JP4688667B2 - 標本画像化用走査型イメージャ及び画像化方法 - Google Patents
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
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Description
標本に対する輻射ビームの作用によって生じ、入射側開口にて受光され、光路を介して出射側開口に伝送され、出射側開口から出射される光信号を各輻射源に対応づけて受光検知する光検知器システムと、光検知器システムにより検知された光信号を各輻射源に対応づけて処理するプロセッサと、を備える標本画像化用走査型イメージャであって、各輻射源は、各輻射源に対応する回転式ポリゴンスキャナの各反射面によって反射される輻射ビームを発し、反射された各輻射ビームが各輻射ビームの走査方向と直角方向にずれた標本の位置を各輻射ビームの標本に対する作用時間をずらして順番に走査することができ、回転式ポリゴンスキャナの一回転あたりの標本のスキャン数を増加させるような回転式ポリゴンミラーへの入射角となるように回転式ポリゴンミラーと所定の位置関係で配置されていること、を特徴とする。
Claims (4)
- 標本を支持するイメージャステージと、
イメージャステージ上の標本をその視野内に捕らえるよう標本に近接配置された入射側開口及びそこから離れた場所にある出射側開口を有する光路と、
入射側開口を基準として複数に分かれて配置され且つそれぞれ輻射ビームを発する複数個の輻射源と、
2以上の反射面を持ち、各反射面は、各反射面に対応する各輻射源から入射する輻射ビームを反射して各輻射を標本の選択された領域の上に走査する回転式ポリゴンスキャナと、
標本に対する輻射ビームの作用によって生じ、入射側開口にて受光され、光路を介して出射側開口に伝送され、出射側開口から出射される光信号を各輻射源に対応づけて受光検知する光検知器システムと、
光検知器システムにより検知された光信号を各輻射源に対応づけて処理するプロセッサと、
を備える標本画像化用走査型イメージャであって、
各輻射源は、各輻射源に対応する回転式ポリゴンスキャナの各反射面によって反射される輻射ビームを発し、反射された各輻射ビームが各輻射ビームの走査方向と直角方向にずれた標本の位置を各輻射ビームの標本に対する作用時間をずらして順番に走査することができ、回転式ポリゴンスキャナの一回転あたりの標本のスキャン数を増加させるような回転式ポリゴンミラーへの入射角となるように回転式ポリゴンミラーと所定の位置関係で配置されていること、
を特徴とする標本画像化用走査型イメージャ。 - 請求項1記載の標本画像化用走査型イメージャにおいて、光信号の発生元が、別々の波長で蛍光を発する複数種類の蛍光プローブである標本画像化用走査型イメージャ。
- 請求項2記載の標本画像化用走査型イメージャにおいて、蛍光プローブにて発生する複数通りの光信号を、互いに他の光信号と干渉しない非干渉性光信号として光検知器システムにより受光検知させる標本画像化用走査型イメージャ。
- 標本を支持するイメージャステージと、イメージャステージ上の標本をその視野内に捕らえるよう標本に近接配置された入射側開口及びそこから離れた場所にある出射側開口を有する光路と、入射側開口を基準として複数に分かれて配置され且つそれぞれ輻射ビームを発する複数個の輻射源と、2以上の反射面を持ち、各反射面は、各反射面に対応する各輻射源から入射する輻射ビームを反射して各輻射を標本の選択された領域の上に走査する回転式ポリゴンスキャナと、標本に対する輻射ビームの作用によって生じ、入射側開口にて受光され、光路を介して出射側開口に伝送され、出射側開口から出射される光信号を各輻射源に対応づけて受光検知する光検知器システムと、光検知器システムにより検知された光信号を各輻射源に対応づけて処理するプロセッサと、を備える標本画像化用走査型イメージャであって、各輻射源は、各輻射源に対応する回転式ポリゴンスキャナの各反射面によって反射され、各輻射ビームが各輻射ビームの走査方向と直角方向にずれた標本の位置を各輻射ビームの標本に対する作用時間をずらして順番に走査することができ、回転式ポリゴンスキャナの一回転あたりの標本のスキャン数を増加させるような回転式ポリゴンミラーへの入射角となるように回転式ポリゴンミラーと所定の位置関係で配置されている標本画像化用走査型イメージャを準備するステップと、
複数の輻射ビームからの輻射を各輻射源に対応する回転式ポリゴンスキャナの各反射面で反射させて、回転式ポリゴンスキャナの一回転あたりのスキャン数を増加させるステップと、
標本上を直線経路に沿って、それぞれが異なる輻射波長を持つ反射された複数の輻射ビームを各輻射ビームの走査方向と直角方向にずれた標本の位置を各輻射ビームの標本に対する作用時間をずらして順番に掃引するステップと、
標本に対する各輻射ビームの作用により発生した光を集めるステップと、
集めた光を光路により所定の出射場所まで伝送させるステップと、
その出射場所にて且つ輻射ビームに対応づけてその集まった光を検知するステップと、
輻射ビームによる掃引と協調させつつ輻射ビームによる直線掃引経路に対し略直交する方向へと標本を移動させることにより複数の輻射ビームで順番に標本の掃引をラスタ的に行わせるステップと、
これら掃引動作、移動動作及び検知動作を協調させることによって標本の少なくとも一部を表す画素アレイを輻射ビームに対応づけて発生させるステップと、
を有する画像化方法。
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US11/018,759 | 2004-12-21 | ||
US11/018,759 US7286224B2 (en) | 2004-12-21 | 2004-12-21 | Time-multiplexed scanning light source for multi-probe, multi-laser fluorescence detection systems |
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JP4688667B2 true JP4688667B2 (ja) | 2011-05-25 |
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US20060132778A1 (en) | 2006-06-22 |
EP1674852B1 (en) | 2017-10-11 |
EP1674852A1 (en) | 2006-06-28 |
US7286224B2 (en) | 2007-10-23 |
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