JP5021408B2 - バイオセンサー - Google Patents
バイオセンサー Download PDFInfo
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- JP5021408B2 JP5021408B2 JP2007248500A JP2007248500A JP5021408B2 JP 5021408 B2 JP5021408 B2 JP 5021408B2 JP 2007248500 A JP2007248500 A JP 2007248500A JP 2007248500 A JP2007248500 A JP 2007248500A JP 5021408 B2 JP5021408 B2 JP 5021408B2
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- Prior art keywords
- hydrophilic polymer
- polymer layer
- substrate
- group
- film
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- IWBOPFCKHIJFMS-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl) ether Chemical compound NCCOCCOCCN IWBOPFCKHIJFMS-UHFFFAOYSA-N 0.000 description 1
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- 229940117841 methacrylic acid copolymer Drugs 0.000 description 1
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- LVHBHZANLOWSRM-UHFFFAOYSA-N methylenebutanedioic acid Natural products OC(=O)CC(=C)C(O)=O LVHBHZANLOWSRM-UHFFFAOYSA-N 0.000 description 1
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- 108010087904 neutravidin Proteins 0.000 description 1
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- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
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- WLAHGGSLQFSJKG-UHFFFAOYSA-N phenyl 2,3-diaminobenzenesulfonate Chemical compound NC1=CC=CC(S(=O)(=O)OC=2C=CC=CC=2)=C1N WLAHGGSLQFSJKG-UHFFFAOYSA-N 0.000 description 1
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- 229920001983 poloxamer Polymers 0.000 description 1
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- 229920000515 polycarbonate Polymers 0.000 description 1
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- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000139 polyethylene terephthalate Polymers 0.000 description 1
- 239000005020 polyethylene terephthalate Substances 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001451 polypropylene glycol Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 125000002112 pyrrolidino group Chemical group [*]N1C([H])([H])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
- 238000002494 quartz crystal microgravimetry Methods 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
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- 238000004062 sedimentation Methods 0.000 description 1
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- 239000000661 sodium alginate Substances 0.000 description 1
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- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
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- 230000001960 triggered effect Effects 0.000 description 1
- 239000013638 trimer Substances 0.000 description 1
- MYPYJXKWCTUITO-LYRMYLQWSA-N vancomycin Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)NC)[C@H]1C[C@](C)(N)[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-N 0.000 description 1
- 229960003165 vancomycin Drugs 0.000 description 1
- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/002—Electrode membranes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Wood Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Photoreceptors In Electrophotography (AREA)
- Holo Graphy (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Description
好ましくは、自己組織化膜が、メルカプト基を有する化合物により形成されたものである。
好ましくは、自己組織化膜は、下記一般式A−1で表される化合物により形成されたものである。
好ましくは、本発明のバイオセンサーは、非電気化学的検出に使用される。
好ましくは、本発明のバイオセンサーは、表面プラズモン共鳴分析に使用される。
本発明のバイオセンサーは、金属基板の表面上に被覆した自己組織化膜の上に、第一の親水性高分子層が固定されているバイオセンサーであって、前記第一の親水性高分子層の上に第二の親水性高分子層が被覆されており、前記第一の親水性高分子層及び前記第二の親水性高分子層の合計の乾燥時の膜厚が20nm以上100nm以下であることを特徴とするバイオセンサーである。
親水性高分子層の乾燥時の膜厚の測定方法は特に限定されず、例えば、AFM、エリプソメトリーなどで評価することができる。具体的には、AFMでの測定法としては、タッピングモードによる塗布/未塗布部分の相対値を膜厚とし、測定を行うことができる。また、エリプソメトリーでは、反射した偏光ビームの相対的な位相の変化を測定することによって膜厚を算出することができる。
本発明において自己組織化膜形成分子とは、金属膜と第一の親水性高分子層とをつなぐ役目を有している。以下、自己組織化膜形成分子を有してなる、自己組織化膜(SAMs)について説明する。
金属基板の表面上に被覆した自己組織化膜の上に固定されている第一の親水性高分子層を形成する親水性高分子としては、カルボキシル基含有合成高分子およびカルボキシル基含有多糖類を用いることが可能である。カルボキシル基含有合成高分子としては、ポリアクリル酸、ポリメタクリル酸、およびこれらの共重合体、例えば特開昭59−53836号第3頁右上第2行目〜第6頁左下第9行目、特開昭59−71048号第3頁左下第1行目〜第5頁左上第3行目に記載されているようなメタクリル酸共重合体、アクリル酸共重合体、イタコン酸共重合体、クロトン酸共重合体、マレイン酸共重合体、部分エステル化マレイン酸共重合、水酸基を有する高分子に酸無水物を付加させたものなどが挙げられる。カルボキシル基含有多糖類は、天然植物からの抽出物、微生物発酵の生産物、酵素による合成物、または化学合成物の何れであってもよく、具体的には、ヒアルロン酸、コンドロイチン硫酸、ヘパリン、デルマタン酸硫酸、カルボキシメチルセルロース、カルボキシエチルセルロース、セロウロン酸、カルボキシメチルキチン、カルボキシメチルデキストラン、カルボキシメチルデンプン等が挙げられる。カルボキシル基含有多糖類は、市販の化合物を用いることが可能であり、具体的には、カルボキシメチルデキストランであるCMD、CMD−L、CMD−D40(名糖産業社製)、カルボキシメチルセルロースナトリウム(和光純薬社製)、アルギン酸ナトリウム(和光純薬社製)、等を挙げることができる。
本発明において第一の親水性高分子層を形成する親水性高分子は、活性エステル化されたカルボキシル基を含有する高分子であることが好ましい。活性エステル化されたカルボキシル基を含有する高分子は、溶液として基板と反応させてもよく、また、スピンコート等の手法を用いて基板上の薄膜を形成させた状態で反応させてもよい。好ましくは、薄膜を形成させた状態での反応である。
本発明において、第一の親水性高分子層の上には直接第二の親水性高分子層が形成されている。ここで「直接」とは、第一の親水性高分子層と第二の親水性高分子層との間に生理活性物質などを有していないことを指す。本発明において、第二の親水性高分子層は、酸素などが透過し、自己組織化膜が酸化され、剥がれなどが生じることを抑止する。本発明における「第一の親水性高分子層の上に被覆されている第二の親水性高分子層」は、自己組織化膜の酸化、剥離を抑制する目的で使用することができる。また、先述の通り、第一の親水性高分子層のみで乾燥時に20nm以上100nm以下の膜厚とすることでも、上記効果を得ることができる。
生理活性物質は、第二の親水性高分子層の剥離後、第一の親水性高分子層上に固定される。
上記の方法により基板に設置された、第一の親水性高分子層を形成しているカルボキシル基を含有する高分子は、公知の方法、例えば、水溶性カルボジイミドである1-(3-Dimethylaminopropyl)-3 ethylcarbodiimide(EDC)単独、又は、EDCとN-Hydroxysuccinimide(NHS)により活性化され、アミノ基を有する生理活性物質を固定化することが可能となる。本発明のバイオセンサーには、上記のようにして生理活性物質を固定したものでもよいし、あるいは生理活性物質を固定しない状態のものでもよい。生理活性物質を固定しない状態のものを使用する場合は、使用の直前に、所望の生理活性物質を固定して用いることができる。
(1)基板の作成
6-Amino-1-octanethiol, hydrochloride(同仁化学社製)の1mM水溶液を作成した。この溶液をA液と呼ぶ。
スパッタ装置の基板ホルダにプリズムを取り付け、真空(ベースプレッシャー1×10−3-3Pa以下)に引いてからArガスを導入し(1Pa)、基板ホルダを回転(20rpm)させながら、基板ホルダにRFパワー(0.5kW)を約9分間印加してプリズム表面をプラズマ処理(基板エする。次に、Arガスを止めて真空に引き、Arガスを再び導入し(0.5Pa)、基板ホルダを回転(10〜40rpm)させながら、8inchのCrターゲットにDCパワー(0.2kW)を約30秒間印加して2nmのCr薄膜を成膜する。次に。Arガスを止めて再び真空に引き、Arガスを再び導入し、(0.5Pa)、基板ホルダを回転(20rpm)させながら、8inchのAuターゲットにDCパワー(1kW)を約50秒間印加して50nm程度のAu薄膜を成膜した。
1重量%のCMD(名糖産業製:分子量100万)溶液20gを溶解した後、表1に記載の濃度のEDC(1-Ethyl-3-[3-Dimethylaminopropyl]carbodiimide Hydrochloride)を20ml加え、室温で1時間攪拌した。
(1)で作成された基板の上に、(2)で作成されたCMDとEDCの混合溶液を1ml滴下し、1000 rpmで45 秒スピンコートし、室温で15分間静置させて反応させることで、アミノ基を有する基板上にカルボキシメチルデキストラン薄膜を固定させた。1 N NaOH水溶液に30分浸漬し、超純水で5回洗浄することで、CMD膜を成膜したセンサースティック(サンプル1から5)を得た。各CMD膜の厚さはエリプソメーターで測定した。結果を表1に示す。
実施例1で作成したサンプル2、4及び5のCMD膜に対して2重量%のデキストラン(名糖産業製:平均分子量10万)の水溶液を0.1mlキャストし、500rpmで45秒間スピンコートし、デキストランをオーバーコートしたセンサースティック(サンプル2、4及び5)を得た。デキストラン膜の厚さは、同様にエリプソメーター測定による塗布前後の差分で算出した。結果を表1に示す。
実施例3は、実施例1及び2で得られたセンサー試料に対する保存性に関するものである。実施例1及び2で作成した試料(サンプル1から5)をアルミ袋に入れ、窒素を充填した後密閉し、50℃で2週間経時させた。これを1N NaOH水溶液に15分浸漬し、超純水で5回洗浄したものをエリプソメーターで測定し、残存しているCMD膜厚を測定した。
Dex:デキストラン
経時:50℃2週間
20 全反射プリズム(光学ブロック)
21 プリズム本体
21a 上面
22 把持部
22a 溝
23 突出部
25 金属膜(薄膜層)
26 高分子膜
27 系合爪
28 係合部
28a 系合面
29a 基準平面
30 流路部材
31 第1流路
32 第2流路
33 本体部
34 取付部
35 系合孔
36 開口
SS1 センサー面
SS2 センサー面
Claims (6)
- 金属基板の表面上に被覆した自己組織化膜の上に、第一の親水性高分子層が固定されているバイオセンサーにおいて、前記第一の親水性高分子層の上に第二の親水性高分子層が被覆されており、前記第一の親水性高分子層及び前記第二の親水性高分子層の合計の乾燥時の膜厚が20nm以上100nm以下であり、第一の親水性高分子層を形成する親水性高分子がカルボキシル基含有多糖類であり、第二の親水性高分子層を形成する親水性高分子が、解離性基を有しないノニオン性化合物であることを特徴とするバイオセンサー。
- 自己組織化膜が、メルカプト基を有する化合物により形成されたものである、請求項1に記載のバイオセンサー。
- 自己組織化膜が、下記一般式A−1で表される化合物により形成されたものである、請求項1又は2に記載のバイオセンサー。
- 自己組織化膜が、炭素数4以上10以下の直鎖アルキル基を有する化合物の水溶液を金属基板の表面上に被覆することによって形成されたものである、請求項1から3の何れかに記載のバイオセンサー。
- 非電気化学的検出に使用される請求項1から4の何れかに記載のバイオセンサー。
- 表面プラズモン共鳴分析に使用される、請求項1から請求項5の何れかに記載のバイオセンサー。
Priority Applications (5)
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JP2007248500A JP5021408B2 (ja) | 2007-09-26 | 2007-09-26 | バイオセンサー |
US12/237,498 US20100278696A1 (en) | 2007-09-26 | 2008-09-25 | Biosensor |
EP08016874A EP2042609B1 (en) | 2007-09-26 | 2008-09-25 | Biosensor |
DE602008004037T DE602008004037D1 (de) | 2007-09-26 | 2008-09-25 | Biosensor |
AT08016874T ATE492645T1 (de) | 2007-09-26 | 2008-09-25 | Biosensor |
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JP2007248500A JP5021408B2 (ja) | 2007-09-26 | 2007-09-26 | バイオセンサー |
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US (1) | US20100278696A1 (ja) |
EP (1) | EP2042609B1 (ja) |
JP (1) | JP5021408B2 (ja) |
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JPS5953836A (ja) | 1982-09-21 | 1984-03-28 | Fuji Photo Film Co Ltd | 感光性平版印刷版 |
JPS5971048A (ja) | 1982-10-18 | 1984-04-21 | Mitsubishi Chem Ind Ltd | 光重合系感光性組成物 |
SE462454B (sv) * | 1988-11-10 | 1990-06-25 | Pharmacia Ab | Maetyta foer anvaendning i biosensorer |
JPH0816664B2 (ja) * | 1989-04-18 | 1996-02-21 | 松下電器産業株式会社 | バイオセンサおよびその製造法 |
JPH06167443A (ja) | 1992-10-23 | 1994-06-14 | Olympus Optical Co Ltd | 表面プラズモン共鳴を利用した測定装置 |
JP2000039401A (ja) * | 1998-03-24 | 2000-02-08 | Dainippon Printing Co Ltd | 表面プラズモン共鳴バイオセンサ―用測定セル及びその製造方法 |
DE19818360C2 (de) * | 1998-04-24 | 2000-05-31 | Suisse Electronique Microtech | Dextranbeschichtete Oberfläche |
JP3399836B2 (ja) | 1998-05-21 | 2003-04-21 | 富士写真フイルム株式会社 | 表面プラズモンセンサー |
JP2001330560A (ja) | 2000-03-16 | 2001-11-30 | Fuji Photo Film Co Ltd | 全反射減衰を利用した測定方法および装置 |
DE10064146A1 (de) * | 2000-12-22 | 2002-07-04 | Andreas Hofmann | Biosensor und verfahren zu seiner Herstellung |
US6894200B2 (en) | 2001-01-23 | 2005-05-17 | Air Products And Chemicals, Inc. | Synthesis of vicinal difluoro aromatics and intermediates thereof |
FI118061B (fi) * | 2001-09-24 | 2007-06-15 | Beanor Oy | Menetelmä ja bioanturi analyysiä varten |
JP4312478B2 (ja) | 2003-03-12 | 2009-08-12 | 独立行政法人農業生物資源研究所 | Uvde発現による相同組換え頻度の向上 |
WO2005017122A2 (en) * | 2003-08-12 | 2005-02-24 | Arizona Board Of Regents, Acting For And On Behalf Of, Arizona State University | Biocompatibile linkers for surface plasmon resonance biosensors |
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JP4397304B2 (ja) | 2004-08-18 | 2010-01-13 | 富士フイルム株式会社 | バイオセンサー |
JP2006090781A (ja) | 2004-09-22 | 2006-04-06 | Fuji Photo Film Co Ltd | バイオセンサー |
JP2006065807A (ja) | 2004-08-30 | 2006-03-09 | Ricoh Co Ltd | 手順書作成方法及び手順書作成プログラム |
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JP4580291B2 (ja) * | 2005-06-30 | 2010-11-10 | 富士フイルム株式会社 | バイオセンサーを用いた測定方法 |
US7563623B2 (en) * | 2006-02-22 | 2009-07-21 | Fujifilm Corporation | Biosensor |
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EP2042609B1 (en) | 2010-12-22 |
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DE602008004037D1 (de) | 2011-02-03 |
US20100278696A1 (en) | 2010-11-04 |
ATE492645T1 (de) | 2011-01-15 |
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