JP4803559B2 - Mushroom cultivation medium and mushroom cultivation method - Google Patents
Mushroom cultivation medium and mushroom cultivation method Download PDFInfo
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この発明は、菌床栽培に用いるキノコ栽培用培地とキノコの栽培方法に関する。 The present invention relates to a mushroom cultivation medium used for fungus bed cultivation and a mushroom cultivation method.
食用キノコ栽培において高品質なキノコを高収量で安定生産することが常に求められている。従来は培地当たりの収量の増加を図るために、培地に窒素源、ビタミン類、ミネラル、核酸関連成分等の栄養源を追加し、培地を富栄養にして解決を図っていた。 In edible mushroom cultivation, it is always required to stably produce high-quality mushrooms at a high yield. Conventionally, in order to increase the yield per medium, nutrient sources such as nitrogen sources, vitamins, minerals, and nucleic acid-related components were added to the medium, and the medium was enriched to solve the problem.
一方、培養基に含まれている栄養材を予めキノコが摂取しやすい状態に分解処理して栽培するキノコ栽培方法が特許許文献１に開示されている。このキノコ栽培方法及びキノコ栽培基は、おがくずと、米糠と、水分調整用の水と、アミラーゼとを含むキノコ培養基用混合物を、およそ９０℃以下の温度で加熱してデンプンをアルファ化してより速やかに酵素分解を受けることができるようにしてから、一定時間放置して酵素分解工程に付したのち、殺菌処理工程を施したものを、キノコ栽培基として用いるものである。これは、添加したアミラーゼにより、栄養材（米糠）等中のデンプンを完全に酵素分解してグルコースにして菌糸体の生育に良好となるものである。
上記従来の技術の、培地を富栄養とする方法では、キノコ以外の微生物にとっても栄養源が豊富で好適な培地になるため、容易に害菌汚染を招いた。さらに栄養過多の培地で栽培すると、キノコ菌糸体の生理障害を誘発して子実体の不良発生、異常発生、二次的害菌汚染を招いた。 In the above-described conventional method for enriching a culture medium, a nutrient medium is abundant and suitable for microorganisms other than mushrooms. Furthermore, when cultivated in an over-nutrition medium, it caused physiological damage to the mushroom mycelium, resulting in fruit body defects, abnormalities, and secondary contamination with bacteria.
また上記特許文献１の場合、栄養材のデンプンをアミラーゼで完全に分解するものであり、グルコースの含有量を高めることができる。この場合、グルコースの増加により多少菌糸体の生育が良くなることは予想されるが、グルコースの増加のみでは著しい効果は期待できなかった。しかも、グルコースに分解するために、およそ９０℃以下の温度で加熱してデンプンをアルファ化させるが、高温に木質成分を長くさらすと酢酸などのキノコにとって阻害成分となる有機酸が生じやすくなる。そして、栽培工程が追加され、しかも熱源のコストが高くなる問題が生じる。 Moreover, in the case of the said patent document 1, the starch of a nutrient is decomposed | disassembled completely with amylase, and content of glucose can be raised. In this case, it is expected that the growth of mycelium is somewhat improved by the increase in glucose, but a significant effect cannot be expected only by an increase in glucose. Moreover, in order to break down into glucose, the starch is pregelatinized by heating at a temperature of about 90 ° C. or less. However, when the wood component is exposed to a high temperature for a long time, an organic acid that becomes an inhibitory component for mushrooms such as acetic acid is likely to be generated. And the cultivation process is added and the problem that the cost of a heat source becomes high arises.
この発明は、上記背景技術の問題点に鑑みてなされたものであり、簡易な栽培工程で、菌床培地の栄養をキノコ菌糸体に好適な状態に変換することにより、高品質なキノコを高収量で安定生産することができるキノコ栽培用培地とキノコの栽培方法を提供することを目的とする。 The present invention has been made in view of the problems of the background art described above, and converts high-quality mushrooms to high quality by converting the nutrients of the fungus bed medium into a state suitable for mushroom mycelium in a simple cultivation process. An object of the present invention is to provide a medium for mushroom cultivation and a method for cultivating mushrooms that can be stably produced in a yield.
本発明は、培地基材と、栄養材を混合して水分調整して得られる混合物に、アミラーゼとグルコシダーゼを添加して室温で栄養材の酵素分解を行い、栄養材の多糖が分解され、単糖になる途中の低分子量の物質であるオリゴマーが生成されたキノコ栽培用培地である。 In the present invention, amylase and glucosidase are added to a mixture obtained by mixing a culture medium base material and a nutrient material to adjust the water content, and the nutrient material is enzymatically decomposed at room temperature to decompose the polysaccharide of the nutrient material. It is a mushroom cultivation medium in which an oligomer which is a low molecular weight substance in the middle of becoming a sugar is produced.
前記オリゴマーは、低分子αグルカン、及び低分子βグルカンである。 Before Kio oligomer is a low molecular α-glucan, and low molecular β glucan.
また本発明は、培地基材と、栄養材を混合して水分調整し、前記水分調整された混合物にアミラーゼとグルコシダーゼを添加し、室温で栄養材の多糖を分解し、単糖になる途中の低分子量の物質であるオリゴマーが生成されたキノコ栽培用培地で栽培するキノコの栽培方法である。 In addition, the present invention mixes a medium base material and a nutrient material to adjust moisture, adds amylase and glucosidase to the moisture-adjusted mixture, decomposes the polysaccharide of the nutrient material at room temperature, and becomes a monosaccharide This is a method for cultivating mushrooms cultivated in a mushroom cultivation medium in which an oligomer which is a low molecular weight substance is produced.
本発明のキノコ栽培用培地とキノコの栽培方法は、菌床培地の栄養をキノコ菌糸体に好適な状態に変換し、高品質なキノコを高収量で安定生産することができる。しかも、菌床培地を特別な処理を行う必要がなく、通常の栽培工程により栽培することができ、栽培も容易である。 The medium for cultivating mushrooms and the method for cultivating mushrooms according to the present invention can stably convert high-quality mushrooms in a high yield by converting the nutrients of the fungus bed medium into a state suitable for mushroom mycelium. Moreover, it is not necessary to specially treat the bacterial bed medium, and it can be cultivated by a normal cultivation process, and cultivation is easy.
以下、この発明の一実施形態について説明する。この実施形態は、培地基材と栄養材を混合して水分調整した基本培地に、多糖分解酵素を重量比で５０〜１０００ｐｐｍ混合して調整するキノコ栽培用培地である。そしてこの培地を用いて栽培するキノコの栽培方法である。多糖分解酵素は、アミラーゼとグルコシダーゼである。そして、このようなキノコ栽培用培地を用いるキノコの栽培方法である。なお、アミラーゼは耐熱性アミラーゼでも中温性アミラーゼ（室温で反応）でもよい。 Hereinafter, an embodiment of the present invention will be described. This embodiment is a mushroom cultivation medium in which a polysaccharide-degrading enzyme is mixed and adjusted in a weight ratio of 50 to 1000 ppm in a basic medium in which a medium base material and a nutrient material are mixed to adjust water content. And it is the cultivation method of the mushroom grown using this culture medium. The polysaccharide degrading enzymes are amylase and glucosidase. And it is a cultivation method of a mushroom using such a culture medium for mushroom cultivation. The amylase may be a thermostable amylase or a mesophilic amylase (reacted at room temperature).
この実施形態のキノコの栽培方法について説明する。オガコ、木材チップ等の培地基材と、米糠、フスマ、コーンブラン等の栄養材を混合し、水道水を添加して含水率を調整し基本培地とする。基本培地に所定濃度となるように多糖分解酵素を添加し、栽培用のビンなどに移し、必要に応じて殺菌処理を施し、キノコの種菌を接種し所定条件下で栽培する。 The cultivation method of the mushroom of this embodiment is demonstrated. A medium substrate such as sawdust and wood chips and a nutrient such as rice bran, bran and corn bran are mixed, and tap water is added to adjust the water content to obtain a basic medium. A polysaccharide-degrading enzyme is added to the basic medium to a predetermined concentration, transferred to a cultivation bottle or the like, sterilized as necessary, inoculated with mushroom inoculum, and cultivated under predetermined conditions.
次に、この実施形態のキノコ栽培用培地について説明する。このキノコ栽培用培地は、培地調整時に添加した多糖分解酵素との酵素反応により、栄養材のデンプンを低分子グルカンや非結晶性のグルカンに分解して、キノコ菌糸体に吸収し易い培地に変換したものである。これは、新たに栄養源を追加することなく、既存の栄養材と添加酵素が培地調整（培地の撹拌、ビンへの詰め込み）、殺菌の工程時間内で酵素反応が促されて栄養材を変換させたものである。 Next, the mushroom cultivation medium of this embodiment will be described. The mushroom cultivation medium, by enzymatic reaction with polysaccharide degrading enzyme were added at the time of the medium adjusted, starch nutritional material is decomposed into low-molecular-glucan and amorphous glucans, converted into liable medium absorbs mushroom mycelium It is a thing. This means that, without adding a new nutrient source, the existing nutrients and added enzymes can be converted into nutrients by stimulating the enzyme reaction within the medium preparation (stirring the medium, filling the bottle) and sterilization. It has been made.
殺菌工程では、室温で加熱以外の殺菌工程を用いるか、温度を低くして加熱するなどして、完全にモノマーに分解しないように設定する。これにより、デンプンをアミラーゼとグルコシダーゼで完全に分解せず、その中間段階の中間物質であって低分子量の物質であるオリゴマーが生成される。この中間物質のオリゴマーは、低分子αグルカン、低分子βグルカンである。さらに、前記中間物質は、高分子αグルカン、及び非結晶性の高分子βグルカンを含んでいる。アミラーゼにより低分子αグルカンと高分子αグルカンが生成され、βグルコシダーゼにより低分子βグルカンと非結晶性の高分子βグルカンが生成され、２種類の多糖分解酵素による相乗効果がある。特に、米糠には植物細胞壁由来のセルロースも含まれており、セルロースとデンプンが絡み合って存在していると考えられ、βグルコシダーゼを添加することによりオリゴマーのβグルカン、αグルカンが生じ易くなる。 In the sterilization process, a sterilization process other than heating is used at room temperature, or the temperature is lowered and heated so that the monomer is not completely decomposed. Thereby, the starch is not completely decomposed by amylase and glucosidase, and an oligomer that is an intermediate substance in the intermediate stage and a substance having a low molecular weight is generated. The oligomer of this intermediate substance is a low-molecular α-glucan or a low- molecular β-glucan . Moreover, the intermediate material, that contains a polymeric α-glucan, and noncrystalline polymers β-glucan. Low molecular α-glucan and high molecular α-glucan is produced by amylase, low molecular β-glucans and non-crystalline polymer β-glucan is produced by β-glucosidase, a synergistic effect of the two types of polysaccharide degrading enzymes. In particular, rice bran contains cellulose derived from plant cell walls, and it is considered that cellulose and starch are entangled with each other. By adding β-glucosidase, oligomeric β-glucan and α-glucan are easily generated.
この実施形態のキノコ栽培用培地とキノコの栽培用法によれば、菌床培地の栄養をキノコ菌糸体に好適な状態に変換することにより高品質なキノコを高収量で安定生産することができる。子実体収量が増加して培地当たりの収量も増加して生産性の向上が図られ、また子実体の大形化が可能であり多産地との差別化を図ることができる。シイタケ、エリンギの市場では大きな子実体は高値で取引されており、最近ではナメコでも大きな子実体は大粒ナメコとして高く取引されていることから、子実体の大形化により収益性の向上に寄与するものである。なお具体的には、培地調整時にアミラーゼ、グルコシダーゼを５０〜１０００ｐｐｍ添加することでナメコでは子実体収量が２割増加し、子実体個体重が２〜４割増加した。またヤマブシタケではアミラーゼ、グルコシダーゼを２５０〜１０００ｐｐｍ添加することにより子実体収量が５〜６割増加した。 According to the mushroom cultivation medium and the mushroom cultivation method of this embodiment, high-quality mushrooms can be stably produced at a high yield by converting the nutrients of the fungus bed medium to a state suitable for mushroom mycelium. The fruiting body yield is increased and the yield per medium is increased to improve the productivity, and the fruiting body can be enlarged and differentiated from the production area. Large fruit bodies are traded at high prices in the Shiitake and Elingi markets, and recently, large fruit bodies are also traded as large-sized sea cucumbers at high prices, and this contributes to improving profitability by increasing the size of fruit bodies. Is. More specifically, by adding 50 to 1000 ppm of amylase and glucosidase when adjusting the medium, the fruit body yield increased by 20% and the body weight of the fruit body increased by 20 to 40%. In Yamabushitake, adding amylase and glucosidase in an amount of 250 to 1000 ppm increased the fruiting body yield by 50 to 60%.
この実施形態のキノコ栽培用培地とキノコの栽培用法は、酵素を添加するだけでよく、酵素反応の工程を設定しなくても、室温で、通常の培地調整、殺菌工程の時間内で酵素反応を促すことができ、容易に既存の栄養材を低分子オリゴマーに変換し、新たに栄養源を追加することなく、子実体収量の増加を図ることができる。工程も簡潔であることから、現場に容易に適応する栽培方法である。生産規模を問わずに幅広く利用することができ、現在キノコ栽培に取り組んでいる生産者が利用することができる。酵素反応を促す加熱工程がないため、高温に木質成分を長くさらすと生じる酢酸等のキノコの阻害成分である有機酸が生じることがなく、安全である。 The mushroom cultivation medium and the mushroom cultivation method of this embodiment need only add enzyme, and without setting the enzyme reaction process, at room temperature, the enzyme reaction within the normal medium preparation and sterilization process time Can easily convert existing nutrients into low-molecular oligomers and increase fruit body yield without adding a new nutrient source. Since the process is also simple, it is a cultivation method that is easily adapted to the field. It can be used widely regardless of production scale, and can be used by producers currently working on mushroom cultivation. Since there is no heating step that promotes an enzymatic reaction, organic acids that are mushroom-inhibiting components such as acetic acid, which are generated when a woody component is exposed to high temperatures for a long time, are safe and safe.
また、新たに栄養源を追加しないため、害菌汚染のリスクは低く、キノコの生理障害が誘発されにくいものである。そして、既存の栄養を低分子化するだけなので安全であり、消費者が安心できる安全なキノコを提供することができる。アミラーゼとグルコシダーゼは食品添加物に利用されている酵素を利用することから、安心、安全なキノコの安定供給を求めている消費者ニーズと一致し消費の拡大が見込まれる。 In addition, since no new nutrient source is added, the risk of contamination with harmful fungi is low, and physiological disorders of mushrooms are difficult to induce. And it is safe because it only reduces the molecular weight of existing nutrition, and can provide safe mushrooms that consumers can feel secure. Since amylase and glucosidase use enzymes used for food additives, consumption is expected to increase in line with consumer needs for a stable supply of safe and safe mushrooms.
また、酵素製造メーカは酵素の新規用途を開拓することができる。キノコ生産者と酵素製造メーカのニーズに適っていることから、高い市場性を期待することができる。 Enzyme manufacturers can also explore new uses for enzymes. Because it meets the needs of mushroom producers and enzyme manufacturers, high marketability can be expected.
なお、この発明のキノコ栽培用培地とキノコの栽培方法は、上記実施形態に限定されず、アミラーゼまたはグルコシダーゼを単独で使用してもよく、他の多糖分解酵素を混合して使用してもよい。多糖分解酵素を添加する基本培地の材料は適宜設定可能であり、いろいろな種類のキノコの栽培に使用することができる。 The mushroom cultivation medium and the mushroom cultivation method of the present invention are not limited to the above embodiment, and amylase or glucosidase may be used alone, or other polysaccharide-degrading enzymes may be mixed and used. . The material of the basic medium to which the polysaccharide degrading enzyme is added can be set as appropriate, and can be used for the cultivation of various types of mushrooms.
次に、ナメコの栽培について具体的な実施例を挙げて本発明を説明する。まず、培地基材にブナオガコを用い、栄養材にフスマ・コーンブラン（１：１重量比）を用いた。基本培地（対照区）は、培地基材と栄養材を３：１（重量比）で混合し、水道水を添加して含水率を６３％（湿量基準）に調整した。 Next, the present invention will be described with specific examples of nameko cultivation. First, beech was used as a medium substrate, and bran corn bran (1: 1 weight ratio) was used as a nutrient. In the basic medium (control group), the medium base material and the nutrient material were mixed at a ratio of 3: 1 (weight ratio), and tap water was added to adjust the water content to 63% (wet basis).
多糖分解酵素としてアミラーゼとグルコシダーゼを供試した。アミラーゼは市販酵素（ＮＯＶＯ社製）のＢＡＮ（中温性アミラーゼ）ならびＴｅｒｍａｍｙｌ（耐熱性アミラーゼ）を使用した。グルコシダーゼはｅｎｄｏ−１，３，β−Ｄ−グルコシダーゼ（Ｍｅｇａｚｙｍｅ社製）を用いた。 Amylase and glucosidase were used as polysaccharide degrading enzymes. As the amylase, BAN (medium-temperature amylase) and Thermalmyl (thermostable amylase), which are commercially available enzymes (manufactured by NOVO), were used. As the glucosidase, endo-1,3, β-D-glucosidase (manufactured by Megazyme) was used.
多糖分解酵素の、基本培地への添加量は、ＢＡＮ、Ｔｅｒｍａｍｙｌでは、全培地重量に対して１０，２５，５０，１００，２５０，５００，１０００ｐｐｍ添加した。ｅｎｄｏ−１，３，β−Ｄ−グルコシダーゼは、５０，１００，２５０，５００，１０００ｐｐｍ添加した。また、アミラーゼとグルコシダーゼの混合培地（以下、ＴＧ混合培地とする。）として、Ｔｅｒｍａｍｙｌとｅｎｄｏ−１，３，β−Ｄ−グルコシダーゼをそれぞれ５０，１００，２５０，５００，１０００ｐｐｍ添加した。 The amount of polysaccharide degrading enzyme added to the basic medium was 10, 25, 50, 100, 250, 500, 1000 ppm with respect to the total medium weight in BAN and Termamyl. Endo-1,3, β-D-glucosidase was added at 50, 100, 250, 500, 1000 ppm. Further, as a mixed medium of amylase and glucosidase (hereinafter referred to as TG mixed medium), Thermalmyl and endo-1,3, β-D-glucosidase were added at 50, 100, 250, 500, and 1000 ppm, respectively.
上記の多糖分解酵素を添加したキノコ栽培用培地を、８００ｍｌポリプロピレン製ビンに５００ｇ詰め、供試菌としてナメコ市販菌キノックスＮ００２号を接種し、２２±２℃で６０日間培養後、１５±２℃、ＲＨ９０％の条件で子実体形成を促した。 The above-mentioned polysaccharide-degrading enzyme-added mushroom cultivation medium was packed in 500 ml in 800 ml polypropylene bottles, inoculated with nameko commercially available fungus Kinox N002, and cultured at 22 ± 2 ° C. for 60 days, then 15 ± 2 ° C. , Promoted the formation of fruiting bodies under the condition of RH 90%.
この栽培試験の結果を表１に示す。
この結果、この実施例では子実体収量に関して、アミラーゼ、グルコシダーゼを添加することで、基本培地（対照区）での栽培に対して、子実体収量は増加した。ＢＡＮ添加培地では５０〜５００ｐｐｍの添加で１〜２割、Ｔｅｒｍａｍｙｌ添加培地では５０〜１０００ｐｐｍ添加培地で１〜２割、子実体収量が増加した。ｅｎｄｏ−１，３，β−Ｄ−グルコシダーゼでは、５００ｐｐｍ添加のみで有意に増加し、他の添加濃度では優位差がなかった。このことからグルコシダーゼに比べアミラーゼで有効に作用したことがわかった。両者を混合したＴＧ培地では、５０〜１０００ｐｐｍ添加培地で、対照区に対して２割前後増加し、Ｔｅｒｍａｍｙｌ単独で添加したした場合より子実体収量が多くなる傾向を示し、相乗効果が認められた。 As a result, in this Example, the fruiting body yield increased with respect to the cultivation in the basic medium (control group) by adding amylase and glucosidase. In the BAN-added medium, the yield of fruiting bodies was increased by 10 to 20% with the addition of 50 to 500 ppm, and with the Termamyl added medium, 10 to 20% with the medium with 50 to 1000 ppm added. In endo-1,3, β-D-glucosidase, it increased significantly only by addition of 500 ppm, and there was no superior difference at other addition concentrations. From this, it was found that amylase acted more effectively than glucosidase. In the TG medium mixed with both, the medium with 50 to 1000 ppm increased about 20% compared to the control group, and the fruit body yield tended to increase more than when added with Termamyl alone, and a synergistic effect was observed. .
子実体発生個数は、ＢＡＮ添加培地の２５ｐｐｍ，５００ｐｐｍ、Ｔｅｒｍａｍｙｌ添加培地の５００ｐｐｍ、１０００ｐｐｍで対照区に比べ発生個数が少なくなったが、他の試験区では対照区と有意差がなかったことから、発生個数には大きな影響はなかったと考えられる。子実体発生個数に影響がないか、やや少なくなる傾向を示したので、子実体収量が増加した試験区では、子実体個体重が大きくなる傾向を示した。 The number of fruiting bodies was 25 ppm and 500 ppm in the BAN-added medium, 500 ppm and 1000 ppm in the Termamyl-added medium, and the number of occurrences was smaller than in the control group, but there was no significant difference from the control group in the other test groups. It seems that there was no significant effect on the number of occurrences. Since there was no effect on the number of fruiting bodies or a tendency to decrease slightly, the body weight of the fruiting bodies showed a tendency to increase in the test plot where the fruiting body yield increased.
これらのことからアミラーゼ、グルコシダーゼを５０〜１０００ｐｐｍ添加することでナメコでは子実体収量が２割増加し、子実体固体重が２〜４割増加した。 From these facts, the addition of 50 to 1000 ppm of amylase and glucosidase increased the fruit body yield by 20% and the fruit body solid weight by 20 to 40%.
また、栽培所要日数に関して、対照区が８０．３日間に対してｅｎｄｏ−１，３，β−Ｄ−グルコシダーゼ添加培地では対照区と優位差がなく同程度の栽培所要日数であったが、他の酵素添加培地では栽培所要日数は長くなる傾向をしめした。ＢＡＮでは、５０〜１００ｐｐｍの添加で２〜３日間、Ｔｅｒｍａｍｙｌでは５０〜１０００ｐｐｍで１．５〜２．５日間、ＴＧ混合培地では５０〜１０００ｐｐｍの添加で１〜２日間、有意に長くなった。 In addition, regarding the number of days required for cultivation, the control group had the same number of days required for cultivation in the endo-1,3, β-D-glucosidase-added medium as compared to the control group for 80.3 days. In the enzyme-supplemented medium, the number of days required for cultivation tended to be longer. In BAN, the addition of 50 to 100 ppm was prolonged for 2 to 3 days, in Termamyl, 50 to 1000 ppm at 1.5 to 2.5 days, and in the TG mixed medium, the addition of 50 to 1000 ppm was significantly prolonged for 1 to 2 days.
次に、ヤマブシタケの栽培について具体的な実施例を挙げて本発明を説明する。まず、培地基材にブナオガコを用い、栄養材にフスマを用いた。基本培地（対照区）は、培地基材と栄養材を１：１（重量比）で混合し、水道水を添加して含水率を６３％（湿量基準）に調整した。 Next, the present invention will be described with reference to specific examples of cultivation of Yamabushitake. First, beech was used as a medium substrate and bran was used as a nutrient. In the basic medium (control group), the medium base material and the nutrient material were mixed at 1: 1 (weight ratio), and tap water was added to adjust the water content to 63% (humidity standard).
供試酵素は、上記実施例１と同様である。ＢＡＮ、Ｔｅｒｍａｍｙｌの添加量は、全培地重量に対して５０，１００，２５０，５００，１０００ｐｐｍ添加した。ｅｎｄｏ−１，３，β−Ｄ−グルコシダーゼは、実施例１と同様に５０，１００，２５０，５００，１０００ｐｐｍ添加した。アミラーゼとグルコシダーゼの混合培地であるＴＧ混合培地は、実施例１と同様に、Ｔｅｒｍａｍｙｌとｅｎｄｏ−１，３，β−Ｄ−グルコシダーゼをそれぞれ５０，１００，２５０，５００，１０００ｐｐｍ添加した。 The test enzyme is the same as in Example 1 above. BAN and Termamyl were added at 50, 100, 250, 500, and 1000 ppm with respect to the total medium weight. Endo-1,3, β-D-glucosidase was added in the same manner as in Example 1 at 50,100,250,500,1000 ppm. As in Example 1, TG mixed medium, which is a mixed medium of amylase and glucosidase, was added with Termamyl and endo-1,3, β-D-glucosidase at 50, 100, 250, 500, and 1000 ppm, respectively.
上記の多糖分解酵素を添加した培地を実施例１と同様の工程で処理し、ヤマブシタケの種菌を接種し、同様の条件で子実体形成を促した。 The medium supplemented with the above polysaccharide-degrading enzyme was treated in the same manner as in Example 1 and inoculated with inoculum of Yamabushitake to promote fruiting body formation under the same conditions.
この栽培試験の結果を表２に示す。
この結果、この実施例でも子実体収量に関して、アミラーゼ、グルコシダーゼを５０〜１０００ｐｐｍ添加することで、基本培地（対照区）での栽培に対して、子実体収量は増加した。ＢＡＮ添加培地では対照区に対して２〜３割、Ｔｅｒｍａｍｙｌ添加培地では１．５〜４割、ｅｎｄｏ−１，３，β−Ｄ−グルコシダーゼでは２〜５割増加した。ＢＡＮ、Ｔｅｒｍａｍｙｌ、ｅｎｄｏ−１，３，β−Ｄ−グルコシダーゼの最適な添加量は、それぞれ１００〜５００ｐｐｍ、１００〜１０００ｐｐｍ、２５０〜１０００ｐｐｍとなった。ＴＧ混合培地では、単独で分解酵素を添加した場合より子実体収量は多くなり、それぞれを２５０〜１０００ｐｐｍ添加すると子実体収量は５〜６割増加し、各酵素の相乗効果が認められた。 As a result, also in this Example, regarding the fruit body yield, by adding 50 to 1000 ppm of amylase and glucosidase, the fruit body yield increased compared to the cultivation in the basic medium (control group). In the BAN-added medium, it increased by 20 to 30% with respect to the control group, with 1.5 to 40% in the Termamyl-added medium, and 20 to 50% in the endo-1,3, β-D-glucosidase. The optimum addition amounts of BAN, Thermalyl, endo-1,3, β-D-glucosidase were 100 to 500 ppm, 100 to 1000 ppm, and 250 to 1000 ppm, respectively. In the TG mixed medium, the fruiting body yield increased compared to the case where the degrading enzyme was added alone, and when each of 250 to 1000 ppm was added, the fruiting body yield increased by 50 to 60%, and the synergistic effect of each enzyme was recognized.
このことからアミラーゼ、グルコシダーゼを２５０〜１０００ｐｐｍ添加することにより子実体収量が５〜６割増加した。
From this, the fruiting body yield increased by 50-60% by adding 250-1000 ppm of amylase and glucosidase.
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