JP5410782B2 - 人工受精用培地組成物 - Google Patents
人工受精用培地組成物 Download PDFInfo
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- JP5410782B2 JP5410782B2 JP2009037625A JP2009037625A JP5410782B2 JP 5410782 B2 JP5410782 B2 JP 5410782B2 JP 2009037625 A JP2009037625 A JP 2009037625A JP 2009037625 A JP2009037625 A JP 2009037625A JP 5410782 B2 JP5410782 B2 JP 5410782B2
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Description
CaCl2(無水) 200.00mg/L、Fe(NO3)3・9H2O 0.72mg/L、KCl 400.00mg/L、MgSO4(無水) 97.67mg/L、NaCl 6800.00mg/L、NaH2PO4・H2O 140.00mg/L、アデノシン硫酸 10.00mg/L、ATP(2Na塩) 1.00mg/L、アデニル酸 0.20mg/L、コレステロール 0.20mg/L、デオキシリボース 0.50mg/L、D−グルコース 1000.00mg/L、グルタチオン(GSH) 0.05mg/L、グアニン・HCl 0.30mg/L、ヒポキサンチン(Na塩) 0.354mg/L、フェノールレッド 20.00mg/L、リボース 0.50mg/L、酢酸ナトリウム 50.00mg/L、チミン 0.30mg/L、Tween80(登録商標) 20.00mg/L、ウラシル 0.30mg/L、キサンチン(Na塩) 0.344mg/L、DL−アラニン 50.00mg/L、L−アルギニン・HCl 70.00mg/L、DL−アスパラギン酸 60.00mg/L、L−システイン・HCl・H2O 0.11mg/L、L−シスチン・2HCl 26.00mg/L、DL−グルタミン酸・H2O 150.00mg/L、L−グルタミン 100.00mg/L、グリシン 50.00mg/L、L−ヒスチジン・HCl・H2O 21.88mg/L、L−ヒドロキシプロリン 10.00mg/L、DL−イソロイシン 40.00mg/L、DL−ロイシン 120.00mg/L、L−リジン・HCl 70.00mg/L、DL−メチオニン 30.00mg/L、DL−フェニルアラニン 50.00mg/L、L−プロリン 40.00mg/L、DL−セリン 50.00mg/L、DL−トレオニン 60.00mg/L、DL−トリプトファン 20.00mg/L、L−チロシン(2Na塩) 57.88mg/L、DL−バリン 50.00mg/L、アスコルビン酸 0.05mg/L、α−トコフェロールホスフェート(2Na塩) 0.01mg/L、d−ビオチン 0.01mg/L、カルシフェロール 0.10mg/L、p−パントテン酸カルシウム 0.01mg/L、塩化コリン 0.50mg/L、葉酸 0.01mg/L、i−イノシトール 0.05mg/L、メナジオン 0.01mg/L、ナイアシン 0.025mg/L、ナイアシンアミド 0.025mg/L、p−アミノ安息香酸 0.05mg/L、ピリドキサール・HCl 0.025mg/L、ピリドキシン・HCl 0.025mg/L、リボフラビン 0.01mg/L、チアミン・HCl 0.01mg/L、ビタミンA(アセテート) 0.14mg/L、アスタキサンチン(富士化学工業製) 25mg/L。
NaCl 6294.00mg/L、KCl 534.00mg/L、KH2PO4 162.00mg/L、MgCl2(6水和物) 99.60mg/L、CaCl2(2水和物) 251.40mg/L、NaHCO3 2106.00mg/L、L−アルギニン・HCl 21.00mg/L、L−シスチン 12.00mg/L、L−ヒスチジン 8.00mg/L、L−イソロイシン 26.00mg/L、L−ロイシン 26.00mg/L、L−リジン・HCl 36.98mg/L、L−メチオニン 7.50mg/L、L−フェニルアラニン 16.50mg/L、L−トレオニン 24.00mg/L、L−トリプトファン 4.00mg/L、L−チロシン 18.00mg/L、L−バリン 23.50mg/L、L−グルタミン 146.00mg/L、L−アラニン 8.90mg/L、L−アスパラギン(1水和物) 15.00mg/L、L−アスパラギン酸 13.30mg/L、L−グルタミン酸 14.70mg/L、グリシン 7.50mg/L、L−プロリン 11.50mg/L、L−セリン 10.50mg/L、ビルビン酸ナトリウム 55.00mg/L、乳酸ナトリウム 370.00mg/L、ウシ血清アルブミン 3000.00mg/L、ペニシリン100000.00単位/L、ストレプトマイシン 100mg/L、アンホテリシンB 0.25mg/L。
Claims (6)
- アスタキサンチンを含有することを特徴とする人工受精用培地組成物。
- 人工受精が体外受精であることを特徴とする請求項1記載の培地組成物。
- 人工受精後のウシ胚の培養に用いることを特徴とする請求項1又は2記載の培地組成物。
- 人工受精後の胚の培養において、アスタキサンチンを含有する培地を用いることを特徴とするヒト胚を除く胚の培養方法。
- 人工受精が体外受精であることを特徴とする請求項4記載の胚の培養方法。
- 胚がウシ初期胚であることを特徴とする請求項4又は5記載の胚の培養方法。
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JP2009037625A JP5410782B2 (ja) | 2009-02-20 | 2009-02-20 | 人工受精用培地組成物 |
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JP2009037625A JP5410782B2 (ja) | 2009-02-20 | 2009-02-20 | 人工受精用培地組成物 |
Publications (2)
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JP2010187629A JP2010187629A (ja) | 2010-09-02 |
JP5410782B2 true JP5410782B2 (ja) | 2014-02-05 |
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JP2009037625A Active JP5410782B2 (ja) | 2009-02-20 | 2009-02-20 | 人工受精用培地組成物 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103040797B (zh) * | 2013-01-18 | 2014-05-14 | 新乡医学院 | 虾青素在制备防治有机磷农药所致胚胎毒性的药物中的应用 |
US9962350B2 (en) | 2014-07-11 | 2018-05-08 | Sbi Pharmaceuticals Co., Ltd. | Agent for improving normal development rate of fertilized eggs |
CN111718892B (zh) * | 2020-06-29 | 2023-04-18 | 内蒙古大学 | 一种提高牛体外胚胎培养效率和质量的培养液及方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3856422B2 (ja) * | 1999-01-05 | 2006-12-13 | ニプロ株式会社 | 動物胚培養用培地 |
JP2001211781A (ja) * | 2000-02-01 | 2001-08-07 | Mercian Corp | 牛の繁殖障害を防止する方法および該方法に用いる飼料組成物 |
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