JP4779433B2 - ろ過フィルタデバイス - Google Patents
ろ過フィルタデバイス Download PDFInfo
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- JP4779433B2 JP4779433B2 JP2005142652A JP2005142652A JP4779433B2 JP 4779433 B2 JP4779433 B2 JP 4779433B2 JP 2005142652 A JP2005142652 A JP 2005142652A JP 2005142652 A JP2005142652 A JP 2005142652A JP 4779433 B2 JP4779433 B2 JP 4779433B2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
図1に合成高分子製多孔質ろ過膜1に外縁構造体2を備える形態を示す。外縁構造体の構成の違いにより3種類の形態を示す。図1(a)は外縁構造体2 を二枚のシート状材で、好ましくは吸水性を有しない合成樹脂材で構成し合成高分子製多孔質ろ過膜1を挟むように構成したもの、図1(b)は外縁構造体2を1枚のシート状材で、好ましくは吸水性を有しない合成樹脂材で構成し合成高分子製多孔質ろ過膜1を外縁構造体2に溶着させて構成したもの、図1(c)は外縁構造体2を二個の樹脂成形材で構成し合成高分子製多孔質ろ過膜1を挟み込むように構成したものである。これらのろ過フィルタデバイスにはろ過開口3を備える。また検体溶液をろ過するには、図2に示すろ過器を用いればよい。多孔性台4の下にベース5が配置され、多孔性台4は外縁構造体の厚みを避け、合成高分子製多孔質ろ過膜1が多孔性台4に密着できるように凸形状を備えている点が前述のろ過器とは異なる。ベース5にろ過フィルタデバイスを置く、あるいはベース5よりはずすときには、ろ過フィルタデバイスの外縁構造体2をつまんで行えばよく、膜を汚染させたり、傷つけたりすることなく取扱いが容易となる。
ろ過が終わったろ過フィルタデバイスは顕微鏡にて観察をする。図3と図4に示すように、顕微鏡ステージ部分に平滑基準台6が備えておく。平滑基準台は上面の平滑度が好ましくは2μmほどに仕上げた錆びにくい硬質体で、好ましくはステンレス材、セラミックス材などで構成されている。図4(a)はろ過フィルタデバイスの断面図であり、図4(b)は同A部詳細断面図である。
図5と図6にろ過フィルタデバイスの合成高分子製多孔質ろ過膜に対して外周方向に張力を加える別な構成を示す。図6(a)はろ過フィルタデバイスの断面図であり、図6(b)は同B部詳細断面図である。図5に示すように基準面7には凸突起a10が備えてある。凸突起a10の高さ0.3mm、根元巾0.5mmとする。また、図6の断面図で示すように外縁構造体2ろ過上流側にあたる部品において合成高分子製多孔質ろ過膜1に接する面に基準面7の凸突起a10と位置、直径が一致する深さ0.5mm、溝巾1mmの凹溝a11を備える。B部詳細で示すようにこの凸突起a10と凹溝a11とで合成高分子製多孔質ろ過膜1を全周で挟むことで外周方向に張力を加わえることができる。これにより合成高分子製多孔質ろ過膜1は平滑基準台6凸面基準面に合わされる。また前述例同様、合成高分子製多孔質ろ過膜1を外縁構造体2に組付け、ろ過フィルタデバイスとした場合に合成高分子製多孔質ろ過膜1に若干のうねり、しわが残っていても平滑基準台6凸面基準面に合わされ平面化するという作用もある。
図8(a)は外縁構造体2ろ過開口a16より小さい内径をもち、ろ過内開口a16が丸形状の第2の縁部材15を備えた姿を示す。
図10は、DNAプローブ109を合成高分子製多孔質ろ過膜1の孔以外の部分に固定化したものを示す。DNAを含んだ検体は、ろ過開口a16に注がれ、合成高分子製多孔質ろ過膜1に固定してあるDNAプローブ109と反応し結合する。結合後、図には示していないが、合成高分子多孔質ろ過膜1の下に設けた多孔性台4にDNAプローブ109と反応しない液体が吸引除去され、反応したDNAのみを合成高分子多孔質ろ過膜1上に残存することができる。残存したDNAは事前に染色もしくは蛍光基や発光基が標識されており、標識を検出することで、反応部が容易に検出することができる。
合成高分子製多孔質ろ過膜表面での焦点とほぼ同じ位置となり、容易に観察することができる。さらに、孔の壁面に反応するDNAプローブを固定化することで、検体接触を確実に行える構成になることは、言うまでもない。
図11に格子状の孔を持つ格子状多孔質ろ過膜17と顕微鏡観察時のXYステージを利用する概要を示すが、観察者はXYステージを移動させると同時に格子状多孔質ろ過膜17の孔の格子に従うように観察することができ、格子の辺の位置を明確にするため、 格子位置決めマーカ18を外縁構造体2に設置してある。格子位置決めマーカ18によりXYステージ移動時、X軸に対し0℃、90℃の位置に合わせることでXY移動とともに格子状多孔質ろ過膜17が移動し、格子状多孔質ろ過膜17の孔内で反応した現象を容易に観察できる。図12には、格子状多孔質ろ過膜17の詳細を示す。格子部分の孔内部にDNAプローブ109を固定化することで、焦点の合わせは勿論容易になる。
2 外縁構造体
3 ろ過開口
4 多孔性台
5 ベース
6 平滑基準台
7 基準面
8 デバイス押え機構
9 排水排気孔
10 凸突起a
11 凹溝a
12 凸突起b
13 凹溝b
14 凹溝c
15 第2の縁部材
16 ろ過開口a
17 格子状多孔質ろ過膜
Claims (3)
- 多孔質ろ過膜の内側にろ過開口を備えた外縁構造体を設け、前記外縁構造体の前記ろ過開口より小さい内径をもち、ろ過内開口が丸形状の第2の縁部材を備え、顕微鏡観察時は第2の縁部材を取り外して行うことを特徴とするろ過フィルタデバイス。
- 第2の縁部材のろ過内開口が、四角開口、複数開口と丸開口以外の形状とすることを特徴とする請求項1記載のろ過フィルタデバイス。
- 多孔質ろ過膜を合成高分子製としたことを特長とする請求項1または2に記載のろ過フィルタデバイス。
Priority Applications (1)
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JP2005142652A JP4779433B2 (ja) | 2004-08-26 | 2005-05-16 | ろ過フィルタデバイス |
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JP2004246696 | 2004-08-26 | ||
JP2004246696 | 2004-08-26 | ||
JP2005142652A JP4779433B2 (ja) | 2004-08-26 | 2005-05-16 | ろ過フィルタデバイス |
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JP2006087424A JP2006087424A (ja) | 2006-04-06 |
JP4779433B2 true JP4779433B2 (ja) | 2011-09-28 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11369350B2 (en) | 2012-11-20 | 2022-06-28 | The Trustees Of Columbia University In The City Of New York | Medical apparatus and method for collecting biological samples |
Families Citing this family (16)
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---|---|---|---|---|
US20080113357A1 (en) * | 2006-06-29 | 2008-05-15 | Millipore Corporation | Filter device for the isolation of a nucleic acid |
JP2008051803A (ja) * | 2006-07-28 | 2008-03-06 | Sharp Corp | 分析用マイクロ流路デバイス |
KR20090119838A (ko) * | 2007-02-16 | 2009-11-20 | 엔테그리스, 아이엔씨. | 고분자 멤브레인과 금속 지지구들을 갖는 유체 필터 |
EP2183383B1 (en) * | 2007-08-01 | 2013-01-16 | Hitachi Chemical Co., Ltd. | Pathogen detection in large-volume particulate samples |
FR2945457B1 (fr) * | 2009-05-12 | 2011-07-15 | Aes Chemunex | Ensemble forme d'une membrane de filtration et d'une plaque de support, ainsi que dispositif de test microbiologique en faisant usage. |
US9448163B2 (en) | 2010-03-05 | 2016-09-20 | Konica Minolta Holdings, Inc. | Method for detecting rare cell on observation region |
JP6482459B2 (ja) | 2012-05-02 | 2019-03-13 | チャールズ リバー ラボラトリーズ, インコーポレイテッド | 細胞サンプル中の生存細胞を検出する方法 |
WO2013166338A2 (en) * | 2012-05-02 | 2013-11-07 | Charles River Laboratories, Inc. | Cell capture system and use thereof |
WO2015066721A1 (en) * | 2013-11-04 | 2015-05-07 | Charles River Laboratories, Inc. | Filtration system and use thereof |
JP2015188314A (ja) * | 2014-03-27 | 2015-11-02 | 日立化成株式会社 | 細胞捕捉金属フィルタ、細胞捕捉金属フィルタシート、細胞捕捉デバイス、細胞捕捉金属フィルタの製造方法、及び、細胞捕捉金属フィルタシートの製造方法 |
JP6476573B2 (ja) * | 2014-03-27 | 2019-03-06 | 日立化成株式会社 | 細胞捕捉金属フィルタシート、細胞捕捉金属フィルタ、細胞捕捉デバイス、及び、細胞捕捉金属フィルタシートの製造方法 |
JP2018143898A (ja) * | 2015-07-31 | 2018-09-20 | 株式会社村田製作所 | 濾過フィルタデバイス |
CN106289906B (zh) * | 2016-08-08 | 2024-02-23 | 湖南省天骑医学新技术股份有限公司 | 一种微孔滤膜制片装置及其在制片和显微镜观察时的装配方法 |
CN106635747A (zh) * | 2017-02-15 | 2017-05-10 | 中国人民解放军军事医学科学院基础医学研究所 | 一种纸基微流控快速核酸提取装置 |
CN108760458B (zh) * | 2018-07-25 | 2024-01-30 | 湖南省天骑医学新技术股份有限公司 | 一种快速固定微孔滤膜的方法及设备 |
JP2021096139A (ja) * | 2019-12-17 | 2021-06-24 | 日本バイリーン株式会社 | 細胞観察標本作製用細胞保持基材 |
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JPH0752120Y2 (ja) * | 1989-10-18 | 1995-11-29 | ローレルバンクマシン株式会社 | ベルト搬送装置におけるプーリの固定構造 |
JP3531196B2 (ja) * | 1994-01-25 | 2004-05-24 | 東洋紡績株式会社 | 多孔性膜上の発光反応による物質の検出法およびその装置 |
JP2000046702A (ja) * | 1998-07-31 | 2000-02-18 | Matsushita Electric Ind Co Ltd | 試料捕集用フィルタ |
JP3907508B2 (ja) * | 2001-07-30 | 2007-04-18 | 松下エコシステムズ株式会社 | 微生物採取チップ、微生物採取キット、微生物計量方法及び微生物計量装置 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11369350B2 (en) | 2012-11-20 | 2022-06-28 | The Trustees Of Columbia University In The City Of New York | Medical apparatus and method for collecting biological samples |
US11918192B2 (en) | 2012-11-20 | 2024-03-05 | The Trustees Of Columbia University In The City Of New York | Medical apparatus and method for collecting biological samples |
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JP2006087424A (ja) | 2006-04-06 |
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