JP4372121B2 - Escherichia coli reducing agent in livestock intestines, fat reducing agent in livestock, and livestock breeding method - Google Patents

Escherichia coli reducing agent in livestock intestines, fat reducing agent in livestock, and livestock breeding method Download PDF

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JP4372121B2
JP4372121B2 JP2006174666A JP2006174666A JP4372121B2 JP 4372121 B2 JP4372121 B2 JP 4372121B2 JP 2006174666 A JP2006174666 A JP 2006174666A JP 2006174666 A JP2006174666 A JP 2006174666A JP 4372121 B2 JP4372121 B2 JP 4372121B2
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忠雄 上川
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本発明は、家畜腸内の大腸菌減少剤、家畜体内の脂肪減少剤及び家畜の飼育方法に関する。更に詳しくは、豚、鶏、牛、馬等といった家畜の腸内の大腸菌や家畜の体内の脂肪を減少させることができる家畜腸内の大腸菌減少剤、家畜体内の脂肪減少剤及び家畜の飼育方法に関する。 The present invention relates to an E. coli reducing agent in the intestines of livestock, a fat reducing agent in the livestock, and a method for raising livestock. More specifically, E. coli in the intestines of domestic animals such as pigs, chickens, cows, horses, etc. , an E. coli reducing agent in the intestines of livestock that can reduce fat in the livestock, a fat reducing agent in livestock, and a method for raising livestock About.

家畜を飼育する場合にあって、家畜用の飲料水としては地下水、河川水あるいは水道水などが用いられている。一方、地下水等をそのまま家畜に与えることは、地下水等に病原菌等が存在することもあり好ましくないので、地下水内の病原菌等を減少させる目的で、銅イオンや銀イオン等の制菌金属イオンを添加した家畜用飲料水が提供されている(例えば、特許文献1を参照。)。   In the case of raising livestock, groundwater, river water or tap water is used as drinking water for livestock. On the other hand, giving groundwater to livestock as it is is not preferable because pathogenic bacteria may exist in the groundwater, etc. For the purpose of reducing pathogenic bacteria in the groundwater, antibacterial metal ions such as copper ions and silver ions should be added. Added drinking water for livestock is provided (see, for example, Patent Document 1).

特開平11−56259号公報Japanese Patent Laid-Open No. 11-56259

ところで、近年にあっては、環境条件の変化等により、家畜もストレス等の理由で病気になるケースも多く、下痢になったり、体内の脂肪分が増加したりする等、健康な家畜を安定して生育させることが困難な状況にある。一方、従来の家畜用飲料水は、前記した特許文献1に報告されるように、地下水や河川水等といったいわば不衛生な水を飲料水として適用するために、地下水等に存在する病原菌等を滅菌する検討がなされることはあったものの、家畜の健康について飲料水により改善されたという検討は少なかった。このようなことから、家畜の健康について、毎日飲む飲料水によって改善することができれば好ましかった。   By the way, in recent years, due to changes in environmental conditions, livestock often become sick due to stress, etc., and stable livestock such as diarrhea and increased body fat content It is difficult to grow. On the other hand, as reported in Patent Document 1 described above, conventional livestock drinking water uses pathogenic bacteria or the like present in groundwater in order to apply so-called unsanitary water such as groundwater or river water as drinking water. Although studies on sterilization have been made, there have been few studies that the health of livestock has been improved by drinking water. For this reason, it was preferable if the health of livestock could be improved by drinking water every day.

本発明の目的は、前記の課題に鑑みてなされたものであり、豚、鶏、牛、馬等といった家畜の腸内の大腸菌や家畜体内の脂肪を減少させることができ、家畜の健康を改善・向上させることができる家畜腸内の大腸菌減少剤、家畜体内の脂肪減少剤及び家畜の飼育方法を提供することにある。 The object of the present invention has been made in view of the above-mentioned problems, and can reduce Escherichia coli in the intestines of livestock such as pigs, chickens, cows, horses, etc. and fat in the livestock, and improve the health of livestock. The object is to provide an Escherichia coli reducing agent in the intestines of livestock, a fat reducing agent in livestock, and a method of raising livestock that can be improved.

本発明の請求項1の家畜腸内の大腸菌減少剤は、家畜用の飲料水として使用され、次亜塩素酸の濃度が、残留塩素濃度として50〜200ppmであり、pHが6.0〜8.0である次亜塩素酸含有水からなることを特徴とする。 The E. coli reducing agent in the intestine of the first aspect of the present invention is used as drinking water for livestock, the concentration of hypochlorous acid is 50 to 200 ppm as the residual chlorine concentration, and the pH is 6.0 to 8 0.0. Hypochlorous acid-containing water which is 0.0.

本発明の請求項2の家畜体内の脂肪減少剤は、家畜用の飲料水として使用され、次亜塩素酸の濃度が、残留塩素濃度として50〜200ppmであり、pHが6.0〜8.0である次亜塩素酸含有水からなることを特徴とする。 The fat reducing agent in livestock of claim 2 of the present invention is used as drinking water for livestock, the concentration of hypochlorous acid is 50-200 ppm as the residual chlorine concentration, and the pH is 6.0-8. It consists of hypochlorous acid containing water which is zero.

本発明の請求項3の家畜の飼育方法は、請求項1に記載の家畜腸内の大腸菌減少剤を家畜に飲用させることを特徴とする。  A method for raising livestock according to claim 3 of the present invention is characterized in that the E. coli reducing agent in the intestine of livestock according to claim 1 is drunk to livestock.

本発明の請求項4の家畜の飼育方法は、前記請求項3において、前記家畜が豚、鶏、牛、馬、羊、山羊及びロバよりなる群より選ばれた少なくとも1種であることを特徴とする。  The method for raising livestock according to claim 4 of the present invention is characterized in that, in claim 3, the livestock is at least one selected from the group consisting of pigs, chickens, cows, horses, sheep, goats and donkeys. And

本発明の請求項5の家畜の飼育方法は、請求項2に記載の家畜体内の脂肪減少剤を家畜に飲用させることを特徴とする。  The domestic animal breeding method according to claim 5 of the present invention is characterized in that the fat reducing agent in the domestic animal body according to claim 2 is drunk to the domestic animal.

本発明の請求項6の家畜の飼育方法は、前記請求項5において、前記家畜が豚、鶏、牛、馬、羊、山羊及びロバよりなる群より選ばれた少なくとも1種であることを特徴とする。  The method for raising livestock according to claim 6 of the present invention is characterized in that, in claim 5, the livestock is at least one selected from the group consisting of pigs, chickens, cows, horses, sheep, goats and donkeys. And

本発明の請求項1の家畜腸内の大腸菌減少剤は、次亜塩素酸の濃度が、残留塩素濃度として50〜200ppmである次亜塩素酸含有水からなるので、通常の家畜用飲料水として使用することができるほか、家畜腸内の大腸菌を減少させ、家畜腸内の乳酸菌数には影響を与えず大腸菌数のみを有意に減少させることを可能とし、家畜の健康を改善・向上することができる家畜腸内の大腸菌減少剤となる。
また、本発明の請求項1の家畜腸内の大腸菌減少剤は、pHの範囲を特定の範囲としているので、飲料水中に次亜塩素酸が好適に残存し、前記した本発明の効果を効率よく発揮することができるとともに、家畜が飲むにも抵抗がない家畜腸内の大腸菌減少剤を提供することができる。
Since the Escherichia coli reducing agent in the intestinal gut of claim 1 of the present invention is composed of hypochlorous acid-containing water having a hypochlorous acid concentration of 50 to 200 ppm as a residual chlorine concentration, In addition to reducing the number of coliforms in the intestines of livestock and making it possible to significantly reduce only the number of coliforms without affecting the number of lactic acid bacteria in the intestines of livestock, It becomes an E. coli reducing agent in the intestines of livestock .
Moreover, since the Escherichia coli reducing agent in the intestines of livestock according to claim 1 of the present invention has a specific pH range, hypochlorous acid suitably remains in the drinking water, and the above-described effects of the present invention can be efficiently obtained. It is possible to provide an E. coli reducing agent in the intestines of livestock that can be exerted well and the livestock is not resistant to drinking.

本発明の請求項2の家畜体内の脂肪減少剤は、次亜塩素酸の濃度が、残留塩素濃度として50〜200ppmである次亜塩素酸含有水からなるので、通常の家畜用飲料水として使用することができるほか、家畜体内の脂肪を減少させ、家畜体内の脂肪分(体脂肪、内臓脂肪等)を筋肉分等に置換させ、家畜の体重を減少させることなく家畜体内の脂肪分を減少させることができ、家畜の健康を改善・向上することができる家畜体内の脂肪減少剤となる。  The fat reducing agent in the livestock body of claim 2 of the present invention is composed of hypochlorous acid-containing water having a hypochlorous acid concentration of 50 to 200 ppm as a residual chlorine concentration. In addition to reducing fat in the livestock, replacing fat in the livestock (body fat, visceral fat, etc.) with muscle, etc., reducing fat in the livestock without reducing the weight of the livestock It becomes a fat reducing agent in the livestock body that can be improved and can improve the health of livestock.
また、本発明の請求項2の家畜体内の脂肪減少剤は、pHの範囲を特定の範囲としているので、飲料水中に次亜塩素酸が好適に残存し、前記した本発明の効果を効率よく発揮することができるとともに、家畜が飲むにも抵抗がない家畜体内の脂肪減少剤を提供することができる。  Moreover, since the fat reducing agent in the livestock body of Claim 2 of this invention makes the range of pH into a specific range, hypochlorous acid remains suitably in drinking water, The above-mentioned effect of this invention is made efficient. It is possible to provide a fat reducing agent in livestock that can be exerted and that is not resistant to drinking by livestock.

本発明の請求項3の家畜の飼育方法は、前記した本発明の家畜腸内の大腸菌減少剤を家畜に飲用させて家畜を飼育することにより、家畜に飲料水を与えるだけで家畜腸内の大腸菌を減少させ、家畜の健康を維持・向上させることが可能となる家畜の飼育方法を提供する。  The method for raising livestock according to claim 3 of the present invention is such that the aforementioned E. coli reducing agent in the intestinal gut of the present invention is drunk to the livestock and the livestock is bred, so that only the drinking water is given to the livestock. Provided is a livestock breeding method capable of reducing E. coli and maintaining and improving livestock health.

本発明の請求項4の家畜の飼育方法は、前記した家畜が豚、鶏、牛、馬、羊、山羊及びロバよりなる群より選ばれた少なくとも1種であるので、これらの家畜の健康を改善・向上させることが可能となる家畜の飼育方法を好適に提供することができる。  In the method for raising livestock according to claim 4 of the present invention, the livestock is at least one selected from the group consisting of pigs, chickens, cows, horses, sheep, goats and donkeys. A method for raising livestock that can be improved and improved can be suitably provided.

本発明の請求項5の家畜の飼育方法は、前記した本発明の家畜体内の脂肪減少剤を家畜に飲用させて家畜を飼育することにより、家畜に飲料水を与えるだけで家畜体内の脂肪を減少させ、家畜の健康を維持・向上させることが可能となる家畜の飼育方法を提供する。  The domestic animal breeding method according to claim 5 of the present invention is that the fat reducing agent in the livestock body of the present invention is drunk to the livestock, and the livestock is reared so that the fat in the livestock body can be obtained simply by providing the livestock with drinking water. Provided is a method of raising livestock that can reduce and maintain and improve the health of livestock.

本発明の請求項6の家畜の飼育方法は、前記した家畜が豚、鶏、牛、馬、羊、山羊及びロバよりなる群より選ばれた少なくとも1種であるので、これらの家畜の健康を改善・向上させることが可能となる家畜の飼育方法を好適に提供することができる。  In the method for raising livestock according to claim 6 of the present invention, the above-mentioned livestock is at least one selected from the group consisting of pigs, chickens, cows, horses, sheep, goats and donkeys. A method for raising livestock that can be improved and improved can be suitably provided.

本発明の家畜腸内の大腸菌減少剤または家畜体内の脂肪減少剤は、次亜塩素酸の濃度が、残留塩素濃度として50〜200ppmである次亜塩素酸含有水からなるものであり、主として豚、鶏、牛、馬、羊、山羊、ロバ等の哺乳類、鳥類等の家畜用の飲料水として使用される。 The Escherichia coli reducing agent in livestock intestines or the fat reducing agent in livestock of the present invention is composed of hypochlorous acid-containing water having a hypochlorous acid concentration of 50 to 200 ppm as a residual chlorine concentration. It is used as drinking water for mammals such as chickens, cows, horses, sheep, goats and donkeys, and livestock such as birds.

本発明の家畜腸内の大腸菌減少剤または家畜体内の脂肪減少剤を構成する次亜塩素酸含有水は、例えば、水に対して次亜塩素酸ナトリウム(NaClO)と、塩酸(HCl)等の酸を添加する方法を用いることにより調製することができる。 Hypochlorous acid-containing water constituting the Escherichia coli reducing agent in livestock intestines or the fat reducing agent in livestock of the present invention is, for example, sodium hypochlorite (NaClO), hydrochloric acid (HCl), etc. It can be prepared by using a method of adding an acid.

次亜塩素酸含有水の調製にあっては、水道水、地下水、河川水等の水に対して、次亜塩素酸ナトリウムと塩酸(あるいは塩酸水溶液)を一緒に添加せずに、次亜塩素酸ナトリウムを添加し、均一に混合した後、塩酸(あるいは塩酸水溶液)を添加し、再度均一に混合することにより、次亜塩素酸ナトリウムと塩酸の反応による塩素ガスの発生を極力抑えることができる。また、これとは逆に、水に対して塩酸(あるいは塩酸水溶液)を添加し、均一に混合した後、次亜塩素酸ナトリウムを添加し、再度均一に混合するようにしてもよい。なお、酸としては、前記した塩酸のほかの公知の酸を使用することができるが、飲料水に適用することに鑑みれば、塩酸を使用することが好ましい。   In the preparation of hypochlorous acid-containing water, hypochlorous acid is added to tap water, ground water, river water, etc. without adding sodium hypochlorite and hydrochloric acid (or aqueous hydrochloric acid) together. Sodium chloride is added and mixed uniformly, then hydrochloric acid (or aqueous hydrochloric acid solution) is added and mixed uniformly again, so that generation of chlorine gas due to the reaction between sodium hypochlorite and hydrochloric acid can be suppressed as much as possible. . Conversely, hydrochloric acid (or aqueous hydrochloric acid solution) may be added to water and mixed uniformly, and then sodium hypochlorite may be added and mixed uniformly again. In addition, as an acid, although well-known acid other than the above-mentioned hydrochloric acid can be used, it is preferable to use hydrochloric acid in view of applying to drinking water.

また、次亜塩素酸含有水を調製するには、上記の方法以外を用いてもよく、例えば、塩化ナトリウム(NaCl)を有隔膜電界槽で電気分解して、陽極側から得られる次亜塩素酸を水溶液としてもよく、また、塩酸を無隔膜電界槽で電気分解して、陰極側から得られる次亜塩素酸を水溶液としてもよい。また、これら以外の方法で調製するようにしてもよい。   In addition, in order to prepare hypochlorous acid-containing water, methods other than those described above may be used. For example, hypochlorous acid obtained from the anode side by electrolyzing sodium chloride (NaCl) in a diaphragm electric field tank An acid may be used as an aqueous solution, or hypochlorous acid obtained from the cathode side by electrolysis of hydrochloric acid in a non-diaphragm electric field bath may be used as an aqueous solution. Moreover, you may make it prepare by methods other than these.

本発明の家畜腸内の大腸菌減少剤または家畜体内の脂肪減少剤における次亜塩素酸の濃度は、残留塩素濃度として50〜200ppmである。次亜塩素酸の濃度がかかる範囲であれば、家畜腸内の大腸菌減少効果を備えた家畜腸内の大腸菌減少剤及び家畜体内の脂肪減少効果を備えた家畜体内の脂肪減少剤を家畜も通常の飲料水と区別なく飲むことができる。一方、残留塩素濃度が50ppmより低いと、効果が現れにくい場合があり、残留塩素濃度が200ppmより高いと、飲料水として飲みにくい等の問題がある。家畜腸内の大腸菌減少剤または家畜体内の脂肪減少剤における次亜塩素酸の濃度は、残留塩素濃度として100〜200ppmであることが好ましい。 The concentration of hypochlorous acid in the Escherichia coli reducing agent in livestock intestines or the fat reducing agent in livestock of the present invention is 50 to 200 ppm as the residual chlorine concentration. If the above range the concentration of hypochlorous acid, Escherichia coli reducing agents in livestock gut with E. coli reducing effect in livestock gut, and livestock fat reducing agent in livestock body having a fat reducing effect in livestock body You can drink without distinction from normal drinking water. On the other hand, if the residual chlorine concentration is lower than 50 ppm, the effect may be difficult to appear, and if the residual chlorine concentration is higher than 200 ppm, there is a problem that it is difficult to drink as drinking water. The concentration of hypochlorous acid in the E. coli reducing agent in the livestock intestines or the fat reducing agent in the livestock body is preferably 100 to 200 ppm as the residual chlorine concentration.

また、含有水中の次亜塩素酸は、当該含有水のpHに依存して、その存在状態が異なることが知られており、pHが高くなるにつれて、塩素ガス(Cl)、次亜塩素酸(HClO)、次亜塩素酸イオン(ClO)と存在状態が変化する。 Further, hypochlorous acid in the contained water is known to have different existence states depending on the pH of the contained water. As the pH increases, chlorine gas (Cl 2 ), hypochlorous acid (HClO), hypochlorite ion (ClO ) and the state of existence change.

図1は、次亜塩素酸含有水のpHと次亜塩素酸の存在率(残存割合)との関係を示した図である。図1からわかるように、含有水中のpHが1.0程度の場合には約35%が次亜塩素酸、約65%が塩素ガスであり、pHが大きくなるにつれて次亜塩素酸の存在率が高くなり、pHが2.0程度では約70%、pHが3.0程度ではその90%以上となり、塩素ガスの形で存在するのは残りの分だけになる。また、pHが4.0程度ではほぼ100%が次亜塩素酸の形で存在し、さらに、pHが8.0程度では10%弱が次亜塩素酸となり、残りの90%強は次亜塩素酸イオン(ClO)となる。 FIG. 1 is a graph showing the relationship between the pH of hypochlorous acid-containing water and the abundance (remaining ratio) of hypochlorous acid. As can be seen from FIG. 1, when the pH of the contained water is about 1.0, about 35% is hypochlorous acid and about 65% is chlorine gas, and the abundance of hypochlorous acid as the pH increases. When the pH is about 2.0, it is about 70%, and when the pH is about 3.0, it is 90% or more. Only the remaining amount exists in the form of chlorine gas. Further, when the pH is about 4.0, almost 100% is present in the form of hypochlorous acid, and when the pH is about 8.0, slightly less than 10% is hypochlorous acid, and the remaining 90% is hypochlorous acid. It becomes a chlorate ion (ClO ).

本発明にあっては、殺菌力等の高い次亜塩素酸がある程度残存するpHが8.0以下とすればよいが、家畜用の飲料水として使用することを考えれば、一般に、pHが5.0より小さいと、酸性度が強く、飲みにくくなる場合もある。かかる場合を考慮して、次亜塩素酸含有水のpHは、6.0〜8.0とすればよく、本発明の家畜腸内の大腸菌減少剤または家畜体内の脂肪減少剤のpHをかかる範囲とすることにより、次亜塩素酸が高い割合で存在するため本発明の効果を効率よく発揮することができ、かつ、家畜が飲むにも抵抗がない家畜腸内の大腸菌減少剤または家畜体内の脂肪減少剤を提供することができる。なお、家畜腸内の大腸菌減少剤または家畜体内の脂肪減少剤のpHは、6.0〜7.0であることが特に好ましい。 In the present invention, the pH at which some hypochlorous acid having high sterilizing power remains to some extent may be set to 8.0 or less. However, in consideration of use as drinking water for livestock, generally, the pH is 5 If it is less than 0.0, the acidity is strong and it may be difficult to drink. In consideration of such a case, the pH of the hypochlorous acid-containing water may be a 6.0 to 8.0, such a pH of E. coli reducing agent or livestock body fat reducing agent in the livestock gut of this invention By setting the range, hypochlorous acid is present at a high rate, so that the effects of the present invention can be efficiently exhibited, and the E. coli reducing agent in the livestock intestine that is not resistant to drinking by livestock or the livestock body. The fat reducing agent can be provided. In addition, it is particularly preferable that the pH of the Escherichia coli reducing agent in the livestock intestines or the fat reducing agent in the livestock body is 6.0 to 7.0.

なお、本発明の家畜腸内の大腸菌減少剤または家畜体内の脂肪減少剤には、本発明の効果を妨げない範囲内で、例えば、無機塩類、アルカリ金属、アルカリ土類金属などの各種栄養源及び抗生物質その他の薬剤等の添加剤を適宜添加することができる。また、ベースの水となる地下水等に病原菌等が存在することを考慮して、公知の滅菌処理を施すようにしてもよい。 It should be noted that the E. coli reducing agent in the intestine of the present invention or the fat reducing agent in the livestock within the range that does not interfere with the effects of the present invention, for example, various nutrient sources such as inorganic salts, alkali metals, alkaline earth metals, etc. In addition, additives such as antibiotics and other drugs can be added as appropriate. In addition, a known sterilization process may be performed in consideration of the presence of pathogenic bacteria or the like in the ground water or the like serving as the base water.

前記した構成からなる本発明の家畜腸内の大腸菌減少剤または家畜体内の脂肪減少剤は、家畜を飼育する際の飲料水として用いることができることはもちろんであるが、当該家畜腸内の大腸菌減少剤または家畜体内の脂肪減少剤を家畜に飲用させて家畜の飼育方法に用いることにより、下記のような効果を奏し、家畜の健康を改善・向上することができる。 Of course, the Escherichia coli reducing agent in the livestock intestine or the fat reducing agent in the livestock body of the present invention having the above-described configuration can be used as drinking water when raising livestock. By using an agent or a fat reducing agent in the livestock for a domestic animal breeding method, the following effects can be obtained and the health of the livestock can be improved / improved.

例えば、本発明の家畜腸内の大腸菌減少剤を豚、鶏、牛、馬、羊、山羊、ロバ等の哺乳類、鳥類等の家畜に飲用させてこれらの家畜を飼育することにより、これらの家畜の腸内の大腸菌を減少させることができる。本発明にあっては、腸内の大腸菌を有意に減少させることができる一方、腸内の総菌と乳酸菌の数には影響を及ぼさず、腸内の大腸菌のみを減少させることができるものである。 For example, the livestock of these livestock can be raised by allowing the Escherichia coli reducing agent in the livestock intestines of the present invention to be drunk to mammals such as pigs, chickens, cows, horses, sheep, goats and donkeys, and domestic animals such as birds. E. coli in the gut can be reduced. In the present invention, E. coli in the intestine can be significantly reduced, while the number of total bacteria and lactic acid bacteria in the intestine is not affected, and only E. coli in the intestine can be reduced. is there.

すなわち、家畜においては、Lactobacillusに代表される乳酸菌はいわゆる「善玉菌」として存在し、サルモネラなどの病原菌の腸管定着阻止、飼料利用率の増加、発育の増加などの効果を果たしているため、腸内で減少させることは好ましくない。一方、大腸菌はいわゆる「悪玉菌」であり、病原性大腸菌感染による大腸菌症のみならず、各種ストレス条件下では小腸内で増殖し下痢を引き起こすことが知られている。本発明の家畜腸内の大腸菌減少剤を飲用することにより、家畜腸内の乳酸菌数には影響を与えず大腸菌数のみを有意に減少させることができるので、家畜の腸内フロ−ラの維持に肯定的な役割を果たすことができる That is, in livestock, the lactic acid bacteria represented by Lactobacillus exist as so-called “good bacteria” and have effects such as prevention of intestinal colonization of pathogenic bacteria such as Salmonella, increase in feed utilization, and increase in growth. It is not preferable to reduce the amount by. On the other hand, Escherichia coli is a so-called “bad bacilli” and is known to grow in the small intestine and cause diarrhea under various stress conditions as well as Escherichia coli caused by pathogenic E. coli infection. By drinking the E. coli reducing agent in the intestine of the livestock of the present invention, it is possible to significantly reduce only the number of E. coli without affecting the number of lactic acid bacteria in the intestine of the livestock. Can play a positive role .

また、本発明の家畜体内の脂肪減少剤を豚、鶏、牛、馬、羊、山羊、ロバ等の哺乳類、鳥類等の家畜に飲用させてこれらの家畜を飼育することにより、家畜の体内の脂肪を減少させることができる。このように、本発明の家畜体内の脂肪減少剤は、家畜の体内の脂肪分(体脂肪、内臓脂肪等)を好適に減少させることができるが、本発明にあっては、家畜体内の脂肪分を筋肉分等に置換させるため、家畜の体重を減少させることなく家畜体内の脂肪分を減少させることが可能となる。 In addition, the fat reducing agent in the livestock body of the present invention can be drunk to mammals such as pigs, chickens, cows, horses, sheep, goats, donkeys, etc. Can reduce fat. As described above, the fat reducing agent in the livestock body of the present invention can suitably reduce the fat content (body fat, visceral fat, etc.) in the livestock body. in order to replace the partial muscle amount and the like, that Do is possible to reduce the fat content of livestock body without reducing the body weight of domestic animals.

家畜の飼育に際しては、水道水等、家畜に通常飲用させる飲料水を本発明の家畜腸内の大腸菌減少剤または家畜体内の脂肪減少剤に代替して飲用させるようにすればよいが、通常与える量の50〜100%程度を家畜に飲用させるようにすればよく、80〜100%とすれば更に効果的であり、略100%とすれば特に効果的である。 When raising livestock, drinking water that is normally consumed by livestock, such as tap water, may be used instead of the Escherichia coli reducing agent in the intestines of the livestock or fat reducing agent in the livestock body of the present invention. About 50 to 100% of the amount may be drunk to livestock, 80 to 100% is more effective, and about 100% is particularly effective.

以下、実施例に基づき本発明をさらに詳細に説明するが、本発明は、かかる実施例の内容に限定されるものではない。   EXAMPLES Hereinafter, although this invention is demonstrated further in detail based on an Example, this invention is not limited to the content of this Example.

[実施例1]
鶏の腸内の大腸菌減少効果の確認:
供試動物として、生後145日齢のハイラインマリアの雌の鶏を用い、下記の方法により、腸内の大腸菌減少効果を確認した。
[Example 1]
Confirmation of E. coli reduction in chicken intestines:
As a test animal, a 145-day-old high-line Maria female chicken was used, and the intestinal E. coli reduction effect was confirmed by the following method.

(1)次亜塩素酸含有水の製造:
水道水に対して、12%食品添加物次亜塩素酸ナトリウム水溶液を添加し、均一に混合した後、8.5%の食品添加物塩酸水溶液を添加して、pHが6.0〜7.0、残留塩素濃度が150〜200ppmの次亜塩素酸含有水を調製して、本発明の家畜腸内の大腸菌減少剤とした。
(1) Production of hypochlorous acid-containing water:
12% food additive sodium hypochlorite aqueous solution is added to tap water and mixed uniformly, then 8.5% food additive hydrochloric acid aqueous solution is added, and the pH is 6.0 to 7. Hypochlorous acid-containing water having a residual chlorine concentration of 150 to 200 ppm was prepared as the Escherichia coli reducing agent in the intestines of livestock of the present invention.

(2)腸内細菌の確認:
鶏の腸内細菌として、Escherichia coli(大腸菌)数のほか、総菌数、乳酸菌数を測定した。供試動物として、生後145日齢のハイラインマリアの雌の鶏を20羽用意して、うち10羽を本発明の家畜腸内の大腸菌減少剤の飲水投与群(被検群)、残り10羽を対照として水道水の飲水投与群(対照群)とした。被検群には被検水を、対照群には水道水を自由飲水の形で給与して鶏を飼育するようにして、合計8週間評価を行った。
(2) Confirmation of enteric bacteria:
In addition to the number of Escherichia coli (E. coli), the total number of bacteria and the number of lactic acid bacteria were measured as enteric bacteria of chickens. As test animals, 20 145 day-old high-line Maria female chickens were prepared, of which 10 were the drinking water administration group (test group) of the Escherichia coli reducing agent in the livestock intestine of the present invention, and the remaining 10 A drinking water administration group (control group) was used with wings as a control. The test group was fed test water, and the control group was fed tap water in the form of free drinking, and the chickens were raised for evaluation for a total of 8 weeks.

菌数の確認については、試験開始後3週目から毎週1回ずつ、6週間に渡り(8週目まで)新鮮糞便を採取し、腸内細菌の検索を行った。具体的には、鶏の新鮮糞便約1gをできる限り無菌的に採取し、供試検体とした。供試検体は採取後ただちに嫌気性希釈液に混和し、さらに希釈液で10倍階段で希釈し適当な濃度としたものを、LBS寒天培地(BBL社製)、5%馬脱繊血加BL寒天培地(栄研社製)、DHL寒天培地(栄研社製)に塗沫し、DHL寒天培地は好気条件下で、LBS寒天培地、5%馬脱繊血加BL寒天培地は嫌気条件下で、37℃、24〜48時間培養した。   For confirmation of the number of bacteria, fresh feces were collected once every week from the 3rd week after the start of the test for 6 weeks (up to the 8th week) to search for enteric bacteria. Specifically, about 1 g of fresh stool from chicken was aseptically collected as possible and used as a test sample. Immediately after collection, the test sample was mixed with an anaerobic diluent, and diluted to 10-fold with the diluent to a suitable concentration. LBS agar medium (manufactured by BBL), 5% horse defibrillated blood-added BL Agar medium (Eiken Co., Ltd.) and DHL agar medium (Eiken Co., Ltd.) are smeared, DHL agar medium is under aerobic condition, LBS agar medium, 5% horse defibrillated BL agar medium is anaerobic condition The cells were cultured at 37 ° C. for 24-48 hours.

総菌数については5%馬脱繊血加BL寒天培地に発育してきたコロニ−数を測定した。また、乳酸菌数についてはLBS寒天培地及び5%馬脱繊血加BL寒天培地に、大腸菌数についてはDHL寒天培地に発育してきたコロニ−について、それぞれの培地で典型的なコロニ−性状を示す菌株について菌数を測定後、釣菌・純培養した。   Regarding the total number of bacteria, the number of colonies that had grown on 5% equine defibrinated blood-containing BL agar medium was measured. Moreover, about the number of lactic acid bacteria, it is a LBS agar medium and 5% horse defiltrated BL agar medium, and about the number of colon_bacillus | E._coli, the colony which showed typical colony property in each culture medium After measuring the number of bacteria, the fish were purely cultured.

そして、分離菌について、グラム染色、好気または嫌気条件下での発育性、生化学的性状などを調べ、Barrow & Felthamの分類にしたがって、属のレベルで同定した。結果を表1〜表3に示す。なお、結果は平均値(n=10)である。   The isolates were then examined for Gram staining, growth under aerobic or anaerobic conditions, biochemical properties, etc., and identified at the genus level according to the Barrow & Feltham classification. The results are shown in Tables 1 to 3. The result is an average value (n = 10).

(結果:大腸菌数)

Figure 0004372121
(Result: E. coli count)
Figure 0004372121

(結果:総菌数)

Figure 0004372121
(Result: total bacterial count)
Figure 0004372121

(結果:乳酸菌数)

Figure 0004372121
(Result: Number of lactic acid bacteria)
Figure 0004372121

表1に示すように、大腸菌数(大腸菌群数:logCFU/g)は、本発明の家畜腸内の大腸菌減少剤を飲用した被検群では3.1〜4.7、水道水を飲用した対照群では4.6〜5.7で、被検群では時間の経過とともに菌数が減少する傾向がみられ、被検水について投与後4〜8週目では対照群に比べて有意に菌数が少なかった。 As shown in Table 1, the number of E. coli (E. coli group number: log CFU / g) was 3.1 to 4.7 in the test group drinking the Escherichia coli reducing agent in the intestines of the present invention, and tap water was drunk. In the control group, the number of bacteria tends to decrease with the passage of time in the test group, and in the test water, the bacteria were significantly compared to the control group at 4 to 8 weeks after administration. The number was small.

表2に示すように、総菌数(総菌群数:logCFU/g)は被検群と対照群のそれぞれの平均が、被検群では8.1〜8.7、対照群では8.1〜8.9で、両群間にはいずれの調査週においても菌数に有意な差は認められなかった。   As shown in Table 2, the total number of bacteria (total number of bacterial groups: log CFU / g) is the average of the test group and the control group, 8.1 to 8.7 in the test group, and 8. in the control group. From 1 to 8.9, there was no significant difference in the number of bacteria between the two groups in any study week.

表3に示すように、乳酸菌数(乳酸菌群数:logCFU/g)は、被検群では8.0〜8.5、対照群では8.1〜8.5で、両群間でいずれの調査週においても菌数に有意な差は認められなかった。なお、分離された乳酸菌についてはいずれもLactobacillusであった。   As shown in Table 3, the number of lactic acid bacteria (number of lactic acid bacteria group: log CFU / g) was 8.0 to 8.5 in the test group, 8.1 to 8.5 in the control group, There was no significant difference in the number of bacteria even during the study week. The isolated lactic acid bacteria were all Lactobacillus.

以上の結果から分かるように、鶏に次亜塩素酸含有水を経口投与した場合、水道水を投与した場合と比べ、糞便中の総菌数と乳酸菌数には変化が認められなかったが、大腸菌数は試験開始後4週目からは有意に減少することが確認できた。被検水の投与は鶏腸管内の乳酸菌数には影響を与えない一方、大腸菌数は有意に減したことから、鶏の腸内フロ−ラの維持には肯定的な役割を果たしている可能性が示唆され、家畜腸内の大腸菌減少剤として適用することができることが確認できた。 As can be seen from the above results, when hypochlorous acid-containing water was orally administered to chickens, no change was observed in the total number of bacteria in stool and the number of lactic acid bacteria compared to the case where tap water was administered. It was confirmed that the number of E. coli decreased significantly from the 4th week after the start of the test. The administration of the test water did not affect the number of lactic acid bacteria in the chicken intestine, but the number of E. coli was significantly reduced, which may play a positive role in maintaining the chicken intestinal flora. There is suggested, it was confirmed that can be applied as E. coli-reducing agent in the house畜腸.

[実施例2]
肉豚の体内の脂肪減少効果の確認:
供試動物として肉用の豚を用い、下記の方法により、肉になった場合の脂肪減少効果を確認した。
[Example 2]
Confirmation of fat reduction effect in beef pork body:
Meat pork was used as a test animal, and the fat reduction effect when it became meat was confirmed by the following method.

実施例1(1)で調製した次亜塩素酸含有水を用い、また、供試動物として、ランドレース種、大ヨークシャー種、デュロック種を交配した肉豚を32頭用意して、うち16頭を本発明の家畜体内の脂肪減少剤の飲水投与群(被検群)、残り16頭を対照として水道水の飲水投与群(対照群)とした。そして、被検群には被検水を、対照群には水道水を供給し、それぞれ飲用させるようにして肉豚を飼育するようにした。試験期間は3月とした。3月経過後の被検群のロース部分における切り出し肉の写真の代表例を図2に、対照群のロース部分における切り出し肉の写真の代表例を図3にそれぞれ示す。 Using the hypochlorous acid-containing water prepared in Example 1 (1), and preparing 32 breed pigs mated with Landrace, Large Yorkshire, and Duroc as test animals, 16 of them Was used as a drinking water administration group (test group) of fat reducing agents in livestock of the present invention, and the remaining 16 animals were used as drinking water administration groups (control group). Then, test water was supplied to the test group, and tap water was supplied to the control group. The test period was March. A representative example of a photograph of the cut meat in the loin portion of the test group after the elapse of three months is shown in FIG. 2, and a representative example of a photograph of the cut meat in the loin portion of the control group is shown in FIG.

なお、肉豚に対しての家畜体内の脂肪減少剤及び水道水の供給は、豚房(飼育している豚の宿舎)の傍に容量500リットルのタンクを設置し、ポンプ及び、豚房まで配管した塩ビ管を通して、肉豚へ供給するようにした。豚房への供給は、1週間に2回の割合にてタンクへ追加するようにした。なお、肉豚は、豚房に配設された専用の蛇口から肉豚が直接家畜体内の脂肪減少剤または水道水を飲むものである。 In addition, the supply of fat reducing agent and tap water in livestock to beef pigs is set up by a 500 liter tank beside the pig bun (house of pigs being raised), to the pump and the pig bun It was made to supply to the pork through the piped PVC pipe. The supply to the pig bun was added to the tank twice a week. In addition, beef pigs are those in which the pork drinks the fat reducing agent or tap water in the livestock directly from a dedicated faucet provided in the pig bun.

図2及び図3の結果から分かるように、対照群と比較して被検群の脂肪分(脂身)は少なかった。また、図2に現される被検群にあっては、肉豚体内の脂肪分は筋肉分等に置換されたものと考えられる。この結果より、本発明の家畜体内の脂肪減少剤を飲用することにより、家畜体内の脂肪減少を行うことができることが確認できた。 As can be seen from the results of FIGS. 2 and 3, the test group had less fat (fat) than the control group. Further, in the test group shown in FIG. 2, it is considered that the fat content in the body of the pork has been replaced with the muscle content. From these results, it was confirmed that the fat in the livestock can be reduced by drinking the fat reducing agent in the livestock of the present invention.

本発明は、例えば、豚、鶏、牛、馬等の家畜を健康的に飼育するための家畜腸内の大腸菌減少剤、家畜体内の脂肪減少剤及び家畜の飼育方法として使用することができる。 INDUSTRIAL APPLICABILITY The present invention can be used, for example, as an Escherichia coli reducing agent in the livestock intestines, a fat reducing agent in livestock, and a domestic animal breeding method for healthy breeding of livestock such as pigs, chickens, cows and horses.

次亜塩素酸含有水のpHと次亜塩素酸の存在率との関係を示した図である。It is the figure which showed the relationship between the pH of hypochlorous acid containing water, and the abundance rate of hypochlorous acid. 被検群のロース部分における切り出し肉の写真の代表例を示した図である。It is the figure which showed the representative example of the photograph of the cut meat in the loin part of a test group. 対照群のロース部分における切り出し肉の写真の代表例を示した図である。It is the figure which showed the representative example of the photograph of the cut meat in the loin part of a control group.

Claims (6)

家畜用の飲料水として使用され、次亜塩素酸の濃度が、残留塩素濃度として50〜200ppmであり、pHが6.0〜8.0である次亜塩素酸含有水からなることを特徴とする家畜腸内の大腸菌減少剤。  It is used as drinking water for livestock and is characterized by comprising hypochlorous acid-containing water having a concentration of hypochlorous acid of 50 to 200 ppm as a residual chlorine concentration and a pH of 6.0 to 8.0. E. coli reducing agent in livestock intestines. 家畜用の飲料水として使用され、次亜塩素酸の濃度が、残留塩素濃度として50〜200ppmであり、pHが6.0〜8.0である次亜塩素酸含有水からなることを特徴とする家畜体内の脂肪減少剤。  It is used as drinking water for livestock and is characterized by comprising hypochlorous acid-containing water having a concentration of hypochlorous acid of 50 to 200 ppm as a residual chlorine concentration and a pH of 6.0 to 8.0. A fat reducing agent in livestock. 請求項1に記載の家畜腸内の大腸菌減少剤を家畜に飲用させることを特徴とする家畜の飼育方法。A method for raising livestock, comprising causing livestock to drink the Escherichia coli reducing agent in intestines of claim 1. 前記家畜が豚、鶏、牛、馬、羊、山羊及びロバよりなる群より選ばれた少なくとも1種であることを特徴とする請求項3に記載の家畜の飼育方法。The method of raising livestock according to claim 3, wherein the livestock is at least one selected from the group consisting of pigs, chickens, cows, horses, sheep, goats and donkeys. 請求項2に記載の家畜体内の脂肪減少剤を家畜に飲用させることを特徴とする家畜の飼育方法。A domestic animal breeding method, wherein the domestic animal fat reducing agent according to claim 2 is drunk. 前記家畜が豚、鶏、牛、馬、羊、山羊及びロバよりなる群より選ばれた少なくとも1種であることを特徴とする請求項5に記載の家畜の飼育方法。6. The method for raising livestock according to claim 5, wherein the livestock is at least one selected from the group consisting of pigs, chickens, cows, horses, sheep, goats and donkeys.
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KR20220014487A (en) * 2020-07-29 2022-02-07 연세대학교 원주산학협력단 Drinking water composition for hypochlorous acid livestock

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US10617716B2 (en) * 2014-12-16 2020-04-14 Urgo Us, Inc. Hypochlorous acid formulations and methods for treating skin conditions
CN106901055A (en) * 2017-03-14 2017-06-30 安徽标王农牧有限公司 A kind of 130 to 500 days Huaibei partridge chicken laying periods compound complete feed
CN106942510A (en) * 2017-03-14 2017-07-14 安徽标王农牧有限公司 A kind of 35 to 130 days compound complete feeds of Huaibei partridge chicken finishing period

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KR20220014487A (en) * 2020-07-29 2022-02-07 연세대학교 원주산학협력단 Drinking water composition for hypochlorous acid livestock
KR102461469B1 (en) * 2020-07-29 2022-10-31 연세대학교 원주산학협력단 Drinking water composition for hypochlorous acid livestock

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