JP5828383B2 - Intraoral disinfectant and method for producing the same - Google Patents
Intraoral disinfectant and method for producing the same Download PDFInfo
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Description
本発明は、口腔内殺菌液及びその製造方法に関する。 The present invention relates to an oral bactericidal solution and a method for producing the same .
我が国は海域に囲まれており、水産資源も豊富なことから、古くから水産加工業が主要な産業として発展してきた。水揚げされたカキやホタテ等では、食用とされている部位以外の貝殻は加工残渣となり、漁業系廃棄物（未利用資源）として排出され、土木資材、稚貝の養殖資材、土壌改良材、飼料、肥料などに利用されている。 Since Japan is surrounded by sea areas and has abundant fishery resources, the fishery processing industry has long been developed as a major industry. In landed oysters and scallops, shells other than edible parts become processed residues and are discharged as fishery waste (unused resources). Civil engineering materials, juvenile culture materials, soil improvement materials, feed It is used for fertilizer.
しかし、まだ多くの貝殻が再利用されておらず、廃棄されているのが実情である。このため、これら未利用の貝殻の再利用技術が提案されている。 However, the situation is that many shells are not reused and are discarded. For this reason, a technique for reusing these unused shells has been proposed.
貝殻にはカルシウム分を豊富に含有しており、貝殻を原料に用いたカルシウムイオン水やセメント硬化体の製造など、貝殻の再利用が提案されている（例えば、特許文献１）。 Shells contain abundant calcium content, and reuse of shells has been proposed, such as the production of calcium ionized water and cement hardened bodies using shells as raw materials (for example, Patent Document 1).
特許文献１では、貝殻を焼成して得られる酸化カルシウムを酸性水溶液に溶解するカルシウムイオン水の製造方法、並びに、このカルシウムイオン水を用いたセメント硬化体の製造方法について開示されている。しかし、カルシウムイオン水の殺菌効果について何ら開示されていない。 Patent Document 1 discloses a method for producing calcium ion water in which calcium oxide obtained by baking shells is dissolved in an acidic aqueous solution, and a method for producing a hardened cement body using this calcium ion water. However, nothing is disclosed about the bactericidal effect of calcium ion water.
本発明は上記事項に鑑みてなされたものであり、その目的とするところは、殺菌効果を有し、殺菌効果の持続性が高い殺菌液を提供することにある。 This invention is made | formed in view of the said matter, The place made into the objective is to provide the bactericidal solution which has a bactericidal effect and the sustainability of a bactericidal effect is high.
本発明の第１の観点に係る口腔内殺菌液は、
カルシウム濃度が４ｇ／Ｌ〜３２ｇ／Ｌ、ｐＨが５．５〜９．５であるカルシウムイオン水を主成分として含有する、
ことを特徴とする。
The oral bactericidal solution according to the first aspect of the present invention is:
Containing calcium ion water having a calcium concentration of 4 g / L to 32 g / L and a pH of 5.5 to 9.5 as a main component,
It is characterized by that.
また、カルシウム濃度が６．４ｇ／Ｌ〜３２ｇ／Ｌであることが好ましい。 Further, the calcium concentration is preferably 6.4 g / L to 32 g / L.
本発明の第２の観点に係る口腔内殺菌液の製造方法は、
焼成した所定量の貝殻を酸性水溶液に溶解し、ｐＨ調整剤を添加して、カルシウム濃度が４ｇ／Ｌ〜３２ｇ／Ｌ、ｐＨが５．５〜９．５であるカルシウムイオン水を主成分として含有する口腔内殺菌液を得る、
ことを特徴とする。
The method for producing an oral bactericidal solution according to the second aspect of the present invention ,
A predetermined amount of shells baked form dissolved in an acidic aqueous solution, the main component by adding a pH adjusting agent, a calcium concentration of 4g / L~32g / L, the calcium ion water at a pH is 5.5 to 9.5 To obtain an oral bactericidal solution contained as
It is characterized by that .
また、前記酸性水溶液が酢酸水溶液であることが好ましい。 The acidic aqueous solution is preferably an acetic acid aqueous solution.
また、前記ｐＨ調整剤が炭酸水素ナトリウムであることが好ましい。 Moreover, it is preferable that the said pH adjuster is sodium hydrogencarbonate.
本発明に係る口腔内殺菌液は、殺菌効果に優れ、殺菌効果の持続性が高いという利点を有する。
The intraoral sterilizing solution according to the present invention has an advantage of being excellent in sterilizing effect and having high sterilizing effect .
本実施の形態に係る殺菌液は、カルシウム濃度が１．３ｇ／Ｌ〜３２ｇ／Ｌであり、ｐＨが５．５以上であるカルシウムイオン水を主成分として含有している。殺菌液は、原理は明らかではないが、殺菌効果を有するとともに、殺菌効果の持続性が高い。 The sterilizing liquid according to the present embodiment contains calcium ionized water having a calcium concentration of 1.3 g / L to 32 g / L and a pH of 5.5 or more as a main component. Although the principle is not clear, the sterilizing liquid has a sterilizing effect and has a high sterilizing effect.
殺菌液中では、後述の製造方法に記すように、酸性水溶液に酸化カルシウムを溶解して得られるため、カルシウムイオンが解離した状態を安定的に保つ。 In the sterilizing solution, as described in the production method described later, since it is obtained by dissolving calcium oxide in an acidic aqueous solution, the state in which calcium ions are dissociated is stably maintained.
殺菌液は、食品工場における床、壁、配管や食器類などの殺菌、或いは、医療現場における医療器具や医療機器等の殺菌など、殺菌が要求される種々の用途に用いることができる。殺菌液のｐＨ及びカルシウム濃度は、用いる用途に応じ、上記の範囲で適宜設定される。 The sterilizing liquid can be used for various applications that require sterilization, such as sterilization of floors, walls, piping, tableware and the like in food factories, or sterilization of medical instruments and medical equipment in medical sites. The pH and calcium concentration of the sterilizing solution are appropriately set within the above range according to the intended use.
例えば、殺菌液は、口腔内殺菌液として好適に用いることができる。口腔内殺菌液の場合、カルシウム濃度が３．２ｇ／Ｌ〜３２ｇ／Ｌであることが好ましい。カルシウム濃度が上記範囲であれば、口腔内の殺菌効果が高く、そして、殺菌効果の持続性が高い。より好ましくは、カルシウム濃度が４ｇ／Ｌ以上である。後述の実施例に示すように、含嗽２時間後の細菌の残存率（含嗽前の細菌数に対する含嗽後の細菌数の割合）が市販されている含嗽液に比べて低く、殺菌効果の持続性がより高い。更に好ましくは、カルシウム濃度が６．４ｇ／Ｌ以上である。なお、カルシウムの飽和濃度が３２ｇ／Ｌであるため、３２ｇ／Ｌを超えるとカルシウムがイオン化せず析出することになるので殺菌効果は望めない。 For example, the sterilizing liquid can be suitably used as an oral bactericidal liquid. In the case of an oral bactericidal solution, the calcium concentration is preferably 3.2 g / L to 32 g / L. When the calcium concentration is in the above range, the bactericidal effect in the oral cavity is high and the bactericidal effect is highly durable. More preferably, the calcium concentration is 4 g / L or more. As shown in the examples described later, the residual rate of bacteria after 2 hours of gargle (ratio of the number of bacteria after gargle to the number of bacteria before gargle) is lower than that of commercially available gargle liquids, and the persistence of the bactericidal effect Is higher. More preferably, the calcium concentration is 6.4 g / L or more. In addition, since the saturation density | concentration of calcium is 32 g / L, when it exceeds 32 g / L, since calcium will not ionize and will precipitate, a bactericidal effect cannot be expected.
また、口腔内殺菌液は、ｐＨは５．５〜９．５であることが好ましい。ｐＨが９．５より高いと人体に悪影響を及ぼしかねない。ｐＨが９．５以下であれば、アルカリイオン整水器協議会において、臨床試験にて安全性・有効性が科学的に確認されている範囲であり、人体への支障はないものと考えられる。また、ｐHが５．５より下回ると、殺菌効果の持続性が劣ることになる。 Further, the bactericidal solution in the oral cavity preferably has a pH of 5.5 to 9.5. If the pH is higher than 9.5, the human body may be adversely affected. If the pH is 9.5 or less, it is considered that there is no obstacle to the human body within the range where safety and effectiveness are scientifically confirmed in clinical trials at the Alkaline Water Concentrator Council. . Moreover, when pH is less than 5.5, the sustainability of the bactericidal effect is inferior.
口腔内殺菌液は、本発明の目的を損なわない範囲で、液体歯磨剤や洗口剤等に使用しうることが知られている各種の物質を含有していてもよい。例えば、精製水、エタノール等の溶剤、グリセリン、ソルビトール、プロピレングリコール、ポリエチレングリコール等の湿潤剤、ラウリル硫酸ナトリウム、ラウロイルサルコシンナトリウム等の発泡剤、パラベン、安息香酸、塩酸アルキルジアミノエチルグリシン等の防腐剤、サッカリンナトリウム、ステビアエキス等の甘味料、ハッカ油、スペアミント油、調合香料、メントール等の香料、その他フッ化物、銅クロロフィリンの金属塩、酢酸トコフェロール、グリチルリチン酸及びその塩類、塩化ナトリウム、アラントイン誘導体、イソプロピルメチルフェノール、エピジヒドロコレステリン、グアイアズレン及びその塩類、クロルヘキシジン類、エデト酸塩、炭酸水素ナトリウムなどの抗炎症剤、殺菌剤、酵素等の薬効成分などを適宜含有していてもよい。 The oral bactericidal solution may contain various substances that are known to be usable for liquid dentifrices, mouthwashes, and the like as long as the object of the present invention is not impaired. For example, purified water, solvents such as ethanol, wetting agents such as glycerin, sorbitol, propylene glycol, polyethylene glycol, foaming agents such as sodium lauryl sulfate, sodium lauroyl sarcosine, and preservatives such as parabens, benzoic acid, alkyldiaminoethylglycine hydrochloride , Sweeteners such as sodium saccharin, stevia extract, mint oil, spearmint oil, blended fragrances, fragrances such as menthol, other fluorides, metal salts of copper chlorophyllin, tocopherol acetate, glycyrrhizic acid and its salts, sodium chloride, allantoin derivatives, isopropyl Methylphenol, epidihydrocholesterin, guaiazulene and its salts, chlorhexidine, edetate, sodium bicarbonate and other anti-inflammatory agents, fungicides, and medicinal components such as enzymes It may have.
殺菌液は、一例として、以下のように貝殻を原料として得られる。貝殻の主成分は炭酸カルシウム（ＣａＣＯ３）であり、カルシウム分が豊富に含まれている。 As an example, the sterilizing liquid is obtained using shells as raw materials as follows. The main component of the shell is calcium carbonate (CaCO 3 ), which is rich in calcium.
まず、貝殻を焼成する。１０００〜１２００℃の温度範囲で貝殻を焼成することにより、貝殻の主成分である炭酸カルシウムが酸化カルシウム（ＣａＯ）になる。 First, the shell is fired. By baking the shell in a temperature range of 1000 to 1200 ° C., calcium carbonate, which is the main component of the shell, becomes calcium oxide (CaO).
この酸化カルシウムと炭酸水素ナトリウムとを酸性水溶液に添加し、貝殻粉末を溶解する。炭酸水素ナトリウムが分解して発生する炭酸ガスにより、酸化カルシウムの溶解が促進され、カルシウムイオンになる。なお、炭酸ガスによって炭酸カルシウムが生成するが、更に炭酸水素ナトリウムを添加し、過剰に炭酸ガスを発生させることで、生成した炭酸カルシウムが分解し、カルシウムイオンになる。 The calcium oxide and sodium bicarbonate are added to the acidic aqueous solution to dissolve the shell powder. Dissolution of calcium oxide is promoted by the carbon dioxide gas generated by decomposition of sodium hydrogen carbonate to become calcium ions. Calcium carbonate is produced by carbon dioxide, but by adding sodium hydrogencarbonate and generating carbon dioxide excessively, the produced calcium carbonate is decomposed and becomes calcium ions.
酸性水溶液として、酢酸水溶液、クエン酸水溶液、ギ酸水溶液、酒石酸水溶液等の水溶液を好適に用いることができる。なかでも酢酸水溶液を用いることが好ましい。酸化カルシウムの溶解が最も促進されるとともに、カルシウムの溶解度が高く、溶液中のカルシウムイオン濃度を高くすることができる。 As the acidic aqueous solution, an aqueous solution such as an acetic acid aqueous solution, a citric acid aqueous solution, a formic acid aqueous solution, or a tartaric acid aqueous solution can be suitably used. Of these, an acetic acid aqueous solution is preferably used. The dissolution of calcium oxide is most promoted, the solubility of calcium is high, and the calcium ion concentration in the solution can be increased.
なお、貝殻は、一般的に種々の重金属（カドミウム、鉛、ヒ素、水銀等）を含んでいるので、これらの重金属を除去することが好ましい。これらの重金属は溶解過程で重金属イオンとなり、酸化物や水酸化物、或いは炭酸塩を形成し、沈降する。このため、得られた水溶液を静置してこれらの酸化物等を沈降させ、上澄み液と分離し、分離した上澄み液を濾過することにより、不純物を除去したカルシウムイオン水を得ることができる。 In addition, since the shell generally contains various heavy metals (cadmium, lead, arsenic, mercury, etc.), it is preferable to remove these heavy metals. These heavy metals become heavy metal ions during the dissolution process, form oxides, hydroxides, or carbonates, and precipitate. For this reason, the obtained aqueous solution is allowed to stand to precipitate these oxides and the like, and is separated from the supernatant, and the separated supernatant is filtered to obtain calcium ion water from which impurities are removed.
そして、得られたカルシウムイオン水のｐＨが上述した範囲になるよう、ｐＨを調製する。ｐＨの調整は、カルシウムイオン水に炭酸水素ナトリウム等のｐＨ調整剤を添加して行えばよい。このようにして、殺菌液を得ることができる。 Then, the pH is adjusted so that the pH of the obtained calcium ion water is in the above-described range. The pH may be adjusted by adding a pH adjusting agent such as sodium hydrogen carbonate to calcium ion water. In this way, a sterilizing solution can be obtained.
また、カルシウム濃度の調整は、酸性水溶液に溶解させる焼成貝殻の量を適宜調節して上記範囲のカルシウム濃度に調節してもよい。 In addition, the calcium concentration may be adjusted to a calcium concentration within the above range by appropriately adjusting the amount of the baked shells dissolved in the acidic aqueous solution.
また、酸性水溶液に焼成貝殻を飽和状態まで溶解させた後に、水等の希釈液を加えて上記範囲のカルシウム濃度にしてもよい。 Moreover, after dissolving a baked shell in an acidic aqueous solution to a saturated state, a diluted solution such as water may be added to obtain a calcium concentration in the above range.
また、カルシウム濃度を高濃度に調製した殺菌液を用意しておき、使用する際に、上記のカルシウム濃度の範囲になるように、水等の希釈液で希釈して使用する形態であってもよい。 In addition, a sterilizing solution prepared with a high calcium concentration may be prepared, and when used, the sterilizing solution may be diluted with a diluent such as water so as to be in the above calcium concentration range. Good.
種々のカルシウム濃度及びｐＨの殺菌液を調整して用い、口腔内の殺菌効果を検証した。 Various bactericidal and pH bactericidal solutions were prepared and used to verify the bactericidal effect in the oral cavity.
上述の製造方法に従い、焼成したカキ殻を酢酸水溶液に溶解し、カルシウム濃度３２ｇ／Ｌのカルシウムイオン水を調製した。このカルシウムイオン水に４％生理食塩水を添加して、種々のカルシウム濃度に調整するとともに、炭酸水素カルシウム（ＮａＨＣＯ３）を添加して、種々のｐＨに調整し、殺菌液として用いた。 According to the above production method, the calcined oyster shell was dissolved in an acetic acid aqueous solution to prepare calcium ion water having a calcium concentration of 32 g / L. 4% physiological saline was added to this calcium ion water to adjust to various calcium concentrations, and calcium bicarbonate (NaHCO 3 ) was added to adjust to various pHs, which were used as a bactericidal solution.
用意した殺菌液のカルシウム濃度及びｐＨの組み合わせを表１に示す。 Table 1 shows combinations of calcium concentration and pH of the prepared sterilizing solution.
殺菌効果は以下のようにして評価した。 The bactericidal effect was evaluated as follows.
被験者は２０ｍＬの殺菌液を口に含み、１５秒間の含嗽を行った。この含嗽は、計３回行った。 The test subject contained 20 mL of bactericidal solution in his mouth and rubbed for 15 seconds. This gargle was performed three times in total.
殺菌液による含嗽１時間前、含嗽直後、含嗽６０分後及び含嗽１２０分後に、それぞれ生理食塩水１０ｍＬを使用し、１０秒間含嗽した。そして、これらの口腔洗浄液を生理食塩水で１０７倍まで希釈した各希釈液を５００μＬずつ血液寒天培地上に添加し、３５℃４８時間炭酸ガス培養した。培養後、各培地上のコロニーをコロニーカウンターで計測し、ＣＦＵ／ｍＬとして表示した。 The sterilization solution was used for 1 second before gargle, immediately after gargle, after 60 minutes of gargle and after 120 minutes of gargle, using 10 mL of physiological saline for 10 seconds. Then, each dilution was diluted to 10 7 times these mouth wash with saline was added onto blood agar every 500 [mu] L, and 35 ° C. 48 h carbon dioxide gas incubator. After culture, colonies on each medium were counted with a colony counter and displayed as CFU / mL.
そして、含嗽１時間前の細菌数を１００％とし、含嗽直後、含嗽６０分後、含嗽１２０分後の細菌数をカウントし、含嗽１時間前の細菌数に対するそれぞれの細菌数の割合（以下、この割合を細菌残存率（％）と記す）を算出して評価を行った。 Then, the number of bacteria one hour before gargle is taken as 100%, the number of bacteria immediately after gargle, 60 minutes after gargle, and 120 minutes after gargle is counted, and the ratio of the number of bacteria to the number of bacteria one hour before gargle (hereinafter, This ratio was described as bacterial residual rate (%)) and evaluated.
また、酢酸水溶液の代わりに乳酸水溶液を用いて調製した殺菌液（カルシウム濃度：６．４ｇ／Ｌ、ｐＨ：１２．１）（以下、殺菌液Ｄ１）を用い、上記と同様に行った。 Moreover, it carried out similarly to the above using the bactericidal solution (calcium concentration: 6.4 g / L, pH: 12.1) (henceforth, bactericidal solution D1) prepared using the lactic acid aqueous solution instead of the acetic acid aqueous solution.
更には、参照実験として、食塩水、及び、市販含嗽液（商品名：システマ薬用デンタルリンス、ライオン株式会社製）についても上記と同様に行った。 Further, as a reference experiment, saline solution and a commercially available gargle solution (trade name: systemic medicinal dental rinse, manufactured by Lion Corporation) were also used in the same manner as described above.
その結果を表２に示す。また、図１に殺菌液Ｂ２及び市販含嗽液の細菌残存率の経時変化を示す。また、図２に殺菌液のｐＨと細菌残存率の関係、図３に殺菌液のカルシウム濃度と細菌残存率の関係をそれぞれ示す。 The results are shown in Table 2. In addition, FIG. 1 shows the change over time of the bacteria remaining rate of the bactericidal solution B2 and the commercial gargle. FIG. 2 shows the relationship between the pH of the sterilizing solution and the bacterial survival rate, and FIG. 3 shows the relationship between the calcium concentration of the sterilizing solution and the bacterial survival rate.
食塩水による含嗽効果は、含嗽１時間後には、ほぼ消失していた。 The gargle effect by the saline solution almost disappeared after 1 hour of gargle.
また、市販含嗽液では、含嗽直後に細菌数が含嗽１時間前に比べて４０％に減少した。しかし、含嗽１時間後では含嗽直後の細菌数から増加に転じており、含嗽２時間後では更に増加していた。即ち、含嗽直後では殺菌効果が高いものの、殺菌効果が続かず、持続性が低いことがわかった。 In addition, in the commercially available garnished liquid, the number of bacteria decreased to 40% immediately after gargle compared to 1 hour before gargle. However, after 1 hour of rinsing, the number of bacteria immediately after rinsing started to increase, and after 2 hours of rinsing, it increased further. That is, it was found that the bactericidal effect was high immediately after gargling, but the bactericidal effect was not continued and the sustainability was low.
一方、殺菌液では、含嗽直後に市販含嗽液ほどの細菌数の大幅な減少は見られなかった。しかしながら、含嗽１時間後、２時間後にも殺菌効果が持続する傾向が見られる。 On the other hand, in the bactericidal solution, the number of bacteria was not significantly reduced immediately after the rinsing as in the case of the commercially available gargle. However, the sterilizing effect tends to be maintained even after 1 hour of gargle and after 2 hours.
特に、図１に示しているように、殺菌液Ｂ２（ｐＨ：８．１、カルシウム濃度：６．４ｇ／Ｌ）では、含嗽直後、含嗽１時間後、含嗽２時間後と順に細菌数が減少しており、含嗽２時間後では、市販含嗽液よりも細菌数の残存率が低く良好である。したがって、殺菌効果の持続性が高いことが示された。 In particular, as shown in FIG. 1, in the bactericidal solution B2 (pH: 8.1, calcium concentration: 6.4 g / L), the number of bacteria decreases in the order of immediately after gargle, after 1 hour of gargle, and after 2 hours of gargle. After 2 hours of rinsing, the remaining number of bacteria is lower and better than the commercially available gargle. Therefore, it was shown that the persistence of the bactericidal effect is high.
また、図２は、カルシウム濃度６．４ｇ／Ｌの殺菌液Ａ２、Ｂ２、Ｃ３の含嗽２時間後における細菌残存率を、横軸をｐＨとして表したものである。図２を見ると、およそｐＨが５．５以上で、市販含嗽液の細菌残存率よりも良好である。したがって、ｐＨが５．５以上で長時間に渡り殺菌効果が持続するものと考えられる。 FIG. 2 shows the bacterial residual rate after 2 hours of sterilization with the sterilizing liquids A2, B2, and C3 having a calcium concentration of 6.4 g / L, with the horizontal axis representing pH. When FIG. 2 is seen, pH is about 5.5 or more, and it is better than the bacteria residual rate of a commercially available gargle. Therefore, it is considered that the bactericidal effect lasts for a long time at a pH of 5.5 or higher.
図３に、カルシウム濃度３．２ｇ／Ｌ、６．４ｇ／Ｌ、３２．０ｇ／Ｌの殺菌液Ｂ１、Ｂ２、Ｂ３の含嗽２時間後の細菌残存率を示している。図３を見ると、カルシウム濃度がおよそ４ｇ／Ｌ以上で、細菌残存率が市販含嗽液（６９％）よりも低くなっている。したがって、カルシウム濃度が４ｇ／Ｌ以上だと市販含嗽液に比べて殺菌効果が長時間持続する。なお、カルシウム濃度が６．４ｇ／Ｌ以上３２ｇ／Ｌ以下、特に１０ｇ／Ｌ以上３２ｇ／Ｌ以下では、殺菌効果の持続性はさほど変わらないことから、カルシウムのコスト等を考慮して適宜設定すればよいと考えられる。 FIG. 3 shows the bacteria remaining rate after 2 hours of sterilization with the bactericidal solutions B1, B2, and B3 having a calcium concentration of 3.2 g / L, 6.4 g / L, and 32.0 g / L. Referring to FIG. 3, the calcium concentration is about 4 g / L or more, and the bacterial residual rate is lower than the commercially available gargle (69%). Therefore, when the calcium concentration is 4 g / L or more, the bactericidal effect lasts for a long time compared to the commercially available gargle. It should be noted that when the calcium concentration is 6.4 g / L or more and 32 g / L or less, particularly 10 g / L or more and 32 g / L or less, the persistence of the bactericidal effect does not change so much. I think it would be good.
なお、殺菌液Ｄ１では、殺菌効果が見受けられなかった。殺菌液Ｄ１は乳酸水溶液に酸化カルシウムを溶解して調整しており、カルシウムの溶解度が酢酸水溶液に比べて低く、カルシウムイオンの解離状態が安定していなかったためと考えられる。 In addition, the bactericidal effect was not seen in the bactericidal solution D1. The sterilizing solution D1 is prepared by dissolving calcium oxide in a lactic acid aqueous solution, and the solubility of calcium is lower than that of an acetic acid aqueous solution, and the dissociation state of calcium ions is not stable.
カルシウムイオン水の器具消毒効果について検証した。 It verified about the instrument disinfection effect of calcium ion water.
まず、一般口腔内細菌１．０×１０６個／ｍＬの菌液を作成した。この菌液２００ｍＬ中に未使用の歯科矯正用のピンカッターを１０分間浸漬し、菌を付着させた。 First, a bacterial solution of 1.0 × 10 6 general oral bacteria / mL was prepared. An unused orthodontic pin cutter was immersed in 200 mL of this bacterial solution for 10 minutes to allow the bacteria to adhere.
菌を付着させた後、ピンカッターを菌液中から取り出し、各殺菌液１００ｍＬに１５分間浸漬した。 After attaching the bacteria, the pin cutter was taken out of the bacterial solution and immersed in 100 mL of each sterilizing solution for 15 minutes.
殺菌液は、実施例１にて調製したカルシウム濃度が６．４ｇ／Ｌで、ｐＨがそれぞれ５、８．１、１２．１の殺菌液Ａ２、Ｂ２、Ｃ３を用いた。 As the sterilizing solution, the sterilizing solutions A2, B2, and C3 having a calcium concentration of 6.4 g / L prepared in Example 1 and pH values of 5, 8.1, and 12.1, respectively were used.
その後、それぞれのピンカッターを１００ｍＬの生理食塩水中に移し、超音波による機械的振動を加え、ピンカッターに残存している付着菌を回収した。 Thereafter, each pin cutter was transferred into 100 mL of physiological saline, and mechanical vibrations by ultrasonic waves were applied to collect adherent bacteria remaining on the pin cutter.
回収した液（１０μＬ）と普通寒天培地とを混釈し、付着菌を培養（３７℃、２４時間）した。培養した後、形成されたコロニーの数から、ピンカッターに残存する生菌数を算出した。 The recovered liquid (10 μL) was mixed with a normal agar medium, and the attached bacteria were cultured (37 ° C., 24 hours). After culturing, the number of viable bacteria remaining on the pin cutter was calculated from the number of colonies formed.
また、対照実験として、生理食塩水１００ｍＬ、及び、市販の歯科小器具用の消毒剤（商品名：ラスノンメディカル、日本歯科薬品株式会社製）についても上記同様にそれぞれ行った。 Further, as a control experiment, 100 mL of physiological saline and a commercially available disinfectant for dental appliances (trade name: Rasnon Medical, manufactured by Nippon Dental Pharmaceutical Co., Ltd.) were also performed in the same manner as described above.
培養後の写真を図４（Ａ）〜（Ｅ）に示す。図４（Ａ）が生理食塩水、図４（Ｂ）が殺菌液Ａ２、図４（Ｃ）が殺菌液Ｂ２、図４（Ｄ）が殺菌液Ｃ３、図４（Ｅ）が市販消毒液を用いた場合の写真である。また、それぞれの算出した生菌数を表３に示す。 The photograph after culture | cultivation is shown to FIG. 4 (A) shows physiological saline, FIG. 4 (B) shows the sterilizing solution A2, FIG. 4 (C) shows the sterilizing solution B2, FIG. 4 (D) shows the sterilizing solution C3, and FIG. It is a photograph when used. In addition, Table 3 shows the calculated viable cell count.
殺菌液Ａ２、Ｂ２、Ｃ３の結果を見ると、ｐＨが高くなるにつれて、細菌が減少している。そして、ｐＨが１２．１の殺菌液Ｃ３では、市販消毒液と同様に、細菌を検出できなかった。この結果から、器具等の消毒液としても利用できることが立証された。 Looking at the results of the sterilizing liquids A2, B2, and C3, bacteria are decreasing as the pH increases. And in the disinfecting liquid C3 having a pH of 12.1, bacteria could not be detected like the commercially available disinfecting liquid. From this result, it was proved that it could be used as a disinfectant for instruments.
上述したように、殺菌液は殺菌効果に優れ、殺菌効果の持続性が高い。口腔内の殺菌や医療器具の殺菌等、殺菌が要求される様々な用途に利用可能である。 As described above, the sterilizing liquid has an excellent sterilizing effect and has a high sterilizing effect. It can be used in various applications where sterilization is required, such as sterilization in the oral cavity and medical instruments.
Claims (5)
ことを特徴とする口腔内殺菌液。 Containing calcium ion water having a calcium concentration of 4 g / L to 32 g / L and a pH of 5.5 to 9.5 as a main component,
An oral bactericidal solution characterized by that.
ことを特徴とする請求項１に記載の口腔内殺菌液。 The calcium concentration is 6.4 g / L to 32 g / L,
The oral bactericidal solution according to claim 1 .
ことを特徴とする口腔内殺菌液の製造方法。 A predetermined amount of shells baked form dissolved in an acidic aqueous solution, the main component by adding a pH adjusting agent, a calcium concentration of 4g / L~32g / L, the calcium ion water at a pH is 5.5 to 9.5 To obtain an oral bactericidal solution contained as
A method for producing an oral bactericidal solution.
ことを特徴とする請求項３に記載の口腔内殺菌液の製造方法。 The acidic aqueous solution is an acetic acid aqueous solution;
The method for producing an oral bactericidal solution according to claim 3 .
ことを特徴とする請求項３又は４に記載の口腔内殺菌液の製造方法。 The pH adjuster is sodium bicarbonate;
The method for producing an oral bactericidal solution according to claim 3 or 4 , wherein:
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