JP4378642B2 - Nutritional functional food derived from Astragalus fish - Google Patents
Nutritional functional food derived from Astragalus fish Download PDFInfo
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本発明は、ゲンゲ科魚類に由来する栄養機能性食品に関し、特に、抗酸化、抗高血圧及び老化防止のためのゲンゲ科魚類に由来する栄養機能性材料を有効成分として含んでいる固形製剤の形の栄養機能性食品に関するものである。 The present invention relates to a nutritional functional food derived from Astragalus fish, and in particular, in the form of a solid preparation containing as an active ingredient a nutritional functional material derived from Astragalus fish for antioxidant, antihypertensive and anti-aging purposes. It is related to nutritional functional foods.
現在盛んに研究されている栄養機能性材料は、様々な疾患を予防する効果を有するため、膨大な負担となっている医療費の削減が期待でき、また、日常の健康をサポートする可能性を持っている。中でも、従来から食されている海洋性の原材料は研究によって有効な機能性を有していたとの報告も多々あり、現在も更なる栄養機能性材料の探索が望まれている。 Nutritional functional materials that are being actively researched have the effect of preventing various diseases, so it is possible to expect a reduction in medical costs, which is an enormous burden, and also to support the possibility of daily health. have. In particular, there are many reports that marine raw materials that have been eaten in the past have had effective functionalities through research, and further search for nutritional functional materials is still desired.
また、魚類のような海洋性の原材料を利用するにはいくつかの問題がある。例えばコラーゲンのような特定の成分は皮膚や腱のような組織に多く含まれているが、ウシやブタのような大型動物では皮膚を他の組織や器官から分別して取出すのは容易であっても、魚類ではそれが困難である。 There are also several problems with using marine raw materials such as fish. For example, certain components such as collagen are abundant in tissues such as skin and tendons, but in large animals such as cattle and pigs, it is easy to separate and remove skin from other tissues and organs. But it is difficult for fish.
そのため魚類からそのような組織を分別することなくコラーゲンのような特定の成分を抽出する方法として、特開２００４−９９５６９号公報に開示されているような非ウシ起源変性コラーゲンの製造方法が開発されているが、この方法は、骨、筋肉、内臓を含む魚体全体を原料に使用するだけものであって、魚体全体から得られる栄養機能性材料を有効成分として製品化するものではなく、また、人体に対する機能や効果も解明されていなかった。また、その製造工程も未だ複雑なものであった。 Therefore, as a method for extracting specific components such as collagen from fish without separating such tissues, a method for producing non-bovine-derived modified collagen as disclosed in JP-A-2004-99659 has been developed. However, this method only uses the whole fish body including bones, muscles, and internal organs as a raw material, and does not commercialize a nutritional functional material obtained from the whole fish body as an active ingredient. Functions and effects on the human body have not been elucidated. In addition, the manufacturing process is still complicated.
本発明者は、原料魚種としてゲンゲ科魚類に着目した。この魚種は富山湾周辺で漁猟の際に外道としてかなりの漁獲量があるものの、一部が地元で食用に供される他は流通市場に出荷されることはなく、殆どが廃棄処分されているものであるが、その機能性の確認及び研究を行ったところ、抗酸化作用、抗高血圧作用、老化防止作用、学習亢進に役立つ優れた栄養機能性成分を含んでいることを見出した。 The present inventor has paid attention to the genus fish as a raw fish species. Although this fish species has a considerable catch as an outer road in the vicinity of Toyama Bay, some of it is used locally for food and is not shipped to the secondary market. However, as a result of confirming and researching its functionality, it was found that it contains an excellent nutritional functional component useful for antioxidant action, antihypertensive action, anti-aging action, and learning enhancement.
そこで本発明者は、上述のとおりその利用が限定されている「げんげ」を有効利用すること、さらにはゲンゲ科魚類の骨、筋肉、内臓を含む魚体全体を利用した栄養機能性食品の開発を目的として、先に述べたような魚体組織の分別を必要とせず、かつゲンゲ科魚類の各種栄養機能を阻害することがない比較的簡単なゲンゲ科魚類に由来する栄養機能性材料の製造方法およびその製剤方法により、常備形態に適したゲンゲ科魚類由来の栄養機能性材料を有効成分として含んでいる固形製剤の形をした食品を開発することに成功した。 Therefore, the present inventor makes effective use of “genge” whose use is limited as described above, and further develops a nutritional functional food using the whole fish body including bones, muscles and internal organs of the genus fish. As a purpose, a method for producing a nutritional functional material derived from a relatively simple genus fish that does not require the separation of fish tissues as described above and does not inhibit various nutritional functions of the genus With this formulation method, we have succeeded in developing a food product in the form of a solid preparation containing as an active ingredient a nutrient functional material derived from a genus fish suitable for a regular form.
本発明の有効成分であるゲンゲ科魚類に由来する栄養機能性材料は、ゲンゲ科魚類の魚体を洗浄し、選別する工程、選別された魚体の全部または一部を適当なサイズにカットしてミンチ状の試料にする工程、ミンチ状の試料を水中で熱処理する工程、熱処理された試料をフリーズ・ドライする工程、フリーズ・ドライされた試料を粉砕する工程によって、栄養機能性を有するゲンゲ科魚類由来のフリーズ・ドライ粉末材料として得られる。 The nutrient functional material derived from the genus fish, which is the active ingredient of the present invention, is a process of washing and selecting the genus fish and cutting the whole or part of the selected fish into an appropriate size. Derived from Astragalus fish with nutritional functionality through the process of forming a sample in the form of a powder, the process of heat-treating a minced sample in water, the process of freeze-drying the heat-treated sample, and the process of crushing the freeze-dried sample Obtained as a freeze-dried powder material.
フリーズ・ドライとは、熱処理することなく試料を脱水することができ、そのために含有成分の熱変性を防ぐことができる。この結果、本発明により得られる栄養機能性材料は、抗酸化作用、抗高血圧作用、老化防止作用および学習亢進に役立つゲンゲ科魚類由来の栄養機能性成分を高い含有率で含ませることができる。 Freeze-drying allows the sample to be dehydrated without heat treatment, thereby preventing thermal denaturation of the contained components. As a result, the nutritional functional material obtained by the present invention can contain a nutritional functional component derived from a genus fish that is useful for antioxidant, antihypertensive, anti-aging, and learning enhancement at a high content.
ゲンゲ科の魚類には、代表的な種類としてヨコスジクロゲンゲ、サラサガジ、コグチヘビゲンゲ、カンテンゲンゲ、シロゲンゲおよびアシナガゲンゲの６種類が知られ、そのうちカンテンゲンゲが一般に「げんげ」と呼ばれている魚種である。これらの魚類は水深２００ｍ以上の大陸棚斜面に生息し、一部は食用に供されることもあるが大部分は漁獲されても廃棄されている。ゲンゲ科の魚類はすべて本発明に使用することができるが、「げんげ」の通称で知られているカンテンゲンゲが入手し易い。 There are six typical species of genus fish: Yokosuji Kurogenge, Sarasagaji, Koguchibigege, Kantengage, Shirogenge and Ashinga Genge, of which Kantengage is generally called “genge” It is. These fish live on continental shelf slopes with a depth of 200 m or more, and some are used for food, but most are discarded even if caught. All the genus fish can be used in the present invention, but the common agate known as “Genge” is easily available.
本発明において使用されるゲンゲ科魚類由来の栄養機能性材料は、骨、筋肉、内臓を含む魚体全体を原料に使用することができ、さらには魚体組織の特定部位のみを原料にすることで、特定の機能性を高めた栄養機能性材料を得ることもできる。 The nutritional functional material derived from the genus fish used in the present invention can use the whole fish body including bones, muscles, and internal organs as a raw material, and further by using only a specific part of the fish tissue as a raw material, It is also possible to obtain a nutritional functional material with an increased specific functionality.
例えば、洗浄・選別により魚体から表面組織を剥離させて、コラーゲンに富むゲンゲ科魚類の表面組織のみを原材料として上記の製造方法にしたがって製造した場合は、化粧品や健康食品等で多く使用されるコラーゲン主体の栄養機能性組成物材料を得ることができる。 For example, when the surface tissue is peeled off from the fish body by washing and sorting and only the surface tissue of the genus genus fish rich in collagen is produced as a raw material according to the above production method, collagen often used in cosmetics, health foods, etc. A main nutritional functional composition material can be obtained.
また、コエンザイムＱ１０、γ−トコフェノール、ＤＨＡ（ドコサヘキサエン酸）、食用脂肪油およびグリセリン脂肪酸エステルなどのを添加することによって、抗酸化作用、抗高血圧作用、老化防止作用および学習亢進機能を相乗的に高めたゲンゲ科魚類由来の栄養機能性材料を得ることもできる。 In addition, by adding coenzyme Q10, γ-tocophenol, DHA (docosahexaenoic acid), edible fatty oil, glycerin fatty acid ester, and the like, the antioxidant action, antihypertensive action, antiaging action and learning enhancement function are synergistically It is also possible to obtain a nutritional functional material derived from an enhanced genus fish.
このようにして製造されたゲンゲ科魚類由来の栄養機能性材料は、そのままの形態で一般食品等に簡便に配合することもできるが、本発明ではカプセル化、錠剤化、タブレット化又はカプレット化することにより、常備携帯に適した形状にしたゲンゲ科魚類由来の栄養機能性材料を有効成分として含んでいる固形製剤の形をした食品を提供するものである。 The nutritive functional material derived from the genus fish thus produced can be simply blended into a general food or the like in the form as it is, but in the present invention, it is encapsulated, tableted, tableted or capletted. Accordingly, the present invention provides a food in the form of a solid preparation containing as an active ingredient a nutritional functional material derived from Astragalus fish that has a shape suitable for regular carrying.
なお、錠剤化などによる常備携帯に適した形状とする場合は、慣用の賦形剤、崩壊剤などをさらに添加することもでき、また、カプセル化もソフト・カプセルに限定されるものではなく、ハード・カプセルであってもよい。 In the case of a shape suitable for regular carrying such as tableting, conventional excipients, disintegrants and the like can be further added, and encapsulation is not limited to soft capsules, It may be a hard capsule.
本発明によるゲンゲ科魚類由来の栄養機能性食品は、ゲンゲ科魚類由来の栄養機能（成分）を失うことなく有効に含んでおり、その結果、ゲンゲ科魚類由来の栄養機能性成分が持つ抗酸化作用、コラーゲンの含有並びにペプチド機能性、コエンザイイムＱ１０の持つ抗酸化作用、細胞の活性化並びに老化防止作用、γ−トコフェノールの抗酸化作用並びに体内水分バランスの改善、さらにはＤＨＡ（ドコサヘキサエン酸）によるコレステロール及び中性脂肪の低減作用、抗酸化作用によって、総合的に代謝の活性化を促進し、老化防止、美容効果並びに体力亢進が期待できる栄養機能性材料を有効成分として含んでいる常備携帯に適した固形製剤の形の栄養機能性食品を提供することができる。 The nutritive functional food derived from Astragalus fish according to the present invention effectively contains the trophic function (component) derived from the Astragalus fish without losing the nutrient function (component), and as a result, the antioxidant functional ingredient derived from the Astragalus fish Action, collagen content and peptide functionality, antioxidant action of coenzyme Q10, cell activation and anti-aging action, antioxidant action of γ-tocophenol and improvement of water balance in the body, and DHA (docosahexaenoic acid) Permanent cell phone containing nutritional functional materials that can promote the activation of metabolism comprehensively by the action of reducing cholesterol and neutral fat by antioxidants and anti-oxidation, and that can be expected to prevent aging, enhance beauty and improve physical fitness. It is possible to provide a nutritional functional food in the form of a solid preparation suitable for the above.
実施例１
富山湾で捕獲されたカンテンゲンゲ洗浄し選別した後、約２ｃｍ大にカットし、カットした塊１ｋｇを回転刃を備えるミキサーへ移し、ミンチ状に破砕された試料を得た。このミンチ状の試料を約１００℃に沸騰させた水の中で煮沸した後、フリーズ・ドライすることによって乾燥させて塊状となったゲンゲ科魚類に由来する栄養機能性材料を得た。この塊状の組成物を粉砕して粉末状にした後、賦形剤、崩壊剤などを添加して混合し、公知の方法により打錠して５０ｍｇの本発明による栄養機能性材料からなる錠剤を作製した。
Example 1
After washing and sorting the Kantengage captured in Toyama Bay, it was cut into a size of about 2 cm, and 1 kg of the cut lump was transferred to a mixer equipped with a rotary blade to obtain a sample crushed into minced shapes. The minced sample was boiled in water boiled to about 100 ° C. and then freeze-dried to obtain a nutritional functional material derived from a genus genus fish that had been dried to be agglomerated. The pulverized composition is pulverized into a powder form, and then an excipient, a disintegrant and the like are added and mixed, and then tableted by a known method to give 50 mg of the tablet composed of the nutritional functional material according to the present invention. Produced.
実施例２
実施例１で得られたのと同じ粉末状の栄養機能性材料に賦形剤、崩壊剤などを添加して混合し、実施例１と同じ方法により打錠して１００ｍｇの本発明による栄養機能性材料からなる錠剤を作製した。
Example 2
100 mg of the nutritional function according to the present invention obtained by adding excipients, disintegrants and the like to the same powdery nutritional functional material obtained in Example 1 and mixing them, and tableting by the same method as in Example 1 A tablet made of a functional material was prepared.
実施例３
実施例１で得られたのと同じ粉末状の栄養機能性材料に下記の表１に示される成分を添加して混合し、さらに、この混合材料を公知の方法によりカプセルに充填して内容量３００ｍｇの本発明による栄養機能性材料を含むソフト・カプセルを作製した。
Example 3
Ingredients shown in Table 1 below are added to and mixed with the same pulverulent functional nutrient material obtained in Example 1, and the mixed material is filled into capsules by a known method, and the internal volume is increased. Soft capsules containing 300 mg of the nutritional functional material according to the present invention were made.
実施例４
実施例１で得られたのと同じ粉末状の栄養機能性材料に下記の表２に示される成分を添加して混合し、さらに、この混合材料を公知の方法によりカプセルに充填して内容量３３０ｍｇの本発明による栄養機能性材料を含むハード・カプセルを作製した。
Example 4
Ingredients shown in Table 2 below are added to and mixed with the same pulverulent functional functional material obtained in Example 1, and the mixed material is filled into capsules by a known method. Hard capsules containing 330 mg of the nutritional functional material according to the present invention were made.
試験例
実施例１〜４の錠剤型ゲンゲフリーズ・ドライ及びゲンゲフリーズ・ドライを配合したソフト・カプセル並びにハード・カプセルを試料として、電子スピン共鳴装置を用いて活性酸素消去活性を測定した。測定機器は、日本電子製ＪＥＯＬ、ＪＥＳ−ＦＲ３０型：電子スピン共鳴装置（ＥＳＲ）を用いた。本試験で使用した試料は、実施例１及び２のゲンゲフリーズ・ドライ（ゲンゲＦＤ）からなる錠剤：５０ｍｇ、１００ｍｇを各１個、実施例３のゲンゲフリーズ・ドライを配合したソフト・カプセルが１個のものと２個のもの、実施例４のゲンゲフリーズ・ドライを配合したハード・カプセルが１個のものと２個のもの用いて試験をした。前処理としては、０．１ＮＨＣＬ（ヒトの胃内のｐＨに相当し、ｐＨ１．５）、ｐＨ８．０の蒸留水（ヒトの小腸内のｐＨに相当）、ＭｅＯＨ、および７０％ＥｔＯＨの４種類の溶媒を用いて、試料注の抗酸化活性物質をそれぞれに抽出させた。得られた上澄液をサンプルとして、９６穴マイクロウエル中に、０．２ｍＭＦｅＳＯ４溶液：３７．５μＬ、Ｈ２Ｏ：３０μＬ、サンプル：３０μＬ、１０倍希釈液ＤＭＰＯ（５，５−Ｄｉｍｅｔｈｙ−１−ｐｙｒｒｏｌｉｎｅ−Ｎ−Ｏｘｉｄｅ、同仁化学研究所）：２０μＬ、１ｍＭＨ２Ｏ２：７５μＬを順次加えて混合し、反応液を得た。この反応液を室温で４５秒間反応させた後でＥＳＲ分析を行い、ヒドキシラジカルの消去率を測定した。試験結果を下記の表３に示す。
Test Example The active oxygen scavenging activity was measured using an electron spin resonance apparatus using soft capsules and hard capsules containing tablet-type Gengefreeze Dry and Gengefreeze Dry of Examples 1 to 4 as samples. JEOL, JES-FR30 type: electron spin resonance apparatus (ESR) manufactured by JEOL Ltd. was used as a measuring instrument. Samples used in this test were tablets made of Genge Freeze Dry (Genge FD) of Examples 1 and 2: 50 mg and 100 mg each, 1 soft capsule containing Genge Freeze Dry of Example 3 The test was performed using one and two hard capsules containing one and two hard capsules containing the Genge Freeze Dry of Example 4. As pre-treatment, 0.1 NHCL (corresponding to pH in human stomach, pH 1.5), distilled water of pH 8.0 (corresponding to pH in human small intestine), MeOH, and 70% EtOH Each of the antioxidant active substances in the sample injection was extracted using the above solvent. Using the obtained supernatant as a sample, in a 96-well microwell, 0.2 mM FeSO 4 solution: 37.5 μL, H 2 O: 30 μL, sample: 30 μL, 10-fold diluted solution DMPO (5,5-Dimethyl-1 -Pyrroline-N-Oxide, Dojindo Laboratories): 20 μL, 1 mM H 2 O 2 : 75 μL were sequentially added and mixed to obtain a reaction solution. The reaction solution was reacted at room temperature for 45 seconds and then subjected to ESR analysis to measure the rate of eradicating hydroxyl radicals. The test results are shown in Table 3 below.
表３に示す試験結果より、本発明によるゲンゲＦＤからなる錠剤及びゲンゲＦＤを含有したカプセルにおいて活性酸素消去活性が認められた。なお、実施例３のソフト・カプセルにおいては活性酸素消去活性が認められなかったが、これはカプセル化工程において使用する油脂が活性酸素消去活性を阻害したものと考えられる。
また、他の試料においては、いずれもＭｅＯＨや弱アルカリ水による抽出で高い活性酸素消去活性を示した。
From the test results shown in Table 3, active oxygen scavenging activity was observed in tablets made of Genge FD according to the present invention and capsules containing Genge FD. In the soft capsule of Example 3, no active oxygen scavenging activity was observed, but it is considered that the fat used in the encapsulation process inhibited the active oxygen scavenging activity.
In other samples, high active oxygen scavenging activity was shown by extraction with MeOH or weak alkaline water.
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| DE102010050570A1 (en) * | 2010-11-05 | 2012-05-10 | F. Holzer Gmbh | Composition and medicaments containing ω-3 fatty acids and an inhibitor of NF-κB transcription factor |
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