JP3652049B2 - Wiping device for environmental microbiological examination - Google Patents

Wiping device for environmental microbiological examination Download PDF

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JP3652049B2
JP3652049B2 JP3819797A JP3819797A JP3652049B2 JP 3652049 B2 JP3652049 B2 JP 3652049B2 JP 3819797 A JP3819797 A JP 3819797A JP 3819797 A JP3819797 A JP 3819797A JP 3652049 B2 JP3652049 B2 JP 3652049B2
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紀也 木村
久夫 鶴見
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栄研器材株式会社
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube

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  • Sampling And Sample Adjustment (AREA)
  • Packaging Of Annular Or Rod-Shaped Articles, Wearing Apparel, Cassettes, Or The Like (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Description

【０００１】
【発明の属する技術分野】
本発明は、医療機関や食品製造所及び医薬品製造所などで使用される環境微生物検査用拭き取り器具に関するものである。
【０００２】
【従来の技術】
環境微生物検査には簡易な検査法が開発されており、培養を主体とする従来法と、微生物による汚染度を判定できる試薬を使った方法などがある。
【０００３】
培養を主体とする従来法においては、一般的に拭き取り法（スワブ法とも呼ばれている。）によって、物体表面の付着菌を綿棒などで拭き取る操作が実施されている。例えば、機器，器具，作業台などの物体の表面を、リン酸緩衝液あるいは生理食塩水等の稀釈液の一定量を湿らせた綿棒を用いて拭き取る操作が実施されている。これを同一の溶液を収容した合成樹脂製の容器に入れ、激しく振り動かすか、または殺菌力を持たない程度の弱い波長の超音波で振り動かす。この浸出液を必要に応じて稀釈し培養後、出現した細菌の集落を計測し、拭き取った面積の菌数を算出する。
【０００４】
拭き取り法は、広い面積以外に、手指や曲り部、隙間でも拭き取ることができる利点がある。
【０００５】
【発明が解決しようとする課題】
しかしながら、綿棒などを用いた検体採取のための拭き取り用具は、使用時にその綿球などの拭き取り用含浸体に過剰に含浸されているリン酸緩衝液あるいは生理食塩水などの稀釈液の絞り操作が必要である。即ち、検査をしようとする箇所の付着菌を採取するためには、過剰な稀釈液が拭き取り用含浸体にあると、付着菌の採取ができなくなり、正しい採取法にならない。
【０００６】
本発明の目的は、過剰な稀釈液を容易に除去できる環境微生物検査用拭き取り器具を提供することにある。
【０００７】
本発明の他の目的は、過剰な稀釈液を容易に除去できるスポイトを容易に低コストで形成できる環境微生物検査用拭き取り器具を提供することにある。
【０００８】
本発明の他の目的は、過剰な稀釈液を容易に除去できるスポイトを適切な素材を選択して形成できる環境微生物検査用拭き取り器具を提供することにある。
【０００９】
【課題を解決するための手段】
請求項１に記載の発明は、合成樹脂製の容器本体と、該容器本体を密封するスポイト付き蓋体とを備え、該スポイト付き蓋体の前記スポイトの前記容器本体内に挿入される管部の先端には拭き取り用含浸体が設けられていることを特徴とする。
【００１０】
このように蓋体がスポイト付き蓋体となっていて、該スポイト付き蓋体のスポイトの容器本体内に挿入される管部の先端には拭き取り用含浸体が設けられていると、使用時に容器本体内の稀釈液に浸漬されていて拭き取り用含浸体に過剰に稀釈液が含浸されていても、スポイトの空気溜め部を圧縮して内部の空気をスポイトの管部を経て吹き出させることにより、拭き取り用含浸体内の過剰な稀釈液を吐き出させることができ、簡単に適度な稀釈液を拭き取り用含浸体に保持させることができる。このため拭き取りによる物体表面からの付着菌の採取を正しく行うことができる。
【００１１】
請求項２に記載の発明は、前記スポイト付き蓋体が合成樹脂で一体成形されていることを特徴とする。
【００１２】
このようにすると、一体成形で容易に低コストでスポイト付き蓋体を形成することができる。容器本体とスポイト付き蓋体は、例えば高密度ポリエチレンなどの軟質の合成樹脂で形成することが好ましい。このような合成樹脂によれば、高圧蒸気滅菌や放射線滅菌などにも耐えさせることができる。
【００１３】
請求項３に記載の発明は、前記スポイト付き蓋体が蓋部とスポイトとを別部品にして構成されていることを特徴とする。
【００１４】
このようにすると、蓋部とスポイトとを、その用途から適切な素材を選択して形成することができる。
【００１５】
請求項４に記載の発明は、前記拭き取り用含浸体が発泡合成樹脂で構成されていることを特徴とする。
【００１６】
請求項５に記載の発明は、前記拭き取り用含浸体が天然の海綿で構成されていることを特徴とする。
【００１７】
請求項６に記載の発明は、前記拭き取り用含浸体が天然或いは化学繊維で構成されていることを特徴とする。
【００１８】
【発明の実施の形態】
図１乃至図３は、本発明に係る環境微生物検査用拭き取り器具における実施の形態の第１例を示したものである。
【００１９】
この環境微生物検査用拭き取り器具は、容器本体１と、該容器本体１を密封するスポイト付き蓋体２とを備え、該スポイト付き蓋体２のスポイト３の容器本体１内に挿入される管部３ａの先端には拭き取り用含浸体４が設けられている。容器本体１とスポイト付き蓋体２とは、いずれも例えば高密度ポリエチレンなどの軟質の合成樹脂で形成されている。これら容器本体１と、該容器本体１を密封するスポイト付き蓋体２とは、透明或いは不透明のいずれであってもよいが、後述するようにスポイト付き蓋体２をした状態で容器本体１内部での拭き取り用含浸体４中の過剰な稀釈液の押し出し操作と、付着菌の回収工程を確認するという観点から、少なくとも容器本体１は透明であることが好ましい。
【００２０】
容器本体１は有底の管状に形成され、その入口１ａの内面には雌ネジ部１ｂが形成されている。容器本体１内の底部側には、リン酸緩衝液あるいは生理食塩水等の稀釈液５が収容されている。
【００２１】
スポイト付き蓋体２は、蓋部６とスポイト３とで構成されている。蓋部６は、容器本体１の入口１ａに嵌合される嵌合部６ａと、嵌合部６ａに一体の蓋本体部６ｂとで構成され、嵌合部６ａの外周には容器本体１の入口１ａの雌ネジ部１ｂに螺合される雄ネジ部６ｃが形成され、嵌合部６ａの表面には複数本の滑り止め溝６ｄが形成されている。スポイト３は、中空の空気溜め部３ｂと、この空気溜め部３ｂに一体の前述した管部３ｂと、この管部３ｂの先端に一体に形成されている通気形含浸体取り付け部３ｃとで構成されている。この例のスポイト３の空気溜め部３ｂは、図３に示すように蓋部６の嵌合部６ａとこれに一体の蓋本体部６ｂを中空の可撓性容器として形成することにより、蓋部６と兼用された構造になっている。通気形含浸体取り付け部３ｃは中空の楕円形容器状に形成され、この通気形含浸体取り付け部３ｃには多数の孔７が形成されている。この孔７は、小さな多数の丸孔により形成されている。
【００２２】
拭き取り用含浸体４は、稀釈液５により劣化が生ぜず且つ耐摩耗性のあるを発泡ポリウレタンや発泡ポリエチレンなどのスポンジ状の発泡合成樹脂で構成されている。このような拭き取り用含浸体４は、スポイト３の通気形含浸体取り付け部３ｃに嵌め込んで水不溶性の合成接着剤で固定されている。
【００２３】
このような構造の環境微生物検査用拭き取り器具は、リン酸緩衝液あるいは生理食塩水等の稀釈液５が収容された容器本体１内にスポイト付き蓋体２の先端側を挿入することにより、拭き取り用含浸体４が稀釈液５中に浸漬され、蓋部６の嵌合部６ａが容器本体１の入口１ａに嵌合されて、容器本体１の入口１ａが蓋部６で閉塞される。蓋部６の嵌合部６ａを容器本体１の入口１ａに捩じ込むことにより嵌合部６ａの雄ネジ部６ｃが容器本体１の入口１ａ内面の雌ネジ部１ｂに螺合され、外れない状態になる。この状態で、高圧蒸気や放射線などで滅菌する。かかる状態で、保管や搬送が行われる。
【００２４】
検体採取現場では、容器本体１からスポイト付き蓋体２を外し、容器本体１内で拭き取り用含浸体４が稀釈液５の外に出た状態で、スポイト３の空気溜め部３ｂを圧縮して内部の空気をスポイト３の管部３ｂを経て通気形含浸体取り付け部３ｃの各孔７から吹き出させることにより、拭き取り用含浸体４内の過剰な稀釈液５を吐き出させることができ、簡単に適度な稀釈液５を拭き取り用含浸体４に保持させることができる。空気溜め部３ｂの圧縮は、１回で不十分なときには数回繰り返えせばよい。このようにして拭き取り用含浸体４に対する稀釈液５の含浸量を適量にすると、拭き取りによる物体表面からの付着菌の採取を正しく行うことができる。
【００２５】
また、本例のようにスポイト付き蓋体２が合成樹脂で一体成形されていると、一体成形で容易に低コストでスポイト付き蓋体２を形成することができる。容器本体１とスポイト付き蓋体２が前述したように高密度ポリエチレンなどの軟質の合成樹脂で形成されていると、高圧蒸気滅菌や放射線滅菌などにも耐えさせることができる。
【００２６】
また、拭き取り用含浸体４を発泡合成樹脂で構成すると、ほつれ或いは滅菌による熱や放射線に対し影響のない拭き取り用含浸体４とすることができる。拭き取り用含浸体４がほつれないと、板やざる等の表面が粗い物体の表面を拭いても、拭き取り後に現場に繊維が残ったり、培養地にほつれた繊維が存在して菌の集落数の妨げになったりするのを回避することができる。
【００２７】
図４、図５は、図３に示すスポイト付き蓋体２の変形例を示したものである。このスポイト付き蓋体２では、通気形含浸体取り付け部３ｃに設けられている孔７が複数本のスリットにより形成されている。その他の構成は、図３と同様な構成になっている。
【００２８】
図６乃至図８は、本発明に係る環境微生物検査用拭き取り器具における実施の形態の第２例を示したものである。
【００２９】
この環境微生物検査用拭き取り器具は、容器本体１と、該容器本体１を密封するスポイト付き蓋体２とを備え、該スポイト付き蓋体２のスポイト３の容器本体１内に挿入される管部３ａの先端には拭き取り用含浸体４が設けられている点は前述した第１例と同様である。
【００３０】
容器本体１は、入口１ａを有する上端が小径部１ｃとなって、その外周には雄ネジ部１ｄが突設されている。このような容器本体１内の底部側には、リン酸緩衝液あるいは生理食塩水等の稀釈液５が収容されている。
【００３１】
スポイト付き蓋体２は、蓋部６とスポイト３とが例えば高密度ポリエチレンなどの軟質の合成樹脂により一体成形で形成されている。スポイト３は、中空の空気溜め部３ｂと、この空気溜め部３ｂに一体の前述した管部３ａと、この管部３ａの先端に一体に形成されている通気形含浸体取り付け部３ｃとを備えている。通気形含浸体取り付け部３ｃは第１例と同様に中空の楕円形容器状に形成され、この通気形含浸体取り付け部３ｃには多数の孔７が小さな丸孔により形成されている。蓋部６は、スポイト３の空気溜め部３ｂの下で管部３ａの外周にキャップ状に一体成形されている。このキャップ状をした蓋部６の内周には、容器本体１の上端の小径部１ｃ外周に設けられている雄ネジ部１ｄに螺合させる雌ネジ部６ｅが設けられている。蓋部６の外周には、複数本の滑り止め溝６ｄが形成されている。
【００３２】
拭き取り用含浸体４は、第１例と同様に稀釈液５により劣化が生ぜず且つ耐摩耗性のあるを発泡ポリウレタンや発泡ポリエチレンなどのスポンジ状の発泡合成樹脂で構成されている。この拭き取り用含浸体４も、スポイト３の通気形含浸体取り付け部３ｃに嵌め込んで水不溶性の合成接着剤で固定されている。このような第２例の環境微生物検査用拭き取り器具も第１例と、同様な使用の仕方で、同様な効果を得ることができる。
【００３３】
図９、図１０は、図８に示すスポイト付き蓋体２の変形例を示したものである。
【００３４】
図９に示すスポイト付き蓋体２では、スポイト３の空気溜め部３ｂが管部３ａ側とは別体に形成されている。空気溜め部３ｂは例えば可撓性と復元性のよいゴムで形成され、管部３ａは前述したと同様に高密度ポリエチレンなどの軟質の合成樹脂で形成されている。空気溜め部３ｂは管部３ａの端部に嵌着され、管部３ａの端部に設けられた鍔３ｄで抜け止めされている。このようにスポイト３の空気溜め部３ｂと管部３ａ側とを別体に形成すると、各側を最適な材質のもので形成することができる。
【００３５】
図１０に示すスポイト付き蓋体２では、蓋部６とスポイト３とが別体に形成され、両者はスポイト３の管部３ａの外周に蓋部６がネジ結合部８で取り付けられている。この場合も、蓋部６とスポイト３とは、各側を最適な材質のもので形成することができる。また、蓋部６の色を適宜変えることができる。
【００３６】
図１１は、スポイト３の変形例を示したものである。このスポイト３では、通気形含浸体取り付け部３ｃが管部３ａと同様に同じ外径で管状に形成され、この通気形含浸体取り付け部３ｃではその先端が管部３ａと同じ内径で開口され、この通気形含浸体取り付け部３ｃには多数の孔７が小さな丸孔により形成されている。
【００３７】
上記各例では、拭き取り用含浸体４を発泡ポリウレタンや発泡ポリエチレンなどのスポンジ状の発泡合成樹脂で構成した場合について示したが、この拭き取り用含浸体４は天然の海綿や、天然或いは化学繊維で構成することもできる。このような天然の拭き取り用含浸体４も、スポイト３の通気形含浸体取り付け部３ｃに水不溶性の合成接着剤で固定することができる。
【００３８】
【発明の効果】
本発明に係る環境微生物検査用拭き取り器具では、容器本体を密封する蓋体がスポイト付き蓋体となっていて、該スポイト付き蓋体のスポイトの容器本体内に挿入される管部の先端には拭き取り用含浸体が設けられているので、使用時に容器本体内の稀釈液に浸漬されていて拭き取り用含浸体に過剰に稀釈液が含浸されていても、スポイトの空気溜め部を圧縮して内部の空気をスポイトの管部を経て吹き出させることにより、拭き取り用含浸体内の過剰な稀釈液を吐き出させることができ、簡単に適度な稀釈液を拭き取り用含浸体に保持させることができる。このため拭き取りによる物体表面からの付着菌の採取を正しく行うことができる。
【００３９】
また、拭き取り用含浸体に付着した微生物の回収工程においても、蓋体のスポイトを容器本体内に挿入し、スポイトの空気溜め部を押圧することによって拭き取り用含浸体全体に圧縮空気が発生し、拭き取り用含浸体に付着した微生物を効率良く回収でき、環境微生物による汚染度合いを厳格に把握することができる。
【図面の簡単な説明】
【図１】本発明に係る環境微生物検査用拭き取り器具における実施の形態の第１例を示した斜視図である。
【図２】第１例の環境微生物検査用拭き取り器具における容器本体とスポイト付き蓋体の分解状態の斜視図である。
【図３】図２に示すスポイト付き蓋体の縦断面図である。
【図４】図２に示すスポイト付き蓋体の変形例を示す斜視図である。
【図５】図４の縦断面図である。
【図６】本発明に係る環境微生物検査用拭き取り器具における実施の形態の第２例を示した斜視図である。
【図７】図６に示す環境微生物検査用拭き取り器具の縦断面図である。
【図８】図６に示す環境微生物検査用拭き取り器具のポイト付き蓋体の縦断面図である。
【図９】図７に示すポイト付き蓋体の変形例を示す縦断面図である。
【図１０】図７に示すポイト付き蓋体の変形例を示す縦断面図である。
【図１１】スポイトの変形例を示す要部縦断面図である。
【符号の説明】
１ 容器本体
１ａ 入口
１ｂ 雌ネジ部
１ｃ 小径部
１ｄ 雄ネジ部
２ スポイト付き蓋体
３ スポイト
３ａ 管部
３ｂ 空気溜め部
３ｃ 通気形含浸体取り付け部
３ｄ 鍔
４ 拭き取り用含浸体
５ 稀釈液
６ 蓋部
６ａ 嵌合部
６ｂ 蓋本体部
６ｃ 雄ネジ部
６ｄ 滑り止め溝
６ｅ 雌ネジ部
７ 孔
８ ネジ結合部[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a wiping device for testing environmental microorganisms used in medical institutions, food factories, pharmaceutical factories, and the like.
[0002]
[Prior art]
A simple test method has been developed for the environmental microbiological test, and there are a conventional method mainly using culture and a method using a reagent capable of determining the degree of contamination by microorganisms.
[0003]
In the conventional method mainly including culture, an operation of wiping off adherent bacteria on the surface of an object with a cotton swab or the like is generally performed by a wiping method (also called a swab method). For example, an operation of wiping the surface of an object such as a device, instrument, or workbench with a cotton swab moistened with a certain amount of a diluent such as phosphate buffer or physiological saline is performed. This is put in a synthetic resin container containing the same solution and shaken vigorously, or shaken with an ultrasonic wave having a weak wavelength not having sterilizing power. This exudate is diluted as necessary, and after cultivation, the colonies of the bacteria that appear are counted, and the number of bacteria in the wiped area is calculated.
[0004]
The wiping method has an advantage that it can be wiped even with fingers, bent portions, and gaps in addition to a wide area.
[0005]
[Problems to be solved by the invention]
However, a wiping tool for sample collection using a cotton swab, etc., can be used to squeeze a diluted solution such as a phosphate buffer or physiological saline that is excessively impregnated in the wiping impregnation body such as a cotton ball during use. is necessary. That is, in order to collect adhering bacteria at the location to be inspected, if there is an excessive diluted solution in the wiping impregnated body, the adhering bacteria cannot be collected and the correct collection method cannot be obtained.
[0006]
An object of the present invention is to provide a wiping device for testing environmental microorganisms that can easily remove excess diluted solution.
[0007]
Another object of the present invention is to provide a wiping device for testing environmental microorganisms that can easily form a dropper capable of easily removing excessive dilution liquid at low cost.
[0008]
Another object of the present invention is to provide a wiping device for testing environmental microorganisms that can form a dropper that can easily remove excess diluent by selecting an appropriate material.
[0009]
[Means for Solving the Problems]
The invention described in claim 1 includes a container body made of a synthetic resin, and a lid with a dropper that seals the container body, and a tube portion that is inserted into the container body of the dropper of the lid with a dropper A wiping impregnated body is provided at the tip of the substrate.
[0010]
In this way, the lid body is a lid body with a dropper, and when the impregnating body for wiping is provided at the tip of the tube portion inserted into the dropper container body of the lid with the dropper, Even if it is immersed in the diluent in the body and the impregnation body for wiping is excessively impregnated with the diluent, by compressing the air reservoir of the dropper and blowing the internal air through the dropper tube, Excess dilution liquid in the wiping impregnation body can be discharged, and an appropriate dilution liquid can be easily held in the wiping impregnation body. Therefore, it is possible to correctly collect the attached bacteria from the object surface by wiping.
[0011]
The invention according to claim 2 is characterized in that the lid body with a dropper is integrally formed of a synthetic resin.
[0012]
If it does in this way, a lid with a dropper can be easily formed at low cost by integral molding. The container body and the lid with a dropper are preferably formed of a soft synthetic resin such as high-density polyethylene. Such a synthetic resin can withstand high pressure steam sterilization and radiation sterilization.
[0013]
The invention according to claim 3 is characterized in that the lid body with a dropper is configured with a lid part and a dropper as separate parts.
[0014]
If it does in this way, an appropriate material can be selected and formed from a use for a lid part and a dropper.
[0015]
The invention according to claim 4 is characterized in that the wiping impregnated body is made of a foamed synthetic resin.
[0016]
The invention according to claim 5 is characterized in that the wiping impregnated body is made of natural sponge.
[0017]
The invention according to claim 6 is characterized in that the wiping impregnated body is composed of natural or chemical fibers.
[0018]
DETAILED DESCRIPTION OF THE INVENTION
FIG. 1 thru | or FIG. 3 shows the 1st example of embodiment in the wiping instrument for an environmental microbe test | inspection which concerns on this invention.
[0019]
This wiping device for testing environmental microorganisms includes a container body 1 and a lid body 2 with a dropper that seals the container body 1, and a tube portion that is inserted into the container body 1 of the dropper 3 of the lid body 2 with a dropper. A wiping impregnation body 4 is provided at the tip of 3a. Each of the container body 1 and the lid body 2 with a dropper is formed of a soft synthetic resin such as high-density polyethylene. The container body 1 and the lid body 2 with a dropper that seals the container body 1 may be either transparent or opaque. However, as will be described later, the interior of the container body 1 with the lid body 2 with a dropper is used. It is preferable that at least the container main body 1 is transparent from the viewpoint of confirming the operation of pushing out the excess diluted solution in the wiping impregnated body 4 and the step of collecting the attached bacteria.
[0020]
The container body 1 is formed in a bottomed tubular shape, and a female screw portion 1b is formed on the inner surface of the inlet 1a. A diluent 5 such as a phosphate buffer or physiological saline is accommodated on the bottom side in the container body 1.
[0021]
The lid body 2 with a dropper includes a lid portion 6 and a dropper 3. The lid portion 6 includes a fitting portion 6a that is fitted to the inlet 1a of the container body 1 and a lid body portion 6b that is integral with the fitting portion 6a. A male screw portion 6c that is screwed into the female screw portion 1b of the inlet 1a is formed, and a plurality of anti-slip grooves 6d are formed on the surface of the fitting portion 6a. The dropper 3 is composed of a hollow air reservoir portion 3b, the above-described tube portion 3b integrated with the air reservoir portion 3b, and a vent-type impregnated body attachment portion 3c integrally formed at the tip of the tube portion 3b. Has been. As shown in FIG. 3, the air reservoir 3b of the dropper 3 of this example is formed by forming a fitting portion 6a of the lid portion 6 and a lid body portion 6b integral therewith as a hollow flexible container. 6 is also used as a structure. The vent-type impregnated body attaching portion 3c is formed in a hollow elliptical container shape, and a plurality of holes 7 are formed in the vent-type impregnated body attaching portion 3c. The hole 7 is formed by a large number of small round holes.
[0022]
The wiping impregnated body 4 is made of a sponge-like foamed synthetic resin such as foamed polyurethane or foamed polyethylene, which is not deteriorated by the diluent 5 and has wear resistance. Such a wiping impregnated body 4 is fitted into a breathable impregnated body attaching portion 3c of a dropper 3 and fixed with a water-insoluble synthetic adhesive.
[0023]
The wiping device for testing environmental microorganisms having such a structure is wiped by inserting the tip end side of the lid body 2 with a dropper into the container body 1 in which a diluent 5 such as phosphate buffer or physiological saline is accommodated. The impregnated body 4 is immersed in the diluent 5, the fitting portion 6 a of the lid portion 6 is fitted to the inlet 1 a of the container body 1, and the inlet 1 a of the container body 1 is closed by the lid portion 6. By screwing the fitting portion 6a of the lid portion 6 into the inlet 1a of the container main body 1, the male screw portion 6c of the fitting portion 6a is screwed to the female screw portion 1b on the inner surface of the inlet 1a of the container main body 1 and does not come off. It becomes a state. In this state, sterilize with high-pressure steam or radiation. In such a state, storage and conveyance are performed.
[0024]
At the specimen collection site, the lid body 2 with the dropper is removed from the container body 1, and the air reservoir 3 b of the dropper 3 is compressed in a state where the impregnating body 4 for wiping out of the dilution liquid 5 in the container body 1. By blowing out the internal air from each hole 7 of the vent-type impregnated body attachment portion 3c through the pipe portion 3b of the dropper 3, the excessive diluted solution 5 in the wiping impregnated body 4 can be discharged, easily. An appropriate diluent 5 can be held on the impregnating body 4 for wiping. The compression of the air reservoir 3b may be repeated several times when it is insufficient once. When the amount of the diluted solution 5 impregnated in the wiping impregnated body 4 is set to an appropriate amount in this way, it is possible to correctly collect the attached bacteria from the object surface by wiping.
[0025]
Moreover, when the lid body 2 with a dropper is integrally formed of synthetic resin as in this example, the lid body 2 with a dropper can be easily formed at a low cost by integral molding. When the container body 1 and the lid body 2 with a dropper are formed of a soft synthetic resin such as high-density polyethylene as described above, they can withstand high-pressure steam sterilization or radiation sterilization.
[0026]
Moreover, when the wiping impregnation body 4 is made of a foamed synthetic resin, the wiping impregnation body 4 having no influence on fraying or sterilization heat or radiation can be obtained. If the impregnated body 4 for wiping is not frayed, even if the surface of a rough object such as a plate or a pestle is wiped, fibers will remain on the site after wiping, or fibers that have frayed in the culture medium will be present and It is possible to avoid obstructing.
[0027]
4 and 5 show modifications of the lid body 2 with a dropper shown in FIG. In the lid body 2 with a dropper, the hole 7 provided in the vent-type impregnated body attaching portion 3c is formed by a plurality of slits. Other configurations are the same as those in FIG.
[0028]
FIGS. 6 to 8 show a second example of the embodiment of the wiping device for testing environmental microorganisms according to the present invention.
[0029]
This wiping device for testing environmental microorganisms includes a container body 1 and a lid body 2 with a dropper that seals the container body 1, and a tube portion that is inserted into the container body 1 of the dropper 3 of the lid body 2 with a dropper. The point that the impregnating body 4 for wiping is provided at the tip of 3a is the same as in the first example described above.
[0030]
The container body 1 has an upper end having an inlet 1a as a small diameter portion 1c, and a male screw portion 1d projecting from the outer periphery thereof. A dilution liquid 5 such as a phosphate buffer or physiological saline is accommodated on the bottom side in the container main body 1.
[0031]
In the lid body 2 with a dropper, the lid portion 6 and the dropper 3 are integrally formed of a soft synthetic resin such as high-density polyethylene. The dropper 3 includes a hollow air reservoir portion 3b, the above-described tube portion 3a integrated with the air reservoir portion 3b, and a vent-type impregnated body attachment portion 3c formed integrally with the tip of the tube portion 3a. ing. The vent type impregnated body attaching portion 3c is formed in a hollow elliptical container shape as in the first example, and a large number of holes 7 are formed in the vent type impregnated body attaching portion 3c by small round holes. The lid portion 6 is integrally formed in a cap shape on the outer periphery of the tube portion 3a under the air reservoir portion 3b of the dropper 3. On the inner periphery of the cap-shaped lid portion 6, there is provided a female screw portion 6 e that is screwed into a male screw portion 1 d provided on the outer periphery of the small diameter portion 1 c at the upper end of the container body 1. A plurality of anti-slip grooves 6 d are formed on the outer periphery of the lid portion 6.
[0032]
As in the first example, the wiping impregnated body 4 is made of a sponge-like foamed synthetic resin such as polyurethane foam or polyethylene foam which does not deteriorate due to the diluent 5 and has wear resistance. The wiping impregnated body 4 is also fitted into the breathable impregnated body attaching portion 3c of the dropper 3 and fixed with a water-insoluble synthetic adhesive. Such a wiping device for testing environmental microorganisms in the second example can also obtain the same effect in the same manner of use as in the first example.
[0033]
9 and 10 show a modified example of the lid body 2 with a dropper shown in FIG.
[0034]
In the lid body 2 with a dropper shown in FIG. 9, the air reservoir 3b of the dropper 3 is formed separately from the pipe 3a side. The air reservoir 3b is formed of, for example, rubber having good flexibility and resilience, and the tube 3a is formed of a soft synthetic resin such as high-density polyethylene as described above. The air reservoir 3b is fitted to the end of the tube 3a, and is prevented from coming off by a flange 3d provided at the end of the tube 3a. Thus, if the air reservoir 3b and the pipe portion 3a side of the dropper 3 are formed separately, each side can be formed of an optimal material.
[0035]
In the lid body 2 with the dropper shown in FIG. 10, the lid portion 6 and the dropper 3 are formed separately, and the lid portion 6 is attached to the outer periphery of the tube portion 3 a of the dropper 3 with a screw coupling portion 8. Also in this case, the lid portion 6 and the dropper 3 can be formed of an optimum material on each side. Moreover, the color of the cover part 6 can be changed suitably.
[0036]
FIG. 11 shows a modified example of the dropper 3. In this dropper 3, the vent-type impregnated body attaching portion 3c is formed in a tubular shape with the same outer diameter as the tube portion 3a, and the tip of the vent-type impregnated body attaching portion 3c is opened with the same inner diameter as the pipe portion 3a, A large number of holes 7 are formed in the vent-type impregnated body attaching portion 3c by small round holes.
[0037]
In each of the above examples, the case where the wiping impregnated body 4 is composed of a sponge-like foamed synthetic resin such as foamed polyurethane or foamed polyethylene is shown, but the wiping impregnated body 4 is made of natural sponge, natural or chemical fiber. It can also be configured. Such a natural wiping impregnated body 4 can also be fixed to the vent-type impregnated body attaching portion 3c of the dropper 3 with a water-insoluble synthetic adhesive.
[0038]
【The invention's effect】
In the wiping device for environmental microbiological examination according to the present invention, the lid for sealing the container body is a lid with a dropper, and at the tip of the tube part inserted into the container body of the dropper of the lid with the dropper The wiper impregnated body is provided, so even if it is immersed in the diluent in the container body during use and the wiper impregnated body is excessively impregnated with the diluted liquid, the air reservoir of the dropper is compressed to the inside. The excess dilution liquid in the wiping impregnation body can be discharged by blowing out the air through the dropper tube, and an appropriate dilution liquid can be easily held in the wiping impregnation body. Therefore, it is possible to correctly collect the attached bacteria from the object surface by wiping.
[0039]
Also, in the recovery process of microorganisms attached to the wiping impregnated body, compressed air is generated in the entire wiping impregnated body by inserting the dropper of the lid into the container body and pressing the air reservoir of the dropper, Microorganisms adhering to the wiping impregnated body can be efficiently recovered, and the degree of contamination by environmental microorganisms can be strictly grasped.
[Brief description of the drawings]
FIG. 1 is a perspective view showing a first example of an embodiment of a wiping device for testing environmental microorganisms according to the present invention.
FIG. 2 is a perspective view of a disassembled state of a container body and a lid with a dropper in the wiping device for testing environmental microorganisms of the first example.
3 is a longitudinal sectional view of the lid body with a dropper shown in FIG. 2. FIG.
4 is a perspective view showing a modified example of the lid body with a dropper shown in FIG. 2. FIG.
5 is a longitudinal sectional view of FIG. 4. FIG.
FIG. 6 is a perspective view showing a second example of the embodiment of the wiping device for testing environmental microorganisms according to the present invention.
7 is a vertical cross-sectional view of the wiping device for testing environmental microorganisms shown in FIG. 6. FIG.
FIG. 8 is a longitudinal sectional view of a lid with a dropper of the wiping device for testing environmental microorganisms shown in FIG. 6;
FIG. 9 is a longitudinal sectional view showing a modified example of the lid with a dropper shown in FIG.
10 is a longitudinal sectional view showing a modified example of the lid with a dropper shown in FIG.
FIG. 11 is a longitudinal sectional view of an essential part showing a modified example of a dropper.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 Container main body 1a Inlet 1b Female thread part 1c Small diameter part 1d Male thread part 2 Lid with a dropper 3 Dropper 3a Pipe part 3b Air reservoir part 3c Aeration type impregnated body attaching part 3d 鍔 4 Wiping impregnated body 5 Diluted solution 6 Lid part 6a Fitting portion 6b Lid main body portion 6c Male screw portion 6d Non-slip groove 6e Female screw portion 7 Hole 8 Screw coupling portion

Claims (6)

容器本体と、該容器本体を密封するスポイト付き蓋体とを備え、該スポイト付き蓋体の前記スポイトの前記容器本体内に挿入される管部の先端には拭き取り用含浸体が設けられていることを特徴とする環境微生物検査用拭き取り器具。A container body and a lid with a dropper that seals the container body, and an impregnating body for wiping is provided at the tip of the tube portion of the lid with the dropper that is inserted into the container body. A wiping device for testing environmental microorganisms. 前記スポイト付き蓋体が合成樹脂で一体成形されていることを特徴とする請求項１に記載の環境微生物検査用拭き取り器具。The wiping instrument for testing environmental microorganisms according to claim 1, wherein the lid with a dropper is integrally formed of a synthetic resin. 前記スポイト付き蓋体が蓋部とスポイトとを別部品にして構成されていることを特徴とする請求項１に記載の環境微生物検査用拭き取り器具。The wiping device for testing environmental microorganisms according to claim 1, wherein the lid with a dropper is configured with a lid part and a dropper as separate parts. 前記拭き取り用含浸体が発泡合成樹脂で構成されていることを特徴とする請求項１，２または３に記載の環境微生物検査用拭き取り器具。The wiping instrument for environmental microbiological examination according to claim 1, wherein the wiping impregnated body is made of a foamed synthetic resin. 前記拭き取り用含浸体が天然の海綿で構成されていることを特徴とする請求項１，２または３に記載の環境微生物検査用拭き取り器具。The wiping instrument for environmental microbiological examination according to claim 1, 2 or 3, wherein the wiping impregnated body is made of natural sponge. 前記拭き取り用含浸体が天然或いは化学繊維で構成されていることを特徴とする請求項１，２または３に記載の環境微生物検査用拭き取り器具。4. The wiping instrument for environmental microbiological examination according to claim 1, wherein the wiping impregnated body is made of natural or chemical fiber.
JP3819797A 1997-02-21 1997-02-21 Wiping device for environmental microbiological examination Expired - Fee Related JP3652049B2 (en)

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NO311464B1 (en) * 1998-12-01 2001-11-26 Pedersen Bjergaard Stig Process and disposable micro-extraction device
WO2001028680A2 (en) * 1999-10-20 2001-04-26 Gentra Systems, Inc. Mixing and pouring apparatus with rotatable arm and related vessel
JP4642277B2 (en) * 2001-06-21 2011-03-02 株式会社ジーシー Saliva pretreatment tool and saliva pretreatment method
JP4640104B2 (en) * 2005-10-20 2011-03-02 パナソニック株式会社 Microorganism measuring apparatus and microorganism measuring method
JP4882451B2 (en) * 2006-03-30 2012-02-22 凸版印刷株式会社 Oral mucosa sampler
JP5290131B2 (en) * 2009-11-16 2013-09-18 デンカ生研株式会社 Swab for collecting biological specimen, method for producing the swab, and kit using the swab
DE102010023229A1 (en) * 2010-06-09 2011-12-15 Otto Berners Device and method for transporting and processing cellular material
JP5745378B2 (en) * 2011-10-04 2015-07-08 滋賀県 Biological sample collection kit
JP5764045B2 (en) * 2011-11-18 2015-08-12 株式会社エルメックス Wiping inspection kit
JP2013228235A (en) * 2012-04-24 2013-11-07 Shin Corporation:Kk Inspection kit
JP6665939B2 (en) * 2016-09-06 2020-03-13 株式会社島津製作所 Swab wood

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