JP5580379B2 - 重合体吸着剤及び製造方法 - Google Patents
重合体吸着剤及び製造方法 Download PDFInfo
- Publication number
- JP5580379B2 JP5580379B2 JP2012196376A JP2012196376A JP5580379B2 JP 5580379 B2 JP5580379 B2 JP 5580379B2 JP 2012196376 A JP2012196376 A JP 2012196376A JP 2012196376 A JP2012196376 A JP 2012196376A JP 5580379 B2 JP5580379 B2 JP 5580379B2
- Authority
- JP
- Japan
- Prior art keywords
- polymer
- insulin
- macroporous
- flow resistance
- pressure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/282—Porous sorbents
- B01J20/285—Porous sorbents based on polymers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/261—Synthetic macromolecular compounds obtained by reactions only involving carbon to carbon unsaturated bonds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
- B01J20/28004—Sorbent size or size distribution, e.g. particle size
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
- B01J20/28016—Particle form
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
- B01J20/28057—Surface area, e.g. B.E.T specific surface area
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
- B01J20/28069—Pore volume, e.g. total pore volume, mesopore volume, micropore volume
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
- B01J20/28069—Pore volume, e.g. total pore volume, mesopore volume, micropore volume
- B01J20/28073—Pore volume, e.g. total pore volume, mesopore volume, micropore volume being in the range 0.5-1.0 ml/g
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
- B01J20/28069—Pore volume, e.g. total pore volume, mesopore volume, micropore volume
- B01J20/28076—Pore volume, e.g. total pore volume, mesopore volume, micropore volume being more than 1.0 ml/g
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
- B01J20/28078—Pore diameter
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
- B01J20/28078—Pore diameter
- B01J20/28083—Pore diameter being in the range 2-50 nm, i.e. mesopores
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
- B01J20/28078—Pore diameter
- B01J20/28085—Pore diameter being more than 50 nm, i.e. macropores
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
- B01J20/28095—Shape or type of pores, voids, channels, ducts
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
- B01J20/287—Non-polar phases; Reversed phases
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/305—Addition of material, later completely removed, e.g. as result of heat treatment, leaching or washing, e.g. for forming pores
- B01J20/3064—Addition of pore forming agents, e.g. pore inducing or porogenic agents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/34—Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
- B01J2220/52—Sorbents specially adapted for preparative chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
- B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/89—Inverse chromatography
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S530/812—Peptides or proteins is immobilized on, or in, an organic carrier
- Y10S530/815—Carrier is a synthetic polymer
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Polymerisation Methods In General (AREA)
- Peptides Or Proteins (AREA)
Description
(i)1グラム当たり0.7から2立方センチメートルの全多孔度;
(ii)1グラム当たり0.7から1.9立方センチメートルのオペレイショナルなメソポロシティー(operational mesoporosity);
(iii)2から600ミクロンの平均粒子直径;
(iv)1グラム当たり200から1500平方メートルの表面積;
(v)10バール圧において700から1,800未満の流れ抵抗値及び60バール圧において1,500から7,000未満の流れ抵抗値;及び
(vi)ポリマーについて75から150グラムインシュリン/リッターである総インシュリン容量及びポリマーについて60から150グラムインシュリン/リッターである動的インシュリン容量(dynamic insulin capacity)を有する、ポリマーを提供する。
(a)1グラム当たり400から1000平方メートルの表面積;
(b)1グラム当たり0.9から1.4立方センチメートルのオペレイショナルなメソポロシティー;
(c)10から75ミクロンの平均粒子直径;
(d)10バール圧において700から1,500未満の流れ抵抗値及び60バール圧において1,500から5,000未満の流れ抵抗値;及び
(e)ポリマーについて90から150グラムインシュリン/リッターである総インシュリン容量及びポリマーについて75から150グラムインシュリン/リッターである動的インシュリン容量を有する、上述のマクロポーラスポリマーを提供する。
V=線速度(cm/時)
ΔP=圧力降下(バール)
dp=ポリマーの平均粒子直径(ミクロン)
ミクロポロシティー=20オングストローム単位未満の細孔
メソポロシティー=20から500オングストローム単位の間の細孔
マクロポロシティー=500オングストローム単位より大の細孔
DVB=ジビニルベンゼン(メタ/パラ異性体の混合物)
EVB=エチルビニルベンゼン(メタ/パラ異性体の混合物)
BPO=ベンゾイルペルオキシド
rpm=分当たりの回転数
v/v=容積/容積
w/v=重量/容積
μ=ミクロン
nm=ナノメートル
g/L=グラム/リッター
cm3/g=立法センチメートル/グラム
μl=マイクロリッター
NA=分析せず
本実施例は、本発明のマクロポーラスポリマーの調製を例示している。有機モノマー相は、次の成分を配合することにより1リッタービーカー中で調製された:180gのジビニルベンゼン混合物(80%DVB/20%EVB)、73gオルトキシレン、147gMIBC及び10.6gBPO(75%純度、25%水分)。これらの量は、122%混合ポローゲン(全モノマー基準)、2.0の親水性ポローゲン/疎水性ポローゲン比率及び4.5%のフリーラジカル開始剤、BPO(全モノマー基準)に相当する。この混合物は、窒素雰囲気下で20分撹拌された。
この実施例は、インシュリン結合容量についての本発明のマクロポーラスポリマーの評価を記載している。約5ml容積の試料は、小規模の試験カラム(内径1.0cm×長さ6.3cm)に充填され、水溶液からウシインシュリンの前端吸着(front adsorption)を評価した;この試験は、ポリマーマトリックスが、典型的な使用条件下で標的プローブ分子(例えば、インシュリン)についての迅速な、効率的な物質移動及び高容量を可能にするか否かを測定するために設計された。加えて、約20mlの試料は、大規模試験カラム(内径1.0cm×長さ25cm)に充填され、インシュリンの分別/分離が遂行された;この試験は、ポリマーマトリックスが典型的なプロセス条件下で満足な性能を提供したことを確認するために行われた。
本実施例は、本発明のマクロポーラスポリマーについてそれらの透過率特性、即ち、圧縮抵抗性の評価法を記載している。ポリマーは、それらの「流れ抵抗」又は1/K値(式3を参照)によって特徴付けられる。
本実施例は、酵素処理をした場合及び酵素処理をしない場合における、本発明のマクロポーラスポリマーを調製することの効果を記載している。酵素処理は、ポリマーの表面汚染(例えば、イオン性又は荷電物質)から生じるビーズ間の望ましくない引力のために使用過程で時折おきる、ポリマーの凝集又は集塊を最小限にする。酵素処理を使用する利点は、結果として生じる球状ポリマー粒子が凝集せず、即ち、粒子がお互いに接着して集塊を形成しないことである。酵素処理はポリマービーズ表面から(重合過程で使用される)分散剤及び沈殿防止剤の痕跡を浄化及び除去し、その結果、カラム内で自由に流れ、均一に詰まるポリマービーズを提供し、延いては使用過程での最小の床容積及び低い圧力降下特性に通じるものであると信じられる。
Claims (7)
- (a)50から100重量%の1以上のポリビニル芳香族モノマー及び(b)0から50重量%の1以上のモノ不飽和ビニル芳香族モノマーの重合されたモノマー単位を含むマクロポーラスポリマーであって、
(i)1グラム当たり0.7から2立方センチメートルの全多孔度;
(ii)1グラム当たり0.7から1.9立方センチメートルのオペレイショナルなメソポロシティー;
(iii)2から600ミクロンの平均粒子直径;
(iv)1グラム当たり200から1500平方メートルの表面積;
(v)10バール圧において700から1,800未満の流れ抵抗値及び60バール圧において1,500から7,000未満の流れ抵抗値;及び
(vi)ポリマーについて75から150グラムインシュリン/リッターである総インシュリン容量及びポリマーについて60から150グラムインシュリン/リッターである動的インシュリン容量を有する、ポリマー。 - ポリビニル芳香族モノマーが、1以上のジビニルベンゼン、トリビニルベンゼン、ジビニルトルエン、ジビニルナフタレン、ジビニルアントラセン及びジビニルキシレンから選択される請求項1に記載のポリマー。
- モノ不飽和ビニル芳香族モノマーが、1以上のスチレン及び(C1〜C4)アルキル置換スチレンから選択される請求項1に記載のポリマー。
- ポリマーが,
(a)1グラム当たり400から1000平方メートルの表面積;
(b)1グラム当たり0.9から1.4立方センチメートルのオペレイショナルなメソポロシティー;
(c)10から75ミクロンの平均粒子直径;
(d)10バール圧において700から1,500未満の流れ抵抗値及び60バール圧において1,500から5,000未満の流れ抵抗値;及び
(e)ポリマーについて90から150グラムインシュリン/リッターである総インシュリン容量及びポリマーについて75から150グラムインシュリン/リッターである動的インシュリン容量を有する請求項1に記載のポリマー。 - (a)75から100重量%の1以上のポリビニル芳香族モノマー及び(b)0から25重量%の1以上のモノ不飽和ビニル芳香族モノマーの重合されたモノマー単位を含む請求項1に記載のポリマー。
- ポリマーが、1以上のジビニルベンゼンコポリマー、スチレン‐ジビニルベンゼンコポリマー、ジビニルベンゼン‐エチルビニルベンゼンコポリマー及びスチレン‐エチルビニルベンゼン‐ジビニルベンゼンコポリマーから選択される請求項1に記載のポリマー。
- 混合された生体分子の水性溶液を、2から100センチメートルの内径を有する液体クロマトグラフィーカラム中で請求項1のマクロポーラスポリマーと接触させる混合された生体分子水性溶液の精製方法であって、カラムが10から100バールの圧力で操作される、方法。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US22437300P | 2000-08-11 | 2000-08-11 | |
US60/224373 | 2000-08-11 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2001244111A Division JP5226918B2 (ja) | 2000-08-11 | 2001-08-10 | 重合体吸着剤及び製造方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2012247437A JP2012247437A (ja) | 2012-12-13 |
JP5580379B2 true JP5580379B2 (ja) | 2014-08-27 |
Family
ID=22840394
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2001244111A Expired - Fee Related JP5226918B2 (ja) | 2000-08-11 | 2001-08-10 | 重合体吸着剤及び製造方法 |
JP2012196376A Expired - Lifetime JP5580379B2 (ja) | 2000-08-11 | 2012-09-06 | 重合体吸着剤及び製造方法 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2001244111A Expired - Fee Related JP5226918B2 (ja) | 2000-08-11 | 2001-08-10 | 重合体吸着剤及び製造方法 |
Country Status (7)
Country | Link |
---|---|
US (1) | US6387974B1 (ja) |
EP (1) | EP1179732B2 (ja) |
JP (2) | JP5226918B2 (ja) |
CN (1) | CN1205239C (ja) |
CZ (1) | CZ20012922A3 (ja) |
DE (1) | DE60102327T3 (ja) |
NO (1) | NO332995B1 (ja) |
Families Citing this family (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GR1002662B (el) * | 1996-06-05 | 1997-04-07 | ������� ���������� ����������� ��� �������� ������� ... | Μεθοδος παραγωγης προιοντων που προσροφουν πετρελαιο και οργανικους διαλυτες. |
SE0201623D0 (sv) * | 2002-05-30 | 2002-05-30 | Amersham Biosciences Ab | Macroporous cross-linked polymer particles |
EP1394196A1 (en) * | 2002-08-16 | 2004-03-03 | Rohm And Haas Company | Resin for solid phase synthesis |
DE60322000D1 (de) * | 2002-08-16 | 2008-08-21 | Rohm & Haas | Harze mit niedrigem Leerraum und Verfahren zu deren Herstellung |
US20040063856A1 (en) * | 2002-09-30 | 2004-04-01 | Bohling James Charles | Resin for solid phase synthesis |
US6884829B2 (en) * | 2002-10-18 | 2005-04-26 | Robert L. Albright | Hemocompatible coated polymer and related one-step methods |
AU2002346522A1 (en) * | 2002-11-25 | 2004-06-18 | Varian, Inc. | Irregularly-shaped macroporous copolymer particles and methods of using same |
US6921703B2 (en) * | 2003-05-13 | 2005-07-26 | Texas Instruments Incorporated | System and method for mitigating oxide growth in a gate dielectric |
DK1589045T3 (da) * | 2004-04-20 | 2007-07-23 | Rohm & Haas | Polymeradsorbent og fremgangsmåde til fremstilling og anvendelse heraf |
US20060247361A1 (en) * | 2005-05-02 | 2006-11-02 | Varian, Inc. | Polar functionalized polymer modified porous substrate for solid phase extraction |
US7311825B2 (en) * | 2005-05-02 | 2007-12-25 | Varian, Inc. | Polymer modified porous substrate for solid phase extraction |
US20060266707A1 (en) * | 2005-05-26 | 2006-11-30 | Fisher Jon R | Drying method for macroporous polymers, and method of preparation and use of macroporous polymers made using the method |
WO2007018589A2 (en) * | 2005-07-28 | 2007-02-15 | Bio-Rad Laboratories, Inc. | Separation of proteins based on isoelectric point using solid-phase buffers |
CN101258175B (zh) | 2005-08-03 | 2011-11-16 | 默克专利股份公司 | 亲水交联聚合物 |
CN101875005A (zh) * | 2010-07-15 | 2010-11-03 | 上海市安全生产科学研究所 | 苯吸附材料及其制备方法 |
KR102196230B1 (ko) | 2012-09-17 | 2020-12-29 | 더블유.알. 그레이스 앤드 캄파니-콘. | 크로마토그래피 매질 및 장치 |
AU2014349666B2 (en) * | 2013-11-17 | 2018-03-01 | New Proteintech Inc. | Method of preparing chromatographic materials |
PL3094390T3 (pl) | 2014-01-16 | 2021-12-06 | W.R. Grace & Co. - Conn. | Nośniki do chromatografii powinowactwa i urządzenia do chromatografii |
US10457720B2 (en) | 2014-03-04 | 2019-10-29 | Merck Patent Gmbh | Robust antibody purification |
ES2929099T3 (es) | 2014-05-02 | 2022-11-24 | Grace W R & Co | Material de soporte funcionalizado y métodos de fabricación y uso de material de soporte funcionalizado |
WO2016096071A1 (en) | 2014-12-15 | 2016-06-23 | Merck Patent Gmbh | Target molecule capture from crude solutions |
JP2018517559A (ja) | 2015-06-05 | 2018-07-05 | ダブリュー・アール・グレース・アンド・カンパニー−コーンW R Grace & Co−Conn | 吸着性バイオプロセス清澄化剤並びにその製造及び使用方法 |
JP6884774B2 (ja) | 2015-10-22 | 2021-06-09 | サイトソーベンツ・コーポレーション | 生体液よりタンパク質ベースの毒素とカリウムを除去するための多機能性の血液適合性多孔性ポリマービーズ吸着剤 |
CN109154604B (zh) * | 2016-04-24 | 2023-07-21 | 沃特世科技公司 | 用于对用两亲强碱性部分改性的聚糖进行分析的带电表面反相色谱材料方法 |
CN105854826B (zh) * | 2016-05-05 | 2018-02-09 | 济南大学 | 一种乙烯基苄基三甲基氯化铵改性芦苇杆吸附剂的制备 |
CN105749868B (zh) * | 2016-05-05 | 2018-01-23 | 济南大学 | 一种乙烯基苄基三甲基氯化铵改性碳纤维吸附剂的制备 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4382124B1 (en) * | 1958-07-18 | 1994-10-04 | Rohm & Haas | Process for preparing macroreticular resins, copolymers and products of said process |
JPS5798504A (en) | 1980-12-11 | 1982-06-18 | Mitsubishi Monsanto Chem Co | Production of vinyl polymer |
JP2534979B2 (ja) * | 1984-12-27 | 1996-09-18 | 東ソー株式会社 | 液体クロマトグラフイ−用充てん剤 |
JPS61141704A (ja) * | 1985-11-11 | 1986-06-28 | Mitsubishi Kasei Vinyl Co | ビニル系重合体の製造方法 |
JPH0224303A (ja) * | 1988-07-12 | 1990-01-26 | Sanken Kako Kk | 多孔***又共重合体の製造方法 |
JPH02126155A (ja) * | 1988-11-07 | 1990-05-15 | Hitachi Ltd | 分離カラム |
JPH03168203A (ja) * | 1989-11-29 | 1991-07-22 | Tokyo Organ Chem Ind Ltd | 多孔性架橋共重合体の製造方法 |
JPH03217201A (ja) * | 1990-01-23 | 1991-09-25 | Tokyo Organ Chem Ind Ltd | 多孔性樹脂に依る吸着分離方法 |
US5416124A (en) * | 1994-06-21 | 1995-05-16 | The Dow Chemical Company | Polymeric adsorbents with enhanced adsorption capacity and kinetics and a process for their manufacture |
US5460725A (en) * | 1994-06-21 | 1995-10-24 | The Dow Chemical Company | Polymeric adsorbents with enhanced adsorption capacity and kinetics and a process for their manufacture |
JPH11158230A (ja) * | 1997-11-27 | 1999-06-15 | Arakawa Chem Ind Co Ltd | 水酸基含有多孔質樹脂の製造方法 |
JPH11183460A (ja) * | 1997-12-17 | 1999-07-09 | Tosoh Corp | 液体クロマトグラフィー用充填剤及びその製造方法及び使用法 |
JPH11193310A (ja) * | 1997-12-27 | 1999-07-21 | Arakawa Chem Ind Co Ltd | アルキル基含有多孔質ポリマー、その製造方法及びその用途 |
US6147127A (en) * | 1999-01-07 | 2000-11-14 | Rohm And Haas Company | High surface area adsorbents and methods of preparation |
-
2001
- 2001-07-31 EP EP01306555A patent/EP1179732B2/en not_active Expired - Lifetime
- 2001-07-31 DE DE60102327T patent/DE60102327T3/de not_active Expired - Lifetime
- 2001-08-03 NO NO20013803A patent/NO332995B1/no not_active IP Right Cessation
- 2001-08-07 US US09/923,935 patent/US6387974B1/en not_active Expired - Lifetime
- 2001-08-10 CZ CZ20012922A patent/CZ20012922A3/cs unknown
- 2001-08-10 JP JP2001244111A patent/JP5226918B2/ja not_active Expired - Fee Related
- 2001-08-10 CN CN01141105.8A patent/CN1205239C/zh not_active Expired - Fee Related
-
2012
- 2012-09-06 JP JP2012196376A patent/JP5580379B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US20020042487A1 (en) | 2002-04-11 |
NO20013803L (no) | 2002-02-12 |
CN1338480A (zh) | 2002-03-06 |
NO332995B1 (no) | 2013-02-11 |
DE60102327D1 (de) | 2004-04-22 |
JP5226918B2 (ja) | 2013-07-03 |
CZ20012922A3 (cs) | 2002-03-13 |
CN1205239C (zh) | 2005-06-08 |
EP1179732B1 (en) | 2004-03-17 |
DE60102327T2 (de) | 2005-03-17 |
NO20013803D0 (no) | 2001-08-03 |
JP2012247437A (ja) | 2012-12-13 |
EP1179732A2 (en) | 2002-02-13 |
DE60102327T3 (de) | 2012-07-05 |
EP1179732A3 (en) | 2003-06-18 |
JP2002145951A (ja) | 2002-05-22 |
US6387974B1 (en) | 2002-05-14 |
EP1179732B2 (en) | 2012-02-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5580379B2 (ja) | 重合体吸着剤及び製造方法 | |
JP5144535B2 (ja) | マクロ孔質カチオン交換樹脂の製造方法 | |
JP4988574B2 (ja) | マクロ孔質イオン交換樹脂 | |
JP4335168B2 (ja) | ポリマー吸着剤、ならびに調製および使用方法 | |
JP3158186B2 (ja) | アルキル基含有多孔質樹脂、その製造方法及びその用途 | |
WO2009118401A1 (en) | Composite material | |
Zhang et al. | Poly (glycidyl methacrylate–divinylbenzene–triallylisocyanurate) continuous-bed protein chromatography | |
Xu et al. | Preparation and application of monodisperse, highly cross-linked, and porous polystyrene microspheres for dye removal | |
Martel et al. | Sorption of aromatic compounds in water using polymer sorbents containing amino groups | |
Hosoya et al. | Uniform-size hydrophobic polymer-based separation media selectively modified with a hydrophilic external polymeric layer | |
Camli et al. | Cibacron blue F3G-A-attached uniform and macroporous poly (styrene-co-divinylbenzene) particles for specific albumin adsorption | |
US20060237367A1 (en) | Polymeric adsorbent, and method of preparation and use | |
EP3233230B1 (en) | Target molecule capture from crude solutions | |
Suárez et al. | Functionalized glycidyl methacrylate based polymers as stationary phases for protein retention | |
US20060266707A1 (en) | Drying method for macroporous polymers, and method of preparation and use of macroporous polymers made using the method | |
KR100699442B1 (ko) | 거대다공성 중합체의 건조방법, 제조방법 및 그 방법을이용하여 제조된 거대다공성 중합체의 용도 | |
JPH11271294A (ja) | 球状多孔質架橋重合体粒子およびその製造方法 | |
WO2001096556A1 (en) | The use of polymer adsorbent particles in dna separation | |
Gökaltun et al. | Octadecylamine-attached poly (3-chloro-2-hydroxypropyl methacrylate-co-ethylene dimethacrylate) microspheres as a new stationary phase for microbore reversed phase chromatography | |
Zhou et al. | A new method for the synthesis of gigaporous polymer beads | |
JP2004190014A (ja) | 多孔質架橋ポリマーおよび吸着剤、ならびにその製造方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20120906 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20140218 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20140624 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20140710 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5580379 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313531 |
|
R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
EXPY | Cancellation because of completion of term |