JP5415789B2 - Fluorous condensing agent, method for separating fluorine component - Google Patents
Fluorous condensing agent, method for separating fluorine component Download PDFInfo
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Description
本発明は、フルオラス縮合剤と、フッ素成分の分離方法に関する。更に詳しくは本発明は、向山縮合剤あるいは向山試薬として知られる縮合剤に対して特定のフッ素量のフルオラスタグ（パーフルオロ炭化水素基）を付加してなるフルオラス縮合剤と、このフルオラス縮合剤を用いて縮合反応を行うことにより、反応後、フルオラス縮合剤に由来するフッ素成分を簡易かつ低コストに分離できるようにしたフッ素成分の分離方法に関する。 The present invention relates to a fluorous condensing agent and a method for separating a fluorine component. More specifically, the present invention relates to a fluorous condensing agent obtained by adding a fluorous tag (perfluorohydrocarbon group) having a specific fluorine amount to a condensing agent known as a Mukaiyama condensing agent or Mukaiyama reagent, and this fluorous condensing agent. The present invention relates to a method for separating a fluorine component, which allows a fluorine component derived from a fluorous condensing agent to be easily and inexpensively separated after the reaction by performing a condensation reaction.
脱水縮合反応は様々なエステル結合やペプチド（タンパク質）のアミド結合の形成に用いられる最も重要な合成反応の一つである。このような縮合反応用の試薬として、「向山縮合剤（向山試薬）」が従来から良く知られている。 The dehydration condensation reaction is one of the most important synthetic reactions used for forming various ester bonds and amide bonds of peptides (proteins). As a reagent for such a condensation reaction, “Mukoyama condensing agent (Mukoyama reagent)” has been well known.
向山縮合剤は、後述する非特許文献１等において報告されたものであって、具体的には、例えば下記の「化１」式に示すようなヨウ化N-メチル-2-クロロピリジニウムを指すが、広義にはN-アルキル-2-ハロピリジニウム塩まで含めることができる。 The Mukaiyama condensing agent was reported in Non-Patent Document 1 and the like described later, and specifically refers to, for example, N-methyl-2-chloropyridinium iodide as shown in the following “Chemical Formula 1” formula. However, in a broad sense, N-alkyl-2-halopyridinium salts can be included.
ところで「化３」式に示す改良型縮合剤は有利なものではあるが、なお重要な改良の余地を残していた。即ち、ライトフルオラス向山縮合剤を使用した場合、反応終了後にこれを粗反応物から分離・除去するには、ＦＳＰＥ（Fluorous Solid Phase Extraction）による液相−固相分離が必要であり、そのため、高価なフルオラスシリカゲルを用いる必要があった。 By the way, although the improved condensing agent represented by the formula 3 is advantageous, it still leaves significant room for improvement. That is, when a light fluorous Mukaiyama condensing agent is used, liquid phase-solid phase separation by FSPE (Fluorous Solid Phase Extraction) is required to separate and remove this from the crude reaction product after completion of the reaction. It was necessary to use a new fluorosilica gel.
そこで本発明は、反応時においてはライトフルオラス向山縮合剤の利点を確保し、縮合反応終了後においては、高価なフルオラスシリカゲルを利用せずに、フルオラス縮合剤に由来するフッ素成分を粗反応物から簡易に分離できるようにすることを、解決すべき技術的課題とする。 Therefore, the present invention ensures the advantages of the light fluorous Mukaiyama condensing agent at the time of reaction, and after the completion of the condensation reaction, without using expensive fluorous silica gel, the fluorine component derived from the fluorous condensing agent is removed from the crude reaction product. It is a technical problem to be solved to enable easy separation.
（第１発明の構成）
上記課題を解決するための本願第１発明の構成は、N-アルキル-2-ハロピリジニウム塩におけるアルキル基をパーフルオロ炭化水素基に置換してなる化合物であって、前記パーフルオロ炭化水素基中のフッ素量が前記化合物の分子量の４０〜６０重量％を占めるものである、フルオラス縮合剤である。
(Configuration of the first invention)
The structure of the first invention of the present application for solving the above problems is a compound obtained by substituting an alkyl group in a N-alkyl-2-halopyridinium salt with a perfluorohydrocarbon group, wherein the perfluorohydrocarbon group Is a fluorous condensing agent that accounts for 40 to 60% by weight of the molecular weight of the compound.
上記の第１発明に係るフルオラス縮合剤において、N-アルキル-2-ハロピリジニウム塩は、「向山縮合剤」あるいは「向山試薬」として知られる縮合剤である。パーフルオロ炭化水素基とは直鎖構造又は分岐鎖構造のパーフルオロアルキル基又はパーフルオロアルケニル基を意味するが、より好ましくは直鎖構造のパーフルオロアルキル基を意味する。このパーフルオロ炭化水素基を、以下、「フルオラスタグ」とも呼ぶ。 In the fluorous condensing agent according to the first invention, the N-alkyl-2-halopyridinium salt is a condensing agent known as “Mukoyama condensing agent” or “Mukoyama reagent”. The perfluorohydrocarbon group means a perfluoroalkyl group or a perfluoroalkenyl group having a straight chain structure or a branched structure, and more preferably a perfluoroalkyl group having a straight chain structure. Hereinafter, this perfluorohydrocarbon group is also referred to as “fluorous tag”.
第１発明に該当する代表的なフルオラス縮合剤として、例えば、ＴｆＯ−を対イオンとするN-メチル-2-クロロピリジニウム塩のメチル基を、直鎖状パーフルオロアルキル基である「−Ｃ１０Ｆ２１」で置換したものを挙げることができる（下記の「化４」参照）。この「化４」に示すフルオラス縮合剤においては、合計フッ素量が化合物分子量の約４９重量％を占める。 Representative fluorous condensing agent corresponding to the first invention, for example, TfO - a pair methyl ions to N- methyl-2-chloro-pyridinium salt, a straight-chain perfluoroalkyl group "-C 10 And those substituted with F 21 ”(see“ Chemical Formula 4 ”below). In the fluorous condensing agent shown in “Chemical Formula 4”, the total fluorine amount accounts for about 49% by weight of the compound molecular weight.
（第２発明の構成）
上記課題を解決するための本願第２発明の構成は、第１発明に記載したフルオラス縮合剤を用いて有機溶媒中で基質の縮合反応を行い、縮合反応の終了後、反応溶媒に水を添加することにより、反応中に生成したフルオラス縮合剤由来のフッ素成分を沈殿させて濾過分離する、フッ素成分の分離方法である。
(Configuration of the second invention)
The structure of the second invention of the present application for solving the above-described problem is that a substrate condensation reaction is performed in an organic solvent using the fluorous condensing agent described in the first invention, and water is added to the reaction solvent after the condensation reaction is completed. This is a method for separating a fluorine component, in which a fluorine component derived from a fluorous condensing agent generated during the reaction is precipitated and separated by filtration.
上記の第２発明において、「フルオラス縮合剤由来のフッ素成分」とは、縮合反応の過程で、ハロピリジニウム塩である第１発明のフルオラス縮合剤から生成するピリドン構造体（フルオラスピリドン）である。このピリドン構造体はピリジニウム塩における対イオンを失っている。 In the second invention, the “fluorine component derived from a fluorous condensing agent” is a pyridone structure (fluorus pyridone) produced from the fluorous condensing agent of the first invention which is a halopyridinium salt in the course of the condensation reaction. This pyridone structure has lost the counterion in the pyridinium salt.
（第３発明の構成）
上記課題を解決するための本願第３発明の構成は、前記第２発明に係る方法が以下の（１）及び／又は（２）に該当する、フッ素成分の分離方法である。
（１）前記有機溶媒がN,N-ジメチルホルムアミド（ＤＭＦ）である。
（２）前記縮合反応がアミド化反応又はエステル化反応である。
(Configuration of the third invention)
The configuration of the third invention of the present application for solving the above problem is a method for separating a fluorine component, wherein the method according to the second invention corresponds to the following (1) and / or (2).
(1) The organic solvent is N, N-dimethylformamide (DMF).
(2) The condensation reaction is an amidation reaction or an esterification reaction.
本発明に係るミディアムフルオラス向山縮合剤は、パーフルオロ炭化水素基中のフッ素量が化合物全体の分子量の４０〜６０重量％を占める。このフッ素量は非特許文献３に記載されたライトフルオラス向山縮合剤との対比ではかなり大きいが、縮合剤としての可溶性と反応性に大きな影響を与える程ではなく、実際、ライトフルオラス向山縮合剤とほぼ同等の高い可溶性と反応性を示すことを確認している。 In the medium fluorous Mukaiyama condensing agent according to the present invention, the amount of fluorine in the perfluorohydrocarbon group accounts for 40 to 60% by weight of the total molecular weight of the compound. Although this fluorine amount is considerably large in comparison with the light fluorous Mukaiyama condensing agent described in Non-Patent Document 3, it does not significantly affect the solubility and reactivity as the condensing agent. It has been confirmed that it exhibits almost the same high solubility and reactivity.
本願発明者は、一方で、ミディアムフルオラス向山縮合剤を用いて有機溶媒（具体的にはＤＭＦ）中で縮合反応を行ったとき、反応終了後の粗反応物中に一定量（ＤＭＦに対して２０％）の水を添加するだけで、フルオラス縮合剤由来のフッ素成分（フルオラスピリドン）が沈殿することを見出した。従って、反応終了後に濾過という簡易な手段でフルオラスピリドンを分離・除去することができ、高価なフルオラスシリカゲルを用いるＦＳＰＥを行う必要がない。 On the other hand, the inventor of the present application conducted a condensation reaction in an organic solvent (specifically, DMF) using a medium fluorous Mukaiyama condensing agent. It was found that the fluorine component (fluorus pyridone) derived from the fluorous condensing agent is precipitated only by adding 20% water. Accordingly, after completion of the reaction, fluoropyridone can be separated and removed by a simple means of filtration, and it is not necessary to perform FSPE using expensive fluorosilica gel.
このように有利な現象が起こる理由は、次のように考えられる。即ち、縮合反応の完了後、ミディアムフルオラス向山縮合剤は反応に付随して対イオンを失い、フルオラスピリドンとなって分子量が減少する。その結果、フッ素量は化合物分子量の６２％に増大する。このように、ミディアムフルオラス向山縮合剤は縮合反応の完了後にフッ素量がミディアムフルオラス化合物のカテゴリーを超える。これを「ヘビーフルオラス化合物」と呼ぶ。その結果、可溶性が低下して、反応終了後の粗反応物中に一定量の水を添加するだけで、沈殿するのである。 The reason why such an advantageous phenomenon occurs is considered as follows. That is, after completion of the condensation reaction, the medium fluorous Mukaiyama condensing agent loses the counter ion accompanying the reaction, and becomes a fluoropyridone, resulting in a decrease in molecular weight. As a result, the fluorine content increases to 62% of the compound molecular weight. Thus, the medium fluorous Mukaiyama condensing agent has a fluorine amount exceeding the category of medium fluorous compound after completion of the condensation reaction. This is called “heavy fluorous compound”. As a result, the solubility is lowered, and precipitation occurs only by adding a certain amount of water into the crude reaction product after completion of the reaction.
なお、Ｃ８Ｆ１７のフルオラスタグを付加したライトフルオラス向山縮合剤では、上記と同じプロセスにおいて、縮合反応自体は良好に進行するが、反応終了後に水を添加しても僅かなフルオラスピリドンしか沈殿しなかった。よって、フッ素成分の濾過による分離・除去は困難である。ライトフルオラス向山縮合剤では、縮合反応の完了後にフルオラスピリドンとなっても化合物中のフッ素量が十分に増大しないためであると考えられる。オリジナルの向山試薬（非フルオラスタイプ）を使用した場合にも、上記と同じプロセスにおいて、反応終了後に水を添加してもピリドンの沈殿が認められなかった。 In the light fluorous Mukaiyama condensing agent to which a C 8 F 17 fluorous tag is added, the condensation reaction itself proceeds satisfactorily in the same process as described above, but even if water is added after the reaction is completed, only a little fluoropyridone is precipitated. I did not. Therefore, it is difficult to separate and remove the fluorine component by filtration. In the light fluorous Mukaiyama condensing agent, it is considered that the amount of fluorine in the compound does not increase sufficiently even if it becomes fluorouspyridone after completion of the condensation reaction. Even when the original Mukaiyama reagent (non-fluorus type) was used, no pyridone precipitation was observed in the same process as described above even when water was added after the reaction was completed.
更に、元々ヘビーフルオラスであるフルオラス向山縮合剤を用いる場合には、反応終了後の分離・精製は別としても、そもそも縮合反応時の可溶性と反応性が不足することは、前記した通りである。 Furthermore, as described above, when the fluorous Mukaiyama condensing agent, which is originally heavy fluorous, is used, the solubility and reactivity during the condensation reaction are insufficient in the first place, apart from the separation and purification after the completion of the reaction.
従って、本発明の課題はミディアムフルオラス向山縮合剤を用いなければ解決できない。 Therefore, the problem of the present invention cannot be solved without using a medium fluorous Mukaiyama condensing agent.
次に、本発明の実施形態を、その最良の形態を含めて説明する。 Next, an embodiment of the present invention including the best mode will be described.
〔フルオラス縮合剤〕
本発明に係るフルオラス縮合剤は向山縮合剤（向山試薬）の基本構造であるN-アルキル-2-ハロピリジニウム塩におけるアルキル基をパーフルオロ炭化水素基に置換してなる化合物であって、パーフルオロ炭化水素基中のフッ素量が前記化合物の分子量の４０〜６０重量％を占めるものである。
[Fluorous condensing agent]
A fluorous condensing agent according to the present invention is a compound obtained by substituting an alkyl group in a N-alkyl-2-halopyridinium salt, which is the basic structure of a Mukaiyama condensing agent (Mukayama reagent), with a perfluorohydrocarbon group. The amount of fluorine in the hydrocarbon group occupies 40 to 60% by weight of the molecular weight of the compound.
ここにおいて、N-アルキル-2-ハロピリジニウム塩を構成するハロゲン原子の種類は限定されないが、好ましくは塩素原子である。又、アルキル基との置換によって縮合剤に導入されるパーフルオロ炭化水素基（フルオラスタグ）は、直鎖構造又は分岐鎖構造のパーフルオロアルキル基又はパーフルオロアルケニル基であるが、より好ましくは直鎖構造のパーフルオロアルキル基である。 Here, the type of halogen atom constituting the N-alkyl-2-halopyridinium salt is not limited, but is preferably a chlorine atom. In addition, the perfluorohydrocarbon group (fluorous tag) introduced into the condensing agent by substitution with an alkyl group is a perfluoroalkyl group or a perfluoroalkenyl group having a linear structure or a branched structure, and more preferably a straight-chain structure. A perfluoroalkyl group having a chain structure.
上記のフルオラスタグは、そのフッ素量（フルオラスタグ中の全フッ素原子の合計重量）が前記フルオラス縮合剤の分子量の４０〜６０重量％、より好ましくは４５〜５５重量％を占める。 In the above fluorous tag, the fluorine amount (total weight of all fluorine atoms in the fluorous tag) accounts for 40 to 60% by weight, more preferably 45 to 55% by weight of the molecular weight of the fluorous condensing agent.
ピリジニウム塩であるフルオラス縮合剤の対イオンの種類は特段に限定されないが、フルオラス縮合剤が対イオンを失ってフルオラスピリドンとなった場合に、その分子量に対してフルオラスタグのフッ素量が６０重量％を超えることとなる大きさの対イオンであることが好ましい。対イオンとしては、例えばＴｆＯ−（トリフレートイオン）、ＰＦ６ −（ヘキサフルオロフォスフェートイオン）等が挙げられる
〔フッ素成分の分離方法〕
本発明に係るフッ素成分の分離方法は、上記した本発明のフルオラス縮合剤を用いて有機溶媒中で基質の縮合反応を行い、縮合反応の終了後、反応溶媒に水を添加することにより、反応中に生成したフルオラス縮合剤由来のフッ素成分を沈殿させて濾過分離する方法である。
The type of counter ion of the fluorous condensing agent which is a pyridinium salt is not particularly limited, but when the fluorous condensing agent loses the counter ion to become fluorous pyridone, the fluorine amount of the fluorous tag is 60% by weight with respect to the molecular weight. It is preferable that the counter ion has a size exceeding. Examples of the counter ion include TfO − (triflate ion), PF 6 − (hexafluorophosphate ion) and the like [Method for separating fluorine component]
In the method for separating a fluorine component according to the present invention, a substrate is condensed in an organic solvent using the above-described fluorous condensing agent of the present invention, and water is added to the reaction solvent after completion of the condensation reaction. In this method, the fluorine component derived from the fluorous condensing agent is precipitated and separated by filtration.
上記の縮合反応とは、具体的には脱水縮合反応であり、例えば、アミド化反応又はエステル化反応を好ましく例示することができる。脱水縮合反応の基質に関しては、例えばアミド化反応の場合においては、一方の基質がカルボキシル基を有する有機酸であり、他方の基質がアミノ基を有するアミン化合物である限りにおいて限定されない。例えばエステル化反応の場合においては、一方の基質がカルボキシル基を有する有機酸であり、他方の基質が水酸基を有するアルコール化合物である限りにおいて限定されない。 The above condensation reaction is specifically a dehydration condensation reaction, and for example, an amidation reaction or an esterification reaction can be preferably exemplified. The substrate for the dehydration condensation reaction is not limited as long as, for example, in the case of an amidation reaction, one substrate is an organic acid having a carboxyl group and the other substrate is an amine compound having an amino group. For example, in the case of an esterification reaction, there is no limitation as long as one substrate is an organic acid having a carboxyl group and the other substrate is an alcohol compound having a hydroxyl group.
フルオラス縮合剤を用いて行う基質の縮合反応は、有機溶媒中で行う。有機溶媒の種類は特段に限定されないが、N,N-ジメチルホルムアミド（ＤＭＦ）、アセトニトリル、テトラヒドロフラン等を好ましく例示することができる。 The substrate condensation reaction performed using a fluorous condensing agent is performed in an organic solvent. Although the kind of organic solvent is not specifically limited, N, N- dimethylformamide (DMF), acetonitrile, tetrahydrofuran, etc. can be illustrated preferably.
フルオラス縮合剤の使用量に対する有機溶媒の量の関係については、例えば、フルオラス縮合剤に対してＤＭＦが10〜20vol./wt.とすることができる。又、反応終了後における水の添加量の関係については、例えば、水をＤＭＦに対して0.25 vol./wt.以上添加することができる。 Regarding the relationship between the amount of the organic solvent and the amount of the fluorous condensing agent used, for example, the DMF can be 10 to 20 vol./wt. In addition, regarding the relationship of the amount of water added after completion of the reaction, for example, water can be added in an amount of 0.25 vol./wt.
〔具体的実施形態〕
本発明のフルオラス縮合剤を用いた縮合反応及びフルオラスピリドンの分離過程の実施形態例を、以下の（１）〜(５)によって具体的に説明する。この実施形態例は、図１に示すように、ベンゾイル酸（ＰｈＣＯ２Ｈ）とアニリン（ＰｈＮＨ２）を基質としてアミド化反応を行った事例であって、後述の実施例で示す表１の entry 3に相当する。図１中、番号「１」で示す化合物が本発明のフルオラス縮合剤である。
[Specific Embodiment]
Embodiment examples of the condensation reaction using the fluorous condensing agent of the present invention and the separation process of fluoropyridone will be specifically described by the following (1) to (5). In this embodiment, as shown in FIG. 1, an amidation reaction was carried out using benzoyl acid (PhCO 2 H) and aniline (PhNH 2 ) as substrates, and the entry of Table 1 shown in the examples described later is used. Corresponds to 3. In FIG. 1, the compound indicated by the number “1” is the fluorous condensing agent of the present invention.
（１）ドライDMF8ml（フルオラス縮合剤１に対して16vol./wt.）に、ベンゾイル酸62.8mg(0.51 mmol)、アニリン47ml(0.51 mmol)、トリエチルアミン（Et3N）0.215ml(1.54 mmol)、４−ジメチルアミノピリドン（DMAP）62.8mg(0.51 mmol)を溶解させた。 (1) Dry DMF 8 ml (16 vol./wt. With respect to fluorous condensing agent 1), benzoylic acid 62.8 mg (0.51 mmol), aniline 47 ml (0.51 mmol), triethylamine (Et 3 N) 0.215 ml (1.54 mmol), 4-Dimethylaminopyridone (DMAP) 62.8 mg (0.51 mmol) was dissolved.
（２）この溶液に500mg(0.61 mmol)のフルオラス縮合剤１を室温下に加えた。 (2) 500 mg (0.61 mmol) of fluorous condensing agent 1 was added to this solution at room temperature.
（３）この反応液を室温で１時間攪拌した後、水2ml（フルオラス縮合剤１に対して4vol./wt.）を加え、更に室温で５分間攪拌した後、濾過した。 (3) After stirring this reaction liquid at room temperature for 1 hour, 2 ml of water (4 vol./wt. With respect to the fluorous condensing agent 1) was added, and further stirred at room temperature for 5 minutes, followed by filtration.
（４）濾別された沈殿を２０％水添加DMF10mlで洗浄した後、1.0M塩酸10mlを濾過物に対して加え、次いでジエチルエーテルで抽出した。 (4) The precipitate separated by filtration was washed with 10 ml of 20% DMF-added DMF, 10 ml of 1.0M hydrochloric acid was added to the filtrate, and then extracted with diethyl ether.
（５）有機層を1.0M塩酸と食塩水で再洗浄し、この有機層を硫酸ソーダNa2SO4で乾燥させて有機相の濃縮物を得た。この濃縮物は縮合生成物（ＰｈＣＯＮＨＰｈ）を定量的収量(101mg, 98％の高純度)で含んでいた。一方、濾過後の粗生成物の１H NMRではフルオラスピリドン（図１中、番号「２」で示す化合物）に相当するシグナルは得られなかった。 (5) The organic layer was rewashed with 1.0 M hydrochloric acid and brine, and the organic layer was dried over sodium sulfate Na 2 SO 4 to obtain an organic phase concentrate. This concentrate contained the condensation product (PhCONHPh) in quantitative yield (101 mg, 98% high purity). On the other hand, in 1 H NMR of the crude product after filtration, a signal corresponding to fluoraspyridone (a compound represented by the number “2” in FIG. 1) was not obtained.
〔比較例１〕
上記「具体的実施形態」で用いた本発明のフルオラス縮合剤１に代えて、前記「化３」式に示すライトフルオラスな縮合剤（フルオラスタグの部分が−Ｃ８Ｆ１７である点のみが異なる）を同量用いて、上記の（１）〜(５)のプロセスを全く同様に行った。
[Comparative Example 1]
Instead of the fluorous condensing agent 1 of the present invention used in the above “specific embodiment”, a light fluorous condensing agent represented by the above “formula 3” formula (only the point that the fluorous tag portion is —C 8 F 17). The above processes (1) to (5) were carried out in exactly the same manner using the same amount of (different).
この場合は、縮合反応自体はうまく行くが、フッ素成分の沈殿による分離がうまく行かず、濾過物（濾過残渣）中には僅かなピリドンしか見られなかった。 In this case, the condensation reaction itself was successful, but separation by precipitation of the fluorine component was not successful, and only a small amount of pyridone was found in the filtrate (filter residue).
〔比較例２〕
上記「具体的実施形態」で用いた本発明のフルオラス縮合剤１に代えて、前記「化１」式に示す非フルオラスタイプの向山縮合剤を同量用いて、上記の（１）〜(５)のプロセスを全く同様に行った。
[Comparative Example 2]
Instead of the fluorous condensing agent 1 of the present invention used in the above “specific embodiment”, the same amount of the non-fluorus type Mukoyama condensing agent represented by the formula “Chemical Formula 1” is used, and the above (1) to (5 ) Process was performed in exactly the same way.
この場合は、２０％水添加DMF中にピリドンの沈殿が認められなかった。 In this case, no pyridone precipitation was observed in 20% water-added DMF.
次に本発明の実施例を説明する。本発明の技術的範囲は、以下の実施例によって限定されない。 Next, examples of the present invention will be described. The technical scope of the present invention is not limited by the following examples.
表１の左欄において「Amidation（アミド化反応）」として区分したEntry番号１〜７に係る実施例と、「Esterification（エステル化反応）」として区分したEntry番号８〜１５に係る実施例を、図２のプロセスに従って行った。 Examples relating to Entry numbers 1 to 7 classified as “Amidation (amidation reaction)” in the left column of Table 1 and examples relating to Entry numbers 8 to 15 classified as “Esterification (esterification reaction)” This was done according to the process of FIG.
表１には、各Entry番号ごとにカルボン酸（RCO2H）の「R」基、アミン化合物（R1NH2）の「R1」基、アルコール化合物（R2OH）の「R2」基の種別を示している。 Table 1, "R 2" of the carboxylic acid for each Entry Number (RCO 2 H) of the "R" groups, amine compound "R 1" groups, alcohol compounds (R 1 NH 2) (R 2 OH) Indicates the type of group.
「R」基の種別の表記において、「Me」はメチル基を、「Ph」はフェニル基を、「MeO」はメトキシ基を表す。Entry14及び15においては「RCO2H」として、分子中にヨウ素の環構造を持つBoc-L-tryptophanを用いた。次に、「R1」基の種別の表記において、entry2の「 i Pr」はイソプロピルを表す。「R1NH2」として、Entry4においてはL-バリンメチルエステルを、Entry7においてはフェニルメチルアミンをそれぞれ用いた。Entry7の反応は室温で２時間行った。次に、「R2」基の種別の表記において、entry8の「Bn」はベンジル基を、entry15の「allyl」はアリル基を表す。entry8の反応は室温で０．５時間行った。 In the description of the type of “R” group, “Me” represents a methyl group, “Ph” represents a phenyl group, and “MeO” represents a methoxy group. In Entry 14 and 15, Boc-L-tryptophan having an iodine ring structure in the molecule was used as “RCO 2 H”. Next, in the notation of the type of “R 1 ” group, “ i Pr ” of entry 2 represents isopropyl. As “R 1 NH 2 ”, L-valine methyl ester was used in Entry4 and phenylmethylamine was used in Entry7. The Entry7 reaction was carried out at room temperature for 2 hours. Next, in the notation of the type of “R 2 ” group, “Bn” in entry 8 represents a benzyl group, and “allyl” in entry 15 represents an allyl group. The entry 8 reaction was carried out at room temperature for 0.5 hour.
表１の「Yield(%)」、「Purity(%)」の欄において、各Entry番号ごとに縮合反応生成物であるアミド化合物又はエステル化合物の収量と純度（HPLCにより決定）を示すが、いずれのEntry番号でも目的物を良好な収量、高純度で得ている。entry14及び15のように、分子中にヨウ素の環構造を持つBoc-L-トリプトファンを使用した場合でも、エステル化反応が良好に進行していることが分かる。 In the columns of “Yield (%)” and “Purity (%)” in Table 1, the yield and purity (determined by HPLC) of the amide compound or ester compound as the condensation reaction product are shown for each entry number. The Entry No. also gives the target product in good yield and high purity. As shown in entry 14 and 15, even when Boc-L-tryptophan having an iodine ring structure in the molecule is used, it can be seen that the esterification reaction proceeds well.
本発明によれば、縮合反応を良好に進行させると共に、縮合反応の終了後にはフルオラス縮合剤に由来するフッ素成分を粗反応物から簡易に分離することができる。 According to the present invention, the condensation reaction proceeds well, and after the completion of the condensation reaction, the fluorine component derived from the fluorous condensing agent can be easily separated from the crude reaction product.
Claims (3)
（１）前記有機溶媒がN,N-ジメチルホルムアミドである。
（２）前記縮合反応がアミド化反応又はエステル化反応である。 The method for separating a fluorine component according to claim 2, wherein the method corresponds to the following (1) and / or (2).
(1) The organic solvent is N, N-dimethylformamide.
(2) The condensation reaction is an amidation reaction or an esterification reaction.
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