JP5551534B2 - 根瘤病抵抗性を有するブラシカ・オレラセア植物 - Google Patents
根瘤病抵抗性を有するブラシカ・オレラセア植物 Download PDFInfo
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- JP5551534B2 JP5551534B2 JP2010161513A JP2010161513A JP5551534B2 JP 5551534 B2 JP5551534 B2 JP 5551534B2 JP 2010161513 A JP2010161513 A JP 2010161513A JP 2010161513 A JP2010161513 A JP 2010161513A JP 5551534 B2 JP5551534 B2 JP 5551534B2
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/20—Brassicaceae, e.g. canola, broccoli or rucola
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
- A01H1/045—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection using molecular markers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Mycology (AREA)
- Immunology (AREA)
- Physiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Description
・var. acephala DC.(ケール)
・var. albiflora Sun [= B. alboglabra](中国ケール)
・var. alboglabra [= B. alboglabra](中国ケール)
・var. botrytis L.(カリフラワー、ヘディング・ブロッコリ)
・var. capitata L.(キャベツ)
・var. chinensis Prain(タイサイ)
・var. fimbriata Mill. (キッチン・ケール)
・var. fruticosa metz. (サウザンド・ヘッデッド・ケール)
・var. gemmifera DC. (芽キャベツ)
・var. gongylodes L. (コールラビ)
・var. italica Plenck. (ブロッコリ、カラブレーゼ)
・var. sabauda L. (サボイキャベツ)
・var. sabellica(コラード)
・var. tronchuda L.H. Bailey(トロンチューダ・キャベツ)
・var. costata(ポルトガル・キャベツ)
・var. medullosa(マロー・ステム・ケール)
・var. pamifolia(ケール、ジャージー・ケール)
・var. ramosa(サウザンド・ヘッデッド・ケール)
・var. selensia(ケール)。
0 = 瘤がなく、健康な根系
1 = 側根に小さな瘤が1〜2個あり、根が幾分か茶色くなっていることがある
2 = 側根に小さな瘤が数個あり、主根がわずかに厚みを増していることがある
3 = 主根の大部分が厚くなっている
4 = 主根が極めて厚くなって瘤のある側根と癒着しているが、それでも正常な根がいくらか存在している
5 = 根が1つの塊になり、正常な根が存在していない
1 = 根に瘤が見られない
2 = 側根に瘤が1個
3 = 側根に瘤が数個(植物は健康)
4 = 主根がわずかに瘤になり、側根に小さな瘤が数個
5 = 主根が中程度に瘤になり、側根に小さな瘤が多数と大きな瘤が数個
6 = 主根がひどく瘤になり、側根に大きな瘤が多数
7 = ひどく瘤になり、健康な根がわずかに残る
8 = ひどく瘤になり、健康な根がほとんど残っていない
9 = ひどく瘤になり、健康な根が存在していない
- プライマー(10μl):1.0μl
- dNTP(2.5mM): 2.0μl
- 白金Taq緩衝液 10×:2.5μl(200mMのトリス-HCl、pH8.4と500mMのKClからMgCl2を除去したもの)
- MgCl2(50mM): 0.75μl
- 白金Taq(BRL/ライフ社): 0.2μl(5U/μl)
- 減菌水: μl(DNAをどれだけの量使用するかによって異なる)
最終体積が25μl
- ランプ 0:00 94℃ 0.10
- ランプ 0:00 36℃ 0.30 1サイクル、PCRサイクル(パーキン・エルマー9600)
- ランプ 0:45 72℃ 1.05
3' 94℃
- ランプ 0:00 94℃ 0.10
- ランプ 0:00 36℃ 0.30 40サイクル、PCRサイクル(パーキン・エルマー9600)
- ランプ 0:45 72℃ 1.05
- 5' 72℃
Claims (12)
- 一遺伝子性で優性な登録番号NCIMB 41134としてNCIMBに寄託されたCFL667系に存在する根瘤病抵抗性を有するB.オレラセア植物を作る方法であって、
a)前記根瘤病抵抗性を有するB.ラパ植物を取得するステップと;
b)そのB.ラパ植物をB.オレラセア植物と交配させるステップであって、雌親としてのブロッコリ(B.オレラセア var. italica)と雄親としてのB.ラパの間で実施するステップと;
c)ステップb)の交配により得られた胚を救出するステップと;
d)ステップc)の胚から植物を再生させるステップと;
e)ステップd)の植物の中から根瘤病抵抗性を有するものを選択するステップと;
f)ステップe)で得られた植物をB.オレラセア植物と戻し交配するステップを含む方法。 - エリートB.オレラセア同系交配植物に抵抗性を移入するステップをさらに含む、請求項1に記載の方法。
- 上記同系交配植物を別のB.オレラセア同系交配植物と交配させてハイブリッドを作るステップをさらに含む、請求項2に記載の方法。
- 上記抵抗性が、根瘤病抵抗性を有するB.ラパ植物から得られる、請求項1に記載の方法。
- 上記B.オレラセア植物が、上記抵抗性に対してホモ接合性である、請求項1〜4のいずれか1項に記載の方法。
- 上記B.オレラセア植物が、上記抵抗性に対してヘテロ接合性である、請求項1〜4のいずれか1項に記載の方法。
- 上記B.オレラセア植物が、同系交配体または二ゲノム性半数体である、請求項1〜6のいずれか1項に記載の方法。
- 上記B.オレラセア植物がハイブリッドである、請求項1〜7のいずれか1項に記載の方法。
- 上記B.オレラセア植物が、細胞質雄性不稔である、請求項7または8に記載の方法。
- 請求項1〜9のいずれか1項に記載の方法によって得られるB.オレラセア植物。
- 請求項10に記載の植物の種子。
- 請求項10に記載の植物の一部であって、その部分が、花粉、胚珠、胚のいずれかであるもの。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0217406.8 | 2002-07-26 | ||
GBGB0217406.8A GB0217406D0 (en) | 2002-07-26 | 2002-07-26 | Disease resistant brassicas |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2004525312A Division JP2005533520A (ja) | 2002-07-26 | 2003-07-25 | 根瘤病抵抗性を有するブラシカ・オレラセア植物 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2010284166A JP2010284166A (ja) | 2010-12-24 |
JP5551534B2 true JP5551534B2 (ja) | 2014-07-16 |
Family
ID=9941201
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2004525312A Withdrawn JP2005533520A (ja) | 2002-07-26 | 2003-07-25 | 根瘤病抵抗性を有するブラシカ・オレラセア植物 |
JP2010161513A Expired - Lifetime JP5551534B2 (ja) | 2002-07-26 | 2010-07-16 | 根瘤病抵抗性を有するブラシカ・オレラセア植物 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
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JP2004525312A Withdrawn JP2005533520A (ja) | 2002-07-26 | 2003-07-25 | 根瘤病抵抗性を有するブラシカ・オレラセア植物 |
Country Status (13)
Country | Link |
---|---|
US (4) | US20050262583A1 (ja) |
EP (2) | EP2302061B1 (ja) |
JP (2) | JP2005533520A (ja) |
KR (2) | KR20130090427A (ja) |
CN (1) | CN1678747B (ja) |
AU (1) | AU2003250175B2 (ja) |
BR (2) | BRPI0312962B1 (ja) |
CA (1) | CA2523011C (ja) |
GB (1) | GB0217406D0 (ja) |
MX (1) | MXPA05001010A (ja) |
PL (1) | PL374843A1 (ja) |
RU (1) | RU2369090C2 (ja) |
WO (1) | WO2004013334A1 (ja) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100876971B1 (ko) * | 2007-04-13 | 2009-01-09 | 이관호 | 식물 신품종 로얄채 및 그 육종방법 |
JP2009183224A (ja) * | 2008-02-07 | 2009-08-20 | Mie Prefecture | 病害抵抗性種子繁殖型イチゴ品種の一代雑種育種法 |
EP2308285A1 (en) | 2009-09-22 | 2011-04-13 | Syngenta Participations AG | Brassica oleracea plants resistant to Albugo candida |
JP6138682B2 (ja) * | 2010-05-12 | 2017-05-31 | アグリジェネティクス,インコーポレイテッド | 形質遺伝子移入のためのトウモロコシにおける褐色中肋−3遺伝子特異的マーカーの使用 |
US9516823B2 (en) * | 2010-11-29 | 2016-12-13 | Bejo Zaden B.V. | Plasmodiophora brassicae-resistant Brassica plant, seeds and plant parts thereof and methods for obtaining the same |
US8754290B2 (en) | 2011-02-09 | 2014-06-17 | Dow Agrosciences, Llc | HO/LL canola with resistance to clubroot disease |
CN104185684B (zh) * | 2011-09-27 | 2017-08-01 | 陶氏益农公司 | 对根肿病具有抗性的ho/ll芸苔 |
EP2745679A1 (en) * | 2012-12-21 | 2014-06-25 | Rijk Zwaan Zaadteelt en Zaadhandel B.V. | Brassica plants resistant to clubroot |
KR101483595B1 (ko) | 2013-04-22 | 2015-01-16 | 대한민국 | 양배추 뿌리혹병 저항성 품종을 식별하기 위한 snp 마커 및 그의 용도 |
WO2015038469A1 (en) * | 2013-09-10 | 2015-03-19 | Dow Agrosciences Llc | Molecular markers for blackleg resistance gene rlm2 in brassica napus and methods of using the same |
US10793918B2 (en) * | 2013-09-10 | 2020-10-06 | Dow Agrosciences Llc | Molecular markers for blackleg resistance in canola and methods of using the same |
CN106011134B (zh) * | 2016-06-22 | 2018-10-19 | 沈阳农业大学 | 大白菜抗根肿病CRb基因的共分离分子标记TCR540、引物及应用 |
TW201920684A (zh) * | 2017-08-17 | 2019-06-01 | 日商莎卡達種子股份有限公司 | 甘藍(Brassica oleracea)植物之異型檢出法 |
KR102139804B1 (ko) | 2018-07-30 | 2020-07-31 | 재단법인 전남생물산업진흥원 | 폴리페놀 및 플라보노이드 함량이 증가된 붉은 배추 추출물 제조방법 |
KR20200090566A (ko) | 2019-01-21 | 2020-07-29 | 재단법인 전남생물산업진흥원 | 붉은 배추를 이용한 붉은 배추 발효 식초의 제조방법 |
US20220201954A1 (en) * | 2019-02-15 | 2022-06-30 | BASF Agricultural Solutions Seed US LLC | Clubroot resistant brassica plants |
CN110999779B (zh) * | 2019-12-13 | 2023-01-20 | 西北农林科技大学 | 利用聚合育种和生物技术合成抗根肿病甘蓝型油菜的方法 |
CN110972931A (zh) * | 2019-12-17 | 2020-04-10 | 山西省农业科学院蔬菜研究所 | 一种抗病耐抽薹春白菜的选育方法 |
CN116472954A (zh) * | 2023-05-09 | 2023-07-25 | 江苏省农业科学院 | 一种创制抗根肿病甘蓝纯系的方法 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5254802A (en) | 1987-12-17 | 1993-10-19 | Sietske Hoekstra | Male sterile brassica plants |
JP2730670B2 (ja) * | 1995-10-26 | 1998-03-25 | 農林水産省 野菜・茶業試験場長 | 根こぶ病抵抗性ハクサイの選抜に用いる合成オリゴヌクレオチド |
AU3604100A (en) * | 1999-03-15 | 2000-10-04 | Pioneer Hi-Bred International, Inc. | Methods for enhancing the disease resistance of plants |
KR100490483B1 (ko) * | 2001-02-09 | 2005-05-17 | 충남대학교산학협력단 | 무사마귀병 내성 식물의 효과적 선발을 위한 유전자 마커,프라이머 키트 및 무사마귀병 내성 식물의 선발 방법 |
WO2003000898A1 (en) * | 2001-06-22 | 2003-01-03 | Syngenta Participations Ag | Plant genes involved in defense against pathogens |
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2002
- 2002-07-26 GB GBGB0217406.8A patent/GB0217406D0/en not_active Ceased
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2003
- 2003-07-25 EP EP10176191.4A patent/EP2302061B1/en not_active Expired - Lifetime
- 2003-07-25 EP EP03766293.9A patent/EP1525317B1/en not_active Expired - Lifetime
- 2003-07-25 RU RU2005105563/13A patent/RU2369090C2/ru active
- 2003-07-25 WO PCT/EP2003/008197 patent/WO2004013334A1/en active Application Filing
- 2003-07-25 MX MXPA05001010A patent/MXPA05001010A/es active IP Right Grant
- 2003-07-25 CA CA2523011A patent/CA2523011C/en not_active Expired - Lifetime
- 2003-07-25 US US10/522,094 patent/US20050262583A1/en not_active Abandoned
- 2003-07-25 KR KR1020137020547A patent/KR20130090427A/ko not_active Application Discontinuation
- 2003-07-25 BR BRPI0312962-4A patent/BRPI0312962B1/pt not_active IP Right Cessation
- 2003-07-25 PL PL03374843A patent/PL374843A1/xx unknown
- 2003-07-25 AU AU2003250175A patent/AU2003250175B2/en not_active Expired
- 2003-07-25 BR BR0312962-4A patent/BR0312962A/pt active IP Right Grant
- 2003-07-25 JP JP2004525312A patent/JP2005533520A/ja not_active Withdrawn
- 2003-07-25 CN CN038207362A patent/CN1678747B/zh not_active Expired - Lifetime
- 2003-07-25 KR KR1020057001468A patent/KR20050026012A/ko active Search and Examination
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2009
- 2009-10-06 US US12/573,963 patent/US8013209B2/en not_active Expired - Fee Related
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2010
- 2010-07-16 JP JP2010161513A patent/JP5551534B2/ja not_active Expired - Lifetime
-
2011
- 2011-05-24 US US13/114,176 patent/US9295207B2/en not_active Expired - Fee Related
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2016
- 2016-03-01 US US15/057,684 patent/US10017780B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
AU2003250175B2 (en) | 2008-05-08 |
AU2003250175A1 (en) | 2004-02-23 |
US20110231951A1 (en) | 2011-09-22 |
JP2010284166A (ja) | 2010-12-24 |
US8013209B2 (en) | 2011-09-06 |
BR0312962A (pt) | 2005-06-14 |
US20160177331A1 (en) | 2016-06-23 |
KR20050026012A (ko) | 2005-03-14 |
EP1525317A1 (en) | 2005-04-27 |
US10017780B2 (en) | 2018-07-10 |
CN1678747A (zh) | 2005-10-05 |
US20100037343A1 (en) | 2010-02-11 |
EP1525317B1 (en) | 2019-12-25 |
BRPI0312962B1 (pt) | 2021-04-13 |
US20050262583A1 (en) | 2005-11-24 |
KR20130090427A (ko) | 2013-08-13 |
GB0217406D0 (en) | 2002-09-04 |
MXPA05001010A (es) | 2005-05-16 |
PL374843A1 (en) | 2005-11-14 |
US9295207B2 (en) | 2016-03-29 |
CA2523011C (en) | 2014-07-08 |
CN1678747B (zh) | 2010-05-26 |
JP2005533520A (ja) | 2005-11-10 |
RU2005105563A (ru) | 2005-11-20 |
RU2369090C2 (ru) | 2009-10-10 |
EP2302061A1 (en) | 2011-03-30 |
EP2302061B1 (en) | 2022-09-21 |
CA2523011A1 (en) | 2004-02-12 |
WO2004013334A1 (en) | 2004-02-12 |
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