンの濃度を調整するために食用油脂を加えたものであっても良い。前記食用油脂としては、食用可能な油脂であれば特に限定はないが、例えば、サフラワー油、ぶどう油、大豆油、ひまわり油、小麦胚芽油、とうもろこし油、綿実油、ごま油、なたね油、こめ油、落花生油、オリーブ油、中鎖脂肪酸トリグリセリド（ＭＣＴ）、パーム油、パーム核油、やし油などの植物油脂や、牛脂、ラード、乳脂及び魚油などの動物油脂、さらにこれらの動植物油脂を水素添加あるいはエステル交換した油脂などが挙げられる。上記食用油脂は、一種または二種以上を組み合わせることができる。
また、脂溶性ビタミンが天然物より抽出精製して得たものである場合、抽出原料由来の食用油脂を含むものであっても良い。The fat-soluble vitamins used in the present invention may be those added with edible fats and oils in order to adjust the concentration of the fat-soluble vitamins. The edible fats and oils are not particularly limited as long as they are edible fats and oils, for example, safflower oil, grape oil, soybean oil, sunflower oil, wheat germ oil, corn oil, cottonseed oil, sesame oil, rapeseed oil, rice oil, Hydrogenation of vegetable oils such as peanut oil, olive oil, medium chain fatty acid triglyceride (MCT), palm oil, palm kernel oil, palm oil, animal fats such as beef tallow, lard, milk fat and fish oil, and these animal and vegetable oils Examples include transesterified oils and fats. The said edible fats and oils can combine 1 type, or 2 or more types.
Moreover, when the fat-soluble vitamin is obtained by extraction and purification from a natural product, it may contain edible fats and oils derived from the extraction raw material.
本発明で用いられる炭酸カリウムは、形状や純度など特に制限はなく一般に市販されているものを用いることができる。炭酸カリウムの好ましい形態としては、脂溶性ビタミンとの接触面積が大きくなる形態である。 The potassium carbonate used in the present invention is not particularly limited in terms of shape and purity, and commercially available products can be used. A preferred form of potassium carbonate is a form that increases the contact area with the fat-soluble vitamin.
本発明で用いられる炭酸カリウムの添加量は、脂溶性ビタミン１００質量部に対して炭酸カリウムを約０．００５〜３０質量部添加することが好ましく、０．０１〜１０質量部がさらに好ましい。脂溶性ビタミン１００質量部に対して炭酸カリウムの添加量が約０．００５質量部より少ないと、脂溶性ビタミンの安定化効果を十分に発揮することができない場合があり、炭酸カリウム添加量が約３０質量部より多いと脂溶性ビタミンの安定性効果がそれ以上向上しない場合がある。 The amount of potassium carbonate used in the present invention is preferably about 0.005 to 30 parts by mass, more preferably 0.01 to 10 parts by mass with respect to 100 parts by mass of the fat-soluble vitamin. If the amount of potassium carbonate added is less than about 0.005 parts by mass with respect to 100 parts by mass of the fat-soluble vitamin, the stabilizing effect of the fat-soluble vitamin may not be fully exhibited, and the amount of potassium carbonate added is about When the amount is more than 30 parts by mass, the stability effect of the fat-soluble vitamin may not be further improved.
本発明の脂溶性ビタミン含有組成物には、所望により公知の酸化防止剤を併用することができる。酸化防止剤としては、例えば、ビタミンＣ及びその誘導体、エリソルビン酸ナトリウムなどの水溶性成分、カテキン、没食子酸、ヤマモモ抽出物、ルチン、ケルセチン配糖体、ミリスチン、イソクエルセチン、ナリンゲニンなどのフラボノイド類、ケンフェロールなどのカルコン類及びこれらの誘導体類などを挙げられる。 A known antioxidant can be used in combination with the fat-soluble vitamin-containing composition of the present invention as desired. Antioxidants include, for example, vitamin C and its derivatives, water-soluble components such as sodium erythorbate, catechin, gallic acid, bayberry extract, rutin, quercetin glycoside, myristin, isoquercetin, naringenin and other flavonoids, Examples thereof include chalcones such as kaempferol and derivatives thereof.
本発明の脂溶性ビタミン含有組成物は、脂溶性ビタミンに炭酸カリウムが接触するように含有すればよく、脂溶性ビタミン含有組成物の形態は特に制限はないが、例えば脂溶性ビタミン中に炭酸カリウムが分散した脂溶性ビタミン含有組成物、脂溶性ビタミンと炭酸カリウムとを含有する乳化溶液とした水中油型乳化組成物状の脂溶性ビタミン含有組成物、水中油型乳化組成物状の脂溶性ビタミン含有組成物を乾燥した粉末状の脂溶性ビタミン含有組成物、粉末状の脂溶性ビタミン含有組成物を造粒した顆粒状の脂溶性ビタミン含有組成物、脂溶性ビタミン含有組成物を芯物質としたマイクロカプセル状の脂溶性ビタミン含有組成物などが挙げられる。
以下に脂溶性ビタミン含有組成物、水中油型乳化組成物状の脂溶性ビタミン含有組成物及び粉末状の脂溶性ビタミン含有組成物の製造方法について例示する。The fat-soluble vitamin-containing composition of the present invention may be contained so that potassium carbonate comes into contact with the fat-soluble vitamin, and the form of the fat-soluble vitamin-containing composition is not particularly limited. For example, potassium carbonate is contained in the fat-soluble vitamin. Oil-soluble vitamin-containing composition in which oil is dispersed, a fat-soluble vitamin-containing composition in the form of an oil-in-water emulsion composition in an emulsion solution containing a fat-soluble vitamin and potassium carbonate, and a fat-soluble vitamin in the form of an oil-in-water emulsion composition Powdered fat-soluble vitamin-containing composition obtained by drying the contained composition, granular fat-soluble vitamin-containing composition obtained by granulating the powdered fat-soluble vitamin-containing composition, and fat-soluble vitamin-containing composition as a core substance Examples include a microcapsule-like fat-soluble vitamin-containing composition.
Examples of the method for producing a fat-soluble vitamin-containing composition, a fat-soluble vitamin-containing composition in the form of an oil-in-water emulsion composition, and a powdery fat-soluble vitamin-containing composition are illustrated below.
脂溶性ビタミン含有組成物の製造方法は特に制限はなく、例えば脂溶性ビタミンに炭酸カリウムを攪拌しながら加えて分散することにより製造することができる。
脂溶性ビタミン含有組成物の製造方法の具体例としては、脂溶性ビタミンに所望により油脂を加えて脂溶性ビタミン含量を調製した後、約１００℃以下、好ましくは約６０〜８０℃に加熱し、炭酸カリウムを加えて攪拌し均一に分散させ、室温まで冷却して脂溶性ビタミン含有組成物を製造することができる。The method for producing the fat-soluble vitamin-containing composition is not particularly limited. For example, the fat-soluble vitamin-containing composition can be produced by adding potassium carbonate to a fat-soluble vitamin while stirring.
As a specific example of the method for producing a fat-soluble vitamin-containing composition, fat and fat are optionally added to the fat-soluble vitamin to prepare a fat-soluble vitamin content, and then heated to about 100 ° C. or less, preferably about 60 to 80 ° C., Potassium carbonate can be added, stirred and dispersed uniformly, and cooled to room temperature to produce a fat-soluble vitamin-containing composition.
水中油型乳化組成物状の脂溶性ビタミン含有組成物の製造方法は特に制限はなく、公知の方法で乳化することにより製造することができる。
水中油型乳化組成物状の脂溶性ビタミン含有組成物の製造方法の具体例としては、水、糖類、多価アルコール、増粘安定剤、乳化剤、および炭酸カリウムを加えて攪拌しながら約４０〜８０℃、好ましくは約６０〜８０℃に加熱し、約１００℃以下、好ましくは約６０〜８０℃に保持した脂溶性ビタミンをゆっくり加えながら高速回転式分散・乳化機を用いて回転数約４０００〜２００００ｒｐｍで攪拌時間約１０〜６０分間乳化することにより、水中油型乳化組成物状の脂溶性ビタミン含有組成物を製造することができる。There is no restriction | limiting in particular in the manufacturing method of the fat-soluble vitamin containing composition of an oil-in-water-type emulsion composition, It can manufacture by emulsifying by a well-known method.
Specific examples of the method for producing a fat-soluble vitamin-containing composition in the form of an oil-in-water emulsified composition include water, sugars, polyhydric alcohols, thickening stabilizers, emulsifiers, and potassium carbonate with stirring and about 40- Heat to 80 ° C., preferably about 60-80 ° C., and slowly add fat-soluble vitamins kept at about 100 ° C. or less, preferably about 60-80 ° C., using a high-speed rotary disperser / emulsifier and a rotational speed of about 4000 A fat-soluble vitamin-containing composition in the form of an oil-in-water emulsion composition can be produced by emulsifying at about 20,000 rpm with a stirring time of about 10 to 60 minutes.
水中油型乳化組成物状の脂溶性ビタミン含有組成物を製造するための装置としては特に制限はなく、例えば、攪拌機、加熱用のジャケットおよび邪魔板などを備えた通常の攪拌・混合槽を用いることができる。装備する攪拌機としては、ＴＫホモミクサー（プライミクス社製）またはクレアミックス（エムテクニック社製）などの高速回転式分散・乳化機が好ましく用いられる。また、これらの装置で乳化した液を、高圧式均質化処理機を使用して、さらに均質化してもよい。ここで高圧式均質化処理機としては、例えばＡＰＶゴーリンホモジナイザー（ＡＰＶ社製）、マイクロフルイダイザー（マイクロフルイデックス社製）、アルティマイザー（スギノマシン社製）またはナノマイザー（大和製罐社製）などを好ましく使用することができる。上記高圧式均質化処理機に代えて、例えば超音波乳化機などの均質化処理機を用いてもよい。 There is no particular limitation on the apparatus for producing the fat-soluble vitamin-containing composition in the form of an oil-in-water emulsion composition. For example, a normal stirring / mixing tank equipped with a stirrer, a heating jacket, a baffle plate, and the like is used. be able to. As a stirrer to be equipped, a high-speed rotary dispersing / emulsifying machine such as TK homomixer (manufactured by PRIMIX Co., Ltd.) or CLEARMIX (manufactured by M Technique Co., Ltd.) is preferably used. Moreover, you may further homogenize the liquid emulsified with these apparatuses using a high-pressure-type homogenizer. Here, examples of the high-pressure homogenizer include an APV gorin homogenizer (manufactured by APV), a microfluidizer (manufactured by Microfluidics), an optimizer (manufactured by Sugino Machine), or a nanomizer (manufactured by Daiwa Steel). Can be preferably used. Instead of the high-pressure homogenizer, a homogenizer such as an ultrasonic emulsifier may be used.
水中油型乳化組成物状の脂溶性ビタミン含有組成物に用いられる糖類としては、例えば、澱粉分解物、異性化液糖、オクテニルコハク酸エステル化澱粉などが挙げられ、上記した糖類は一種または二種以上を組み合わせることができる。 Examples of the saccharide used in the oil-in-water emulsified composition-containing fat-soluble vitamin-containing composition include, for example, starch degradation products, isomerized liquid sugar, octenyl succinate-starch, and the like. The above can be combined.
水中油型乳化組成物状の脂溶性ビタミン含有組成物に用いられる多価アルコールとしては、例えば、グリセリン、Ｄ−ソルビトール、還元澱粉分解物などが挙げられ、上記した多価アルコールは一種または二種以上を組み合わせることができる。 Examples of the polyhydric alcohol used in the oil-in-water emulsion composition-containing fat-soluble vitamin-containing composition include glycerin, D-sorbitol, reduced starch degradation products, and the like. The above can be combined.
水中油型乳化組成物状の脂溶性ビタミン含有組成物に用いられる増粘安定剤としては、例えば、寒天、カラギーナン、ファーセレラン、アルギン酸塩、ローカストビーンガム、グアーガム、タマリンドガム、サイリウムシードガム、アラビアガム、トラガントガム、カラヤガム、ペクチン、アラビノガラクタン、プルラン、デキストラン、カードラン、セルロース、ヘミセルロース、キサンタンガム、マンナンなどが挙げられ、上記した増粘安定剤は一種または二種以上を組み合わせることができる。 Examples of the thickening stabilizer used in a fat-soluble vitamin-containing composition in the form of an oil-in-water emulsion composition include, for example, agar, carrageenan, farseleran, alginate, locust bean gum, guar gum, tamarind gum, psyllium gum, and gum arabic. , Tragacanth gum, karaya gum, pectin, arabinogalactan, pullulan, dextran, curdlan, cellulose, hemicellulose, xanthan gum, mannan and the like, and the above thickening stabilizers can be used alone or in combination of two or more.
水中油型乳化組成物状の脂溶性ビタミン含有組成物に用いられる乳化剤としては、例えば、グリセリン脂肪酸エステル、グリセリン有機酸脂肪酸エステル、ポリグリセリン脂肪酸エステル、ポリグリセリン縮合リシノール酸エステル、ショ糖脂肪酸エステル、ソルビタン脂肪酸エステル、ポリオキシエチレンソルビタン脂肪酸エステル、プロピレングリコール脂肪酸エステル、レシチン、サポニンなどが挙げられ、これらの一種又は二種以上を用いることができる。 Examples of the emulsifier used in the oil-in-water emulsion composition-containing fat-soluble vitamin-containing composition include, for example, glycerin fatty acid ester, glycerin organic acid fatty acid ester, polyglycerin fatty acid ester, polyglycerin condensed ricinoleic acid ester, sucrose fatty acid ester, Examples include sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, propylene glycol fatty acid ester, lecithin, saponin, and the like, and one or more of these can be used.
粉末状の脂溶性ビタミン含有組成物の製造方法は特に制限はなく、水中油型乳化組成物状の脂溶性ビタミン含有組成物、または、水中油型乳化組成物状の脂溶性ビタミン含有組成物に粉末化基材などを加えたものを公知の方法で乾燥することにより製造することができる。 The production method of the powdery fat-soluble vitamin-containing composition is not particularly limited, and the oil-soluble vitamin-containing composition in the form of an oil-in-water emulsion composition or the fat-soluble vitamin-containing composition in the form of an oil-in-water emulsion composition It can manufacture by drying what added the powdered base material etc. by a well-known method.
粉末状の脂溶性ビタミン含有組成物の製造方法の具体例としては、水に粉末化基材、増粘安定剤、炭酸カリウムおよび乳化剤を加えて攪拌しながら約４０〜８０℃、好ましくは約６０〜８０℃に加熱し、約１００℃以下、好ましくは約６０〜８０℃に保持した脂溶性ビタミンをゆっくり加えながら高速回転式分散・乳化機を用いて回転数約４０００〜２００００ｒｐｍで攪拌時間約１０〜６０分間乳化して脂溶性ビタミンを含有する乳化液を得る。次に脂溶性ビタミンを含有する乳化液を乾燥し粉末化することにより、粉末状の脂溶性ビタミン含有組成物を製造することができる。 As a specific example of the method for producing a powdery fat-soluble vitamin-containing composition, a powdered base material, a thickening stabilizer, potassium carbonate and an emulsifier are added to water and stirred at about 40 to 80 ° C., preferably about 60 Heat to -80 ° C and slowly add fat-soluble vitamins kept at about 100 ° C or less, preferably about 60-80 ° C, while using a high-speed rotary dispersion / emulsifier at a rotational speed of about 4000-20000 rpm and a stirring time of about 10 Emulsify for 60 minutes to obtain a fat-soluble vitamin. Next, a powdery fat-soluble vitamin-containing composition can be produced by drying and pulverizing the emulsion containing the fat-soluble vitamin.
上記乾燥方法としては、例えば、噴霧乾燥、ドラム乾燥、ベルト乾燥、真空乾燥あるいは真空凍結乾燥などが挙げられるが、好ましくは噴霧乾燥である。噴霧乾燥装置に特に制限はなく、噴射式噴霧乾燥装置または回転円盤式噴霧乾燥装置など公知の装置を使用することができる。また、噴霧乾燥の操作条件に特に制限は無く、例えば、脂溶性ビタミンと炭酸カリウムを含有する乳化液を加圧ノズル式噴霧乾燥装置に供給し、熱風入口温度約１５０〜２７０℃、排気温度約７０〜１３０℃の条件下で噴霧乾燥し、乾燥物をサイクロンで捕集することにより、粉末状の脂溶性ビタミン含有組成物を得る。得られる粉末の平均粒子径は約２０〜２００μｍ、好ましくは約５０〜１００μｍである。また得られる粉末の乾燥減量は約１０質量％以下、好ましくは約７質量％以下、更に好ましくは約５質量％以下である。 Examples of the drying method include spray drying, drum drying, belt drying, vacuum drying, and vacuum freeze drying, and spray drying is preferable. There is no restriction | limiting in particular in a spray-drying apparatus, Well-known apparatuses, such as a spray-type spray-drying apparatus or a rotary disk type spray-drying apparatus, can be used. There are no particular restrictions on the operating conditions for spray drying. For example, an emulsion containing a fat-soluble vitamin and potassium carbonate is supplied to a pressure nozzle spray drying apparatus, the hot air inlet temperature is about 150 to 270 ° C., and the exhaust temperature is about It spray-drys on 70-130 degreeC conditions, A powdery fat-soluble vitamin containing composition is obtained by collecting a dried material with a cyclone. The average particle diameter of the obtained powder is about 20 to 200 μm, preferably about 50 to 100 μm. The loss on drying of the obtained powder is about 10% by mass or less, preferably about 7% by mass or less, more preferably about 5% by mass or less.
粉末状の脂溶性ビタミン含有組成物に用いられる粉末化基材としては、サイクロデキストリン、デキストリン、乳糖、カゼイン及びその塩類、澱粉、化工澱粉、ブドウ糖、果糖などの単糖、ショ糖、乳糖、麦芽糖などの二糖、デキストリン、粉末水飴などのでん粉分解物、マルトトリオース、マルトテトラオース、マルトペンタオース、マルトヘキサオースなどのマルトオリゴ糖類、ソルビトール、マンニトール、マルチトール、粉末還元水飴、粉末還元パラチノースなどの糖アルコール類などが挙げられ、更に好ましくはサイクロデキストリンが挙げられ、上記した粉末化基材は１種又は２種以上を組み合わせて用いることができる。 Powdered base materials used in powdered fat-soluble vitamin-containing compositions include cyclodextrins, dextrins, lactose, casein and its salts, starch, modified starch, glucose, fructose and other simple sugars, sucrose, lactose, maltose Such as disaccharides, dextrin, starch starch such as powdered starch syrup, malto-oligosaccharides such as maltotriose, maltotetraose, maltopentaose, maltohexaose, sorbitol, mannitol, maltitol, powdered reduced starch syrup, powdered reduced palatinose, etc. Sugar alcohols, and the like, more preferably cyclodextrins, and the above powdered base materials can be used alone or in combination of two or more.
上記サイクロデキストリンとしては、環状オリゴ糖で、グルコース数の違いによってα、β、γの構造をもつサイクロデキストリンや、それらに低級アルキル基を導入したメチルサイクロデキストリン、エチルサイクロデキストリン、ヒドロキシアルキル化したヒドロキシメチルサイクロデキストリン、ヒドロキシエチルサイクロデキストリン、ヒドロキシプロピルサイクロデキストリン、ヒドロキシブチルサイクロデキストリンなどが挙げられ、好ましくはβ−サイクロデキストリン、γ−サイクロデキストリンが挙げられる。 Cyclodextrins are cyclic oligosaccharides that have α, β, and γ structures depending on the number of glucose, methyl cyclodextrins, ethyl cyclodextrins, and hydroxyalkylated hydroxys into which lower alkyl groups have been introduced. Examples thereof include methyl cyclodextrin, hydroxyethyl cyclodextrin, hydroxypropyl cyclodextrin, hydroxybutyl cyclodextrin and the like, preferably β-cyclodextrin and γ-cyclodextrin.
粉末状の脂溶性ビタミン含有組成物には、本発明の目的・効果を阻害しない範囲で、例えば流動性付与物質を加えることができる。流動性付与物質としては、例えば、リン酸三カルシウム、微粒二酸化ケイ素などが挙げられる。 For example, a fluidity-imparting substance can be added to the powdery fat-soluble vitamin-containing composition as long as the object and effect of the present invention are not impaired. Examples of the fluidity-imparting substance include tricalcium phosphate and fine silicon dioxide.
斯くして得られた各様態の脂溶性ビタミン含有組成物は、従来の脂溶性ビタミン、及び従来の脂溶性ビタミン組成物と同じ様に飲食品、医薬品、化粧品などに用いることができる。
本発明の脂溶性ビタミンは安定性に優れているため、飲食品、医薬品、化粧品などに配合した場合においても安定性は保持される。
本発明の脂溶性ビタミン含有組成物の飲食品、医薬品、化粧品などへの配合量は特に制限されず、通常の配合量と同量とすることができる。The fat-soluble vitamin-containing composition of each aspect thus obtained can be used for foods and drinks, pharmaceuticals, cosmetics and the like in the same manner as conventional fat-soluble vitamins and conventional fat-soluble vitamin compositions.
Since the fat-soluble vitamin of the present invention is excellent in stability, stability is maintained even when blended in foods, drinks, pharmaceuticals, cosmetics and the like.
The blending amount of the fat-soluble vitamin-containing composition of the present invention in foods and drinks, pharmaceuticals, cosmetics and the like is not particularly limited, and can be the same as the usual blending amount.
脂溶性ビタミン含有組成物を添加する飲食品としては、例えば、脂溶性ビタミンを強化する目的で加える育児用粉乳、機能性飲料、スポーツ飲料、栄養補給飲料などが挙げられる。
脂溶性ビタミン含有組成物を添加する医薬品としては、例えば、点眼薬、点滴などが挙げられ、脂溶性ビタミン含有組成物を添加する化粧品としては、例えば、美肌剤、美毛剤、育毛剤などが挙げられる。Examples of the foods and drinks to which the fat-soluble vitamin-containing composition is added include infant formula for adding fat-soluble vitamins, functional drinks, sports drinks, and nutritional supplement drinks.
Examples of pharmaceuticals to which a fat-soluble vitamin-containing composition is added include eye drops, infusions, and the like, and cosmetics to which a fat-soluble vitamin-containing composition is added include, for example, skin beautifying agents, hairdressing agents, hair restorers, and the like. Can be mentioned.
以下に本発明を実施例で説明するが、これは本発明を単に説明するだけのものであって、本発明を限定するものではない。 The present invention will now be described by way of examples, which are merely illustrative of the invention and do not limit the invention.
［脂溶性ビタミン含有組成物］
（１）原材料
（ａ）脂溶性ビタミン
ビタミンＥ（製品名：理研Ｅオイル７０５；理研ビタミン社製、総トコフェロール含量６８質量％以上、ｄ−α−トコフェロール含量３５％）
ビタミンＡ（製品名：ビタミンＡ脂肪酸エステル；ＢＡＳＦジャパン社製、ビタミンＡパルミテート含量１７０万Ｉ．Ｕ．／ｇ）
ビタミンＤ（製品名：理研Ｄ３オイル１００；理研ビタミン社製、ビタミンＤ３含量１０万Ｉ．Ｕ．／ｇ）
（ｂ）炭酸塩
炭酸カリウム（製品名：炭酸カリウム；和光純薬工業社製）
炭酸ナトリウム（製品名：炭酸ナトリウム；純正化学社製）
炭酸カルシウム（製品名：炭酸カルシウム；和光純薬工業社製）[Fat-soluble vitamin-containing composition]
(1) Raw material (a) Fat-soluble vitamin Vitamin E (Product name: Riken E Oil 705; manufactured by Riken Vitamin Co., Ltd., total tocopherol content 68% by mass or more, d-α-tocopherol content 35%)
Vitamin A (Product name: Vitamin A fatty acid ester; manufactured by BASF Japan, Vitamin A palmitate content 1.7 million IU / g)
Vitamin D (Product name: Riken D3 Oil 100; manufactured by Riken Vitamin Co., Ltd., vitamin D3 content 100,000 IU / g)
(B) Potassium carbonate carbonate (Product name: Potassium carbonate; Wako Pure Chemical Industries, Ltd.)
Sodium carbonate (Product name: Sodium carbonate; manufactured by Junsei Kagaku)
Calcium carbonate (Product name: Calcium carbonate; Wako Pure Chemical Industries, Ltd.)
（２）脂溶性ビタミン含有組成物の配合
上記原材料を用いて作製した脂溶性ビタミン含有組成物の配合を表１に示した。(2) Composition of fat-soluble vitamin-containing composition Table 1 shows the composition of the fat-soluble vitamin-containing composition prepared using the above raw materials.
表中の数値は、質量（ｇ）である。 The numerical value in a table | surface is mass (g).
（３）脂溶性ビタミン含有組成物の作製方法
表１に示した配合に基づいて各原材料を混合し脂溶性ビタミン含有組成物（実施例品１〜６、比較例品１〜５）を作製した。尚、混合は５００ｍＬビーカーに各原材料を入れ、約６０℃に加熱し、攪拌機（製品名：スリーワンモーター、形式：ＦＢＬ−６００；ＨＥＩＤＯＮ社製）を用いて、約３００ｒｐｍで１５分間攪拌して行った。(3) Preparation method of fat-soluble vitamin-containing composition Each raw material was mixed based on the formulation shown in Table 1 to prepare fat-soluble vitamin-containing compositions (Example products 1-6, Comparative products 1-5). . Mixing is performed by putting each raw material in a 500 mL beaker, heating to about 60 ° C., and stirring for 15 minutes at about 300 rpm using a stirrer (product name: Three-One Motor, Model: FBL-600; manufactured by HEIDON). It was.
（４）脂溶性ビタミン含有組成物の安定性試験方法及び結果
得られた脂溶性ビタミン含有組成物（実施例品１〜６、比較例品１〜５）各１０ｇをガラスシャーレにとり、５５℃の暗所で保管した。ビタミンＥを用いた含有組成物の保管期間は３０日間、７０日間とし、ビタミンＡ、ビタミンＤを使用した脂溶性ビタミン含有組成物の保管期間は４日間、８日間、１２日間とした。
脂溶性ビタミン含有組成物中に含まれる脂溶性ビタミン含量を測定し、作製直後の脂溶性ビタミンを１００質量％として保管後の脂溶性ビタミンの含有質量％（残存率）を計算した。結果を表２に示す。(4) Stability test method and results of fat-soluble vitamin-containing composition 10 g of each of the obtained fat-soluble vitamin-containing compositions (Example products 1 to 6, Comparative products 1 to 5) was placed in a glass petri dish at 55 ° C. Stored in the dark. The storage period of the composition containing vitamin E was 30 days and 70 days, and the storage period of the fat-soluble vitamin-containing composition using vitamin A and vitamin D was 4, 8, and 12 days.
The fat-soluble vitamin content contained in the fat-soluble vitamin-containing composition was measured, and the fat-soluble vitamin content mass (residual rate) after storage was calculated with the fat-soluble vitamin immediately after production as 100 mass%. The results are shown in Table 2.
ここで、ビタミンＥ、ビタミンＡ、ビタミンＤの測定方法を以下に示す。
ビタミンＥの測定方法
食品添加物公定書解説書（第８版）のｄ−α−トコフェロールの測定方法に記載（Ｄ−１１８３〜１１８４）の定量方法で検液及び標準液を調整した。この検液及び標準液について高速液体クロマトグラフィを用いて下記条件で測定し、総トコフェロール（ビタミンＥ）の含量を求めた。
検出器：紫外線吸光光度計（製品名：ＳＰＤ−１０ＡＶ；島津製作所社製）
検出波長：２９２ｎｍ
カラム：Ｆｉｎｅｐａｋ ＳＩＬ Ｃ１８Ｔ−５ φ４．６×２５ｃｍ（日本分光社製）
移動相：ヘキサン：２−プロパノール混液＝９８：２
流量：１．０ｍＬ／ｍｉｎ
カラム温度：４０℃
注入量：２０μＬHere, methods for measuring vitamin E, vitamin A, and vitamin D are shown below.
Method for Measuring Vitamin E The test solution and the standard solution were prepared by the quantitative method described in (D-1183 to 1184) described in the method for measuring d-α-tocopherol in the Food Additives Official Manual (8th edition). About this test solution and a standard solution, it measured on the following conditions using the high performance liquid chromatography, and calculated | required the content of total tocopherol (vitamin E).
Detector: UV absorption photometer (Product name: SPD-10AV; manufactured by Shimadzu Corporation)
Detection wavelength: 292 nm
Column: Finepak SIL C18T-5 φ4.6 × 25 cm (manufactured by JASCO)
Mobile phase: hexane: 2-propanol mixed solution = 98: 2
Flow rate: 1.0 mL / min
Column temperature: 40 ° C
Injection volume: 20 μL
ビタミンＡの測定方法
食品添加物公定書解説書（第８版）のビタミンＡ脂肪酸エステルの測定方法に記載（Ｄ−１３３９）の定量方法で測定してビタミンＡの含量を求めた。その際吸光度測定は、紫外分光光度計（型式：Ｕ−３３１０；日立製作所社製）を用いて行った。Vitamin A measurement method Vitamin A content was determined by the quantitative method described in (D-1339) described in the measurement method of vitamin A fatty acid ester in the Food Additives Official Manual (8th edition). At that time, the absorbance was measured using an ultraviolet spectrophotometer (model: U-3310; manufactured by Hitachi, Ltd.).
ビタミンＤの測定方法
本発明の脂溶性ビタミン含有組成物（ビタミンＤ含有品）のビタミンＤとして約２５００Ｉ．Ｕ．に対応する量を精密に量り、共栓付き褐色試験管に入れ、ブチルヒドロキシトルエン約５ｍｇ、ｎ−ヘキサン５ｍＬを加え、４０〜６０℃の水浴中でｎ−ヘキサンを完全に除去して検液とした。
ビタミンＤ３の標準原液（日本薬局方適合品）約３０ｍｇを１００ｍＬ褐色メスフラスコに精密に量り入れ、ブチルヒドロキシトルエン約５ｍｇを加えてｎ−ヘキサンでメスアップする。ここから正確に５ｍＬを量り５０ｍＬ容褐色メスフラスコに入れてｎ−ヘキサンでメスアップした。さらにここから正確に１０ｍＬを量り、５０ｍＬ容褐色メスフラスコに入れてｎ−ヘキサンでメスアップして標準液とした。
得られた検液及び標準液を、高速液体クロマトグラフィを用いて下記条件で測定し、標準品の分析結果（ピーク面積）をもとにビタミンＤの含量を求めた。
検出器：紫外吸光光度計（製品名：ＳＰＤ−１０ＡＶ；島津製作所社製）
検出波長：２６５ｎｍ
カラム：Ｒｅｓｏｌｖｅ ｓｉｌｉｃａ ５μｍ：φ３．９×１５０ｍｍ（Ｗａｔｅｒｓ社製）
移動相：ヘキサン：２−プロパノール混液＝９５：５
流量：０．５ｍＬ／ｍｉｎ
カラム温度：３０℃
注入量：５０μＬMethod for Measuring Vitamin D As the vitamin D of the fat-soluble vitamin-containing composition (vitamin D-containing product) of the present invention, about 2500 I.D. U. Precisely measure the amount corresponding to, put in a brown test tube with a stopper, add about 5 mg of butylhydroxytoluene and 5 mL of n-hexane, completely remove n-hexane in a water bath at 40-60 ° C. It was.
About 30 mg of vitamin D3 standard stock solution (Japanese Pharmacopoeia compatible product) is accurately weighed into a 100 mL brown volumetric flask, added with about 5 mg of butylhydroxytoluene and made up with n-hexane. From here, 5 mL was accurately weighed and placed in a 50 mL brown volumetric flask and diluted with n-hexane. Furthermore, 10 mL was measured accurately from here, put into a 50 mL brown volumetric flask and made up with n-hexane to obtain a standard solution.
The obtained test solution and standard solution were measured under the following conditions using high performance liquid chromatography, and the content of vitamin D was determined based on the analysis result (peak area) of the standard product.
Detector: UV absorption photometer (Product name: SPD-10AV; manufactured by Shimadzu Corporation)
Detection wavelength: 265 nm
Column: Resolve silica 5 μm: φ3.9 × 150 mm (manufactured by Waters)
Mobile phase: hexane: 2-propanol mixed solution = 95: 5
Flow rate: 0.5mL / min
Column temperature: 30 ° C
Injection volume: 50 μL
表中の数値は、作成直後の脂溶性ビタミンを１００質量％として保管後の脂溶性ビタミンの各含有質量％（残存率）である。 The numerical values in the table are each mass% (residual rate) of the fat-soluble vitamins after storage, with the fat-soluble vitamins immediately after creation as 100 mass%.
表中の結果より判明するように、実施例である炭酸カリウムを含有する脂溶性ビタミン含有組成物は、脂溶性ビタミンの残存率が高く、長期間の保管でも良好な結果であった。一方、炭酸カリウムを含有しない比較例品は、脂溶性ビタミンの残存率が低く、安定性は良くない結果であった。 As can be seen from the results in the table, the fat-soluble vitamin-containing composition containing potassium carbonate as an example had a high residual ratio of fat-soluble vitamins, and was a good result even after long-term storage. On the other hand, the comparative product containing no potassium carbonate had a low residual ratio of fat-soluble vitamins and had poor stability.
［粉末状の脂溶性ビタミン含有組成物］
（１）原材料
ビタミンＡ（製品名：ビタミンＡ脂肪酸エステル、ＢＡＳＦジャパン社製 ビタミンＡパルミテート含量１７０万Ｉ．Ｕ．／ｇ）
β−サイクロデキストリン（商品名：ＣＡＶＡＭＡＸ（Ｒ） Ｗ７ Ｆｏｏｄ；シクロケム社製）
デキストリン（商品名：パインデックス＃２；松谷化学工業社製）
アラビアガム（商品名：アラビックコールＳＳ；三栄薬品貿易社製）
炭酸カリウム（製品名：炭酸カリウム；和光純薬工業社製）[Powdered fat-soluble vitamin-containing composition]
(1) Raw material Vitamin A (Product name: Vitamin A fatty acid ester, manufactured by BASF Japan Ltd. Vitamin A palmitate content 1.7 million IU / g)
β-cyclodextrin (trade name: CAVAMAX® W7 Food; manufactured by Cyclochem)
Dextrin (trade name: Paindex # 2; manufactured by Matsutani Chemical Industry Co., Ltd.)
Gum arabic (trade name: Arabic Coal SS; manufactured by Sanei Pharmaceutical Trading Co., Ltd.)
Potassium carbonate (Product name: Potassium carbonate; Wako Pure Chemical Industries, Ltd.)
（２）粉末状の脂溶性ビタミン含有組成物の配合
上記原材料を用いて作製した粉末状の脂溶性ビタミン含有組成物の配合を、表３に示した。(2) Formulation of powdery fat-soluble vitamin-containing composition Table 3 shows the formulation of powdered fat-soluble vitamin-containing composition prepared using the above raw materials.
表中の数値は、質量部である。 The numerical value in a table | surface is a mass part.
（３）粉末状の脂溶性ビタミン含有組成物の作製方法
表３に示した配合に基づいて各原材料を下記方法で乳化液とした後乾燥し、粉末状の脂溶性ビタミン含有組成物（実施例品７、８、比較例品６、７）を作製した。
表３に示したビタミンＡ以外の原材料を２Ｌ容ステンレス製ジョッキに加えてゆっくり混合しながら、液温６０℃まで加温し、６０℃を保ちながら１０分間混合した後、ビタミンＡを加えてＴ．Ｋ．ホモキキサー（型式：ＭＡＲＫ ＩＩ型；プライミクス社製）を用いて８０００ｒｐｍで１５分間撹拌し乳化溶液を得た。各乳化溶液の１回の作製量は１５００ｇである。
得られた乳化溶液をスプレードライヤ（型式：Ｌ−８ｉ；大川原化工機社製）を使用して、噴霧乾燥（送風温度１８０℃）し粉末状の脂溶性ビタミン含有組成物（実施例品７、８ 比較例品６、７）を得た。(3) Preparation method of powdery fat-soluble vitamin-containing composition Each raw material is made into an emulsion by the following method based on the formulation shown in Table 3 and then dried to give a powdery fat-soluble vitamin-containing composition (Examples) Products 7 and 8 and Comparative Products 6 and 7) were produced.
Ingredients other than vitamin A shown in Table 3 were added to a 2L stainless steel mug and slowly mixed, heated to a liquid temperature of 60 ° C, mixed for 10 minutes while maintaining 60 ° C, then added with vitamin A and T . K. The mixture was stirred at 8000 rpm for 15 minutes using a homoxer (model: MARK II type; manufactured by Primix) to obtain an emulsified solution. The production amount of each emulsified solution at a time is 1500 g.
The obtained emulsified solution was spray-dried (fan temperature 180 ° C.) using a spray dryer (model: L-8i; manufactured by Okawara Kako Co., Ltd.) and powdered fat-soluble vitamin-containing composition (Example Product 7, 8 Comparative example products 6 and 7) were obtained.
（４）粉末状の脂溶性ビタミン含有組成物の安定性試験方法及び結果
得られた粉末状の脂溶性ビタミン含有組成物（実施例品７、８ 比較例品６、７）の各３００ｇを１Ｌ容のポリ袋に入れ、４０℃暗所で３０日間、６０日間、９０日間保管した。粉末状の脂溶性ビタミン含有組成物中に含まれる脂溶性ビタミン（ビタミンＡ）含量を測定し、作製直後の脂溶性ビタミン含量を１００質量％として保管後の脂溶性ビタミンの含有質量％（残存率）を計算した。結果を表４に示す。(4) Stability test method and result of powdery fat-soluble vitamin-containing composition 1 L of 300 g of each of the obtained powdery fat-soluble vitamin-containing compositions (Example products 7, 8 Comparative products 6, 7) And stored in a dark plastic bag at 40 ° C. for 30 days, 60 days, and 90 days. The fat-soluble vitamin (vitamin A) content contained in the powdered fat-soluble vitamin-containing composition is measured, and the fat-soluble vitamin content immediately after production is 100% by mass. ) Was calculated. The results are shown in Table 4.
表中の数値は、作成直後の脂溶性ビタミンを１００質量％として保管後の脂溶性ビタミンの各含有質量％（残存率）である。 The numerical values in the table are each mass% (residual rate) of the fat-soluble vitamins after storage, with the fat-soluble vitamins immediately after creation as 100 mass%.
表中に示した結果から判明するように、実施例である炭酸カリウムを含有する粉末状の脂溶性ビタミン含有組成物は、脂溶性ビタミンの残存率が高く、長期間の保管でも安定性は良好であった。一方、炭酸カリウムを含有しない比較例品は、脂溶性ビタミンの残存率が低く、安定性は悪い結果であった。 As can be seen from the results shown in the table, the powdered fat-soluble vitamin-containing composition containing potassium carbonate, which is an example, has a high residual ratio of fat-soluble vitamins and good stability even during long-term storage. Met. On the other hand, the comparative product which does not contain potassium carbonate had a low residual rate of fat-soluble vitamins and had poor stability.
［粉末状の脂溶性ビタミン含有組成物を含有する食品］
（１）ゼリー飲料の作製
３Ｌ容ステンレス製ジョッキにイオン交換水１５００ｇをとり、これにクエン酸（和光純薬工業社製）２．５５ｇとクエン酸ナトリウム（和光純薬工業社製）１．８０ｇを加え、攪拌機（製品名：スリーワンモーター、形式：ＦＢＬ−６００；ＨＥＩＤＯＮ社製）にて混合して溶解した。そこに粉寒天（商品名：伊那寒天Ｓ−７；伊那食品工業社製）１２．０ｇ、カラギーナン（商品名：ＧＥＮＵＧＥＬカラギナン ＷＧ−１０８；ＣＰ Ｋｅｌｃｏ社製）７．５ｇを加え、攪拌機（製品名：スリーワンモーター 形式：ＦＢＬ−６００；ＨＥＩＤＯＮ社製）１５０ｒｐｍで、６０℃・５分間混合してゼリー飲料を得た。得られたゼリー飲料（品温２５℃）をｐＨメータ（型式：ＨＭ−３０Ｒ；東亜ディ−ケーケー社製）で測定したところｐＨ３．８であった。[Food containing a powdery fat-soluble vitamin-containing composition]
(1) Preparation of jelly beverage Take 1500 g of ion-exchanged water in a 3 L stainless steel mug, add 2.55 g of citric acid (Wako Pure Chemical Industries) and 1.80 g of sodium citrate (Wako Pure Chemical Industries) Were mixed and dissolved by a stirrer (product name: Three-One Motor, Model: FBL-600; manufactured by HEIDON). 12.0 g of powdered agar (trade name: Ina Agar S-7; manufactured by Ina Food Industry Co., Ltd.) and 7.5 g of carrageenan (trade name: GENUGEL carrageenan WG-108; manufactured by CP Kelco) were added, and a stirrer (product name). : Three-one motor Format: FBL-600; manufactured by HEIDON) The mixture was mixed at 150 rpm at 60 ° C. for 5 minutes to obtain a jelly beverage. The obtained jelly beverage (product temperature 25 ° C.) was measured with a pH meter (model: HM-30R; manufactured by Toa DKK Corporation), and it was pH 3.8.
（２）原材料
ゼリー飲料（（１）ゼリー飲料の作製で得たゼリー飲料 ｐＨ３．８）
実施例品７（ビタミンＡと炭酸カリウムを含有する粉末状の脂溶性ビタミン含有組成物）
実施例品８（ビタミンＡと炭酸カリウムを含有する粉末状の脂溶性ビタミン含有組成物）
比較例品６（ビタミンＡを含有する粉末状の脂溶性ビタミン含有組成物）
比較例品７（ビタミンＡを含有する粉末状の脂溶性ビタミン含有組成物）(2) Raw material jelly drink ((1) Jelly drink obtained by preparation of jelly drink pH 3.8)
Example product 7 (powdered fat-soluble vitamin-containing composition containing vitamin A and potassium carbonate)
Example product 8 (powdered fat-soluble vitamin-containing composition containing vitamin A and potassium carbonate)
Comparative product 6 (powdered fat-soluble vitamin-containing composition containing vitamin A)
Comparative product 7 (powdered fat-soluble vitamin-containing composition containing vitamin A)
（３）粉末状の脂溶性ビタミン含有組成物を含有する食品の配合
上記原材料を用いて作製した粉末の脂溶性ビタミン含有組成物を含有する食品の配合を表５に示した。(3) Formulation of food containing powdery fat-soluble vitamin-containing composition Table 5 shows the formulation of food containing the powdered fat-soluble vitamin-containing composition prepared using the above raw materials.
表中の数値は、質量（ｇ）である。 The numerical value in a table | surface is mass (g).
（４）粉末状の脂溶性ビタミン含有組成物を含有する食品の作製方法
表５のゼリー飲料３００ｇを５００ｍL容ビーカーにとり、攪拌機（製品名：スリーワンモーター 形式：ＦＢＬ−６００；ＨＥＩＤＯＮ社製）にて攪拌しながら加温して８５℃に保温して、攪拌しながら粉末状の脂溶性ビタミン含有組成物（実施例品７、８ 比較例品６、７）各０．０９ｇを加えて撹拌し、粉末状の脂溶性ビタミン含有組成物を含むゼリー飲料（実施例品９、１０ 比較例品８、９）を得た。(4) Preparation method of food containing powdery fat-soluble vitamin-containing composition Take 300 g of jelly beverage of Table 5 in a 500 mL beaker and stirrer (product name: Three-One Motor Model: FBL-600; manufactured by HEIDON) Heat with stirring and keep at 85 ° C. While stirring, add 0.09 g of each powdered fat-soluble vitamin-containing composition (Example Product 7, 8 Comparative Product 6, 7), and stir. The jelly drink (Example product 9, 10 comparative example products 8 and 9) containing a powdery fat-soluble vitamin containing composition was obtained.
（５）粉末状の脂溶性ビタミン含有組成物を含むゼリー飲料の安定性試験方法及び結果
得られた粉末状の脂溶性ビタミン含有組成物を含むゼリー飲料１００ｇを、レトルトパウチに加えて密封した後、高圧蒸気滅菌装置（オートクレーブ 型式：ＳＮ２００；ヤマト科学社製）にて１０５℃、２分間の加熱殺菌処理を行った。次いで殺菌済みの粉末状の脂溶性ビタミン含有組成物を含むゼリー飲料を、４０℃暗所で１４日間、２８日間保管した。
粉末状の脂溶性ビタミン含有組成物を含有するゼリー飲料中に含まれる脂溶性ビタミン（ビタミンＡ）含量を測定し、作製直後（加熱殺菌前）の脂溶性ビタミン含量を１００質量％として殺菌後、保管後の脂溶性ビタミンの含有質量％（残存率）を計算した。結果を表６に示す。(5) Stability test method and result of jelly beverage containing powdery fat-soluble vitamin-containing composition After adding 100 g of jelly beverage containing the obtained powdery fat-soluble vitamin-containing composition to the retort pouch and sealing it Then, heat sterilization treatment was performed at 105 ° C. for 2 minutes in a high-pressure steam sterilizer (autoclave model: SN200; manufactured by Yamato Kagaku Co., Ltd.). Subsequently, the jelly drink containing the sterilized powdery fat-soluble vitamin-containing composition was stored in a dark place at 40 ° C. for 14 days and 28 days.
After measuring the fat-soluble vitamin (vitamin A) content contained in the jelly beverage containing the powdery fat-soluble vitamin-containing composition, and sterilizing the fat-soluble vitamin content immediately after production (before heat sterilization) as 100% by mass, The mass% (residual rate) of the fat-soluble vitamin after storage was calculated. The results are shown in Table 6.
表中の数値は、作成直後（加熱殺菌前）の脂溶性ビタミンを１００質量％として保管後の脂溶性ビタミンの各含有質量％（残存率）である。 The numerical values in the table are each mass% (residual rate) of the fat-soluble vitamin after storage, with the fat-soluble vitamin immediately after preparation (before heat sterilization) being 100 mass%.
表中に示した結果より判明するように、炭酸カリウムを含有する粉末状の脂溶性ビタミン含有組成物は、ゼリー飲料中においても脂溶性ビタミンの残存率が高く保管時の安定性は良好な結果であった。一方、炭酸カリウムを含有しない脂溶性ビタミン含有組成物は、ゼリー飲料中での保管時の安定性は低く悪い結果であった。 As can be seen from the results shown in the table, the powdered fat-soluble vitamin-containing composition containing potassium carbonate has a high residual ratio of fat-soluble vitamins even in jelly beverages and good stability during storage. Met. On the other hand, the fat-soluble vitamin-containing composition containing no potassium carbonate has a poor stability when stored in a jelly beverage.
本発明により、安定性が向上した脂溶性ビタミン含有組成物が提供される。本発明が提供する脂溶性ビタミン含有組成物は安定であり、脂溶性ビタミンの含量低下が抑制され品質上の経時変化が少なく、さらに脂溶性ビタミン含有組成物を飲食品に配合しても脂溶性ビタミンの含量低下が抑制される点で、産業上の利用性は多大なものである。 According to the present invention, a fat-soluble vitamin-containing composition having improved stability is provided. The fat-soluble vitamin-containing composition provided by the present invention is stable, suppresses a decrease in the content of the fat-soluble vitamin, has little change in quality over time, and is fat-soluble even when the fat-soluble vitamin-containing composition is added to food and drink. Industrial applicability is tremendous in that the decrease in vitamin content is suppressed.
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Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5888323A (en) * | 1981-11-20 | 1983-05-26 | Riyoushiyoku Kenkyukai | Nutrient composition for infants |
| JPS59156254A (en) * | 1982-12-29 | 1984-09-05 | タバグ・アーゲー | Food having dental caries preventing property, toothpaste, drug, chewing gum and production thereof |
| JPS63192712A (en) * | 1987-02-06 | 1988-08-10 | Nippon Zeora Kk | Toothpaste |
| JPH02295463A (en) * | 1989-05-10 | 1990-12-06 | Eisai Co Ltd | Food material for improving hepatic function |
| JPH04197155A (en) * | 1990-11-28 | 1992-07-16 | Snow Brand Milk Prod Co Ltd | Iron-enriched liquid nourishing composition and its production |
| JPH04262762A (en) * | 1991-02-18 | 1992-09-18 | Terumo Corp | Food composition, food for preventing hyperphagia and production of food composition |
| JPH08143456A (en) * | 1994-11-21 | 1996-06-04 | Ikeda Shokken Kk | Powder containing fat-soluble vitamins and its production |
| JPH10101524A (en) * | 1996-09-29 | 1998-04-21 | Shiseido Co Ltd | Emulsified composition containing fat-soluble vitamin |
| JPH11152491A (en) * | 1997-11-21 | 1999-06-08 | Ueda Oils And Fats Mfg Co Ltd | Improvement of oxidation resistance of tocopherol |
| JP2003503324A (en) * | 1999-06-29 | 2003-01-28 | イクセル・ハーバシューティカルズ | Effervescent green tea extract preparation |
| JP2003292414A (en) * | 2002-04-01 | 2003-10-15 | Pias Arise Kk | Liposoluble component-stabilized composition and cosmetic formulated with the composition and method for stabilizing liposoluble component |
| JP2004285007A (en) * | 2003-03-24 | 2004-10-14 | En Otsuka Pharmaceutical Co Ltd | Deodorizing component-containing nutritive composition |
| JP2004283117A (en) * | 2003-03-24 | 2004-10-14 | En Otsuka Pharmaceutical Co Ltd | Liquid nutrition composed kit having lactoprotein as main nitrogen source |
-
2010
- 2010-08-20 WO PCT/JP2010/064065 patent/WO2011040141A1/en active Application Filing
- 2010-08-20 JP JP2011534136A patent/JP5836127B2/en active Active
- 2010-08-20 CN CN2010800434585A patent/CN102639011A/en active Pending
- 2010-09-29 TW TW099132926A patent/TWI531321B/en not_active IP Right Cessation
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5888323A (en) * | 1981-11-20 | 1983-05-26 | Riyoushiyoku Kenkyukai | Nutrient composition for infants |
| JPS59156254A (en) * | 1982-12-29 | 1984-09-05 | タバグ・アーゲー | Food having dental caries preventing property, toothpaste, drug, chewing gum and production thereof |
| JPS63192712A (en) * | 1987-02-06 | 1988-08-10 | Nippon Zeora Kk | Toothpaste |
| JPH02295463A (en) * | 1989-05-10 | 1990-12-06 | Eisai Co Ltd | Food material for improving hepatic function |
| JPH04197155A (en) * | 1990-11-28 | 1992-07-16 | Snow Brand Milk Prod Co Ltd | Iron-enriched liquid nourishing composition and its production |
| JPH04262762A (en) * | 1991-02-18 | 1992-09-18 | Terumo Corp | Food composition, food for preventing hyperphagia and production of food composition |
| JPH08143456A (en) * | 1994-11-21 | 1996-06-04 | Ikeda Shokken Kk | Powder containing fat-soluble vitamins and its production |
| JPH10101524A (en) * | 1996-09-29 | 1998-04-21 | Shiseido Co Ltd | Emulsified composition containing fat-soluble vitamin |
| JPH11152491A (en) * | 1997-11-21 | 1999-06-08 | Ueda Oils And Fats Mfg Co Ltd | Improvement of oxidation resistance of tocopherol |
| JP2003503324A (en) * | 1999-06-29 | 2003-01-28 | イクセル・ハーバシューティカルズ | Effervescent green tea extract preparation |
| JP2003292414A (en) * | 2002-04-01 | 2003-10-15 | Pias Arise Kk | Liposoluble component-stabilized composition and cosmetic formulated with the composition and method for stabilizing liposoluble component |
| JP2004285007A (en) * | 2003-03-24 | 2004-10-14 | En Otsuka Pharmaceutical Co Ltd | Deodorizing component-containing nutritive composition |
| JP2004283117A (en) * | 2003-03-24 | 2004-10-14 | En Otsuka Pharmaceutical Co Ltd | Liquid nutrition composed kit having lactoprotein as main nitrogen source |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102639011A (en) | 2012-08-15 |
| TWI531321B (en) | 2016-05-01 |
| JPWO2011040141A1 (en) | 2013-02-21 |
| WO2011040141A1 (en) | 2011-04-07 |
| TW201117734A (en) | 2011-06-01 |
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