JP4615618B2 - Reagent suction method - Google Patents

Reagent suction method Download PDF

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JP4615618B2
JP4615618B2 JP2010027151A JP2010027151A JP4615618B2 JP 4615618 B2 JP4615618 B2 JP 4615618B2 JP 2010027151 A JP2010027151 A JP 2010027151A JP 2010027151 A JP2010027151 A JP 2010027151A JP 4615618 B2 JP4615618 B2 JP 4615618B2
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reagent
lid
reagent container
opening
container
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信宏 喜多川
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Sysmex Corp
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Description

本発明は、検体自動分析装置における試薬吸引方法に関する。 The present invention relates to a reagent aspirating method in an automatic sample analyzer .

容器に収納された血清や血漿などの検体、反応試薬や希釈液などの測定試薬を、分注ピペットなどの液体分注装置で分注して試料を調製し分析することが一般に行われている。特に、血液凝固検査や免疫検査などの検査では、複数の検体及び測定試薬を連続的に分注して、その反応を測定する自動分析装置が用いられている。これらの自動分析装置は、各測定項目では試薬の消費量も依頼数もまちまちであるため、それらの試薬の交換時期も異なる。また、多数の検体をセットし、多数の項目を測定するために、測定開始から測定終了までに長時間を要することもある。そのため、分析装置に測定試薬や検体が長期間装置にセットされたままで使用されることがある。   In general, specimens such as serum and plasma stored in containers and measurement reagents such as reaction reagents and diluents are dispensed with a liquid dispensing device such as a dispensing pipette to prepare and analyze samples. . In particular, in tests such as blood coagulation tests and immunological tests, automatic analyzers that continuously dispense a plurality of specimens and measurement reagents and measure their reactions are used. Since these automatic analyzers vary in the amount of reagent consumption and the number of requests for each measurement item, the time for replacement of these reagents also differs. In addition, in order to set a large number of samples and measure a large number of items, it may take a long time from the start of measurement to the end of measurement. For this reason, the measurement reagent and the sample may be used in the analyzer for a long time.

そのように試薬や検体を分析装置に長期間セットしたままで放置しておくと、蒸発乾燥、埃等の混入などにより試薬の劣化が生じて分析結果に影響を及ぼすことになる。そこで、保存安定性を高めるために、容器の開口部を小さくしたり、容器収納部を保冷したりすることが行われている。しかし、それだけでは試薬や検体の劣化を防ぐことは難しい。   If the reagent or sample is left set in the analyzer for a long time as described above, the reagent will deteriorate due to evaporative drying, contamination with dust, etc., and the analysis result will be affected. Therefore, in order to increase the storage stability, the opening of the container is reduced or the container storage part is kept cold. However, it is difficult to prevent deterioration of reagents and specimens by itself.

そこで、試薬の劣化を防ぐために、試薬を吸引するとき以外は蓋で試薬容器の開口部を自動的に閉じる機構を設けた装置が提示されている。
（ａ）試薬容器の開口部に対応する部材を備えた機構部を分析装置に設け、当該機構部を自動的に動作させることで試薬容器の開口部を塞ぐ機能を備えた自動分析装置が、特許文献１、特許文献２、特許文献３などに開示されている。 Therefore, in order to prevent the deterioration of the reagent, there has been proposed an apparatus provided with a mechanism for automatically closing the opening of the reagent container with a lid except when the reagent is aspirated.
(A) An automatic analyzer having a function of closing a reagent container opening by providing a mechanism having a member corresponding to the opening of the reagent container and automatically operating the mechanism. It is disclosed in Patent Document 1 , Patent Document 2 , Patent Document 3 , and the like.

（ｂ）試薬容器として蓋を連結させた容器を用いて、当該蓋を引っかける部材を備えた回転機構部を分析装置に設け、当該回転機構を回転動作させて試薬容器の蓋を引き開ける機能を備えた自動分析装置が、特許文献４、特許文献５などに開示されている。 (B) Using a container having a lid connected as a reagent container, the analyzer is provided with a rotation mechanism portion having a member for hooking the lid, and the rotation mechanism is rotated to open the reagent container lid. The automatic analyzers provided are disclosed in Patent Literature 4 , Patent Literature 5 , and the like.

実開昭５７−１８５９６４号公報Japanese Utility Model Publication No. 57-185964 特開平８−１６００５０号公報JP-A-8-160050 特開平１０−３１１８３５号公報Japanese Patent Laid-Open No. 10-31835 特開平１−６１６６７号公報Japanese Unexamined Patent Publication No. 1-61667 特開平８−９４６２４号公報JP-A-8-94624

上記従来技術である（ａ）の装置では、容器の開口部を塞ぐ機構部を容器に対応させて構成させなくてはならず、分析装置の構成が制限される。そして、開口部を塞ぐ部材に試薬が付着した場合は次に使う試薬への汚染が起こりうる。また、容器と蓋とは別々であるので、測定の前後に蓋の取付け、取外しの必要があり、測定中にはその蓋を別の場所に保管しておかねばならず手間がかかる。   In the apparatus (a) of the prior art described above, the mechanism for closing the opening of the container must be configured to correspond to the container, and the configuration of the analyzer is limited. And when a reagent adheres to the member which plugs up an opening part, the contamination to the reagent used next may occur. Further, since the container and the lid are separate, it is necessary to attach and remove the lid before and after the measurement, and it is necessary to store the lid in another place during the measurement.

従来技術である上記（ｂ）の装置では、その問題点を解決するため、試薬容器に蓋を結合させている。しかし、自動分析装置には、回転機構部と、回転機構部に試薬容器の蓋を引っかけさせるための移動機構部とが必要となる。そのため、当該機構部の構造及びその動作制御が複雑になり、特に自動分析装置に、このような複雑な機構部を組み込むことは装置の大型化を招き、動作制御の複雑さから処理速度の低下を招くことになる。さらに、試薬容器の蓋は回転機構部によって開けられた後も開放状態を保持するように構成する必要があり、容器の構造が制限される。   In the conventional apparatus (b), a lid is coupled to the reagent container in order to solve the problem. However, the automatic analyzer requires a rotation mechanism unit and a moving mechanism unit for causing the rotation mechanism unit to hook the lid of the reagent container. This complicates the structure of the mechanism and its operation control. In particular, incorporating such a complicated mechanism in an automatic analyzer causes an increase in the size of the apparatus, and the processing speed decreases due to the complexity of the operation control. Will be invited. Furthermore, the lid of the reagent container needs to be configured to maintain an open state even after being opened by the rotation mechanism unit, and the structure of the container is limited.

本発明の目的は、検体自動分析装置において、長期間装置に試薬容器をセットした場合に生じる試薬の劣化を防止し、構造の簡単な試薬容器を使用して試薬容器の蓋の開閉を簡単に行える試薬吸引方法を提供することにある。 It is an object of the present invention to prevent reagent deterioration that occurs when a reagent container is set in the apparatus for a long period of time in an automatic sample analyzer, and to easily open and close the lid of the reagent container using a reagent container with a simple structure. It is an object of the present invention to provide a reagent suction method that can be performed .

本発明の液体吸引方法は、上方に突出した爪部を有すると共に端部の回転支点により開閉可能な略円形状の蓋を備えた試薬容器を設置するための試薬容器設置部、試薬容器の蓋を開閉するための開閉部材および分注ピペットを備え、試薬容器に収容された試薬を用いて血清や血漿の検査を行う検体自動分析装置における試薬吸引方法であって、略円形状の蓋により試薬容器の開口部を密閉可能な試薬容器を使用し、開閉部材および試薬容器の水平方向の相対移動に伴い開閉部材と爪部が当接して試薬容器の回転支点側へ爪部が移動することにより略円形状の蓋を上方に開放させ、前記相対移動を停止させ、分注ピペットを下降させて蓋が開放した状態の試薬容器の開口部から分注ピペットによって試薬を吸引し、分注ピペットを上昇させた後、前記相対移動とは逆方向の相対移動に伴い開閉部材と開放状態の略円形状の蓋が当接して略円形状の蓋によって試薬容器の開口部を閉じることを特徴とする試薬吸引方法である。 A liquid suction method according to the present invention includes a reagent container installation portion for installing a reagent container having a claw portion protruding upward and having a substantially circular lid that can be opened and closed by a rotation fulcrum of an end portion, and a lid of the reagent container A reagent aspirating method in an automatic sample analyzer that includes an opening / closing member for opening and closing and a dispensing pipette, and tests a serum or plasma using a reagent contained in a reagent container, wherein the reagent is formed by a substantially circular lid By using a reagent container that can seal the opening of the container , the claw is moved toward the rotation fulcrum side of the reagent container by contacting the claw with the opening / closing member along with the horizontal movement of the opening / closing member and the reagent container. The substantially circular lid is opened upward, the relative movement is stopped, the dispensing pipette is lowered, the reagent is aspirated from the opening of the reagent container with the lid opened, and the dispensing pipette is removed. After raising Is a reagent aspirating method characterized by closing the opening of the reagent container by a substantially circular lid substantially circular lid abuts the open state and the opening and closing member with the movement in the opposite direction relative to the said relative movement .

本明細書における分注ピペットとは、ピペット本体及びピペット本体に接続されたシリンジあるいはポンプ等の液定量手段を有し、例えば、自動分析装置においては、下記のように動作するものが好ましい。すなわち、ピペット本体が吸引元である検体容器あるいは試薬容器内に挿入されると、ピペット本体に接続された液定量手段が吸引動作を開始し一定量の検体あるいは試薬を分取し、次に、ピペット本体は吐出先である反応容器に向かって移動され反応容器内に挿入されると、液定量手段が吐出動作を開始し、所定量の検体あるいは試薬を分注する。次に、このピペット本体に洗浄水やエアーが供給され先端から吐出される。あるいは、逆に洗浄水を先端から吸引し排出する。このようにして洗浄されたピペット本体は再び検体容器、試薬容器、反応容器の間を相対移動する。 The pipetting pipette in the present specification has a pipette body and a liquid quantification means such as a syringe or a pump connected to the pipette body. For example, an automatic analyzer preferably operates as follows. That is, when the pipette body is inserted into the specimen container or reagent container that is the source of the suction, the liquid quantification means connected to the pipette body starts the suction operation and dispenses a certain amount of specimen or reagent. When the pipette body is moved toward the reaction container which is the discharge destination and inserted into the reaction container, the liquid quantification means starts the discharge operation and dispenses a predetermined amount of sample or reagent. Next, cleaning water or air is supplied to the pipette body and discharged from the tip. Alternatively, the cleaning water is sucked and discharged from the tip. The pipette main body thus cleaned is relatively moved again between the sample container, the reagent container, and the reaction container.

本明細書におけるピペット昇降手段とは、プーリに捲回されたベルト、あるいはボールねじ等の駆動によりピペットを垂直方向に精密移動できるものが好ましい。この発明における基台移動手段とは、プーリに捲回されたベルト、あるいはボールねじ等の駆動により基台を水平方向に精密移動できるものが好ましい。 The pipette lifting / lowering means in this specification is preferably one that can precisely move the pipette in the vertical direction by driving a belt wound around a pulley or a ball screw. The base moving means in the present invention is preferably one that can precisely move the base in the horizontal direction by driving a belt wound around a pulley or a ball screw.

本明細書における試薬容器設置部とは、試薬容器を載置する部位をいう。必要に応じて試薬容器設置部も移動できるものが用いられる。 The reagent container installation section in the present specification, refers to a site for mounting the reagent container. A reagent container installation unit that can be moved as necessary is used.

本明細書における試薬容器とは上部に開口部を持つ容器で、回転支点によって開閉できる蓋が結合され、その蓋は試薬容器の開口部を塞ぐように構成される。蓋は試薬容器を密閉できるような構造に構成されることが好ましい。 The reagent container in this specification is a container having an opening at the top, and a lid that can be opened and closed by a rotation fulcrum is coupled, and the lid is configured to close the opening of the reagent container. The lid is preferably constructed in a structure that can seal the reagent container.

蓋に形成される爪部とは、蓋の外側に突出した部位をいう。そして、蓋を開放した状態を保持するために、蓋の回転支点側から見て爪部と蓋との間にＶ字状などの谷部が形成される。そのため、爪部は蓋の回転支点寄りから突出されるように形成されることが好ましい。爪部の形状は分注機構部の開閉部材である突出部の形状と対応できるように適宜選択される。 The nail | claw part formed in a lid means the site | part which protruded to the outer side of the lid | cover. And in order to hold | maintain the state which open | released the lid | cover, trough parts, such as V shape, are formed between a nail | claw part and a lid seeing from the rotation fulcrum side of a lid | cover. Therefore, it is preferable that the claw portion is formed so as to protrude from the rotation fulcrum of the lid. The shape of the claw portion is appropriately selected so as to correspond to the shape of the protruding portion which is an opening / closing member of the dispensing mechanism portion.

試薬容器は試薬容器設置部に設置された際に蓋の開閉方向が方向づけられるように構成される。例えば、容器本体と蓋とが一体に形成されたものや、一体でなくても蓋を容器本体に一方向にしか取り付けられないものを用いた場合には、試薬容器設置部に対して試薬容器を一方向にしか設置できないように試薬容器及び試薬容器設置部の形状が適宜選択される。蓋を容器本体に取り付けた際に蓋の相対的方向が決定されないものであれば、例えば、複数の試薬容器が蓋を連結することができる構造にすることで、試薬容器を設置した際には蓋の開閉方向を方向づけることができる。 The reagent container is configured such that the opening and closing direction of the lid is oriented when the reagent container is installed in the reagent container installation unit. For example, when the container body and the lid are integrally formed, or the container body and the lid that can be attached to the container body only in one direction even if they are not integral, the reagent container with respect to the reagent container installation portion The shape of the reagent container and the reagent container installation part is appropriately selected so that the container can be installed only in one direction. If the relative direction of the lid is not determined when the lid is attached to the container body, for example, when a reagent container is installed by using a structure in which a plurality of reagent containers can be connected to the lid. The opening and closing direction of the lid can be oriented.

この明細書における分注機構部の基台に設けられた突出部とは、分注機構部の試薬容器に対する相対移動に伴い蓋の爪部を蓋の回転支点と逆側から当てて、基台を蓋の回転支点側に移動することで蓋の爪部を押し当て蓋を開放し、蓋と蓋の爪部の間に入ることで蓋の開放状態とし、相対移動とは逆方向の相対移動に伴い開放状態の蓋を回転支点側とは逆側へ押し当て移動することにより蓋を閉じることができる部位のことである。形状は蓋及び蓋の爪部と適合するように選択されれば、基台に単なる突起を設けるだけでも構わない。あるいは、通常は後退しており必要なときだけ突出するよう構成すれば分注機構部及び試薬の配置や構造に自由度が高くなる。例えば、エアシリンダやソレノイドを用いてピストンを突出部として上下に往復直線移動させることができる。 In the present specification, the protrusion provided on the base of the dispensing mechanism portion refers to the base by applying the claw portion of the lid from the side opposite to the rotation fulcrum of the lid as the dispensing mechanism portion moves relative to the reagent container. Is moved to the rotation fulcrum side of the lid, pressing the claw part of the lid to release the lid, and entering between the lid and the claw part of the lid, the lid is opened , and the relative movement in the direction opposite to the relative movement Accordingly, the lid can be closed by pressing and moving the opened lid to the side opposite to the rotation fulcrum side . If the shape is selected so as to be compatible with the lid and the claw portion of the lid, a simple protrusion may be provided on the base. Or if it is usually set back so that it protrudes only when necessary, the degree of freedom is increased in the arrangement and structure of the dispensing mechanism and reagent. For example, an air cylinder or a solenoid can be used to reciprocate linearly up and down using a piston as a protrusion.

本発明の試薬吸引方法によれば、試薬容器の蓋の爪部を利用して分注ピペットによって試薬を吸引する際に簡単に蓋の開閉を行うことができる。試薬容器の蓋開閉方向が方向づけられるように構成された試薬容器を用いる場合には、試薬容器の開閉動作をより単純化できる。 According to the reagent aspirating method of the present invention, the lid can be easily opened and closed when the reagent is aspirated by a dispensing pipette using the claw portion of the lid of the reagent container . When using a reagent container configured such that the opening / closing direction of the reagent container is oriented, the opening / closing operation of the reagent container can be further simplified.

本実施形態の試薬吸引方法を実施する自動分析装置の要部斜視図である。It is a principal part perspective view of the automatic analyzer which implements the reagent suction method of this embodiment . 本実施形態の試薬吸引方法を実施する自動分析装置の要部ブロック図である。It is a principal part block diagram of the automatic analyzer which implements the reagent suction method of this embodiment . 本実施形態の試薬吸引方法に用いられる試薬ユニットの一例の斜視図である。It is a perspective view of an example of the reagent unit used for the reagent suction method of this embodiment . 本実施形態の試薬吸引方法に用いられる試薬ユニットの一例の分解状態を示す斜視図である。It is a perspective view which shows the decomposition | disassembly state of an example of the reagent unit used for the reagent suction method of this embodiment . 本実施形態の試薬吸引方法に用いられる試薬容器の一例において開口部が密閉されている状態を示す斜視図である。It is a perspective view which shows the state by which the opening part is sealed in an example of the reagent container used for the reagent suction method of this embodiment . 本実施形態の試薬吸引方法に用いられる試薬容器の一例において開口部が開放されている状態を示す斜視図である。It is a perspective view which shows the state by which the opening part is open | released in an example of the reagent container used for the reagent suction method of this embodiment . 本実施形態の試薬吸引方法における試薬容器の蓋の開閉動作を示す図である。It is a figure which shows the opening / closing operation | movement of the lid | cover of a reagent container in the reagent suction method of this embodiment . 本実施形態の試薬吸引方法を実施する自動分析装置の突出部の他の例を示す図である。It is a figure which shows the other example of the protrusion part of the automatic analyzer which implements the reagent suction method of this embodiment .

以下図面を用いて本発明の実施例について説明をする。図１は本発明の実施の一形態による自動分析装置の要部を示す斜視図である。反応系、測定系などの基本構成は従来から知られている装置と同じである。自動分析装置１０は分注機構部１２、検体設置部１４、試薬設置部１６、反応容器設置部１８及び測定部２０から主に構成される。分注機構部１２は、基台２２に備えられ分注ピペット２４を昇降させる分注ピペット昇降部２６と、基台２２をＸＹ軸の２軸方向に移動させる基台移動部２８から構成される。   Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a perspective view showing a main part of an automatic analyzer according to an embodiment of the present invention. The basic configuration of the reaction system, measurement system, etc. is the same as that of conventionally known apparatuses. The automatic analyzer 10 is mainly composed of a dispensing mechanism unit 12, a sample installing unit 14, a reagent installing unit 16, a reaction container installing unit 18 and a measuring unit 20. The dispensing mechanism unit 12 includes a dispensing pipette lifting / lowering unit 26 that is provided on the base 22 and moves the dispensing pipette 24 up and down, and a base moving unit 28 that moves the base 22 in the biaxial directions of the XY axes. .

分注ピペット２４はその上端部において保持部３０により保持され、分注ピペットの上端はチューブを介して液定量部３１（図２参照）に接続されている。液定量部３１は、ここではモータで駆動されるシリンジとした。また、シリンジには洗浄液を供給するための洗浄液チャンバがバルブを介して接続されている。   Dispensing pipette 24 is held by holding section 30 at its upper end, and the upper end of the dispensing pipette is connected to liquid quantifying section 31 (see FIG. 2) via a tube. The liquid determination unit 31 is a syringe driven by a motor here. Further, a cleaning liquid chamber for supplying a cleaning liquid is connected to the syringe through a valve.

ピペット昇降部２６は、基台２２において上下方向に離れて設けられた一対のプーリ３２、３４と、プーリ３２、３４の一方３２を回転駆動するモータ３５と、プーリ３２、３４間に掛け渡されたベルト３６とを備え、ピペット２４の保持部３０をベルト３６に固着することにより構成されている。   The pipette elevating part 26 is stretched between a pair of pulleys 32, 34 provided apart in the vertical direction on the base 22, a motor 35 that rotationally drives one of the pulleys 32, 34, and the pulleys 32, 34. Belt 36 and the holding part 30 of the pipette 24 is fixed to the belt 36.

突出部は、エアシリンダ４０のピストン３８からなる。エアシリンダ４０は外部から供給される作動エアーによりピストン３８が突出した状態と引込んだ状態とを作り出す。   The protruding portion is composed of a piston 38 of the air cylinder 40. The air cylinder 40 creates a state in which the piston 38 protrudes and a state in which the piston 38 is retracted by operating air supplied from the outside.

図２は、自動分析装置１０の要部のブロック図である。４２は、ＣＰＵ，ＲＯＭ，ＲＡＭ，タイマー，カウンター等を有するマイクロコンピュータを含む制御部である。制御部４２には、ピペット昇降部２６、液定量部３１、基台移動部２８、突出部３８の駆動源４０、各種位置決めセンサ等が接続され、さらに、キー入力可能な操作部４４や測定結果等を表示する出力部４６が接続されている。   FIG. 2 is a block diagram of a main part of the automatic analyzer 10. A control unit 42 includes a microcomputer having a CPU, ROM, RAM, timer, counter, and the like. The control unit 42 is connected to the pipette elevating unit 26, the liquid quantifying unit 31, the base moving unit 28, the driving source 40 of the projecting unit 38, various positioning sensors, and the like. Etc. are connected.

図３は本発明に用いられる、複数の試薬容器５０、５６、５８からなる試薬ユニット６０の斜視図である。図４は試薬ユニットの分解状態を示す斜視図である。また図５は試薬容器の開口部５９が蓋５２により密閉されている状態を示し、図６は試薬容器の開口部５９が開放されている状態を示す。試薬容器の蓋５２は容器本体５１に取付けられた支持体５３の回転支点５４によって回転し開閉可能な構造となっている。蓋５２の上面には爪部５５が設けられている。なお、蓋５２、支持体５３、回転支点５４、爪部５５、及び後述する嵌合部６２、６４の構造は試薬容器５０、５６、５８において共通のものとなっている。試薬容器５０の蓋支持体５３には隣接する試薬容器５６、５８の蓋支持体と結合できるように嵌合部６２、６４を備えている。２つ以上の試薬容器を相互結合させて試薬ユニットとして試薬設置部１６に収納することによって、各容器の蓋の開閉方向は規定されることになる。また、蓋５２は容器本体５１側へ強く押さえつけることで容器本体５１の開口部５９を密閉するように構成されている。そして、この状態で試薬ユニット６０を保冷庫に保存する。装置に設置するときは手で密閉状態を解除し、蓋の自重で容器本体の開口部５９を閉じている状態にしておく。   FIG. 3 is a perspective view of a reagent unit 60 comprising a plurality of reagent containers 50, 56, 58 used in the present invention. FIG. 4 is a perspective view showing an exploded state of the reagent unit. 5 shows a state in which the opening 59 of the reagent container is sealed by the lid 52, and FIG. 6 shows a state in which the opening 59 of the reagent container is opened. The lid 52 of the reagent container has a structure that can be opened and closed by rotating by a rotation fulcrum 54 of a support 53 attached to the container main body 51. A claw portion 55 is provided on the upper surface of the lid 52. The structure of the lid 52, the support 53, the rotation fulcrum 54, the claw portion 55, and the fitting portions 62 and 64 described later are common to the reagent containers 50, 56, and 58. The lid support 53 of the reagent container 50 is provided with fitting portions 62 and 64 so that it can be coupled with the lid support of the adjacent reagent containers 56 and 58. By opening two or more reagent containers to each other and storing them as reagent units in the reagent installing section 16, the opening and closing directions of the lids of the containers are defined. Further, the lid 52 is configured to seal the opening 59 of the container body 51 by strongly pressing it against the container body 51 side. In this state, the reagent unit 60 is stored in a cold storage. When installing in the apparatus, the sealed state is released by hand, and the opening 59 of the container body is closed by the weight of the lid.

本実施例の自動分析装置１０の動作について説明する。まず、基台移動部２８によって基台２２は検体載置部１４に移動し、分注ピペット２４が下降して検体容器の検体を所定量吸引する。その後、分注ピペット２４は上昇して基台２２は反応容器設置部１８に水平移動して、分注ピペット２４が下降して吸引した検体を分注する。   The operation of the automatic analyzer 10 of this embodiment will be described. First, the base 22 is moved to the sample mounting unit 14 by the base moving unit 28, and the dispensing pipette 24 is lowered to suck a predetermined amount of the sample in the sample container. Thereafter, the dispensing pipette 24 is raised and the base 22 is moved horizontally to the reaction vessel setting section 18, and the dispensing pipette 24 is lowered to dispense the aspirated sample.

次に、図７（ａ）から（ｆ）を参照しつつ、試薬の吸引動作について説明する。図７は蓋開閉動作を説明するための図である。
（ａ）基台２２は試薬設置部１６に向かって移動する（図では左方向に移動する）。試薬設置部１６に到達する前にエアシリンダ４０のピストン３８を突出させておく。
（ｂ）基台２２の水平移動に伴い突出部３８が試薬容器５０の爪部５５に当接し左方向に押圧することにより蓋５２を回転支点５４の周りに反時計方向に回転させて蓋５６を開ける。
（ｃ）基台２２が停止し、分注ピペット２４が下降し試薬容器５０から試薬を定量採取する。その間、突出部３８は爪部５５と蓋５２の上面とにより形成されるＶ字状谷部５７に位置するため、蓋５２は容器開口部５９を開放した状態で保持されることになる。
（ｄ）定量採取が終了すると分注ピペット２４は上昇する。
（ｅ）基台２２の右方向への移動に伴い突出部３８が試薬容器５０の蓋５２の上面に当接し右方向に押圧することにより蓋５２を回転支点５４の周りに時計方向に回転させて蓋５２を閉じる。
（ｆ）エアシリンダ４０のピストン３８を縮退させる。基台２２は反応容器設置部１８に移動して吸引した試薬を検体中に分注する。 Next, the reagent aspirating operation will be described with reference to FIGS. FIG. 7 is a diagram for explaining the lid opening / closing operation.
(A) The base 22 moves toward the reagent installing unit 16 (moves leftward in the figure). The piston 38 of the air cylinder 40 is protruded before reaching the reagent installing portion 16.
(B) With the horizontal movement of the base 22, the protrusion 38 comes into contact with the claw portion 55 of the reagent container 50 and presses leftward to rotate the lid 52 around the rotation fulcrum 54 in the counterclockwise direction. Open.
(C) The base 22 is stopped, the dispensing pipette 24 is lowered, and the reagent is quantitatively collected from the reagent container 50. Meanwhile, since the protruding portion 38 is located in the V-shaped valley portion 57 formed by the claw portion 55 and the upper surface of the lid 52, the lid 52 is held with the container opening 59 open.
(D) When the quantitative sampling is completed, the dispensing pipette 24 is raised.
(E) As the base 22 moves to the right, the protrusion 38 contacts the upper surface of the lid 52 of the reagent container 50 and presses it to the right, thereby rotating the lid 52 around the rotation fulcrum 54 in the clockwise direction. Then close the lid 52.
(F) The piston 38 of the air cylinder 40 is retracted. The base 22 moves to the reaction container setting unit 18 and dispenses the aspirated reagent into the sample.

以上、突出部が伸縮する例について説明したが、図８に示すように伸縮しない棒状部材６６であってもよい。基本的な機能は同じである。   As described above, the example in which the projecting portion expands and contracts has been described. However, as shown in FIG. The basic functions are the same.

本発明に用いられる自動分析装置は、分注機構部の基部に突出部を付加し、容器の蓋に爪部を付加したものであり、簡単な構造で蓋の開閉を行える。また、分注の動作についても特に変更を要しないので動作制御も容易であり処理速度を低下させる恐れもない。   The automatic analyzer used in the present invention is such that a protrusion is added to the base of the dispensing mechanism and a claw is added to the lid of the container, and the lid can be opened and closed with a simple structure. Also, since no particular change is required for the dispensing operation, the operation control is easy and there is no fear of reducing the processing speed.

また、試薬容器の蓋は開放した状態で保持されるので分注ピペットの吸引中に蓋が閉じるという問題も発生しない。   Further, since the lid of the reagent container is held in an open state, there is no problem that the lid closes during suction of the dispensing pipette.

なお前記実施例では、試薬容器は蓋を開口部に押し付ければ密閉状態となるような構造となっている。そこで図７（ｅ）において、基台２２の水平移動に伴う突出部３８の移動により蓋５２を時計方向に回転させて閉じた後、突出部３８が蓋５２を下方向に押圧するよう制御すれば、自動的に試薬容器を密閉することが可能である。測定動作終了時にこの制御を行うことで、試薬の乾燥・劣化をより防ぐことができる。また測定再開時には、基台２２の水平移動に伴う突出部３８の移動により、蓋５２の密閉状態を解除し反時計方向に回転させて開けるよう構成することもできる。全ての測定が終了した時に、前記のように突出部３８によって蓋５２を下方向に押圧して自動的に試薬容器の密閉を行えば、自動分析装置から取り出した試薬容器を、内容物をこぼすおそれなく、そのまま保冷庫に保存することができる。   In the above-described embodiment, the reagent container is structured to be sealed when the lid is pressed against the opening. Therefore, in FIG. 7E, after the protrusion 52 is moved by the horizontal movement of the base 22, the lid 52 is rotated clockwise and closed, and then the protrusion 38 presses the lid 52 downward. In this case, the reagent container can be automatically sealed. By performing this control at the end of the measurement operation, it is possible to further prevent the reagent from being dried and deteriorated. Further, when the measurement is resumed, the cover 52 can be released from the sealed state by rotating the protruding portion 38 accompanying the horizontal movement of the base 22 and can be rotated counterclockwise to be opened. When all the measurements are completed, if the lid 52 is pressed downward by the protruding portion 38 and the reagent container is automatically sealed as described above, the contents of the reagent container taken out from the automatic analyzer are spilled. Without fear, it can be stored in the cool box as it is.

１２ 分注機構部
１４ 検体設置部
１６ 試薬設置部
１８ 反応容器設置部
２０ 測定部
２２ 基台
２４ 分注ピペット
２６ 分注ピペット昇降部
２８ 基台移動部
３８、６６ 突出部
５０、５６、５８ 試薬容器
５２ 蓋
５４ 回転支点
５５ 爪部 12 Dispensing mechanism part 14 Specimen installing part 16 Reagent installing part 18 Reaction container installing part 20 Measuring part 22 Base 24 Dispensing pipette 26 Dispensing pipette lifting part 28 Base moving part 38, 66 Protruding part 50, 56, 58 Reagent Container 52 Lid 54 Rotation fulcrum 55 Claw

Claims (4)

上方に突出した爪部を有すると共に端部の回転支点により開閉可能な略円形状の蓋を備えており内部に試薬を収容した試薬容器を設置するための試薬容器設置部、試薬容器の蓋を開閉するための開閉部材および分注ピペットを備え、試薬容器に収容された試薬を用いて血清や血漿の検査を行う検体自動分析装置における試薬吸引方法であって、
略円形状の蓋により試薬容器の開口部を密閉可能な試薬容器を使用し、
開閉部材および試薬容器の水平方向の相対移動に伴い開閉部材と爪部が当接して試薬容器の回転支点側へ爪部が移動することにより略円形状の蓋を上方に開放させ、
前記相対移動を停止させ、分注ピペットを下降させて蓋が開放した状態の試薬容器の開口部から分注ピペットによって試薬を吸引し、
分注ピペットを上昇させた後、前記相対移動とは逆方向の相対移動に伴い開閉部材と開放状態の略円形状の蓋が当接して略円形状の蓋によって試薬容器の開口部を閉じることを特徴とする試薬吸引方法。 A reagent container installation part for installing a reagent container containing a reagent therein, having a claw part protruding upward and having a substantially circular lid that can be opened and closed by a rotation fulcrum of the end part, and a reagent container cover A reagent aspirating method in an automatic sample analyzer that includes an open / close member for opening and closing and a dispensing pipette, and performs a test of serum or plasma using a reagent contained in a reagent container ,
Use a reagent container that can seal the opening of the reagent container with a substantially circular lid,
With the horizontal movement of the opening and closing member and the reagent container, the opening and closing member and the claw part come into contact with each other and the claw part moves to the rotation fulcrum side of the reagent container, thereby opening the substantially circular lid upward,
The relative movement is stopped, the dispensing pipette is lowered, and the reagent is aspirated by the dispensing pipette from the opening of the reagent container with the lid open.
After raising the dispensing pipette, the opening / closing member and the substantially circular lid in the open state come into contact with the relative movement in the direction opposite to the relative movement, and the opening of the reagent container is closed by the substantially circular lid . A reagent suction method characterized by the above. 開閉部材または試薬容器設置部を移動することにより、蓋の開閉を行うことを特徴とする請求項１記載の試薬吸引方法。   The reagent suction method according to claim 1, wherein the lid is opened and closed by moving the opening / closing member or the reagent container installing portion. 試薬容器を容器設置部に設置した際に、試薬容器の蓋開閉方向が方向づけられるように構成された試薬容器を用いることを特徴とする請求項１または請求項２に記載の試薬吸引方法。   The reagent aspirating method according to claim 1 or 2, wherein the reagent container is configured so that the opening and closing direction of the reagent container is oriented when the reagent container is installed in the container installation unit. 爪部が略円形状の蓋の回転支点の近傍に設けられていることを特徴とする請求項１〜３の何れか１項に記載の試薬吸引方法。The reagent suction method according to any one of claims 1 to 3, wherein the claw portion is provided in the vicinity of a rotation fulcrum of the substantially circular lid.
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