JP4180902B2 - A constant temperature / humidity device that can keep the observation object in the culture vessel placed on the microscope alive for a long time. - Google Patents

A constant temperature / humidity device that can keep the observation object in the culture vessel placed on the microscope alive for a long time. Download PDF

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JP4180902B2
JP4180902B2 JP2002364234A JP2002364234A JP4180902B2 JP 4180902 B2 JP4180902 B2 JP 4180902B2 JP 2002364234 A JP2002364234 A JP 2002364234A JP 2002364234 A JP2002364234 A JP 2002364234A JP 4180902 B2 JP4180902 B2 JP 4180902B2
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microscope
humidity
temperature
constant temperature
culture vessel
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JP2004198538A (en
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いずみ 小川
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小川 明生
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers
    • C—CHEMISTRY; METALLURGY
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

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Description

【０００１】
【発明の属する技術分野】
本発明は、顕微鏡を用いて被観察物を長時間、例えば２４時間以上にわたって観察するために、恒温、恒湿度、恒気相を実現する装置に関するものである。上記被観察物は例えば生きた細胞である。
【０００２】
【従来の技術】
従来から、例えばプラスチック製容器に微生物を培養したペトリ皿を入れ、これを顕微鏡にセットし、微生物を経時的に撮影することによって、それら微生物の動きを観察することが行われている。このような観察は、本来、微生物が恒常的な環境で生存していることが前提である。
【０００３】
しかしながら顕微鏡観察下という特殊な状況下では、顕微鏡へ放熱があったり、顕微鏡の機械機構の動きがあったりなどの理由のために、微生物が恒常的な環境にあるとは言いがたい。
【０００４】
そのような顕微鏡観察に従来公知の静置型培養装置の技術を転用することも事実上不可能であった。例えばアルプ株式会社から市販されている恒温器は内部温度を３７℃程度で、内部温度差を１℃程度以下に制御するようになっており、この種の静置型培養装置は温度、湿度、ＣＯ２濃度を制御した培養環境を提供するが、実験器具や試料をケース内に収納した上、培養装置内部の所定位置にセットして実験を行う構成となっているために、顕微鏡操作のために付加的な構造が必要となり、金銭的負担が飛躍的に増加し、実機として得ることができない。
【０００５】
また有限会社トッケン等から市販されている顕微鏡載置型細胞培養容器は、温度、湿度などを調整した気体を循環させるものが殆どであり、これは振動源となるために、顕微鏡観察下、特にＣＣＤカメラなどにて撮像する条件では使用できない。そのような場合には目視による観察にしか容認されていなかった。
【０００６】
【発明が解決しようとする課題】
以上のことから本発明の根底をなす課題は、顕微鏡載置観察にあって被観察物が定常状態で存続できる恒温・恒湿度状態を提供できるようにすることにある。
【０００７】
【課題を解決するための手段】
この課題は本発明に従い、顕微鏡に載置した培養容器を収容し保温保湿する内部空間を有し且つ顕微鏡に取り付けることができるサイズに構成され、上記内部空間の温度を制御すべく周面部分と上面に調温手段を備え、上記内部空間の湿度を制御するための手段を内包し、更に顕微鏡の対物レンズを上記内部空間に位置させ、当該対物レンズの観察面側をエアカーテンで構成する恒温・恒湿装置により解決される。上記恒温状態は例えば３７℃±１℃であり、恒湿状態は相対湿度８０％以上であって、更には内部空間内のＣＯ２濃度が５％程度に調節される。
【０００９】
【発明の実施の形態】
以下に、本発明を好適な実施の形態を基に、図面を用いて詳細に説明する。
図１に、倒立型顕微鏡１に対して本発明に係る恒温・恒湿装置を装着した状態を概略的に示す。本発明に係る恒温・恒湿装置は、顕微鏡１の本体に載置される直方体形状のインキュベータ２を備えて成っている。このインキュベータ２は、顕微鏡１の対物レンズ３上の観察ステージに配されマイクロプレート４にある被観察対象をすっぽり覆い、対物レンズ３と共に被対象物を閉鎖空間に閉じこめるようになっている。
【００１０】
インキュベータ２は、観察ステージを境に、アッパー容器部分２ａとロウアー容器部分２ｂに分けられる。これら容器部分２ａ，２ｂは側面を自律温度調整機能を有した金属板で構成されている。例えば金属板側面に、ヒータ５を備えた断熱材６が取り付けられることで自律温度調整機能を備えるようにする。図示の例では、アッパー容器部分２ａの側面にのみヒータが備えられているが、ロウアー容器部分２ｂにもヒータが備えられていてもよいのは当然である。ロウアー容器部分２ｂの側面は顕微鏡レンズ部に対するカバー機能を果たしている。
【００１１】
アッパー容器部分２ａの上面は、自律温度調節発熱ガラス７で覆われている。またアッパー容器部分２ａの内部空間側に温度・湿度センサ８が取り付けられ、恒温恒湿の監視を行っている。
【００１２】
アッパー容器部分２ａのロウアー容器部分２ｂとの境界近傍で内側空間側には貯水部９が設けられている。必要に応じて外部から水を供給できるように、温度調整された無菌水を循環できるポンプ付き給水タンク１０が備えられている。この給水タンク１０はＳＵＳ製で、２０リットルの水を貯留できるようになっており、オーバーフロー方式でインキュベータ２の貯水部９に循環給水する。これによりアッパー容器部分２ａの内部空間における相対湿度をほぼ飽和状態にすることができる。結露廃液は排出部から廃棄されるようになっている。
【００１３】
ロウアー容器部分２ｂの内部空間、レンズ３の近傍に自律温度調節エアカーテンを構成する。これは、インキュベータ２の外部に設置されたＳＵＳ製のエアタンク１１からブロアー１２を用いて温度調整されたエアを、ロウアー容器部分２ｂのアッパー容器部分２ａとの境界近傍（観察面）へ噴出することで実現し、レンズ３近傍の気体条件（温度、湿度、気相濃度）を一定に保つことができる。
【００１４】
図２に、正立型顕微鏡のための本発明に係る恒温・恒湿装置を概略的に示す。本例の恒温・恒湿装置は、試料載置台２０を収容する円筒部２１とこれを担持する容器床板２２から成っている。試料載置台２０は、試料の大きさ或いはペトリ皿の大きさに応じて上下に高さを変えることができるようになっている。円筒部２１の側面は、上記の例におけるインキュベータと同じく、自律温度調整機能を有した金属板で構成されている。円筒部２１の上面は、自律温度調節発熱ガラス２３で構成されている。円筒部２１の内部で、容器床板２２に接し、試料載置台２０の周囲には水路２４が形成され、ここに水を溜めることができ、更に必要に応じて外部から給水できるようになっている。その結果、円筒部２１の内部空間は相対湿度がほぼ飽和状態に調整されている。円筒部２０の上部側方には、混合気体取り入れ口２５と排出口２６が取り付けられている。
【００１５】
【発明の効果】
本発明によれば、顕微鏡に載置される恒温・恒湿容器自体が温度調節機構を備えており、容器内部の温度ムラをなくし、また容器内部の温度ムラがなくなることで湿度も一定に保つことができることになって、被観察物が定常状態で長期間にわたり生存することができるようになる。
【図面の簡単な説明】
【図１】本発明に係る倒立顕微鏡用恒温・恒湿装置の概略図である。
【図２】本発明に係る正立顕微鏡用恒温・恒湿装置の概略図である。
【符号の説明】
１ 倒立顕微鏡本体
２ インキュベータ
２ａ アッパー容器部分
２ｂ ロウアー容器部分
３ 対物レンズ
４ マイクロプレート
５ ヒータ
６ 断熱材
７ 発熱ガラス
８ 温度・湿度センサ
９ 貯水部
１０ 給水タンク
１１ エアタンク
１２ ブロアー[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an apparatus for realizing constant temperature, constant humidity, and constant gas phase in order to observe an object to be observed for a long time, for example, 24 hours or more using a microscope. The observation object is, for example, a living cell.
[0002]
[Prior art]
2. Description of the Related Art Conventionally, for example, a petri dish in which microorganisms are cultured is placed in a plastic container, set in a microscope, and images of the microorganisms are observed over time to observe the movement of the microorganisms. Such observation is premised on the fact that microorganisms are alive in a constant environment.
[0003]
However, under special circumstances such as observation under a microscope, it is difficult to say that microorganisms are in a constant environment due to heat radiation to the microscope or movement of the mechanical mechanism of the microscope.
[0004]
It is virtually impossible to divert the technique of a conventionally known stationary culture apparatus to such microscopic observation. For example, a thermostat marketed by Alp Co., Ltd. has an internal temperature of about 37 ° C., and the internal temperature difference is controlled to about 1 ° C. or less. It provides a culture environment with a controlled 2 concentration, on housing the labware and sample into the case, because it is configured to perform experiments is set to a predetermined position inside the culture device, for microscopic manipulation An additional structure is required, the financial burden increases dramatically, and cannot be obtained as a real machine.
[0005]
In addition, most of the microscope-mounted cell culture vessels that are commercially available from Tokken Co., Ltd. circulate a gas whose temperature and humidity are adjusted. This is a source of vibration, so that it can be used under microscopic observation, especially CCD. It cannot be used under conditions where images are taken with a camera. In such a case, it was accepted only by visual observation.
[0006]
[Problems to be solved by the invention]
From the above, the problem that forms the basis of the present invention is to provide a constant temperature / humidity state in which the object to be observed can survive in a steady state in the microscope-mounted observation.
[0007]
[Means for Solving the Problems]
In accordance with the present invention, this object has an internal space for accommodating a culture vessel placed on a microscope and is kept warm and moisturized, and is configured to have a size that can be attached to a microscope, and a peripheral surface portion to control the temperature of the internal space comprising a temperature control means on the upper surface, and enclosing means for controlling the humidity of the internal space, further microscope objective is positioned in the interior space, constituting the observation surface side of the objective lens in air curtain thermostatic・ It is solved by a constant humidity device. The constant temperature state is, for example, 37 ° C. ± 1 ° C., the constant humidity state is a relative humidity of 80% or more, and the CO 2 concentration in the internal space is adjusted to about 5%.
[0009]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, the present invention will be described in detail with reference to the drawings based on preferred embodiments.
FIG. 1 schematically shows a state in which the constant temperature / humidity device according to the present invention is attached to an inverted microscope 1. The constant temperature / humidity device according to the present invention includes a rectangular parallelepiped incubator 2 placed on the main body of the microscope 1. The incubator 2 is arranged on the observation stage on the objective lens 3 of the microscope 1 so as to completely cover the object to be observed on the microplate 4, and to close the object together with the objective lens 3 in a closed space.
[0010]
The incubator 2 is divided into an upper container portion 2a and a lower container portion 2b with an observation stage as a boundary. These container parts 2a and 2b are formed of metal plates having an autonomous temperature adjustment function on the side surfaces. For example, the heat insulating material 6 provided with the heater 5 is attached to the side surface of the metal plate to provide an autonomous temperature adjustment function. In the illustrated example, the heater is provided only on the side surface of the upper container part 2a, but it is natural that the lower container part 2b may be provided with a heater. The side surface of the lower container portion 2b serves as a cover for the microscope lens portion.
[0011]
The upper surface of the upper container portion 2a is covered with an autonomous temperature control heating glass 7. Further, a temperature / humidity sensor 8 is attached to the inner space side of the upper container portion 2a to monitor constant temperature and humidity.
[0012]
A water reservoir 9 is provided in the inner space near the boundary between the upper container portion 2a and the lower container portion 2b. A water supply tank 10 with a pump capable of circulating the temperature-controlled sterile water is provided so that water can be supplied from the outside as required. This water supply tank 10 is made of SUS and can store 20 liters of water, and circulates and supplies water to the water storage unit 9 of the incubator 2 by an overflow method. Thereby, the relative humidity in the internal space of the upper container portion 2a can be almost saturated. Condensation waste liquid is discarded from the discharge section.
[0013]
An autonomous temperature control air curtain is formed in the inner space of the lower container portion 2 b and in the vicinity of the lens 3. This is because the temperature-adjusted air is blown from the air tank 11 made of SUS installed outside the incubator 2 to the vicinity (observation surface) of the lower container portion 2b with the upper container portion 2a. The gas conditions (temperature, humidity, gas phase concentration) in the vicinity of the lens 3 can be kept constant.
[0014]
FIG. 2 schematically shows a constant temperature and humidity device according to the present invention for an upright microscope. The constant temperature / humidity device of this example includes a cylindrical portion 21 that accommodates the sample mounting table 20 and a container floor plate 22 that carries the cylindrical portion 21. The height of the sample mounting table 20 can be changed vertically depending on the size of the sample or the size of the Petri dish. The side surface of the cylindrical portion 21 is formed of a metal plate having an autonomous temperature adjustment function, like the incubator in the above example. The upper surface of the cylindrical portion 21 is composed of an autonomous temperature control heat generating glass 23. A water channel 24 is formed around the sample mounting table 20 in contact with the container floor plate 22 inside the cylindrical portion 21 so that water can be stored therein, and water can be supplied from the outside as required. . As a result, the relative humidity of the internal space of the cylindrical portion 21 is adjusted to be almost saturated. A mixed gas inlet 25 and an outlet 26 are attached to the upper side of the cylindrical portion 20.
[0015]
【The invention's effect】
According to the present invention, the thermostatic / humidity container itself placed on the microscope is equipped with a temperature control mechanism to eliminate the temperature irregularity inside the container and to keep the humidity constant by eliminating the temperature irregularity inside the container. As a result, the object to be observed can survive in a steady state for a long period of time.
[Brief description of the drawings]
FIG. 1 is a schematic view of a constant temperature and humidity device for an inverted microscope according to the present invention.
FIG. 2 is a schematic view of a constant temperature and humidity device for an upright microscope according to the present invention.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 Inverted microscope main body 2 Incubator 2a Upper container part 2b Lower container part 3 Objective lens 4 Microplate 5 Heater 6 Heat insulating material 7 Heat generation glass 8 Temperature / humidity sensor 9 Water storage part 10 Water supply tank 11 Air tank 12 Blower

Claims (1)

顕微鏡に載置した培養容器を収容し保温保湿する内部空間を有し且つ顕微鏡に取り付けることができるサイズに構成され、上記内部空間の温度を制御すべく周面部分と上面に調温手段を備え、上記内部空間の湿度を制御するための手段を内包し、更に顕微鏡の対物レンズを上記内部空間に位置させ、当該対物レンズの観察面側をエアカーテンで構成することを特徴とする、培養容器内の被観察物を長時間生かすことができる恒温・恒湿装置。Containing a culture vessel placed on the microscope and having an internal space for keeping it warm and moisturized, and configured to be sized to be attached to the microscope, and equipped with temperature control means on the peripheral surface and the upper surface to control the temperature of the internal space A culture vessel comprising a means for controlling the humidity of the internal space , further comprising an objective lens of a microscope located in the internal space, and an observation surface side of the objective lens being constituted by an air curtain A constant temperature / humidity device that can make use of the object to be observed for a long time.
JP2002364234A 2002-12-16 2002-12-16 A constant temperature / humidity device that can keep the observation object in the culture vessel placed on the microscope alive for a long time. Expired - Lifetime JP4180902B2 (en)

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CN112210470B (en) * 2020-10-14 2021-06-29 太仓艺斯高医疗器械科技有限公司 Micro-vibration type constant humidity type thermostat
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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