JP5089924B2 - IgM型抗体の精製方法、IgM型抗体認識抗原の吸着材 - Google Patents
IgM型抗体の精製方法、IgM型抗体認識抗原の吸着材 Download PDFInfo
- Publication number
- JP5089924B2 JP5089924B2 JP2006166542A JP2006166542A JP5089924B2 JP 5089924 B2 JP5089924 B2 JP 5089924B2 JP 2006166542 A JP2006166542 A JP 2006166542A JP 2006166542 A JP2006166542 A JP 2006166542A JP 5089924 B2 JP5089924 B2 JP 5089924B2
- Authority
- JP
- Japan
- Prior art keywords
- igm
- type antibody
- antibody
- igm type
- sodium urate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Peptides Or Proteins (AREA)
Description
のみに限定されるものではない。
全血清をリポソーバー(株式会社カネカ製)を充填したLDL除去カラムに通し、LDL除去画分と1M塩化ナトリウム溶液で溶出したLDL画分を得た。LDL除去画分をさらに微顆粒性セルロースの陰イオン交換カラムDE52(Whatman)に通しIgG画分とIgG除去画分を得た。またLDL除去画分にZymosanを加え37℃で30分インキュベートして補体消費画分を得た。
マウスモノクローナル抗体を以下のようにして作成した。ヒト尿細管分泌尿蛋白質に対するIgM型(UP509、UP599)およびIgG1型マウスモノクローナル抗体(UP720)を産生するハイブリドーマ(日本泌尿器科学会雑誌.87巻、1120頁、1996年)を、牛胎児血清を10%含む培養液(RPMI1640)で培養し上清を採取した。
IgM型抗体を産生する3種類のハイブリドーマ(UP70、UP509、UP599)をBALB/cマウスの腹腔内に注射して貯留した腹水を採取した。
Claims (1)
- IgM型抗体含有液を尿酸ナトリウム結晶と接触させることによりIgM型抗体を尿酸ナトリウム結晶に選択的に結合させ、その後にIgM型抗体を尿酸ナトリウム結晶から解離、回収することを特徴とするIgM型抗体の分離精製方法であって、前記分離精製方法は、IgM型抗体含有液をデキストラン硫酸を固定してなる吸着材と接触させる前処理を含むことを特徴とするIgM型抗体の分離精製方法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2006166542A JP5089924B2 (ja) | 2006-06-15 | 2006-06-15 | IgM型抗体の精製方法、IgM型抗体認識抗原の吸着材 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2006166542A JP5089924B2 (ja) | 2006-06-15 | 2006-06-15 | IgM型抗体の精製方法、IgM型抗体認識抗原の吸着材 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2007332080A JP2007332080A (ja) | 2007-12-27 |
JP5089924B2 true JP5089924B2 (ja) | 2012-12-05 |
Family
ID=38931885
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006166542A Active JP5089924B2 (ja) | 2006-06-15 | 2006-06-15 | IgM型抗体の精製方法、IgM型抗体認識抗原の吸着材 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP5089924B2 (ja) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5565723B2 (ja) * | 2009-04-07 | 2014-08-06 | 学校法人北里研究所 | 抗dcdモノクローナル抗体 |
JP2010284607A (ja) * | 2009-06-12 | 2010-12-24 | Toshiba Corp | リン吸着材、及びリン回収システム |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS559036A (en) * | 1978-07-04 | 1980-01-22 | Green Cross Corp:The | Purification of igm |
-
2006
- 2006-06-15 JP JP2006166542A patent/JP5089924B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
JP2007332080A (ja) | 2007-12-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR101569783B1 (ko) | 항체의 정제 방법 | |
JP4831436B2 (ja) | クロマトグラフィーリガンド | |
US10131714B2 (en) | Protein purification using bis-tris buffer | |
US20050272917A1 (en) | Methods for immunoglobulin purification | |
EP2768843B1 (en) | Solid phase for mixed-mode chromatographic purification of proteins | |
JP2017002049A (ja) | タンパク質の精製装置および方法 | |
KR20160005047A (ko) | 항체를 정제하기 위한 연속 다단계 공정 | |
SG162806A1 (en) | Methods of purifying anti a beta antibodies | |
US20190330269A1 (en) | Method for purifying antibodies using pbs | |
JP5089924B2 (ja) | IgM型抗体の精製方法、IgM型抗体認識抗原の吸着材 | |
JP2011036128A (ja) | 抗体製造方法 | |
JP2010070490A (ja) | 抗体の精製方法 | |
FI116569B (fi) | Menetelmä virusinaktivoidun, tekijä-VIII-pitoisen fraktion valmistamiseksi kromatografisilla menetelmillä | |
Bresolin et al. | Evaluation of Iminodiacetic Acid (IDA) as an Ionogenic Group for Adsorption of IgG 1 Monoclonal Antibodies by Membrane Chromatography | |
CN108085358A (zh) | 一种犬细小病毒单克隆抗体IgG2b-Fab片段的制备方法 | |
KR20150084836A (ko) | 벤조나아제 부재 하의 시나지스 분리 방법 | |
US20090264630A1 (en) | Method of separating monomeric protein(s) | |
US20210317163A1 (en) | Compositions And Methods For Simplified High Efficiency Isolation of Proteins | |
JP2006026607A (ja) | アフィニティークロマトグラフィー用吸着体 | |
Kordzangene et al. | Reduction of Purification Time of Polyspecific Equine F (ab´) 2 Antivenom against Scorpion Envenomation | |
JP2017025025A (ja) | タンパク質の精製方法 | |
BRPI0900276A2 (pt) | processo de purificação de imunoglobulina g (igg) a partir do soro ou plasma humano por cromatografia em gel (omega)-aminohexil-agarose ou (omega)-aminodecil- agarose | |
JPH0459797A (ja) | 抗体の精製法 | |
JPH07146280A (ja) | アフィニティクロマトグラフィー用吸着体及びこれを 用いた抗体の分離方法、抗体濃度の測定方法、抗体断 片の精製濃縮方法 | |
JP2010180170A (ja) | 抗体製造方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20090423 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20111004 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20120321 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20120516 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20120911 |
|
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20120912 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20150921 Year of fee payment: 3 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5089924 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313531 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20150921 Year of fee payment: 3 |
|
R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |