JP5345136B2 - 微細加工分離チャネルに試料及びキャリア液を分配する装置 - Google Patents
微細加工分離チャネルに試料及びキャリア液を分配する装置 Download PDFInfo
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- JP5345136B2 JP5345136B2 JP2010508864A JP2010508864A JP5345136B2 JP 5345136 B2 JP5345136 B2 JP 5345136B2 JP 2010508864 A JP2010508864 A JP 2010508864A JP 2010508864 A JP2010508864 A JP 2010508864A JP 5345136 B2 JP5345136 B2 JP 5345136B2
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6004—Construction of the column end pieces
- G01N30/6017—Fluid distributors
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6095—Micromachined or nanomachined, e.g. micro- or nanosize
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/084—Passive control of flow resistance
- B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Dispersion Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Description
Claims (12)
- 表面の一方に凹設され第2の基板体によって覆われる微細加工分離チャネル(5)を保持している第1の基板体を備える、クロマトグラフ分離装置であって、
前記微細加工分離チャネルは、微細加工ピラーの配列が充填される流量分配領域(20)によって先行され及び/又は後行され、前記微細加工ピラーは、前記流量分配領域の軸方向透過性に対する横方向透過性の比が少なくとも2であるように選択される形状、サイズ、及び位置決めパターンを有することを特徴とする、クロマトグラフ分離装置。 - 前記流量分配領域は、前記分離チャネルの幅と等しい実質的に均一な横方向幅を有する、請求項1に記載のクロマトグラフ分離装置。
- 前記微細加工ピラーは、ダイヤモンド状又は楕円状の形状を有する、請求項1又は2に記載のクロマトグラフ分離装置。
- 前記微細加工ピラーは、横方向幅対軸方向幅の比が3/2よりも大きい、請求項1ないし3のいずれか1項に記載のクロマトグラフ分離装置。
- 試料及びキャリア液の供給及び抜き出し用の入口接続孔及び出口接続孔をもつクロマトグラフ分離装置であって、前記微細加工ピラーは、異なるピラーサイズの異なる区域に配置され、該異なる区域は、最小ピラーを含む区域から最大ピラーを含む区域まで実質的に単調な順序で配置され、最大ピラーを含む区域は、前記入口接続孔又は前記出口接続孔の最も近くに配置される、請求項1ないし4のいずれか1項に記載のクロマトグラフ分離装置。
- 最大ピラーを有する前記微細加工ピラーの区域は、ピラーのない開放区域に隣接する、請求項1ないし5のいずれか1項に記載のクロマトグラフ分離装置。
- 前記開放区域は1μm〜20μmの幅を有する、請求項6に記載のクロマトグラフ分離装置。
- 前記微細加工ピラーのサイズ及びピラー間距離は横方向で変わる、請求項1ないし7のいずれか1項に記載のクロマトグラフ分離装置。
- 前記流量分配領域の少なくとも一部は、前記分離チャネルよりも浅い深さを有する、請求項1ないし8のいずれか1項に記載のクロマトグラフ分離装置。
- 前記流量分配領域の少なくとも一部は、前記分離チャネルを保持している表面とは異なる表面上に配置される、請求項1ないし9のいずれか1項に記載のクロマトグラフ分離装置。
- 少なくとも2つの異なる前記流量分配領域が、異なる表面上に設けられ、前記異なる流量分配領域は、異なる液体槽に接続される、請求項1ないし10のいずれか1項に記載のクロマトグラフ分離装置。
- 前記流量分配領域に、1つ又は複数の不透過薄壁が設けられ、該不透過薄壁は、前記流量分配領域を2つ以上のセクションに少なくとも部分的に分割し、且つ平均流れ方向と平行に設けられ、前記セクションは、それぞれ異なる入口チャネル又は出口チャネルに接続される、請求項1ないし11のいずれか1項に記載のクロマトグラフ分離装置。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EPPCT/EP2007/055008 | 2007-05-23 | ||
EP2007055008 | 2007-05-23 | ||
PCT/EP2008/056369 WO2008142160A1 (en) | 2007-05-23 | 2008-05-23 | Device for the distribution of sample and carrier liquid across a micro-fabricated separation channel |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2010528276A JP2010528276A (ja) | 2010-08-19 |
JP2010528276A5 JP2010528276A5 (ja) | 2012-06-21 |
JP5345136B2 true JP5345136B2 (ja) | 2013-11-20 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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JP2010508864A Active JP5345136B2 (ja) | 2007-05-23 | 2008-05-23 | 微細加工分離チャネルに試料及びキャリア液を分配する装置 |
Country Status (5)
Country | Link |
---|---|
US (1) | US8652420B2 (ja) |
JP (1) | JP5345136B2 (ja) |
CN (1) | CN101688854A (ja) |
DK (1) | DK2150809T3 (ja) |
WO (1) | WO2008142160A1 (ja) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2009043057A2 (en) | 2007-09-27 | 2009-04-02 | Massachusetts Institute Of Technology | Cell rolling separation |
EP2213364A1 (en) * | 2009-01-30 | 2010-08-04 | Albert-Ludwigs-Universität Freiburg | Phase guide patterns for liquid manipulation |
EP2583093B1 (en) * | 2010-06-15 | 2016-05-11 | GE Healthcare Bio-Sciences AB | Fluid distributor unit |
EP2718709B1 (en) * | 2011-06-09 | 2016-08-10 | Waters Technologies Corporation | Reducing dispersion due to vias in planar microfluidic separation devices |
KR101911435B1 (ko) * | 2011-09-26 | 2018-10-25 | 삼성전자주식회사 | 유체 제어 장치, 이를 포함하는 필터 및 바이오칩 |
US20140227777A1 (en) * | 2011-09-30 | 2014-08-14 | Brigham And Women's Hospital | Cell sorting by 3d flow and adhesive rolling |
AU2012315950B2 (en) * | 2011-09-30 | 2017-01-19 | The Regents Of The University Of California | Devices and methods for programming fluid flow using sequenced microstructures |
KR101911436B1 (ko) * | 2011-12-13 | 2018-10-25 | 삼성전자주식회사 | 높은 종횡비를 갖는 세포 포획용 필터 |
EP3088892B1 (en) | 2012-01-20 | 2018-07-25 | Ortho-Clinical Diagnostics Inc | Assay device having uniform flow around corners |
US9103468B2 (en) * | 2012-07-30 | 2015-08-11 | University Of South Carolina | Enhanced flow boiling in microchannels by high frequency microbubble-excited and -modulated oscillations |
BE1022314B1 (nl) * | 2013-02-05 | 2016-03-15 | PharmaFluidics N.V. | Chemische reactor inrichting |
EP3396355A1 (en) | 2017-04-27 | 2018-10-31 | Pharmafluidics NV | Lateral detection of fluid properties |
BE1027013B1 (nl) * | 2019-01-31 | 2020-09-01 | Pharmafluidics N V | Filter voor chemische reactoren |
Family Cites Families (11)
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US5427663A (en) * | 1993-06-08 | 1995-06-27 | British Technology Group Usa Inc. | Microlithographic array for macromolecule and cell fractionation |
US6156273A (en) | 1997-05-27 | 2000-12-05 | Purdue Research Corporation | Separation columns and methods for manufacturing the improved separation columns |
JP2001523811A (ja) * | 1997-11-14 | 2001-11-27 | ダイアックス コーポレイション | 液体クロマトグラフィカラム |
EP0977030B1 (en) * | 1998-07-29 | 2001-03-21 | Hewlett-Packard Company | Chip for performing an electrophoretic separation of molecules and method using same |
CA2366913A1 (en) * | 1999-02-25 | 2000-08-31 | Pall Corporation | Chromatography devices and flow distributor arrangements used in chromatography devices |
US6696022B1 (en) * | 1999-08-13 | 2004-02-24 | U.S. Genomics, Inc. | Methods and apparatuses for stretching polymers |
US20050095602A1 (en) * | 2003-11-04 | 2005-05-05 | West Jason A. | Microfluidic integrated microarrays for biological detection |
US20060065528A1 (en) | 2004-02-03 | 2006-03-30 | Gabriel Lopez | Nanostructured devices for separation and analysis |
SE528233C2 (sv) * | 2005-06-20 | 2006-09-26 | Aamic Ab | Metod och medel för att åstadkomma vätsketransport |
US8288151B2 (en) * | 2005-06-29 | 2012-10-16 | Canon Kabushiki Kaisha | Biochemical reaction cassette |
JP4878200B2 (ja) * | 2005-06-29 | 2012-02-15 | キヤノン株式会社 | 生化学反応カセット |
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2008
- 2008-05-23 US US12/601,237 patent/US8652420B2/en active Active
- 2008-05-23 WO PCT/EP2008/056369 patent/WO2008142160A1/en active Application Filing
- 2008-05-23 DK DK08759970.0T patent/DK2150809T3/da active
- 2008-05-23 CN CN200880022752A patent/CN101688854A/zh active Pending
- 2008-05-23 JP JP2010508864A patent/JP5345136B2/ja active Active
Also Published As
Publication number | Publication date |
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WO2008142160A1 (en) | 2008-11-27 |
CN101688854A (zh) | 2010-03-31 |
JP2010528276A (ja) | 2010-08-19 |
DK2150809T3 (da) | 2019-08-05 |
US20100166611A1 (en) | 2010-07-01 |
US8652420B2 (en) | 2014-02-18 |
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