JP5358428B2 - アルコールデヒドロゲナーゼを用いた光学活性第二級アルコールのラセミ化方法 - Google Patents
アルコールデヒドロゲナーゼを用いた光学活性第二級アルコールのラセミ化方法 Download PDFInfo
- Publication number
- JP5358428B2 JP5358428B2 JP2009505898A JP2009505898A JP5358428B2 JP 5358428 B2 JP5358428 B2 JP 5358428B2 JP 2009505898 A JP2009505898 A JP 2009505898A JP 2009505898 A JP2009505898 A JP 2009505898A JP 5358428 B2 JP5358428 B2 JP 5358428B2
- Authority
- JP
- Japan
- Prior art keywords
- alcohol dehydrogenase
- alcohol
- optically active
- racemization
- active secondary
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
GC分析
ガスクロマトグラフ:Variant 3900 ガスクロマトグラフ(FID)
カラム:Chrompack Chirasil-DEX CB(25 m x 0.32 mm x 0.25 μm、1.0 bar H2)
b 正および負のe.e.値は、それぞれ(S)および(R)エナンチオマーの過剰に関する。
アルコールのアセチル化(一般的な方法)
誘導体化は、100 μlの無水酢酸/DMAP溶液を加え、30℃/130 rpmで60分間インキュベートすることにより行った。0.5 mlのH20を加えた。
Claims (5)
- 光学活性第二級アルコールを少なくとも1種のE.C. 1.1.1.クラスのアルコールデヒドロゲナーゼと共にインキュベートすることにより光学活性第二級アルコールをラセミ化する方法であって、前記アルコールデヒドロゲナーゼとして2種のアルコールデヒドロゲナーゼの混合物を使用する方法。
- アルコールデヒドロゲナーゼとして微生物アルコールデヒドロゲナーゼを使用する、請求項1に記載の方法。
- アルコールデヒドロゲナーゼとしてラクトバチルス(Lactobacillus)属またはロドコッカス(Rhodococcus)属の微生物由来のアルコールデヒドロゲナーゼを使用する、請求項1に記載の方法。
- 一方のアルコールデヒドロゲナーゼがプレログ特異性(Prelog specificity)を有し、他方のアルコールデヒドロゲナーゼがアンチプレログ特異性(anti-Prelog specificity)を有する、請求項1に記載の方法。
- 有機溶媒の非存在下でインキュベーションを行うことができる、請求項1に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP06112908 | 2006-04-21 | ||
EP06112908.6 | 2006-04-21 | ||
PCT/EP2007/053858 WO2007122182A1 (de) | 2006-04-21 | 2007-04-19 | Verfahren zur racemisierung optisch aktiver sekundärer alkohole unter verwendung von alkoholdehydrogenasen |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2009534023A JP2009534023A (ja) | 2009-09-24 |
JP5358428B2 true JP5358428B2 (ja) | 2013-12-04 |
Family
ID=38235327
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009505898A Expired - Fee Related JP5358428B2 (ja) | 2006-04-21 | 2007-04-19 | アルコールデヒドロゲナーゼを用いた光学活性第二級アルコールのラセミ化方法 |
Country Status (8)
Country | Link |
---|---|
US (1) | US7795004B2 (ja) |
EP (1) | EP2013352B1 (ja) |
JP (1) | JP5358428B2 (ja) |
CN (1) | CN101426921B (ja) |
AT (1) | ATE438729T1 (ja) |
DE (1) | DE502007001258D1 (ja) |
ES (1) | ES2328417T3 (ja) |
WO (1) | WO2007122182A1 (ja) |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4014573C1 (ja) * | 1990-05-07 | 1991-10-10 | Forschungszentrum Juelich Gmbh, 5170 Juelich, De | |
DE10218689A1 (de) * | 2002-04-26 | 2003-11-20 | Degussa | ADH aus Rhodococcus erythropolis |
KR20080072105A (ko) | 2004-08-26 | 2008-08-05 | 화이자 인코포레이티드 | 단백질 티로신 키나제 저해제 중간체의 제조를 위한거울상이성질선택적 생물학적 전환 |
-
2007
- 2007-04-19 ES ES07728317T patent/ES2328417T3/es active Active
- 2007-04-19 CN CN200780013890.8A patent/CN101426921B/zh not_active Expired - Fee Related
- 2007-04-19 AT AT07728317T patent/ATE438729T1/de active
- 2007-04-19 DE DE502007001258T patent/DE502007001258D1/de active Active
- 2007-04-19 WO PCT/EP2007/053858 patent/WO2007122182A1/de active Application Filing
- 2007-04-19 EP EP07728317A patent/EP2013352B1/de not_active Not-in-force
- 2007-04-19 JP JP2009505898A patent/JP5358428B2/ja not_active Expired - Fee Related
- 2007-04-19 US US12/297,804 patent/US7795004B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
WO2007122182A1 (de) | 2007-11-01 |
JP2009534023A (ja) | 2009-09-24 |
ES2328417T3 (es) | 2009-11-12 |
CN101426921B (zh) | 2013-08-14 |
EP2013352B1 (de) | 2009-08-05 |
US20090098623A1 (en) | 2009-04-16 |
EP2013352A1 (de) | 2009-01-14 |
US7795004B2 (en) | 2010-09-14 |
DE502007001258D1 (de) | 2009-09-17 |
CN101426921A (zh) | 2009-05-06 |
ATE438729T1 (de) | 2009-08-15 |
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