JP5744529B2 - Melanin production inhibitor - Google Patents
Melanin production inhibitor Download PDFInfo
- Publication number
- JP5744529B2 JP5744529B2 JP2011004892A JP2011004892A JP5744529B2 JP 5744529 B2 JP5744529 B2 JP 5744529B2 JP 2011004892 A JP2011004892 A JP 2011004892A JP 2011004892 A JP2011004892 A JP 2011004892A JP 5744529 B2 JP5744529 B2 JP 5744529B2
- Authority
- JP
- Japan
- Prior art keywords
- whitening
- tart cherry
- fruit juice
- cosmetic
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 239000003410 keratolytic agent Substances 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- HOVAGTYPODGVJG-UHFFFAOYSA-N methyl beta-galactoside Natural products COC1OC(CO)C(O)C(O)C1O HOVAGTYPODGVJG-UHFFFAOYSA-N 0.000 description 1
- FAARLWTXUUQFSN-UHFFFAOYSA-N methylellagic acid Natural products O1C(=O)C2=CC(O)=C(O)C3=C2C2=C1C(OC)=C(O)C=C2C(=O)O3 FAARLWTXUUQFSN-UHFFFAOYSA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 239000001788 mono and diglycerides of fatty acids Substances 0.000 description 1
- JXTPJDDICSTXJX-UHFFFAOYSA-N n-Triacontane Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC JXTPJDDICSTXJX-UHFFFAOYSA-N 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- 229960005323 phenoxyethanol Drugs 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 229940035044 sorbitan monolaurate Drugs 0.000 description 1
- 239000001593 sorbitan monooleate Substances 0.000 description 1
- 229940035049 sorbitan monooleate Drugs 0.000 description 1
- 235000011069 sorbitan monooleate Nutrition 0.000 description 1
- 229940032094 squalane Drugs 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- OULAJFUGPPVRBK-UHFFFAOYSA-N tetratriacontyl alcohol Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCO OULAJFUGPPVRBK-UHFFFAOYSA-N 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 229940042585 tocopherol acetate Drugs 0.000 description 1
- GYDJEQRTZSCIOI-LJGSYFOKSA-N tranexamic acid Chemical compound NC[C@H]1CC[C@H](C(O)=O)CC1 GYDJEQRTZSCIOI-LJGSYFOKSA-N 0.000 description 1
- 229960000401 tranexamic acid Drugs 0.000 description 1
- VLPFTAMPNXLGLX-UHFFFAOYSA-N trioctanoin Chemical compound CCCCCCCC(=O)OCC(OC(=O)CCCCCCC)COC(=O)CCCCCCC VLPFTAMPNXLGLX-UHFFFAOYSA-N 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 229920003169 water-soluble polymer Polymers 0.000 description 1
Landscapes
- Cosmetics (AREA)
Description
本発明は、タルトチェリー果実搾汁液及び／又はタルトチェリー果実抽出物を含有する美白用皮膚外用剤又は美白用化粧料に関し、更に詳細には、美白剤及びメラニン生成抑制剤に関する。 The present invention relates to a whitening skin external preparation or a whitening cosmetic containing a tart cherry fruit juice and / or a tart cherry fruit extract, and more particularly to a whitening agent and a melanin production inhibitor.
従来、皮膚外用剤や化粧料等の有効成分として、種々の植物由来の成分が利用されている。タルトチェリー（Ｐｒｕｎｕｓ ｃｅｒａｓｕｓ）から得られる抽出物についても皮膚外用剤、化粧料、食品や医薬品等への利用が種々提案されている（特許文献１〜４）。
しかし、上記いずれの特許文献にも該抽出物の美白効果についての記載はない。
Conventionally, various plant-derived components have been used as active ingredients such as external preparations for skin and cosmetics. Various uses of an extract obtained from tart cherry (Prunus cerasus) for external preparations for skin, cosmetics, foods, pharmaceuticals and the like have been proposed (Patent Documents 1 to 4).
However, none of the above patent documents describes the whitening effect of the extract.
従来、美白効果のある植物抽出物が種々知られているが、十分な美白効果を得るという観点から、実際には、アルブチンやアスコルビン酸およびその誘導体、コウジ酸、エラグ酸などの美白剤が汎用されている。しかし、これらの汎用されている美白剤は美白効果に優れるものの、皮膚外用剤又は化粧料中への配合に制約が多く、これらの美白剤を含有する皮膚外用剤又は化粧料は、経時的に変色もしくは着色、分離、および結晶析出等の種々の問題が生じ易い。
本発明は、上記問題に鑑みなされたものであり、皮膚外用剤又は化粧料（医薬部外品や薬用化粧料を含む）に容易に配合でき、美白効果が高い美白剤、及びそれを含有した安定性、安全性が良好で、高い美白効果のある美白用皮膚外用剤又は美白用化粧料を提供することを課題とする。
Conventionally, various plant extracts having a whitening effect are known, but from the viewpoint of obtaining a sufficient whitening effect, whitening agents such as arbutin, ascorbic acid and derivatives thereof, kojic acid, and ellagic acid are generally used. Has been. However, although these widely used whitening agents are excellent in whitening effect, there are many restrictions on blending into a skin external preparation or cosmetic, and skin external preparations or cosmetics containing these whitening agents are Various problems such as discoloration or coloring, separation, and crystal precipitation are likely to occur.
The present invention has been made in view of the above problems, and can be easily blended into a skin external preparation or cosmetic (including quasi-drugs and medicinal cosmetics), and contains a whitening agent having a high whitening effect and the same. It is an object of the present invention to provide a whitening skin external preparation or a whitening cosmetic that has good stability and safety and has a high whitening effect.
本発明は、前記課題を解決するために鋭意検討した結果、タルトチェリー（Ｐｒｕｎｕｓ ｃｅｒａｓｕｓ）の果実搾汁液及び／又は果実抽出物に優れた美白効果を見出し、この知見に基づいて本発明を完成させるに至った。すなわち本発明は、タルトチェリー（Ｐｒｕｎｕｓ ｃｅｒａｓｕｓ）の果実搾汁液及び／又は果実抽出物を有効成分とする、美白剤及びメラニン生成抑制剤、並びにこれらを含有する美白用皮膚外用剤又は美白用化粧料に関する。 As a result of intensive studies to solve the above problems, the present invention finds an excellent whitening effect in a fruit juice and / or fruit extract of tart cherry (Prunus cerasus), and completes the present invention based on this finding. It came to. That is, the present invention relates to a whitening agent and a melanin production inhibitor containing, as active ingredients, a fruit juice and / or fruit extract of tart cherry (Prunus cerasus), and a whitening skin external preparation or a whitening cosmetic containing these. About.
本発明によれば、タルトチェリー（Ｐｒｕｎｕｓ ｃｅｒａｓｕｓ）の果実搾汁液及び／又は果実抽出物を有効成分として用いることにより、植物由来の安定性、安全性の良好なメラニン生成抑制剤、美白剤、並びにそれを有効成分として含有する美白用皮膚外用剤又は美白用化粧料を提供することができる。さらに、水系剤型や乳化剤型等、剤型を選ばずに幅広く皮膚外用剤又は化粧料に容易に配合でき、肌感触も良好で高い美白効果のある美白用皮膚外用剤又は美白用化粧料を提供することができる。 According to the present invention, by using a fruit juice and / or fruit extract of tart cherry (Prunus cerasus) as an active ingredient, plant-derived stability, safety-safe melanin production inhibitor, whitening agent, and A skin whitening external preparation or a whitening cosmetic containing the same as an active ingredient can be provided. Furthermore, a whitening skin external preparation or whitening cosmetic that can be easily blended into a wide range of skin external preparations or cosmetics, regardless of the dosage form, such as an aqueous type or an emulsifier type, has a good skin feel and a high whitening effect. Can be provided.
以下、本発明について詳細に説明する。なお、本明細書において「〜」とはその前後に記載される数値を下限値及び上限値として含む意味で使用される。
タルトチェリーはバラ科に属する植物で学名Ｐｒｕｎｕｓ ｃｅｒａｓｕｓであり、スミノミザクラ、酸果オウトウ、サワーチェリー（ｓｏｕｒ ｃｈｅｒｒｙ）と呼ばれる。タルトチェリーは米国を中心に栽培されており、酸味が強く、ジャム、ジュースや健康食品等に使用されている。本発明に用いられる果実搾汁液及び／又は果実抽出物はＰｒｕｎｕｓ ｃｅｒａｓｕｓの果実から得られるものであるが、その種類や産地はとくに限定されない。
Hereinafter, the present invention will be described in detail. In the present specification, “to” is used to mean that the numerical values described before and after it are included as a lower limit value and an upper limit value.
Tart cherry is a plant belonging to the family Rosaceae and has the scientific name Prunus cerasus, and is called Sumi cherry, sour cherry, sour cherry. Tart cherries are cultivated mainly in the United States, have a strong acidity, and are used for jams, juices and health foods. The fruit juice and / or fruit extract used in the present invention is obtained from the fruit of Prunus cerasus, but the type and production area are not particularly limited.
上記タルトチェリー果実搾汁液は、タルトチェリーの果実を破砕、摩砕等することにより得ることができるが、さらにパルプ分離処理、ろ過清澄処理、酵素処理、濃縮処理、微細化処理、脱臭脱色処理等を行ってもよく、その調製法は特に限定されない。必要であれば、破砕、摩砕等の前に果実から果皮や種子を除去することができ、および破砕、摩砕等後に果実搾汁液中に残留する果皮や種子等を裏ごし、ろ過などにより除去することもできる。 The tart cherry fruit juice can be obtained by crushing, grinding, etc. the fruit of the tart cherry, but further pulp separation treatment, filtration clarification treatment, enzyme treatment, concentration treatment, refinement treatment, deodorization and decolorization treatment, etc. The preparation method is not particularly limited. If necessary, the skin and seeds can be removed from the fruit before crushing, grinding, etc., and the skin and seeds remaining in the fruit juice after crushing, grinding, etc. are lined and removed by filtration, etc. You can also
また、本発明ではタルトチェリー果実の抽出物も使用することができる。タルトチェリー果実抽出物はタルトチェリー果実から種々の抽出溶媒を用いることによって得られる。その抽出方法に関しては特に限定されないが、適当な溶媒を用いて低温もしくは室温ないし加温下で抽出される。抽出溶媒としては、例えば水；メタノール、エタノール、プロパノール、イソプロパノール等の低級１価アルコール；グリセリン、プロピレングリコール、１，３−ブチレングリコール等の液状多価アルコール；アセトン等のケトン類；エチルエーテル、プロピルエーテル等のエーテル類；酢酸エチル等のエステル類が挙げられ、これらの１種または２種以上を選択して用いることができる。また、生理食塩水やリン酸緩衝液、リン酸緩衝生理食塩水等を用いても良い。 In the present invention, an extract of tart cherry fruit can also be used. Tart cherry fruit extract is obtained from tart cherry fruit by using various extraction solvents. The extraction method is not particularly limited, but the extraction is performed at a low temperature or at room temperature or under heating using a suitable solvent. Examples of the extraction solvent include water; lower monohydric alcohols such as methanol, ethanol, propanol and isopropanol; liquid polyhydric alcohols such as glycerin, propylene glycol and 1,3-butylene glycol; ketones such as acetone; ethyl ether and propyl Examples include ethers such as ether; esters such as ethyl acetate, and one or more of these can be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used.
抽出方法についても、その設定温度や時間等に特に制限はないが、例えば、含水濃度０〜１００ｖｏｌ％のエタノールまたは１，３−ブチレングリコールを用い、低温で、または室温で、ないしは加温して１〜５日間抽出を行ったのちろ過し、得られたろ液をさらに１週間ほど放置して熟成させ、再びろ過を行う方法が挙げられる。抽出中は系内を攪拌するのが好ましい。また、抽出操作の前にタルトチェリーに対して粉砕、細切、圧搾または発酵等の前処理を行うこともできる。 The extraction method is not particularly limited in the set temperature, time, etc. For example, ethanol or 1,3-butylene glycol having a water content of 0 to 100 vol% is used at low temperature, room temperature, or warmed. An example is a method in which extraction is performed for 1 to 5 days, followed by filtration, and the obtained filtrate is further left to mature for about one week, followed by filtration again. It is preferable to stir the system during extraction. In addition, pretreatment such as pulverization, chopping, pressing or fermentation can be performed on the tart cherry before the extraction operation.
得られた果実搾汁液及び／又は果実抽出物は溶液状の他に必要に応じてペースト状、ゲル状、固体状、粉末状とすることもできる。果実搾汁液及び／又は果実抽出物は調製後そのまま使用することができるが、濃縮または乾固またはスプレードライまたは凍結乾燥したものを水や極性溶媒に再度溶解して用いても良い。 The obtained fruit juice and / or fruit extract can be in the form of a paste, gel, solid, or powder as needed in addition to the solution. Although the fruit juice and / or fruit extract can be used as it is after preparation, it may be used after being concentrated, dried, spray-dried or freeze-dried and dissolved again in water or a polar solvent.
溶媒としては、例えば水；メタノール、エタノール等の低級１価アルコール；グリセリン、プロピレングリコール、１，３−ブチレングリコール等の液状多価アルコール等の１種または２種以上を用いることができる。または防腐対応のため、パラオキシ安息香酸エステル、フェノキシエタノール等の防腐剤の１種または２種以上を加えて用いることもできる。 As the solvent, for example, one or more of water; lower monohydric alcohols such as methanol and ethanol; and liquid polyhydric alcohols such as glycerin, propylene glycol, and 1,3-butylene glycol can be used. Alternatively, for preserving, one or more preservatives such as paraoxybenzoic acid ester and phenoxyethanol may be added and used.
さらに、必要であれば本発明の効果に影響のない範囲でろ過またはイオン交換樹脂等により脱色、脱臭、脱塩などの処理を施して用いることもできる。また、限外ろ過やイオン交換クロマトグラフィー、ゲルろ過クロマトグラフィーなどの方法により分画して用いることもできる。 Furthermore, if necessary, it can be used after being subjected to treatment such as decolorization, deodorization, and desalting with filtration or ion exchange resin within a range that does not affect the effect of the present invention. Moreover, it can also fractionate and use by methods, such as ultrafiltration, ion-exchange chromatography, and gel filtration chromatography.
このようにして得られたタルトチェリー（Ｐｒｕｎｕｓ ｃｅｒａｓｕｓ）の果実搾汁液及び／又は果実抽出物を有効成分とする美白剤は、メラニン生成を抑制する等し、皮膚のシミ、ソバカスを防ぐ優れた美白効果を発揮する。 The whitening agent containing the fruit juice and / or fruit extract of tart cherry (Prunus cerasus) thus obtained as an active ingredient suppresses melanin production and the like, and prevents white spots and freckles on the skin. Demonstrate the effect.
本発明の美白用皮膚外用剤又は美白用化粧料におけるタルトチェリー果実搾汁液及び／又は果実抽出物の含有量は、乾燥固形分で０．０００１〜０．１質量％（以下、単に「％」と記載する）であるのが好ましく、０．０００１〜０．０１％であるのがより好ましい。前記範囲であると、高い美白効果が得られるとともに、皮膚外用剤又は化粧料中に安定的に配合することができる。 The content of the tart cherry fruit juice and / or the fruit extract in the skin whitening external preparation or whitening cosmetic of the present invention is 0.0001 to 0.1% by mass (hereinafter simply referred to as “%”) in terms of dry solid content. It is preferable to be 0.0001 to 0.01%. When it is in the above range, a high whitening effect can be obtained, and it can be stably blended in a skin external preparation or cosmetic.
本発明の美白用皮膚外用剤又は美白用化粧料には、本発明の美白剤とともに、他の美白剤を含有してもよい。他の美白剤の例には、アスコルビン酸又はその誘導体、アルブチン、リノール酸、トラネキサム酸およびその誘導体、カミツレ抽出物等が含まれる。他の美白剤としてはアスコルビン酸又はその誘導体およびアルブチンから選ばれる一種以上が好ましい。これらの美白剤は高い美白効果を奏するが、一方高濃度配合が経時安定性および使用感の点で困難である。本発明の美白剤と併用することで、経時安定性および使用感を損なうことなく、従来の美白用皮膚外用剤又は美白用化粧料以上の美白効果を得ることができる。 The whitening skin external preparation or whitening cosmetic of the present invention may contain other whitening agents in addition to the whitening agent of the present invention. Examples of other whitening agents include ascorbic acid or derivatives thereof, arbutin, linoleic acid, tranexamic acid and derivatives thereof, chamomile extract and the like. Other whitening agents are preferably one or more selected from ascorbic acid or a derivative thereof and arbutin. Although these whitening agents have a high whitening effect, high concentration blending is difficult in terms of stability over time and usability. By using together with the whitening agent of the present invention, it is possible to obtain a whitening effect more than that of a conventional whitening skin external preparation or whitening cosmetic without impairing the stability over time and the feeling of use.
また、本発明の美白用皮膚外用剤又は美白用化粧料を調製するにあたり、皮膚外用剤、化粧料等の製造に通常使用される成分、即ち、水（精製水、温泉水、深層水等）、アルコール類、油剤、界面活性剤、増粘剤、金属セッケン、ゲル化剤、粉体、キレート剤、水溶性高分子、皮膜形成剤、樹脂、包接化合物、抗菌剤、消臭剤、塩類、ｐＨ調整剤、紫外線吸収剤、動植物・微生物由来の抽出物、角質溶解剤、酵素、ホルモン類、他のビタミン類、保湿剤・活性酸素除去剤・血行促進剤・収斂剤・抗脂漏剤・抗炎症剤・細胞賦活剤・清涼剤等の各種薬効剤、香料等を、本発明の効果を損なわない範囲で適宜加えることができるが、これらになんら限定されることはない。 In preparing the skin whitening preparation or skin whitening cosmetic of the present invention, the components usually used in the manufacture of skin external preparations, cosmetics and the like, that is, water (purified water, hot spring water, deep water, etc.) , Alcohols, oils, surfactants, thickeners, metal soaps, gelling agents, powders, chelating agents, water-soluble polymers, film forming agents, resins, inclusion compounds, antibacterial agents, deodorants, salts , PH adjusters, UV absorbers, extracts from animals, plants and microorganisms, keratolytic agents, enzymes, hormones, other vitamins, moisturizers, active oxygen removers, blood circulation promoters, astringents, antiseborrheic agents -Various medicinal agents such as anti-inflammatory agents, cell activators, and refreshing agents, fragrances, and the like can be appropriately added within a range that does not impair the effects of the present invention, but are not limited thereto.
本発明の美白用皮膚外用剤又は美白用化粧料の形態については特に制限はなく、乳液、クリーム、化粧水、美容液、パック、洗顔料、メーキャップ化粧料等、いずれの形態の皮膚外用剤又は化粧料として調製されたものであってもよい。 There are no particular limitations on the form of the skin whitening external preparation or whitening cosmetic of the present invention, and any form of skin external preparation such as milk, cream, lotion, cosmetic liquid, pack, face wash, makeup cosmetic, etc. It may be prepared as a cosmetic.
以下、実施例により本発明をさらに具体的に説明するが、本発明の範囲は下記の実施例に限定されることはない。
[製造例１]タルトチェリー果実搾汁液の凍結乾燥物の製造
タルトチェリー果実搾汁液の調製例として、果実搾汁液凍結乾燥物の調製方法を以下に示す。なおタルトチェリー果実搾汁液の効果はその調製方法によらない。
タルトチェリー（Ｐｒｕｎｕｓ ｃｅｒａｓｕｓ）の果実を破砕した後、種子を除去し圧搾した。これを加熱濃縮後、ろ過をして得られたろ液をタルトチェリー果実搾汁液とした。このときのタルトチェリー果実搾汁液の乾燥固形分は６７％であった。さらに果実搾汁液を凍結乾燥しタルトチェリー果実搾汁液凍結乾燥物を得た。
[製造例２]タルトチェリー果実抽出物の製造
タルトチェリー果実抽出物の調製例として、溶媒抽出による調製方法を以下に示す。なおタルトチェリー果実抽出物の効果はその調製方法によらない。
タルトチェリー（Ｐｒｕｎｕｓ ｃｅｒａｓｕｓ）の果実１０ｇを破砕し、５０ｖｏｌ％１，３−ブチレングリコール溶液１００ｍＬを加え、室温にて３日間抽出を行った後、ろ過してタルトチェリー果実抽出物を得た。
EXAMPLES Hereinafter, although an Example demonstrates this invention further more concretely, the scope of the present invention is not limited to the following Example.
[Production Example 1] Production of freeze-dried tart cherry fruit juice liquid As a preparation example of tart cherry fruit juice liquid, a method for preparing a fruit juice liquid freeze-dried product is shown below. The effect of tart cherry fruit juice is not dependent on the preparation method.
After crushing the fruit of tart cherry (Prunus cerasus), the seeds were removed and pressed. The filtrate obtained by concentrating this by heating and then filtering was used as a tart cherry fruit juice. At this time, the dry solid content of the tart cherry fruit juice was 67%. Furthermore, the fruit juice was freeze-dried to obtain a freeze-dried tart cherry fruit juice.
[Production Example 2] Manufacture of tart cherry fruit extract As a preparation example of tart cherry fruit extract, a preparation method by solvent extraction is shown below. The effect of the tart cherry fruit extract does not depend on the preparation method.
Tart cherry (Prunus cerasus) 10 g fruit was crushed, 50 vol% 1,3-butylene glycol solution 100 mL was added, extracted at room temperature for 3 days, and then filtered to obtain a tart cherry fruit extract.
実施例１
＜美白効果の評価＞
マウス由来のＢ１６メラノーマ培養細胞を使用し、製造例１で得られたタルトチェリー果実搾汁液凍結乾燥物のメラニン生成抑制効果を調べた。具体的には、２枚の６穴プレートに１０％ＦＢＳ含有ＭＥＭ培地を適量とりＢ１６メラノーマ細胞を播種し、３７℃、二酸化炭素濃度５％中にて静置した。翌日、製造例１で得られた果実搾汁液凍結乾燥物を、その最終濃度が０μｇ／ｍＬ(対照)、３０μｇ／ｍＬ、１００μｇ／ｍＬ、３００μｇ／ｍＬとなるように水に溶解して添加し混和した。培養５日目に培地を交換し、再度検体調製液を添加した。翌日、培地を除き、１枚のプレートについて、細胞をリン酸緩衝液にて洗浄した後回収し、Ｂ１６メラノーマ培養細胞の白色化度を以下の基準にて評価した。対照には検体を添加しないものを用意した。
（判定基準）
＋＋：コウジ酸２００μｇ／ｍＬを添加した試料と同程度の美白効果を示す。
＋ ：コウジ酸１００μｇ／ｍＬを添加した試料と同程度の美白効果を示す。
± ：コウジ酸５０μｇ／ｍＬを添加した試料と同程度の美白効果を示す。
− ：対照と同じ黒色を示す。
また残りの１枚のプレートについて、細胞をホルマリン固定後、１％クリスタルバイオレット溶液に添加し染色した。各検体濃度に対する細胞生育率（％）をモノセレーター（オリンパス社製）で測定した。
結果を下記表１に示す。
Example 1
<Evaluation of whitening effect>
Using mouse-derived B16 melanoma cultured cells, the melanin production inhibitory effect of the freeze-dried tart cherry fruit juice obtained in Production Example 1 was examined. Specifically, an appropriate amount of 10% FBS-containing MEM medium was taken in two 6-well plates, seeded with B16 melanoma cells, and allowed to stand at 37 ° C. in a carbon dioxide concentration of 5%. On the next day, the fruit juice lyophilizate obtained in Production Example 1 is dissolved in water and added so that its final concentration is 0 μg / mL (control), 30 μg / mL, 100 μg / mL, and 300 μg / mL. Mixed. On day 5 of the culture, the medium was changed, and the sample preparation solution was added again. On the next day, the medium was removed and the cells were collected after washing with phosphate buffer on one plate, and the degree of whiteness of the cultured B16 melanoma cells was evaluated according to the following criteria. As a control, a sample to which no specimen was added was prepared.
(Criteria)
++: A whitening effect comparable to that of the sample to which 200 μg / mL kojic acid was added is shown.
+: A whitening effect comparable to that of the sample added with 100 μg / mL kojic acid is shown.
±: A whitening effect comparable to that of the sample added with 50 μg / mL kojic acid is shown.
-: Shows the same black color as the control.
For the remaining one plate, cells were fixed with formalin, added to a 1% crystal violet solution and stained. The cell growth rate (%) with respect to each sample concentration was measured with a monocerator (Olympus).
The results are shown in Table 1 below.
表１の結果から、製造例１で調製したタルトチェリー果実搾汁液凍結乾燥物は＋＋という高い美白効果を示すとともに、その高い美白効果を示す濃度において細胞生育率の低下が少ないという安全性に優れた美白剤であることがわかる。
したがって、本発明のタルトチェリー果実搾汁液は美白剤として利用することができる。また、皮膚外用剤や化粧料に配合することでメラニン生成を抑制し、皮膚のシミ、ソバカスを防ぐ皮膚外用剤又は化粧料を作成することができる。
From the results of Table 1, the freeze-dried tart cherry fruit juice prepared in Production Example 1 exhibits a high whitening effect of ++ and is excellent in safety that there is little decrease in cell growth rate at a concentration showing the high whitening effect. It turns out to be a whitening agent.
Therefore, the tart cherry fruit juice of the present invention can be used as a whitening agent. Moreover, the skin external preparation or cosmetics which suppress a melanin production | generation and prevent the skin spot and buckwheat by mixing with skin external preparations and cosmetics can be created.
実施例２：化粧水
（成分） （質量％）
１ グリセリン ５
２ １，３−ブチレングリコール ６．５
３ ポリオキシエチレン（２０Ｅ．Ｏ．）ソルビタン １．２
モノラウリン酸エステル
４ エタノール １２
５ 乳酸 ０．０５
６ 乳酸ナトリウム ０．１
７ アスコルビン酸グルコシド ２
８ 製造例１のタルトチェリー果実搾汁液凍結乾燥物 ０．０００１
９ パラオキシ安息香酸メチル ０．１
１０ 香料 適量
１１ 精製水 残量
Example 2: Lotion (Ingredient) (mass%)
1 Glycerin 5
2 1,3-butylene glycol 6.5
3 Polyoxyethylene (20E.O.) sorbitan 1.2
Monolaurate 4 Ethanol 12
5 Lactic acid 0.05
6 Sodium lactate 0.1
7 Ascorbic acid glucoside 2
8 Freeze-dried tart cherry fruit juice of Production Example 1 0.0001
9 Methyl paraoxybenzoate 0.1
10 Perfume appropriate amount 11 Purified water remaining
（製造方法）
Ａ：成分３、４及び９、１０を混合溶解する。
Ｂ：成分１、２、５〜８及び１１を混合溶解する。
Ｃ：ＡとＢを混合して均一にし、化粧水を得た。
(Production method)
A: Components 3, 4 and 9, 10 are mixed and dissolved.
B: Components 1, 2, 5-8 and 11 are mixed and dissolved.
C: A and B were mixed and uniformed to obtain a skin lotion.
実施例３：乳液（水中油型）
（成分） （質量％）
１ ポリオキシエチレン（１０Ｅ．Ｏ．）ソルビタン １
モノステアレート
２ ポリオキシエチレン（６０Ｅ．Ｏ．）ソルビット ０．５
テトラオレエート
３ グリセリルモノステアレート １
４ ステアリン酸 ０．５
５ ベヘニルアルコール ０．５
６ スクワラン ８
７ アルブチン ３
８ エタノール ５
９ 製造例２のタルトチェリー果実抽出物 ０．００１
１０ 精製水 残量
１１ パラオキシ安息香酸メチル ０．１
１２ カルボキシビニルポリマー ０．２
１３ 水酸化ナトリウム ０．１
１４ ヒアルロン酸 ０．１
１５ 香料 適量
Example 3: Latex (oil-in-water type)
(Ingredient) (mass%)
1 Polyoxyethylene (10E.O.) sorbitan 1
Monostearate 2 Polyoxyethylene (60EO) Sorbit 0.5
Tetraoleate 3 Glyceryl monostearate 1
4 Stearic acid 0.5
5 Behenyl alcohol 0.5
6 Squalane 8
7 Arbutin 3
8 Ethanol 5
9 Tart cherry fruit extract of Production Example 2 0.001
10 Purified water Remaining 11 Methyl paraoxybenzoate 0.1
12 Carboxyvinyl polymer 0.2
13 Sodium hydroxide 0.1
14 Hyaluronic acid 0.1
15 Perfume appropriate amount
（製造方法）
Ａ：成分１０を加熱し、７０℃に保つ。
Ｂ：成分１〜６、８及び１１を加熱混合し、７０℃に保つ。
Ｃ：ＢにＡを加えて混合し、均一に乳化する。
Ｄ：Ｃを冷却後、成分７、９、１２〜１５を加え、均一に混合して乳液を得た。
(Production method)
A: Component 10 is heated and maintained at 70 ° C.
B: Components 1 to 6, 8 and 11 are heated and mixed and kept at 70 ° C.
C: A is added to B, mixed and uniformly emulsified.
D: After cooling C, components 7, 9, and 12 to 15 were added and mixed uniformly to obtain an emulsion.
実施例４：リキッドファンデーション（水中油型クリーム状）
（成分） （質量％）
１ アクリル酸・メタクリル酸アルキル共重合（注１） ０．５
２ トリエタノールアミン １．５
３ 精製水 残量
４ グリセリン ５
５ パラオキシ安息香酸エチル ０．１
６ １，３ブチレングリコール ５
７ 水素添加大豆リン脂質 ０．５
８ 酸化チタン ５
９ ベンガラ ０．１
１０ 黄酸化鉄 １
１１ 黒酸化鉄 ０．０５
１２ ステアリン酸 ０．９
１３ モノステアリン酸グリセリン ０．３
１４ セトステアリルアルコール ０．４
１５ モノオレイン酸ポリオキシエチレン（２０Ｅ．Ｏ．）ソルビタン ０．２
１６ トリオレイン酸ポリオキシエチレン（２０Ｅ．Ｏ．）ソルビタン ０．２
１７ パラメトキシケイ皮酸２―エチルヘキシル ５
１８ 製造例１のタルトチェリー果実搾汁液凍結乾燥物 ０．００１
１９ 香料 ０．０２
（注１）ペミュレンＴＲ−２（ＮＯＶＥＯＮ社製）
Example 4: Liquid foundation (oil-in-water cream)
(Ingredient) (mass%)
1 Acrylic acid / alkyl methacrylate copolymer (Note 1) 0.5
2 Triethanolamine 1.5
3 Remaining purified water 4 Glycerin 5
5 Ethyl paraoxybenzoate 0.1
6 1,3 Butylene glycol 5
7 Hydrogenated soybean phospholipid 0.5
8 Titanium oxide 5
9 Bengala 0.1
10 Yellow iron oxide 1
11 Black iron oxide 0.05
12 Stearic acid 0.9
13 Glycerol monostearate 0.3
14 cetostearyl alcohol 0.4
15 Polyoxyethylene (20E.O.) sorbitan monooleate 0.2
16 Polyoxyethylene trioleate (20E.O.) sorbitan 0.2
17 2-methoxyhexyl paramethoxycinnamate 5
18 Freeze-dried tart cherry fruit juice of Production Example 1 0.001
19 Perfume 0.02
(Note 1) Pemulen TR-2 (manufactured by NOVEON)
（製造方法）
Ａ：成分６〜１１を分散する。
Ｂ：Ａに成分１２〜１７を加え７０℃で均一に混合する。
Ｃ：成分１〜５を７０℃で均一に混合する。
Ｄ：ＣにＢを加え乳化し、室温まで冷却する。
Ｅ：Ｄに成分１８、１９を添加し均一に混合して水中油型クリーム状リキッドファンデーションを得た。
(Production method)
A: Components 6 to 11 are dispersed.
B: Components 12 to 17 are added to A and mixed uniformly at 70 ° C.
C: Components 1 to 5 are mixed uniformly at 70 ° C.
D: B is added to C to emulsify, and cooled to room temperature.
E: Components 18 and 19 were added to D and mixed uniformly to obtain an oil-in-water cream liquid foundation.
実施例５：日焼け止め化粧料（油中水型クリーム状）
（成分） （質量％）
１ モノラウリン酸ポリオキシエチレン（２０Ｅ．Ｏ．）ソルビタン ０．２
２ ポリオキシエチレン（６０Ｅ．Ｏ．）硬化ヒマシ油 ０．１
３ 精製水 残量
４ ジプロピレングリコール １０
５ 硫酸マグネシウム ０．５
６ アスコルビン酸グルコシド ２
７ シリコーン化合物（注２） ３
８ デカメチルシクロペンタシロキサン ２０
９ イソノナン酸イソトリデシル ５
１０ パラメトキシケイ皮酸２−エチルヘキシル ８
１１ 製造例２のタルトチェリー果実抽出物 ０．００１
１２ ジメチルステアリルアンモニウムヘクトライト １．２
（注２）ＫＦ−６０２８（信越化学工業社製）
Example 5: Sunscreen cosmetic (water-in-oil cream)
(Ingredient) (mass%)
1 Polyoxyethylene (20E.O.) sorbitan monolaurate 0.2
2 Polyoxyethylene (60E.O.) hydrogenated castor oil 0.1
3 Remaining purified water 4 Dipropylene glycol 10
5 Magnesium sulfate 0.5
6 Ascorbic acid glucoside 2
7 Silicone compound (Note 2) 3
8 Decamethylcyclopentasiloxane 20
9 Isotridecyl isononanoate 5
10 2-Ethylhexyl paramethoxycinnamate 8
11 Tart cherry fruit extract of Production Example 2 0.001
12 Dimethylstearyl ammonium hectorite 1.2
(Note 2) KF-6028 (manufactured by Shin-Etsu Chemical Co., Ltd.)
（製造方法）
Ａ：成分１〜６を均一に分散する。
Ｂ：成分７〜１２を均一に分散する。
Ｃ：Ｂを攪拌しながら徐々にＡを加えて乳化し、油中水型クリーム状日焼け止め化粧料を得た。
(Production method)
A: Components 1 to 6 are uniformly dispersed.
B: Components 7 to 12 are uniformly dispersed.
C: While stirring B, A was gradually added to emulsify to obtain a water-in-oil cream sunscreen cosmetic.
実施例６：軟膏剤
（成分） （質量％）
１ ステアリン酸 １８
２ セタノール ４
３ 酢酸ＤＬ−α―トコフェロール（注３） ０．２
４ パラオキシ安息香酸メチル ０．１
５ 製造例１のタルトチェリー果実搾汁液凍結乾燥物 ０．１
６ トリエタノールアミン ２
７ グリセリン ５
８ 精製水 残量
（注３）エーザイ社製
Example 6: Ointment (component) (mass%)
1 Stearic acid 18
2 Cetanol 4
3 DL-α-Tocopherol acetate (Note 3) 0.2
4 Methyl paraoxybenzoate 0.1
5 Freeze-dried tart cherry fruit juice of Production Example 1 0.1
6 Triethanolamine 2
7 Glycerin 5
8 Remaining purified water (Note 3) Made by Eisai
（製造方法）
Ａ．成分６〜８を加熱混合し、７５℃に保つ。
Ｂ．成分１〜５を加熱混合し、７５℃に保つ。
Ｃ．ＡにＢを徐々に加え、これを冷却しながら混合し、軟膏剤を得た。
(Production method)
A. Ingredients 6-8 are heat mixed and maintained at 75 ° C.
B. Ingredients 1-5 are heat mixed and maintained at 75 ° C.
C. B was gradually added to A and mixed while cooling to obtain an ointment.
実施例７：ローション剤
（成分） （質量％）
１ ポリオキシエチレン（２０Ｅ．Ｏ．）ソルビタン １．２
モノラウリン酸エステル
２ エタノール ８
３ パラオキシ安息香酸メチル ０．２
４ 製造例２のタルトチェリー果実抽出物 ０．１
５ １，３−ブチレングリコール ６．５
６ グリセリン ２０
７ アラントイン(注４) ０．２
８ 精製水 残量
（注４）メルク社製
Example 7: Lotion agent (ingredient) (mass%)
1 Polyoxyethylene (20E.O.) sorbitan 1.2
Monolaurate 2 Ethanol 8
3 Methyl paraoxybenzoate 0.2
4 Tart cherry fruit extract of Production Example 2 0.1
5 1,3-butylene glycol 6.5
6 Glycerin 20
7 Allantoin (Note 4) 0.2
8 Remaining purified water (Note 4) Merck
（製造方法）
Ａ．成分１〜４を混合溶解する。
Ｂ．成分５〜８を混合溶解する。
Ｃ．ＡとＢを混合して均一にし、ローション剤を得た。
(Production method)
A. Components 1 to 4 are mixed and dissolved.
B. Components 5 to 8 are mixed and dissolved.
C. A and B were mixed and homogenized to obtain a lotion preparation.
実施例８：パック化粧料
（成分） （質量％）
１ ポリビニルアルコール １５
２ グリセリン １０
３ ポリオキシエチレンメチルグルコシド ３
４ トリオクタン酸グリセリル ５
５ グリチルリチン酸ジカリウム ０．１
６ ポリオキシエチレンアルキルエーテルリン酸ナトリウム １
７ エタノール ２０
８ カオリン ２
９ 酸化チタン ２
１０ 乳酸（５０％水溶液） ０．５
１１ 乳酸ナトリウム（５０％水溶液） ０．５
１２ 防腐剤 適量
１３ 香料 適量
１４ 製造例１のタルトチェリー果実搾汁液凍結乾燥物 ０．１
１５ 精製水 残量
Example 8: Pack cosmetic (component) (mass%)
1 Polyvinyl alcohol 15
2 Glycerin 10
3 Polyoxyethylene methyl glucoside 3
4 Glyceryl trioctanoate 5
5 Dipotassium glycyrrhizinate 0.1
6 Sodium polyoxyethylene alkyl ether phosphate 1
7 Ethanol 20
8 Kaolin 2
9 Titanium oxide 2
10 Lactic acid (50% aqueous solution) 0.5
11 Sodium lactate (50% aqueous solution) 0.5
12 Preservative Appropriate amount 13 Fragrance Appropriate amount 14 Tart cherry fruit juice liquid freeze-dried product of Production Example 1 0.1
15 Remaining amount of purified water
（製造方法）
Ａ．成分１〜６及び１５を７０℃で加熱混合し、室温まで冷却する。
Ｂ．Ａに成分７〜１４を添加混合してパック化粧料を得た。
(Production method)
A. Ingredients 1-6 and 15 are heated and mixed at 70 ° C. and cooled to room temperature.
B. Ingredients 7 to 14 were added to A and mixed to obtain a pack cosmetic.
上記各実施例で調製した化粧水、乳液、リキッドファンデーション、日焼け止め化粧料、軟膏剤、ローション剤、パック化粧料は、いずれも美白効果に優れ、これを皮膚に適用することにより、シミ、ソバカスを防ぐなど優れた効果を発揮するものであり、沈殿物等の発生もなく、安定性も良好で感触の優れたものである。 The lotions, emulsions, liquid foundations, sunscreen cosmetics, ointments, lotions, and pack cosmetics prepared in each of the above examples are all excellent in the whitening effect. It exhibits excellent effects such as prevention of precipitation, does not generate precipitates, has good stability, and is excellent in touch.
本発明によれば、皮膚外用剤又は化粧料に容易に配合でき、美白効果が高い美白剤、及びそれを含有した安定性や安全性が良く、高い美白効果のある皮膚外用剤又は化粧料を提供することができる。 According to the present invention, a whitening agent that can be easily blended into a skin external preparation or cosmetic and has a high whitening effect, and a skin external preparation or cosmetic that contains the same, has good stability and safety, and has a high whitening effect. Can be provided.
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| US6194469B1 (en) * | 1998-12-11 | 2001-02-27 | Board Of Trustees Operating Michigan State Univeristy | Method for inhibiting cyclooxygenase and inflammation using cherry bioflavonoids |
| US7863248B2 (en) * | 2003-10-30 | 2011-01-04 | Meiji Seika Kaisha, Ltd. | Tyrosinase activity inhibitor and ameliorant for facial blood flow |
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