JP3436768B2 - Culture and cultivation method of new strain - Google Patents

Description

【発明の詳細な説明】Detailed Description of the Invention

【０００１】[0001]

【産業上の利用分野】本発明は、担子菌の新菌株に関
し、更に詳しくはリオフィラム ウルマリウム（ Lyoph
yllum ulmarium )の新菌株の培養方法及び子実体の栽培
方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a new strain of basidiomycete, more specifically, Lyophyllum ulmarium
and a method for cultivating fruiting bodies of a new strain of yllum ulmarium).

【０００２】[0002]

【従来の技術】リオフィラム ウルマリウムは、自然界
においては秋期に種々の広葉樹の枯れ木に叢生あるいは
離生しており、従来より形や歯切れの良い肉質のため、
極めて美味なきのことして採食されてきた。また、近年
ではオガクズに米糠やその他の栄養源を配合した培養基
を用いて、ビン又は箱で栽培を行う菌床人工栽培法が確
立され、季節に関係なく一年を通じて安定してきのこを
収穫できるようになってきた。ここでリオフィラム ウ
ルマリウムの菌床人工栽培を簡略に説明すれば、ビン又
は箱に培養基を詰め蒸気滅菌したものに、予め同様の培
養基に菌糸を繁殖させておいた種菌を接種し、所定の日
数培養した後発生操作を行い、子実体を得て収穫して１
サイクルが終了する。栽培に使用する菌株として数種の
菌株が市販あるいは技術提携により供与されているが、
使用する菌株により様々な形態や栽培特性がある（特開
昭６３−２７３４６７号）。2. Description of the Related Art Riofilum ulmarium naturally grows into dead trees of various broad-leaved trees in the autumn, or vegetatively grows. Because of its shape and crispy flesh quality,
It has been eaten as an extremely delicious mushroom. In addition, in recent years, using a culture medium containing rice bran and other nutrients in sawdust, a fungal bed artificial cultivation method has been established in which cultivation is performed in bottles or boxes, and mushrooms can be stably harvested throughout the year regardless of the season. It's starting to happen. Briefly explaining artificial bed cultivation of riophyllum ulmarium here, a bottle or box filled with culture medium and steam sterilized is inoculated with inoculum in which hyphae have been propagated in the same culture medium beforehand, and cultured for a predetermined number of days. After that, carry out the generation operation to obtain fruit bodies and harvest 1
The cycle ends. Several strains are commercially available or provided by technical cooperation as strains used for cultivation,
There are various forms and cultivation characteristics depending on the strain used (Japanese Patent Laid-Open No. 63-273467).

【０００３】[0003]

【発明が解決しようとする課題】しかしながら、現在使
用されているリオフィラム ウルマリウム菌株のすべて
は収穫時の子実体中に苦味成分を含有しており、子実体
収量を増加させる高苦味化培地では食用に適さないほど
の苦味をもつ。また、この苦味成分は培地の種類により
変動を示し、苦味の強さに大きく差が生じる。一般にき
のこは素材の味を重視する蔬菜的使用方法が主流なた
め、苦味成分が多く含有されることは著しく商品価値を
低下させる結果となる。このために、たとえ子実体の収
穫量を著しく増加させる培地基材であっても、同時に苦
味成分を増加させる培地、すなわち高苦味化培地は使用
できないのが現状である。本発明の目的は、上記現状に
かんがみ、これら高苦味化培地で栽培しても収穫時の子
実体中の苦味成分が食用に供した場合苦味をほとんど感
じない程度以下の新菌株の培養及び栽培方法を提供する
ことにある。However, all of the currently used Riophyllum ulmarium strains contain bitterness components in the fruiting bodies at the time of harvesting, and are edible in a high bittering medium that increases the yield of fruiting bodies. Has an unsuitable bitterness. In addition, the bitterness component varies depending on the type of medium, and there is a large difference in the bitterness intensity. Generally, mushrooms are mainly used in a vegetable-like manner, which places importance on the taste of the raw material, so that a large amount of bitterness components results in a marked decrease in commercial value. For this reason, the present situation is that even a medium base material that significantly increases the yield of fruiting bodies, a medium that simultaneously increases the bitterness component, that is, a high bitterness medium cannot be used. In view of the present situation, the object of the present invention is to cultivate and culture these new bitterness strains with little bitterness when the bitterness component in the fruiting body at harvest is edible even when cultivated in these high bittering medium. To provide a method.

【０００４】[0004]

【課題を解決するための手段】本発明を概説すれば、本
発明の第１の発明はリオフィラム ウルマリウム新菌株
の培養方法に関する発明であって、高苦味化培地で栽培
して得られる子実体の苦味が、少なくとも一種の高苦味
化培地について、０．０００００８Ｍ 硫酸キニーネよ
りも苦くなく、かつ下記の菌株、又はこれらの変異株か
ら選択されるリオフィラム ウルマリウム新菌株を培地
に接種し、菌糸体を生成させることを特徴とする。 リオフィラム ウルマリウム Ｋ−０４２９（ＦＥＲＭ
Ｐ−１２５７０）、 リオフィラム ウルマリウム Ｋ−０２０７（ＦＥＲＭ
Ｐ−１２５６９）、 リオフィラム ウルマリウム Ｋ−０２０２（ＦＥＲＭ
Ｐ−１２９８０）、 リオフィラム ウルマリウム Ｋ−０２５９（ＦＥＲＭ
Ｐ−１２９８１）、 リオフィラム ウルマリウム Ｋ−１００４（ＦＥＲＭ
Ｐ−１２９８２）、 リオフィラム ウルマリウム Ｋ−１２５７（ＦＥＲＭ
Ｐ−１２９８３）、 リオフィラム ウルマリウム Ｋ−６８０４（ＦＥＲＭ
Ｐ−１２９８４）、 リオフィラム ウルマリウム Ｋ−６８０６（ＦＥＲＭ
Ｐ−１２９８５） また本発明の第２の発明はリオフィラム ウルマリウム
新菌株の子実体の栽培方法に関する発明であって、上記
第１の発明におけるリオフィラム ウルマリウム新菌株
を培地に接種し、子実体を生成させることを特徴とす
る。SUMMARY OF THE INVENTION The present invention will be summarized. The first invention of the present invention is a new strain of riophyllum ulmarium.
The invention relating to the culturing method, wherein the bitterness of the fruiting body obtained by culturing in the high bittering medium is less bitter than 0.000008M quinine sulfate for at least one high bittering medium, and the strains described below, or medium Lyophyllum ulmarium new strains are selected from these mutant strains
Inoculated into, characterized by Rukoto to produce a mycelium. Riofilum Ulmarium K-0429 (FERM
P-12570), Riofilum ulmarium K-0207 (FERM
P-12569), Riofilum ulmarium K-0202 (FERM
P-12980), Riofilum ulmarium K-0259 (FERM
P-12981), Riofilum ulmarium K-1004 (FERM
P-12982), Riofilum ulmarium K-1257 (FERM
P-12983), Riofilum ulmarium K-6804 (FERM
P-12984), Riofilum ulmarium K-6806 (FERM
A second invention of the P-12985) The present invention is an invention relating to a method of cultivating a fruit body of Lyophyllum ulmarium novel strain was inoculated to the medium Lyophyllum ulmarium novel strain in the first invention, the fruiting bodies generate It is characterized by

【０００５】本発明者らはリオフィラム ウルマリウム
子実体の官能検査を行い、子実体の苦味と栽培培地との
関係を明らかにし、子実体の苦味を増加させる培地、す
なわち高苦味化培地を用い人工栽培を行っても、得られ
る子実体の苦味が、少なくとも一種の高苦味化培地につ
いて、苦味をほとんど感じない程度以下であるリオフィ
ラム ウルマリウム新菌株を育種することに成功し、本
発明を完成させた。The present inventors conducted a sensory test on the fruiting body of Riofilum ulmarium to clarify the relationship between the bitterness of the fruiting body and the cultivation medium, and artificially cultivate it using a medium for increasing the bitterness of the fruiting body, that is, a high bittering medium. The present invention succeeded in breeding a new strain of Riofilum ulmarium, in which the bitterness of the fruiting body thus obtained was at least as high as the bitterness in at least one type of high-bittering medium, and the present invention was completed.

【０００６】以下、本発明を詳細に説明する。リオフィ
ラム ウルマリウム菌株として、特開昭６３−２７３４
６７号公報に記載のリオフィラム ウルマリウム Ｌｕ
１−２（ＦＥＲＭ Ｐ−１２５８４）、リオフィラム
ウルマリウム Ｌｕ １−８（ＦＥＲＭ ＢＰ−１４
１６）、リオフィラム ウルマリウム Ｌｕ １−１７
（ＦＥＲＭ ＢＰ−１４１７）、リオフィラム ウルマ
リウム Ｍ−８１７１（ＦＥＲＭ ＢＰ−１４１５）を
使用し、該公報に記載の方法により、各菌株の人工栽培
を行い、収穫適期の子実体を収穫し、子実体の官能検査
を行った。人工栽培の培地としては、該公報に記載の針
葉樹オガクズ５０ｇ、広葉樹オガクズ５０ｇ、米糠９０
ｇをよく混合し、水道水にて水分含有率６５％に調製し
たものを、ポリプロピレン製８５０ml容広口ビンに圧詰
めして、ビン口部中央より下方に向い直径１cmの穴をあ
けた後、キャップで打栓したオガクズ固型培養基を１２
０℃、６０分間高圧蒸気滅菌したものをＡ培地とした。
また該培地調製方法に準じ、コーンコブミール１３０
ｇ、マメカワ６０ｇ、フスマ３０ｇをよく混合して同様
に作成したものをＢ培地、針葉樹オガクズ５０ｇ、綿実
殻粉砕物５０ｇ、米糠１００ｇをよく混合して同様に作
成したものをＣ培地、針葉樹オガクズ１００ｇ、マメカ
ワ７０ｇをよく混合して同様に作成したものをＤ培地と
し、それぞれ用いた。各使用培地で得られた子実体の収
量（ｇ／ビン）、官能検査の結果を表１に示す。The present invention will be described in detail below. The lyophilum ulmarium strain is disclosed in JP-A-63-2734.
Riofilum ulmarium Lu described in Japanese Patent Publication No. 67
1-2 (FERM P-12584), Riofilum ulmarium Lu 1-8 (FERM BP-14
16), Riofilum Ulmarium Lu 1-17
(FERM BP-1417) and riophyllum ulmarium M-8171 (FERM BP-1415) are used, artificial cultivation of each strain is carried out by the method described in the publication, and fruit bodies at an appropriate harvest time are harvested to obtain fruit bodies. A sensory test was performed. As the medium for artificial cultivation, 50 g of coniferous sawdust, 50 g of broadleaf sawdust and 90 g of rice bran described in the publication are used.
After mixing well with g and adjusting the water content to 65% with tap water, it was pressed into a polypropylene 850 ml wide-mouthed bottle and a hole with a diameter of 1 cm facing downward from the center of the bottle mouth was opened. 12 pieces of sawdust solid culture medium capped with a cap
What was subjected to high-pressure steam sterilization at 0 ° C. for 60 minutes was used as the A medium.
According to the method for preparing the medium, corncob meal 130
g, Mamekawa 60g, bran 30g were mixed in the same way, and B medium, 50g coniferous sawdust 50g, crushed cottonseed shell 50g, and 100g rice bran were similarly mixed to produce C medium, coniferous sawdust. A medium prepared in the same manner by thoroughly mixing 100 g and 70 g of Memekawa was used as D medium. Table 1 shows the yield (g / bin) of fruiting bodies and the result of the sensory test obtained in each medium used.

【０００７】[0007]

【表１】 表 １ ──────────────────────────────────── 使 用 菌 株 培 地 収量（ｇ／ビン） 官能検査 ──────────────────────────────────── リオフィラム ウルマリウム Ａ １１０ − Ｌｕ １−２ Ｂ １７８ ＋ Ｃ １４０ ＋ Ｄ １５３ ＋ リオフィラム ウルマリウム Ａ １１２ ＋＋ Ｌｕ １−８ Ｂ １７９ ＋＋＋ Ｃ １４２ ＋＋＋ Ｄ １５０ ＋＋＋ リオフィラム ウルマリウム Ａ １０８ − Ｌｕ １−１７ Ｂ １８０ ＋＋ Ｃ １４４ ＋ Ｄ １５５ ＋ リオフィラム ウルマリウム Ａ １１３ − Ｍ−８１７１ Ｂ １８８ ＋＋＋ Ｃ １５７ ＋＋＋ Ｄ １７１ ＋＋＋ ────────────────────────────────────[Table 1]                               Table 1 ────────────────────────────────────       Bacteria used Cultivated land Yield (g / bottle) Sensory test ──────────────────────────────────── Riofilum Ulmarium A 110-               Lu 1-2 B 178 +                                 C 140 +                                 D 153 + Riophyllum Ulmarium A 112 ++               Lu 1-8 B 179 +++                                 C 142 +++                                 D 150 +++ Riofilum Ulmarium A 108-             Lu 1-17 B 180 ++                                 C 144 +                                 D 155 + Riofilum Ulmarium A 113-               M-8171 B 188 +++                                 C 157 +++                                 D 171 +++ ────────────────────────────────────

【０００８】官能検査は、リオフィラム ウルマリウム
子実体を油炒めし、硫酸キニーネを、苦味の標準物質と
して、パネラーが判定する。油炒めは次の様に行う。す
なわち、得られたリオフィラム ウルマリウムの子実体
の柄の基部を除去し、１本ずつにわけ、軽く水洗いし
て、よく水をきる。次いで、フライパンにサラダ油５ｍ
ｌを加え、全体にのばし、中火で油を充分に熱する。前
述リオフィラム ウルマリウム子実体１００ｇ（湿重）
を加え、箸でかきまぜながら、２分間以上、全体に火を
通し、フライパンに水気が無くなれば、火を止める。該
子実体を常温になるまで冷まし、パネラーに苦くない、
苦い、かなり苦い、著しく苦いの四段階で評価させる。
なお、以下、各表中の−は苦くない、＋は苦い、＋＋は
かなり苦い、＋＋＋は著しく苦いを意味する。この＋、
−の中間がリオフィラム ウルマリウム子実体の苦味の
硫酸キニーネを標準物質とした苦味の閾値、すなわち、
ほとんど苦味を感じない苦味であり、標準物質の硫酸キ
ニーネの閾値、０．０００００８Ｍ（太田静行著、「食
品調味の知識」第２版第１刷、昭和６０年１０月１日、
幸書房発行、第３８頁）の苦味に相当する。前述の供試
菌株中、リオフィラム ウルマリウム Ｌｕ １−２が
最も低苦味菌株であるが、Ｂ、Ｃ、Ｄ培地を用いた場合
は、収量は増加するものの得られる子実体は苦味のある
ものとなる。In the sensory test, lyophilum ulmarium fruit bodies are stir-fried in oil, and quinine sulfate is used as a standard substance for bitterness by a panelist. Fried oil is performed as follows. That is, the base of the obtained fruit body of Riofilum ulmarium is removed, divided into individual pieces, lightly washed with water, and well drained. Next, add 5m of salad oil to the frying pan.
Add 1 liter, spread all over and heat oil well over medium heat. Riofilum ulmarium fruiting body 100g (wet weight)
Add the ingredients to the pan and stir with chopsticks for 2 minutes or more, and heat the whole mixture. When the pan is dry, turn off the heat. Allow the fruiting body to cool to room temperature and not bitter the panelist,
The four levels of bitterness, pretty bitterness, and remarkably bitterness are evaluated.
In the following, − in each table means no bitterness, + means bitterness, ++ means much bitterness, and ++ means remarkably bitterness. This +
The middle of − is the threshold of bitterness with quinine sulfate as a standard substance, which is the bitterness of Riofilum ulmarium fruiting body, that is,
It is a bitterness with almost no bitterness, the threshold value of quinine sulfate as a standard substance, 0.000008M (Shizuyuki Ota, "Knowledge of food seasoning" 2nd edition, 1st edition, October 1, 1985,
Corresponds to the bitterness of Koshou Shobo, page 38). Among the above-mentioned test strains, riophyllum ulmarium Lu 1-2 is the strain with the lowest bitterness, but when B, C or D medium is used, the yield increases but the fruiting body obtained has a bitter taste. .

【０００９】本発明において高苦味化培地とは、リオフ
ィラム ウルマリウム Ｌｕ １−２の人工栽培を行っ
た際に得られる子実体の苦味が官能検査により＋以上の
培地を意味し、上記Ｂ、Ｃ、Ｄ培地である。また本発明
の新菌株とは、該菌株を高苦味化培地で栽培して得られ
る子実体の苦味が、少なくとも一種の高苦味化培地につ
いて、苦味をほとんど感じない程度以下の菌株をいう。[0009] In the present invention the high bitterness of medium Lyophyllum ulmarium bitterness of fruit bodies obtained when performing the artificial cultivation of Lu 1-2 are means + or more media by sensory test, the upper Symbol B, C , D medium. Further, the new strain of the present invention refers to a strain in which the bitterness of fruiting bodies obtained by cultivating the strain in a high-bittering medium is such that the bitterness is hardly felt in at least one high-bittering medium.

【００１０】次に、本発明の新菌株の育種について述べ
る。 １．選抜育種 自然界に発生しているリオフィラム ウルマリウム１１
２個の子実体より組織分離を行い、純粋分離した菌糸体
９０菌株を得た。次にこの９０菌株を前記Ｂ培地を用い
栽培試験を行った。栽培試験により、子実体を形成した
菌株４８株の子実体を官能検査に供した。子実体の形状
に優れ、収量も多く、苦味の低い菌株１０株を選抜し
た。更にこの１０株をＢ、Ｃ、Ｄ培地で栽培試験し、工
業的栽培に適し、最も低苦味な菌株として、リオフィラ
ム ウルマリウム Ｌｕ １−１３を選抜した。Next, breeding of the new strain of the present invention will be described. 1. Selective breeding Riofilum ulmarium 11 occurring in the natural world
Tissue separation was carried out from the two fruiting bodies to obtain a purely separated mycelium 90 strain. Next, this 90 strain was subjected to a cultivation test using the B medium. By a cultivation test, fruit bodies of 48 strains that formed fruit bodies were subjected to a sensory test. Ten strains with excellent fruiting body shape, high yield, and low bitterness were selected. Further, these 10 strains were subjected to a cultivation test in B, C and D media, and Riofilum ulmarium Lu 1-13 was selected as a strain having the lowest bitterness and suitable for industrial cultivation.

【００１１】以下、このリオフィラム ウルマリウムに
ついて説明する。リオフィラム ウルマリウムＬｕ １
−１３株は、福島県裏磐梯にて枯れ木に叢生していた子
実体より本発明者らが組織分離したもので子実体及び胞
子の特徴は次のようである。子実体は叢生、カサは径５
〜１．５ｃｍ、円形又は不正形で丸山形、表面は平滑、
湿潤、白色〜帯褐クリーム色を呈しており、往々やや濃
色の斑紋を現し、老時中央にき製を生じることがある。
肉は白色、幅広く柄に上生する。柄は偏心性で湾曲し、
３〜７×１〜２ｃｍ、カサとほぼ同色、頂部は白色で綿
毛状ないし粉状である。胞子はほぼ球形、平滑、無色、
４．５〜５．５×３．５〜４．５μｍ、紋は白色であっ
た。以上の特徴を伊藤誠哉著「日本菌類誌」第二巻第五
号（１９５９年、養賢堂出版）の記載と比較すると、本
菌はリオフィラム ウルマリウムであることが明りょう
である。The riophyllum ulmarium will be described below. Riofilum Ulmarium Lu 1
The -13 strain was isolated by the present inventors from a fruit body that had been crowded on a dead tree in Urabandai, Fukushima Prefecture, and the characteristics of the fruit body and spores are as follows. Fruit bodies are crowded, and umbrellas are diameter 5
~ 1.5 cm, round or irregularly shaped round mountain, smooth surface,
It has a moist, white to brownish cream color, often presents a slightly dark mottle, and may cause squeezing in the middle of old age.
The flesh is white and has a wide variety of patterns. The handle is eccentric and curved,
3 to 7 x 1 to 2 cm, almost the same color as the bulk, white at the top, fluffy or powdery. Spores are almost spherical, smooth, colorless,
4.5 to 5.5 × 3.5 to 4.5 μm, and the pattern was white. Or more of the features of the Seiya Ito al., "Japan fungi magazine" the second volume fifth issue (195 nine years, nourishing Kashikodo publication) when compared with the description of, this bacterium is clearly to be a Lyophyllum ulmarium.

【００１２】次にリオフィラム ウルマリウム Ｌｕ
１−１３株の菌学的諸性質を示す。 （１）麦芽エキス寒天培地（２５℃培養） ７日目：旺盛な生育。コロニー径は４０mm。白色で密な
菌糸、気菌糸を多量に生じる。１０日目：シャーレ全体
に菌糸が生育する。１７日目：表面全体に密な気菌糸を
生じる。菌糸は白色。 （２）バレイショ・ブドウ糖寒天培地（２５℃培養） ７日目：旺盛な生育。コロニー径は３６mm。白色で密な
菌糸、気菌糸を多量に生じる。１０日目：シャーレ全体
に菌糸が生育する。１７日目：表面全体に密な気菌糸を
生じる。コロニー中央部は薄い黄色、他は白色。 （３）ツァペック・ドックス寒天培地（２５℃培養） ７日目：小程度の生育。コロニー径は２６mm。樹状に伸
長し極めて希薄な菌糸、気菌糸は少ない。１７日目：シ
ャーレ全体に菌糸が生育する。菌糸は樹状で希薄、白
色。 （４）サブロー寒天培地（２５℃培養） ７日目：旺盛な生育。コロニー径は４２mm。白色で綿状
の密な菌糸、気菌糸やや多い。１０日目：シャーレ全体
に菌糸が生育する。気菌糸を極めて多量に生じ、菌糸は
綿状で白色。 （５）オートミール寒天培地（２５℃培養） ７日目：旺盛な生育。コロニー径は３６mm。菌糸はよく
分枝して伸び、気菌糸は少ない。１０日目：シャーレ全
体に菌糸が生育する。綿状の気菌糸を多量に生じる。菌
糸は白色。 （６）合成ムコール寒天培地（２５℃培養） ７日目：小程度の生育。コロニー径は２１mm。菌糸は白
色で直線的に伸長し、放射状のコロニーを形成する。１
７日目：シャーレ全体に菌糸が生育する。気菌糸を多量
に生じる。菌糸は白色。 （７）ＹｐＳｓ寒天培地（２５℃培養） ７日目：旺盛な生育。コロニー径は４０mm。白色で密な
菌糸、気菌糸を多量に生じる。マット状。１０日目：シ
ャーレ全体に菌糸が生育する。密な気菌糸を多量に生じ
る。菌糸は白色だが、培地は黄色に変化する。 （８）フェノールオキシダーゼ検定用培地（２５℃培
養） ７日目：小程度の生育。コロニー径は１８mm。菌糸は白
色で短くマット状に生育、気菌糸は少ない。培地は褐
変、褐変半径は４０mm。１７日目：中程度の生育。コロ
ニー径は３７mm。褐変半径は４２mm。種菌の新旧により
著しく生育速度に差が生じる。 （９）最適生育温度 ＰＧＹ寒天培地に直径５mmの円盤状種菌を接種し、各温
度で１２日間培養した後、コロニー径を測定した。その
結果、最適な成育温度は２５℃付近であった。また、５
℃、３５℃ではほとんど生育しなかった。 (10) 最適生育ｐＨ ＰＧＹ液体培地（寒天を含まないＰＧＹ寒天培地）６０
mlずつを１００ml容三角フラスコに分注して殺菌し、酸
又はアルカリで各ｐＨに調整後に種菌を接種して、２５
℃、１５日間静置培養した後、菌体の乾燥重量を測定し
た。その結果、最適生育ｐＨは７〜８であった。また、
生育可能なｐＨ範囲は、ｐＨ３．５〜１０であった。Next, Riofilum ulmarium Lu
The various bacteriological properties of strains 1-13 are shown below. (1) Malt extract agar medium (25 ° C culture) Day 7: vigorous growth. The colony diameter is 40 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 10: Mycelia grow on the entire petri dish. Day 17: Dense aerial mycelia develop on the entire surface. Mycelia are white. (2) Potato-glucose agar medium (25 ° C. culture) Day 7: vigorous growth. The colony diameter is 36 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 10: Mycelia grow on the entire petri dish. Day 17: Dense aerial mycelia develop on the entire surface. Light yellow in the center of the colony, white in others. (3) Czapek-Dox agar medium (25 ° C. culture) Day 7: Small growth. The colony diameter is 26 mm. Very few mycelia and aerial hyphae that grow in a dendritic form and are extremely thin. Day 17: Mycelia grow on the entire petri dish. Mycelium is dendritic, thin and white. (4) Sabouraud agar medium (25 ° C. culture) Day 7: vigorous growth. The colony diameter is 42 mm. White, cotton-like dense hyphae, aerial hyphae. Day 10: Mycelia grow on the entire petri dish. An abundant amount of aerial mycelia is produced, and the hyphae are cotton-like and white. (5) Oatmeal agar medium (25 ° C. culture) Day 7: Vigorous growth. The colony diameter is 36 mm. Hyphae are well branched and elongated, and aerial hyphae are few. Day 10: Mycelia grow on the entire petri dish. A large amount of cotton-like aerial hyphae are produced. Mycelia are white. (6) Synthetic Mucor agar medium (25 ° C. culture) Day 7: Small growth. The colony diameter is 21 mm. The hyphae are white and linearly elongated, forming radial colonies. 1
Day 7: Mycelia grow on the entire dish. A large amount of aerial hyphae are produced. Mycelia are white. (7) YpSs agar medium (25 ° C. culture) Day 7: vigorous growth. The colony diameter is 40 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Matte shape. Day 10: Mycelia grow on the entire petri dish. A large amount of dense aerial hyphae are produced. The mycelium is white, but the medium turns yellow. (8) Phenol oxidase assay medium (25 ° C. culture) Day 7: Small growth. The colony diameter is 18 mm. Mycelium is white and grows short and mat-like with few aerial mycelia. The medium is browned and the radius of browning is 40 mm. Day 17: Medium growth. The colony diameter is 37 mm. The browning radius is 42 mm. The growth rate significantly varies depending on the old and new seeds. (9) Optimal growth temperature PGY agar medium was inoculated with a discoid inoculum having a diameter of 5 mm, cultured at each temperature for 12 days, and then the colony diameter was measured. As a result, the optimum growth temperature was around 25 ° C. Also, 5
Almost no growth occurred at 35 ° C and 35 ° C. (10) Optimal growth pH PGY liquid medium (PGY agar without agar) 60
Dispense each 100 ml into a 100 ml Erlenmeyer flask to sterilize, adjust each pH with acid or alkali, and inoculate inoculum, then
After static culture at ℃ for 15 days, the dry weight of the cells was measured. As a result, the optimum growth pH was 7-8. Also,
The viable pH range was pH 3.5 to 10.

【００１３】次に、リオフィラム ウルマリウム Ｌｕ
１−１３株と他のリオフィラムウルマリウムとの異同
判定として、両菌糸が持つ性因子が異なっていれば、そ
の菌糸は互いに異なる菌糸であるという菌類分類学的事
実に基づき、性因子の異同を寒天培地上における対峙培
養によって調べた。 供試したリオフィラム ウルマリウムとしてはリオフィ
ラム ウルマリウムＩＦＯ ９６３７、リオフィラム
ウルマリウム ＩＦＯ ３０５２５、リオフィラム ウ
ルマリウム ＩＦＯ ３０７７５、リオフィラム ウル
マリウム Ｌｕ １−２、リオフィラム ウルマリウム
Ｌｕ １−８、リオフィラム ウルマリウム Ｌｕ
１−１７、リオフィラム ウルマリウム Ｍ−８１７
１、リオフィラム ウルマリウム ＳＡである。なおリ
オフィラム ウルマリウム ＳＡは、市販のリオフィラ
ム ウルマリウム子実体よりの分離株である。 上記それぞれのリオフィラム ウルマリウムの二核菌糸
を保存スラントより３×３×３mmのブロックとして切り
出し、それぞれをＰＧＹ寒天培地の中央部に、リオフィ
ラム ウルマリウム Ｌｕ １−１３の二核菌糸と対峙
して植菌し（２cm間隔）、２５℃、１４日間培養後、両
菌株のコロニー間に帯線が生じるか否かを判定した。結
果を表２に示す。（帯線を生じた場合＋、生じなかった
場合−）。Next, Riofilum ulmarium Lu
Based on the taxonomic fact that the hyphae are different hyphae, if the sexes possessed by both hyphae are different from each other, the sex Differences were examined by inversion culture on agar. The tested Riofilum ulmarium is Riofilum ulmarium IFO 9637, Riofilum
Ulmarium IFO 30525, Riofilum Ulmarium IFO 30775, Riofilum Ulmarium Lu 1-2, Riofilum Ulmarium Lu 1-8, Riofilum Ulmarium Lu
1-17, Riofilum Ulmarium M-817
1. Riofilum ulmarium SA. It should be noted that Riofilum ulmarium SA is an isolated strain from the commercially available Riofilum ulmarium fruiting body. Each of the above-mentioned binuclear hyphae of riophyllum ulmarium was cut out from the preserved slant as a block of 3 × 3 × 3 mm, and each was inoculated in the center of the PGY agar medium so as to confront the binuclear hyphae of riophyllum ulmarium Lu 1-13. After culturing (at 2 cm intervals) at 25 ° C. for 14 days, it was determined whether a band line was formed between colonies of both strains. The results are shown in Table 2. (+ If banding occurred, -if not).

【００１４】[0014]

【表２】 表 ２ ───────────────────────────────── リオフィラム ウルマリウム ＩＦＯ ９６３７ ＋ ＩＦＯ ３０５２５ ＋ ＩＦＯ ３０７７５ ＋ Ｌｕ １−２ ＋ Ｌｕ １−８ ＋ Ｌｕ １−１７ ＋ Ｍ−８１７１ ＋ ＳＡ ＋ ─────────────────────────────────[Table 2]                             Table 2   ──────────────────────────────────     Riophyllum Ulmarium IFO 9637 +                                 IFO 30525 +                                 IFO 30775 +                                 Lu 1-2 +                                 Lu 1-8 +                                 Lu 1-17 +                                 M-8171 +                                 SA +   ──────────────────────────────────

【００１５】表２よりわかるように、前記各菌株は、リ
オフィラム ウルマリウム Ｌｕ１−１３との対峙培養
ですべて帯線を生じ、このことからリオフィラム ウル
マリウム Ｌｕ １−１３は新しい菌株であることは明
白である。以上説明したように本発明の選抜育種による
新菌株として、例えばリオフィラム ウルマリウム Ｌ
ｕ １−１３が挙げられるが、前記菌株と同様に高苦味
化培地で栽培して得られる子実体の苦味が、少なくとも
一種の高苦味化培地について、苦味をほとんど感じない
程度以下であるという特性を示す菌株は、すべて本発明
に属するものである。As can be seen from Table 2, all of the above-mentioned strains produced zonal lines in the confrontation culture with Riophyllum ulmarium Lu1-13, and it is clear that Riofilum ulmarium Lu1-13 is a new strain. . As described above, as a new strain by selective breeding of the present invention, for example, Riofilum ulmarium L
u 1-13 can be mentioned, but the bitterness of the fruiting body obtained by cultivating in a high bittering medium as in the case of the strain is such that, for at least one type of high bittering medium, the bitterness is less than or equal to a level at which little bitterness is felt. The strains shown in are all belonging to the present invention.

【００１６】２．交配育種 本発明の新菌株を交配育種により得た。特開昭６３−２
７３４６７号公報に記載のように、リオフィラム ウル
マリウム Ｌｕ １−８とリオフィラム ウルマリウム
Ｌｕ １−１７の交配により、収量、生育速度、子実
体形態に優れたリオフィラム ウルマリウム Ｍ−８１
７１が得られている。栽培特性に優れ、かつ子実体の苦
味が低減された菌株を交配育種するために、このリオフ
ィラム ウルマリウム Ｌｕ １−８、リオフィラム
ウルマリウム Ｌｕ １−１７の両菌株を親株として用
いた。リオフィラム ウルマリウム Ｌｕ １−８とリ
オフィラム ウルマリウムＬｕ １−１７をＡ培地にそ
れぞれ生育させ、通常の操作により子実体を発生させ
た。該子実体よりそれぞれ胞子を回収し、発芽させた一
核菌糸を単離して、５７株ずつを総当り交配して１６５
３株を得た。この１６５３株をＢ培地を用いて栽培試験
を行い、官能検査により低苦味な菌株８０株を選択し、
次にＢ、Ｃ、Ｄ培地で栽培試験を行い、栽培特性に優
れ、苦味が、少なくとも一種の高苦味化培地について、
苦味をほとんど感じない程度以下である８菌株を選抜
し、それぞれリオフィラム ウルマリウム Ｋ−０４２
９、リオフィラム ウルマリウム Ｋ−０２０７、リオ
フィラム ウルマリウム Ｋ−０２０２、リオフィラム
ウルマリウム Ｋ−０２５９、リオフィラム ウルマ
リウム Ｋ−１００４、リオフィラム ウルマリウム
Ｋ−１２５７、リオフィラム ウルマリウム Ｋ−６８
０４、リオフィラム ウルマリウム Ｋ−６８０６とそ
れぞれ命名した。2. Cross Breeding The new strain of the invention was obtained by cross breeding. JP-A-63-2
As described in Japanese Patent Publication No. 73467, by crossing Riofilum ulmarium Lu 1-8 with Riofilum ulmarium Lu 1-17, Riofilum ulmarium M-81 excellent in yield, growth rate and fruiting body morphology.
71 has been obtained. In order to cross-bred strains having excellent cultivation characteristics and reduced bitterness of fruiting bodies, this riophyllum ulmarium lu 1-8, riophyllum
Both strains of Ulmarium Lu 1-17 were used as parent strains. Riofilum ulmarium Lu 1-8 and riophyllum ulmarium Lu 1-17 were grown in A medium, respectively, and fruiting bodies were generated by a normal operation. Spores were collected from each of the fruiting bodies, germinated mononuclear hyphae were isolated, and 57 strains were bred for 165 rounds.
3 strains were obtained. This 1653 strain was subjected to a cultivation test using B medium, and 80 strains with low bitterness were selected by a sensory test,
Next, a cultivation test was carried out in B, C, and D media, and the cultivation characteristics were excellent, and the bitterness was at least one of the high-bitterness media
Eight strains having almost no bitterness were selected, and lyophilum ulmarium K-042 was selected.
9, Riofilum ulmarium K-0207, Riofilum ulmarium K-0202, Riofilum ulmarium K-0259, Riofilum ulmarium K-1004, Riofilum ulmarium
K-1257, Riofilum ulmarium K-68
04, and Riofilum ulmarium K-6806, respectively.

【００１７】次にこのリオフィラム ウルマリウム Ｋ
−０４２９株の菌学的諸性質を示す。 （１）麦芽エキス寒天培地（２５℃培養） ５日目：コロニー径は１８mm。白色で密な菌糸、気菌糸
を多量に生じる。１０日目：コロニー径は４７mm。２０
日目：コロニー径は８３mm。放射状に生育して表面全体
に密な気菌糸を生じる。菌糸は白色。 （２）バレイショ・ブドウ糖寒天培地（２５℃培養） １０日目：中程度の生育。コロニー径は４０mm。白色で
密な菌糸、気菌糸を多量に生じる。１５日目：コロニー
径は５５mm。２０日目：コロニー径は６６mm。表面全体
に密な気菌糸を生じる。綿状のコロニーで、菌糸は白
色。 （３）ツァペック・ドックス寒天培地（２５℃培養） １０日目：小程度の生育。コロニー径は２８mm。樹状に
伸長し極めて希薄な菌糸、気菌糸は少ない。２０日目：
コロニー径は５２mm。菌糸は樹状で希薄、白色。 （４）サブロー寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は５０mm。白色で綿
状の密な菌糸、気菌糸やや多い。２０日目：シャーレ全
体に菌糸が生育する。放射状に生育して表面全体に気菌
糸を生じる。菌糸は白色。 （５）オートミール寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は５１mm。綿状のコ
ロニーで、気菌糸を生じる。２０日目：シャーレ全体に
菌糸が生育する。綿状のコロニーで、気菌糸を多量に生
じる。菌糸は白色。 （６）合成ムコール寒天培地（２５℃培養） １０日目：小程度の生育。コロニー径は２７mm。菌糸は
白色で、樹枝状のコロニーを形成する。２０日目：コロ
ニー径は４８mm。気菌糸を生じる。樹枝状のコロニー
で、菌糸は白色。 （７）ＹｐＳｓ寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は４９mm。綿状のコ
ロニーで白色な菌糸、気菌糸を生じる。２０日目：シャ
ーレ全体に菌糸が生育する。密な気菌糸を多量に生じ
る。菌糸は白色。 （８）フェノールオキシダーゼ検定用培地（２５℃培
養） ５日目：小程度の生育。コロニー径は１８mm。菌糸は白
色で気菌糸を多量に生じる。培地は褐変して、褐変半径
は３４mm。１５日目：旺盛な生育。コロニー径は６４m
m。培地全面が褐変。種菌の新旧により著しく生育速度
に差が生じる。 （９）最適生育温度 ＰＧＹ寒天培地に直径５mmの円盤状種菌を接種し、各温
度で１２日間培養した後、コロニー径を測定した。その
結果、最適な成育温度は２５℃付近であった。また、５
℃、３５℃ではほとんど生育しなかった。 (10) 最適生育ｐＨ ＰＧＹ液体培地（寒天を含まないＰＧＹ寒天培地）６０
mlずつを１００ml容三角フラスコに分注して殺菌し、酸
又はアルカリで各ｐＨに調整後に種菌を接種して、２５
℃、１５日間静置培養した後、菌体の乾燥重量を測定し
た。その結果、最適生育ｐＨは７〜８であった。また、
生育可能なｐＨ範囲は、ｐＨ３．５〜１０であった。Next, this Riofilum ulmarium K
The bacteriological properties of the -0429 strain are shown. (1) Malt extract agar medium (25 ° C. culture) Day 5: Colony diameter 18 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 10: Colony diameter 47 mm. 20
Day: Colony diameter is 83 mm. It grows radially and produces dense aerial hyphae on the entire surface. Mycelia are white. (2) Potato-glucose agar medium (25 ° C. culture) Day 10: Medium growth. The colony diameter is 40 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 15: Colony diameter 55 mm. Day 20: Colony diameter 66 mm. Dense aerial hyphae are produced on the entire surface. Cotton-like colony with white hyphae. (3) Czapek-Dox agar medium (25 ° C. culture) Day 10: Small growth. The colony diameter is 28 mm. Very few mycelia and aerial hyphae that grow in a dendritic form and are extremely thin. Day 20:
The colony diameter is 52 mm. Mycelium is dendritic, thin and white. (4) Sabouraud agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 50 mm. White, cotton-like dense hyphae, aerial hyphae. Day 20: Mycelia grow on the entire dish. It grows radially and produces aerial mycelia on the entire surface. Mycelia are white. (5) Oatmeal agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 51 mm. A flocculent colony that produces aerial hyphae. Day 20: Mycelia grow on the entire dish. A flocculent colony with a large amount of aerial hyphae. Mycelia are white. (6) Synthetic Mucor agar medium (25 ° C. culture) Day 10: Small growth. The colony diameter is 27 mm. The hyphae are white and form dendritic colonies. 20th day: Colony diameter is 48 mm. Produces aerial mycelia. Dendritic colony with white hyphae. (7) YpSs agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 49 mm. Fluffy colonies produce white hyphae and aerial hyphae. Day 20: Mycelia grow on the entire dish. A large amount of dense aerial hyphae are produced. Mycelia are white. (8) Phenol oxidase assay medium (25 ° C. culture) Day 5: Small growth. The colony diameter is 18 mm. Mycelium is white and produces a large amount of aerial mycelium. The medium turned brown and the browning radius was 34 mm. Day 15: Strong growth. Colony diameter is 64m
m. The entire surface of the medium turns brown. The growth rate significantly varies depending on the old and new seeds. (9) Optimal growth temperature PGY agar medium was inoculated with a discoid inoculum having a diameter of 5 mm, cultured at each temperature for 12 days, and then the colony diameter was measured. As a result, the optimum growth temperature was around 25 ° C. Also, 5
Almost no growth occurred at 35 ° C and 35 ° C. (10) Optimal growth pH PGY liquid medium (PGY agar without agar) 60
Dispense each 100 ml into a 100 ml Erlenmeyer flask to sterilize, adjust each pH with acid or alkali, and inoculate inoculum, then
After static culture at ℃ for 15 days, the dry weight of the cells was measured. As a result, the optimum growth pH was 7-8. Also,
The viable pH range was pH 3.5 to 10.

【００１８】次にリオフィラム ウルマリウム Ｋ−０
２０７株の菌学的諸性質を示す。 （１）麦芽エキス寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は６１mm。白色で放
射状のコロニー、気菌糸を少量生じる。２０日目：シャ
ーレ全体に菌糸が生育する。気菌糸は少ない。菌糸は白
色。 （２）バレイショ・ブドウ糖寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は５３mm。白色で密
な菌糸、気菌糸を多量に生じる。２０日目：シャーレ全
体に菌糸が生育する。表面全体に密な気菌糸を生じる。
コロニーは綿状で白色。 （３）ツァペック・ドックス寒天培地（２５℃培養） ７日目：旺盛な生育。コロニー径は５１mm。樹状に伸長
し極めて希薄な菌糸、気菌糸は少ない。１５日目：コロ
ニー径は７５mm。菌糸は樹状で希薄、白色。２０日目：
コロニー径は７７mm。 （４）サブロー寒天培地（２５℃培養） １０日目：とても旺盛な生育。コロニー径は５８mm。白
色で放射状の密な菌糸、気菌糸やや多い。１５日目：シ
ャーレ全体に菌糸が生育する。気菌糸を生じ、菌糸は放
射状で白色。 （５）オートミール寒天培地（２５℃培養） １０日目：極めて旺盛な生育。コロニー径は６６mm。コ
ロニーは綿状で、気菌糸を生じる。１５日目：シャーレ
全体に菌糸が生育する。綿状のコロニーで気菌糸を生じ
る。菌糸は白色。 （６）合成ムコール寒天培地（２５℃培養） １０日目：中程度の生育。コロニー径は３３mm。菌糸は
白色でやや薄く、樹枝状に伸長し、気菌糸を伴う。２０
日目：コロニー径は７４mm。コロニーは希薄で樹枝状、
菌糸は白色。 （７）ＹｐＳｓ寒天培地（２５℃培養） １０日目：極めて旺盛な生育。コロニー径は６５mm。白
色で密な菌糸、気菌糸を多量に生じる。１５日目：シャ
ーレ全体に菌糸が生育する。密な気菌糸を多量に生じ
る。菌糸は白色。 （８）フェノールオキシダーゼ検定用培地（２５℃培
養） １０日目：旺盛な生育。コロニー径は６３mm。菌糸は白
色で、気菌糸を多量に生じる。培地は褐変、褐変半径は
６５mm。１５日目：シャーレ全体に菌糸が生育する。気
菌糸を多量に生じ、培地全面が褐変する。種菌の新旧に
より著しく生育速度に差が生じる。 （９）最適生育温度 ＰＧＹ寒天培地に直径５mmの円盤状種菌を接種し、各温
度で１２日間培養した後、コロニー径を測定した。その
結果、最適な成育温度は２５℃付近であった。また、５
℃、３５℃ではほとんど生育しなかった。 (10) 最適生育ｐＨ ＰＧＹ液体培地（寒天を含まないＰＧＹ寒天培地）６０
mlずつを１００ml容三角フラスコに分注して殺菌し、酸
又はアルカリで各ｐＨに調整後に種菌を接種して、２５
℃、１５日間静置培養した後、菌体の乾燥重量を測定し
た。その結果、最適生育ｐＨは７〜８であった。また、
生育可能なｐＨ範囲は、ｐＨ３．５〜１０であった。Next, Riofilum ulmarium K-0
The mycological properties of strain 207 are shown. (1) Malt extract agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 61 mm. White, radial colonies, small amount of aerial mycelia. Day 20: Mycelia grow on the entire dish. There are few aerial hyphae. Mycelia are white. (2) Potato-glucose agar medium (25 ° C. culture) Day 10: Vigorous growth. The colony diameter is 53 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 20: Mycelia grow on the entire dish. Dense aerial hyphae are produced on the entire surface.
The colony is cotton and white. (3) Czapek-Dox agar medium (25 ° C. culture) Day 7: vigorous growth. The colony diameter is 51 mm. Very few mycelia and aerial hyphae that grow in a dendritic form and are extremely thin. Day 15: Colony diameter 75 mm. Mycelium is dendritic, thin and white. Day 20:
The colony diameter is 77 mm. (4) Sabouraud agar medium (25 ° C. culture) Day 10: Very vigorous growth. The colony diameter is 58 mm. White, radial dense hyphae, aerial hyphae. Day 15: Mycelia grow on the entire dish. Aerial hyphae are produced, and the hyphae are radial and white. (5) Oatmeal agar medium (25 ° C. culture) Day 10: Very vigorous growth. The colony diameter is 66 mm. Colonies are flocculent and produce aerial hyphae. Day 15: Mycelia grow on the entire dish. Flocky colonies produce aerial hyphae. Mycelia are white. (6) Synthetic Mucor agar medium (25 ° C. culture) Day 10: Medium growth. The colony diameter is 33 mm. Hyphae are white and slightly thin, dendritic, with aerial hyphae. 20
Day: Colony diameter is 74 mm. The colony is thin and dendritic,
Mycelia are white. (7) YpSs agar medium (25 ° C. culture) Day 10: Very vigorous growth. The colony diameter is 65 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 15: Mycelia grow on the entire dish. A large amount of dense aerial hyphae are produced. Mycelia are white. (8) Phenol oxidase assay medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 63 mm. Mycelium is white and produces a large amount of aerial mycelia. The medium is browned and the radius of browning is 65 mm. Day 15: Mycelia grow on the entire dish. A large amount of aerial mycelia are produced, and the entire surface of the medium turns brown. The growth rate significantly varies depending on the old and new seeds. (9) Optimal growth temperature PGY agar medium was inoculated with a discoid inoculum having a diameter of 5 mm, cultured at each temperature for 12 days, and then the colony diameter was measured. As a result, the optimum growth temperature was around 25 ° C. Also, 5
Almost no growth occurred at 35 ° C and 35 ° C. (10) Optimal growth pH PGY liquid medium (PGY agar without agar) 60
Dispense each 100 ml into a 100 ml Erlenmeyer flask to sterilize, adjust each pH with acid or alkali, and inoculate inoculum, then
After static culture at ℃ for 15 days, the dry weight of the cells was measured. As a result, the optimum growth pH was 7-8. Also,
The viable pH range was pH 3.5 to 10.

【００１９】次にリオフィラム ウルマリウム Ｋ−０
２０２株の菌学的諸性質を示す。 （１）麦芽エキス寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は６０mm。白色で放
射状のコロニー、気菌糸を少量生じる。２０日目：シャ
ーレ全体に菌糸が生育する。気菌糸は少ない。菌糸は白
色。 （２）バレイショ・ブドウ糖寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は５９mm。白色で密
な菌糸、気菌糸を多量に生じる。２０日目：シャーレ全
体に菌糸が生育する。表面全体に密な気菌糸を生じる。
コロニーは綿状で白色。 （３）ツァペック・ドックス寒天培地（２５℃培養） ５日目：旺盛な生育。コロニー径は１６mm。樹状に伸長
し極めて希薄な菌糸、気菌糸は少ない。１５日目：コロ
ニー径は６２mm。菌糸は樹状で希薄、白色。２０日目：
コロニー径は７７mm。 （４）サブロー寒天培地（２５℃培養） １０日目：とても旺盛な生育。コロニー径は６６mm。白
色で放射状の密な菌糸、気菌糸やや多い。１５日目：シ
ャーレ全体に菌糸が生育する。気菌糸を生じ、菌糸は放
射状で白色。 （５）オートミール寒天培地（２５℃培養） １０日目：極めて旺盛な生育。コロニー径は６８mm。コ
ロニーは綿状で、気菌糸を生じる。１５日目：シャーレ
全体に菌糸が生育する。綿状のコロニーで気菌糸を生じ
る。菌糸は白色。 （６）合成ムコール寒天培地（２５℃培養） １０日目：中程度の生育。コロニー径は３３mm。菌糸は
白色でやや薄く、樹枝状に伸長し、気菌糸を伴う。２０
日目：コロニー径は６２mm。コロニーは希薄で樹枝状、
菌糸は白色。 （７）ＹｐＳｓ寒天培地（２５℃培養） １０日目：極めて旺盛な生育。コロニー径は６８mm。白
色で密な菌糸、気菌糸を多量に生じる。１５日目：シャ
ーレ全体に菌糸が生育する。密な気菌糸を多量に生じ
る。菌糸は白色。 （８）フェノールオキシダーゼ検定用培地（２５℃培
養） １０日目：旺盛な生育。コロニー径は６１mm。菌糸は白
色で、気菌糸を多量に生じる。培地は褐変。１５日目：
シャーレ全体に菌糸が生育する。気菌糸を多量に生じ、
培地全面が褐変する。種菌の新旧により著しく生育速度
に差が生じる。 （９）最適生育温度 ＰＧＹ寒天培地に直径５mmの円盤状種菌を接種し、各温
度で１２日間培養した後、コロニー径を測定した。その
結果、最適な成育温度は２５℃付近であった。また、５
℃、３５℃ではほとんど生育しなかった。 (10) 最適生育ｐＨ ＰＧＹ液体培地（寒天を含まないＰＧＹ寒天培地）６０
mlずつを１００ml容三角フラスコに分注して殺菌し、酸
又はアルカリで各ｐＨに調整後に種菌を接種して、２５
℃、１５日間静置培養した後、菌体の乾燥重量を測定し
た。その結果、最適生育ｐＨは７〜８．５であった。ま
た、生育可能なｐＨ範囲は、ｐＨ３．５〜１０であっ
た。Next, Riofilum ulmarium K-0
The mycological properties of the 202 strain are shown. (1) Malt extract agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 60 mm. White, radial colonies, small amount of aerial mycelia. Day 20: Mycelia grow on the entire dish. There are few aerial hyphae. Mycelia are white. (2) Potato-glucose agar medium (25 ° C. culture) Day 10: Vigorous growth. The colony diameter is 59 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 20: Mycelia grow on the entire dish. Dense aerial hyphae are produced on the entire surface.
The colony is cotton and white. (3) Czapek-Dox agar medium (25 ° C. culture) Day 5: vigorous growth. The colony diameter is 16 mm. Very few mycelia and aerial hyphae that grow in a dendritic form and are extremely thin. Day 15: Colony diameter is 62 mm. Mycelium is dendritic, thin and white. Day 20:
The colony diameter is 77 mm. (4) Sabouraud agar medium (25 ° C. culture) Day 10: Very vigorous growth. The colony diameter is 66 mm. White, radial dense hyphae, aerial hyphae. Day 15: Mycelia grow on the entire dish. Aerial hyphae are produced, and the hyphae are radial and white. (5) Oatmeal agar medium (25 ° C. culture) Day 10: Very vigorous growth. The colony diameter is 68 mm. Colonies are flocculent and produce aerial hyphae. Day 15: Mycelia grow on the entire dish. Flocky colonies produce aerial hyphae. Mycelia are white. (6) Synthetic Mucor agar medium (25 ° C. culture) Day 10: Medium growth. The colony diameter is 33 mm. Hyphae are white and slightly thin, dendritic, with aerial hyphae. 20
Day: Colony diameter is 62 mm. The colony is thin and dendritic,
Mycelia are white. (7) YpSs agar medium (25 ° C. culture) Day 10: Very vigorous growth. The colony diameter is 68 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 15: Mycelia grow on the entire dish. A large amount of dense aerial hyphae are produced. Mycelia are white. (8) Phenol oxidase assay medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 61 mm. Mycelium is white and produces a large amount of aerial mycelia. The medium turned brown. Day 15:
Mycelia grow on the entire dish. A large amount of aerial hyphae,
The entire surface of the medium turns brown. The growth rate significantly varies depending on the old and new seeds. (9) Optimal growth temperature PGY agar medium was inoculated with a discoid inoculum having a diameter of 5 mm, cultured at each temperature for 12 days, and then the colony diameter was measured. As a result, the optimum growth temperature was around 25 ° C. Also, 5
Almost no growth occurred at 35 ° C and 35 ° C. (10) Optimal growth pH PGY liquid medium (PGY agar without agar) 60
Dispense each 100 ml into a 100 ml Erlenmeyer flask to sterilize, adjust each pH with acid or alkali, and inoculate inoculum, then
After static culture at ℃ for 15 days, the dry weight of the cells was measured. As a result, the optimum growth pH was 7 to 8.5. Moreover, the pH range in which growth was possible was pH 3.5 to 10.

【００２０】次にこのリオフィラム ウルマリウム Ｋ
−０２５９株の菌学的諸性質を示す。 （１）麦芽エキス寒天培地（２５℃培養） ５日目：コロニー径は２４mm。白色で密な菌糸、気菌糸
を多量生じる。１０日目：コロニー径は６４mm。１５日
目：シャーレ全体に菌糸が生育する。放射状に生育して
表面全体に密な気菌糸を生じる。菌糸は白色。 （２）バレイショ・ブドウ糖寒天培地（２５℃培養） １０日目：中程度の生育。コロニー径は６１mm。白色で
密な菌糸、気菌糸を多量に生じる。１５日目：コロニー
径は８５mm。２０日目：シャーレ全体に菌糸が生育す
る。表面全体に密な気菌糸を生じる。綿状のコロニー
で、菌糸は白色。 （３）ツァペック・ドックス寒天培地（２５℃培養） １０日目：小程度の生育。コロニー径は４８mm。樹状に
伸長し極めて希薄な菌糸、気菌糸は少ない。２０日目：
コロニー径は７５mm。菌糸は樹状で希薄、白色。 （４）サブロー寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は６５mm。白色で綿
状の密な菌糸、気菌糸やや多い。２０日目：シャーレ全
体に菌糸が生育する。放射状に生育して表面全体に気菌
糸を生じる。菌糸は白色。 （５）オートミール寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は６７mm。綿状のコ
ロニーで、気菌糸を生じる。２０日目：シャーレ全体に
菌糸が生育する。綿状のコロニーで、気菌糸を多量に生
じる。菌糸は白色。 （６）合成ムコール寒天培地（２５℃培養） １０日目：小程度の生育。コロニー径は３９mm。菌糸は
白色で、樹枝状のコロニーを形成する。２０日目：コロ
ニー径は６６mm。気菌糸を生じる。樹枝状のコロニー
で、菌糸は白色。 （７）ＹｐＳｓ寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は６３mm。綿状のコ
ロニーで白色な菌糸、気菌糸を生じる。２０日目：シャ
ーレ全体に菌糸が生育する。密な気菌糸を多量に生じ
る。菌糸は白色。 （８）フェノールオキシダーゼ検定用培地（２５℃培
養） ５日目：小程度の生育。コロニー径は２９mm。菌糸は白
色で気菌糸を多量に生じる。培地は褐変。１５日目：旺
盛な生育。コロニー径は８８mm。培地全面が褐変。種菌
の新旧により著しく生育速度に差が生じる。 （９）最適生育温度 ＰＧＹ寒天培地に直径５mmの円盤状種菌を接種し、各温
度で１２日間培養した後、コロニー径を測定した。その
結果、最適な成育温度は２５℃付近であった。また、５
℃、３５℃ではほとんど生育しなかった。 (10) 最適生育ｐＨ ＰＧＹ液体培地（寒天を含まないＰＧＹ寒天培地）６０
mlずつを１００ml容三角フラスコに分注して殺菌し、酸
又はアルカリで各ｐＨに調整後に種菌を接種して、２５
℃、１５日間静置培養した後、菌体の乾燥重量を測定し
た。その結果、最適生育ｐＨは６〜８であった。また、
生育可能なｐＨ範囲は、ｐＨ３．５〜１０であった。Next, this Riofilum ulmarium K
The mycological properties of the -0259 strain are shown. (1) Malt extract agar medium (25 ° C. culture) Day 5: Colony diameter 24 mm. A large amount of white and dense mycelia and aerial mycelia are produced. Day 10: Colony diameter 64 mm. Day 15: Mycelia grow on the entire dish. It grows radially and produces dense aerial hyphae on the entire surface. Mycelia are white. (2) Potato-glucose agar medium (25 ° C. culture) Day 10: Medium growth. The colony diameter is 61 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 15: 85 mm colony diameter. Day 20: Mycelia grow on the entire dish. Dense aerial hyphae are produced on the entire surface. Cotton-like colony with white hyphae. (3) Czapek-Dox agar medium (25 ° C. culture) Day 10: Small growth. The colony diameter is 48 mm. Very few mycelia and aerial hyphae that grow in a dendritic form and are extremely thin. Day 20:
The colony diameter is 75 mm. Mycelium is dendritic, thin and white. (4) Sabouraud agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 65 mm. White, cotton-like dense hyphae, aerial hyphae. Day 20: Mycelia grow on the entire dish. It grows radially and produces aerial mycelia on the entire surface. Mycelia are white. (5) Oatmeal agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 67 mm. A flocculent colony that produces aerial hyphae. Day 20: Mycelia grow on the entire dish. A flocculent colony with a large amount of aerial hyphae. Mycelia are white. (6) Synthetic Mucor agar medium (25 ° C. culture) Day 10: Small growth. The colony diameter is 39 mm. The hyphae are white and form dendritic colonies. Day 20: Colony diameter 66 mm. Produces aerial mycelia. Dendritic colony with white hyphae. (7) YpSs agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 63 mm. Fluffy colonies produce white hyphae and aerial hyphae. Day 20: Mycelia grow on the entire dish. A large amount of dense aerial hyphae are produced. Mycelia are white. (8) Phenol oxidase assay medium (25 ° C. culture) Day 5: Small growth. The colony diameter is 29 mm. Mycelium is white and produces a large amount of aerial mycelium. The medium turned brown. Day 15: Strong growth. The colony diameter is 88 mm. The entire surface of the medium turns brown. The growth rate significantly varies depending on the old and new seeds. (9) Optimal growth temperature PGY agar medium was inoculated with a discoid inoculum having a diameter of 5 mm, cultured at each temperature for 12 days, and then the colony diameter was measured. As a result, the optimum growth temperature was around 25 ° C. Also, 5
Almost no growth occurred at 35 ° C and 35 ° C. (10) Optimal growth pH PGY liquid medium (PGY agar without agar) 60
Dispense each 100 ml into a 100 ml Erlenmeyer flask to sterilize, adjust each pH with acid or alkali, and inoculate inoculum, then
After static culture at ℃ for 15 days, the dry weight of the cells was measured. As a result, the optimum growth pH was 6-8. Also,
The viable pH range was pH 3.5 to 10.

【００２１】次にリオフィラム ウルマリウム Ｋ−１
００４株の菌学的諸性質を示す。 （１）麦芽エキス寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は６４mm。白色で放
射状のコロニー、気菌糸を少量生じる。２０日目：シャ
ーレ全体に菌糸が生育する。気菌糸は少ない。菌糸は白
色。 （２）バレイショ・ブドウ糖寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は６４mm。白色で密
な菌糸、気菌糸を多量に生じる。２０日目：シャーレ全
体に菌糸が生育する。表面全体に密な気菌糸を生じる。
コロニーは綿状で白色。 （３）ツァペック・ドックス寒天培地（２５℃培養） ５日目：旺盛な生育。コロニー径は１４mm。樹状に伸長
し極めて希薄な菌糸、気菌糸は少ない。１５日目：コロ
ニー径は６０mm。菌糸は樹状で希薄、白色。２０日目：
コロニー径は７５mm。 （４）サブロー寒天培地（２５℃培養） １０日目：とても旺盛な生育。コロニー径は８３mm。白
色で放射状の密な菌糸、気菌糸やや多い。１５日目：シ
ャーレ全体に菌糸が生育する。気菌糸を生じ、菌糸は放
射状で白色。 （５）オートミール寒天培地（２５℃培養） １０日目：極めて旺盛な生育。コロニー径は８１mm。コ
ロニーは綿状で、多量の気菌糸を生じる。１５日目：シ
ャーレ全体に菌糸が生育する。綿状のコロニーで気菌糸
を生じる。菌糸は白色。 （６）合成ムコール寒天培地（２５℃培養） １０日目：中程度の生育。コロニー径は２３mm。菌糸は
白色でやや薄く、樹枝状に伸張し、気菌糸を伴う。２０
日目：コロニー径は４５mm。コロニーは希薄で樹枝状。
菌糸は白色。 （７）ＹｐＳｓ寒天培地（２５℃培養） １０日目：極めて旺盛な生育。コロニー径は８０mm。白
色で密な菌糸、気菌糸を多量に生じる。１５日目：シャ
ーレ全体に菌糸が生育する。密な気菌糸を多量に生じ
る。菌糸は白色。 （８）フェノールオキシダーゼ検定用培地（２５℃培
養） １０日目：旺盛な生育。コロニー径は６８mm。菌糸は白
色で、気菌糸を多量に生じる。培地は褐変。１５日目：
シャーレ全体に菌糸が生育する。気菌糸を多量に生じ、
培地全面が褐変する。種菌の新旧により著しく生育速度
に差が生じる。 （９）最適生育温度 ＰＧＹ寒天培地に直径５mmの円盤状種菌を接種し、各温
度で１２日間培養した後、コロニー径を測定した。その
結果、最適な成育温度は２５℃付近であった。また、５
℃、３５℃ではほとんど生育しなかった。 (10) 最適生育ｐＨ ＰＧＹ液体培地（寒天を含まないＰＧＹ寒天培地）６０
mlずつを１００ml容三角フラスコに分注して殺菌し、酸
又はアルカリで各ｐＨに調整後に種菌を接種して、２５
℃、１５日間静置培養した後、菌体の乾燥重量を測定し
た。その結果、最適生育ｐＨは６〜７であった。また、
生育可能なｐＨ範囲は、ｐＨ３．５〜１０であった。Next, Riofilum ulmarium K-1
The mycological properties of strain 004 are shown below. (1) Malt extract agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 64 mm. White, radial colonies, small amount of aerial mycelia. Day 20: Mycelia grow on the entire dish. There are few aerial hyphae. Mycelia are white. (2) Potato-glucose agar medium (25 ° C. culture) Day 10: Vigorous growth. The colony diameter is 64 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 20: Mycelia grow on the entire dish. Dense aerial hyphae are produced on the entire surface.
The colony is cotton and white. (3) Czapek-Dox agar medium (25 ° C. culture) Day 5: vigorous growth. The colony diameter is 14 mm. Very few mycelia and aerial hyphae that grow in a dendritic form and are extremely thin. Day 15: Colony diameter 60 mm. Mycelium is dendritic, thin and white. Day 20:
The colony diameter is 75 mm. (4) Sabouraud agar medium (25 ° C. culture) Day 10: Very vigorous growth. The colony diameter is 83 mm. White, radial dense hyphae, aerial hyphae. Day 15: Mycelia grow on the entire dish. Aerial hyphae are produced, and the hyphae are radial and white. (5) Oatmeal agar medium (25 ° C. culture) Day 10: Very vigorous growth. The colony diameter is 81 mm. The colonies are flocculent and produce large numbers of aerial hyphae. Day 15: Mycelia grow on the entire dish. Flocky colonies produce aerial hyphae. Mycelia are white. (6) Synthetic Mucor agar medium (25 ° C. culture) Day 10: Medium growth. The colony diameter is 23 mm. Hyphae are white and slightly thin, dendritic, and accompanied by aerial hyphae. 20
Day: Colony diameter is 45 mm. The colony is thin and dendritic.
Mycelia are white. (7) YpSs agar medium (25 ° C. culture) Day 10: Very vigorous growth. The colony diameter is 80 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 15: Mycelia grow on the entire dish. A large amount of dense aerial hyphae are produced. Mycelia are white. (8) Phenol oxidase assay medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 68 mm. Mycelium is white and produces a large amount of aerial mycelia. The medium turned brown. Day 15:
Mycelia grow on the entire dish. A large amount of aerial hyphae,
The entire surface of the medium turns brown. The growth rate significantly varies depending on the old and new seeds. (9) Optimal growth temperature PGY agar medium was inoculated with a discoid inoculum having a diameter of 5 mm, cultured at each temperature for 12 days, and then the colony diameter was measured. As a result, the optimum growth temperature was around 25 ° C. Also, 5
Almost no growth occurred at 35 ° C and 35 ° C. (10) Optimal growth pH PGY liquid medium (PGY agar without agar) 60
Dispense each 100 ml into a 100 ml Erlenmeyer flask to sterilize, adjust each pH with acid or alkali, and inoculate inoculum, then
After static culture at ℃ for 15 days, the dry weight of the cells was measured. As a result, the optimum growth pH was 6-7. Also,
The viable pH range was pH 3.5 to 10.

【００２２】次にリオフィラム ウルマリウム Ｋ−１
２５７株の菌学的諸性質を示す。 （１）麦芽エキス寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は７１mm。白色で放
射状のコロニー、気菌糸を少量生じる。２０日目：シャ
ーレ全体に菌糸が生育する。気菌糸は少ない。菌糸は白
色。 （２）バレイショ・ブドウ糖寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は６６mm。白色で密
な菌糸、気菌糸を多量に生じる。２０日目：シャーレ全
体に菌糸が生育する。表面全体に密な気菌糸を生じる。
コロニーは綿状で白色。 （３）ツァペック・ドックス寒天培地（２５℃培養） ５日目：旺盛な生育。コロニー径は２６mm。樹状に伸長
し極めて希薄な菌糸、気菌糸は少ない。１５日目：コロ
ニー径は７１mm。菌糸は樹状で希薄、白色。２０日目：
コロニー径は８１mm。 （４）サブロー寒天培地（２５℃培養） １０日目：とても旺盛な生育。コロニー径は７９mm。白
色で放射状の密な菌糸、気菌糸やや多い。１５日目：シ
ャーレ全体に菌糸が生育する。気菌糸を生じ、菌糸は放
射状で白色。 （５）オートミール寒天培地（２５℃培養） １０日目：極めて旺盛な生育。コロニー径は７９mm。コ
ロニーは綿状で、多量の気菌糸を生じる。１５日目：シ
ャーレ全体に菌糸が生育する。綿状のコロニーで気菌糸
を生じる。菌糸は白色。 （６）合成ムコール寒天培地（２５℃培養） １０日目：中程度の生育。コロニー径は３７mm。菌糸は
白色でやや薄く、樹枝状に伸長し、気菌糸を伴う。２０
日目：コロニー径は６８mm。コロニーは希薄で樹枝状。
菌糸は白色。 （７）ＹｐＳｓ寒天培地（２５℃培養） １０日目：極めて旺盛な生育。コロニー径は６４mm。白
色で密な菌糸、気菌糸を多量に生じる。１５日目：シャ
ーレ全体に菌糸が生育する。密な気菌糸を多量に生じ
る。菌糸は白色。 （８）フェノールオキシダーゼ検定用培地（２５℃培
養） １０日目：旺盛な生育。コロニー径は７０mm。菌糸は白
色で、気菌糸を多量に生じる。培地は褐変。１５日目：
シャーレ全体に菌糸が生育する。気菌糸を多量に生じ、
培地全面が褐変する。種菌の新旧により著しく生育速度
に差が生じる。 （９）最適生育温度 ＰＧＹ寒天培地に直径５mmの円盤状種菌を接種し、各温
度で１２日間培養した後、コロニー径を測定した。その
結果、最適な成育温度は２５℃付近であった。また、５
℃、３５℃ではほとんど生育しなかった。 (10) 最適生育ｐＨ ＰＧＹ液体培地（寒天を含まないＰＧＹ寒天培地）６０
mlずつを１００ml容三角フラスコに分注して殺菌し、酸
又はアルカリで各ｐＨに調整後に種菌を接種して、２５
℃、１５日間静置培養した後、菌体の乾燥重量を測定し
た。その結果、最適生育ｐＨは６〜８であった。また、
生育可能なｐＨ範囲は、ｐＨ３．５〜１０であった。Next, Riofilum ulmarium K-1
The various bacteriological properties of strain 257 are shown. (1) Malt extract agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 71 mm. White, radial colonies, small amount of aerial mycelia. Day 20: Mycelia grow on the entire dish. There are few aerial hyphae. Mycelia are white. (2) Potato-glucose agar medium (25 ° C. culture) Day 10: Vigorous growth. The colony diameter is 66 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 20: Mycelia grow on the entire dish. Dense aerial hyphae are produced on the entire surface.
The colony is cotton and white. (3) Czapek-Dox agar medium (25 ° C. culture) Day 5: vigorous growth. The colony diameter is 26 mm. Very few mycelia and aerial hyphae that grow in a dendritic form and are extremely thin. Day 15: Colony diameter is 71 mm. Mycelium is dendritic, thin and white. Day 20:
The colony diameter is 81 mm. (4) Sabouraud agar medium (25 ° C. culture) Day 10: Very vigorous growth. The colony diameter is 79 mm. White, radial dense hyphae, aerial hyphae. Day 15: Mycelia grow on the entire dish. Aerial hyphae are produced, and the hyphae are radial and white. (5) Oatmeal agar medium (25 ° C. culture) Day 10: Very vigorous growth. The colony diameter is 79 mm. The colonies are flocculent and produce large numbers of aerial hyphae. Day 15: Mycelia grow on the entire dish. Flocky colonies produce aerial hyphae. Mycelia are white. (6) Synthetic Mucor agar medium (25 ° C. culture) Day 10: Medium growth. The colony diameter is 37 mm. Hyphae are white and slightly thin, dendritic, with aerial hyphae. 20
Day: Colony diameter is 68 mm. The colony is thin and dendritic.
Mycelia are white. (7) YpSs agar medium (25 ° C. culture) Day 10: Very vigorous growth. The colony diameter is 64 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 15: Mycelia grow on the entire dish. A large amount of dense aerial hyphae are produced. Mycelia are white. (8) Phenol oxidase assay medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 70 mm. Mycelium is white and produces a large amount of aerial mycelia. The medium turned brown. Day 15:
Mycelia grow on the entire dish. A large amount of aerial hyphae,
The entire surface of the medium turns brown. The growth rate significantly varies depending on the old and new seeds. (9) Optimal growth temperature PGY agar medium was inoculated with a discoid inoculum having a diameter of 5 mm, cultured at each temperature for 12 days, and then the colony diameter was measured. As a result, the optimum growth temperature was around 25 ° C. Also, 5
Almost no growth occurred at 35 ° C and 35 ° C. (10) Optimal growth pH PGY liquid medium (PGY agar without agar) 60
Dispense each 100 ml into a 100 ml Erlenmeyer flask to sterilize, adjust each pH with acid or alkali, and inoculate inoculum, then
After static culture at ℃ for 15 days, the dry weight of the cells was measured. As a result, the optimum growth pH was 6-8. Also,
The viable pH range was pH 3.5 to 10.

【００２３】次にこのリオフィラム ウルマリウム Ｋ
−６８０４株の菌学的諸性質を示す。 （１）麦芽エキス寒天培地（２５℃培養） ５日目：コロニー径は２５mm。白色で密な菌糸、気菌糸
を多量に生じる。１０日目：コロニー径は６１mm。１５
日目：コロニー径は８３mm。放射状に生育して表面全体
に密な気菌糸を生じる。菌糸は白色。 （２）バレイショ・ブドウ糖寒天培地（２５℃培養） １０日目：中程度の生育。コロニー径は６１mm。白色で
密な菌糸、気菌糸を多量に生じる。１５日目：コロニー
径は８６mm。２０日目：シャーレ全体に菌糸が生育す
る。表面全体に密な気菌糸を生じる。綿状のコロニー
で、菌糸は白色。 （３）ツァペック・ドックス寒天培地（２５℃培養） １０日目：小程度の生育。コロニー径は４９mm。樹状に
伸長し極めて希薄な菌糸、気菌糸は少ない。２０日目：
コロニー径は７６mm。菌糸は樹状で希薄、白色。 （４）サブロー寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は７０mm。白色で綿
状の密な菌糸、気菌糸やや多い。２０日目：シャーレ全
体に菌糸が生育する。放射状に生育して表面全体に気菌
糸を生じる。菌糸は白色。 （５）オートミール寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は７４mm。綿状のコ
ロニーで、気菌糸を生じる。２０日目：シャーレ全体に
菌糸が生育する。綿状のコロニーで、気菌糸を多量に生
じる。菌糸は白色。 （６）合成ムコール寒天培地（２５℃培養） １０日目：小程度の生育。コロニー径は２９mm。菌糸は
白色で、樹枝状のコロニーを形成する。２０日目：コロ
ニー径は５３mm。気菌糸を生じる。樹枝状のコロニー
で、菌糸は白色。 （７）ＹｐＳｓ寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は７２mm。綿状のコ
ロニーで白色な菌糸、気菌糸を生じる。２０日目：シャ
ーレ全体に菌糸が生育する。密な気菌糸を多量に生じ
る。菌糸は白色。 （８）フェノールオキシダーゼ検定用培地（２５℃培
養） ５日目：小程度の生育。コロニー径は２８mm。菌糸は白
色で気菌糸を多量に生じる。培地は褐変。１５日目：シ
ャーレ全体に菌糸が生育する。培地全面が褐変。種菌の
新旧により著しく生育速度に差が生じる。 （９）最適生育温度 ＰＧＹ寒天培地に直径５mmの円盤状種菌を接種し、各温
度で１２日間培養した後、コロニー径を測定した。その
結果、最適な成育温度は２５℃付近であった。また、５
℃、３５℃ではほとんど生育しなかった。 (10) 最適生育ｐＨ ＰＧＹ液体培地（寒天を含まないＰＧＹ寒天培地）６０
mlずつを１００ml容三角フラスコに分注して殺菌し、酸
又はアルカリで各ｐＨに調整後に種菌を接種して、２５
℃、１５日間静置培養した後、菌体の乾燥重量を測定し
た。その結果、最適生育ｐＨは６〜８であった。また、
生育可能なｐＨ範囲は、ｐＨ３．５〜１０であった。Next, this Riofilum ulmarium K
The mycological properties of the -6804 strain are shown. (1) Malt extract agar medium (25 ° C. culture) Day 5: colony diameter 25 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 10: Colony diameter is 61 mm. 15
Day: Colony diameter is 83 mm. It grows radially and produces dense aerial hyphae on the entire surface. Mycelia are white. (2) Potato-glucose agar medium (25 ° C. culture) Day 10: Medium growth. The colony diameter is 61 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 15: colony diameter 86 mm. Day 20: Mycelia grow on the entire dish. Dense aerial hyphae are produced on the entire surface. Cotton-like colony with white hyphae. (3) Czapek-Dox agar medium (25 ° C. culture) Day 10: Small growth. The colony diameter is 49 mm. Very few mycelia and aerial hyphae that grow in a dendritic form and are extremely thin. Day 20:
The colony diameter is 76 mm. Mycelium is dendritic, thin and white. (4) Sabouraud agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 70 mm. White, cotton-like dense hyphae, aerial hyphae. Day 20: Mycelia grow on the entire dish. It grows radially and produces aerial mycelia on the entire surface. Mycelia are white. (5) Oatmeal agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 74 mm. A flocculent colony that produces aerial hyphae. Day 20: Mycelia grow on the entire dish. A flocculent colony with a large amount of aerial hyphae. Mycelia are white. (6) Synthetic Mucor agar medium (25 ° C. culture) Day 10: Small growth. The colony diameter is 29 mm. The hyphae are white and form dendritic colonies. Day 20: Colony diameter 53 mm. Produces aerial mycelia. Dendritic colony with white hyphae. (7) YpSs agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 72 mm. Fluffy colonies produce white hyphae and aerial hyphae. Day 20: Mycelia grow on the entire dish. A large amount of dense aerial hyphae are produced. Mycelia are white. (8) Phenol oxidase assay medium (25 ° C. culture) Day 5: Small growth. The colony diameter is 28 mm. Mycelium is white and produces a large amount of aerial mycelium. The medium turned brown. Day 15: Mycelia grow on the entire dish. The entire surface of the medium turns brown. The growth rate significantly varies depending on the old and new seeds. (9) Optimal growth temperature PGY agar medium was inoculated with a discoid inoculum having a diameter of 5 mm, cultured at each temperature for 12 days, and then the colony diameter was measured. As a result, the optimum growth temperature was around 25 ° C. Also, 5
Almost no growth occurred at 35 ° C and 35 ° C. (10) Optimal growth pH PGY liquid medium (PGY agar without agar) 60
Dispense each 100 ml into a 100 ml Erlenmeyer flask to sterilize, adjust each pH with acid or alkali, and inoculate inoculum, then
After static culture at ℃ for 15 days, the dry weight of the cells was measured. As a result, the optimum growth pH was 6-8. Also,
The viable pH range was pH 3.5 to 10.

【００２４】次にこのリオフィラム ウルマリウム Ｋ
−６８０６株の菌学的諸性質を示す。 （１）麦芽エキス寒天培地（２５℃培養） ５日目：コロニー径は１９mm。白色で密な菌糸、気菌糸
を多量生じる。１０日目：コロニー径は５９mm。１５日
目：コロニー径は８１mm。放射状に生育して表面全体に
密な気菌糸を生じる。菌糸は白色。 （２）バレイショ・ブドウ糖寒天培地（２５℃培養） １０日目：中程度の生育。コロニー径は５５mm。白色で
密な菌糸、気菌糸を多量に生じる。１５日目：コロニー
径は８０mm。２０日目：シャーレ全体に菌糸が生育す
る。表面全体に密な気菌糸を生じる。綿状のコロニー
で、菌糸は白色。 （３）ツァペック・ドックス寒天培地（２５℃培養） １０日目：小程度の生育。コロニー径は３８mm。樹状に
伸長し極めて希薄な菌糸、気菌糸は少ない。２０日目：
コロニー径は６６mm。菌糸は樹状で希薄、白色。 （４）サブロー寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は６６mm。白色で綿
状の密な菌糸、気菌糸やや多い。２０日目：シャーレ全
体に菌糸が生育する。放射状に生育し表面全体に気菌糸
を生じる。菌糸は白色。 （５）オートミール寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は７１mm。綿状のコ
ロニーで、気菌糸を生じる。２０日目：シャーレ全体に
菌糸が生育する。綿状のコロニーで、気菌糸を多量に生
じる。菌糸は白色。 （６）合成ムコール寒天培地（２５℃培養） １０日目：小程度の生育。コロニー径は３１mm。菌糸は
白色で、樹枝状のコロニーを形成する。２０日目：コロ
ニー径は６１mm。気菌糸を生じる。樹枝状のコロニー
で、菌糸は白色。 （７）ＹｐＳｓ寒天培地（２５℃培養） １０日目：旺盛な生育。コロニー径は７０mm。綿状のコ
ロニーで白色な菌糸、気菌糸を生じる。２０日目：シャ
ーレ全体に菌糸が生育する。密な気菌糸を多量に生じ
る。菌糸は白色。 （８）フェノールオキシダーゼ検定用培地（２５℃培
養） ５日目：小程度の生育。コロニー径は２８mm。菌糸は白
色で気菌糸を多量に生じる。培地は褐変。１５日目：旺
盛な生育。コロニー径は８６mm。培地全面が褐変。種菌
の新旧により著しく生育速度に差が生じる。 （９）最適生育温度 ＰＧＹ寒天培地に直径５mmの円盤状種菌を接種し、各温
度で１２日間培養した後、コロニー径を測定した。その
結果、最適な成育温度は２５℃付近であった。また、５
℃、３５℃ではほとんど生育しなかった。 (10) 最適生育ｐＨ ＰＧＹ液体培地（寒天を含まないＰＧＹ寒天培地）６０
mlずつを１００ml容三角フラスコに分注して殺菌し、酸
又はアルカリで各ｐＨに調整後に種菌を接種して、２５
℃、１５日間静置培養した後、菌体の乾燥重量を測定し
た。その結果、最適生育ｐＨは６〜８であった。また、
生育可能なｐＨ範囲は、ｐＨ３．５〜１０であった。Next, this Riofilum ulmarium K
The various bacteriological properties of the −6806 strain are shown. (1) Malt extract agar medium (25 ° C. culture) Day 5: Colony diameter 19 mm. A large amount of white and dense mycelia and aerial mycelia are produced. Day 10: Colony diameter 59 mm. Day 15: colony diameter 81 mm. It grows radially and produces dense aerial hyphae on the entire surface. Mycelia are white. (2) Potato-glucose agar medium (25 ° C. culture) Day 10: Medium growth. The colony diameter is 55 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 15: colony diameter 80 mm. Day 20: Mycelia grow on the entire dish. Dense aerial hyphae are produced on the entire surface. Cotton-like colony with white hyphae. (3) Czapek-Dox agar medium (25 ° C. culture) Day 10: Small growth. The colony diameter is 38 mm. Very few mycelia and aerial hyphae that grow in a dendritic form and are extremely thin. Day 20:
The colony diameter is 66 mm. Mycelium is dendritic, thin and white. (4) Sabouraud agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 66 mm. White, cotton-like dense hyphae, aerial hyphae. Day 20: Mycelia grow on the entire dish. It grows radially and produces aerial hyphae on the entire surface. Mycelia are white. (5) Oatmeal agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 71 mm. A flocculent colony that produces aerial hyphae. Day 20: Mycelia grow on the entire dish. A flocculent colony with a large amount of aerial hyphae. Mycelia are white. (6) Synthetic Mucor agar medium (25 ° C. culture) Day 10: Small growth. The colony diameter is 31 mm. The hyphae are white and form dendritic colonies. Day 20: Colony diameter is 61 mm. Produces aerial mycelia. Dendritic colony with white hyphae. (7) YpSs agar medium (25 ° C. culture) Day 10: vigorous growth. The colony diameter is 70 mm. Fluffy colonies produce white hyphae and aerial hyphae. Day 20: Mycelia grow on the entire dish. A large amount of dense aerial hyphae are produced. Mycelia are white. (8) Phenol oxidase assay medium (25 ° C. culture) Day 5: Small growth. The colony diameter is 28 mm. Mycelium is white and produces a large amount of aerial mycelium. The medium turned brown. Day 15: Strong growth. The colony diameter is 86 mm. The entire surface of the medium turns brown. The growth rate significantly varies depending on the old and new seeds. (9) Optimal growth temperature PGY agar medium was inoculated with a discoid inoculum having a diameter of 5 mm, cultured at each temperature for 12 days, and then the colony diameter was measured. As a result, the optimum growth temperature was around 25 ° C. Also, 5
Almost no growth occurred at 35 ° C and 35 ° C. (10) Optimal growth pH PGY liquid medium (PGY agar without agar) 60
Dispense each 100 ml into a 100 ml Erlenmeyer flask to sterilize, adjust each pH with acid or alkali, and inoculate inoculum, then
After static culture at ℃ for 15 days, the dry weight of the cells was measured. As a result, the optimum growth pH was 6-8. Also,
The viable pH range was pH 3.5 to 10.

【００２５】次に、リオフィラム ウルマリウム Ｋ−
０４２９株、リオフィラム ウルマリウム Ｋ−０２０
７株、リオフィラム ウルマリウム Ｋ−０２０２株、
リオフィラム ウルマリウム Ｋ−０２５９株、リオフ
ィラム ウルマリウム Ｋ−１００４株、リオフィラム
ウルマリウム Ｋ−１２５７株、リオフィラム ウル
マリウム Ｋ−６８０４株及びリオフィラム ウルマリ
ウム Ｋ−６８０６株と他のリオフィラム ウルマリウ
ムとの異同判定として、両菌糸が持つ性因子が異なって
いれば、その菌糸は互いに異なる菌糸であるという菌類
分類学的事実に基づき、性因子の異同を寒天培地上にお
ける対峙培養によって調べた。 供試したリオフィラム ウルマリウムとしてはリオフィ
ラム ウルマリウムＩＦＯ ９６３７、リオフィラム
ウルマリウム ＩＦＯ ３０５２５、リオフィラム ウ
ルマリウム ＩＦＯ ３０７７５、リオフィラム ウル
マリウム Ｌｕ １−２、リオフィラム ウルマリウム
Ｌｕ １−８、リオフィラム ウルマリウム Ｌｕ
１−１７、リオフィラム ウルマリウム Ｍ−８１７
１、リオフィラム ウルマリウム ＳＡ、リオフィラム
ウルマリウム Ｌｕ １−１３である。Next, Riofilum ulmarium K-
0429 strain, riophyllum ulmarium K-020
7 strains, Riofilum ulmarium K-022 strain,
Riofilum ulmarium K-0259 strain, riophyllum ulmarium K-1004 strain, riophyllum ulmarium K-1257 strain, riophyllum ulmarium K-6804 strain and riophyllum ulmarium K-6806 strain and liophyllum ulmarium sexuality possessed by both hyphae. Based on the taxonomic fact that the hyphae are different hyphae if the factors are different from each other, the sex factors were examined by counterculture on an agar medium. The tested Riofilum ulmarium is Riofilum ulmarium IFO 9637, Riofilum
Ulmarium IFO 30525, Riofilum Ulmarium IFO 30775, Riofilum Ulmarium Lu 1-2, Riofilum Ulmarium Lu 1-8, Riofilum Ulmarium Lu
1-17, Riofilum Ulmarium M-817
1. Riofilum ulmarium SA and Riofilum ulmarium Lu 1-13.

【００２６】上記それぞれのリオフィラム ウルマリウ
ムの二核菌糸を保存スラントより３×３×３mmのブロッ
クとして切り出し、それぞれをＰＧＹ寒天培地の中央部
に、リオフィラム ウルマリウム Ｋ−０４２９株、リ
オフィラム ウルマリウムＫ−０２０７株、リオフィラ
ム ウルマリウム Ｋ−０２０２株、リオフィラムウル
マリウム Ｋ−０２５９株、リオフィラム ウルマリウ
ム Ｋ−１００４株、リオフィラム ウルマリウム Ｋ
−１２５７株、リオフィラム ウルマリウム Ｋ−６８
０４株、又はリオフィラム ウルマリウム Ｋ−６８０
６株の二核菌糸と対峙して植菌し（２cm間隔）、２５
℃、１４日間培養後、両菌株のコロニー間に帯線が生じ
るか否かを判定した。結果を表３、表４に示す。（帯線
を生じた場合＋、生じなかった場合−）。Each of the above-mentioned binuclear hyphae of Riofilum ulmarium was cut out from the preserved slant as a block of 3 × 3 × 3 mm, and each of them was placed in the central portion of the PGY agar medium, and Riofilum ulmarium K-0429 strain, Riofilum ulmarium K-0207 strain, Riofilum ulmarium K-0202 strain, riophyllum ulmarium K-0259 strain, riophyllum ulmarium K-1004 strain, riophyllum ulmarium K
-1257 strain, Riofilum ulmarium K-68
04 strain, or Riofilum ulmarium K-680
Inoculate with 6 strains of dinuclear hyphae (2 cm intervals), 25
After culturing at 14 ° C for 14 days, it was determined whether or not a band line was formed between colonies of both strains. The results are shown in Tables 3 and 4. (+ If banding occurred, -if not).

【００２７】[0027]

【表３】 [Table 3]

【００２８】[0028]

【表４】 [Table 4]

【００２９】表３、表４よりわかるようにリオフィラム
ウルマリウム Ｋ−０４２９、リオフィラム ウルマ
リウム Ｋ−０２０７、リオフィラム ウルマリウム
Ｋ−０２０２、リオフィラム ウルマリウム Ｋ−０２
５９、リオフィラム ウルマリウム Ｋ−１００４、リ
オフィラム ウルマリウム Ｋ−１２５７、リオフィラ
ム ウルマリウム Ｋ−６８０４、及びリオフィラム
ウルマリウム Ｋ−６８０６はそれぞれ他の菌株との対
峙培養ですべて帯線を生じ、このことから両菌株は新し
い菌株であることは明白である。As can be seen from Tables 3 and 4, Riofilum ulmarium K-0429, Riophyllum ulmarium K-0207, Riofilum ulmarium
K-0202, Riofilum ulmarium K-02
59, Riofilum ulmarium K-1004, Riofilum ulmarium K-1257, Riofilum ulmarium K-6804, and Riofilum.
Urmalium K-6806 all produced zoning in opposite cultures with other strains, respectively, which makes it clear that both strains are new strains.

【００３０】以上説明したように本発明の交配により育
種した新菌株として、例えばリオフィラム ウルマリウ
ム Ｋ−０４２９、リオフィラム ウルマリウム Ｋ−
０２０７、リオフィラム ウルマリウム Ｋ−０２０
２、リオフィラム ウルマリウム Ｋ−０２５９、リオ
フィラム ウルマリウム Ｋ−１００４、リオフィラム
ウルマリウム Ｋ−１２５７、リオフィラム ウルマリ
ウム Ｋ−６８０４、リオフィラム ウルマリウム Ｋ
−６８０６等が挙げられるが、前記菌株と同様に高苦味
化培地で栽培して得られる子実体の苦味が、少なくとも
一種の高苦味化培地について、苦味をほとんど感じない
程度以下であるという特性を示す菌株はすべて本発明に
属するものである。As the new strains bred by the crossing of the present invention as described above, for example, Riofilum ulmarium K-0429 and Riofilum ulmarium K-.
0207, Riofilum Ulmarium K-020
2, Riofilum ulmarium K-0259, Riofilum ulmarium K-1004, Riofilum ulmarium K-1257, Riofilum ulmarium K-6804, Riofilum ulmarium K
-6806 and the like, but the bitterness of the fruiting body obtained by cultivating in a high bittering medium as in the case of the strain is such that, for at least one type of high bittering medium, the bitterness is less than or equal to a level at which little bitterness is felt. All the strains shown belong to the present invention.

【００３１】本発明による新菌株は、前述のとおり人工
栽培時において高苦味化培地で栽培しても子実体の苦味
が、少なくとも一種の高苦味化培地について、苦味をほ
とんど感じない程度以下であるという特性をもち、培地
の種類に影響されず安定して子実体の苦味が少ない。本
発明の新菌株のＡ、Ｂ、Ｃ、Ｄ培地を用い人工栽培を行
い得た収穫適期の子実体の苦味、及び収量を表５、表６
に示す。なお、前出リオフィラム ウルマリウム ＳＡ
の結果も合せ表６中に示す。As described above, the new strain according to the present invention has a bitterness of fruiting bodies which is less than or equal to the bitterness of at least one type of high bittering medium even when grown in a high bittering medium during artificial cultivation. It has the characteristic that it is stable regardless of the type of medium and has little bitterness in fruiting bodies. Table 5 and Table 6 show the bitterness and yield of fruiting bodies at the proper harvesting time, which were obtained by artificial cultivation using the new strains A, B, C and D of the present invention.
Shown in. The above mentioned Riophyllum ulmarium SA
The results are also shown in Table 6 together.

【００３２】[0032]

【表５】 表 ５ ──────────────────────────────────── リオフィラム ウルマリウム 培 地 収量（ｇ／ビン） 官能検査 ──────────────────────────────────── Ｌｕ １−１３ Ａ １０５ − Ｂ １６８ − Ｃ １４３ − Ｄ １５７ − Ｋ−０４２９ Ａ １１２ − Ｂ １８４ − Ｃ １５７ − Ｄ １６４ − Ｋ−０２０７ Ａ １１１ − Ｂ １８８ − Ｃ １６１ − Ｄ １７８ − Ｋ−０２０２ Ａ ９８ − Ｂ １８７ − Ｃ １５４ − Ｄ １６２ − Ｋ−０２５９ Ａ １０８ − Ｂ １９２ − Ｃ １６２ − Ｄ １６５ − ────────────────────────────────────[Table 5]                                 Table 5 ────────────────────────────────────   Riofilum Ulmarium Cultivated Yield (g / bottle) Sensory test ────────────────────────────────────       Lu 1-13 A 105 −                                   B 168-                                   C143-                                   D157-       K-0429 A 112-                                   B184-                                   C157-                                   D164-       K-0207 A 111-                                   B 188-                                   C 161-                                   D178-       K-022 A 98-                                   B187-                                   C154-                                   D 162-       K-0259 A 108-                                   B 192-                                   C 162 −                                   D165- ────────────────────────────────────

【００３３】[0033]

【表６】 表 ６ ──────────────────────────────────── リオフィラム ウルマリウム 培 地 収量（ｇ／ビン） 官能検査 ──────────────────────────────────── Ｋ−１００４ Ａ ９９ − Ｂ １８８ − Ｃ １５８ − Ｄ １６１ − Ｋ−１２５７ Ａ １０９ − Ｂ １８６ − Ｃ １６０ − Ｄ １５７ − Ｋ−６８０４ Ａ １２０ − Ｂ １９５ − Ｃ １６３ − Ｄ １７１ − Ｋ−６８０６ Ａ １１６ − Ｂ １９１ − Ｃ １６２ − Ｄ １６５ − ＳＡ Ａ １１４ ＋ Ｂ １８９ ＋＋＋ Ｃ １５５ ＋＋ Ｄ １６８ ＋＋＋ ────────────────────────────────────[Table 6]                                 Table 6 ────────────────────────────────────   Riofilum Ulmarium Cultivated Yield (g / bottle) Sensory test ────────────────────────────────────       K-1004 A 99-                                   B 188-                                   C158-                                   D 161-       K-1257 A 109-                                   B186-                                   C 160-                                   D157-       K-6804 A 120-                                   B195-                                   C163-                                   D 171-       K-6806 A 116-                                   B 191-                                   C 162 −                                   D165-       SA A 114 +                                   B 189 +++                                   C 155 ++                                   D 168 +++ ────────────────────────────────────

【００３４】表５、表６に示すようにリオフィラム ウ
ルマリウム Ｌｕ １−１３、リオフィラム ウルマリ
ウム Ｋ−０４２９、リオフィラム ウルマリウム Ｋ
−０２０７、リオフィラム ウルマリウム Ｋ−０２０
２、リオフィラム ウルマリウム Ｋ−０２５９、リオ
フィラム ウルマリウム Ｋ−１００４、リオフィラム
ウルマリウム Ｋ−１２５７、リオフィラム ウルマ
リウム Ｋ−６８０４、リオフィラム ウルマリウム
Ｋ−６８０６の各菌株は、従来の人工栽培可能な菌株に
おいて、収穫適期の子実体が苦味を呈する培地を使用し
ても苦味を示さず、子実体収量を増加させるが苦味も増
加させるので従来使用することが困難であった高苦味化
培地を用いても、苦味の無い子実体を高収率で安定して
得ることができる。As shown in Tables 5 and 6, Riofilum ulmarium Lu 1-13, Riofilum ulmarium K-0429, Riofilum ulmarium K
-0207, Riofilum ulmarium K-020
2, Riophyllum ulmarium K-0259, Riofilum ulmarium K-1004, Riofilum ulmarium K-1257, Riofilum ulmarium K-6804, Riofilum ulmarium
Each of the K-6806 strains does not show bitterness in the conventional artificially cultivated strains even when a medium in which fruiting bodies at the proper harvesting time exhibit bitterness is used, and thus the fruiting body yield is increased but the bitterness is also increased. Even with a high bittering medium that was difficult to use, fruiting bodies without bitterness can be stably obtained in high yield.

【００３５】また、上記菌株を培地に接種して生成する
菌糸体も苦味を呈さず、該菌糸体を食品に使用するにも
好適である。なお、リオフィラム ウルマリウム菌糸体
は食物繊維も多く、制ガン作用も知られており、該菌糸
体を利用した食品は、健康維持のために特に有用であ
る。The mycelium produced by inoculating the above strain into a medium does not show bitterness, and is suitable for use in food. It should be noted that lyophilium ulmarium mycelium has a large amount of dietary fiber and is known to have an anti-cancer effect, and foods using the mycelium are particularly useful for maintaining health.

【００３６】本発明において育種した新菌株のリオフィ
ラム ウルマリウム Ｌｕ １−１３、リオフィラム
ウルマリウム Ｋ−０４２９、リオフィラム ウルマリ
ウムＫ−０２０７、リオフィラム ウルマリウム Ｋ−
０２０２、リオフィラム ウルマリウム Ｋ−０２５
９、リオフィラム ウルマリウム Ｋ−１００４、リオ
フィラム ウルマリウム Ｋ−１２５７、リオフィラム
ウルマリウム Ｋ−６８０４、リオフィラム ウルマ
リウム Ｋ−６８０６はそれぞれ Lyophyllum ulmarium
Ｌｕ １−１３、 Lyophyllum ulmarium Ｋ−０４２
９、 Lyophyllumulmarium Ｋ−０２０７、 Lyophyllum
ulmarium Ｋ−０２０２、 Lyophyllumulmarium Ｋ−
０２５９、 Lyophyllum ulmarium Ｋ−１００４、 Lyo
phyllumulmarium Ｋ−１２５７、 Lyophyllum ulmariu
m Ｋ−６８０４、 Lyophyllumulmarium Ｋ−６８０６
と表示され、工業技術院微生物工業技術研究所にそれぞ
れ微工研菌寄第１２５７１号（ＦＥＲＭＰ−１２５７
１）、微工研菌寄第１２５７０号（ＦＥＲＭ Ｐ−１２
５７０）、微工研菌寄第１２５６９号（ＦＥＲＭＰ−１
２５６９）、微工研菌寄第１２９８０号（ＦＥＲＭ Ｐ
−１２９８０）、微工研菌寄第１２９８１号（ＦＥＲＭ
Ｐ−１２９８１）、微工研菌寄第１２９８２号（ＦＥ
ＲＭ Ｐ−１２９８２）、微工研菌寄第１２９８３号
（ＦＥＲＭＰ−１２９８３）、微工研菌寄第１２９８４
号（ＦＥＲＭ Ｐ−１２９８４）、微工研菌寄第１２９
８５号（ＦＥＲＭ Ｐ−１２９８５）として寄託されて
いる。New strains of the strains bred in the present invention, Riofilum ulmarium Lu 1-13, Riofilum
Ulmarium K-0429, Riofilum Ulmarium K-0207, Riofilum Ulmarium K-
0202, Riofilum ulmarium K-025
9. Lyophyllum ulmarium K-1004, Lyophyllum ulmarium K-1257, Riofilum ulmarium K-6804, and Lyophyllum ulmarium K-6806 are Lyophyllum ulmarium, respectively.
Lu 1-13, Lyophyllum ulmarium K-042
9, Lyophyllumulmarium K-0207, Lyophyllum
ulmarium K-0202, Lyophyllum ulmarium K-
0259, Lyophyllum ulmarium K-1004, Lyo
phyllumulmarium K-1257, Lyophyllum ulmariu
m K-6804, Lyophyllumulmarium K-6806
Is displayed in the Institute of Microbial Technology, National Institute of Advanced Industrial Science and Technology, respectively.
1), Microtechnology Research Institute, No. 12570 (FERM P-12
570), Microtechnology Research Institute, No. 12569 (FERMP-1
2569), Microtech Lab, No. 12980 (FERM P
-12980), and Microtechnology Research Institute, No. 12981 (FERM
P-12981), Microtechnology Research Institute, No. 12982 (FE)
RM P-12982), Microindustry Research Institute No. 12983 (FERMP-12983), Microindustry Research Institute No. 12984.
No. (FERM P-12984), Microcosm Research Institute 129
Deposited as No. 85 (FERM P-12985).

【００３７】なお、リオフィラム ウルマリウム子実体
中の苦味成分は次のように定量することもできる。リオ
フィラム ウルマリウム子実体中の可食部分（カサ部、
柄部）を凍結乾燥し、これを粉砕して子実体凍乾粉末を
得る。この凍乾粉末０．５ｇ（乾重）を３５mlの酢酸エ
チルで２５℃、２４時間振とう抽出し、ろ過、減圧濃縮
後１mlのメタノールに加温しながら溶解し、抽出サンプ
ルとする。前述のサンプル２００μｌより、ＦＰＬＣ
〔ファルマシア ファイン ケミカルス（ Pharmacia F
ine Chemicals ) 製〕を用いて、次の様に苦味成分画分
を分取する。カラムは、Lober ＲＰ−８〔メルク（ Mer
ck )製、φ１．０×２４cm〕を使用して、溶媒８５％メ
タノール（関東化学；液クロ用）、１．０ml／分、常
温、検出波長２１０nmの条件下で、溶出時間１０〜４５
分の画分を分取する。分取画分は再び減圧濃縮し、メタ
ノール：エタノール：水＝３：４：４の溶媒１．０mlに
溶解して、その５０μｌをＨＰＬＣ〔（株）島津製作所
製ＬＣ−６Ａシステム〕にて苦味成分画分を分取する。
カラムは、μ Bondapak Ｃ₁₈〔ウォータース（ Waters
) 製、φ０．３９×３０cm〕を使用して、メタノー
ル：エタノール：水＝３：４：４の溶媒（関東化学；液
クロ用メタノール、ナカライテスク；特級エタノー
ル）、０．７ml／分、温度４０℃、検出波長ＵＶ２１０
nmの条件下で、溶出時間３２．５〜４４．０分の画分の
乾燥重量を測定する。この画分に溶出する成分（以下画
分Ａと略す）は、官能検査において強い苦味を呈し、リ
オフィラム ウルマリウム子実体の苦味は、この画分Ａ
に起因する。The bitterness component in the fruiting body of riophyllum ulmarium can also be quantified as follows. Riofilum ulmarium Edible part in fruiting body (kasa part,
The handle) is freeze-dried, and this is crushed to obtain a fruit-body freeze-dried powder. 0.5 g (dry weight) of this lyophilized powder was extracted with 35 ml of ethyl acetate by shaking at 25 ° C. for 24 hours, filtered, concentrated under reduced pressure, and dissolved in 1 ml of methanol while heating to obtain an extracted sample. FPLC from the above sample 200 μl
[Pharmacia F
ine Chemicals)] and collect the bitter component fraction as follows. Column is Lober RP-8 [Merck (Mer
CK), φ1.0 × 24 cm], solvent 85% methanol (Kanto Chemical; for liquid chromatography), 1.0 ml / min, room temperature, detection wavelength 210 nm, elution time 10-45
Collect fractions of minutes. Min toga fraction was again concentrated under reduced pressure, methanol: ethanol: water = 3: 4: was dissolved in 4 solvent 1.0 ml, the 50μl by HPLC [manufactured by Shimadzu Corporation LC-6A System] The bitterness component fraction is collected.
The column is μ Bondapak C ₁₈ [Waters (Waters
) Manufactured by using φ0.39 × 30cm], methanol: ethanol: water = 3: 4: 4 in a solvent (Kanto Kagaku; liquid chromatography for methanol, Nacalai Tesque; special grade ethanol), 0.7 ml / min, Temperature 40 ℃, detection wavelength UV210
Under the condition of nm, the dry weight of the fraction having an elution time of 32.5 to 44.0 minutes is measured. The component eluting in this fraction (hereinafter abbreviated as Fraction A) exhibits a strong bitterness in the sensory test, and the bitterness of Riofilum ulmarium fruiting body is
caused by.

【００３８】また、本発明で培地に使用した綿実殻は、
安価に入手でき、しかもきのこの収量を著しく増加させ
る、きのこの人工栽培に有用な基材として、本発明者ら
が見出したものである。この綿実殻を培地に使用する際
は、他の培地基材と混合して使用しても良いし、単独で
使用しても良い。綿実殻は培地の乾燥重量に対して１５
〜６０％で使用した場合が最も増収効果が良いが、使用
量はこれに限定されるものではない。この綿実殻を培地
に使用し、収量よく栽培できるきのことしては、例えば
本発明のリオフィラム ウルマリウムの他、シイタケ、
ヒラタケ、エノキタケ、マイタケ等があり、例えばこれ
らのきのこの菌株を使用し、通常の人工栽培を行えば良
い。The cotton seed shells used in the medium of the present invention are
The present invention has been found by the present inventors to be a substrate that can be obtained at low cost and that significantly increases the yield of mushrooms and is useful for artificial cultivation of mushrooms. When this cottonseed shell is used in a medium, it may be mixed with another medium base material or used alone. 15% of cottonseed husks based on dry weight of medium
When used at -60%, the effect of increasing the yield is the best, but the amount used is not limited to this. Mushrooms that can be cultivated in good yield using this cottonseed shell as a medium include, for example, liophyllum ulmarium of the present invention, shiitake mushroom,
There are oyster mushrooms, enoki mushrooms, maitake mushrooms, and the like. For example, strains of these mushrooms may be used for ordinary artificial cultivation.

【００３９】[0039]

【実施例】以下に本発明によるリオフィラム ウルマリ
ウム新菌株の人工栽培実施例を示すが、本発明は以下の
実施例の範囲にのみ限定されるものではない。なお、下
記の実施例１〜実施例３は、本発明の参考例として挙示
した例である。 EXAMPLES Examples of artificial cultivation of a new strain of ryophilum ulmarium according to the present invention are shown below, but the present invention is not limited to the scope of the following examples. In addition, below
Examples 1 to 3 described above are listed as reference examples of the present invention.
It is an example.

【００４０】実施例１ 液体ＰＧＹ培地（寒天を含まないＰＧＹ寒天培地）１０
０mlに、リオフィラムウルマリウム Ｌｕ １−１３
（ＦＥＲＭ Ｐ−１２５７１）を接種し、２５℃で１０
日間培養して液体種菌を得た。一方、コーンコブミール
１３０ｇ、マメカワ６０ｇ、フスマ３０ｇをよく混合し
て水道水により水分含有率を６２％に調製したものを、
ポリプロピレン製８５０ml容広口ビンに圧詰めして、ビ
ン口部中央より下方に向かい直径１cmの穴を開けた後、
該培養基を１２０℃で６０分間高圧蒸気滅菌して、常温
まで冷却後に前記液体種菌２０mlを接種した。暗所、２
５℃、湿度５０〜６０％の条件下で該培養基を３５日間
培養すると、ビン全体に菌糸がまん延した。更に、同条
件下で４０日間培養を続けて子実体発生基を得た。該子
実体発生基の上部菌糸層１cmを除去し、水道水２０mlを
加え充分に給水させた後に余剰の水道水を捨て、１５
℃、湿度９０〜９５％、照度２０ルックスの条件下で９
日間培養を続けて子実体原基を得て、更に照度を２００
ルックスに上げて１３日間培養を続けて子実体を得た。
得られた子実体の収量は１６８ｇで、官能検査において
苦味は感じられなかった。Example 1 Liquid PGY medium (PGY agar containing no agar) 10
To 0 ml, Riofilum ulmarium Lu 1-13
(FERM P-12571) and inoculated at 25 ° C for 10
Liquid culture was obtained by culturing for a day. On the other hand, 130 g of corn cob meal, 60 g of mamekawa and 30 g of bran were mixed well and the water content was adjusted to 62% with tap water.
After compressing it into a polypropylene 850 ml wide-mouthed bottle, punch a hole with a diameter of 1 cm downward from the center of the bottle mouth,
The culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with 20 ml of the liquid inoculum. Dark place, 2
When the culture medium was cultured for 35 days under the conditions of 5 ° C. and a humidity of 50 to 60%, hyphae were spread on the entire bottle. Further, the culture was continued for 40 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating base and adding 20 ml of tap water to supply sufficient water, the excess tap water was discarded.
9 ° C, humidity 90-95%, illuminance 20lux 9
Continue culturing for a day to obtain fruit body primordia, and further illuminance to 200
The seeds were picked up after being cultivated for 15 days and cultured for 13 days.
The yield of the fruiting body thus obtained was 168 g, and no bitterness was felt in the sensory test.

【００４１】なお、子実体の官能検査は、次の様に行っ
た。上記ビン栽培で得られた子実体の柄の基部を除去
し、１本ずつに分け、軽く水洗いして、よく水を切っ
た。一方、フライパンにサラダ油５mlを加え、全体にの
ばし、中火で油を充分に熱した後、子実体１００ｇ（湿
重）を加え、箸でかきまぜながら２分間以上、全体に火
を通し、フライパンに水気が無くなるまで炒め、火を止
めた。油炒めした子実体は常温になるまで冷まし、硫酸
キニーネを標準物質とし、１０名のパネラーで苦味を判
定した。The sensory test of fruiting bodies was conducted as follows. The base of the handle of the fruiting body obtained by the above bottle cultivation was removed, and the fruit body was divided into pieces one by one, lightly washed with water, and well drained. On the other hand, add 5 ml of salad oil to a frying pan, spread it over the whole, heat the oil sufficiently with medium heat, add 100 g (wet weight) of fruiting body, cook for 2 minutes or more with stirring with chopsticks, and cook in the frying pan. Stir until it is dry, then turn off the heat. The fruit body fried in oil was cooled to room temperature, and quinine sulfate was used as a standard substance, and the bitterness was judged by 10 panelists.

【００４２】実施例２ 針葉樹オガクズ５０ｇ、綿実殻粉砕物５０ｇ、米糠１０
０ｇをよく混合して水道水により水分含有率を６３％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後にリオフィラム ウルマリ
ウム Ｌｕ １−１３の固体種菌を接種した。暗所、２
５℃、湿度５０〜６０％の条件下で該培養基を３０日間
培養すると、ビン全体に菌糸がまん延した。更に、同条
件下で３５日間培養を続けて子実体発生基を得た。該子
実体発生基の上部菌糸層１cmを除去し、水道水２０mlを
加え充分に給水させた後に余剰の水道水を捨て、１５
℃、湿度９０〜９５％、照度２０ルックスの条件下で９
日間培養を続けて子実体原基を得て、更に照度を２００
ルックスに上げて１３日間培養を続けて子実体を得た。
得られた子実体の収量は１４３ｇで、子実体の官能検査
において苦味は感じられなかった。Example 2 50 g of coniferous sawdust, 50 g of crushed cottonseed shell, 10 rice bran
0 g was mixed well and the water content was adjusted to 63% with tap water. It was packed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the bottle mouth.
After puncturing, the culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with a solid inoculum of Riofilum ulmarium Lu 1-13. Dark place, 2
When the culture medium was cultured for 30 days under the conditions of 5 ° C. and a humidity of 50 to 60%, hyphae spread to the entire bottle. Further, the culture was continued for 35 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating base and adding 20 ml of tap water to supply sufficient water, the excess tap water was discarded.
9 ° C, humidity 90-95%, illuminance 20lux 9
Continue culturing for a day to obtain fruit body primordia, and further illuminance to 200
The seeds were picked up after being cultivated for 15 days and cultured for 13 days.
The yield of the fruiting body thus obtained was 143 g, and no bitterness was felt in the sensory test of the fruiting body.

【００４３】実施例３ 針葉樹オガクズ１００ｇ、マメカワ７０ｇをよく混合し
て水道水により水分含有率を６３％に調製したものを、
ポリプロピレン製８５０ml容広口ビンに圧詰めして、ビ
ン口部中央より下方に向かい直径１cmの穴を開けた後、
該培養基を１２０℃で６０分間高圧蒸気滅菌して、常温
まで冷却後にリオフィラム ウルマリウム Ｌｕ １−
１３の固体種菌を接種した。暗所、２５℃、湿度５０〜
６０％の条件下で該培養基を３０日間培養すると、ビン
全体に菌糸がまん延した。更に、同条件下で３７日間培
養を続けて子実体発生基を得た。該子実体発生基の上部
菌糸層１cmを除去し、水道水２０mlを加え充分に給水さ
せた後に余剰の水道水を捨て、１５℃、湿度９０〜９５
％、照度２０ルックスの条件下で９日間培養を続けて子
実体原基を得て、更に照度を２００ルックスに上げて１
３日間培養を続けて子実体を得た。得られた子実体の収
量は１５７ｇで、子実体の官能検査において苦味は感じ
られなかった。Example 3 100 g of softwood sawdust and 70 g of Mamekawa were mixed well and the water content was adjusted to 63% with tap water.
After compressing it into a polypropylene 850 ml wide-mouthed bottle, punch a hole with a diameter of 1 cm downward from the center of the bottle mouth,
The culture medium was sterilized by high-pressure steam at 120 ° C for 60 minutes, cooled to room temperature, and then lyophilum ulmarium Lu 1-
Inoculated with 13 solid inoculums. Dark place, 25 ℃, humidity 50 ~
When the culture medium was cultivated for 30 days under the condition of 60%, the mycelium spread over the entire bottle. Further, the culture was continued under the same conditions for 37 days to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water was discarded, and the temperature was 15 ° C. and the humidity was 90 to 95.
%, Culturing was continued for 9 days under the condition of 20 lux to obtain fruit body primordia.
Cultivation was continued for 3 days to obtain fruiting bodies. The yield of the fruiting body obtained was 157 g, and no bitterness was felt in the sensory test of the fruiting body.

【００４４】実施例４ 液体ＰＧＹ培地１００mlに、リオフィラム ウルマリウ
ム Ｋ−０４２９（ＦＥＲＭ Ｐ−１２５７０）を接種
し、２５℃で１０日間培養して液体種菌を得た。一方、
コーンコブミール１３０ｇ、マメカワ６０ｇ、フスマ３
０ｇをよく混合して水道水により水分含有率を６２％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後に前記液体種菌２０mlを接
種した。暗所、２５℃、湿度５０〜６０％の条件下で該
培養基を３２日間培養すると、ビン全体に菌糸がまん延
した。更に、同条件下で４３日間培養を続けて子実体発
生基を得た。該子実体発生基の上部菌糸層１cmを除去
し、水道水２０mlを加え充分に給水させた後に余剰の水
道水を捨て、１５℃、湿度９０〜９５％、照度２０ルッ
クスの条件下で９日間培養を続けて子実体原基を得て、
更に照度を２００ルックスに上げて１３日間培養を続け
て子実体を得た。得られた子実体の収量は１８４ｇで、
子実体の官能検査において苦味は感じられなかった。Example 4 100 ml of liquid PGY medium was inoculated with lyophilum ulmarium K-0429 (FERM P-12570) and cultured at 25 ° C. for 10 days to obtain a liquid inoculum. on the other hand,
Corncob meal 130g, Mamekawa 60g, Bran 3
0 g was mixed well and the water content was adjusted to 62% with tap water. It was pressed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the mouth of the bottle.
After puncturing, the culture medium was sterilized by high pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with 20 ml of the liquid inoculum. When the culture medium was cultured for 32 days in a dark place at 25 ° C. and a humidity of 50 to 60%, hyphae spread to the entire bottle. Furthermore, culture was continued for 43 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water is discarded, and the conditions are 15 ° C., humidity 90 to 95%, and illuminance 20 Lux for 9 days. Continue culturing to obtain fruit body primordia,
The illuminance was further raised to 200 lux and the culture was continued for 13 days to obtain fruiting bodies. The yield of the fruiting body obtained was 184 g,
No bitterness was felt in the sensory test of fruiting bodies.

【００４５】実施例５ 針葉樹オガクズ５０ｇ、綿実殻粉砕物５０ｇ、米糠１０
０ｇをよく混合して水道水により水分含有率を６３％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後にリオフィラム ウルマリ
ウム Ｋ−０４２９の固体種菌を接種した。暗所、２５
℃、湿度５０〜６０％の条件下で該培養基を２８日間培
養すると、ビン全体に菌糸がまん延した。更に、同条件
下で３７日間培養を続けて子実体発生基を得た。該子実
体発生基の上部菌糸層１cmを除去し、水道水２０mlを加
え充分に給水させた後に余剰の水道水を捨て、１５℃、
湿度９０〜９５％、照度２０ルックスの条件下で９日間
培養を続けて子実体原基を得て、更に照度を２００ルッ
クスに上げて１３日間培養を続けて子実体を得た。得ら
れた子実体の収量は１５７ｇで、子実体の官能検査にお
いて苦味は感じられなかった。Example 5 50 g of coniferous sawdust, 50 g of crushed cottonseed shell, 10 rice bran
0 g was mixed well and the water content was adjusted to 63% with tap water. It was packed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the bottle mouth.
, The culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with a solid inoculum of Riofilum ulmarium K-0429. Dark place, 25
When the culture medium was cultured for 28 days under the condition of the temperature of 50 ° C. and the humidity of 50 to 60%, the mycelia were spread over the entire bottle. Further, the culture was continued under the same conditions for 37 days to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating base and adding 20 ml of tap water to supply water sufficiently, the surplus tap water was discarded and the temperature was raised to 15 ° C.
Cultivation was continued for 9 days under conditions of humidity of 90 to 95% and illuminance of 20 lux to obtain fruit body primordia, and the illuminance was further raised to 200 lux for 13 days of culturing to obtain fruit bodies. The yield of the fruiting body obtained was 157 g, and no bitterness was felt in the sensory test of the fruiting body.

【００４６】実施例６ 針葉樹オガクズ１００ｇ、マメカワ７０ｇをよく混合し
て水道水により水分含有率を６３％に調製したものを、
ポリプロピレン製８５０ml容広口ビンに圧詰めして、ビ
ン口部中央より下方に向かい直径１cmの穴を開けた後、
該培養基を１２０℃で６０分間高圧蒸気滅菌して、常温
まで冷却後にリオフィラム ウルマリウム Ｋ−０４２
９の固体種菌を接種した。暗所、２５℃、湿度５０〜６
０％の条件下で該培養基を２８日間培養すると、ビン全
体に菌糸がまん延した。更に、同条件下で３７日間培養
を続けて子実体発生基を得た。該子実体発生基の上部菌
糸層１cmを除去し、水道水２０mlを加え充分に給水させ
た後に余剰の水道水を捨て、１５℃、湿度９０〜９５
％、照度２０ルックスの条件下で９日間培養を続けて子
実体原基を得て、更に照度を２００ルックスに上げて１
３日間培養を続けて子実体を得た。得られた子実体の収
量は１６４ｇで、子実体の官能検査において苦味は感じ
られなかった。Example 6 100 g of coniferous sawdust and 70 g of Mamekawa were mixed well and the water content was adjusted to 63% with tap water.
After compressing it into a polypropylene 850 ml wide-mouthed bottle, punch a hole with a diameter of 1 cm downward from the center of the bottle mouth,
The culture medium was sterilized by high-pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and then Lyophilum ulmarium K-042.
9 solid inoculums were inoculated. Dark place, 25 ° C, humidity 50-6
When the culture medium was cultivated for 28 days under the condition of 0%, the mycelium spread over the entire bottle. Further, the culture was continued under the same conditions for 37 days to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water was discarded, and the temperature was 15 ° C. and the humidity was 90 to 95.
%, Culturing was continued for 9 days under the condition of 20 lux to obtain fruit body primordia.
Cultivation was continued for 3 days to obtain fruiting bodies. The yield of the fruiting body thus obtained was 164 g, and no bitterness was felt in the sensory test of the fruiting body.

【００４７】実施例７ 液体ＰＧＹ培地１００mlに、リオフィラム ウルマリウ
ム Ｋ−０２０７（ＦＥＲＭ Ｐ−１２５６９）を接種
し、２５℃で１０日間培養して液体種菌を得た。一方、
コーンコブミール１３０ｇ、マメカワ６０ｇ、フスマ３
０ｇをよく混合して水道水により水分含有率を６２％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後に前記液体種菌２０mlを接
種した。暗所、２５℃、湿度５０〜６０％の条件下で該
培養基を４０日間培養すると、ビン全体に菌糸がまん延
した。更に、同条件下で３５日間培養を続けて子実体発
生基を得た。該子実体発生基の上部菌糸層１cmを除去
し、水道水２０mlを加え充分に給水させた後に余剰の水
道水を捨て、１５℃、湿度９０〜９５％、照度２０ルッ
クスの条件下で１０日間培養を続けて子実体原基を得
て、更に照度を２００ルックスに上げて１４日間培養を
続けて子実体を得た。得られた子実体の収量は１８８ｇ
で、子実体の官能検査において苦味は感じられなかっ
た。Example 7 100 ml of a liquid PGY medium was inoculated with lyophilum ulmarium K-0207 (FERM P-12569) and cultured at 25 ° C. for 10 days to obtain a liquid inoculum. on the other hand,
Corncob meal 130g, Mamekawa 60g, Bran 3
0 g was mixed well and the water content was adjusted to 62% with tap water. It was pressed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the mouth of the bottle.
After puncturing, the culture medium was sterilized by high pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with 20 ml of the liquid inoculum. When the culture medium was cultured for 40 days in the dark at 25 ° C. and a humidity of 50 to 60%, hyphae were spread over the entire bottle. Further, the culture was continued for 35 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water is discarded, and the conditions are 15 ° C, humidity 90 to 95%, and illuminance 20 lux for 10 days. The culturing was continued to obtain a fruiting body primordium, the illuminance was further raised to 200 lux, and the cultivation was continued for 14 days to obtain a fruiting body. The yield of fruiting body obtained is 188 g.
No bitterness was felt in the sensory test of fruiting bodies.

【００４８】実施例８ 針葉樹オガクズ５０ｇ、綿実殻粉砕物５０ｇ、米糠１０
０ｇをよく混合して水道水により水分含有率を６３％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後にリオフィラム ウルマリ
ウム Ｋ−０２０７の固体種菌を接種した。暗所、２５
℃、湿度５０〜６０％の条件下で該培養基を３４日間培
養すると、ビン全体に菌糸がまん延した。更に、同条件
下で３１日間培養を続けて子実体発生基を得た。該子実
体発生基の上部菌糸層１cmを除去し、水道水２０mlを加
え充分に給水させた後に余剰の水道水を捨て、１５℃、
湿度９０〜９５％、照度２０ルックスの条件下で９日間
培養を続けて子実体原基を得て、更に照度を２００ルッ
クスに上げて１４日間培養を続けて子実体を得た。得ら
れた子実体の収量は１６１ｇで、子実体の官能検査にお
いて苦味は感じられなかった。Example 8 50 g of coniferous sawdust, 50 g of crushed cottonseed shell, 10 rice bran
0 g was mixed well and the water content was adjusted to 63% with tap water. It was packed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the bottle mouth.
, The culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with a solid inoculum of Riofilum ulmarium K-0207. Dark place, 25
When the culture medium was cultivated for 34 days at a temperature of 50 ° C. and a humidity of 50 to 60%, hyphae spread over the entire bottle. Further, the culture was continued for 31 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating base and adding 20 ml of tap water to supply water sufficiently, the surplus tap water was discarded and the temperature was raised to 15 ° C.
Cultivation was continued for 9 days under conditions of humidity of 90 to 95% and illuminance of 20 lux to obtain a fruiting body primordium. Further, the illuminance was increased to 200 lux and culturing was continued for 14 days to obtain fruiting bodies. The yield of the fruiting body thus obtained was 161 g, and no bitterness was felt in the sensory test of the fruiting body.

【００４９】実施例９ 針葉樹オガクズ１００ｇ、マメカワ７０ｇをよく混合し
て水道水により水分含有率を６３％に調製したものを、
ポリプロピレン製８５０ml容広口ビンに圧詰めして、ビ
ン口部中央より下方に向かい直径１cmの穴を開けた後、
該培養基を１２０℃で６０分間高圧蒸気滅菌して、常温
まで冷却後にリオフィラム ウルマリウム Ｋ−０２０
７の固体種菌を接種した。暗所、２５℃、湿度５０〜６
０％の条件下で該培養基を３３日間培養すると、ビン全
体に菌糸がまん延した。更に、同条件下で３２日間培養
を続けて子実体発生基を得た。該子実体発生基の上部菌
糸層１cmを除去し、水道水２０mlを加え充分に給水させ
た後に余剰の水道水を捨て、１５℃、湿度９０〜９５
％、照度２０ルックスの条件下で１１日間培養を続けて
子実体原基を得て、更に照度を２００ルックスに上げて
１３日間培養を続けて子実体を得た。得られた子実体の
収量は１７８ｇで、子実体の官能検査において苦味は感
じられなかった。Example 9 100 g of coniferous sawdust and 70 g of Mamekawa were mixed well and the water content was adjusted to 63% with tap water.
After compressing it into a polypropylene 850 ml wide-mouthed bottle, punch a hole with a diameter of 1 cm downward from the center of the bottle mouth,
The culture medium was sterilized by high-pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and then Lyophilum ulmarium K-020.
7 solid inoculums were inoculated. Dark place, 25 ° C, humidity 50-6
When the culture medium was cultured for 33 days under the condition of 0%, the mycelium spread over the entire bottle. Further, the culture was continued for 32 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water was discarded, and the temperature was 15 ° C. and the humidity was 90 to 95.
%, The culturing was continued for 11 days under the condition of 20 lux to obtain fruit body primordia, and the illuminance was further raised to 200 lux for 13 days to continue cultivation to obtain fruit bodies. The yield of the fruiting body thus obtained was 178 g, and no bitterness was felt in the sensory test of the fruiting body.

【００５０】実施例１０ 液体ＰＧＹ培地１００mlに、リオフィラム ウルマリウ
ム Ｋ−０２０２（ＦＥＲＭ Ｐ−１２９８０）を接種
し、２５℃で１０日間培養して液体種菌を得た。一方、
コーンコブミール１３０ｇ、マメカワ６０ｇ、フスマ３
０ｇをよく混合して水道水により水分含有率を６２％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後に前記液体種菌２０mlを接
種した。暗所、２５℃、湿度５０〜６０％の条件下で該
培養基を４５日間培養すると、ビン全体に菌糸がまん延
した。更に、同条件下で３５日間培養を続けて子実体発
生基を得た。該子実体発生基の上部菌糸層１cmを除去
し、水道水２０mlを加え充分に給水させた後に余剰の水
道水を捨て、１５℃、湿度９０〜９５％、照度２０ルッ
クスの条件下で８日間培養を続けて子実体原基を得て、
更に照度を２００ルックスに上げて１３日間培養を続け
て子実体を得た。得られた子実体の収量は１８７ｇで、
子実体の官能検査において苦味は感じられなかった。Example 10 100 ml of a liquid PGY medium was inoculated with riophyllum ulmarium K-0202 (FERM P-12980) and cultured at 25 ° C. for 10 days to obtain a liquid inoculum. on the other hand,
Corncob meal 130g, Mamekawa 60g, Bran 3
0 g was mixed well and the water content was adjusted to 62% with tap water. It was pressed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the mouth of the bottle.
After puncturing, the culture medium was sterilized by high pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with 20 ml of the liquid inoculum. When the culture medium was cultured for 45 days in a dark place at 25 ° C. and a humidity of 50 to 60%, mycelia were spread over the entire bottle. Further, the culture was continued for 35 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to supply water sufficiently, the excess tap water was discarded, and the conditions were 15 ° C., humidity 90 to 95%, and illuminance 20 Lux for 8 days. Continue culturing to obtain fruit body primordia,
The illuminance was further raised to 200 lux and the culture was continued for 13 days to obtain fruiting bodies. The yield of the fruiting body obtained was 187 g,
No bitterness was felt in the sensory test of fruiting bodies.

【００５１】実施例１１ 針葉樹オガクズ５０ｇ、綿実殻粉砕物５０ｇ、米糠１０
０ｇをよく混合して水道水により水分含有率を６３％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後にリオフィラム ウルマリ
ウム Ｋ−０２０２の固体種菌を接種した。暗所、２５
℃、湿度５０〜６０％の条件下で該培養基を４０日間培
養すると、ビン全体に菌糸がまん延した。更に、同条件
下で４０日間培養を続けて子実体発生基を得た。該子実
体発生基の上部菌糸層１cmを除去し、水道水２０mlを加
え充分に給水させた後に余剰の水道水を捨て、１５℃、
湿度９０〜９５％、照度２０ルックスの条件下で８日間
培養を続けて子実体原基を得て、更に照度を２００ルッ
クスに上げて１２日間培養を続けて子実体を得た。得ら
れた子実体の収量は１５４ｇで、子実体の官能検査にお
いて苦味は感じられなかった。Example 11 50 g of coniferous sawdust, 50 g of crushed cottonseed shell, 10 rice bran
0 g was mixed well and the water content was adjusted to 63% with tap water. It was packed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the bottle mouth.
After puncturing, the culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with a solid inoculum of Riofilum ulmarium K-0202. Dark place, 25
When the culture medium was cultivated for 40 days at a temperature of 50 ° C. and a humidity of 50% to 60%, hyphae were spread over the entire bottle. Further, the culture was continued for 40 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating base and adding 20 ml of tap water to supply water sufficiently, the surplus tap water was discarded and the temperature was raised to 15 ° C.
Cultivation was continued under the conditions of humidity of 90 to 95% and illumination of 20 lux for 8 days to obtain fruit body primordium, and the illuminance was further raised to 200 lux to continue culture for 12 days to obtain fruit bodies. The yield of the fruiting body thus obtained was 154 g, and no bitterness was felt in the sensory test of the fruiting body.

【００５２】実施例１２ 針葉樹オガクズ１００ｇ、マメカワ７０ｇをよく混合し
て水道水により水分含有率を６３％に調製したものを、
ポリプロピレン製８５０ml容広口ビンに圧詰めして、ビ
ン口部中央より下方に向かい直径１cmの穴を開けた後、
該培養基を１２０℃で６０分間高圧蒸気滅菌して、常温
まで冷却後にリオフィラム ウルマリウム Ｋ−０２０
２の固体種菌を接種した。暗所、２５℃、湿度５０〜６
０％の条件下で該培養基を３６日間培養すると、ビン全
体に菌糸がまん延した。更に、同条件下で４４日間培養
を続けて子実体発生基を得た。該子実体発生基の上部菌
糸層１cmを除去し、水道水２０mlを加え充分に給水させ
た後に余剰の水道水を捨て、１５℃、湿度９０〜９５
％、照度２０ルックスの条件下で８日間培養を続けて子
実体原基を得て、更に照度を２００ルックスに上げて１
３日間培養を続けて子実体を得た。得られた子実体の収
量は１６２ｇで、子実体の官能検査において苦味は感じ
られなかった。Example 12 100 g of coniferous sawdust and 70 g of Mamekawa were mixed well and the water content was adjusted to 63% with tap water.
After compressing it into a polypropylene 850 ml wide-mouthed bottle, punch a hole with a diameter of 1 cm downward from the center of the bottle mouth,
The culture medium was sterilized by high-pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and then Lyophilum ulmarium K-020.
Two solid inoculums were inoculated. Dark place, 25 ° C, humidity 50-6
When the culture medium was cultivated for 36 days under the condition of 0%, the mycelium spread over the entire bottle. Furthermore, the culture was continued for 44 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water was discarded, and the temperature was 15 ° C. and the humidity was 90 to 95.
%, The illuminance was 20 lux, and the culture was continued for 8 days to obtain fruit body primordia.
Cultivation was continued for 3 days to obtain fruiting bodies. The yield of the fruiting body obtained was 162 g, and no bitterness was felt in the sensory test of the fruiting body.

【００５３】実施例１３ 液体ＰＧＹ培地１００mlに、リオフィラム ウルマリウ
ム Ｋ−０２５９（ＦＥＲＭ Ｐ−１２９８１）を接種
し、２５℃で１０日間培養して液体種菌を得た。一方、
コーンコブミール１３０ｇ、マメカワ６０ｇ、フスマ３
０ｇをよく混合して水道水により水分含有率を６２％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後に前記液体種菌２０mlを接
種した。暗所、２５℃、湿度５０〜６０％の条件下で該
培養基を４５日間培養すると、ビン全体に菌糸がまん延
した。更に、同条件下で３５日間培養を続けて子実体発
生基を得た。該子実体発生基の上部菌糸層１cmを除去
し、水道水２０mlを加え充分に給水させた後に余剰の水
道水を捨て、１５℃、湿度９０〜９５％、照度２０ルッ
クスの条件下で８日間培養を続けて子実体原基を得て、
更に照度を２００ルックスに上げて１２日間培養を続け
て子実体を得た。得られた子実体の収量は１９２ｇで、
子実体の官能検査において苦味は感じられなかった。Example 13 100 ml of the liquid PGY medium was inoculated with lyophilum ulmarium K-0259 (FERM P-12981) and cultured at 25 ° C. for 10 days to obtain a liquid inoculum. on the other hand,
Corncob meal 130g, Mamekawa 60g, Bran 3
0 g was mixed well and the water content was adjusted to 62% with tap water. It was pressed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the mouth of the bottle.
After puncturing, the culture medium was sterilized by high pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with 20 ml of the liquid inoculum. When the culture medium was cultured for 45 days in a dark place at 25 ° C. and a humidity of 50 to 60%, mycelia were spread over the entire bottle. Further, the culture was continued for 35 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to supply water sufficiently, the excess tap water was discarded, and the conditions were 15 ° C., humidity 90 to 95%, and illuminance 20 Lux for 8 days. Continue culturing to obtain fruit body primordia,
The illuminance was further raised to 200 lux and the culture was continued for 12 days to obtain fruiting bodies. The yield of the fruiting body obtained was 192 g,
No bitterness was felt in the sensory test of fruiting bodies.

【００５４】実施例１４ 針葉樹オガクズ５０ｇ、綿実殻粉砕物５０ｇ、米糠１０
０ｇをよく混合して水道水により水分含有率を６３％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後にリオフィラム ウルマリ
ウム Ｋ−０２５９の固体種菌を接種した。暗所、２５
℃、湿度５０〜６０％の条件下で該培養基を３５日間培
養すると、ビン全体に菌糸がまん延した。更に、同条件
下で４５日間培養を続けて子実体発生基を得た。該子実
体発生基の上部菌糸層１cmを除去し、水道水２０mlを加
え充分に給水させた後に余剰の水道水を捨て、１５℃、
湿度９０〜９５％、照度２０ルックスの条件下で８日間
培養を続けて子実体原基を得て、更に照度を２００ルッ
クスに上げて１３日間培養を続けて子実体を得た。得ら
れた子実体の収量は１６２ｇで、子実体の官能検査にお
いて苦味は感じられなかった。Example 14 50 g of coniferous sawdust, 50 g of crushed cottonseed shell, 10 rice bran
0 g was mixed well and the water content was adjusted to 63% with tap water. It was packed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the bottle mouth.
After puncturing, the culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with a solid inoculum of Riofilum ulmarium K-0259. Dark place, 25
When the culture medium was cultivated for 35 days at 50 ° C. and a humidity of 50 to 60%, mycelia spread to the entire bottle. Further, the culture was continued for 45 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating base and adding 20 ml of tap water to supply water sufficiently, the surplus tap water was discarded and the temperature was raised to 15 ° C.
Cultivation was continued for 8 days under conditions of humidity of 90 to 95% and illuminance of 20 lux to obtain fruit body primordium. Further, the illuminance was increased to 200 lux and culturing was continued for 13 days to obtain fruit body. The yield of the fruiting body obtained was 162 g, and no bitterness was felt in the sensory test of the fruiting body.

【００５５】実施例１５ 針葉樹オガクズ１００ｇ、マメカワ７０ｇをよく混合し
て水道水により水分含有率を６３％に調製したものを、
ポリプロピレン製８５０ml容広口ビンに圧詰めして、ビ
ン口部中央より下方に向かい直径１cmの穴を開けた後、
該培養基を１２０℃で６０分間高圧蒸気滅菌して、常温
まで冷却後にリオフィラム ウルマリウム Ｋ−０２５
９の固体種菌を接種した。暗所、２５℃、湿度５０〜６
０％の条件下で該培養基を３７日間培養すると、ビン全
体に菌糸がまん延した。更に、同条件下で４３日間培養
を続けて子実体発生基を得た。該子実体発生基の上部菌
糸層１cmを除去し、水道水２０mlを加え充分に給水させ
た後に余剰の水道水を捨て、１５℃、湿度９０〜９５
％、照度２０ルックスの条件下で８日間培養を続けて子
実体原基を得て、更に照度を２００ルックスに上げて１
３日間培養を続けて子実体を得た。得られた子実体の収
量は１６５ｇで、子実体の官能検査において苦味は感じ
られなかった。Example 15 100 g of coniferous sawdust and 70 g of Mamekawa were mixed well and the water content was adjusted to 63% with tap water.
After compressing it into a polypropylene 850 ml wide-mouthed bottle, punch a hole with a diameter of 1 cm downward from the center of the bottle mouth,
The culture medium was sterilized by high-pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and then Lyophilum ulmarium K-025.
9 solid inoculums were inoculated. Dark place, 25 ° C, humidity 50-6
When the culture medium was cultivated for 37 days under the condition of 0%, the mycelium spread over the entire bottle. Furthermore, culture was continued for 43 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water was discarded, and the temperature was 15 ° C. and the humidity was 90 to 95.
%, The illuminance was 20 lux, and the culture was continued for 8 days to obtain fruit body primordia.
Cultivation was continued for 3 days to obtain fruiting bodies. The yield of the fruiting body thus obtained was 165 g, and no bitterness was felt in the sensory test of the fruiting body.

【００５６】実施例１６ 液体ＰＧＹ培地１００mlに、リオフィラム ウルマリウ
ム Ｋ−１００４（ＦＥＲＭ Ｐ−１２９８２）を接種
し、２５℃で１０日間培養して液体種菌を得た。一方、
コーンコブミール１３０ｇ、マメカワ６０ｇ、フスマ３
０ｇをよく混合して水道水により水分含有率を６２％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後に前記液体種菌２０mlを接
種した。暗所、２５℃、湿度５０〜６０％の条件下で該
培養基を３０日間培養すると、ビン全体に菌糸がまん延
した。更に、同条件下で５０日間培養を続けて子実体発
生基を得た。該子実体発生基の上部菌糸層１cmを除去
し、水道水２０mlを加え充分に給水させた後に余剰の水
道水を捨て、１５℃、湿度９０〜９５％、照度２０ルッ
クスの条件下で１１日間培養を続けて子実体原基を得
て、更に照度を２００ルックスに上げて１５日間培養を
続けて子実体を得た。得られた子実体の収量は１８８ｇ
で、子実体の官能検査において苦味は感じられなかっ
た。Example 16 100 ml of liquid PGY medium was inoculated with riophyllum ulmarium K-1004 (FERM P-12982) and cultured at 25 ° C. for 10 days to obtain a liquid inoculum. on the other hand,
Corncob meal 130g, Mamekawa 60g, Bran 3
0 g was mixed well and the water content was adjusted to 62% with tap water. It was pressed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the mouth of the bottle.
After puncturing, the culture medium was sterilized by high pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with 20 ml of the liquid inoculum. When the culture medium was cultured for 30 days in the dark at 25 ° C. and a humidity of 50 to 60%, the mycelium spread to the entire bottle. Furthermore, the culture was continued for 50 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group, adding 20 ml of tap water and supplying water sufficiently, the excess tap water was discarded, and the conditions were 15 ° C., humidity 90 to 95%, and illuminance 20 lux for 11 days. The culturing was continued to obtain a fruiting body primordium, the illuminance was further raised to 200 lux, and the cultivation was continued for 15 days to obtain a fruiting body. The yield of fruiting body obtained is 188 g.
No bitterness was felt in the sensory test of fruiting bodies.

【００５７】実施例１７ 針葉樹オガクズ５０ｇ、綿実殻粉砕物５０ｇ、米糠１０
０ｇをよく混合して水道水により水分含有率を６３％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後にリオフィラム ウルマリ
ウム Ｋ−１００４の固体種菌を接種した。暗所、２５
℃、湿度５０〜６０％の条件下で該培養基を３２日間培
養すると、ビン全体に菌糸がまん延した。更に、同条件
下で４８日間培養を続けて子実体発生基を得た。該子実
体発生基の上部菌糸層１cmを除去し、水道水２０mlを加
え充分に給水させた後に余剰の水道水を捨て、１５℃、
湿度９０〜９５％、照度２０ルックスの条件下で１０日
間培養を続けて子実体原基を得て、更に照度を２００ル
ックスに上げて１５日間培養を続けて子実体を得た。得
られた子実体の収量は１５８ｇで、子実体の官能検査に
おいて苦味は感じられなかった。Example 17 50 g of coniferous sawdust, 50 g of crushed cottonseed shell, 10 rice bran
0 g was mixed well and the water content was adjusted to 63% with tap water. It was packed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the bottle mouth.
After puncturing, the culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with a solid inoculum of Riofilum ulmarium K-1004. Dark place, 25
When the culture medium was cultivated for 32 days at a temperature of 50 ° C. and a humidity of 50 to 60%, hyphae were spread over the entire bottle. Further, the culture was continued for 48 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating base and adding 20 ml of tap water to supply water sufficiently, the surplus tap water was discarded and the temperature was raised to 15 ° C.
Cultivation was continued for 10 days under conditions of humidity of 90 to 95% and illuminance of 20 lux to obtain fruit body primordium. Further, the illuminance was increased to 200 lux and culturing was continued for 15 days to obtain fruit body. The yield of the fruiting body thus obtained was 158 g, and no bitterness was felt in the sensory test of the fruiting body.

【００５８】実施例１８ 針葉樹オガクズ１００ｇ、マメカワ７０ｇをよく混合し
て水道水により水分含有率を６３％に調製したものを、
ポリプロピレン製８５０ml容広口ビンに圧詰めして、ビ
ン口部中央より下方に向かい直径１cmの穴を開けた後、
該培養基を１２０℃で６０分間高圧蒸気滅菌して、常温
まで冷却後にリオフィラム ウルマリウム Ｋ−１００
４の固体種菌を接種した。暗所、２５℃、湿度５０〜６
０％の条件下で該培養基を２８日間培養すると、ビン全
体に菌糸がまん延した。更に、同条件下で５２日間培養
を続けて子実体発生基を得た。該子実体発生基の上部菌
糸層１cmを除去し、水道水２０mlを加え充分に給水させ
た後に余剰の水道水を捨て、１５℃、湿度９０〜９５
％、照度２０ルックスの条件下で１１日間培養を続けて
子実体原基を得て、更に照度を２００ルックスに上げて
１６日間培養を続けて子実体を得た。得られた子実体の
収量は１６１ｇで、子実体の官能検査において苦味は感
じられなかった。Example 18 100 g of coniferous sawdust and 70 g of Mamekawa were mixed well and the water content was adjusted to 63% with tap water.
After compressing it into a polypropylene 850 ml wide-mouthed bottle, punch a hole with a diameter of 1 cm downward from the center of the bottle mouth,
The culture medium was sterilized by high pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and then lyophilum ulmarium K-100.
4 solid inoculums were inoculated. Dark place, 25 ° C, humidity 50-6
When the culture medium was cultivated for 28 days under the condition of 0%, the mycelium spread over the entire bottle. Further, the culture was continued for 52 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water was discarded, and the temperature was 15 ° C. and the humidity was 90 to 95.
%, And the illuminance was 20 lux, the culture was continued for 11 days to obtain a fruit body primordium, and the illuminance was further raised to 200 lux to continue the culture for 16 days to obtain a fruit body. The yield of the fruiting body thus obtained was 161 g, and no bitterness was felt in the sensory test of the fruiting body.

【００５９】実施例１９ 液体ＰＧＹ培地１００mlに、リオフィラム ウルマリウ
ム Ｋ−１２５７（ＦＥＲＭ Ｐ−１２９８３）を接種
し、２５℃で１０日間培養して液体種菌を得た。一方、
コーンコブミール１３０ｇ、マメカワ６０ｇ、フスマ３
０ｇをよく混合して水道水により水分含有率を６２％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後に前記液体種菌２０mlを接
種した。暗所、２５℃、湿度５０〜６０％の条件下で該
培養基を３０日間培養すると、ビン全体に菌糸がまん延
した。更に、同条件下で５０日間培養を続けて子実体発
生基を得た。該子実体発生基の上部菌糸層１cmを除去
し、水道水２０mlを加え充分に給水させた後に余剰の水
道水を捨て、１５℃、湿度９０〜９５％、照度２０ルッ
クスの条件下で１１日間培養を続けて子実体原基を得
て、更に照度を２００ルックスに上げて１５日間培養を
続けて子実体を得た。得られた子実体の収量は１８６ｇ
で、子実体の官能検査において苦味は感じられなかっ
た。Example 19 100 ml of liquid PGY medium was inoculated with riophyllum ulmarium K-1257 (FERM P-12983) and cultured at 25 ° C. for 10 days to obtain a liquid inoculum. on the other hand,
Corncob meal 130g, Mamekawa 60g, Bran 3
0 g was mixed well and the water content was adjusted to 62% with tap water. It was pressed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the mouth of the bottle.
After puncturing, the culture medium was sterilized by high pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with 20 ml of the liquid inoculum. When the culture medium was cultured for 30 days in the dark at 25 ° C. and a humidity of 50 to 60%, the mycelium spread to the entire bottle. Furthermore, the culture was continued for 50 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group, adding 20 ml of tap water and supplying water sufficiently, the excess tap water was discarded, and the conditions were 15 ° C., humidity 90 to 95%, and illuminance 20 lux for 11 days. The culturing was continued to obtain a fruiting body primordium, the illuminance was further raised to 200 lux, and the cultivation was continued for 15 days to obtain a fruiting body. The yield of the fruiting body obtained is 186 g.
No bitterness was felt in the sensory test of fruiting bodies.

【００６０】実施例２０ 針葉樹オガクズ５０ｇ、綿実殻粉砕物５０ｇ、米糠１０
０ｇをよく混合して水道水により水分含有率を６３％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後にリオフィラム ウルマリ
ウム Ｋ−１２５７の固体種菌を接種した。暗所、２５
℃、湿度５０〜６０％の条件下で該培養基を３２日間培
養すると、ビン全体に菌糸がまん延した。更に、同条件
下で４８日間培養を続けて子実体発生基を得た。該子実
体発生基の上部菌糸層１cmを除去し、水道水２０mlを加
え充分に給水させた後に余剰の水道水を捨て、１５℃、
湿度９０〜９５％、照度２０ルックスの条件下で９日間
培養を続けて子実体原基を得て、更に照度を２００ルッ
クスに上げて１６日間培養を続けて子実体を得た。得ら
れた子実体の収量は１６０ｇで、子実体の官能検査にお
いて苦味は感じられなかった。Example 20 50 g of coniferous sawdust, 50 g of crushed cottonseed shell, 10 rice bran
0 g was mixed well and the water content was adjusted to 63% with tap water. It was packed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the bottle mouth.
After puncturing, the culture medium was sterilized by high-pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with a solid inoculum of Riophyllum ulmarium K-1257. Dark place, 25
When the culture medium was cultivated for 32 days at a temperature of 50 ° C. and a humidity of 50 to 60%, hyphae were spread over the entire bottle. Further, the culture was continued for 48 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating base and adding 20 ml of tap water to supply water sufficiently, the surplus tap water was discarded and the temperature was raised to 15 ° C.
Cultivation was continued under the conditions of humidity of 90 to 95% and illumination of 20 lux for 9 days to obtain a fruiting body primordium, and the illuminance was further raised to 200 lux to continue culturing for 16 days to obtain a fruiting body. The yield of the fruiting body thus obtained was 160 g, and no bitterness was felt in the sensory test of the fruiting body.

【００６１】実施例２１ 針葉樹オガクズ１００ｇ、マメカワ７０ｇをよく混合し
て水道水により水分含有率を６３％に調製したものを、
ポリプロピレン製８５０ml容広口ビンに圧詰めして、ビ
ン口部中央より下方に向かい直径１cmの穴を開けた後、
該培養基を１２０℃で６０分間高圧蒸気滅菌して、常温
まで冷却後にリオフィラム ウルマリウム Ｋ−１２５
７の固体種菌を接種した。暗所、２５℃、湿度５０〜６
０％の条件下で該培養基を２８日間培養すると、ビン全
体に菌糸がまん延した。更に、同条件下で５２日間培養
を続けて子実体発生基を得た。該子実体発生基の上部菌
糸層１cmを除去し、水道水２０mlを加え充分に給水させ
た後に余剰の水道水を捨て、１５℃、湿度９０〜９５
％、照度２０ルックスの条件下で１０日間培養を続けて
子実体原基を得て、更に照度を２００ルックスに上げて
１４日間培養を続けて子実体を得た。得られた子実体の
収量は１５７ｇで、子実体の官能検査において苦味は感
じられなかった。Example 21 100 g of coniferous sawdust and 70 g of Mamekawa were mixed well and the water content was adjusted to 63% with tap water.
After compressing it into a polypropylene 850 ml wide-mouthed bottle, punch a hole with a diameter of 1 cm downward from the center of the bottle mouth,
The culture medium was sterilized by high-pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and then Lyophilum ulmarium K-125.
7 solid inoculums were inoculated. Dark place, 25 ° C, humidity 50-6
When the culture medium was cultivated for 28 days under the condition of 0%, the mycelium spread over the entire bottle. Further, the culture was continued for 52 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water was discarded, and the temperature was 15 ° C. and the humidity was 90 to 95.
%, And the illuminance was 20 lux, the culture was continued for 10 days to obtain a fruit body primordium, and the illuminance was further increased to 200 lux to continue the culture for 14 days to obtain a fruit body. The yield of the fruiting body obtained was 157 g, and no bitterness was felt in the sensory test of the fruiting body.

【００６２】実施例２２ 液体ＰＧＹ培地１００mlに、リオフィラム ウルマリウ
ム Ｋ−６８０４（ＦＥＲＭ Ｐ−１２９８４）を接種
し、２５℃で１０日間培養して液体種菌を得た。一方、
コーンコブミール１３０ｇ、マメカワ６０ｇ、フスマ３
０ｇをよく混合して水道水により水分含有率を６２％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後に前記液体種菌２０mlを接
種した。暗所、２５℃、湿度５０〜６０％の条件下で該
培養基を３０日間培養すると、ビン全体に菌糸がまん延
した。更に、同条件下で３０日間培養を続けて子実体発
生基を得た。該子実体発生基の上部菌糸層１cmを除去
し、水道水２０mlを加え充分に給水させた後に余剰の水
道水を捨て、１５℃、湿度９０〜９５％、照度２０ルッ
クスの条件下で１０日間培養を続けて子実体原基を得
て、更に照度を２００ルックスに上げて１６日間培養を
続けて子実体を得た。得られた子実体の収量は１９５ｇ
で、子実体の官能検査において苦味は感じられなかっ
た。Example 22 100 ml of liquid PGY medium was inoculated with riophyllum ulmarium K-6804 (FERM P-12984) and cultured at 25 ° C. for 10 days to obtain a liquid inoculum. on the other hand,
Corncob meal 130g, Mamekawa 60g, Bran 3
0 g was mixed well and the water content was adjusted to 62% with tap water. It was pressed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the mouth of the bottle.
After puncturing, the culture medium was sterilized by high pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with 20 ml of the liquid inoculum. When the culture medium was cultured for 30 days in the dark at 25 ° C. and a humidity of 50 to 60%, the mycelium spread to the entire bottle. Furthermore, the culture was continued for 30 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water is discarded, and the conditions are 15 ° C, humidity 90 to 95%, and illuminance 20 lux for 10 days. The culturing was continued to obtain a fruiting body primordium, the illuminance was further raised to 200 lux, and the cultivation was continued for 16 days to obtain a fruiting body. The yield of the fruiting body obtained is 195 g.
No bitterness was felt in the sensory test of fruiting bodies.

【００６３】実施例２３ 針葉樹オガクズ５０ｇ、綿実殻粉砕物５０ｇ、米糠１０
０ｇをよく混合して水道水により水分含有率を６３％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後にリオフィラム ウルマリ
ウム Ｋ−６８０４の固体種菌を接種した。暗所、２５
℃、湿度５０〜６０％の条件下で該培養基を２８日間培
養すると、ビン全体に菌糸がまん延した。更に、同条件
下で３２日間培養を続けて子実体発生基を得た。該子実
体発生基の上部菌糸層１cmを除去し、水道水２０mlを加
え充分に給水させた後に余剰の水道水を捨て、１５℃、
湿度９０〜９５％、照度２０ルックスの条件下で１０日
間培養を続けて子実体原基を得て、更に照度を２００ル
ックスに上げて１５日間培養を続けて子実体を得た。得
られた子実体の収量は１６３ｇで、子実体の官能検査に
おいて苦味は感じられなかった。Example 23 50 g of coniferous sawdust, 50 g of crushed cottonseed shell, 10 rice bran
0 g was mixed well and the water content was adjusted to 63% with tap water. It was packed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the bottle mouth.
After puncturing, the culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with a solid inoculum of Riofilum ulmarium K-6804. Dark place, 25
When the culture medium was cultured for 28 days under the condition of the temperature of 50 ° C. and the humidity of 50 to 60%, the mycelia were spread over the entire bottle. Further, the culture was continued for 32 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating base and adding 20 ml of tap water to supply water sufficiently, the surplus tap water was discarded and the temperature was raised to 15 ° C.
Cultivation was continued for 10 days under conditions of humidity of 90 to 95% and illuminance of 20 lux to obtain fruit body primordium. Further, the illuminance was increased to 200 lux and culturing was continued for 15 days to obtain fruit body. The yield of the fruiting body thus obtained was 163 g, and no bitterness was felt in the sensory test of the fruiting body.

【００６４】実施例２４ 針葉樹オガクズ１００ｇ、マメカワ７０ｇをよく混合し
て水道水により水分含有率を６３％に調製したものを、
ポリプロピレン製８５０ml容広口ビンに圧詰めして、ビ
ン口部中央より下方に向かい直径１cmの穴を開けた後、
該培養基を１２０℃で６０分間高圧蒸気滅菌して、常温
まで冷却後にリオフィラム ウルマリウム Ｋ−６８０
４の固体種菌を接種した。暗所、２５℃、湿度５０〜６
０％の条件下で該培養基を２８日間培養すると、ビン全
体に菌糸がまん延した。更に、同条件下で３２日間培養
を続けて子実体発生基を得た。該子実体発生基の上部菌
糸層１cmを除去し、水道水２０mlを加え充分に給水させ
た後に余剰の水道水を捨て、１５℃、湿度９０〜９５
％、照度２０ルックスの条件下で９日間培養を続けて子
実体原基を得て、更に照度を２００ルックスに上げて１
６日間培養を続けて子実体を得た。得られた子実体の収
量は１７１ｇで、子実体の官能検査において苦味は感じ
られなかった。Example 24 100 g of coniferous sawdust and 70 g of Mamekawa were mixed well and the water content was adjusted to 63% with tap water.
After compressing it into a polypropylene 850 ml wide-mouthed bottle, punch a hole with a diameter of 1 cm downward from the center of the bottle mouth,
The culture medium was sterilized by high-pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and then Lyophilum ulmarium K-680.
4 solid inoculums were inoculated. Dark place, 25 ° C, humidity 50-6
When the culture medium was cultivated for 28 days under the condition of 0%, the mycelium spread over the entire bottle. Further, the culture was continued for 32 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water was discarded, and the temperature was 15 ° C. and the humidity was 90 to 95.
%, Culturing was continued for 9 days under the condition of 20 lux to obtain fruit body primordia.
Cultivation was continued for 6 days to obtain fruiting bodies. The yield of the fruiting body thus obtained was 171 g, and no bitterness was felt in the sensory test of the fruiting body.

【００６５】実施例２５ 液体ＰＧＹ培地１００mlに、リオフィラム ウルマリウ
ム Ｋ−６８０６（ＦＥＲＭ Ｐ−１２９８５）を接種
し、２５℃で１０日間培養して液体種菌を得た。一方、
コーンコブミール１３０ｇ、マメカワ６０ｇ、フスマ３
０ｇをよく混合して水道水により水分含有率を６２％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後に前記液体種菌２０mlを接
種した。暗所、２５℃、湿度５０〜６０％の条件下で該
培養基を３２日間培養すると、ビン全体に菌糸がまん延
した。更に、同条件下で３８日間培養を続けて子実体発
生基を得た。該子実体発生基の上部菌糸層１cmを除去
し、水道水２０mlを加え充分に給水させた後に余剰の水
道水を捨て、１５℃、湿度９０〜９５％、照度２０ルッ
クスの条件下で９日間培養を続けて子実体原基を得て、
更に照度を２００ルックスに上げて１５日間培養を続け
て子実体を得た。得られた子実体の収量は１９１ｇで、
子実体の官能検査において苦味は感じられなかった。Example 25 100 ml of a liquid PGY medium was inoculated with lyophilum ulmarium K-6806 (FERM P-12985) and cultured at 25 ° C. for 10 days to obtain a liquid inoculum. on the other hand,
Corncob meal 130g, Mamekawa 60g, Bran 3
0 g was mixed well and the water content was adjusted to 62% with tap water. It was pressed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the mouth of the bottle.
After puncturing, the culture medium was sterilized by high pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with 20 ml of the liquid inoculum. When the culture medium was cultured for 32 days in a dark place at 25 ° C. and a humidity of 50 to 60%, hyphae spread to the entire bottle. Further, the culture was continued for 38 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water is discarded, and the conditions are 15 ° C., humidity 90 to 95%, and illuminance 20 Lux for 9 days. Continue culturing to obtain fruit body primordia,
The illuminance was further raised to 200 lux and the culture was continued for 15 days to obtain fruiting bodies. The yield of the fruiting body obtained was 191 g,
No bitterness was felt in the sensory test of fruiting bodies.

【００６６】実施例２６ 針葉樹オガクズ５０ｇ、綿実殻粉砕物５０ｇ、米糠１０
０ｇをよく混合して水道水により水分含有率を６３％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後にリオフィラム ウルマリ
ウム Ｋ−６８０６の固体種菌を接種した。暗所、２５
℃、湿度５０〜６０％の条件下で該培養基を３０日間培
養すると、ビン全体に菌糸がまん延した。更に、同条件
下で４０日間培養を続けて子実体発生基を得た。該子実
体発生基の上部菌糸層１cmを除去し、水道水２０mlを加
え充分に給水させた後に余剰の水道水を捨て、１５℃、
湿度９０〜９５％、照度２０ルックスの条件下で９日間
培養を続けて子実体原基を得て、更に照度を２００ルッ
クスに上げて１５日間培養を続けて子実体を得た。得ら
れた子実体の収量は１６２ｇで、子実体の官能検査にお
いて苦味は感じられなかった。Example 26 50 g of softwood sawdust, 50 g of crushed cottonseed shell, 10 rice bran
0 g was mixed well and the water content was adjusted to 63% with tap water. It was packed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the bottle mouth.
After puncturing, the culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with a solid inoculum of Riofilum ulmarium K-6806. Dark place, 25
When the culture medium was cultured for 30 days at a temperature of 50 ° C. and a humidity of 50% to 60%, the mycelium spread to the entire bottle. Further, the culture was continued for 40 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating base and adding 20 ml of tap water to supply water sufficiently, the surplus tap water was discarded and the temperature was raised to 15 ° C.
Cultivation was continued for 9 days under the conditions of humidity of 90 to 95% and illumination of 20 lux to obtain fruit body primordium, and the illuminance was further raised to 200 lux to continue culturing for 15 days to obtain fruiting bodies. The yield of the fruiting body obtained was 162 g, and no bitterness was felt in the sensory test of the fruiting body.

【００６７】実施例２７ 針葉樹オガクズ１００ｇ、マメカワ７０ｇをよく混合し
て水道水により水分含有率を６３％に調製したものを、
ポリプロピレン製８５０ml容広口ビンに圧詰めして、ビ
ン口部中央より下方に向かい直径１cmの穴を開けた後、
該培養基を１２０℃で６０分間高圧蒸気滅菌して、常温
まで冷却後にリオフィラム ウルマリウム Ｋ−６８０
６の固体種菌を接種した。暗所、２５℃、湿度５０〜６
０％の条件下で該培養基を３１日間培養すると、ビン全
体に菌糸がまん延した。更に、同条件下で３９日間培養
を続けて子実体発生基を得た。該子実体発生基の上部菌
糸層１cmを除去し、水道水２０mlを加え充分に給水させ
た後に余剰の水道水を捨て、１５℃、湿度９０〜９５
％、照度２０ルックスの条件下で９日間培養を続けて子
実体原基を得て、更に照度を２００ルックスに上げて１
４日間培養を続けて子実体を得た。得られた子実体の収
量は１６５ｇで、子実体の官能検査において苦味は感じ
られなかった。Example 27 100 g of coniferous sawdust and 70 g of Mamekawa were mixed well and the water content was adjusted to 63% with tap water.
After compressing it into a polypropylene 850 ml wide-mouthed bottle, punch a hole with a diameter of 1 cm downward from the center of the bottle mouth,
The culture medium was sterilized by high-pressure steam at 120 ° C. for 60 minutes, cooled to room temperature, and then Lyophilum ulmarium K-680.
6 solid inoculums were inoculated. Dark place, 25 ° C, humidity 50-6
When the culture medium was cultured for 31 days under the condition of 0%, the mycelium spread to the entire bottle. Further, the culture was continued for 39 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water was discarded, and the temperature was 15 ° C. and the humidity was 90 to 95.
%, Culturing was continued for 9 days under the condition of 20 lux to obtain fruit body primordia.
Cultivation was continued for 4 days to obtain fruiting bodies. The yield of the fruiting body thus obtained was 165 g, and no bitterness was felt in the sensory test of the fruiting body.

【００６８】対照例１ コーンコブミール１３０ｇ、マメカワ６０ｇ、フスマ３
０ｇをよく混合して水道水により水分含有率を６２％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後にリオフィラム ウルマリ
ウム Ｌｕ １−２の固体種菌を接種した。暗所、２５
℃、湿度５０〜６０％の条件下で該培養基を３５日間培
養すると、ビン全体に菌糸がまん延した。更に、同条件
下で５０日間培養を続けて子実体発生基を得た。該子実
体発生基の上部菌糸層１cmを除去し、水道水２０mlを加
え充分に給水させた後に余剰の水道水を捨て、１５℃、
湿度９０〜９５％、照度２０ルックスの条件下で９日間
培養を続けて子実体原基を得て、更に照度を２００ルッ
クスに上げて１３日間培養を続けて子実体を得た。得ら
れた子実体の収量は１７８ｇで、子実体の官能検査にお
いて苦味が感じられた。Control Example 1 130 g of corn cob meal, 60 g of mamekawa, 3 of fusuma
0 g was mixed well and the water content was adjusted to 62% with tap water. It was pressed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the mouth of the bottle.
After puncturing, the culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with a solid inoculum of Riofilum ulmarium Lu 1-2. Dark place, 25
When the culture medium was cultivated for 35 days at 50 ° C. and a humidity of 50 to 60%, mycelia spread to the entire bottle. Furthermore, the culture was continued for 50 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating base and adding 20 ml of tap water to supply water sufficiently, the surplus tap water was discarded and the temperature was raised to 15 ° C.
Cultivation was continued for 9 days under conditions of humidity of 90 to 95% and illuminance of 20 lux to obtain fruit body primordia, and the illuminance was further raised to 200 lux for 13 days of culturing to obtain fruit bodies. The yield of the fruiting body thus obtained was 178 g, and bitterness was felt in the sensory test of the fruiting body.

【００６９】対照例２ 針葉樹オガクズ５０ｇ、綿実殻粉砕物５０ｇ、米糠１０
０ｇをよく混合して水道水により水分含有率を６３％に
調製したものを、ポリプロピレン製８５０ml容広口ビン
に圧詰めして、ビン口部中央より下方に向かい直径１cm
の穴を開けた後、該培養基を１２０℃で６０分間高圧蒸
気滅菌して、常温まで冷却後にリオフィラム ウルマリ
ウム Ｌｕ １−２の固体種菌を接種した。暗所、２５
℃、湿度５０〜６０％の条件下で該培養基を３０日間培
養すると、ビン全体に菌糸がまん延した。更に、同条件
下で５５日間培養を続けて子実体発生基を得た。該子実
体発生基の上部菌糸層１cmを除去し、水道水２０mlを加
え充分に給水させた後に余剰の水道水を捨て、１５℃、
湿度９０〜９５％、照度２０ルックスの条件下で９日間
培養を続けて子実体原基を得て、更に照度を２００ルッ
クスに上げて１４日間培養を続けて子実体を得た。得ら
れた子実体の収量は１４０ｇで、子実体の官能検査にお
いて苦味が感じられた。Control Example 2 50 g of coniferous sawdust, 50 g of crushed cottonseed shell, 10 rice bran
0 g was mixed well and the water content was adjusted to 63% with tap water. It was packed into a polypropylene 850 ml wide mouth bottle, and the diameter was 1 cm downward from the center of the bottle mouth.
After puncturing, the culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with a solid inoculum of Riofilum ulmarium Lu 1-2. Dark place, 25
When the culture medium was cultured for 30 days at a temperature of 50 ° C. and a humidity of 50% to 60%, the mycelium spread to the entire bottle. Further, cultivation was continued for 55 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating base and adding 20 ml of tap water to supply water sufficiently, the surplus tap water was discarded and the temperature was raised to 15 ° C.
Cultivation was continued for 9 days under conditions of humidity of 90 to 95% and illuminance of 20 lux to obtain a fruiting body primordium. Further, the illuminance was increased to 200 lux and culturing was continued for 14 days to obtain fruiting bodies. The yield of the fruiting body obtained was 140 g, and bitterness was felt in the sensory test of the fruiting body.

【００７０】対照例３ 針葉樹オガクズ１００ｇ、マメカワ７０ｇをよく混合し
て水道水により水分含有率を６３％に調製したものを、
ポリプロピレン製８５０ml容広口ビンに圧詰めして、ビ
ン口部中央より下方に向かい直径１cmの穴を開けた後、
該培養基を１２０℃で６０分間高圧蒸気滅菌して、常温
まで冷却後にリオフィラム ウルマリウム Ｌｕ １−
２の固体種菌を接種した。暗所、２５℃、湿度５０〜６
０％の条件下で該培養基を３０日間培養すると、ビン全
体に菌糸がまん延した。更に、同条件下で５５日間培養
を続けて子実体発生基を得た。該子実体発生基の上部菌
糸層１cmを除去し、水道水２０mlを加え充分に給水させ
た後に余剰の水道水を捨て、１５℃、湿度９０〜９５
％、照度２０ルックスの条件下で１０日間培養を続けて
子実体原基を得て、更に照度を２００ルックスに上げて
１３日間培養を続けて子実体を得た。得られた子実体の
収量は１５３ｇで、子実体の官能検査において苦味が感
じられた。Comparative Example 3 100 g of coniferous sawdust and 70 g of Mamekawa were mixed well and the water content was adjusted to 63% with tap water.
After compressing it into a polypropylene 850 ml wide-mouthed bottle, punch a hole with a diameter of 1 cm downward from the center of the bottle mouth,
The culture medium was sterilized by high-pressure steam at 120 ° C for 60 minutes, cooled to room temperature, and then lyophilum ulmarium Lu 1-
Two solid inoculums were inoculated. Dark place, 25 ° C, humidity 50-6
When the culture medium was cultivated for 30 days under the condition of 0%, the mycelium spread over the entire bottle. Further, cultivation was continued for 55 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water was discarded, and the temperature was 15 ° C. and the humidity was 90 to 95.
%, And the illuminance was 20 lux, the culture was continued for 10 days to obtain fruit body primordia, and the illuminance was further increased to 200 lux to continue culture for 13 days to obtain fruit bodies. The yield of the fruiting body thus obtained was 153 g, and bitterness was felt in the sensory test of the fruiting body.

【００７１】[0071]

【発明の効果】以上説明した様に、本発明によれば、従
来使用することができなかった高苦味化培地を用い栽培
しても、子実体の苦味が低減したリオフィラム ウルマ
リウム子実体を得ることが可能となり、工業的に高収量
で良質なリオフィラム ウルマリウム子実体及び菌糸体
を提供することができる。As described above, according to the present invention, it is possible to obtain Riofilum ulmarium fruiting bodies with reduced bitterness of fruiting bodies even when cultivated using a high bittering medium which could not be used conventionally. It is possible to provide lyophilum ulmarium fruiting bodies and mycelium of high quality with high yield industrially.

───────────────────────────────────────────────────── フロントページの続き 微生物の受託番号 ＦＥＲＭ Ｐ−１２９８５ 前置審査 (72)発明者 木野 真奈美 滋賀県大津市瀬田３丁目４番１号 寳酒 造株式会社中央研究所内 (72)発明者 松井 侑 滋賀県大津市瀬田３丁目４番１号 寳酒 造株式会社中央研究所内 (72)発明者 森田 日出男 滋賀県大津市瀬田３丁目４番１号 寳酒 造株式会社中央研究所内 (58)調査した分野(Int.Cl.⁷，ＤＢ名) C12N 1/14 A01G 1/04 A01H 15/00 ─────────────────────────────────────────────────── ─── Continuation of front page microbial consignment number FERM P-12985 Preliminary examination (72) Inventor Manami Kino 3-4-1 Seta 3-chome Seta, Otsu City, Shiga Prefecture In-house Research Laboratories (72) Inventor Matsui YU, Central 3-chome, Seta 3-chome, Otsu City, Shiga Prefecture Inventor, Hideo Morita (72) Inventor Hideo Morita 3-chome 4-chome Seta, Otsu City, Shiga Prefecture (58) Survey Areas (Int.Cl. ⁷ , DB name) C12N 1/14 A01G 1/04 A01H 15/00

Claims (2)

(57)【特許請求の範囲】(57) [Claims] 【請求項１】 高苦味化培地で栽培して得られる子実体
の苦味が、少なくとも一種の高苦味化培地について、
０．０００００８Ｍ 硫酸キニーネよりも苦くなく、か
つ下記の菌株、又はこれらの変異株から選択されるリオ
フィラム ウルマリウム新菌株を培地に接種し、菌糸体
を生成させることを特徴とするリオフィラム ウルマリ
ウム新菌株の培養方法。 リオフィラム ウルマリウム Ｋ−０４２９（ＦＥＲＭ
Ｐ−１２５７０）、 リオフィラム ウルマリウム Ｋ−０２０７（ＦＥＲＭ
Ｐ−１２５６９）、 リオフィラム ウルマリウム Ｋ−０２０２（ＦＥＲＭ
Ｐ−１２９８０）、 リオフィラム ウルマリウム Ｋ−０２５９（ＦＥＲＭ
Ｐ−１２９８１）、 リオフィラム ウルマリウム Ｋ−１００４（ＦＥＲＭ
Ｐ−１２９８２）、 リオフィラム ウルマリウム Ｋ−１２５７（ＦＥＲＭ
Ｐ−１２９８３）、 リオフィラム ウルマリウム Ｋ−６８０４（ＦＥＲＭ
Ｐ−１２９８４）、 リオフィラム ウルマリウム Ｋ−６８０６（ＦＥＲＭ
Ｐ−１２９８５）1. The bitterness of fruiting bodies obtained by culturing in a high bittering medium is at least one high bittering medium,
Culture of a new strain of Riofilum ulmarium, which is less bitter than 0.000008M quinine sulfate and inoculates a medium with a new strain of Riofilum ulmarium selected from the following strains or mutants thereof to produce mycelia: Method. Riofilum Ulmarium K-0429 (FERM
P-12570), Riofilum ulmarium K-0207 (FERM
P-12569), Riofilum ulmarium K-0202 (FERM
P-12980), Riofilum ulmarium K-0259 (FERM
P-12981), Riofilum ulmarium K-1004 (FERM
P-12982), Riofilum ulmarium K-1257 (FERM
P-12983), Riofilum ulmarium K-6804 (FERM
P-12984), Riofilum ulmarium K-6806 (FERM
P-12985) 【請求項２】 高苦味化培地で栽培して得られる子実体
の苦味が、少なくとも一種の高苦味化培地について、
０．０００００８Ｍ 硫酸キニーネよりも苦くなく、か
つ下記の菌株、又はこれらの変異株から選択されるリオ
フィラム ウルマリウム新菌株を培地に接種し、子実体
を生成させることを特徴とするリオフィラム ウルマリ
ウム新菌株の子実体の栽培方法。 リオフィラム ウルマリウム Ｋ−０４２９（ＦＥＲＭ
Ｐ−１２５７０）、 リオフィラム ウルマリウム Ｋ−０２０７（ＦＥＲＭ
Ｐ−１２５６９）、 リオフィラム ウルマリウム Ｋ−０２０２（ＦＥＲＭ
Ｐ−１２９８０）、 リオフィラム ウルマリウム Ｋ−０２５９（ＦＥＲＭ
Ｐ−１２９８１）、 リオフィラム ウルマリウム Ｋ−１００４（ＦＥＲＭ
Ｐ−１２９８２）、 リオフィラム ウルマリウム Ｋ−１２５７（ＦＥＲＭ
Ｐ−１２９８３）、 リオフィラム ウルマリウム Ｋ−６８０４（ＦＥＲＭ
Ｐ−１２９８４）、 リオフィラム ウルマリウム Ｋ−６８０６（ＦＥＲＭ
Ｐ−１２９８５）2. The bitterness of fruiting bodies obtained by culturing in a high bittering medium is at least one high bittering medium,
A child of a new strain of Riofilum ulmarium, which is less bitter than 0.000008M quinine sulfate and inoculates a medium with a new strain of Riofilum ulmarium selected from the following strains or mutants thereof to produce fruiting bodies. Cultivation method of substance. Riofilum Ulmarium K-0429 (FERM
P-12570), Riofilum ulmarium K-0207 (FERM
P-12569), Riofilum ulmarium K-0202 (FERM
P-12980), Riofilum ulmarium K-0259 (FERM
P-12981), Riofilum ulmarium K-1004 (FERM
P-12982), Riofilum ulmarium K-1257 (FERM
P-12983), Riofilum ulmarium K-6804 (FERM
P-12984), Riofilum ulmarium K-6806 (FERM
P-12985)