JP4947725B2 - ジアセチルポリアミンの分解防止法 - Google Patents
ジアセチルポリアミンの分解防止法 Download PDFInfo
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- JP4947725B2 JP4947725B2 JP2008085098A JP2008085098A JP4947725B2 JP 4947725 B2 JP4947725 B2 JP 4947725B2 JP 2008085098 A JP2008085098 A JP 2008085098A JP 2008085098 A JP2008085098 A JP 2008085098A JP 4947725 B2 JP4947725 B2 JP 4947725B2
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- Prior art keywords
- diacetylpolyamine
- solution
- inhibitor
- peptidase
- proteinase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C231/00—Preparation of carboxylic acid amides
- C07C231/22—Separation; Purification; Stabilisation; Use of additives
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/17—Nitrogen containing
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
(2)ジアセチルポリアミンを含む溶液にプロテイナーゼ阻害剤及び/又はペプチダーゼ阻害剤を添加し、かつpHを5.5以上6.5以下にすることで、プロテイナーゼ及び/又はペプチダーゼによるジアセチルポリアミンの分解を防止する方法。
(3)(1)または(2)に記載の方法でジアセチルポリアミンの分解が抑制されたジアセチルポリアミン含有溶液。
(4)ジアセチルポリアミンを測定するために調製した生体由来の試料である、(3)記載の溶液。
(5)(4)記載の溶液を用いて、溶液中のジアセチルポリアミンを測定する方法。
「ジアセチルポリアミン」とは、ジアセチルスペルミン及び/又はジアセチルスペルミジンを意味し、具体的には、N1,N12−ジアセチルスペルミン、N1,N8−ジアセチルスペルミジンなどを例示することができる。
マウスの血球を洗浄後、凍結融解にて破壊したものを5%の濃度となるように生理食塩水に溶解させた。当該マウス血球破砕液に、ジアセチルスペルミンを80μMになるよう添加し、25mMトリス塩酸緩衝液を用いてpH7.4に調整した。この反応液に、プロテイナーゼ阻害剤またはペプチダーゼ阻害剤として、2mlに溶解したプロテアーゼインヒビターカクテルP2714(シグマ社)を0.2%(容量パーセント濃度、以下同様)、P8340(シグマ社)を1%になるようにそれぞれ添加した。この反応液を37℃で2時間反応させた。
実施例1と同様にして作製したマウス血球破砕液に、ジアセチルスペルミンを40μMになるように添加し、50mMリン酸緩衝液を用いてpH5.5、6.0、6.5、7.0、7.5、または8.0となるようにそれぞれpHを調整した。その後、反応液を37℃で2時間反応させた。
Claims (5)
- ジアセチルポリアミンを含む溶液にプロテイナーゼ阻害剤及び/又はペプチダーゼ阻害剤を添加するか、ジアセチルポリアミンを含む溶液のpHを5.5以上6.5以下にすることで、プロテイナーゼ及び/又はペプチダーゼによるジアセチルポリアミンの分解を防止する方法。
- ジアセチルポリアミンを含む溶液にプロテイナーゼ阻害剤及び/又はペプチダーゼ阻害剤を添加し、かつpHを5.5以上6.5以下にすることで、プロテイナーゼ及び/又はペプチダーゼによるジアセチルポリアミンの分解を防止する方法。
- 請求項1または2に記載の方法でジアセチルポリアミンの分解が抑制されたジアセチルポリアミン含有溶液。
- ジアセチルポリアミンを測定するために調製した生体由来の試料である、請求項3記載の溶液。
- 請求項4記載の溶液を用いて、溶液中のジアセチルポリアミンを測定する方法。
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2008085098A JP4947725B2 (ja) | 2008-03-28 | 2008-03-28 | ジアセチルポリアミンの分解防止法 |
US12/382,965 US20090246880A1 (en) | 2008-03-28 | 2009-03-27 | Method for preventing degradation of diacetylpolyamine |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2008085098A JP4947725B2 (ja) | 2008-03-28 | 2008-03-28 | ジアセチルポリアミンの分解防止法 |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2009236789A JP2009236789A (ja) | 2009-10-15 |
JP2009236789A5 JP2009236789A5 (ja) | 2010-10-21 |
JP4947725B2 true JP4947725B2 (ja) | 2012-06-06 |
Family
ID=41117852
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2008085098A Expired - Fee Related JP4947725B2 (ja) | 2008-03-28 | 2008-03-28 | ジアセチルポリアミンの分解防止法 |
Country Status (2)
Country | Link |
---|---|
US (1) | US20090246880A1 (ja) |
JP (1) | JP4947725B2 (ja) |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4113597A (en) * | 1976-11-26 | 1978-09-12 | Desoto, Inc. | Cathodic electrodeposition of polyamine resins using carbonic acid neutralization |
DE3833936C1 (ja) * | 1988-10-05 | 1989-09-21 | Henning Berlin Gmbh Chemie- Und Pharmawerk, 1000 Berlin, De | |
US5789261A (en) * | 1996-10-30 | 1998-08-04 | Temple University Of The Commonwealth System Of Higher Education | Solid phase immunoassay |
JP3709078B2 (ja) * | 1998-08-27 | 2005-10-19 | ヤマサ醤油株式会社 | ジアセチルポリアミンの測定法及びキット |
ATE555385T1 (de) * | 2003-03-11 | 2012-05-15 | Crc Inc | N1,n12-diacetylspermin als tumormarker |
JP4490197B2 (ja) * | 2004-07-26 | 2010-06-23 | アルフレッサファーマ株式会社 | ジアセチルスペルミンの測定方法および測定試薬キット |
JP3816512B2 (ja) * | 2005-01-24 | 2006-08-30 | 株式会社トランスジェニック | N1,n12−ジアセチルスペルミンに対する高親和性モノクローナル抗体 |
US7687231B2 (en) * | 2006-01-30 | 2010-03-30 | Yamasa Corporation | Method for determining degree of negative effect of macrophages on vertebrate |
-
2008
- 2008-03-28 JP JP2008085098A patent/JP4947725B2/ja not_active Expired - Fee Related
-
2009
- 2009-03-27 US US12/382,965 patent/US20090246880A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US20090246880A1 (en) | 2009-10-01 |
JP2009236789A (ja) | 2009-10-15 |
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