JP5053089B2 - 化合物の直接単離および多成分サンプルの分別のための磁性材料の使用 - Google Patents
化合物の直接単離および多成分サンプルの分別のための磁性材料の使用 Download PDFInfo
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Description
この実験は、血漿アルブミンおよびIgG含量を低下させ、それによって問題となっているその他のサンプルタンパク質の質量分光分析を強化するための手段として、磁性粒子ベースのアフィニティークロマトグラフィーを評価するために行った。この手順の概要を、以下の表Iにまとめる。
Claims (12)
- a)鉄、コバルト、およびニッケルからなる群から選択された金属;酸化鉄、硫化鉄、塩化鉄、水酸化第二鉄、および酸化第一第二鉄からなる群から選択された金属化合物;および有機金属化合物を含む少なくとも1つの常磁性粒子を、1種または複数の目標化合物を含むサンプルに添加するステップであって、前記1種または複数の目標化合物は、抗体、ハプテン、酵素、ポリペプチドおよびタンパク質から群から選択され、前記少なくとも1つの常磁性粒子には、1つまたは複数のリガンドまたは官能基が結合されており、前記リガンドまたは官能基は、サンプル中の目標化合物の1種または複数に対してアフィニティーを有するステップと、
b)サンプルのpHを制御して、前記常磁性粒子に対して電荷を結合させるステップと、
c)1つまたは複数のリガンドまたは官能基を、1種または複数の目標化合物に接触させて、複合体を形成するステップと、
d)磁気抽出ブロックにより印加される外部磁場を印加することによって複合体を固定化するステップと、
e)磁場によって固定化されていないサンプルの部分を除去するステップと、
f)磁場を除去して前記複合体を解放するステップと、
g)前記複合体から目標化合物を溶出するステップと、
h)常磁性粒子を固定化するステップと、
i)目標化合物を回収するステップと
を含むことを特徴とするサンプルから目標化合物を単離するための方法。 - 1つまたは複数のリガンドまたは官能基は、少なくとも1つの常磁性粒子と共有結合することを特徴とする請求項1に記載の方法。
- 1つまたは複数のリガンドまたは官能基は、目標化合物に特異的であることを特徴とする請求項1に記載の方法。
- 1つまたは複数のリガンドまたは官能基は、生物学的結合によって結合されることを特徴とする請求項1に記載の方法。
- 生物学的結合は、ストレプトアビジン−ビオチン、アビジン−ビオチン、炭水化物−レクチン、および酵素−酵素阻害剤からなる群から選択されることを特徴とする請求項4に記載の方法。
- a)少なくとも1つの常磁性粒子を、1種または複数の目標化合物を含むサンプルに添加するステップであって、前記少なくとも1つの常磁性粒子には、1つまたは複数のリガンドまたは官能基が結合されており、前記1つまたは複数のリガンドまたは官能基が、前記サンプル中の非目標化合物の1種または複数に対してアフィニティーを有するものであり、前記問題となっていない化合物は抗体、ハプテン、酵素、ポリペプチドおよびタンパク質からなる群から選択されるステップと、
b)サンプルのpHを制御して、前記常磁性粒子に対して電荷を結合させるステップと、
c)リガンドまたは官能基を、前記非目標化合物に接触させて、複合体を形成するステップと、
d)磁気抽出ブロックにより印加される外部磁場を印加することによって複合体を固定化するステップと、
e)磁場によって固定化されていないサンプルの部分を除去するステップであって、このように除去された前記サンプルが、1種または複数の目標化合物を含有するものであるステップと
を含むことを特徴とする1種または複数の目標化合物および非目標化合物を含むサンプルから目標化合物を単離するための方法。 - 1つまたは複数のリガンドまたは官能基は、常磁性粒子と共有結合することを特徴とする請求項6に記載の方法。
- 前記1つまたは複数のリガンドまたは官能基は、前記非目標化合物に特異的であることを特徴とする請求項6に記載の方法。
- 少なくとも1つの常磁性粒子は、鉄、コバルト、およびニッケルからなる群から選択された金属であることを特徴とする請求項6に記載の方法。
- 少なくとも1つの常磁性粒子は、酸化鉄、硫化鉄、塩化鉄、水酸化第二鉄、および酸化第一第二鉄からなる群から選択された鉄化合物であることを特徴とする請求項6に記載の方法。
- 1つまたは複数のリガンドまたは官能基は、生物学的結合により結合されることを特徴とする請求項6に記載の方法。
- 生物学的結合は、ストレプトアビジン−ビオチン、アビジン−ビオチン、炭水化物−レクチン、および酵素−酵素阻害剤からなる群から選択されることを特徴とする請求項11に記載の方法。
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US59811804P | 2004-08-03 | 2004-08-03 | |
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CA2839092C (en) | 2018-04-03 |
CA2839092A1 (en) | 2006-02-16 |
US20060084089A1 (en) | 2006-04-20 |
WO2006017428A2 (en) | 2006-02-16 |
AU2005271688A1 (en) | 2006-02-16 |
WO2006017428A3 (en) | 2006-10-26 |
EP1774334A2 (en) | 2007-04-18 |
EP1774334B1 (en) | 2017-10-04 |
CA2575446A1 (en) | 2006-02-16 |
AU2005271688B2 (en) | 2011-10-06 |
CA2575446C (en) | 2014-03-25 |
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